##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765273_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7258503 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4529440850269 33.0 33.0 33.0 27.0 33.0 2 31.293165408900432 33.0 33.0 33.0 27.0 33.0 3 31.396945210327804 33.0 33.0 33.0 27.0 33.0 4 35.02517337252599 37.0 37.0 37.0 33.0 37.0 5 35.12421390471286 37.0 37.0 37.0 33.0 37.0 6 35.05831794792948 37.0 37.0 37.0 33.0 37.0 7 34.98039017136178 37.0 37.0 37.0 33.0 37.0 8 34.9033724998116 37.0 37.0 37.0 33.0 37.0 9 34.8603035639718 37.0 37.0 37.0 33.0 37.0 10-11 34.84392008930767 37.0 37.0 37.0 33.0 37.0 12-13 34.784798532149125 37.0 37.0 37.0 33.0 37.0 14-15 36.25761902970902 37.0 37.0 40.0 33.0 40.0 16-17 36.30746243405837 37.0 37.0 40.0 33.0 40.0 18-19 36.31235049430991 37.0 37.0 40.0 33.0 40.0 20-21 36.31371654733765 37.0 37.0 40.0 33.0 40.0 22-23 36.35777866317614 37.0 37.0 40.0 33.0 40.0 24-25 36.317898263595126 37.0 37.0 40.0 33.0 40.0 26-27 36.32765151436874 37.0 37.0 40.0 33.0 40.0 28-29 36.2574833956809 37.0 37.0 40.0 33.0 40.0 30-31 36.222471010895774 37.0 37.0 40.0 30.0 40.0 32-33 36.098203513865045 37.0 37.0 40.0 27.0 40.0 34-35 36.047849260377795 37.0 37.0 40.0 27.0 40.0 36-37 35.93679419847315 37.0 37.0 40.0 27.0 40.0 38-39 35.73215186382096 37.0 37.0 40.0 27.0 40.0 40-41 35.64803190134384 37.0 37.0 40.0 27.0 40.0 42-43 35.51720892035176 37.0 35.0 40.0 27.0 40.0 44-45 35.37513974989058 37.0 33.0 40.0 27.0 40.0 46-47 35.24821846873935 37.0 33.0 40.0 27.0 40.0 48-49 35.087931147786264 37.0 33.0 40.0 27.0 40.0 50-51 34.925602772362296 37.0 33.0 40.0 27.0 40.0 52-53 34.74755614208605 37.0 33.0 40.0 27.0 40.0 54-55 34.61212739045503 37.0 33.0 40.0 27.0 40.0 56-57 34.4187326918512 37.0 33.0 40.0 27.0 40.0 58-59 34.19132939670894 37.0 33.0 37.0 27.0 40.0 60-61 33.96125633618943 37.0 33.0 37.0 27.0 40.0 62-63 33.78164802025982 37.0 33.0 37.0 27.0 40.0 64-65 33.478093072359414 37.0 33.0 37.0 24.5 40.0 66-67 33.24872070728634 37.0 33.0 37.0 22.0 40.0 68-69 32.95365366660315 37.0 33.0 37.0 22.0 40.0 70-71 32.79166792381294 37.0 33.0 37.0 22.0 37.0 72-73 32.516698553406954 35.0 33.0 37.0 22.0 37.0 74-75 32.246337640144255 33.0 33.0 37.0 22.0 37.0 76-77 32.05828047463781 33.0 33.0 37.0 22.0 37.0 78-79 31.827457534976563 33.0 33.0 37.0 22.0 37.0 80-81 31.57015509947437 33.0 33.0 37.0 22.0 37.0 82-83 31.40453651393407 33.0 30.0 37.0 22.0 37.0 84-85 31.229690336974443 33.0 27.0 37.0 22.0 37.0 86-87 31.03124666339602 33.0 27.0 37.0 22.0 37.0 88-89 30.862160007373422 33.0 27.0 37.0 18.5 37.0 90-91 30.677013428250977 33.0 27.0 37.0 15.0 37.0 92-93 30.546993229871227 33.0 27.0 37.0 15.0 37.0 94-95 30.32920080077118 33.0 27.0 37.0 15.0 37.0 96-97 30.251759901456264 33.0 27.0 37.0 15.0 37.0 98-99 29.105961174087824 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2368934507933389 1101 2 0.26163918336412806 1101 3 0.3073862366209603 1101 4 0.3802936774631789 1101 5 0.3101685986379934 1101 6 0.2299969108207307 1101 7 0.17735870100261764 1101 8 0.23996732809935395 1101 9 0.20689456536334205 1101 10-11 0.2638720130263792 1101 12-13 0.24275902129525662 1101 14-15 0.3703189531182929 1101 16-17 0.29661469652308625 1101 18-19 0.256240753707921 1101 20-21 0.2621908490716294 1101 22-23 0.2181282884737783 1101 24-25 0.26466622277541774 1101 26-27 0.31051151291031687 1101 28-29 0.26699679381304264 1101 30-31 0.29407914361484444 1101 32-33 0.3256909828109258 1101 34-35 0.2770596228627227 1101 36-37 0.30881755879801176 1101 38-39 0.23169114765570953 1101 40-41 0.27333647788698556 1101 42-43 0.261963552234171 1101 44-45 0.24941621978086914 1101 46-47 0.26282368526840827 1101 48-49 0.28146242317214387 1101 50-51 0.22480773095947626 1101 52-53 0.17819835169141385 1101 54-55 0.31804099476081404 1101 56-57 0.27993994398085675 1101 58-59 0.2076338500247772 1101 60-61 0.3102647569573449 1101 62-63 0.30161745658953976 1101 64-65 0.17783905077818218 1101 66-67 0.24660362597035856 1101 68-69 0.2166062194951408 1101 70-71 0.25634497474449347 1101 72-73 0.2876910962654122 1101 74-75 0.3527600787516718 1101 76-77 0.25372253802729716 1101 78-79 0.2872990288441777 1101 80-81 0.1983641051637015 1101 82-83 0.2055471992482012 1101 84-85 0.2067336579691137 1101 86-87 0.24182836809775665 1101 88-89 0.25490104619785825 1101 90-91 0.2975170710333188 1101 92-93 0.40035942941984715 1101 94-95 0.36525740914395755 1101 96-97 0.29667841347772494 1101 98-99 0.3209489486726973 1102 1 0.20661163493154788 1102 2 0.2095491128143152 1102 3 0.20163844760461203 1102 4 0.2529257508332279 1102 5 0.18576695297672075 1102 6 0.20806880707900888 1102 7 0.16278699162846522 1102 8 0.2538261416579033 1102 9 0.2638425592175295 1102 10-11 0.15415554994491032 1102 12-13 0.20935246563983867 1102 14-15 0.20355146078914998 1102 16-17 0.22762651742981888 1102 18-19 0.221394708904036 1102 20-21 0.22155554268215383 1102 22-23 0.16952026699823364 1102 24-25 0.27003928926782095 1102 26-27 0.30530330642539383 1102 28-29 0.1800342968046209 1102 30-31 0.19540054867947987 1102 32-33 0.31315981504775436 1102 34-35 0.1995828507949824 1102 36-37 0.3118670551397926 1102 38-39 0.2972922735882477 1102 40-41 0.2529442948287013 1102 42-43 0.2611866192586021 1102 44-45 0.20901918432220157 1102 46-47 0.27029377240724983 1102 48-49 0.22350687642924072 1102 50-51 0.2202175510490889 1102 52-53 0.22418398264111516 1102 54-55 0.31448686060182496 1102 56-57 0.28628325409484745 1102 58-59 0.19294360899068863 1102 60-61 0.3581536165520518 1102 62-63 0.3346900951485452 1102 64-65 0.20625737092885288 1102 66-67 0.20234968379617158 1102 68-69 0.21075889969053918 1102 70-71 0.24840809107998751 1102 72-73 0.22359030449486994 1102 74-75 0.16469567474821645 1102 76-77 0.2517957060772673 1102 78-79 0.28682203096163406 1102 80-81 0.2480897410215377 1102 82-83 0.23453202405425344 1102 84-85 0.15615177527492463 1102 86-87 0.2640345179283301 1102 88-89 0.3321519950169396 1102 90-91 0.23858879788826926 1102 92-93 0.331090907243464 1102 94-95 0.3046491802633433 1102 96-97 0.26409110785030876 1102 98-99 0.22531420048660422 1103 1 0.09074674586101494 1103 2 0.12270627185110072 1103 3 0.13367403436210168 1103 4 0.19434225860765508 1103 5 0.16747370020277685 1103 6 0.09673138986300245 1103 7 0.11720468389749783 1103 8 0.07331510495721005 1103 9 0.14871387730808294 1103 10-11 0.11329495420968527 1103 12-13 0.08074778630373203 1103 14-15 0.10003456185526005 1103 16-17 0.08011493790510116 1103 18-19 0.1317783566881232 1103 20-21 0.16428373927830364 1103 22-23 0.13670542324069856 1103 24-25 0.20970956610807434 1103 26-27 0.27910524122194147 1103 28-29 0.18297474890946575 1103 30-31 0.26027969295451925 1103 32-33 0.30006193066360964 1103 34-35 0.265838666350632 1103 36-37 0.2957556321054824 1103 38-39 0.24159559173737222 1103 40-41 0.21403970438792896 1103 42-43 0.17741132477407717 1103 44-45 0.1459833286488248 1103 46-47 0.07851801397625735 1103 48-49 0.12194838739038971 1103 50-51 0.17511291015330954 1103 52-53 0.09488009703952827 1103 54-55 0.15657682295026376 1103 56-57 0.11383109899028909 1103 58-59 0.18069088763181185 1103 60-61 0.19256844326937994 1103 62-63 0.21993187981813378 1103 64-65 0.1523015283817628 1103 66-67 0.209301533874239 1103 68-69 0.13129904786718782 1103 70-71 0.023363926305115967 1103 72-73 0.20546639717366588 1103 74-75 0.15855771956731246 1103 76-77 0.11563751064930017 1103 78-79 0.153575590519349 1103 80-81 0.15551063798249132 1103 82-83 0.15208293751958735 1103 84-85 0.1596272135695429 1103 86-87 0.2739125173139456 1103 88-89 0.223179440079722 1103 90-91 0.19891119908081834 1103 92-93 0.2646661456150987 1103 94-95 0.19039499085004508 1103 96-97 0.08648925512635586 1103 98-99 0.20120784658331559 1104 1 0.13887115686913987 1104 2 0.10089941777556533 1104 3 0.10416823800078134 1104 4 0.08314805569192174 1104 5 0.07728865227003467 1104 6 0.037269683833706324 1104 7 0.03197866143658956 1104 8 0.0803502336010169 1104 9 0.022839948878917937 1104 10-11 0.05599031977585156 1104 12-13 0.0678657155403215 1104 14-15 0.058769762536520886 1104 16-17 0.02193059485531279 1104 18-19 -0.038482242450392334 1104 20-21 0.051489003472653394 1104 22-23 0.07903322639558752 1104 24-25 0.0883435545525657 1104 26-27 0.08807605614266123 1104 28-29 0.009088648373065666 1104 30-31 0.03153616747167831 1104 32-33 0.09854956894292144 1104 34-35 0.15611811123015684 1104 36-37 0.17777603959632415 1104 38-39 0.1007990158667269 1104 40-41 0.13746966156690377 1104 42-43 0.055783089297861466 1104 44-45 0.008049044641893488 1104 46-47 0.02135104626791673 1104 48-49 0.06534582910303754 1104 50-51 0.08602441112922321 1104 52-53 -0.04994329509990081 1104 54-55 0.13592775528107381 1104 56-57 0.07595086556002428 1104 58-59 0.12983085330583322 1104 60-61 0.11995681228756183 1104 62-63 0.057228504279002834 1104 64-65 0.03299361729829542 1104 66-67 0.10317328572158857 1104 68-69 0.03669290663027169 1104 70-71 -0.02739424680405733 1104 72-73 -0.011629793729873938 1104 74-75 -0.03494878360924503 1104 76-77 -0.08831182029991425 1104 78-79 -0.04866843124333897 1104 80-81 -0.06823196400307907 1104 82-83 -0.032241334990601445 1104 84-85 -0.04448039371930079 1104 86-87 0.0794767366717366 1104 88-89 0.026412186946753735 1104 90-91 0.05561714108952032 1104 92-93 0.022521848399026823 1104 94-95 0.02237881159082633 1104 96-97 -0.2288812343581874 1104 98-99 -0.12035125319473394 1105 1 0.13730832912584745 1105 2 0.15995894408218092 1105 3 0.15625412557762886 1105 4 0.14940870227127334 1105 5 0.14054772420442418 1105 6 0.1443189527907478 1105 7 0.08781651731964502 1105 8 0.09895645123290109 1105 9 0.08869437841725158 1105 10-11 0.0820214463412654 1105 12-13 0.04318544528042878 1105 14-15 0.12314703919839332 1105 16-17 0.0951493700592323 1105 18-19 0.11816317291022216 1105 20-21 0.12173574708913293 1105 22-23 0.08322464768254179 1105 24-25 0.18193743795793438 1105 26-27 0.20097189618473266 1105 28-29 0.10164040042409539 1105 30-31 0.12175918801296604 1105 32-33 0.15625454606182387 1105 34-35 0.10439264895516942 1105 36-37 0.10433569146093902 1105 38-39 0.05454714357545498 1105 40-41 0.011391709255306637 1105 42-43 0.047332297691632164 1105 44-45 0.08017546529367081 1105 46-47 0.0033091010348371697 1105 48-49 -0.006900240931734913 1105 50-51 0.11707711889815897 1105 52-53 0.022389926954581085 1105 54-55 0.13823802237985916 1105 56-57 0.1578493910660299 1105 58-59 0.08114241734484295 1105 60-61 0.14915864750229701 1105 62-63 0.1001132065883894 1105 64-65 0.05032135911560687 1105 66-67 0.1820052418560607 1105 68-69 0.06527212190757581 1105 70-71 0.044555654078983764 1105 72-73 0.09314392122097814 1105 74-75 0.1532988403903488 1105 76-77 0.005948997629182884 1105 78-79 0.0891248205314632 1105 80-81 0.08795036393076927 1105 82-83 0.09586179391462224 1105 84-85 0.028504252753702275 1105 86-87 0.06470963936576624 1105 88-89 0.03561337221421823 1105 90-91 0.0802043507181942 1105 92-93 0.12144308365335021 1105 94-95 0.04214902568936196 1105 96-97 -0.12089618905390509 1105 98-99 0.12401784004019945 1106 1 0.07390228364444695 1106 2 0.13343027145161912 1106 3 0.11038011384392732 1106 4 0.01625643622104178 1106 5 0.028071900268770378 1106 6 0.016455760764678473 1106 7 0.006196383712655518 1106 8 0.02933052791650681 1106 9 0.05532981723183639 1106 10-11 -0.030852851184867802 1106 12-13 0.08228670883426048 1106 14-15 0.043541671447805186 1106 16-17 0.06138146967044378 1106 18-19 0.08871419158780469 1106 20-21 0.07120414550436749 1106 22-23 0.08858870844167654 1106 24-25 0.11545074374444653 1106 26-27 0.11378721444020812 1106 28-29 0.02212978746882044 1106 30-31 0.03619960489159979 1106 32-33 0.0840145581420515 1106 34-35 -0.06371135729237665 1106 36-37 -4.98619059037253E-4 1106 38-39 0.02304125455929551 1106 40-41 0.09559952056333998 1106 42-43 0.0860098427642555 1106 44-45 0.03983179764517786 1106 46-47 -0.015482072907438749 1106 48-49 0.053508096422227425 1106 50-51 0.062107145439803446 1106 52-53 0.014828024616711843 1106 54-55 1.4460177582265032E-4 1106 56-57 0.08365028220826076 1106 58-59 0.15439504788014347 1106 60-61 0.19045480914011392 1106 62-63 0.11059198354089972 1106 64-65 0.02207021126525177 1106 66-67 0.055004115800429076 1106 68-69 0.023891242308998528 1106 70-71 -0.0032707793273587527 1106 72-73 0.14611311444412678 1106 74-75 0.03530997143287351 1106 76-77 0.029166109994569922 1106 78-79 0.07824699094031118 1106 80-81 0.03296506982352909 1106 82-83 0.0692431355936236 1106 84-85 -0.015174804599670466 1106 86-87 -0.0672345311924758 1106 88-89 0.09710357256423308 1106 90-91 0.053575333869243735 1106 92-93 0.10925261625276406 1106 94-95 0.008360376075323472 1106 96-97 -0.11312716441096882 1106 98-99 0.242260082773452 1107 1 0.0063533592008369055 1107 2 0.05996375402838083 1107 3 -0.02197900802536168 1107 4 -0.020571317212215945 1107 5 0.13039745507190048 1107 6 -0.009884571424763067 1107 7 -0.07315761543102184 1107 8 -0.06371780510131941 1107 9 -0.1012874496060121 1107 10-11 -0.010281477336434364 1107 12-13 -0.030059653865386338 1107 14-15 -0.06864910053118933 1107 16-17 -0.09361603058683698 1107 18-19 -0.1198751562683853 1107 20-21 -0.0664618634578602 1107 22-23 -0.11698346265634285 1107 24-25 -0.0014903493577875793 1107 26-27 0.05196981572266424 1107 28-29 0.06536845985450412 1107 30-31 -0.04937537087328536 1107 32-33 -0.10611326860004766 1107 34-35 -0.07270069574674665 1107 36-37 -0.10718149223218632 1107 38-39 -0.152468619900894 1107 40-41 -0.209604788141867 1107 42-43 -0.1842448387742408 1107 44-45 -0.08232410181116734 1107 46-47 -0.25869163456627575 1107 48-49 -0.19395310833306212 1107 50-51 -0.14852624235637535 1107 52-53 -0.19343021055857434 1107 54-55 -0.10069487662602938 1107 56-57 -0.09775509411260686 1107 58-59 -0.07099819309505051 1107 60-61 -0.03693308041636101 1107 62-63 -0.2164991290637701 1107 64-65 -0.2230677097365259 1107 66-67 0.0012351422132113044 1107 68-69 -0.09989914584673443 1107 70-71 -0.0973653197876132 1107 72-73 -0.03545484044234115 1107 74-75 -0.13558311326870154 1107 76-77 -0.15268451910205982 1107 78-79 -0.06349493269906148 1107 80-81 -0.2825583007063166 1107 82-83 -0.14510664225642955 1107 84-85 -0.26521006145378934 1107 86-87 -0.14449598839954803 1107 88-89 -0.07318107730026568 1107 90-91 -0.06524142387233667 1107 92-93 -0.06667016043731167 1107 94-95 -0.10964515639211214 1107 96-97 -0.20473652545513232 1107 98-99 0.19451540973471637 1108 1 -0.023411328020817024 1108 2 -0.0455039208633643 1108 3 0.01480727925842018 1108 4 0.010141952982863245 1108 5 0.019173421159216275 1108 6 -0.177447590994106 1108 7 -0.023294293941219735 1108 8 -0.10444227115986848 1108 9 -0.046147735542263035 1108 10-11 -0.11342404947367157 1108 12-13 -0.11176731150902697 1108 14-15 -0.0762318456965474 1108 16-17 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0.014070580440044722 2215 84-85 -0.02295694923229874 2215 86-87 0.035085549048325504 2215 88-89 -0.13143927860464544 2215 90-91 0.11115307191492718 2215 92-93 0.09137597119079288 2215 94-95 -0.017609896728217223 2215 96-97 -0.0039393313238811345 2215 98-99 -0.01676662337607482 2216 1 -0.09231277155620887 2216 2 -0.09111678340433116 2216 3 -0.08114275150580141 2216 4 -0.0882744732354297 2216 5 -0.11470485314719525 2216 6 -0.046207099478024816 2216 7 -0.01991646967397287 2216 8 -0.08508017010836255 2216 9 -0.09049470016345396 2216 10-11 -0.11123065517793407 2216 12-13 -0.08490180605052444 2216 14-15 -0.19046514140132587 2216 16-17 -0.15348816015745825 2216 18-19 -0.24891002272170937 2216 20-21 -0.19412975745746053 2216 22-23 -0.29461114929681287 2216 24-25 -0.2465840548419891 2216 26-27 -0.29919522219722694 2216 28-29 -0.1699074398204985 2216 30-31 -0.19219854812553194 2216 32-33 -0.1338174679787798 2216 34-35 -0.21837065016143242 2216 36-37 -0.22786680008992732 2216 38-39 -0.1599844766428049 2216 40-41 -0.17498345974953367 2216 42-43 -0.2021912040798668 2216 44-45 -0.14031027438160493 2216 46-47 -0.10693621010720022 2216 48-49 -0.01476257267037795 2216 50-51 -0.04318763390423186 2216 52-53 0.0978109187988494 2216 54-55 -0.006432787698201992 2216 56-57 0.09099515824370741 2216 58-59 -0.1397798719417409 2216 60-61 -0.045615940205784966 2216 62-63 -0.014808977944042567 2216 64-65 0.04861200217293771 2216 66-67 -0.01707780421712357 2216 68-69 -0.09505987773376745 2216 70-71 -0.08378016642566166 2216 72-73 -0.09237168670376406 2216 74-75 -0.008578061052858743 2216 76-77 -0.05050239333728257 2216 78-79 0.04920527704650368 2216 80-81 0.1544579315304908 2216 82-83 0.07442336849380382 2216 84-85 0.14689974761497737 2216 86-87 0.05921603869037284 2216 88-89 -0.022465481029652068 2216 90-91 0.1797219045844649 2216 92-93 0.26133271006276004 2216 94-95 0.2092864942076318 2216 96-97 0.26342548786475817 2216 98-99 0.34736700782172747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 175.0 8 1275.0 9 2546.0 10 3989.0 11 7464.0 12 13265.0 13 22337.0 14 31240.0 15 35415.0 16 37134.0 17 39567.0 18 41541.0 19 44137.0 20 47327.0 21 51317.0 22 56634.0 23 63360.0 24 72589.0 25 81405.0 26 91052.0 27 102523.0 28 123072.0 29 152174.0 30 191802.0 31 245924.0 32 325516.0 33 438330.0 34 599509.0 35 865050.0 36 1329060.0 37 1640120.0 38 501257.0 39 393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25234812707813 18.205751785050516 12.421369740565954 25.120530347305404 2 16.201481214514892 20.616496266516663 37.499481642426815 25.68254087654162 3 18.31669329981309 25.809950514562306 28.584008897707363 27.28934728791724 4 13.207668302954477 17.129730469216586 35.88283975359657 33.77976147423236 5 14.783792195167516 37.11192238950648 32.91090463143709 15.19338078388891 6 32.37881144362687 36.03813348289585 17.40626131862176 14.176793754855513 7 28.45065986746854 31.500241854277668 21.680847965482688 18.368250312771103 8 27.03003635873678 33.42314524082996 19.762573632607165 19.78424476782609 9 27.026867661279468 15.839257764307598 18.938505639523743 38.19536893488919 10-11 25.67648590900906 25.531814204664517 26.571084974408638 22.22061491191779 12-13 26.419352585512467 23.368606446811416 26.19147501902252 24.020565948653598 14-15 24.456103414161294 24.46233748198492 23.413229973177664 27.668329130676117 16-17 24.5132777378476 26.822142251646103 24.307966808031903 24.356613202474396 18-19 24.970603442610688 26.162584764379098 25.351790858252727 23.515020934757484 20-21 26.097474970098723 25.542054773675744 24.391274826087624 23.969195430137912 22-23 25.259161565408185 25.398170945165965 24.280261370698614 25.06240611872724 24-25 24.777154879046815 25.994105960447385 24.244514465058735 24.984224695447068 26-27 25.009197835117895 25.824806952233875 24.225199925907575 24.94079528674066 28-29 24.6536331465041 25.669364265066275 24.813617979531337 24.863384608898293 30-31 25.100728948939544 25.664835861634373 24.56965932670746 24.664775862718624 32-33 24.477193943458975 25.945245294843648 24.2588223640437 25.31873839765367 34-35 24.925804229524008 25.96138320589654 24.672514982434386 24.44029758214507 36-37 25.051481000972238 25.90126366276903 24.238475895098478 24.80877944116025 38-39 25.093418022972507 25.605493309019778 24.323252329027074 24.97783633898064 40-41 24.883677805189308 25.956447217835414 24.607670479711864 24.552204497263418 42-43 25.28518621539455 25.75115695343792 24.4551941357605 24.50846269540703 44-45 24.87501210649083 25.95736338470894 24.26558203530397 24.90204247349626 46-47 24.490401119900344 25.971147218648255 24.880302453549994 24.65814920790141 48-49 25.07842885757628 26.044959219473917 24.478483056184285 24.39812886676552 50-51 24.309978242070024 26.353912094546217 24.461786404166258 24.874323259217498 52-53 24.46188973125726 25.96856404137327 24.33832430736751 25.23122192000196 54-55 24.706678498307433 25.77894505244401 24.497048496087967 25.01732795316059 56-57 24.805204323811672 25.453285615505017 24.30195317133574 25.43955688934757 58-59 24.589615792677915 25.520723763563918 24.79837095886025 25.09128948489792 60-61 24.632744520461035 25.749682820272994 24.57903509855958 25.038537560706388 62-63 24.18566886312508 25.975383629379227 24.48850678989869 25.350440717597007 64-65 24.316074540438986 25.61590179131978 24.638393068102335 25.429630600138896 66-67 24.863623710761786 25.668307707865047 24.456170464385796 25.011898116987368 68-69 24.961062907875082 25.41127282030468 24.41940163143833 25.208262640381907 70-71 24.326689676920985 25.5706100830984 24.630526432240917 25.472173807739694 72-73 24.909509577939144 25.65279645127928 24.52595252767685 24.91174144310473 74-75 25.188885366583165 25.38978767384955 24.521419912618345 24.89990704694894 76-77 24.81828553353219 25.63198637515201 24.764514115376134 24.785213975939666 78-79 24.972876638612675 25.53591284594082 24.74296008419367 24.748250431252835 80-81 24.625056984890687 25.412133879396343 25.01568160817733 24.94712752753564 82-83 24.8322140253989 25.589064301550884 24.502945028747664 25.07577664430255 84-85 24.805149216029807 25.603536982763526 24.407415688882406 25.183898112324265 86-87 24.740576672627952 25.217810063590246 24.604722213382015 25.436891050399783 88-89 24.81510305912941 24.898074713201883 24.855931037019616 25.430891190649092 90-91 25.144447828980717 25.140142533522408 24.57650702906646 25.138902608430413 92-93 24.796538625113197 25.274219766803157 24.589994658678243 25.339246949405407 94-95 24.320703594115756 25.371299013033404 24.935086477197846 25.37291091565299 96-97 24.94026660869328 25.265085651958813 24.545619117330393 25.249028622017516 98-99 24.72425788072279 25.320778954007462 24.4553594591061 25.499603706163654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 997.0 1 994.5 2 1026.5 3 1048.5 4 948.0 5 805.0 6 711.5 7 692.5 8 706.0 9 688.5 10 658.0 11 654.5 12 680.5 13 702.5 14 742.5 15 861.5 16 1021.0 17 1196.5 18 1473.5 19 1961.5 20 2633.5 21 3653.0 22 5404.5 23 7582.5 24 10164.5 25 13819.5 26 18897.5 27 24422.0 28 30670.5 29 38509.0 30 46055.0 31 54782.5 32 64113.5 33 72776.0 34 81437.5 35 90165.5 36 98976.5 37 109375.0 38 121362.0 39 128668.5 40 136088.0 41 146788.0 42 163461.5 43 186583.0 44 206863.0 45 226136.0 46 258692.5 47 290917.0 48 314613.5 49 337934.0 50 358717.0 51 347000.0 52 325974.0 53 325852.0 54 317475.0 55 305300.0 56 281665.0 57 264292.5 58 252408.0 59 224634.0 60 191957.0 61 153188.0 62 125320.5 63 101300.0 64 76493.0 65 60224.5 66 44487.0 67 33503.5 68 26793.0 69 22911.5 70 20085.5 71 17543.0 72 16809.0 73 15135.5 74 11422.5 75 8491.0 76 6238.5 77 4312.0 78 2806.5 79 1903.0 80 1320.5 81 940.5 82 680.0 83 518.5 84 361.5 85 246.5 86 161.0 87 114.0 88 80.5 89 52.5 90 40.0 91 30.0 92 24.0 93 16.5 94 15.0 95 10.5 96 5.5 97 6.0 98 5.0 99 9.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002273196001985533 2 0.0 3 5.510778186631596E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.7530461859697515E-4 22-23 0.0 24-25 0.0044499533857050135 26-27 2.0665418199868482E-5 28-29 0.008645033280278316 30-31 5.304124004632912E-4 32-33 4.821930913302646E-5 34-35 4.1330836399736965E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.9287723653210585E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0665418199868482E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7258503.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.43885992924737 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63161915844822 33.60982930004462 2 12.92336827587079 11.485995052510022 3 4.532630872927685 6.042748454190469 4 2.144437496442544 3.8118543012574455 5 1.1653456167557747 2.5893315316086127 6 0.7071644584622218 1.8855349389926845 7 0.48796274366531506 1.5179155611503923 8 0.3623122428882267 1.2880594409889088 9 0.2681447544336634 1.0724442464736004 >10 1.536528449301516 12.603771791908185 >50 0.12514115498399583 3.8654372084107513 >100 0.09507266644463393 8.549176432692189 >500 0.011721868778953324 3.6208082372795465 >1k 0.008208230973156256 6.827837386741025 >5k 2.798260555624895E-4 0.7205893602733013 >10k+ 6.218356790277546E-5 0.5086667554783346 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19437 0.26778248903389584 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 17378 0.2394157583182097 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7650 0.10539363281932927 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.377694546657899E-5 3 0.0 0.0 0.0 0.0 1.377694546657899E-5 4 0.0 0.0 0.0 0.0 1.377694546657899E-5 5 0.0 1.377694546657899E-5 0.0 1.377694546657899E-5 1.377694546657899E-5 6 1.377694546657899E-5 1.377694546657899E-5 0.0 2.755389093315798E-5 1.377694546657899E-5 7 1.377694546657899E-5 1.377694546657899E-5 0.0 2.755389093315798E-5 1.377694546657899E-5 8 1.377694546657899E-5 1.377694546657899E-5 0.0 2.755389093315798E-5 1.377694546657899E-5 9 1.377694546657899E-5 1.377694546657899E-5 0.0 2.755389093315798E-5 1.377694546657899E-5 10-11 2.755389093315798E-5 1.377694546657899E-5 0.0 5.510778186631596E-5 1.377694546657899E-5 12-13 2.755389093315798E-5 1.377694546657899E-5 0.0 6.199625459960546E-5 1.377694546657899E-5 14-15 2.755389093315798E-5 1.377694546657899E-5 0.0 9.643861826605292E-5 1.377694546657899E-5 16-17 6.888472733289495E-5 1.377694546657899E-5 0.0 1.1021556373263192E-4 1.377694546657899E-5 18-19 7.577320006618444E-5 1.377694546657899E-5 0.0 1.1710403646592141E-4 5.510778186631596E-5 20-21 8.266167279947394E-5 1.377694546657899E-5 0.0 3.2375821846460627E-4 5.510778186631596E-5 22-23 1.1021556373263192E-4 1.377694546657899E-5 0.0 0.001391471492124478 5.510778186631596E-5 24-25 1.1021556373263192E-4 1.377694546657899E-5 0.0 0.0034029055302450106 5.510778186631596E-5 26-27 1.3088098193250042E-4 1.377694546657899E-5 0.0 0.005868978768762649 5.510778186631596E-5 28-29 1.377694546657899E-4 2.755389093315798E-5 0.0 0.015147751540503598 6.199625459960546E-5 30-31 1.377694546657899E-4 2.755389093315798E-5 0.0 0.041523713636269076 6.888472733289495E-5 32-33 1.446579273990794E-4 2.755389093315798E-5 0.0 0.0868911950577137 6.888472733289495E-5 34-35 1.6532334559894789E-4 2.755389093315798E-5 0.0 0.14502301645394375 9.643861826605292E-5 36-37 1.6532334559894789E-4 3.4442363666447474E-5 0.0 0.23300259020351716 9.643861826605292E-5 38-39 1.6532334559894789E-4 4.133083639973697E-5 0.0 0.37019341315971077 9.643861826605292E-5 40-41 1.6532334559894789E-4 4.133083639973697E-5 0.0 0.533574209447871 9.643861826605292E-5 42-43 1.7910029106552687E-4 4.133083639973697E-5 0.0 0.6780668135013515 1.0332709099934243E-4 44-45 1.9287723653210585E-4 4.821930913302646E-5 0.0 0.8433006089547666 1.1021556373263192E-4 46-47 2.0665418199868486E-4 5.510778186631596E-5 0.0 1.0244812187857468 1.2399250919921091E-4 48-49 2.0665418199868486E-4 5.510778186631596E-5 0.0 1.2156914449163967 1.515464001323689E-4 50-51 2.0665418199868486E-4 5.510778186631596E-5 0.0 1.4258863018999923 1.515464001323689E-4 52-53 2.0665418199868486E-4 5.510778186631596E-5 0.0 1.6338286282998022 1.791002910655269E-4 54-55 2.0665418199868486E-4 6.888472733289495E-5 0.0 1.8411785460445493 2.0665418199868486E-4 56-57 2.0665418199868486E-4 6.888472733289495E-5 0.0 2.075262626467193 2.0665418199868486E-4 58-59 2.0665418199868486E-4 9.643861826605292E-5 0.0 2.3282831184336494 2.0665418199868486E-4 60-61 2.0665418199868486E-4 9.643861826605292E-5 0.0 2.5877030015693316 2.0665418199868486E-4 62-63 2.0665418199868486E-4 9.643861826605292E-5 0.0 2.8496716196163314 2.0665418199868486E-4 64-65 2.0665418199868486E-4 9.643861826605292E-5 0.0 3.122806451963993 2.0665418199868486E-4 66-67 2.2043112746526384E-4 1.1021556373263192E-4 0.0 3.4155183238196636 2.0665418199868486E-4 68-69 2.2731960019855332E-4 1.1021556373263192E-4 0.0 3.7371135618460167 2.0665418199868486E-4 70-71 2.3420807293184282E-4 1.1021556373263192E-4 0.0 4.104792682458077 2.0665418199868486E-4 72-73 2.3420807293184282E-4 1.1021556373263192E-4 0.0 4.460361868005014 2.0665418199868486E-4 74-75 2.3420807293184282E-4 1.1021556373263192E-4 0.0 4.813072337367636 2.0665418199868486E-4 76-77 2.3420807293184282E-4 1.1021556373263192E-4 0.0 5.190106003951503 2.0665418199868486E-4 78-79 2.3420807293184282E-4 1.1021556373263192E-4 0.0 5.584023317204664 2.0665418199868486E-4 80-81 2.3420807293184282E-4 1.2399250919921091E-4 0.0 6.01416022008946 2.0665418199868486E-4 82-83 2.3420807293184282E-4 1.2399250919921091E-4 0.0 6.477217134166645 2.0665418199868486E-4 84-85 2.3420807293184282E-4 1.2399250919921091E-4 0.0 6.930361535980628 2.0665418199868486E-4 86-87 2.4798501839842183E-4 1.2399250919921091E-4 0.0 7.395588318968801 2.0665418199868486E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2010 0.0 20.126951 1 GTACATA 2355 0.0 18.758043 1 GTATTAG 3220 0.0 17.762499 1 GTACAAG 3820 0.0 17.6506 1 CATGGGG 15980 0.0 17.633629 4 TAGTACT 2630 0.0 17.326714 4 GTACATG 54545 0.0 17.092842 1 TACATGG 53535 0.0 16.633238 2 ACATGGG 50725 0.0 15.684612 3 GTATAAT 1730 0.0 15.320875 1 CTAGTAC 3040 0.0 15.295803 3 GTATCAA 39090 0.0 15.012343 1 CCTAGTA 3075 0.0 14.970487 2 GTATAAG 2440 0.0 14.864819 1 ATGGGTA 3965 0.0 14.776555 5 ATTGCGT 1045 0.0 14.68397 6 TACATGA 3310 0.0 14.469551 2 CTAACGC 710 0.0 14.408215 3 GTACTGT 4280 0.0 14.123599 6 GTATAAA 7510 0.0 14.117237 1 >>END_MODULE