FastQCFastQC Report
Fri 27 May 2016
ERR765273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7258503
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT188950.26031538459101No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188820.2601362842999445No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA187290.2580284116435579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117390.16172756283217077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96910.133512378516617No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG78120.10762549798491507No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72620.10004817797829661No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA475750.024.9582861
TATCACG5400.024.1112542
AGTACTT271350.021.35490212-13
CAACGCA562500.020.9065255
ATCAACG565950.020.7462143
AACGCAG579650.020.5200426
TCAACGC573200.020.5081464
TATCAAC591800.020.130742
GAGTACT259050.019.55928812-13
ACGCAGA620650.018.954727
GTACTAG19000.018.856021
CGCAGAG625250.018.807838
TACTTTT316300.018.45979714-15
GCAGAGT656600.017.8325489
GTACTTT290700.017.37377414-15
GTATAAG24050.017.2182031
ACTTTTT323850.015.89555616-17
GGTATCA350800.015.8629691
AGAGTAC629400.015.734549510-11
CATGGGG142200.015.7289954
Produced by FastQC (version 0.11.5)