FastQCFastQC Report
Fri 27 May 2016
ERR765272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12719992
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA769310.6048038394992701No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT624760.49116383092064836No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG372520.2928618194099493No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT311100.24457562551926135No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG303140.2383177599482767No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG273310.2148664873374134No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA236470.1859042049711981No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC230240.18100640314868122No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC217560.17103784342002729No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC197250.155070852245819No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT196340.15435544299084464No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC186650.14673751367139223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183980.14463845574745646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG56650.022.2651371
GTACTAG45100.020.1405471
TCTACAC88250.019.891033
CTACACT108100.019.2418884
TACACTC73650.019.2332085
TAGTACT54050.018.3712144
GTGATCG41700.018.2875258
GATCTAC69300.018.1329521
CGTTAAC14800.018.1260681
TCTAACG24150.017.733142
CATGGGG265950.017.6773284
TGATCGC43500.016.9908649
ATAAGGT53100.016.6619743
CTAGTAC59100.016.4037383
GTATAAA102850.016.3820861
TAGGCAT74500.015.9816725
CTAACGC25050.015.9688743
TAAGGTG57500.015.6320764
TATAAGG75050.015.488442
GTATATA34550.015.1204731
Produced by FastQC (version 0.11.5)