FastQCFastQC Report
Fri 27 May 2016
ERR765271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12843876
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT367060.28578600416260636No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA352720.2746211501886191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213460.1661959364914454No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG136450.10623740061022077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG169600.019.9228215
GTATCAA822900.018.8353441
GTACATG1197300.018.7565921
TACATGG1192050.018.4503422
CGCAGAT29550.018.4442218
CATGGGG283600.018.3790114
GTATAAT41600.017.9682391
GTATAAG47300.017.8901141
ACATGGG1125400.017.6747173
GTACTAG39550.017.2354741
GTATTAG55800.016.7656571
AACGCAG905300.015.9462856
TATCAAC969800.015.8978182
CAACGCA929800.015.4711125
TAGTACT48500.015.3113034
ATCAACG940750.015.2334033
GTACATA44650.015.161521
TCAACGC959800.014.9843364
ACGCAGA951650.014.9260337
CATGGGA856850.014.8758474
Produced by FastQC (version 0.11.5)