##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765271_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12843876
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.708996489844655	33.0	33.0	33.0	27.0	33.0
2	31.625414789118174	33.0	33.0	33.0	27.0	33.0
3	31.574201043361054	33.0	33.0	33.0	27.0	33.0
4	35.197845338899256	37.0	37.0	37.0	33.0	37.0
5	35.421291828105474	37.0	37.0	37.0	33.0	37.0
6	35.406473014843804	37.0	37.0	37.0	33.0	37.0
7	35.256218527802666	37.0	37.0	37.0	33.0	37.0
8	35.239795058750175	37.0	37.0	37.0	33.0	37.0
9	34.939498403752886	37.0	37.0	37.0	33.0	37.0
10-11	34.95105231473739	37.0	37.0	37.0	33.0	37.0
12-13	34.92305659911385	37.0	37.0	37.0	33.0	37.0
14-15	36.21440334677787	37.0	37.0	40.0	30.0	40.0
16-17	36.467102570906164	37.0	37.0	40.0	33.0	40.0
18-19	36.295844105003816	37.0	37.0	40.0	33.0	40.0
20-21	36.29763912389063	37.0	37.0	40.0	33.0	40.0
22-23	36.474846105645994	37.0	37.0	40.0	33.0	40.0
24-25	36.49026629500316	37.0	37.0	40.0	33.0	40.0
26-27	36.16045218748609	37.0	37.0	40.0	30.0	40.0
28-29	36.03896000708821	37.0	37.0	40.0	27.0	40.0
30-31	35.845423764601904	37.0	37.0	40.0	27.0	40.0
32-33	35.80581263008145	37.0	37.0	40.0	27.0	40.0
34-35	35.75994236475033	37.0	37.0	40.0	27.0	40.0
36-37	35.52425837029258	37.0	37.0	40.0	27.0	40.0
38-39	35.393616148271754	37.0	35.0	40.0	27.0	40.0
40-41	35.55198812258854	37.0	33.0	40.0	27.0	40.0
42-43	35.477620112495636	37.0	33.0	40.0	27.0	40.0
44-45	35.44142391284375	37.0	33.0	40.0	27.0	40.0
46-47	35.40922654500869	37.0	33.0	40.0	27.0	40.0
48-49	35.256461756560086	37.0	33.0	40.0	27.0	40.0
50-51	35.12809065581138	37.0	33.0	40.0	27.0	40.0
52-53	35.03170647240755	37.0	33.0	40.0	27.0	40.0
54-55	34.91047889282021	37.0	33.0	40.0	27.0	40.0
56-57	34.722308086748896	37.0	33.0	40.0	27.0	40.0
58-59	34.556874264435436	37.0	33.0	40.0	27.0	40.0
60-61	34.29443744240446	37.0	33.0	38.5	27.0	40.0
62-63	34.07943124022686	37.0	33.0	37.0	27.0	40.0
64-65	33.897829167768364	37.0	33.0	37.0	27.0	40.0
66-67	33.68509334721077	37.0	33.0	37.0	27.0	40.0
68-69	33.49088888743553	37.0	33.0	37.0	27.0	40.0
70-71	33.2574060587318	37.0	33.0	37.0	27.0	40.0
72-73	33.01913071256683	37.0	33.0	37.0	24.5	37.0
74-75	32.79460063301764	37.0	33.0	37.0	22.0	37.0
76-77	32.58323659462299	35.0	33.0	37.0	22.0	37.0
78-79	32.38967940830322	33.0	33.0	37.0	22.0	37.0
80-81	32.230857569786565	33.0	33.0	37.0	22.0	37.0
82-83	32.09130063230134	33.0	33.0	37.0	22.0	37.0
84-85	31.91705887693092	33.0	33.0	37.0	22.0	37.0
86-87	31.70156633402565	33.0	33.0	37.0	22.0	37.0
88-89	31.545559728231574	33.0	33.0	37.0	22.0	37.0
90-91	31.45805214874388	33.0	33.0	37.0	22.0	37.0
92-93	31.35578235884557	33.0	33.0	37.0	22.0	37.0
94-95	31.2128447829923	33.0	30.0	37.0	22.0	37.0
96-97	31.107981772791952	33.0	27.0	37.0	22.0	37.0
98-99	30.975141110051204	33.0	27.0	37.0	15.0	37.0
100	30.890130440374854	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	11.0
7	168.0
8	1206.0
9	2663.0
10	4468.0
11	7697.0
12	13276.0
13	21098.0
14	29350.0
15	34529.0
16	40331.0
17	47893.0
18	57564.0
19	69168.0
20	81849.0
21	97509.0
22	112299.0
23	120741.0
24	126729.0
25	139386.0
26	160800.0
27	193454.0
28	236004.0
29	294838.0
30	369071.0
31	458960.0
32	588757.0
33	777951.0
34	1034125.0
35	1416568.0
36	2108641.0
37	2933198.0
38	1262063.0
39	1510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30498036562959	19.064291307191354	13.047633484274277	24.583094842904785
2	15.39117023675872	21.409906522608647	38.23308011867218	24.965843121960457
3	17.439323733948026	27.092710126079538	29.807594364968732	25.6603717750037
4	12.045832473777814	17.728384193710443	37.68212745189429	32.54365588061746
5	13.515761643737692	38.125424245923675	33.548797246015646	14.81001686432299
6	30.52375147131044	37.56100379779939	17.9491540656141	13.966090665276077
7	26.895200348666155	32.62162127424392	22.465925011071086	18.01725336601884
8	26.928406642738338	32.8909263722393	20.50580246462744	19.67486452039492
9	27.1843313874767	14.540145964941567	20.130070344531394	38.145452303050334
10-11	24.8357576477897	25.727057837732602	27.330607902545058	22.106576611932645
12-13	25.940109559529272	23.675377096285125	27.094687065509344	23.289826278676262
14-15	23.87922917924185	24.520927085569777	25.052519419717978	26.547324315470394
16-17	23.215234607541504	27.078161323983522	25.74642881461332	23.960175253861657
18-19	23.703083979474783	26.128268770447306	27.762554233349835	22.406093016728075
20-21	25.253858944049572	25.23542832920208	26.691808973801738	22.8189037529466
22-23	24.210835019993453	25.572491679874542	26.413432342897647	23.803240957234355
24-25	23.538610327796828	26.13631235178558	26.56738387705116	23.75769344336643
26-27	23.961211426045427	25.71015203611353	26.694340489026118	23.634296048814917
28-29	23.44840532635408	25.596768979712653	27.34934043721593	23.605485256717337
30-31	23.988132398994725	25.652350016337817	26.913823971447965	23.445693613219493
32-33	23.4564306272528	25.763708949367302	26.442902334103653	24.33695808927624
34-35	23.90576948947477	25.75112464706168	27.110566685333215	23.232539178130338
36-37	24.028578337760496	25.707655385383777	26.59488639778133	23.668879879074392
38-39	24.114555499639316	25.269034156860116	26.59645889195476	24.019951451545808
40-41	23.744203399986493	25.8593426169297	26.849087068361726	23.547366914722083
42-43	24.45558795394742	25.42796168792566	26.525495406762552	23.590954951364367
44-45	23.92639657022769	25.807600069303437	26.23292499149288	24.033078368975993
46-47	23.566359777376846	25.669874806808085	26.78982698387199	23.97393843194308
48-49	24.124842446787177	26.07055937564909	26.125513094855407	23.67908508270833
50-51	23.453840106070125	26.38891187580321	25.818734717244663	24.338513300882
52-53	23.496075547558632	25.99519380122596	25.6636961093381	24.845034541877308
54-55	23.882129657827967	25.927337897350377	25.8830249483499	24.30750749647176
56-57	24.044350007739094	25.455859274879327	25.588907118069343	24.910883599312232
58-59	23.679580836812814	25.486153868193682	26.194927450249445	24.639337844744063
60-61	23.826000811593012	25.650298243302878	26.018146702755462	24.505554242348648
62-63	23.2336773828969	25.67761740713741	25.886699010704707	25.20200619926098
64-65	23.44916712463558	25.52332737341148	25.9990103433562	25.02849515859674
66-67	24.076842735957804	25.537226415528437	25.768832029015677	24.617098819498082
68-69	24.16646643870525	25.210321111667717	25.682665750211736	24.940546699415293
70-71	23.3794491230074	25.39681297659182	26.040415947987	25.183321952413777
72-73	24.00202135857065	25.411989423409842	25.761086815411936	24.82490240260757
74-75	24.305721274485126	25.24335151658741	25.775068244623174	24.675858964304293
76-77	23.75864216510007	25.545916534898115	26.042472904755492	24.65296839524632
78-79	23.78561469126781	25.681160307195466	25.867949737440988	24.665275264095737
80-81	23.38916996769081	25.52030764876701	26.28979160677265	24.800730776769537
82-83	23.58269709126332	25.726425051759243	25.572721025303917	25.118156831673517
84-85	23.59311826945656	25.84423181254481	25.325704735275412	25.23694518272322
86-87	23.620991945544322	25.342890151663205	25.687874776362445	25.348243126430027
88-89	23.51753783913516	25.102738619451138	25.8614522582019	25.518271283211803
90-91	23.894129502603782	25.227778693730773	25.653863590310383	25.22422821335506
92-93	23.60751072064964	25.466776643409574	25.55015850066511	25.375554135275674
94-95	23.099672452060926	25.58866227945445	25.939225817085866	25.37243945139876
96-97	23.6730883018932	25.352983997280187	25.541296631289722	25.43263106953689
98-99	23.395268616371983	25.392189117997514	25.616648118121745	25.59589414750876
100	23.306337277253732	25.64891091441643	25.31215641616163	25.73259539216821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2199.0
1	2101.0
2	2558.5
3	3072.0
4	2582.0
5	2265.0
6	2804.5
7	5079.5
8	8314.0
9	8290.0
10	5482.5
11	3715.5
12	3350.5
13	3422.5
14	3817.0
15	4436.0
16	4931.5
17	5712.0
18	6917.5
19	8215.5
20	10075.5
21	12699.5
22	16137.5
23	20455.5
24	25534.5
25	32376.5
26	42342.5
27	53418.0
28	65225.5
29	81448.0
30	99537.0
31	117026.0
32	138518.0
33	160146.0
34	180513.5
35	198764.5
36	217106.5
37	241240.0
38	256780.5
39	266559.0
40	276837.0
41	286420.5
42	300711.0
43	327795.0
44	362318.5
45	395955.5
46	442179.0
47	488306.5
48	536415.0
49	577965.5
50	632389.0
51	630149.5
52	564191.0
53	535357.0
54	510577.0
55	482555.0
56	460581.0
57	431463.0
58	403672.0
59	368931.5
60	316078.5
61	248512.0
62	190158.5
63	146513.0
64	110662.5
65	92670.5
66	77808.5
67	58125.5
68	43293.0
69	34910.0
70	29445.0
71	26231.0
72	23775.0
73	24857.5
74	20476.0
75	14616.0
76	10996.0
77	8590.5
78	6816.0
79	4529.5
80	3349.5
81	2719.0
82	2450.0
83	2277.5
84	1804.0
85	1273.5
86	881.0
87	465.5
88	218.5
89	141.0
90	121.5
91	96.5
92	55.0
93	35.0
94	29.5
95	21.5
96	14.0
97	7.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04814745953635803
2	0.02432287574249393
3	0.021613413271819194
4	0.022866928955091126
5	0.015361406478854202
6	0.025553033990673844
7	0.04968126444073425
8	0.006220863546175626
9	0.004033050459222746
10-11	0.0021761343694068676
12-13	0.0013508383294886996
14-15	0.0022851357331696446
16-17	0.0070578383036398054
18-19	0.005049098885725772
20-21	0.008673394230838105
22-23	0.018865021742657744
24-25	0.025813858682534774
26-27	0.02883086071525449
28-29	0.045360138948709876
30-31	0.04329689884891445
32-33	0.04098840568065278
34-35	0.04899611301136822
36-37	0.0459401819201618
38-39	0.05618241720801415
40-41	0.05313816483435374
42-43	0.05843251678854576
44-45	0.058954166172267626
46-47	0.06379694104801385
48-49	0.0664129737783205
50-51	0.03461182590053034
52-53	3.581473380776955E-4
54-55	3.892905848670604E-6
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.773656176686851E-4
64-65	2.141098216768832E-4
66-67	4.6325579599180186E-4
68-69	0.0014170177289160998
70-71	8.175102282208268E-5
72-73	3.036466561963071E-4
74-75	5.4500681881388454E-5
76-77	1.3235879885480052E-4
78-79	1.1678717546011812E-5
80-81	6.617939942740026E-5
82-83	2.763963152556129E-4
84-85	0.001463732599100147
86-87	0.004282196433537664
88-89	0.0028106780227401757
90-91	0.004231588657504946
92-93	0.0043172325861757
94-95	0.0032116473251532483
96-97	0.0026510688829446813
98-99	0.004286089339386335
100	0.0030753956204497774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2843876E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.56919994918223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43472272729737	33.620652403440374
2	13.413190793240371	11.956303648409204
3	4.777232276844945	6.387522616511681
4	2.1305618111939655	3.798297414687827
5	1.151829514080744	2.5668059960217042
6	0.6903579644813573	1.8461221293288033
7	0.4451289466482971	1.3887328718435772
8	0.32262252840943195	1.1503222381432556
9	0.23380014658918724	0.937825693333344
>10	1.2020197202673804	9.745860375494267
>50	0.09804685413292928	3.046564582051177
>100	0.07758695460763838	7.321652489129255
>500	0.01193043319469088	3.696985164193906
>1k	0.009972119859093614	8.669799005468539
>5k	8.158983975467912E-4	2.447656721871909
>10k+	1.8131075501039804E-4	1.4188966500712223
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	36706	0.28578600416260636	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	35272	0.2746211501886191	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21346	0.1661959364914454	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	13645	0.10623740061022077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.007230527607086E-5
2	0.0	0.0	0.0	0.0	7.007230527607086E-5
3	0.0	0.0	0.0	7.785811697341208E-6	7.007230527607086E-5
4	0.0	7.785811697341208E-6	0.0	1.5571623394682416E-5	7.007230527607086E-5
5	0.0	4.671487018404724E-5	0.0	3.114324678936483E-5	7.007230527607086E-5
6	0.0	4.671487018404724E-5	0.0	3.114324678936483E-5	1.5571623394682415E-4
7	0.0	5.4500681881388454E-5	0.0	3.114324678936483E-5	1.6350204564416536E-4
8	0.0	5.4500681881388454E-5	0.0	3.114324678936483E-5	1.6350204564416536E-4
9	0.0	5.4500681881388454E-5	0.0	3.892905848670604E-5	3.192182795909895E-4
10-11	0.0	5.4500681881388454E-5	7.785811697341208E-6	6.228649357872967E-5	3.7371896147237794E-4
12-13	0.0	6.617939942740026E-5	7.785811697341208E-6	6.228649357872967E-5	4.282196433537664E-4
14-15	0.0	7.785811697341208E-5	7.785811697341208E-6	6.228649357872967E-5	5.138635720245197E-4
16-17	7.785811697341208E-6	7.785811697341208E-5	7.785811697341208E-6	8.175102282208268E-5	5.294351954192021E-4
18-19	1.5571623394682416E-5	8.564392867075329E-5	7.785811697341208E-6	1.0900136376277691E-4	5.411139129652139E-4
20-21	1.5571623394682416E-5	1.4014461055214173E-4	7.785811697341208E-6	2.024311041308714E-4	5.488997246625552E-4
22-23	1.946452924335302E-5	1.4014461055214173E-4	7.785811697341208E-6	8.136173223721561E-4	5.566855363598963E-4
24-25	3.114324678936483E-5	1.4014461055214173E-4	7.785811697341208E-6	0.0026354972595499988	5.644713480572375E-4
26-27	6.228649357872967E-5	1.4014461055214173E-4	7.785811697341208E-6	0.004924525898568314	5.839358773005905E-4
28-29	7.007230527607086E-5	1.4014461055214173E-4	7.785811697341208E-6	0.014870900341921706	5.839358773005905E-4
30-31	7.396521112474147E-5	1.4014461055214173E-4	7.785811697341208E-6	0.04882871805987538	5.839358773005905E-4
32-33	7.785811697341208E-5	1.4014461055214173E-4	7.785811697341208E-6	0.11433075186960696	6.851514293660263E-4
34-35	7.785811697341208E-5	1.4403751640081232E-4	7.785811697341208E-6	0.19774404548907198	7.318662995500735E-4
36-37	8.175102282208268E-5	1.6350204564416536E-4	7.785811697341208E-6	0.32326690167360694	8.175102282208268E-4
38-39	8.564392867075329E-5	1.7907366903884779E-4	7.785811697341208E-6	0.5280220705961347	8.875825334968976E-4
40-41	9.342974036809448E-5	1.7907366903884779E-4	7.785811697341208E-6	0.7843076342375153	9.031541568915801E-4
42-43	1.051084579141063E-4	1.7907366903884779E-4	7.785811697341208E-6	1.0135219306072405	9.576548387729686E-4
44-45	1.1289426961144751E-4	1.8296657488751838E-4	7.785811697341208E-6	1.2750200951799908	9.693335563189803E-4
46-47	1.1678717546011812E-4	1.9853819828220077E-4	7.785811697341208E-6	1.5658396266049284	9.771193680163217E-4
48-49	1.2068008130878873E-4	2.024311041308714E-4	7.785811697341208E-6	1.8700273967142005	9.887980855623334E-4
50-51	1.3235879885480052E-4	2.024311041308714E-4	7.785811697341208E-6	2.207234794231897	9.965838972596746E-4
52-53	1.3625170470347114E-4	2.024311041308714E-4	7.785811697341208E-6	2.5458981385369963	9.965838972596746E-4
54-55	1.4014461055214173E-4	2.530388801635893E-4	7.785811697341208E-6	2.87863258723457	9.965838972596746E-4
56-57	1.4014461055214173E-4	2.5693178601225985E-4	7.785811697341208E-6	3.2503505951007314	0.0010121555206543571
58-59	1.4014461055214173E-4	2.958608444989659E-4	7.785811697341208E-6	3.6565675345978113	0.0010471916732923924
60-61	1.4014461055214173E-4	2.958608444989659E-4	7.785811697341208E-6	4.075420846479677	0.0010666562025357454
62-63	1.4014461055214173E-4	2.958608444989659E-4	7.785811697341208E-6	4.502721764053156	0.0010666562025357454
64-65	1.4014461055214173E-4	3.114324678936483E-4	7.785811697341208E-6	4.9417870431013196	0.0010822278259304278
66-67	1.4014461055214173E-4	3.347899029856719E-4	7.785811697341208E-6	5.412474396358233	0.0010822278259304278
68-69	1.4403751640081232E-4	3.347899029856719E-4	7.785811697341208E-6	5.927914595251465	0.0010939065434764396
70-71	1.4793042224948294E-4	3.464686205316837E-4	7.785811697341208E-6	6.516307849748783	0.0011367285078118163
72-73	1.4793042224948294E-4	3.5814733807769557E-4	7.785811697341208E-6	7.08587501156193	0.0011756575662985223
74-75	1.5182332809815356E-4	3.5814733807769557E-4	7.785811697341208E-6	7.643856885569434	0.001187336283844534
76-77	1.5571623394682415E-4	3.5814733807769557E-4	7.785811697341208E-6	8.22897620624802	0.0012535156832719344
78-79	1.5571623394682415E-4	3.6593314977503675E-4	7.785811697341208E-6	8.837939575249715	0.001296337647607311
80-81	1.5571623394682415E-4	3.815047731697192E-4	7.785811697341208E-6	9.507087268671857	0.0013002305534559817
82-83	1.5571623394682415E-4	3.815047731697192E-4	7.785811697341208E-6	10.220022367079846	0.0013313738002453464
84-85	1.5571623394682415E-4	3.815047731697192E-4	7.785811697341208E-6	10.908587874875156	0.0013625170470347114
86-87	1.5571623394682415E-4	3.853976790183898E-4	7.785811697341208E-6	11.608941101580239	0.0013703028587320526
88	1.5571623394682415E-4	3.8929058486706036E-4	7.785811697341208E-6	12.154181494745044	0.0013703028587320526
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAG	16960	0.0	19.922821	5
GTATCAA	82290	0.0	18.835344	1
GTACATG	119730	0.0	18.756592	1
TACATGG	119205	0.0	18.450342	2
CGCAGAT	2955	0.0	18.444221	8
CATGGGG	28360	0.0	18.379011	4
GTATAAT	4160	0.0	17.968239	1
GTATAAG	4730	0.0	17.890114	1
ACATGGG	112540	0.0	17.674717	3
GTACTAG	3955	0.0	17.235474	1
GTATTAG	5580	0.0	16.765657	1
AACGCAG	90530	0.0	15.946285	6
TATCAAC	96980	0.0	15.897818	2
CAACGCA	92980	0.0	15.471112	5
TAGTACT	4850	0.0	15.311303	4
ATCAACG	94075	0.0	15.233403	3
GTACATA	4465	0.0	15.16152	1
TCAACGC	95980	0.0	14.984336	4
ACGCAGA	95165	0.0	14.926033	7
CATGGGA	85685	0.0	14.875847	4
>>END_MODULE