FastQCFastQC Report
Fri 27 May 2016
ERR765268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13575322
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182750.134619274592529No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA304100.036.8339961
CAACGCA367600.028.1320295
ATCAACG368900.028.0328883
AACGCAG390100.026.7141026
TCAACGC389400.026.6415214
TATCAAC418250.026.6340962
GGTATCA182250.023.7338891
ACGCAGA434200.023.6114777
GTACATG608000.023.4362871
CGCAGAG446000.022.849898
TACATGG601350.022.6636222
GCAGAGT452250.021.4958299
ACATGGG625800.020.5849343
AGAGTAC415050.019.41232310-11
CATGGGG310550.018.646224
GTATAAG40700.018.0014291
AGTACAT457000.017.55039612-13
GTATAAA91000.016.9281431
GTACAAG70100.016.1464061
GTATTAT42900.016.0929931
Produced by FastQC (version 0.11.5)