FastQCFastQC Report
Fri 27 May 2016
ERR765267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10708904
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA856100.7994282141291023No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT689290.6436606397816247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG379410.3542939594938941No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT340820.31825852580245373No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG308160.28776054020093933No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG303310.2832315986771382No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA238130.2223663598067552No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC235560.21996648770032864No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC229450.21426095518271523No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC212100.19805948395839576No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC195510.18256770253986776No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT195430.18249299834978444No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG126520.11814467661676675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119460.11155203184191398No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC118850.11098241239252868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC58900.023.0581461
TCTACAC79350.021.734663
TACACTC65750.021.7335765
CTACACT92650.019.3764384
ATCTACA77700.018.3250482
CATGGGG207550.018.2275874
GTATAAA61250.017.8768771
GTATAAG35200.016.8216931
GTACTAG24450.016.3373581
GTATTAG32800.015.1870631
CACTCTT96100.015.1148087
GTACTAA12500.015.0380581
TCTAACG13150.015.0088382
TAGTACT35650.014.632534
TATACCG16000.014.395595
TTATACT35700.014.0854764
ACACTCT107900.013.7666796
CTAGTAC30950.013.51335053
ACTCTTT122400.013.4032958
CTAACGC13000.013.3749473
Produced by FastQC (version 0.11.5)