FastQCFastQC Report
Fri 27 May 2016
ERR765266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11755466
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT501950.42699285591911035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA492440.4189030022289206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236350.20105540690602994No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG192350.16362601023217624No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG158660.13496700173349147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152950.13010968684695273No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC142930.12158599242258877No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG141520.120386550392813No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC138880.1181407865923818No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA138030.11741771870209143No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT135210.11501883464253991No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA133680.11371731244001727No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC125340.10662274043410955No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC123470.10503199107547076No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA121470.10333065486302286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA37700.033.268982
GTACATA27750.030.3019581
GTACAAG58250.027.1778221
GTACTAG58500.025.2950381
GTACAAA57450.023.3860381
TAGGCAT76450.023.1628915
ACGTTAA13000.022.039828
TAGTACT71450.021.695254
AGGGCTA54150.021.4253255
CGTTAAC18200.021.1652371
CTATTGA54900.021.1322699
GTATAAG55050.020.9922241
CTAGGCA88550.020.8476414
GTGATCG42500.020.7773658
CTGTGCG78400.020.0701359
TACATAA31650.020.0368582
GGGCTAT60350.019.9243536
CTAACGC27500.019.8149533
CATGGGG345650.019.5151024
TACAAGA44550.019.507142
Produced by FastQC (version 0.11.5)