##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765266_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11755466
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.755734566371082	33.0	33.0	33.0	27.0	33.0
2	31.614774437695623	33.0	33.0	33.0	27.0	33.0
3	31.652543931478345	33.0	33.0	33.0	27.0	33.0
4	35.34118630431154	37.0	37.0	37.0	33.0	37.0
5	35.456622221526565	37.0	37.0	37.0	33.0	37.0
6	35.42713159988723	37.0	37.0	37.0	33.0	37.0
7	35.23276729310433	37.0	37.0	37.0	33.0	37.0
8	35.171903946640654	37.0	37.0	37.0	33.0	37.0
9	34.9830120728519	37.0	37.0	37.0	33.0	37.0
10-11	35.0095386265419	37.0	37.0	37.0	33.0	37.0
12-13	34.95733240179504	37.0	37.0	37.0	33.0	37.0
14-15	36.52524919046169	40.0	37.0	40.0	33.0	40.0
16-17	36.60489337470756	40.0	37.0	40.0	33.0	40.0
18-19	36.582395500101825	40.0	37.0	40.0	33.0	40.0
20-21	36.31200566613012	40.0	37.0	40.0	27.0	40.0
22-23	36.38328633675603	38.5	37.0	40.0	33.0	40.0
24-25	36.29041307252303	37.0	37.0	40.0	27.0	40.0
26-27	36.2650891508682	37.0	37.0	40.0	27.0	40.0
28-29	36.18421983441575	37.0	37.0	40.0	27.0	40.0
30-31	36.27771298049775	37.0	37.0	40.0	33.0	40.0
32-33	36.01634903286693	37.0	37.0	40.0	27.0	40.0
34-35	35.81460513772912	37.0	37.0	40.0	27.0	40.0
36-37	35.88187316436456	37.0	37.0	40.0	27.0	40.0
38-39	35.75242244756609	37.0	37.0	40.0	27.0	40.0
40-41	35.738441504573274	37.0	37.0	40.0	27.0	40.0
42-43	35.59836862273261	37.0	37.0	40.0	27.0	40.0
44-45	35.445817375508554	37.0	35.0	40.0	27.0	40.0
46-47	35.178736342736215	37.0	33.0	40.0	27.0	40.0
48-49	34.91187627100449	37.0	33.0	40.0	27.0	40.0
50-51	34.66926538684217	37.0	33.0	40.0	24.5	40.0
52-53	34.5044982053455	37.0	33.0	40.0	24.5	40.0
54-55	34.34302851116239	37.0	33.0	40.0	24.5	40.0
56-57	34.17017628225032	37.0	33.0	40.0	22.0	40.0
58-59	33.98397141380869	37.0	33.0	37.0	22.0	40.0
60-61	33.749947216044006	37.0	33.0	37.0	22.0	40.0
62-63	33.50985209773904	37.0	33.0	37.0	22.0	40.0
64-65	33.32529531368641	37.0	33.0	37.0	22.0	40.0
66-67	33.07601655264028	37.0	33.0	37.0	22.0	40.0
68-69	32.868948198225404	37.0	33.0	37.0	22.0	40.0
70-71	32.60133779469057	37.0	33.0	37.0	22.0	37.0
72-73	32.40982373646438	37.0	33.0	37.0	22.0	37.0
74-75	32.22177917064283	37.0	33.0	37.0	22.0	37.0
76-77	31.980732282327217	33.0	33.0	37.0	22.0	37.0
78-79	31.903211365674487	33.0	33.0	37.0	22.0	37.0
80-81	31.766647106971345	33.0	33.0	37.0	22.0	37.0
82-83	31.63394709320753	33.0	33.0	37.0	22.0	37.0
84-85	31.47910316783699	33.0	33.0	37.0	22.0	37.0
86-87	31.315099290832023	33.0	33.0	37.0	15.0	37.0
88-89	31.19008578647584	33.0	33.0	37.0	15.0	37.0
90-91	31.075296844889007	33.0	33.0	37.0	15.0	37.0
92-93	30.950751207991246	33.0	27.0	37.0	15.0	37.0
94-95	30.823297264438516	33.0	27.0	37.0	15.0	37.0
96-97	30.705397387053818	33.0	27.0	37.0	15.0	37.0
98-99	30.593234840711546	33.0	27.0	37.0	15.0	37.0
100	30.367146142909178	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	19.0
7	302.0
8	1538.0
9	2957.0
10	4708.0
11	8910.0
12	17681.0
13	33433.0
14	50401.0
15	56416.0
16	60694.0
17	68870.0
18	79104.0
19	90134.0
20	101971.0
21	119163.0
22	134333.0
23	129100.0
24	118033.0
25	118964.0
26	131376.0
27	152827.0
28	184393.0
29	229026.0
30	284788.0
31	357587.0
32	467958.0
33	625769.0
34	845337.0
35	1220178.0
36	2042672.0
37	2976652.0
38	1039088.0
39	1082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.547673096771646	19.49128640187942	13.004173852169382	23.956866649179545
2	15.719001832779462	21.660673098976215	37.52726540184694	25.093059666397387
3	17.626049905431092	28.08223513389719	29.381706854765437	24.910008105906286
4	12.433014707796522	17.955520830160776	36.994350903479415	32.61711355856329
5	13.35825760503393	38.528751569146856	32.77532369476845	15.337667131050756
6	30.050392139248228	38.11760060052703	17.61353969303977	14.21846756718498
7	26.173162082658163	33.1639579885159	22.51971394234942	18.14316598647652
8	27.282667794408916	32.37151110059595	19.938297458011466	20.40752364698367
9	27.49634379302296	14.48680566945167	19.92510305093374	38.091747486591636
10-11	25.38226645685185	25.82596295478551	25.88006979403715	22.91170079432549
12-13	26.410118120735714	24.235992223112124	26.136361799674958	23.2175278564772
14-15	24.610616598949633	24.46697638215588	24.46499856980774	26.45740844908675
16-17	23.24629330727316	26.628865747857276	25.62437060615253	24.500470338717033
18-19	23.787804017189767	25.499928414251034	28.264890750279047	22.44737681828015
20-21	25.614988386188752	24.578752367818485	26.9625069583546	22.843752287638164
22-23	24.598791308608003	24.564479443359964	26.693652768602966	24.14307647942907
24-25	23.707836397238143	25.50149667386192	26.835516986900547	23.955149941999395
26-27	24.273144251972376	25.03824011529236	26.77929545100362	23.909320181731648
28-29	23.356769813644306	24.943222013065604	27.82922733646857	23.870780836821517
30-31	24.089157659961383	25.124627385773472	27.23063916476548	23.55557578949966
32-33	23.45822442204597	25.348382446929165	26.54799826964495	24.645394861379916
34-35	23.9631287578217	25.454230025825424	27.418453484551787	23.164187731801086
36-37	24.167297484176373	25.32715068347428	26.590360087229403	23.91519174511994
38-39	24.338070745871203	24.72209349974915	26.630266929983907	24.30956882439574
40-41	23.77500957406068	25.37268201353134	27.129062593081144	23.723245819326838
42-43	24.728398177136416	25.07678742634352	26.508605153602517	23.686209242917553
44-45	24.017648511082935	25.623522769717898	26.106023316718087	24.25280540248108
46-47	23.52547264840038	25.516230741132983	26.673532949134703	24.284763661331937
48-49	24.56742850575008	25.83541183000318	25.763398319395385	23.833761344851354
50-51	23.47142700118781	26.32653322627327	25.310617240366966	24.891422532171955
52-53	23.410567842235245	25.972604884590734	25.002783675937145	25.614043597236876
54-55	23.883896367471777	25.922857251167287	25.112967263819208	25.080279117541732
56-57	24.39226685629334	25.101222738949712	24.73624527697059	25.770265127786356
58-59	23.669695014287484	25.233490434396693	25.872428218836063	25.22438633247976
60-61	23.898674572130552	25.568719983963085	25.449089772348643	25.08351567155772
62-63	23.300143521975507	25.530175350704816	25.127862016060032	26.041819111259652
64-65	23.23164527514963	25.35535484516542	25.463965982323046	25.9490338973619
66-67	24.435706682345852	25.18963931346263	25.161963903266678	25.21269010092484
68-69	24.393753993141953	24.647753665798444	25.21523133515521	25.743261005904394
70-71	23.345527005798562	25.016585286407782	25.54946299750594	26.08842471028771
72-73	24.329928258670748	24.97552619426854	25.44219838039352	25.252347166667192
74-75	24.74162174451226	24.716496082166447	25.46387605331235	25.078006120008943
76-77	23.916384327509224	25.15696349703133	25.675469737536478	25.251182437922964
78-79	24.031877436965168	25.323293214466208	25.489547903222398	25.15528144534623
80-81	23.487904713984157	25.041453683190863	26.088134986934143	25.382506615890843
82-83	23.848389959754044	25.034041030817512	25.409212437857704	25.708356571570746
84-85	23.93994159984333	25.30872851023696	24.806524432521943	25.944805457397763
86-87	23.71723508302217	24.69243381963078	25.32592497477634	26.264406122570715
88-89	23.812611253277236	24.135142050635043	25.58253766773563	26.469709028352096
90-91	24.210093194650042	24.40263638880442	25.22264787885304	26.164622537692495
92-93	23.863819123956333	24.533068075971915	25.242405165727988	26.360707634343765
94-95	22.909909409318736	24.799093693251056	25.928662763571857	26.362334133858344
96-97	23.859010238000266	24.63053742853344	25.125688605238665	26.38476372822763
98-99	23.358144403149392	24.759015357646593	25.195373346750088	26.687466892453926
100	23.246266344953234	25.00874862643161	24.861128320903703	26.883856707711455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1503.0
1	1243.0
2	1213.0
3	1448.0
4	1200.5
5	1012.5
6	1289.0
7	2366.0
8	3929.0
9	3973.0
10	2582.0
11	1776.0
12	1752.5
13	1932.5
14	2266.5
15	2873.0
16	3563.0
17	4340.0
18	5524.5
19	6965.0
20	8695.0
21	10899.5
22	13713.5
23	17397.5
24	21702.0
25	26623.0
26	33503.5
27	41343.5
28	50399.5
29	65112.5
30	81545.0
31	95570.0
32	112836.0
33	129742.0
34	145596.0
35	158657.0
36	170190.0
37	190715.5
38	203717.0
39	210759.5
40	211670.5
41	209077.0
42	218655.5
43	249983.0
44	298156.0
45	350846.5
46	417766.0
47	480676.5
48	541142.0
49	585740.5
50	643141.0
51	642817.0
52	568546.5
53	538332.5
54	513116.5
55	478115.5
56	455799.5
57	418292.5
58	384696.0
59	356655.0
60	302852.0
61	233772.0
62	179192.5
63	137638.5
64	100155.5
65	80202.0
66	63503.5
67	43527.0
68	31864.5
69	26269.5
70	23448.0
71	22400.0
72	20804.5
73	23028.5
74	18635.5
75	12938.5
76	9307.0
77	6382.5
78	4723.5
79	2696.5
80	1729.0
81	1366.5
82	1201.0
83	1105.0
84	889.5
85	649.5
86	470.0
87	259.5
88	131.5
89	90.0
90	87.0
91	69.0
92	45.0
93	27.5
94	16.0
95	14.5
96	11.5
97	6.0
98	3.5
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017481229582902116
2	0.0054612892419577405
3	0.009136175460845193
4	0.008676814683484262
5	0.019259125924910168
6	0.003896059926505678
7	0.024414174648627288
8	0.008566227829675149
9	9.697616410952999E-4
10-11	1.743869617759092E-4
12-13	0.001029308408530976
14-15	4.551074368298118E-4
16-17	9.187215547218631E-4
18-19	0.0028327247937257444
20-21	0.006052503575783384
22-23	0.012883368468761681
24-25	0.021874930351548805
26-27	0.02622184437435317
28-29	0.03209145430729841
30-31	0.034332964767198514
32-33	0.03505177931695774
34-35	0.04444315520967012
36-37	0.04118084302230129
38-39	0.045774450795910596
40-41	0.04224417815508122
42-43	0.04951739046329597
44-45	0.04264399216500647
46-47	0.04503436954349577
48-49	0.04753108043526305
50-51	0.048279668368740127
52-53	0.03333342974238537
54-55	0.029769130377307034
56-57	0.021679276687117294
58-59	0.02145384963896795
60-61	0.021964250502702315
62-63	0.021794116881457527
64-65	0.02541796301397154
66-67	0.026847085432427774
68-69	0.027948700629987784
70-71	0.028348514639913044
72-73	0.02641324469825356
74-75	0.02162398326021274
76-77	0.025962390601954866
78-79	0.025651896743183127
80-81	0.02459706829146543
82-83	0.02640899135772244
84-85	0.027829607095116435
86-87	0.030666585229373294
88-89	0.03032631798688372
90-91	0.030262517878916925
92-93	0.03283578890024436
94-95	0.0305815184187509
96-97	0.03320582952645178
98-99	0.03395016411939773
100	0.042958739364309335
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1755466E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.66872801563963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.45748019233018	18.790113759165703
2	13.74751608512467	7.332575347296415
3	5.7897162553574395	4.632131043055654
4	2.996547657688895	3.196564578752285
5	1.7873767665789488	2.3833532424683694
6	1.1200493451032858	1.7922174809192892
7	0.7398479943753544	1.3811563454438964
8	0.515705208662142	1.1002561556847477
9	0.380732893201782	0.9138295777865304
>10	2.0251651214088664	9.79424277926661
>50	0.18207253729889844	3.3890842384713875
>100	0.17867482433545365	10.41140331563804
>500	0.03851307600708706	7.274130170282776
>1k	0.03739839300592846	19.849800020133817
>5k	0.002495474363787951	4.5413050174645
>10k+	7.081751572911752E-4	3.217836928170149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	50195	0.42699285591911035	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	49244	0.4189030022289206	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23635	0.20105540690602994	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	19235	0.16362601023217624	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	15866	0.13496700173349147	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15295	0.13010968684695273	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	14293	0.12158599242258877	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	14152	0.120386550392813	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	13888	0.1181407865923818	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	13803	0.11741771870209143	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	13521	0.11501883464253991	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	13368	0.11371731244001727	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	12534	0.10662274043410955	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	12347	0.10503199107547076	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	12147	0.10333065486302286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.104008637343683E-5	0.0
6	0.0	0.0	0.0	5.104008637343683E-5	2.5520043186718415E-5
7	0.0	0.0	0.0	5.104008637343683E-5	3.402672424895789E-5
8	8.506681062239472E-6	0.0	0.0	5.104008637343683E-5	3.402672424895789E-5
9	3.402672424895789E-5	0.0	0.0	1.190935348713526E-4	5.104008637343683E-5
10-11	5.95467674356763E-5	0.0	0.0	1.6162694018254998E-4	6.380010796679605E-5
12-13	7.656012956015525E-5	0.0	0.0	1.7013362124478944E-4	8.081347009127499E-5
14-15	1.0208017274687366E-4	0.0	0.0	1.914003239003881E-4	1.1058685380911313E-4
16-17	1.403602375269513E-4	0.0	0.0	2.381870697427052E-4	1.1058685380911313E-4
18-19	1.5737359965143022E-4	0.0	0.0	2.5945377239830394E-4	1.1058685380911313E-4
20-21	1.7013362124478944E-4	0.0	0.0	4.8488082054764993E-4	1.1484019434023288E-4
22-23	2.1266702655598678E-4	4.253340531119736E-6	0.0	0.0023946307190204114	1.190935348713526E-4
24-25	2.594537723983039E-4	8.506681062239472E-6	0.0	0.006579917801642232	1.190935348713526E-4
26-27	3.1474719930286044E-4	8.506681062239472E-6	0.0	0.010522764473990228	1.2334687540247233E-4
28-29	3.87053988331896E-4	8.506681062239472E-6	0.0	0.025222309349540034	1.276002159335921E-4
30-31	3.9130732886301573E-4	8.506681062239472E-6	0.0	0.07428884571653732	1.276002159335921E-4
32-33	4.338407341742131E-4	8.506681062239472E-6	0.0	0.15802010741216044	1.4461357805807103E-4
34-35	4.7212079895429067E-4	8.506681062239472E-6	0.0	0.2643833940738717	1.7013362124478944E-4
36-37	4.8488082054764993E-4	8.506681062239472E-6	0.0	0.4317863706976823	1.914003239003881E-4
38-39	4.891341610787697E-4	8.506681062239472E-6	0.0	0.6938474408415626	2.0841368602486708E-4
40-41	5.104008637343684E-4	8.506681062239472E-6	0.0	1.022124516373915	2.2117370761822627E-4
42-43	5.359209069210867E-4	1.2760021593359208E-5	0.0	1.2965925808470715	2.25427048149346E-4
44-45	5.742009717011644E-4	1.7013362124478944E-5	0.0	1.6143809186296827	2.3393372921158548E-4
46-47	5.784543122322841E-4	1.7013362124478944E-5	0.0	1.9545886143518258	2.4669375080494467E-4
48-49	5.827076527634039E-4	1.7013362124478944E-5	0.0	2.3198952725481066	2.4669375080494467E-4
50-51	5.869609932945235E-4	1.7013362124478944E-5	0.0	2.7359825633454258	2.4669375080494467E-4
52-53	6.039743554190026E-4	1.7013362124478944E-5	8.506681062239472E-6	3.144962522115244	2.594537723983039E-4
54-55	6.209877175434815E-4	1.7013362124478944E-5	8.506681062239472E-6	3.5370056788901434	2.722137939916631E-4
56-57	6.252410580746012E-4	1.7013362124478944E-5	8.506681062239472E-6	3.9819433785100475	2.7646713452278285E-4
58-59	6.29494398605721E-4	1.7013362124478944E-5	8.506681062239472E-6	4.462664432018263	2.807204750539026E-4
60-61	6.29494398605721E-4	1.7013362124478944E-5	8.506681062239472E-6	4.930702024062679	2.807204750539026E-4
62-63	6.337477391368408E-4	1.7013362124478944E-5	8.506681062239472E-6	5.4028270763575	2.8922715611614207E-4
64-65	6.380010796679604E-4	2.5520043186718415E-5	8.506681062239472E-6	5.897588406958942	2.977338371783815E-4
66-67	6.380010796679604E-4	2.5520043186718415E-5	8.506681062239472E-6	6.429685560742552	3.019871777095013E-4
68-69	6.380010796679604E-4	2.5520043186718415E-5	8.506681062239472E-6	6.995486184894755	3.1474719930286044E-4
70-71	6.380010796679604E-4	2.5520043186718415E-5	8.506681062239472E-6	7.646179232707576	3.360139019584591E-4
72-73	6.507611012613196E-4	2.5520043186718415E-5	8.506681062239472E-6	8.255266103444985	3.4026724248957887E-4
74-75	6.635211228546788E-4	2.5520043186718415E-5	8.506681062239472E-6	8.84300120471617	3.4026724248957887E-4
76-77	6.635211228546788E-4	2.5520043186718415E-5	8.506681062239472E-6	9.483971116074855	3.6153394514517755E-4
78-79	6.677744633857986E-4	2.5520043186718415E-5	8.506681062239472E-6	10.138164663144789	3.7429396673853676E-4
80-81	6.720278039169183E-4	2.5520043186718415E-5	8.506681062239472E-6	10.840429464897436	3.785473072696565E-4
82-83	6.890411660413973E-4	2.5520043186718415E-5	8.506681062239472E-6	11.590642174457397	4.040673504563749E-4
84-85	6.890411660413973E-4	3.402672424895789E-5	8.506681062239472E-6	12.311949181767869	4.0832069098749465E-4
86-87	6.93294506572517E-4	3.828006478007762E-5	8.506681062239472E-6	13.040333747722123	4.0832069098749465E-4
88	6.975478471036368E-4	4.253340531119736E-5	8.506681062239472E-6	13.611497834284068	4.1682737204973416E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGA	3770	0.0	33.26898	2
GTACATA	2775	0.0	30.301958	1
GTACAAG	5825	0.0	27.177822	1
GTACTAG	5850	0.0	25.295038	1
GTACAAA	5745	0.0	23.386038	1
TAGGCAT	7645	0.0	23.162891	5
ACGTTAA	1300	0.0	22.03982	8
TAGTACT	7145	0.0	21.69525	4
AGGGCTA	5415	0.0	21.425325	5
CGTTAAC	1820	0.0	21.165237	1
CTATTGA	5490	0.0	21.132269	9
GTATAAG	5505	0.0	20.992224	1
CTAGGCA	8855	0.0	20.847641	4
GTGATCG	4250	0.0	20.777365	8
CTGTGCG	7840	0.0	20.070135	9
TACATAA	3165	0.0	20.036858	2
GGGCTAT	6035	0.0	19.924353	6
CTAACGC	2750	0.0	19.814953	3
CATGGGG	34565	0.0	19.515102	4
TACAAGA	4455	0.0	19.50714	2
>>END_MODULE