##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765265_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6947830
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3340985602699	34.0	31.0	34.0	31.0	34.0
2	32.370628958969924	34.0	31.0	34.0	31.0	34.0
3	32.40286204469597	34.0	31.0	34.0	31.0	34.0
4	35.854391658978415	37.0	35.0	37.0	35.0	37.0
5	35.86426193502144	37.0	37.0	37.0	35.0	37.0
6	35.96010006001874	37.0	37.0	37.0	35.0	37.0
7	35.91527743194638	37.0	37.0	37.0	35.0	37.0
8	35.94427137681837	37.0	37.0	37.0	35.0	37.0
9	37.66094593563746	39.0	38.0	39.0	35.0	39.0
10-11	37.59331107698375	39.0	38.0	39.0	35.0	39.0
12-13	37.57077274487142	39.0	38.0	39.0	35.0	39.0
14-15	38.97722490331513	41.0	39.0	41.0	36.0	41.0
16-17	38.93011386288956	41.0	39.0	41.0	36.0	41.0
18-19	38.91894361260998	41.0	39.0	41.0	35.5	41.0
20-21	38.89051948594022	41.0	39.0	41.0	35.0	41.0
22-23	38.80260369064874	41.0	39.0	41.0	35.0	41.0
24-25	38.72532589312058	41.0	39.0	41.0	35.0	41.0
26-27	38.60051051911172	40.5	38.0	41.0	35.0	41.0
28-29	38.461242790914575	40.0	38.0	41.0	34.0	41.0
30-31	38.246159736205406	40.0	38.0	41.0	34.0	41.0
32-33	38.004716148783146	40.0	38.0	41.0	34.0	41.0
34-35	37.85310960400585	40.0	38.0	41.0	33.0	41.0
36-37	37.70297819606985	40.0	37.5	41.0	33.0	41.0
38-39	37.51133699298918	40.0	37.0	41.0	33.0	41.0
40-41	37.27453154438148	40.0	37.0	41.0	32.5	41.0
42-43	37.014819663117834	40.0	36.5	41.0	31.5	41.0
44-45	36.6699879674661	40.0	36.0	41.0	31.0	41.0
46-47	36.25237304309403	39.5	35.5	41.0	30.0	41.0
48-49	35.87636369916938	39.0	35.0	41.0	28.5	41.0
50-51	34.600864226672215	38.0	34.0	40.0	26.0	40.5
52-53	34.537780429285114	38.0	34.0	39.5	26.0	40.0
54-55	35.36493430322849	39.0	35.0	40.5	28.0	41.0
56-57	35.38024527082557	39.0	35.0	41.0	28.0	41.0
58-59	35.172067249774386	38.5	35.0	41.0	28.0	41.0
60-61	34.837823536269596	38.0	35.0	40.5	27.0	41.0
62-63	34.47015694108808	37.0	35.0	40.0	26.0	41.0
64-65	34.166673191485685	36.5	34.5	40.0	26.0	41.0
66-67	33.85005498119557	36.0	34.0	39.0	26.0	41.0
68-69	33.61520093324103	36.0	34.0	39.0	26.0	41.0
70-71	33.267493519559345	35.0	34.0	38.5	25.5	41.0
72-73	32.89296211910769	35.0	34.0	37.0	25.0	39.5
74-75	32.530940307981055	35.0	34.0	37.0	24.0	39.0
76-77	32.1681163039395	35.0	34.0	36.5	23.0	39.0
78-79	31.827483905046613	35.0	33.5	36.0	21.0	37.5
80-81	31.51500821119688	35.0	33.0	35.5	20.0	37.0
82-83	31.254091781174843	35.0	33.0	35.0	18.5	37.0
84-85	30.995112430787742	35.0	33.0	35.0	16.0	36.0
86-87	30.748096096191183	35.0	33.0	35.0	8.5	36.0
88-89	30.5147097007267	35.0	33.0	35.0	2.0	36.0
90-91	30.272887505883133	35.0	32.5	35.0	2.0	35.5
92-93	29.992478370944596	35.0	32.0	35.0	2.0	35.0
94-95	29.67551422530488	35.0	32.0	35.0	2.0	35.0
96-97	29.373983459583783	35.0	31.5	35.0	2.0	35.0
98-99	29.056016986598692	35.0	31.0	35.0	2.0	35.0
100	28.48307255646727	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4831415671229493
1101	2	0.5383087363326524
1101	3	0.5513758278855008
1101	4	0.47906215211808956
1101	5	0.5358439559579153
1101	6	0.4977617733223667
1101	7	0.47611838467552303
1101	8	0.37744220885701907
1101	9	0.49481820644647456
1101	10-11	0.44402235107078525
1101	12-13	0.528879929416199
1101	14-15	0.6794422850690651
1101	16-17	0.6766007671631016
1101	18-19	0.6075280030059886
1101	20-21	0.6702170745831282
1101	22-23	0.6902398344272669
1101	24-25	0.5808467966124908
1101	26-27	0.7254532034773078
1101	28-29	0.6647571754547883
1101	30-31	0.6733850266399131
1101	32-33	0.6731856561338319
1101	34-35	0.7541002187966299
1101	36-37	0.7871077133030582
1101	38-39	0.8056747984094059
1101	40-41	0.784157000710799
1101	42-43	0.7561839145035023
1101	44-45	0.8053916088425481
1101	46-47	0.8751796937794367
1101	48-49	0.7802340261678253
1101	50-51	0.7380990383881851
1101	52-53	0.7686107328391927
1101	54-55	0.8714798832031576
1101	56-57	0.9422834355213396
1101	58-59	0.9234072015845953
1101	60-61	0.8609524803116315
1101	62-63	0.8887069061199924
1101	64-65	0.871786789264732
1101	66-67	0.8315739364716634
1101	68-69	0.8189454397466989
1101	70-71	0.788280160981941
1101	72-73	0.7516256996703916
1101	74-75	0.651680690928444
1101	76-77	0.6324592287140547
1101	78-79	0.7134214367659375
1101	80-81	0.6626385915734012
1101	82-83	0.5748267815365189
1101	84-85	0.5354276263320408
1101	86-87	0.47919593581275777
1101	88-89	0.45322500414972566
1101	90-91	0.5476126890854651
1101	92-93	0.5568133678855318
1101	94-95	0.4942012430349507
1101	96-97	0.4119048218849741
1101	98-99	0.359092371720525
1101	100	0.38395455209284535
1102	1	0.496771356064734
1102	2	0.5783081182046388
1102	3	0.5993672335185423
1102	4	0.47801458431584365
1102	5	0.46436776479638553
1102	6	0.46353834007624073
1102	7	0.5000627536852136
1102	8	0.4877677454428664
1102	9	0.45949268809937394
1102	10-11	0.5358929364214333
1102	12-13	0.5395688019115781
1102	14-15	0.685220952927601
1102	16-17	0.7031640390977074
1102	18-19	0.699237656208993
1102	20-21	0.6475566808103821
1102	22-23	0.6735434805932883
1102	24-25	0.6886938521112924
1102	26-27	0.6869689150378235
1102	28-29	0.6540322399652609
1102	30-31	0.687457437020143
1102	32-33	0.7800600885354712
1102	34-35	0.7849749833425861
1102	36-37	0.7736608093384589
1102	38-39	0.8151679712452236
1102	40-41	0.8689089168247293
1102	42-43	0.8721084837853468
1102	44-45	0.9088748596626459
1102	46-47	0.8900793975356152
1102	48-49	0.9140405347241582
1102	50-51	0.9491058057624073
1102	52-53	0.9501030981292331
1102	54-55	0.9812896609403694
1102	56-57	0.9587734095447118
1102	58-59	1.0276597299569659
1102	60-61	0.9817935714720534
1102	62-63	0.9104067143742967
1102	64-65	0.8387536337935302
1102	66-67	0.8013676886433174
1102	68-69	0.8218277103033245
1102	70-71	0.7771667731582781
1102	72-73	0.7301849978112429
1102	74-75	0.6801078973685364
1102	76-77	0.6242000403439789
1102	78-79	0.6764899462411087
1102	80-81	0.692045849258438
1102	82-83	0.6370622905632786
1102	84-85	0.6444431560024206
1102	86-87	0.7141554288628953
1102	88-89	0.601600033820862
1102	90-91	0.6777556285348503
1102	92-93	0.6500751226143251
1102	94-95	0.6865643709563436
1102	96-97	0.7075434114574577
1102	98-99	0.761214620064333
1102	100	0.7485236004734972
1103	1	0.46135623363485934
1103	2	0.5600207900733878
1103	3	0.5882017644385797
1103	4	0.42670380517252227
1103	5	0.49589858646512397
1103	6	0.48622138454857833
1103	7	0.5150487060160032
1103	8	0.4576546042824745
1103	9	0.5659521457824894
1103	10-11	0.5587801437715569
1103	12-13	0.5688861158929157
1103	14-15	0.723546166334792
1103	16-17	0.7422555160112481
1103	18-19	0.7290532064069168
1103	20-21	0.7178730712044512
1103	22-23	0.7190125968267651
1103	24-25	0.6891112455190154
1103	26-27	0.7142024356588266
1103	28-29	0.7200248455138265
1103	30-31	0.7657624133038539
1103	32-33	0.7953328913511157
1103	34-35	0.7304562252157041
1103	36-37	0.7239734526034596
1103	38-39	0.8069716579524666
1103	40-41	0.7987657185862815
1103	42-43	0.811205567009317
1103	44-45	0.850732573942679
1103	46-47	0.8334632289363242
1103	48-49	0.8711166459070796
1103	50-51	0.7970434839769496
1103	52-53	0.8702349367883144
1103	54-55	0.9653517927428865
1103	56-57	0.9324290503841439
1103	58-59	0.9378403832268987
1103	60-61	0.8825052352054854
1103	62-63	0.876024710199296
1103	64-65	0.8357707045554292
1103	66-67	0.8231651830821889
1103	68-69	0.8033842153150133
1103	70-71	0.7470044321681613
1103	72-73	0.7658669124541149
1103	74-75	0.8101453298872272
1103	76-77	0.807086282145498
1103	78-79	0.7814429229605686
1103	80-81	0.7904413344681878
1103	82-83	0.7818436785381557
1103	84-85	0.7768803426564084
1103	86-87	0.7415012632991989
1103	88-89	0.7490334286328775
1103	90-91	0.805266672466125
1103	92-93	0.9093853031675394
1103	94-95	0.8350449857856823
1103	96-97	0.897800732038796
1103	98-99	0.8355483242959032
1103	100	0.9208525156089671
1104	1	0.3648892540135975
1104	2	0.4850067765271362
1104	3	0.4924984748864034
1104	4	0.4200293607977983
1104	5	0.422858359144783
1104	6	0.3681554607605335
1104	7	0.3666897136077978
1104	8	0.37917529811573303
1104	9	0.4556476875408677
1104	10-11	0.5096258117716346
1104	12-13	0.5001753790917931
1104	14-15	0.601570428086454
1104	16-17	0.5950113410833993
1104	18-19	0.629865066032842
1104	20-21	0.5742825844772028
1104	22-23	0.5982639713929387
1104	24-25	0.5984940961868972
1104	26-27	0.6199867379396338
1104	28-29	0.6948113003361058
1104	30-31	0.6695365882048279
1104	32-33	0.6935878265324078
1104	34-35	0.6522181758172039
1104	36-37	0.6014142693983544
1104	38-39	0.6784360931086866
1104	40-41	0.631789275716848
1104	42-43	0.6208448185404691
1104	44-45	0.5660986508894013
1104	46-47	0.4392627278958088
1104	48-49	0.4684550390741933
1104	50-51	0.4225456141411499
1104	52-53	0.44347464607778164
1104	54-55	0.5252468784022568
1104	56-57	0.5190728320563025
1104	58-59	0.49370284235916984
1104	60-61	0.5647907689372644
1104	62-63	0.5111810256550058
1104	64-65	0.4373425062037697
1104	66-67	0.47731147504000404
1104	68-69	0.4344233321852471
1104	70-71	0.38267630122876284
1104	72-73	0.42231070151135697
1104	74-75	0.3991693256824078
1104	76-77	0.40265894511425415
1104	78-79	0.38125349323506796
1104	80-81	0.3595718495742446
1104	82-83	0.3431177853725025
1104	84-85	0.2700316418643496
1104	86-87	0.2871398223619295
1104	88-89	0.2401912081346218
1104	90-91	0.26646501208437456
1104	92-93	0.18582920381900436
1104	94-95	0.06937920240770268
1104	96-97	0.064561085265467
1104	98-99	0.010628902917186878
1104	100	0.08679951641793693
1105	1	0.3373496787919166
1105	2	0.360281764446448
1105	3	0.39752696146931754
1105	4	0.3828698507250934
1105	5	0.3643806011754407
1105	6	0.3678111572098359
1105	7	0.3953001893958259
1105	8	0.37803341919416766
1105	9	0.3905990045830947
1105	10-11	0.4405100264940387
1105	12-13	0.43727191582692626
1105	14-15	0.5316618959880017
1105	16-17	0.5271004719135348
1105	18-19	0.5153163628031336
1105	20-21	0.4869011393472533
1105	22-23	0.4921405292853649
1105	24-25	0.48956670310695927
1105	26-27	0.4105756624855985
1105	28-29	0.460752804806944
1105	30-31	0.49198550959454934
1105	32-33	0.5236803825204461
1105	34-35	0.5218524196705303
1105	36-37	0.6174536085398543
1105	38-39	0.5768811244163103
1105	40-41	0.6155013394088087
1105	42-43	0.5249859426652179
1105	44-45	0.6290836541313141
1105	46-47	0.5627404842689359
1105	48-49	0.6007708111351349
1105	50-51	0.5575349530219356
1105	52-53	0.5686604986975539
1105	54-55	0.5968563104785787
1105	56-57	0.594030617493317
1105	58-59	0.5577397113049258
1105	60-61	0.5757138802239865
1105	62-63	0.638465062835742
1105	64-65	0.6486963347943373
1105	66-67	0.655847072387779
1105	68-69	0.5727053564325999
1105	70-71	0.539117704020704
1105	72-73	0.504246460034274
1105	74-75	0.5269069805298798
1105	76-77	0.5151417738326245
1105	78-79	0.5142284621381386
1105	80-81	0.5062964538752333
1105	82-83	0.41810248033211295
1105	84-85	0.38934942281492724
1105	86-87	0.3483818254583717
1105	88-89	0.3366813736950931
1105	90-91	0.4036217979503398
1105	92-93	0.43321961861246905
1105	94-95	0.4049804289817871
1105	96-97	0.3960737138259418
1105	98-99	0.2990413618638712
1105	100	0.33634826646994753
1106	1	0.2679602334704825
1106	2	0.2994074215063094
1106	3	0.3627680519994456
1106	4	0.3383379326367262
1106	5	0.3083429095363428
1106	6	0.33447495630786506
1106	7	0.3237927612733529
1106	8	0.32496871794464965
1106	9	0.34899947657503105
1106	10-11	0.38484828789808034
1106	12-13	0.4387867390750344
1106	14-15	0.515357803746042
1106	16-17	0.547850649697537
1106	18-19	0.5332237670795976
1106	20-21	0.5052544436752129
1106	22-23	0.5246375722416303
1106	24-25	0.5238171385719568
1106	26-27	0.5296652342622039
1106	28-29	0.5326242896453905
1106	30-31	0.5216957439259957
1106	32-33	0.5431649050834793
1106	34-35	0.5292865812606848
1106	36-37	0.5304470729814668
1106	38-39	0.49625579816166265
1106	40-41	0.48618843270387657
1106	42-43	0.5131659168997871
1106	44-45	0.5401196088581415
1106	46-47	0.548352331293664
1106	48-49	0.515705826508075
1106	50-51	0.5784077199715725
1106	52-53	0.6178959165350051
1106	54-55	0.6442818252214977
1106	56-57	0.7020845692409878
1106	58-59	0.6559840316939471
1106	60-61	0.6588582446645859
1106	62-63	0.6509935082152154
1106	64-65	0.5748208342118062
1106	66-67	0.595516298224041
1106	68-69	0.6086001286771108
1106	70-71	0.5771815732549257
1106	72-73	0.5207243162642143
1106	74-75	0.55671855798699
1106	76-77	0.5335671716010566
1106	78-79	0.5465243346849924
1106	80-81	0.593646200291861
1106	82-83	0.5962339726071555
1106	84-85	0.5550020438037926
1106	86-87	0.5505065968057501
1106	88-89	0.5712085388308843
1106	90-91	0.5759579962571912
1106	92-93	0.6185645416444459
1106	94-95	0.5136308463245953
1106	96-97	0.6512968017326699
1106	98-99	0.7125535959084992
1106	100	0.8032003088777238
1107	1	0.2729480804631734
1107	2	0.2995907950852157
1107	3	0.32853626626308596
1107	4	0.31991102911823077
1107	5	0.35509127180698385
1107	6	0.31428290178406826
1107	7	0.30746498487769003
1107	8	0.3073661841437101
1107	9	0.31069968682807314
1107	10-11	0.3144263537675158
1107	12-13	0.32044094500815845
1107	14-15	0.3318002314517514
1107	16-17	0.38794877619924506
1107	18-19	0.41068275020098355
1107	20-21	0.42425868772777875
1107	22-23	0.4004479849669593
1107	24-25	0.3934666858785718
1107	26-27	0.4350248590153072
1107	28-29	0.463419515675632
1107	30-31	0.4197997837297862
1107	32-33	0.3352844255300127
1107	34-35	0.40824742013801085
1107	36-37	0.4445091669355392
1107	38-39	0.491679065447002
1107	40-41	0.45813526148817374
1107	42-43	0.47389355525138654
1107	44-45	0.4296364190269486
1107	46-47	0.3454130777584581
1107	48-49	0.3884074304994911
1107	50-51	0.4068285939792702
1107	52-53	0.47433465513213946
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2316	74-75	-0.8868191675233916
2316	76-77	-0.8677254787882731
2316	78-79	-0.8212828121398736
2316	80-81	-0.7844217668518034
2316	82-83	-0.7039797687202203
2316	84-85	-0.8329375609703327
2316	86-87	-0.8032559100786223
2316	88-89	-0.7671826736982901
2316	90-91	-0.7087829569345523
2316	92-93	-0.7083196102534899
2316	94-95	-0.5892503056499052
2316	96-97	-0.8499984418744901
2316	98-99	-0.6283086088442573
2316	100	-0.6690876539964421
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19630.0
3	2914.0
4	2922.0
5	3314.0
6	4044.0
7	4722.0
8	6033.0
9	7013.0
10	9142.0
11	13020.0
12	19415.0
13	28041.0
14	29826.0
15	36376.0
16	47461.0
17	66095.0
18	67159.0
19	39161.0
20	32741.0
21	30040.0
22	30998.0
23	35315.0
24	39614.0
25	43956.0
26	50075.0
27	58731.0
28	70423.0
29	85103.0
30	103367.0
31	128403.0
32	163379.0
33	221353.0
34	322514.0
35	523929.0
36	963042.0
37	1721962.0
38	1575598.0
39	340535.0
40	464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.69784691056112	17.95837819476541	13.356149879295497	23.987625015377976
2	15.373372508785899	21.60936804467803	36.92411016623779	26.093149280298285
3	18.908477677494638	26.71252422656506	29.496498831019068	24.882499264921233
4	13.539399197515314	17.98388534530733	34.687028807309126	33.78968664986824
5	14.034005247104728	36.36755609072359	33.26265572694378	16.3357829352279
6	31.42891621712336	34.16037479051109	17.97528751740214	16.43542147496341
7	25.98097362372985	32.12018987153764	22.023264137334145	19.875572367398366
8	27.469314436943502	31.062307834575957	21.127547315814184	20.340830412666353
9	27.761119033048924	14.534697486698525	19.817053955439494	37.88712952481305
10-11	25.051810965726972	25.399991907297736	26.36977460814407	23.17842251883122
12-13	27.074031359307547	23.49680712931496	26.54684825981171	22.882313251565776
14-15	25.41894793905137	23.308057015529833	25.116648560662803	26.156346484755993
16-17	23.148381341505132	26.357954595419347	26.907394451627653	23.586269611447864
18-19	24.231143475307263	25.26196161046833	28.633760989908385	21.873133924316026
20-21	24.58564018008044	24.745036523490967	27.86808129869763	22.801241997730965
22-23	23.5270353795827	25.372118629857752	26.392527970082586	24.70831802047696
24-25	23.302745612118564	26.173217933547775	26.049720549944443	24.474315904389215
26-27	24.00428161303895	24.961465122122952	26.37449116187244	24.659762102965654
28-29	23.450284894027817	24.415900346125383	28.324522783840813	23.809291976005994
30-31	24.670560462262625	24.59476586540366	27.471894129703063	23.262779542630653
32-33	22.46009118902114	25.382819554493313	27.631032195294214	24.526057061191334
34-35	22.48043113840819	25.796250603091835	27.964483782937965	23.758834475562004
36-37	23.7865528201756	25.62429648942729	26.805869928615795	23.78328076178132
38-39	24.256660138838765	24.56280112190933	26.772468825184788	24.408069914067116
40-41	23.41936890311024	25.495740606049427	26.84269001432765	24.242200476512675
42-43	24.855284707496345	24.959192658064953	26.57638398026318	23.60913865417552
44-45	24.523505917239717	24.706328717123693	26.810520418035104	23.959644947601486
46-47	23.50399057713396	25.01816652019756	27.943508089356566	23.53433481331192
48-49	24.183361921765723	25.63670619144245	27.04347196499524	23.136459921796586
50-51	23.04714761314456	26.108782368265487	26.2884653343852	24.555604684204756
52-53	22.885403050485753	26.220496262815512	25.208825281490554	25.685275405208174
54-55	23.507847893683778	25.90362040218272	25.14979160227802	25.43874010185548
56-57	23.95138229286863	24.773095702231352	25.397227531463106	25.87829447343691
58-59	23.706421333365622	24.57563441694352	26.838785400673725	24.879158849017134
60-61	23.857093522513438	25.07904219755646	26.248314087080903	24.8155501928492
62-63	22.273053441171427	25.382136780612434	26.70120691234433	25.643602865871813
64-65	22.283077091560415	25.411244089291596	26.757239289396495	25.548439529751498
66-67	23.762153305655907	25.221861323703394	25.835265517395424	25.180719853245275
68-69	24.1099961436282	24.568733649772426	25.766723149462035	25.554547057137338
70-71	23.142932867690806	25.141468318936695	26.06388038259859	25.65171843077391
72-73	24.26521959531659	24.864264983180295	25.947651227588597	24.922864193914517
74-75	24.552852539598565	24.48397104655525	26.07583078702063	24.887345626825553
76-77	23.708552066565407	25.29967344920815	26.7618455854582	24.229928898768247
78-79	24.030358211230425	25.252181678250867	26.51805658240889	24.199403528109816
80-81	23.313962753043903	25.280365052328374	26.332241880161845	25.073430314465877
82-83	23.40765608494932	25.780930134778107	25.162637698909435	25.648776081363135
84-85	23.67922692692799	25.75934363417848	24.975580856256403	25.585848582637126
86-87	23.969942372074073	24.706364494558596	25.465556017881262	25.85813711548607
88-89	24.120388889541683	24.308371727790583	26.196792952370064	25.37444643029767
90-91	24.66313593763348	24.86289739174692	25.54270630993159	24.93126036068801
92-93	23.830539392107024	25.056217000793463	25.78970403139736	25.323539575702153
94-95	23.477639598621664	25.26200235154067	26.114690441028355	25.145667608809312
96-97	24.843952922502957	24.912582506996397	25.15480839366831	25.088656176832338
98-99	25.03698261195803	24.48980181713392	25.06646464728213	25.40675092362592
100	24.707012021376347	24.959007849732938	25.392638443018235	24.941341685872477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2846.0
1	3518.5
2	2754.0
3	1158.0
4	894.5
5	939.0
6	1291.5
7	2120.0
8	3264.0
9	3557.0
10	2850.0
11	2459.0
12	2727.0
13	3107.0
14	3429.5
15	3711.0
16	4179.0
17	4809.0
18	5508.5
19	6354.5
20	7512.0
21	8978.5
22	10846.5
23	13303.0
24	16241.0
25	19767.5
26	24637.5
27	30134.0
28	35560.0
29	42229.5
30	49526.0
31	56132.0
32	62951.5
33	69977.0
34	76870.5
35	82718.0
36	89307.0
37	97665.5
38	104419.5
39	111947.0
40	119738.0
41	127389.0
42	136348.0
43	152853.5
44	173857.5
45	191988.0
46	218071.0
47	251310.5
48	328392.5
49	427324.0
50	538961.0
51	505851.0
52	353105.5
53	296050.0
54	268668.5
55	242012.5
56	222955.5
57	200495.0
58	185762.0
59	170614.0
60	146227.5
61	121461.5
62	99916.5
63	79200.0
64	59313.5
65	47522.0
66	38050.5
67	28835.0
68	23202.0
69	19922.0
70	17254.5
71	15103.5
72	13193.5
73	12808.5
74	10527.5
75	8396.5
76	6656.0
77	4649.5
78	3421.0
79	2483.5
80	1798.0
81	1351.5
82	1050.0
83	815.0
84	588.5
85	388.5
86	266.5
87	194.0
88	143.0
89	103.0
90	85.5
91	74.0
92	67.0
93	60.5
94	51.5
95	39.0
96	24.5
97	21.5
98	20.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0875669093803389
2	0.28328269402101086
3	0.2878740556403942
4	0.2964234876213149
5	0.32365501170869176
6	0.1713052852473362
7	0.18762692812000292
8	0.15505560729033382
9	0.20439475346978841
10-11	0.22537252638593636
12-13	0.24239942543211332
14-15	0.17019702554610577
16-17	0.16017950928563307
18-19	0.17731435570530657
20-21	0.20846796769638867
22-23	0.17180903965698643
24-25	0.19682404434190243
26-27	0.19470107932980513
28-29	0.2233143297979369
30-31	0.2578718822999411
32-33	0.22662471591849542
34-35	0.15344359317945316
36-37	0.14826211925162244
38-39	0.1159138896605127
40-41	0.10659443308198387
42-43	0.122556251376329
44-45	0.13554591865373794
46-47	0.20234375337335542
48-49	0.20687034656864087
50-51	0.21280745211094687
52-53	0.1418140628080998
54-55	0.08542955138510874
56-57	0.12480155674505565
58-59	0.14397301027802925
60-61	0.1200446758196444
62-63	0.14157657858640754
64-65	0.11651119846052653
66-67	0.12032533899073523
68-69	0.1060546962145015
70-71	0.13204122726088577
72-73	0.15654528104458515
74-75	0.19367917752737185
76-77	0.174953906471517
78-79	0.19992573220703444
80-81	0.10414762594939714
82-83	0.14527557525155335
84-85	0.1753856959655029
86-87	0.18832498780194679
88-89	0.17018982905453933
90-91	0.17354339412449643
92-93	0.1244705181329998
94-95	0.36176043455294676
96-97	0.36212025913126833
98-99	0.4082137876142623
100	0.4411737189885187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6947830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.99992102389842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10937068292763	34.016966916161934
2	11.874915608345589	10.21240866648638
3	3.5800283728558027	4.618228118883453
4	1.5839618832632623	2.7244094354074275
5	0.8823953762791893	1.8971465745929137
6	0.5737069007392964	1.4801610853593168
7	0.38061870786709845	1.145660206495245
8	0.27648786304362916	0.9511165019953999
9	0.2187087755629998	0.8464014068798265
>10	1.2828341741714593	10.284006354933567
>50	0.11601307713126831	3.479487100546318
>100	0.0990467268455296	8.743913538122335
>500	0.012636308023211099	3.6973894274620625
>1k	0.007931261358078583	6.136776327096554
>5k	6.385337533691639E-4	2.013761371846597
>10k+	7.057478326711812E-4	7.7521669677307505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	79227	1.140312874667342	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	73496	1.057826688332904	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	34351	0.49441336359697924	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	32533	0.46824692026143416	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28101	0.40445721901658505	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	26318	0.3787945300906902	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	25794	0.3712526069290699	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	24419	0.35146225512138324	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	23841	0.34314311087058835	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23625	0.3400342265138899	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	23567	0.33919943349218384	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	22823	0.3284910540413338	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	19905	0.28649232925963936	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	15963	0.22975518974989312	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15945	0.22949611605350162	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	12485	0.1796963944137954	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11559	0.1663684920327642	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	11338	0.16318764276040146	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	10949	0.15758877232171772	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	10176	0.14646299636001456	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	10030	0.14436162082261655	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	9856	0.1418572417574984	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	9757	0.14043233642734493	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	9385	0.1350781467019199	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9250	0.13313509397898338	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	8315	0.1196776547497564	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	7661	0.11026464378086395	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7563	0.10885413143384337	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	7401	0.10652246816631955	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	7389	0.10634975236872521	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	7374	0.10613385762173226	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	7105	0.10226214515899208	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.757193253145227E-5	0.0	0.0	1.4392983132863067E-5	0.0
2	8.63578987971784E-5	0.0	0.0	1.4392983132863067E-5	0.0
3	1.1514386506290453E-4	0.0	0.0	1.4392983132863067E-5	0.0
4	1.727157975943568E-4	0.0	0.0	5.757193253145227E-5	1.4392983132863067E-5
5	0.0010362947855661409	1.4392983132863067E-5	0.0	7.196491566431533E-5	1.4392983132863067E-5
6	0.002547558014516763	1.4392983132863067E-5	0.0	1.0075088193004147E-4	1.4392983132863067E-5
7	0.002835417677174024	1.4392983132863067E-5	0.0	1.0075088193004147E-4	1.4392983132863067E-5
8	0.0031088843566984222	1.4392983132863067E-5	0.0	1.1514386506290453E-4	2.8785966265726133E-5
9	0.003396744019355684	1.4392983132863067E-5	0.0	1.1514386506290453E-4	4.31789493985892E-5
10-11	0.005764389744711659	1.4392983132863067E-5	0.0	1.583228144614937E-4	5.757193253145227E-5
12-13	0.011154561927968877	1.4392983132863067E-5	0.0	2.0869825542651447E-4	7.196491566431533E-5
14-15	0.01773935171125373	4.31789493985892E-5	0.0	3.4543159518871363E-4	1.0794737349647301E-4
16-17	0.020344481658301945	4.31789493985892E-5	0.0	4.965579180837758E-4	1.295368481957676E-4
18-19	0.021884530853518293	4.31789493985892E-5	0.0	6.62077224111701E-4	1.4392983132863066E-4
20-21	0.027764064463292853	4.31789493985892E-5	0.0	0.0010650807518318669	1.4392983132863066E-4
22-23	0.03003095930671879	4.31789493985892E-5	0.0	0.003202438747062032	1.6551930602792526E-4
24-25	0.044236833658854634	5.757193253145227E-5	0.0	0.008700558303815723	1.8710878072721986E-4
26-27	0.08845207784300997	5.757193253145227E-5	0.0	0.01769617276185514	1.8710878072721986E-4
28-29	0.09829687830588832	5.757193253145227E-5	0.0	0.053556290237383467	1.8710878072721986E-4
30-31	0.10018235909629337	7.196491566431533E-5	0.0	0.1515868983553138	1.8710878072721986E-4
32-33	0.10127622581439097	7.196491566431533E-5	0.0	0.30080615098527164	2.0150176386008294E-4
34-35	0.10223335919272636	1.07947373496473E-4	0.0	0.4983138620259851	2.0150176386008294E-4
36-37	0.10390294523613847	1.4392983132863066E-4	0.0	0.7845039386398343	2.0150176386008294E-4
38-39	0.10572365760244565	1.4392983132863066E-4	0.0	1.1769430167404786	2.0150176386008294E-4
40-41	0.10748679803622138	1.4392983132863066E-4	0.0	1.687051928443845	2.0150176386008294E-4
42-43	0.1108907385471435	1.511263228950622E-4	0.0	2.0807935715180137	2.15894746992946E-4
44-45	0.1147480580267508	1.5832281446149374E-4	0.0	2.498751408713224	2.15894746992946E-4
46-47	0.11649680547739366	1.5832281446149374E-4	0.0	2.9772245434905575	2.15894746992946E-4
48-49	0.11793610379067997	1.5832281446149374E-4	0.0	3.4079489567246175	2.15894746992946E-4
50-51	0.11986476353048361	1.5832281446149374E-4	0.0	3.856643009400057	2.15894746992946E-4
52-53	0.12076432497628756	1.5832281446149374E-4	0.0	4.362232524399705	2.446807132586721E-4
54-55	0.12307439876911208	1.5832281446149374E-4	0.0	4.825046093528483	2.7346667952439827E-4
56-57	0.12799679900055125	1.5832281446149374E-4	0.0	5.31432116214703	2.8066317109082977E-4
58-59	0.12973115346806124	1.5832281446149374E-4	0.0	5.816470754177923	2.878596626572613E-4
60-61	0.13060912543916592	1.5832281446149374E-4	0.0	6.277614737263289	2.878596626572613E-4
62-63	0.13112727283194897	1.5832281446149374E-4	0.0	6.742177053842711	2.878596626572613E-4
64-65	0.13178215356449424	1.5832281446149374E-4	0.0	7.245305368726639	2.878596626572613E-4
66-67	0.1325233921958367	1.727157975943568E-4	0.0	7.76637453708568	2.878596626572613E-4
68-69	0.13335818521754272	1.727157975943568E-4	0.0	8.351449301436563	2.878596626572613E-4
70-71	0.13450242737660537	1.727157975943568E-4	0.0	9.026149171755785	3.166456289229875E-4
72-73	0.13696362749232494	1.727157975943568E-4	0.0	9.595758675730409	3.310386120558505E-4
74-75	0.1394176311164781	1.727157975943568E-4	0.0	10.148780266644405	3.310386120558505E-4
76-77	0.14023083466348485	1.727157975943568E-4	0.0	10.747370042157048	3.310386120558505E-4
78-79	0.1406986066153029	1.727157975943568E-4	0.0	11.33518666979474	3.454315951887136E-4
80-81	0.1417492943840019	1.727157975943568E-4	0.0	11.976466033279456	3.598245783215766E-4
82-83	0.1422242628273864	1.727157975943568E-4	0.0	12.685817010491046	3.7421756145443973E-4
84-85	0.14379309798886847	1.727157975943568E-4	0.0	13.348477150419628	3.886105445873028E-4
86-87	0.14717544902509128	1.727157975943568E-4	0.0	14.008330658637302	4.0300352772016583E-4
88	0.1481325824034267	1.8710878072721986E-4	0.0	14.528392318177042	4.31789493985892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	5970	0.0	25.08181	1
TCTACAC	8270	0.0	21.042799	3
AGTACTT	25920	0.0	20.660921	12-13
ATCTACA	7485	0.0	20.61262	2
TACACTC	7990	0.0	20.486954	5
GAGTACT	25265	0.0	19.913048	12-13
CTACACT	9935	0.0	18.79704	4
TACTTTT	29950	0.0	18.001827	14-15
GTACTTT	28245	0.0	17.840887	14-15
GTATAAT	1800	0.0	17.52703	1
GTATAAG	1975	0.0	17.166096	1
CATGGGG	14165	0.0	17.102417	4
GTATATA	1490	0.0	17.065287	1
CACTCTT	10160	0.0	16.100485	7
ACTTTTT	31775	0.0	16.083523	16-17
CATGGGT	6960	0.0	15.612397	4
GTATTAG	2820	0.0	15.528873	1
ATGGGTA	4560	0.0	15.369735	5
GTATAGA	1330	0.0	15.223794	1
ACACTCT	11120	0.0	14.837329	6
>>END_MODULE