##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765263_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8001299 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.081306547849294 33.0 31.0 34.0 30.0 34.0 2 32.09165574239883 34.0 31.0 34.0 30.0 34.0 3 32.11016923627026 34.0 31.0 34.0 30.0 34.0 4 35.59392718607316 37.0 35.0 37.0 33.0 37.0 5 35.62269563979549 37.0 35.0 37.0 33.0 37.0 6 35.735840267936496 37.0 36.0 37.0 35.0 37.0 7 35.68653427399726 37.0 35.0 37.0 35.0 37.0 8 35.72899113006525 37.0 36.0 37.0 35.0 37.0 9 37.39674245394404 39.0 38.0 39.0 35.0 39.0 10-11 37.320105335396164 39.0 37.5 39.0 34.5 39.0 12-13 37.306314262221676 39.0 37.5 39.0 34.0 39.0 14-15 38.64748555203349 40.0 38.0 41.0 34.0 41.0 16-17 38.5915646946827 40.0 38.0 41.0 34.0 41.0 18-19 38.57006499819592 40.0 38.0 41.0 34.0 41.0 20-21 38.56630435882973 40.0 38.0 41.0 34.0 41.0 22-23 38.48198373789056 40.0 38.0 41.0 34.0 41.0 24-25 38.4123441706153 40.0 38.0 41.0 34.0 41.0 26-27 38.27829918616965 40.0 38.0 41.0 34.0 41.0 28-29 38.14386226536466 40.0 38.0 41.0 33.5 41.0 30-31 37.92717051318792 40.0 38.0 41.0 33.0 41.0 32-33 37.64216929026149 40.0 37.0 41.0 33.0 41.0 34-35 37.5133951999544 40.0 37.0 41.0 33.0 41.0 36-37 37.38607356130548 40.0 37.0 41.0 32.0 41.0 38-39 37.202440003804384 40.0 37.0 41.0 31.5 41.0 40-41 36.94736554651938 40.0 36.0 41.0 31.0 41.0 42-43 36.69424752155869 40.0 36.0 41.0 30.5 41.0 44-45 36.347021839828756 39.0 35.0 41.0 30.0 41.0 46-47 35.93132052682945 39.0 35.0 41.0 29.0 41.0 48-49 35.54254490427117 39.0 35.0 40.5 27.5 41.0 50-51 34.30951874189428 38.0 34.0 39.5 25.5 40.5 52-53 34.27420472600761 38.0 33.5 39.5 25.5 40.0 54-55 35.100074875342116 39.0 35.0 40.0 26.5 41.0 56-57 35.11591392847586 38.5 35.0 41.0 27.0 41.0 58-59 34.92072024804972 38.0 35.0 41.0 26.0 41.0 60-61 34.56832203121019 37.5 35.0 40.0 26.0 41.0 62-63 34.10745061520635 37.0 34.0 40.0 25.0 41.0 64-65 33.84304692275592 36.5 34.0 39.5 24.5 41.0 66-67 33.556708291991086 36.0 34.0 39.0 24.0 41.0 68-69 33.33556295046592 35.5 34.0 39.0 23.5 41.0 70-71 32.949105139053046 35.0 34.0 38.5 23.0 40.5 72-73 32.56080862619932 35.0 33.5 37.0 22.5 39.5 74-75 32.18445842356347 35.0 33.0 37.0 20.5 39.0 76-77 31.804966043638665 35.0 33.0 36.0 20.0 39.0 78-79 31.50596034468903 35.0 33.0 36.0 19.5 37.0 80-81 31.22432463778694 35.0 33.0 35.5 18.0 37.0 82-83 30.988748202010697 35.0 33.0 35.0 16.0 36.5 84-85 30.75165532496661 35.0 32.0 35.0 11.0 36.0 86-87 30.488677838435983 35.0 32.0 35.0 6.5 36.0 88-89 30.288186330744544 35.0 32.0 35.0 2.0 36.0 90-91 30.049249003193104 35.0 32.0 35.0 2.0 35.0 92-93 29.746161779481056 35.0 31.0 35.0 2.0 35.0 94-95 29.482470158907947 35.0 31.0 35.0 2.0 35.0 96-97 29.23085451499813 35.0 31.0 35.0 2.0 35.0 98-99 28.91173570691459 35.0 31.0 35.0 2.0 35.0 100 28.346563976674286 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48613457868666643 1101 2 0.5893990137191523 1101 3 0.6224336000469961 1101 4 0.44046692837919466 1101 5 0.46858763754633515 1101 6 0.5103840973509151 1101 7 0.5656080110385915 1101 8 0.4866179318710877 1101 9 0.6297320144032099 1101 10-11 0.6001480558250947 1101 12-13 0.6176077577329195 1101 14-15 0.7909456929632839 1101 16-17 0.778575725617145 1101 18-19 0.7500284104924262 1101 20-21 0.7317920930792567 1101 22-23 0.6824317282726611 1101 24-25 0.7738268118046463 1101 26-27 0.7555515947219078 1101 28-29 0.8060354389453792 1101 30-31 0.8490985299668665 1101 32-33 0.8088546185182892 1101 34-35 0.9512691877225521 1101 36-37 1.012628250458441 1101 38-39 0.936148078823102 1101 40-41 1.0340191264702057 1101 42-43 0.9593804343438777 1101 44-45 1.0750472919364 1101 46-47 1.0821155988725835 1101 48-49 1.1804092576810703 1101 50-51 1.0838691600487138 1101 52-53 1.0989128036646392 1101 54-55 1.191785552291826 1101 56-57 1.3196573024030158 1101 58-59 1.3169822758313998 1101 60-61 1.2915415136263135 1101 62-63 1.2845001656328279 1101 64-65 1.2460906493899628 1101 66-67 1.2324500188978007 1101 68-69 1.2387890098906738 1101 70-71 1.1424515467251908 1101 72-73 1.1206235941310112 1101 74-75 1.1365370937198165 1101 76-77 1.0737822814522069 1101 78-79 1.036250824794891 1101 80-81 1.0458329873281471 1101 82-83 1.0439781058693747 1101 84-85 1.0488164607556882 1101 86-87 1.12812348099866 1101 88-89 1.1147526829787928 1101 90-91 1.0639496864607025 1101 92-93 1.1176004739177898 1101 94-95 1.1444152491012183 1101 96-97 1.1684346852770737 1101 98-99 1.2104130503663022 1101 100 1.3131858492217887 1102 1 0.4556606359729827 1102 2 0.5987701550906763 1102 3 0.6199960910936824 1102 4 0.5114966157748668 1102 5 0.48355730801714714 1102 6 0.49519189079009607 1102 7 0.526442678187351 1102 8 0.5213799825669128 1102 9 0.6410953661058159 1102 10-11 0.6070741165816429 1102 12-13 0.7093506526342068 1102 14-15 0.8484868825904996 1102 16-17 0.8110310518933446 1102 18-19 0.8310805719952015 1102 20-21 0.7883604869367673 1102 22-23 0.8173815108895397 1102 24-25 0.784024382357039 1102 26-27 0.8458917083862758 1102 28-29 0.8810255299754388 1102 30-31 0.9615981234323172 1102 32-33 0.943169089186533 1102 34-35 0.8863438730365161 1102 36-37 0.8558330917024861 1102 38-39 0.9420358595509484 1102 40-41 1.0218682794956422 1102 42-43 0.955617941208267 1102 44-45 0.9592694661717402 1102 46-47 0.8735252076254341 1102 48-49 0.9729811094244809 1102 50-51 0.9838722623071092 1102 52-53 1.0663848318942897 1102 54-55 1.0345988607878454 1102 56-57 1.0455581680944448 1102 58-59 1.0691871649396703 1102 60-61 1.0649150421517035 1102 62-63 1.0315094937218703 1102 64-65 1.0082249023957175 1102 66-67 1.0229939248975626 1102 68-69 0.9812462266763546 1102 70-71 0.9136962636513246 1102 72-73 0.8866782978722654 1102 74-75 0.8938388433454278 1102 76-77 0.8319286039414173 1102 78-79 0.8859620444491938 1102 80-81 0.8986511640488075 1102 82-83 0.8970836094597452 1102 84-85 0.9578025965761263 1102 86-87 0.9659098713175069 1102 88-89 0.923096942856791 1102 90-91 0.9644750210849793 1102 92-93 1.0204229657779678 1102 94-95 1.012494385901217 1102 96-97 1.0487727669055857 1102 98-99 1.080465517877439 1102 100 1.1452335710135237 1103 1 0.47497117864340765 1103 2 0.5449723537652886 1103 3 0.626153616248807 1103 4 0.4918326339776158 1103 5 0.5162661231560648 1103 6 0.5122860383228414 1103 7 0.5454670133889579 1103 8 0.57912008982467 1103 9 0.6142414825342826 1103 10-11 0.6683971215985096 1103 12-13 0.6630228125619126 1103 14-15 0.7501579497353461 1103 16-17 0.8128765481238247 1103 18-19 0.8097950801984339 1103 20-21 0.819307564220658 1103 22-23 0.8051606104638722 1103 24-25 0.8175797795560484 1103 26-27 0.8623215151098407 1103 28-29 0.8706652976391851 1103 30-31 0.878784927948935 1103 32-33 0.9405201362978843 1103 34-35 0.963964394347208 1103 36-37 0.9683733782923909 1103 38-39 1.0026830969330973 1103 40-41 0.9532331031150463 1103 42-43 0.9122113805845871 1103 44-45 1.0188749196152713 1103 46-47 1.0007256384397607 1103 48-49 1.1035147989271081 1103 50-51 1.0017567652718924 1103 52-53 1.0518188247727451 1103 54-55 1.19623574306312 1103 56-57 1.1380184418535748 1103 58-59 1.1499777611786541 1103 60-61 1.1963928635269596 1103 62-63 1.2499283554382643 1103 64-65 1.21176578164674 1103 66-67 1.1290783234820054 1103 68-69 1.1048736399733343 1103 70-71 1.155135794680099 1103 72-73 1.1284444137097012 1103 74-75 1.0494916351361994 1103 76-77 1.0430591321347968 1103 78-79 1.0562262055581257 1103 80-81 1.0684296978546683 1103 82-83 1.0934301005653744 1103 84-85 1.075091547198845 1103 86-87 1.065389551967641 1103 88-89 0.9951481521114651 1103 90-91 1.106229763779968 1103 92-93 1.134544359464165 1103 94-95 1.0522318235253216 1103 96-97 1.147786849151533 1103 98-99 1.146624242977051 1103 100 1.2184332273600518 1104 1 0.3546969990970439 1104 2 0.438561566146646 1104 3 0.4549644487139375 1104 4 0.4014673389546459 1104 5 0.43877274488170315 1104 6 0.4071475925195074 1104 7 0.4446980423721101 1104 8 0.427136208857533 1104 9 0.524285345234901 1104 10-11 0.5368289059702107 1104 12-13 0.5119420986341936 1104 14-15 0.6168060337666716 1104 16-17 0.596611077304388 1104 18-19 0.5659972563166136 1104 20-21 0.5404652572322775 1104 22-23 0.5370725863814911 1104 24-25 0.5920038101828311 1104 26-27 0.6254363207876139 1104 28-29 0.6025284719816781 1104 30-31 0.606427203651144 1104 32-33 0.6944806749343257 1104 34-35 0.6601507922126544 1104 36-37 0.6326255320981105 1104 38-39 0.6373697830657434 1104 40-41 0.733699743665575 1104 42-43 0.7269575104701076 1104 44-45 0.7200961776478678 1104 46-47 0.6887750909979857 1104 48-49 0.700317027248019 1104 50-51 0.5526359358954949 1104 52-53 0.6679139276870174 1104 54-55 0.807487591540486 1104 56-57 0.6772383567072922 1104 58-59 0.7117352515834128 1104 60-61 0.6340692305360776 1104 62-63 0.6201717141033569 1104 64-65 0.611564615017663 1104 66-67 0.5905030325670069 1104 68-69 0.6347492884637305 1104 70-71 0.5820780641339169 1104 72-73 0.5895110156178092 1104 74-75 0.6131208238180434 1104 76-77 0.5732049120227423 1104 78-79 0.583520289499937 1104 80-81 0.6342021342818995 1104 82-83 0.633180008279993 1104 84-85 0.651915330195024 1104 86-87 0.6622269550901478 1104 88-89 0.5884480448255509 1104 90-91 0.5781085900782656 1104 92-93 0.6294099462995035 1104 94-95 0.6034606482947638 1104 96-97 0.6734976681893095 1104 98-99 0.6499047169849241 1104 100 0.670603274426707 1105 1 0.2983087688577015 1105 2 0.38368808119604836 1105 3 0.39561728301014654 1105 4 0.33209229668240425 1105 5 0.37023949809597667 1105 6 0.38966695503216897 1105 7 0.38551582965671116 1105 8 0.38533906794534545 1105 9 0.4130609999255199 1105 10-11 0.45340804374780674 1105 12-13 0.4600337288027845 1105 14-15 0.5576525783421786 1105 16-17 0.5313776688258116 1105 18-19 0.5934411029619113 1105 20-21 0.5842512737927734 1105 22-23 0.5262938087755842 1105 24-25 0.5675494566647288 1105 26-27 0.5243516210774715 1105 28-29 0.5527698434562964 1105 30-31 0.5683299242076387 1105 32-33 0.5853416954726711 1105 34-35 0.567508279399604 1105 36-37 0.6428913983616198 1105 38-39 0.6447859039845483 1105 40-41 0.7198892858407433 1105 42-43 0.7359160634771982 1105 44-45 0.6774842468269142 1105 46-47 0.6157291391827471 1105 48-49 0.6583460774642873 1105 50-51 0.5960763297195371 1105 52-53 0.6256094225240787 1105 54-55 0.6207215411037978 1105 56-57 0.6800407571382152 1105 58-59 0.6611905417814512 1105 60-61 0.6005133160955793 1105 62-63 0.6500563783899693 1105 64-65 0.6215638316126189 1105 66-67 0.6417551911885297 1105 68-69 0.6124540673805043 1105 70-71 0.6051005867857597 1105 72-73 0.5681695536866727 1105 74-75 0.5500180337769152 1105 76-77 0.5792519717965483 1105 78-79 0.6369292935974897 1105 80-81 0.6495997415390491 1105 82-83 0.6595183779508282 1105 84-85 0.666838466481714 1105 86-87 0.6455487482021169 1105 88-89 0.7076503730469632 1105 90-91 0.7195418612419999 1105 92-93 0.6847900656203656 1105 94-95 0.6846130419864558 1105 96-97 0.6744214049243382 1105 98-99 0.6419157858656952 1105 100 0.7065231257646509 1106 1 0.22868522552202108 1106 2 0.3015513276808548 1106 3 0.33120066385552605 1106 4 0.26587560170936797 1106 5 0.36309386985679026 1106 6 0.3086789798179481 1106 7 0.2788385812589027 1106 8 0.29977354288512714 1106 9 0.26195012196853185 1106 10-11 0.3681634088574057 1106 12-13 0.34274772484951654 1106 14-15 0.40505212913847544 1106 16-17 0.4194526893533208 1106 18-19 0.39328797741971044 1106 20-21 0.43637588182949116 1106 22-23 0.45769300759580744 1106 24-25 0.48560721157844 1106 26-27 0.4605907035719099 1106 28-29 0.45739131055849924 1106 30-31 0.44756569305825167 1106 32-33 0.44897041813803895 1106 34-35 0.4682948732583867 1106 36-37 0.47868913724322937 1106 38-39 0.4518490322933033 1106 40-41 0.4545264341327311 1106 42-43 0.4422753193933957 1106 44-45 0.518108621542126 1106 46-47 0.4771668905483395 1106 48-49 0.447899858291656 1106 50-51 0.43965954538782626 1106 52-53 0.4206644511296673 1106 54-55 0.5115662198760091 1106 56-57 0.4888002470671111 1106 58-59 0.5666746781347811 1106 60-61 0.5616414224483606 1106 62-63 0.5345503968348595 1106 64-65 0.4909173493972432 1106 66-67 0.47388558219566335 1106 68-69 0.4426664900800148 1106 70-71 0.43009662129501436 1106 72-73 0.38666695081829516 1106 74-75 0.4967057811991751 1106 76-77 0.49822668995427577 1106 78-79 0.4514509384422922 1106 80-81 0.48575495058111073 1106 82-83 0.5036217862524701 1106 84-85 0.592058287590671 1106 86-87 0.5875942396030709 1106 88-89 0.5479305963337815 1106 90-91 0.5477299341446482 1106 92-93 0.5962351453900894 1106 94-95 0.5428622906699552 1106 96-97 0.5428100457029856 1106 98-99 0.48746459117631247 1106 100 0.5164291786365602 1107 1 0.14137025604533449 1107 2 0.25267184674034837 1107 3 0.21807939572558155 1107 4 0.22730473640112336 1107 5 0.29430876794877747 1107 6 0.22207268810998215 1107 7 0.25894278508956603 1107 8 0.19951752488145758 1107 9 0.30076102382966496 1107 10-11 0.3018089918385627 1107 12-13 0.34971291858840914 1107 14-15 0.4042968622762473 1107 16-17 0.3703796366937553 1107 18-19 0.4472222590299637 1107 20-21 0.3969231373359108 1107 22-23 0.39199444141657835 1107 24-25 0.428468455241493 1107 26-27 0.418636321915713 1107 28-29 0.4610708880040306 1107 30-31 0.430592103341759 1107 32-33 0.4224846593072442 1107 34-35 0.45284306690877685 1107 36-37 0.5012028527774461 1107 38-39 0.5461537078004284 1107 40-41 0.3953290843562982 1107 42-43 0.46998559931664374 1107 44-45 0.482294125726753 1107 46-47 0.48191045387240905 1107 48-49 0.5828158174983002 1107 50-51 0.4480667892032315 1107 52-53 0.5379045380822802 1107 54-55 0.5572755134756733 1107 56-57 0.5927226709909519 1107 58-59 0.562316800268654 1107 60-61 0.5648157600622952 1107 62-63 0.6520870087878379 1107 64-65 0.6286398127329349 1107 66-67 0.5591447913534253 1107 68-69 0.5822535195173302 1107 70-71 0.5143395851407107 1107 72-73 0.5522990231611544 1107 74-75 0.48078082714957304 1107 76-77 0.47901821545780976 1107 78-79 0.4832069145124649 1107 80-81 0.5471366445863985 1107 82-83 0.5400734028327108 1107 84-85 0.593812163175329 1107 86-87 0.5670469582506428 1107 88-89 0.610532238675539 1107 90-91 0.5309907147399393 1107 92-93 0.5967683105122745 1107 94-95 0.4370505149816637 1107 96-97 0.5520072911012903 1107 98-99 0.6091825079996731 1107 100 0.6309634946352709 1108 1 0.12818344076538324 1108 2 0.14212916419964472 1108 3 0.10191909387265241 1108 4 0.1506301890487478 1108 5 0.24500965811216702 1108 6 0.2516560946670552 1108 7 0.32869768883204387 1108 8 0.23793110581533483 1108 9 0.3101349742633417 1108 10-11 0.3052285093599849 1108 12-13 0.3614064223029345 1108 14-15 0.3707807553012614 1108 16-17 0.38464161537974917 1108 18-19 0.3828947177726789 1108 20-21 0.3598315103599461 1108 22-23 0.3793048762338387 1108 24-25 0.3582299313222066 1108 26-27 0.39996668770467636 1108 28-29 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2316 52-53 -1.2984214508605163 2316 54-55 -1.5483780060726602 2316 56-57 -1.449394551175729 2316 58-59 -1.4533627362210666 2316 60-61 -1.4735180401933334 2316 62-63 -1.4199183705109775 2316 64-65 -1.3467614184566727 2316 66-67 -1.3198927904920694 2316 68-69 -1.3200149802112833 2316 70-71 -1.4170332593258692 2316 72-73 -1.4055494754364233 2316 74-75 -1.2705281188603763 2316 76-77 -1.2534018970314165 2316 78-79 -1.1655486451903272 2316 80-81 -1.0932669972711793 2316 82-83 -1.1064041512755942 2316 84-85 -1.081732843488048 2316 86-87 -1.1581207490087735 2316 88-89 -1.0703001771665477 2316 90-91 -1.05657328796552 2316 92-93 -1.0256478569648912 2316 94-95 -0.8371549993030811 2316 96-97 -0.8754849979840387 2316 98-99 -1.0968562376895505 2316 100 -1.2647300467728435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23121.0 3 4089.0 4 4116.0 5 4627.0 6 5484.0 7 6429.0 8 8110.0 9 9878.0 10 12584.0 11 17705.0 12 25529.0 13 34247.0 14 36041.0 15 43207.0 16 56044.0 17 74587.0 18 72815.0 19 44976.0 20 40327.0 21 39063.0 22 41852.0 23 48889.0 24 55059.0 25 59704.0 26 67387.0 27 77985.0 28 91548.0 29 109715.0 30 133186.0 31 164128.0 32 209628.0 33 281030.0 34 401815.0 35 637661.0 36 1136621.0 37 1956406.0 38 1634413.0 39 330920.0 40 373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38795740395288 18.05308352634451 13.186386671900104 24.372572397802507 2 14.362598108725694 21.959068552923267 36.95910367687569 26.719229661475346 3 19.0859486939642 27.594529788345255 29.19231866001468 24.127202857675865 4 13.198792866693212 18.65218166914682 34.60836036160808 33.540665102551884 5 13.183194691455851 36.77101757333904 34.00409701509096 16.04169072011415 6 30.9251662351663 33.72143127379984 18.69364208293092 16.659760408102937 7 25.048843972780805 32.103129030824675 22.756553678928405 20.09147331746611 8 27.58699008299977 31.03360722952808 21.69970894331997 19.67969374415218 9 27.881214689124022 14.481679673058483 19.791636400797916 37.84546923701957 10-11 24.516440715113184 25.84516108832462 26.284579980587996 23.3538182159742 12-13 27.180122669202483 23.644295887094632 26.410634453893085 22.764946989809797 14-15 25.92879503521985 23.02089922210492 25.006313118274885 26.043992624400346 16-17 22.99903491048471 26.173605605841622 27.907250035526783 22.920109448146885 18-19 24.47807309943204 25.399664336532666 28.975864675150216 21.146397888885076 20-21 23.913067832718554 25.10380815470674 28.244455068914093 22.73866894366061 22-23 22.641884393162716 25.993496355697133 25.88735349571977 25.477265755420387 24-25 22.91324925124642 26.948724934189062 25.302712790371285 24.835313024193233 26-27 24.133841219256716 24.959310245280257 25.858753814997037 25.048094720465986 28-29 23.264078950597284 24.328279145609987 28.528883564796146 23.87875833899658 30-31 24.912368466588607 24.766906164571743 27.35894330752855 22.961782061311105 32-33 21.689431761172322 25.89863261639531 27.768338550671196 24.643597071761164 34-35 21.478821617581165 26.513698281705533 28.39477992289009 23.61270017782321 36-37 23.834527968370292 26.045366379937164 26.777740339993596 23.342365311698952 38-39 24.589732899684495 24.72593300858454 26.345211393801755 24.33912269792921 40-41 23.088872508360968 26.037323754185177 26.586751760910225 24.287051976543626 42-43 25.353229436491993 25.114840410992183 26.173820494939893 23.35810965757593 44-45 25.155154421958102 24.730337896022284 26.5435143265489 23.570993355470712 46-47 23.496342469878538 25.122368244182784 28.622266489674736 22.759022796263945 48-49 24.374541048899566 25.927301206260495 27.54847071621893 22.149687028621013 50-51 22.64927534526216 26.592822182968206 26.459674743134492 24.298227728635137 52-53 22.13516170430473 26.956037093752975 24.710553041847145 26.19824816009515 54-55 23.212366239361693 26.55991889465328 24.42726445719125 25.800450408793786 56-57 24.26403862515607 24.671315018215374 24.95096029764959 26.11368605897897 58-59 23.73308493394749 24.461924015061612 27.255053328086753 24.549937722904147 60-61 24.068047060642446 25.257859915464664 26.231271519337547 24.44282150455534 62-63 21.601125840962393 25.685533138787708 27.05063844900258 25.66270257124732 64-65 21.351660323053544 25.87088179923614 27.25356321932554 25.523894658384783 66-67 23.783916463432483 25.300779083130475 25.891840882585566 25.02346357085148 68-69 24.264273621818113 24.205392369367463 25.78537264345988 25.744961365354545 70-71 22.62592584296462 25.128813070226602 26.336267841587862 25.90899324522092 72-73 24.386592211076806 24.261893888705764 26.322623630172497 25.028890270044933 74-75 24.577523289844933 23.690088661443117 26.605439047198782 25.12694900151317 76-77 22.911384598624394 24.79995316770688 28.147998811661868 24.14066342200686 78-79 23.3958632718344 24.663270682811557 27.73450792291574 24.206358122438296 80-81 22.102310948350407 24.883917807233853 27.435214793572065 25.578556450843674 82-83 22.094544118463254 25.529138295753544 25.61780277653103 26.758514809252166 84-85 22.630092377904383 25.432987198105987 25.366454427336542 26.57046599665308 86-87 23.00578549934423 23.75268480120828 26.22095544619656 27.020574253250935 88-89 23.08524989279977 23.134927209305864 27.457085326627574 26.322737571266796 90-91 23.72647667818033 23.946117933926075 26.65669824446632 25.670707143427272 92-93 22.054059491074312 24.337755252103666 27.21784175509597 26.39034350172605 94-95 21.426262157064897 24.72319959187702 27.709097503193792 26.141440747864298 96-97 23.40520548754347 24.063198034650455 26.443655500495016 26.087940977311057 98-99 23.762411017858717 23.33201728650716 26.217070444636896 26.688501250997227 100 23.069555676973 23.899348423249503 27.05714015079043 25.973955748987066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1925.0 1 2288.0 2 1829.0 3 895.5 4 717.5 5 738.0 6 939.5 7 1427.5 8 2024.5 9 2160.0 10 1918.5 11 1832.0 12 2011.0 13 2266.5 14 2624.5 15 3064.0 16 3527.0 17 4102.5 18 4815.0 19 5559.5 20 6388.0 21 7656.5 22 9453.5 23 11567.5 24 14116.0 25 17346.0 26 22266.5 27 28234.5 28 34270.5 29 43266.5 30 53573.0 31 62332.5 32 72526.5 33 84263.5 34 96918.5 35 106231.0 36 114677.0 37 125688.0 38 130353.0 39 133463.5 40 137078.5 41 139569.5 42 146210.5 43 164098.5 44 187410.0 45 208947.0 46 239890.0 47 284460.5 48 406555.5 49 568366.5 50 789774.0 51 738512.0 52 436280.0 53 322055.0 54 280353.0 55 245167.5 56 224047.5 57 201129.5 58 184836.5 59 169674.0 60 145747.0 61 120451.0 62 97158.0 63 75230.0 64 55197.5 65 44174.5 66 34734.0 67 25140.5 68 19190.5 69 15342.0 70 12910.5 71 11134.5 72 9423.0 73 9641.5 74 8016.5 75 6183.5 76 4837.5 77 3260.0 78 2339.0 79 1644.0 80 1146.0 81 887.0 82 722.0 83 580.5 84 405.0 85 254.0 86 188.0 87 136.5 88 99.0 89 64.5 90 57.0 91 60.0 92 49.0 93 38.0 94 39.5 95 35.5 96 25.0 97 20.0 98 13.5 99 8.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11375652878363876 2 0.3690775710294041 3 0.37926341710264794 4 0.37515158476142435 5 0.3033507434230367 6 0.13700275417779037 7 0.15102547723813345 8 0.12587956030639524 9 0.20630400138777466 10-11 0.20682891615473936 12-13 0.2193518827380404 14-15 0.14975693321796874 16-17 0.13558423450992146 18-19 0.1542874475756999 20-21 0.1550185788582579 22-23 0.1625798510966782 24-25 0.17528403825428845 26-27 0.16767277413330012 28-29 0.1765588312597742 30-31 0.21800835089402357 32-33 0.21474638055645712 34-35 0.1311974468145735 36-37 0.09117894481883504 38-39 0.08513617601341983 40-41 0.08388637894921812 42-43 0.07454414589431041 44-45 0.09661556204811243 46-47 0.1434892009409972 48-49 0.22173274614534466 50-51 0.17857725351845993 52-53 0.11125693465523535 54-55 0.05980903850737237 56-57 0.07488159110164487 58-59 0.10990715382589752 60-61 0.08611726670881815 62-63 0.09535951599858973 64-65 0.104376801816805 66-67 0.10069614946273098 68-69 0.09052905034545017 70-71 0.12582956842382717 72-73 0.15019436219043933 74-75 0.1926562174466921 76-77 0.1919250861641341 78-79 0.154381182355515 80-81 0.0910664630830569 82-83 0.13123494072649955 84-85 0.15940536655360585 86-87 0.17175961053323968 88-89 0.15926788887654367 90-91 0.18011450390742803 92-93 0.10060241468291585 94-95 0.340344736523407 96-97 0.341800750103202 98-99 0.3806631898145539 100 0.3828628326475489 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8001299.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.200474934671234 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2731797558184 25.987067425400646 2 12.035685159580282 7.991809269244794 3 3.6674466295941484 3.6528290970025497 4 1.6037822505554151 2.129853296409427 5 0.9177525034893237 1.5234909494164532 6 0.5735719737470797 1.1425717162571898 7 0.41582179781153794 0.9663836822872328 8 0.30069708734796685 0.7986628889139856 9 0.2375017689498664 0.7096654374264106 >10 1.5791923976572007 10.313519555698212 >50 0.19336843538699158 4.494192990661768 >100 0.1655840933353443 11.288070672688175 >500 0.022374833262682678 5.088478769346571 >1k 0.011986487103210264 7.313680200741344 >5k 5.327327601342718E-4 1.2662741125012114 >10k+ 0.0015220936003836338 15.333449936004031 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 154605 1.9322487511090387 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 146867 1.8355394542811112 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 64212 0.8025196908651957 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 59226 0.740204809244099 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 51216 0.6400960644015428 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 49626 0.6202242910807357 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 48658 0.6081262554992634 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 46148 0.5767563491878006 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 44724 0.5589592389935685 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 40746 0.5092423117796248 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 39192 0.48982046540193036 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 34283 0.4284679275202689 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 30147 0.37677632094488656 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 23993 0.2998638096139139 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21171 0.264594536462142 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 20680 0.2584580328769116 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 20233 0.25287143999993 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 19557 0.2444228118459265 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 18485 0.23102498731768428 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 17946 0.2242885811416371 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 17268 0.21581495704634962 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 16802 0.20999090272716966 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 16468 0.20581658053273602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14548 0.18182047690006334 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 14499 0.1812080763386045 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 14440 0.18047069607072552 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 14224 0.17777113441204984 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 14055 0.17565897737354896 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 13996 0.17492159710566996 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 13443 0.16801021934063456 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 12819 0.16021148566001595 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 12058 0.150700530001441 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 11880 0.14847589122716198 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 11783 0.14726358807488632 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 11072 0.13837753094841224 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10978 0.13720272170806266 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 10709 0.13384076760536007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10450 0.1306037932090777 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 10353 0.12939149005680203 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 10148 0.12682940607518853 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9243 0.11551874264416316 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8676 0.10843239329013951 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 8069 0.1008461251104352 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 8053 0.10064615758016292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.748579449411902E-5 0.0 1.2497970642017002E-5 0.0 0.0 2 1.2497970642017002E-4 1.2497970642017002E-5 1.2497970642017002E-5 0.0 0.0 3 1.2497970642017002E-4 1.2497970642017002E-5 1.2497970642017002E-5 0.0 0.0 4 1.3747767706218702E-4 2.4995941284034004E-5 1.2497970642017002E-5 0.0 0.0 5 9.623437394353092E-4 2.4995941284034004E-5 1.2497970642017002E-5 3.7493911926051005E-5 0.0 6 0.0016872260366722954 2.4995941284034004E-5 1.2497970642017002E-5 3.7493911926051005E-5 0.0 7 0.0019746793614386863 2.4995941284034004E-5 2.4995941284034004E-5 3.7493911926051005E-5 0.0 8 0.0022371367449210435 2.4995941284034004E-5 2.4995941284034004E-5 4.999188256806801E-5 0.0 9 0.0024246063045512984 3.7493911926051005E-5 2.4995941284034004E-5 4.999188256806801E-5 0.0 10-11 0.0043492937834219165 3.7493911926051005E-5 2.4995941284034004E-5 1.5622463302521254E-4 0.0 12-13 0.009773413042057297 3.7493911926051005E-5 2.4995941284034004E-5 2.3746144219832305E-4 0.0 14-15 0.015991153436460754 6.248985321008501E-5 2.4995941284034004E-5 2.874533247663911E-4 0.0 16-17 0.01850949452082718 7.498782385210201E-5 2.4995941284034004E-5 4.936698403596716E-4 0.0 18-19 0.02010298577768435 7.498782385210201E-5 2.4995941284034004E-5 6.748904146689182E-4 0.0 20-21 0.02523965171155334 7.498782385210201E-5 2.4995941284034004E-5 9.623437394353093E-4 0.0 22-23 0.027314314838128156 7.498782385210201E-5 2.4995941284034004E-5 0.002343369495378188 0.0 24-25 0.03959981997923087 9.373477981512752E-5 2.4995941284034004E-5 0.005642833744870677 0.0 26-27 0.07808107158600122 1.1248173577815303E-4 2.4995941284034004E-5 0.0114668880640506 0.0 28-29 0.08576107454552068 1.1248173577815303E-4 2.4995941284034004E-5 0.035500485608649296 0.0 30-31 0.0873233208757728 1.1248173577815303E-4 2.4995941284034004E-5 0.10308326185535624 0.0 32-33 0.08852312605740643 1.1248173577815303E-4 2.4995941284034004E-5 0.2095472247693781 0.0 34-35 0.0895354616794098 1.6247361834622102E-4 2.4995941284034004E-5 0.3529739358571652 0.0 36-37 0.0912101897454401 1.8746955963025504E-4 2.4995941284034004E-5 0.5626898832302105 0.0 38-39 0.09294740766468045 2.0621651559328053E-4 2.4995941284034004E-5 0.8701649569651128 0.0 40-41 0.09449715602429055 2.1246550091428903E-4 2.4995941284034004E-5 1.3030884110192607 0.0 42-43 0.0984215188058839 2.3121245687731455E-4 2.4995941284034004E-5 1.622249087304449 0.0 44-45 0.10333322126819658 2.4995941284034005E-4 2.4995941284034004E-5 1.9519330548702154 0.0 46-47 0.10536414149752435 2.6245738348235705E-4 2.4995941284034004E-5 2.336308141965448 0.0 48-49 0.10660144059108403 2.6245738348235705E-4 2.4995941284034004E-5 2.686257568927245 0.0 50-51 0.10850738111399161 3.0620028072941656E-4 2.4995941284034004E-5 3.0763442286058797 0.0 52-53 0.10928850427911768 3.124492660504251E-4 2.4995941284034004E-5 3.5385879217862 0.0 54-55 0.11166936768642191 3.186982513714336E-4 2.4995941284034004E-5 3.948990032743433 0.0 56-57 0.11675604173772283 3.249472366924421E-4 2.4995941284034004E-5 4.3823759117113354 0.0 58-59 0.11858699443677832 3.3119622201345056E-4 2.4995941284034004E-5 4.83615847876701 0.0 60-61 0.11973680773584389 3.436941926554676E-4 2.4995941284034004E-5 5.253684432990193 0.0 62-63 0.12053667785693298 3.499431779764761E-4 2.4995941284034004E-5 5.687888929035148 0.0 64-65 0.12104284566793466 3.499431779764761E-4 2.4995941284034004E-5 6.158556254428188 0.0 66-67 0.1219489485394809 3.749391192605101E-4 2.4995941284034004E-5 6.640740459767845 0.0 68-69 0.1230550189412994 3.8743708990252707E-4 2.4995941284034004E-5 7.212616351419938 0.0 70-71 0.12419233426972295 3.9993506054454407E-4 2.4995941284034004E-5 7.916370829286595 0.0 72-73 0.1275980312696726 3.9993506054454407E-4 2.4995941284034004E-5 8.490658829272597 0.0 74-75 0.13099747928430122 3.9993506054454407E-4 2.4995941284034004E-5 9.042163028778202 0.0 76-77 0.13214104359804577 3.9993506054454407E-4 2.4995941284034004E-5 9.656163330479213 0.0 78-79 0.13298465661638192 3.9993506054454407E-4 2.4995941284034004E-5 10.246561214622776 0.0 80-81 0.1344531681668189 3.9993506054454407E-4 2.4995941284034004E-5 10.91430279008446 0.0 82-83 0.1351718014787349 3.9993506054454407E-4 2.4995941284034004E-5 11.69435737872063 0.0 84-85 0.13774013444566938 3.9993506054454407E-4 2.4995941284034004E-5 12.412896955856793 0.0 86-87 0.1419332035960661 4.249310018285781E-4 2.4995941284034004E-5 13.122994153824273 0.0 88 0.14308926588045265 4.499269431126121E-4 2.4995941284034004E-5 13.69632605905616 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACAC 8985 0.0 27.623453 3 ATCTACA 8230 0.0 27.351135 2 TACACTC 9275 0.0 26.479443 5 CTACACT 11435 0.0 22.397434 4 CACTCTT 11470 0.0 21.36251 7 AGTACTT 27600 0.0 19.57065 12-13 ACTCTTT 14445 0.0 19.271921 8 ACACTCT 13005 0.0 18.983334 6 GAGTACT 26480 0.0 18.773994 12-13 GTATAAG 2525 0.0 18.095562 1 CGTTAAC 545 0.0 17.286028 1 CATGGGT 8345 0.0 16.86865 4 TATACCG 1395 0.0 16.863512 5 CATGGGG 16115 0.0 16.652523 4 ATTGCGT 1210 0.0 16.322529 6 GTACTTT 30885 0.0 16.185337 14-15 TACTTTT 33760 0.0 16.129047 14-15 AGTACTC 2610 0.0 15.863331 5 ATGGGTA 5475 0.0 15.640099 5 CTAACGC 1125 0.0 15.489401 3 >>END_MODULE