FastQCFastQC Report
Fri 27 May 2016
ERR765262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6702455
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA96980.14469325045822762No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86800.12950478593291564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG26000.020.5670491
GTACTAG17100.020.1225511
GTATCAA217950.019.1374051
GTACATG339600.018.6627481
TCTAACG9350.018.4008372
TACATGG337750.018.1179052
TATAAGG27450.017.6172642
GTATATA20450.016.8261951
CATGGGG138900.016.770554
ACATGGG338100.016.7238333
TAGTACT24400.016.1972354
CTAGTAC25500.016.0468563
AACGCAG242500.015.91366
GTATTAT19400.015.8193861
TAGGACT27450.015.7526064
CAACGCA243550.015.6926215
GTGATCG16600.015.6846768
ATAAGGT23550.015.5985283
GTACATA18250.015.5422691
ATCAACG245350.015.52191453
Produced by FastQC (version 0.11.5)