##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6702455 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.993783621076158 33.0 33.0 33.0 27.0 33.0 2 31.004361536183385 33.0 33.0 33.0 27.0 33.0 3 30.98430008108969 33.0 33.0 33.0 27.0 33.0 4 34.55635151597437 37.0 37.0 37.0 27.0 37.0 5 34.610480786517776 37.0 37.0 37.0 27.0 37.0 6 34.56352605127524 37.0 37.0 37.0 27.0 37.0 7 34.44870036426951 37.0 37.0 37.0 27.0 37.0 8 34.42413608148059 37.0 37.0 37.0 27.0 37.0 9 34.38261413168757 37.0 37.0 37.0 27.0 37.0 10-11 34.337441803040825 37.0 37.0 37.0 27.0 37.0 12-13 34.24496785133209 37.0 35.0 37.0 27.0 37.0 14-15 35.52101946227166 37.0 37.0 40.0 27.0 40.0 16-17 35.54557665810513 37.0 37.0 40.0 27.0 40.0 18-19 35.535700053189466 37.0 37.0 40.0 27.0 40.0 20-21 35.5203488870869 37.0 35.0 40.0 27.0 40.0 22-23 35.48342480479168 37.0 33.0 40.0 27.0 40.0 24-25 35.5318142382157 37.0 33.0 40.0 27.0 40.0 26-27 35.45980062529327 37.0 33.0 40.0 27.0 40.0 28-29 35.44391465515248 37.0 33.0 40.0 27.0 40.0 30-31 35.349087759634344 37.0 33.0 40.0 27.0 40.0 32-33 35.264946202548174 37.0 33.0 40.0 27.0 40.0 34-35 35.12452638622713 37.0 33.0 40.0 27.0 40.0 36-37 34.923933319955154 37.0 33.0 40.0 27.0 40.0 38-39 34.640950741183644 37.0 33.0 40.0 27.0 40.0 40-41 34.760553185362674 37.0 33.0 40.0 27.0 40.0 42-43 34.67280869472455 37.0 33.0 40.0 27.0 40.0 44-45 34.588299809547394 37.0 33.0 40.0 27.0 40.0 46-47 34.48730465180296 37.0 33.0 40.0 24.5 40.0 48-49 34.342606030178494 37.0 33.0 40.0 22.0 40.0 50-51 34.156202167713175 37.0 33.0 40.0 22.0 40.0 52-53 34.01381971232929 37.0 33.0 40.0 22.0 40.0 54-55 33.810062506947084 37.0 33.0 38.5 22.0 40.0 56-57 33.59774903374958 37.0 33.0 37.0 22.0 40.0 58-59 33.3789489821267 37.0 33.0 37.0 22.0 40.0 60-61 33.16234476770079 37.0 33.0 37.0 22.0 40.0 62-63 32.937905812124065 37.0 33.0 37.0 22.0 40.0 64-65 32.71254913311615 37.0 33.0 37.0 22.0 40.0 66-67 32.494384445699374 37.0 33.0 37.0 22.0 40.0 68-69 32.30712365842068 33.0 33.0 37.0 22.0 38.5 70-71 32.05267950325664 33.0 33.0 37.0 22.0 37.0 72-73 31.711204103571006 33.0 27.0 37.0 22.0 37.0 74-75 31.47808937173021 33.0 27.0 37.0 22.0 37.0 76-77 31.29303799876314 33.0 27.0 37.0 22.0 37.0 78-79 31.03508617364831 33.0 27.0 37.0 22.0 37.0 80-81 30.81665404691266 33.0 27.0 37.0 22.0 37.0 82-83 30.697054288316743 33.0 27.0 37.0 18.5 37.0 84-85 30.561324246115788 33.0 27.0 37.0 15.0 37.0 86-87 30.28772912313473 33.0 27.0 37.0 15.0 37.0 88-89 30.1369367642155 33.0 27.0 37.0 15.0 37.0 90-91 29.991580622324207 33.0 27.0 37.0 15.0 37.0 92-93 29.73997005574823 33.0 27.0 37.0 15.0 37.0 94-95 29.603910059821366 33.0 27.0 37.0 15.0 37.0 96-97 29.404827111856775 33.0 27.0 37.0 15.0 37.0 98-99 28.385355291456637 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33756183436958764 1101 2 0.32317515819423903 1101 3 0.37853970460794883 1101 4 0.42835465928031624 1101 5 0.3983798441655253 1101 6 0.4933613491838287 1101 7 0.4126938224638934 1101 8 0.513033628920482 1101 9 0.39251158619397586 1101 10-11 0.3570401942335124 1101 12-13 0.5185007994827728 1101 14-15 0.7196440260012267 1101 16-17 0.6362124132429727 1101 18-19 0.6205288676814575 1101 20-21 0.6290685909822571 1101 22-23 0.6573233577769315 1101 24-25 0.6093984189052506 1101 26-27 0.6546633265453252 1101 28-29 0.5546326814801574 1101 30-31 0.7933954936671554 1101 32-33 0.8487683712970551 1101 34-35 0.9254437428793878 1101 36-37 1.0267970102651347 1101 38-39 1.0195841826680123 1101 40-41 0.7463294421996522 1101 42-43 0.701118418911733 1101 44-45 0.6003689668278227 1101 46-47 0.6419142294482754 1101 48-49 0.5495754444566003 1101 50-51 0.5322757683714272 1101 52-53 0.6109431390082491 1101 54-55 0.49151363229056244 1101 56-57 0.6189359582783354 1101 58-59 0.5336943769761859 1101 60-61 0.39380523660658184 1101 62-63 0.51871460489496 1101 64-65 0.6038462978609971 1101 66-67 0.5645333402642123 1101 68-69 0.6446889261063262 1101 70-71 0.6639457795000183 1101 72-73 0.36182785032965015 1101 74-75 0.4344315793081144 1101 76-77 0.4409231593190661 1101 78-79 0.4866510201188916 1101 80-81 0.5358644659863145 1101 82-83 0.6103342197498307 1101 84-85 0.5179932806806811 1101 86-87 0.43992151864259554 1101 88-89 0.3603050062212887 1101 90-91 0.3289618272425905 1101 92-93 0.4154047546885131 1101 94-95 0.4397936232135322 1101 96-97 0.4098983728272394 1101 98-99 0.38271764971550226 1102 1 0.29748998255186265 1102 2 0.3350487455311928 1102 3 0.32371954123813396 1102 4 0.29415598621213945 1102 5 0.3529156251142709 1102 6 0.45196310536510964 1102 7 0.47970466119891597 1102 8 0.49010864566186285 1102 9 0.38885823770847594 1102 10-11 0.338957660507603 1102 12-13 0.5222285313526385 1102 14-15 0.6845641257561184 1102 16-17 0.4640203231787581 1102 18-19 0.4807630562096463 1102 20-21 0.5348871316364736 1102 22-23 0.5797466952845554 1102 24-25 0.4520933832748426 1102 26-27 0.5336549114130733 1102 28-29 0.43000553610540493 1102 30-31 0.6231232757960683 1102 32-33 0.7121138598731349 1102 34-35 0.7878146108763318 1102 36-37 0.906577741227089 1102 38-39 0.8463852829687681 1102 40-41 0.6657795431132953 1102 42-43 0.4821337539696984 1102 44-45 0.47755327372070866 1102 46-47 0.41129687155688543 1102 48-49 0.3252562846388898 1102 50-51 0.3294235443854845 1102 52-53 0.4653711934201752 1102 54-55 0.27711756682909083 1102 56-57 0.40665074937522405 1102 58-59 0.3267253882020995 1102 60-61 0.3175947405637771 1102 62-63 0.38527795199834003 1102 64-65 0.4313170487264486 1102 66-67 0.4830160476630354 1102 68-69 0.5402899660962319 1102 70-71 0.5878536435046371 1102 72-73 0.402818879555749 1102 74-75 0.4170842609305687 1102 76-77 0.3944087356716288 1102 78-79 0.40588985155890356 1102 80-81 0.3164609244921195 1102 82-83 0.31085150164768294 1102 84-85 0.5005162660492495 1102 86-87 0.4611558016391726 1102 88-89 0.34416588267385606 1102 90-91 0.32992563747374604 1102 92-93 0.3688018658173071 1102 94-95 0.4899373035809056 1102 96-97 0.1978028080482197 1102 98-99 0.30965148042722035 1103 1 0.2689642110401351 1103 2 0.30376544699144503 1103 3 0.20411888582026094 1103 4 0.2640590212112741 1103 5 0.22552466591523057 1103 6 0.43557510848422965 1103 7 0.28401015942923635 1103 8 0.36404318496970944 1103 9 0.2660769446339799 1103 10-11 0.3613055068326929 1103 12-13 0.40199245997855826 1103 14-15 0.5755888759606762 1103 16-17 0.33639436606218 1103 18-19 0.33246722940084084 1103 20-21 0.4147771843741239 1103 22-23 0.3891725061954219 1103 24-25 0.284545638482804 1103 26-27 0.4256330363376293 1103 28-29 0.39306633569928096 1103 30-31 0.6465740260554611 1103 32-33 0.6549508883110562 1103 34-35 0.768555277136592 1103 36-37 0.880972404319941 1103 38-39 0.8495922929680901 1103 40-41 0.6136526905511417 1103 42-43 0.4961831250015081 1103 44-45 0.3935742984685717 1103 46-47 0.42269881282568633 1103 48-49 0.3189382569676198 1103 50-51 0.3410683915245656 1103 52-53 0.3679455015739279 1103 54-55 0.21486318622406486 1103 56-57 0.4090449183968161 1103 58-59 0.33424340902949723 1103 60-61 0.24331967646693897 1103 62-63 0.38491016891342866 1103 64-65 0.4607851885129861 1103 66-67 0.5062343875507835 1103 68-69 0.513943836364092 1103 70-71 0.5008123826947681 1103 72-73 0.31925476947348486 1103 74-75 0.4385417383405219 1103 76-77 0.34164356553931796 1103 78-79 0.33032145983820627 1103 80-81 0.20170423466645104 1103 82-83 0.347814032155064 1103 84-85 0.4661360219621038 1103 86-87 0.38385219320798925 1103 88-89 0.37742302871856026 1103 90-91 0.2584967191640253 1103 92-93 0.21115813104169945 1103 94-95 0.3943745322723835 1103 96-97 0.2921770145282885 1103 98-99 0.3378655745365471 1104 1 0.19705359508418496 1104 2 0.22612554431504606 1104 3 0.22648666658604455 1104 4 0.2130998715122061 1104 5 0.16605866514688472 1104 6 0.3456942018380502 1104 7 0.233792461900741 1104 8 0.3186455668367856 1104 9 0.2256031323660821 1104 10-11 0.27075229899557485 1104 12-13 0.2807362171817829 1104 14-15 0.3614199682900363 1104 16-17 0.18893502427705755 1104 18-19 0.3302563189129444 1104 20-21 0.24807594221764617 1104 22-23 0.28816789613011906 1104 24-25 0.16438620705752527 1104 26-27 0.28250843651691326 1104 28-29 0.18169676213031494 1104 30-31 0.442339809126274 1104 32-33 0.4199630537611725 1104 34-35 0.5042032474272773 1104 36-37 0.5785169042559133 1104 38-39 0.5359157658839564 1104 40-41 0.3579912742366389 1104 42-43 0.2694473073552004 1104 44-45 0.25937785846344497 1104 46-47 0.19021642071402312 1104 48-49 -0.06018390976307586 1104 50-51 -0.07040554880069294 1104 52-53 0.06932147801136779 1104 54-55 -0.061722164012849134 1104 56-57 0.18352820691007565 1104 58-59 0.08660542721364806 1104 60-61 -0.029339434814914966 1104 62-63 0.14948565873287123 1104 64-65 0.14960578013803882 1104 66-67 0.08845170090716437 1104 68-69 0.19654916355067087 1104 70-71 0.1698212186532828 1104 72-73 0.01948783388634112 1104 74-75 -0.008774819477377349 1104 76-77 0.09159234259143645 1104 78-79 0.12271869379902256 1104 80-81 0.03607995498915528 1104 82-83 0.14449094341130575 1104 84-85 0.22368308304546858 1104 86-87 0.12085372989642806 1104 88-89 0.07356081844835671 1104 90-91 -0.08776784736177845 1104 92-93 -0.044888097668764004 1104 94-95 0.0970240392515116 1104 96-97 -0.060186924516404616 1104 98-99 -0.08678867056525874 1105 1 0.0987454581588878 1105 2 0.035970620457320024 1105 3 0.08362973407976071 1105 4 0.03259711909370111 1105 5 -0.050176288528902546 1105 6 0.13021517374199476 1105 7 0.06008972435866866 1105 8 0.13499539801183147 1105 9 0.03794524387097198 1105 10-11 0.061131284355212756 1105 12-13 0.11291932158802354 1105 14-15 0.20451980140057913 1105 16-17 0.003222009399479475 1105 18-19 0.17592500434458458 1105 20-21 0.11977887705029389 1105 22-23 0.07771772669024557 1105 24-25 -0.054009020177254285 1105 26-27 0.0879716039464924 1105 28-29 0.011451346682314067 1105 30-31 0.2657439038801357 1105 32-33 0.339260406640868 1105 34-35 0.36027819625341095 1105 36-37 0.5454892666824875 1105 38-39 0.42142264247580385 1105 40-41 0.23106678811544867 1105 42-43 0.0632203223520662 1105 44-45 0.06607220285805937 1105 46-47 0.05615613028939492 1105 48-49 -0.14595987630264773 1105 50-51 -0.0483911274738702 1105 52-53 -0.049663918855166855 1105 54-55 -0.16896815988633307 1105 56-57 0.029659757501086403 1105 58-59 -0.12445426493080447 1105 60-61 -0.16364081907543948 1105 62-63 -0.07715702153269177 1105 64-65 0.07757562112723804 1105 66-67 -0.0065623991093772815 1105 68-69 0.036214682849980306 1105 70-71 0.08536076191335695 1105 72-73 -0.13286863471295263 1105 74-75 0.05089239046073146 1105 76-77 -0.00935286118962253 1105 78-79 -0.09988064076939906 1105 80-81 -0.22393314412773435 1105 82-83 -0.05556583041793317 1105 84-85 -0.1463405990480524 1105 86-87 -0.18494093924233468 1105 88-89 -0.2728729467942941 1105 90-91 -0.19513728413123843 1105 92-93 -0.2855307832314651 1105 94-95 -0.19853915813468248 1105 96-97 -0.369416606651491 1105 98-99 -0.3807169833681101 1106 1 0.07670746547335838 1106 2 0.10364681585653202 1106 3 0.028398151982848674 1106 4 -0.1187298693340395 1106 5 0.06235659600719856 1106 6 0.15978173954854924 1106 7 0.08153870841310606 1106 8 0.04656322347931763 1106 9 0.11308910172456166 1106 10-11 0.14530164472606089 1106 12-13 0.06858512615572465 1106 14-15 0.1685290973676814 1106 16-17 -0.018904726602833932 1106 18-19 0.1861707416607956 1106 20-21 0.11410079556457475 1106 22-23 0.024589375464785235 1106 24-25 0.09928691774293696 1106 26-27 0.14185889526856243 1106 28-29 0.09920360210242762 1106 30-31 0.27512749621585186 1106 32-33 0.3363286609492704 1106 34-35 0.42887278168441156 1106 36-37 0.48869561908251313 1106 38-39 0.4286804902754824 1106 40-41 0.24220519936464768 1106 42-43 0.11617141700389055 1106 44-45 0.12227180811570548 1106 46-47 0.07638533200223918 1106 48-49 -0.0507903645584733 1106 50-51 -0.09908716090389902 1106 52-53 0.05433499846440526 1106 54-55 -0.049400452246345594 1106 56-57 -0.1286943838325314 1106 58-59 -0.12427799301366349 1106 60-61 -0.11499528678128002 1106 62-63 0.027724713952530067 1106 64-65 0.0633009915232634 1106 66-67 0.031122107342781646 1106 68-69 -0.005365865483689447 1106 70-71 0.046231258253293106 1106 72-73 -0.19033653734989642 1106 74-75 -0.024137716561860145 1106 76-77 -0.03820323663546432 1106 78-79 -0.03148330872101468 1106 80-81 0.07792215668031588 1106 82-83 -0.027800219742900367 1106 84-85 -0.03888441014241906 1106 86-87 -0.009507282258855554 1106 88-89 -0.21524391938589105 1106 90-91 -0.244831250132318 1106 92-93 -0.21135523841412862 1106 94-95 -0.0070433197071295695 1106 96-97 -0.22917889327343843 1106 98-99 -0.13121319042528512 1107 1 0.06476358476494326 1107 2 0.096559321946728 1107 3 0.0274418864718875 1107 4 -0.0409007727943802 1107 5 -0.01349384307253132 1107 6 0.07570873923908295 1107 7 -0.023657126826904573 1107 8 0.045952068406336366 1107 9 -0.11269823677991297 1107 10-11 0.035491719353501594 1107 12-13 0.04521852702932705 1107 14-15 0.14910919577934578 1107 16-17 -0.043002563754747314 1107 18-19 0.14136360567295725 1107 20-21 0.06407109148120327 1107 22-23 -0.10664541110644876 1107 24-25 -0.002025473511992004 1107 26-27 0.03187223966024533 1107 28-29 0.015327068885568451 1107 30-31 0.18421617355397046 1107 32-33 0.24947972522159745 1107 34-35 0.2599357405913665 1107 36-37 0.3870648793837219 1107 38-39 0.28063539703475016 1107 40-41 0.11140112701048821 1107 42-43 0.07816581131768174 1107 44-45 -0.001723335461072395 1107 46-47 -0.08325075969472095 1107 48-49 -0.1659346273687916 1107 50-51 -0.10649291219939272 1107 52-53 -0.03588903994727133 1107 54-55 -0.1079553539172835 1107 56-57 -0.024821842241301795 1107 58-59 -0.06585720635818859 1107 60-61 -0.1561253347931526 1107 62-63 -0.09080914800136242 1107 64-65 -0.017302028049201112 1107 66-67 -0.04473123649234623 1107 68-69 0.07694177655989876 1107 70-71 0.0058993213818823165 1107 72-73 -0.30268134897140797 1107 74-75 -0.02502442274206018 1107 76-77 -0.08654582873246497 1107 78-79 -0.08476392581271597 1107 80-81 -0.07340942599305578 1107 82-83 -0.15931913965322053 1107 84-85 -0.060401062522366544 1107 86-87 -0.22583084116007157 1107 88-89 -0.3196928136201791 1107 90-91 -0.29303251540735076 1107 92-93 -0.370455930876858 1107 94-95 -0.25346563082195317 1107 96-97 -0.36179785042837054 1107 98-99 -0.3423270158790608 1108 1 0.15593209827344978 1108 2 0.1281895105222084 1108 3 0.05562628528454994 1108 4 0.004591472003433239 1108 5 0.012154444468677639 1108 6 0.20647795892303833 1108 7 0.027160445901486696 1108 8 0.013699466800439097 1108 9 0.13131595811270103 1108 10-11 0.06834165094316802 1108 12-13 -0.03018821689058626 1108 14-15 -0.017309205372072256 1108 16-17 -0.08061594943519879 1108 18-19 0.2213958446612807 1108 20-21 0.11971476927052294 1108 22-23 0.03294509236418719 1108 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-0.4786635123926999 2216 10-11 -0.6285323139071437 2216 12-13 -0.6261716515424638 2216 14-15 -0.7011985971211914 2216 16-17 -0.7123734680980363 2216 18-19 -0.6329510088036017 2216 20-21 -0.4258898467403114 2216 22-23 -0.4091093632153786 2216 24-25 -0.5606018769116687 2216 26-27 -0.5594916724193197 2216 28-29 -0.5815802702948574 2216 30-31 -0.44150919639635333 2216 32-33 -0.3323046021863689 2216 34-35 -0.5025824073364475 2216 36-37 -0.2908074187968239 2216 38-39 -8.620244152497111 2216 40-41 -2.1698587064642822 2216 42-43 -0.7948235645634796 2216 44-45 -0.8696344464443442 2216 46-47 -0.5507142176721729 2216 48-49 -0.3615550540404229 2216 50-51 -0.36182530089296705 2216 52-53 -0.44103087006195096 2216 54-55 -0.3509645170787792 2216 56-57 -0.21964207253668633 2216 58-59 -0.2830711380524491 2216 60-61 -0.2997656604696104 2216 62-63 -0.35073082939841527 2216 64-65 -0.46736737070118295 2216 66-67 -0.28483295162516953 2216 68-69 -0.29369067982002406 2216 70-71 -0.12089096369220798 2216 72-73 -0.13766195650190127 2216 74-75 -0.21354637550470912 2216 76-77 -0.2942083540575453 2216 78-79 -0.2864991115196389 2216 80-81 -0.30201395781011087 2216 82-83 -0.4366594611456307 2216 84-85 -0.33202922416048253 2216 86-87 -0.13734051371067224 2216 88-89 -0.10079436323356106 2216 90-91 -0.1500927418773088 2216 92-93 -0.08974139024812544 2216 94-95 -0.011755433471815735 2216 96-97 0.008843944789258984 2216 98-99 0.05557567352193615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 234.0 8 1359.0 9 2598.0 10 4549.0 11 8836.0 12 15441.0 13 23643.0 14 29848.0 15 33389.0 16 37079.0 17 41162.0 18 45503.0 19 50285.0 20 56174.0 21 62798.0 22 70446.0 23 78616.0 24 89255.0 25 100662.0 26 112241.0 27 128629.0 28 150525.0 29 182043.0 30 223739.0 31 278512.0 32 354688.0 33 457321.0 34 602148.0 35 824250.0 36 1144452.0 37 1190512.0 38 301306.0 39 209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39813471332519 18.06767818657492 12.109264441163722 25.42492265893617 2 16.24763178523929 20.52269771031045 37.392891342173876 25.836779162276386 3 18.255973450436144 25.34703286212791 28.625979199439694 27.771014487996247 4 13.25491927957741 17.009066081010616 36.1055165607229 33.63049807868907 5 14.903978933242495 36.94951584744118 33.24303358801382 14.903471631302498 6 32.68864915915138 36.08829898895255 17.357848728562892 13.865203123333167 7 28.68187919783517 31.25249070745118 21.745123733795573 18.320506360918078 8 26.975458992264773 33.26173767671696 20.107005567363004 19.655797763655258 9 27.02218813852536 15.28812949881797 18.95426377349792 38.73541858915875 10-11 25.554535696925335 25.310319175633282 27.066675877223478 22.068469250217905 12-13 26.369926397804267 23.082314905916114 26.537081704233657 24.01067699204597 14-15 24.241793971831623 24.250790926295398 23.65802143393848 27.849393667934496 16-17 24.507860747883516 26.667124210969202 24.381183142426703 24.443831898720582 18-19 24.849670754969637 26.12251779385315 25.411479823437833 23.616331627739388 20-21 25.891177188060194 25.569757648503423 24.516374970066938 24.022690193369446 22-23 25.263019453629727 25.365847508620355 24.440596432558774 24.930536605191143 24-25 24.85162931914316 25.746825514587307 24.497441992854636 24.9041031734149 26-27 25.046060794056235 25.766054211225175 24.355445423936484 24.832439570782107 28-29 24.766170171179787 25.601404590357653 24.82594060853646 24.8064846299261 30-31 25.049964422496323 25.619868435599948 24.65820157322841 24.671965568675326 32-33 24.732228320951073 25.651093410814052 24.371081486724492 25.245596781510383 34-35 25.11085304644739 25.598040240332853 24.75126835997171 24.539838353248054 36-37 25.06562157541017 25.664470204529838 24.408048107817372 24.861860112242624 38-39 25.057337352334763 25.369936581447256 24.65989839834602 24.91282766787196 40-41 24.987983476244732 25.54989350931656 24.71712584595917 24.744997168479532 42-43 25.239780303807386 25.5369259317972 24.56207909435933 24.661214670036088 44-45 24.93561127494413 25.62842463160324 24.443806369741115 24.992157723711514 46-47 24.699786554006494 25.6944678606075 24.81767719553509 24.788068389850917 48-49 25.286786963988835 25.68259751025334 24.54176824023577 24.488847285522052 50-51 24.601234824580622 25.9296973722659 24.561435704577587 24.907632098575895 52-53 24.762752427679505 25.603237448096323 24.497214782766783 25.13679534145739 54-55 24.862389975016615 25.43186041532543 24.658143918907328 25.04760569075063 56-57 24.901809873298948 25.227982595268983 24.502909802645473 25.367297728786593 58-59 24.727107414687218 25.253183386264194 24.914658123726028 25.10505107532256 60-61 24.76083535803025 25.408285799052244 24.686675836954088 25.144203005963423 62-63 24.524759815548126 25.490257074536743 24.577255521224842 25.40772758869029 64-65 24.52660029886325 25.130630514025842 24.70052316361767 25.642246023493236 66-67 24.91989782132518 25.244581138900422 24.673955225138783 25.161565814635612 68-69 24.807900241958937 24.945321637383273 24.805319068145526 25.441459052512265 70-71 24.3350400469082 25.033127799115324 24.794573329687903 25.837258824288572 72-73 24.783733581346826 25.170591959639072 24.81092564392035 25.23474881509375 74-75 25.016264804216963 24.896762041345017 24.76063825620709 25.32633489823093 76-77 24.720706440603923 25.106006075990766 24.857723583300782 25.315563900104525 78-79 24.729740536592004 25.059537947925893 24.925527210356574 25.28519430512553 80-81 24.473756257968162 24.904635689460054 25.167487137175854 25.45412091539593 82-83 24.63920191369313 25.11504404445371 24.77943457249032 25.46631946936284 84-85 24.63147260712886 25.116133443761125 24.672763669355795 25.579630279754216 86-87 24.48876568361891 24.862486954407004 24.880540040925304 25.76820732104878 88-89 24.57672219757122 24.575274966452586 25.0513990845741 25.7966037514021 90-91 24.834332102682392 24.80824449097825 24.800344353490495 25.557079052848863 92-93 24.525946089902877 24.84638091564956 24.83827940657565 25.789393587871906 94-95 24.138110587836845 24.920540309483616 25.221743376121136 25.719605726558402 96-97 24.688632850742135 24.737743176495854 24.87307073371483 25.70055323904718 98-99 24.46552177929928 24.772893569580123 24.7648662875641 25.996718363556496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 976.0 1 991.5 2 991.5 3 937.0 4 841.0 5 740.5 6 665.5 7 607.0 8 584.0 9 563.5 10 530.5 11 528.0 12 538.0 13 576.5 14 622.0 15 694.0 16 824.5 17 982.0 18 1232.0 19 1627.0 20 2224.0 21 3159.0 22 4456.0 23 6167.5 24 8515.5 25 11779.0 26 15712.5 27 19613.0 28 24462.5 29 30678.0 30 36780.0 31 43430.0 32 50835.0 33 59383.0 34 68507.5 35 77693.5 36 86506.0 37 96329.0 38 109294.5 39 119281.5 40 128409.0 41 139933.0 42 155904.0 43 177028.0 44 198111.5 45 218605.0 46 248097.5 47 273770.5 48 287245.5 49 299472.0 50 314752.5 51 316457.5 52 310587.5 53 314214.5 54 308347.0 55 296574.5 56 272940.5 57 252231.5 58 237548.0 59 211051.0 60 178783.0 61 140870.0 62 115388.5 63 94367.0 64 70509.0 65 52564.0 66 37828.0 67 28749.0 68 22888.0 69 19702.5 70 17421.5 71 15139.0 72 14163.0 73 12189.5 74 8797.0 75 6431.5 76 4682.5 77 3203.0 78 2151.0 79 1424.0 80 996.0 81 716.0 82 515.0 83 379.5 84 266.0 85 186.5 86 139.5 87 101.0 88 67.0 89 47.0 90 32.5 91 25.0 92 17.5 93 12.0 94 14.0 95 12.5 96 7.0 97 4.5 98 5.5 99 6.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0022230660258069617 3 0.006326040234511086 4 0.0 5 0.004953408862871888 6 0.0 7 6.266360609657207E-4 8 0.0 9 0.0 10-11 1.4919906213469542E-5 12-13 8.205948417408248E-5 14-15 0.0027900224619188045 16-17 1.1189929660102157E-4 18-19 0.0 20-21 0.0 22-23 5.967962485387817E-5 24-25 0.0010369334818361331 26-27 0.0025587639156100265 28-29 0.0021633864009530835 30-31 0.002775102555705335 32-33 8.42974701061029E-4 34-35 7.459953106734771E-6 36-37 1.4919906213469543E-4 38-39 0.05881427029349694 40-41 0.003364438851137382 42-43 2.61098358735717E-4 44-45 7.459953106734771E-6 46-47 8.50434654167764E-4 48-49 8.951943728081725E-5 50-51 6.788557327128641E-4 52-53 2.312585463087779E-4 54-55 0.0 56-57 3.655377022300038E-4 58-59 1.4919906213469542E-5 60-61 1.7157892145489974E-4 62-63 2.9839812426939084E-5 64-65 8.728145134879682E-4 66-67 0.0011637526846506243 68-69 0.0011637526846506243 70-71 3.7299765533673856E-5 72-73 0.001961967667071245 74-75 0.0014845306682402196 76-77 1.3427915592122586E-4 78-79 1.044393434942868E-4 80-81 0.0 82-83 3.282379366963299E-4 84-85 5.371166236849034E-4 86-87 0.0 88-89 1.4919906213469542E-5 90-91 5.967962485387817E-4 92-93 0.0 94-95 0.0 96-97 0.002961601383373704 98-99 0.004640090832389028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6702455.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.08145205641732 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85159378163817 36.18284628089457 2 12.873814094717307 12.122357221673248 3 4.15758969394537 5.872360795372311 4 1.8537375697864358 3.4910662606831853 5 1.006668747754203 2.3697713192041583 6 0.6572666549509132 1.8567041102011923 7 0.4420340618879512 1.4568123844456684 8 0.3226203454059595 1.2151547459724383 9 0.22600405382845068 0.9576539122392144 >10 1.3660606800568602 11.938728813107021 >50 0.12324985898207529 4.057549246961925 >100 0.10180564467190305 9.735758024596153 >500 0.011959066053520165 3.858810365354376 >1k 0.00553179493455248 4.607752700297372 >5k 6.395138650179353E-5 0.27667381899722787 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9698 0.14469325045822762 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8680 0.12950478593291564 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.4919906213469542E-5 0.0 0.0 0.0 5 0.0 4.4759718640408625E-5 0.0 2.9839812426939084E-5 0.0 6 0.0 4.4759718640408625E-5 0.0 2.9839812426939084E-5 0.0 7 0.0 4.4759718640408625E-5 0.0 2.9839812426939084E-5 1.4919906213469542E-5 8 0.0 4.4759718640408625E-5 0.0 2.9839812426939084E-5 2.9839812426939084E-5 9 0.0 7.459953106734771E-5 0.0 2.9839812426939084E-5 2.9839812426939084E-5 10-11 0.0 7.459953106734771E-5 0.0 2.9839812426939084E-5 2.9839812426939084E-5 12-13 0.0 8.205948417408248E-5 0.0 2.9839812426939084E-5 3.7299765533673856E-5 14-15 7.459953106734771E-6 8.951943728081725E-5 0.0 2.9839812426939084E-5 6.713957796061294E-5 16-17 1.4919906213469542E-5 8.951943728081725E-5 0.0 2.9839812426939084E-5 7.459953106734771E-5 18-19 1.4919906213469542E-5 1.0443934349428679E-4 0.0 6.713957796061294E-5 7.459953106734771E-5 20-21 1.4919906213469542E-5 1.4919906213469543E-4 0.0 1.790388745616345E-4 8.951943728081725E-5 22-23 2.9839812426939084E-5 1.4919906213469543E-4 0.0 6.788557327128641E-4 1.0443934349428679E-4 24-25 2.9839812426939084E-5 1.4919906213469543E-4 0.0 0.0016859494021220583 1.4919906213469543E-4 26-27 4.4759718640408625E-5 1.4919906213469543E-4 0.0 0.0032450796014296254 1.4919906213469543E-4 28-29 4.4759718640408625E-5 1.4919906213469543E-4 0.0 0.009556199929727241 1.4919906213469543E-4 30-31 5.967962485387817E-5 1.790388745616345E-4 0.0 0.02740786771414355 1.566590152414302E-4 32-33 7.459953106734771E-5 1.790388745616345E-4 0.0 0.05985120377533307 1.9395878077510404E-4 34-35 7.459953106734771E-5 1.9395878077510406E-4 0.0 0.10292497301361964 2.088786869885736E-4 36-37 8.951943728081725E-5 2.2379859320204314E-4 0.0 0.16496194304922598 2.088786869885736E-4 38-39 8.951943728081725E-5 2.4617845252224743E-4 0.0 0.25969588755165085 2.088786869885736E-4 40-41 8.951943728081725E-5 2.7601826494918656E-4 0.0 0.37509390215973104 2.088786869885736E-4 42-43 8.951943728081725E-5 2.834782180559213E-4 0.0 0.48219644891312213 2.088786869885736E-4 44-45 9.697939038755203E-5 2.9093817116265607E-4 0.0 0.606770205842486 2.2379859320204314E-4 46-47 1.044393434942868E-4 2.9839812426939085E-4 0.0 0.7463310085632802 2.3871849941551268E-4 48-49 1.044393434942868E-4 3.282379366963299E-4 0.0 0.8896814674622955 2.536384056289822E-4 50-51 1.044393434942868E-4 3.5061779601653427E-4 0.0 1.0461017642043102 2.536384056289822E-4 52-53 1.044393434942868E-4 3.58077749123269E-4 0.0 1.2049167058935866 2.536384056289822E-4 54-55 1.044393434942868E-4 3.804576084434733E-4 0.0 1.3669692672311862 2.7601826494918656E-4 56-57 1.044393434942868E-4 3.879175615502081E-4 0.0 1.5469331759780558 2.9839812426939085E-4 58-59 1.1189929660102157E-4 3.879175615502081E-4 0.0 1.74308518296654 2.9839812426939085E-4 60-61 1.1935924970775634E-4 3.879175615502081E-4 0.0 1.9442651983489632 2.9839812426939085E-4 62-63 1.1935924970775634E-4 3.879175615502081E-4 0.0 2.1502643434383373 3.282379366963299E-4 64-65 1.1935924970775634E-4 3.879175615502081E-4 0.0 2.3640964392897823 3.356978898030647E-4 66-67 1.1935924970775634E-4 3.879175615502081E-4 0.0 2.603777272656064 3.58077749123269E-4 68-69 1.1935924970775634E-4 4.102974208704124E-4 0.0 2.861354533525402 3.7299765533673856E-4 70-71 1.1935924970775634E-4 4.401372332973515E-4 0.0 3.1477346733398432 3.7299765533673856E-4 72-73 1.342791559212259E-4 4.475971864040863E-4 0.0 3.4332942183125437 4.326772801906167E-4 74-75 1.4919906213469543E-4 4.475971864040863E-4 0.0 3.718130147833891 4.326772801906167E-4 76-77 1.4919906213469543E-4 4.6251709261755584E-4 0.0 4.025994952595728 4.326772801906167E-4 78-79 1.4919906213469543E-4 4.923569050444949E-4 0.0 4.351040029362375 4.326772801906167E-4 80-81 1.4919906213469543E-4 5.072768112579644E-4 0.0 4.703381074546565 4.475971864040863E-4 82-83 1.4919906213469543E-4 5.147367643646992E-4 0.0 5.078579416049791 4.8489695193776013E-4 84-85 1.4919906213469543E-4 5.22196717471434E-4 0.0 5.457753912558905 4.923569050444949E-4 86-87 1.4919906213469543E-4 5.445765767916383E-4 0.0 5.849863967755098 4.998168581512297E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAG 2600 0.0 20.567049 1 GTACTAG 1710 0.0 20.122551 1 GTATCAA 21795 0.0 19.137405 1 GTACATG 33960 0.0 18.662748 1 TCTAACG 935 0.0 18.400837 2 TACATGG 33775 0.0 18.117905 2 TATAAGG 2745 0.0 17.617264 2 GTATATA 2045 0.0 16.826195 1 CATGGGG 13890 0.0 16.77055 4 ACATGGG 33810 0.0 16.723833 3 TAGTACT 2440 0.0 16.197235 4 CTAGTAC 2550 0.0 16.046856 3 AACGCAG 24250 0.0 15.9136 6 GTATTAT 1940 0.0 15.819386 1 TAGGACT 2745 0.0 15.752606 4 CAACGCA 24355 0.0 15.692621 5 GTGATCG 1660 0.0 15.684676 8 ATAAGGT 2355 0.0 15.598528 3 GTACATA 1825 0.0 15.542269 1 ATCAACG 24535 0.0 15.5219145 3 >>END_MODULE