FastQCFastQC Report
Fri 27 May 2016
ERR765262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6702455
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125840.18775209979030072No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97770.14587192304909172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93680.13976968140778268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.1129138502235375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71290.10636401139582437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA268500.028.6273211
TATCACG6700.024.2933712
CAACGCA329450.023.0223315
ATCAACG331450.022.869383
TCAACGC343200.022.1541614
AACGCAG346200.022.095526
TATCAAC358600.021.3329122
AGTACTT184450.020.6870112-13
ACGCAGA372950.020.2239347
CGCAGAG377050.020.0049178
GTACTAG16150.019.0145631
ATCACGC9600.018.8920123
GCAGAGT406650.018.4687129
GGTATCA192800.018.1478731
TCTAACG9500.018.1122632
TACTTTT217100.017.8325114-15
TCACGCA9700.017.7384154
TAGTACT25600.017.6204364
TAACGCA4500.017.5679554
AGAGTAC387350.017.52859510-11
Produced by FastQC (version 0.11.5)