##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6702455 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33139528724922 33.0 33.0 33.0 33.0 33.0 2 32.116711264752986 33.0 33.0 33.0 27.0 33.0 3 32.19069281330498 33.0 33.0 33.0 33.0 33.0 4 36.148411141887564 37.0 37.0 37.0 33.0 37.0 5 36.09172027264636 37.0 37.0 37.0 33.0 37.0 6 36.1041942094352 37.0 37.0 37.0 33.0 37.0 7 36.02807687630875 37.0 37.0 37.0 33.0 37.0 8 35.983049196152756 37.0 37.0 37.0 33.0 37.0 9 35.7483889112273 37.0 37.0 37.0 33.0 37.0 10-11 35.90949845989268 37.0 37.0 37.0 33.0 37.0 12-13 35.843510400293624 37.0 37.0 37.0 33.0 37.0 14-15 37.26645587325838 37.0 37.0 40.0 33.0 40.0 16-17 37.39946907513739 37.0 37.0 40.0 33.0 40.0 18-19 37.445600753753666 38.5 37.0 40.0 33.0 40.0 20-21 37.468320936134596 38.5 37.0 40.0 33.0 40.0 22-23 37.39264083085973 37.0 37.0 40.0 33.0 40.0 24-25 37.34546162562822 37.0 37.0 40.0 33.0 40.0 26-27 37.236030902109746 37.0 37.0 40.0 33.0 40.0 28-29 37.18370283724397 37.0 37.0 40.0 33.0 40.0 30-31 37.11830650112533 37.0 37.0 40.0 33.0 40.0 32-33 36.98088178137712 37.0 37.0 40.0 33.0 40.0 34-35 36.91235815831662 37.0 37.0 40.0 33.0 40.0 36-37 36.789448716925364 37.0 37.0 40.0 33.0 40.0 38-39 36.66219034667148 37.0 37.0 40.0 33.0 40.0 40-41 36.562807060994814 37.0 37.0 40.0 33.0 40.0 42-43 36.420717259571305 37.0 37.0 40.0 33.0 40.0 44-45 36.27243099729875 37.0 37.0 40.0 33.0 40.0 46-47 36.09338242479808 37.0 37.0 40.0 33.0 40.0 48-49 35.9125113111539 37.0 37.0 40.0 33.0 40.0 50-51 35.64710139792061 37.0 37.0 40.0 27.0 40.0 52-53 35.50245589116227 37.0 33.0 40.0 27.0 40.0 54-55 35.38420474288898 37.0 33.0 40.0 27.0 40.0 56-57 35.2082148257616 37.0 33.0 40.0 27.0 40.0 58-59 35.01499331215204 37.0 33.0 40.0 27.0 40.0 60-61 34.83946434552712 37.0 33.0 37.0 27.0 40.0 62-63 34.52014500656849 37.0 33.0 37.0 27.0 40.0 64-65 34.37077779709077 37.0 33.0 37.0 27.0 40.0 66-67 34.17200973374682 37.0 33.0 37.0 27.0 40.0 68-69 33.92510923236337 37.0 33.0 37.0 27.0 40.0 70-71 33.7141362381396 37.0 33.0 37.0 27.0 38.5 72-73 33.50278427829802 37.0 33.0 37.0 27.0 37.0 74-75 33.300453266750765 37.0 33.0 37.0 27.0 37.0 76-77 33.13340820639601 37.0 33.0 37.0 27.0 37.0 78-79 32.93969963244811 33.0 33.0 37.0 27.0 37.0 80-81 32.79627822939505 33.0 33.0 37.0 27.0 37.0 82-83 32.66096325898495 33.0 33.0 37.0 27.0 37.0 84-85 32.511932642591404 33.0 33.0 37.0 27.0 37.0 86-87 32.41366327711264 33.0 33.0 37.0 27.0 37.0 88-89 32.312967412686845 33.0 33.0 37.0 27.0 37.0 90-91 32.25801486171858 33.0 33.0 37.0 24.5 37.0 92-93 32.168213214411736 33.0 33.0 37.0 22.0 37.0 94-95 32.10681093718645 33.0 33.0 37.0 22.0 37.0 96-97 32.05668564727402 33.0 33.0 37.0 22.0 37.0 98-99 31.244319879805236 33.0 30.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1564885686391051 1101 2 0.35677140937838203 1101 3 0.24400974757024585 1101 4 0.1936626108584676 1101 5 0.05824210831893595 1101 6 0.11547940306792981 1101 7 0.17768350167509794 1101 8 0.1524912149372568 1101 9 0.4008435403062123 1101 10-11 0.30994811902331776 1101 12-13 0.3171896074414491 1101 14-15 0.534668288990396 1101 16-17 0.38714269570723303 1101 18-19 0.30093212816989734 1101 20-21 0.3352312760531788 1101 22-23 0.18765482407896883 1101 24-25 0.3200780250974802 1101 26-27 0.2162163955009504 1101 28-29 0.20632736417410769 1101 30-31 0.2487674225589913 1101 32-33 0.3565216445873318 1101 34-35 0.37672965603022845 1101 36-37 0.43217079503862266 1101 38-39 0.27619311947800895 1101 40-41 0.23195683922261168 1101 42-43 0.36444576087917113 1101 44-45 0.3889253979222005 1101 46-47 0.45797934505942806 1101 48-49 0.38649493097398135 1101 50-51 0.42225359756081815 1101 52-53 0.43318813092478337 1101 54-55 0.4271402381571008 1101 56-57 0.5298466760707043 1101 58-59 0.46082064340809836 1101 60-61 0.3822677709253739 1101 62-63 0.36807577062906205 1101 64-65 0.2909504915243204 1101 66-67 0.28483651261291953 1101 68-69 0.18580377043456053 1101 70-71 0.23485150652589937 1101 72-73 0.313678001609226 1101 74-75 0.17502798230637495 1101 76-77 0.19767055080079388 1101 78-79 0.4681396971987226 1101 80-81 0.4459592551857341 1101 82-83 0.3633544237828161 1101 84-85 0.351012551657675 1101 86-87 0.15403457181081848 1101 88-89 0.2805147331130726 1101 90-91 0.24212437921737973 1101 92-93 0.3778815233991182 1101 94-95 0.3708484042863134 1101 96-97 0.2955359602036651 1101 98-99 0.40026924844767464 1102 1 0.10233188979393049 1102 2 0.3254143547072488 1102 3 0.18222519785171443 1102 4 0.18860634632464723 1102 5 0.039224588854033016 1102 6 0.1806243655062758 1102 7 0.17291724112942575 1102 8 0.14109962113456476 1102 9 0.40738199056860935 1102 10-11 0.26691439008616413 1102 12-13 0.2719079264439799 1102 14-15 0.47519466870584637 1102 16-17 0.30900373689777183 1102 18-19 0.25624389478923604 1102 20-21 0.3662208719658224 1102 22-23 0.17832429780628445 1102 24-25 0.33524848934678175 1102 26-27 0.2535621502638108 1102 28-29 0.27516583579659226 1102 30-31 0.25911554554659233 1102 32-33 0.3113446001262332 1102 34-35 0.35407898942313665 1102 36-37 0.32435251219468597 1102 38-39 0.20898555381666029 1102 40-41 0.22407823156564888 1102 42-43 0.24575051822338168 1102 44-45 0.3360952413792404 1102 46-47 0.4271337087310769 1102 48-49 0.47071557841283607 1102 50-51 0.37599617911659067 1102 52-53 0.44749926325638256 1102 54-55 0.30469458656489223 1102 56-57 0.45418668873784895 1102 58-59 0.3496191404765554 1102 60-61 0.2826849861369283 1102 62-63 0.3750529078832514 1102 64-65 0.2959170808944336 1102 66-67 0.37157597578118384 1102 68-69 0.29716916903763746 1102 70-71 0.36682590512758395 1102 72-73 0.37355774754045257 1102 74-75 0.2788730411210949 1102 76-77 0.27134463114173 1102 78-79 0.4897667406592987 1102 80-81 0.40299545612325005 1102 82-83 0.41367146992853066 1102 84-85 0.41212579412452044 1102 86-87 0.2647761905336097 1102 88-89 0.273459354627569 1102 90-91 0.22731338812300095 1102 92-93 0.24374031782426897 1102 94-95 0.28531187736305696 1102 96-97 0.2385534433703711 1102 98-99 0.16880538865551387 1103 1 0.08434991776989165 1103 2 0.2936203814860079 1103 3 0.22623690649686523 1103 4 0.22075524459026497 1103 5 0.10580372896846768 1103 6 0.12961882970497385 1103 7 0.14864933262823854 1103 8 0.14064665060141834 1103 9 0.41981952676554357 1103 10-11 0.2541125818040939 1103 12-13 0.267511198470757 1103 14-15 0.45233463829749354 1103 16-17 0.43380477829123976 1103 18-19 0.2774210734782656 1103 20-21 0.352894804386338 1103 22-23 0.19448792820816863 1103 24-25 0.28403267794136866 1103 26-27 0.16521107091264753 1103 28-29 0.09992939527534617 1103 30-31 0.13979390445186368 1103 32-33 0.17984545713360944 1103 34-35 0.24771699690967353 1103 36-37 0.24573368512641736 1103 38-39 0.1028978692928817 1103 40-41 0.02915984355986012 1103 42-43 0.10768459158546762 1103 44-45 0.16062538734355059 1103 46-47 0.28629310788603846 1103 48-49 0.21511406422973778 1103 50-51 0.1890768282812303 1103 52-53 0.14479855442350242 1103 54-55 0.12553792495846494 1103 56-57 0.2432050455477679 1103 58-59 0.24094688199197378 1103 60-61 0.1450090523527905 1103 62-63 0.16895097779433854 1103 64-65 -0.011653267423461955 1103 66-67 0.02306529359955789 1103 68-69 0.05962901109889884 1103 70-71 0.003863460402982355 1103 72-73 0.11175233427257325 1103 74-75 0.1431382823874756 1103 76-77 0.10092524948551329 1103 78-79 0.29003821266119445 1103 80-81 0.08804712852400343 1103 82-83 0.24884192334204158 1103 84-85 0.2311201012891928 1103 86-87 0.16000692626619184 1103 88-89 0.17530051540440894 1103 90-91 0.0431367562405498 1103 92-93 0.09403406756499066 1103 94-95 0.26121054681602374 1103 96-97 0.20453733542846209 1103 98-99 0.11820182717048056 1104 1 0.12850153769561956 1104 2 0.31719830439546826 1104 3 0.21281777472582775 1104 4 0.17925825393844974 1104 5 0.06795765392684672 1104 6 0.1396380383104301 1104 7 0.13965458298039124 1104 8 0.01567674408329367 1104 9 0.3399283755297873 1104 10-11 0.23258869910463886 1104 12-13 0.22540721588656254 1104 14-15 0.41535487136190596 1104 16-17 0.2335390592491109 1104 18-19 0.1810879962596772 1104 20-21 0.2994462194202754 1104 22-23 0.2121726509639572 1104 24-25 0.30321951364376787 1104 26-27 0.12049727913392871 1104 28-29 0.1071844855557913 1104 30-31 0.13278084260015532 1104 32-33 0.14042811816575806 1104 34-35 0.2562174451104724 1104 36-37 0.13803679692050963 1104 38-39 -0.00913760259852836 1104 40-41 -0.00945457569996222 1104 42-43 0.09942937324765211 1104 44-45 0.1398032590300673 1104 46-47 0.29990048399054103 1104 48-49 0.40124609137789946 1104 50-51 0.2695551225570654 1104 52-53 0.29091265353990536 1104 54-55 0.25563460827259377 1104 56-57 0.2688158792012416 1104 58-59 0.23413358963699693 1104 60-61 0.1468626827792221 1104 62-63 0.24287017359405638 1104 64-65 0.0905678876540108 1104 66-67 0.159537313064277 1104 68-69 0.08713573411080944 1104 70-71 0.1802560842984846 1104 72-73 0.17582968092387574 1104 74-75 0.12446431645074085 1104 76-77 0.07983740212989687 1104 78-79 0.28349896307737765 1104 80-81 0.22112114568369634 1104 82-83 0.19164702002535705 1104 84-85 0.22287512754094507 1104 86-87 0.19492176811533568 1104 88-89 0.08770991481534196 1104 90-91 0.16917914322991834 1104 92-93 0.1595238870040987 1104 94-95 0.2312105468160226 1104 96-97 0.1808537651889921 1104 98-99 0.05570022645336081 1105 1 0.07671833419215801 1105 2 0.2566041648741333 1105 3 0.20177008541308794 1105 4 0.13203832127729243 1105 5 -0.04599034589075046 1105 6 0.05458275564388515 1105 7 0.09096199982839437 1105 8 -0.06068814948331891 1105 9 0.2758031475722973 1105 10-11 0.18406236457145297 1105 12-13 0.16844838505294035 1105 14-15 0.3102418993503875 1105 16-17 0.2996519222433207 1105 18-19 0.11550944032377686 1105 20-21 0.26288550698419044 1105 22-23 0.08013396393243255 1105 24-25 0.23181290734298443 1105 26-27 0.05775938256493873 1105 28-29 0.031556074666994505 1105 30-31 0.07824353976988618 1105 32-33 0.0952389709111543 1105 34-35 0.1237308536283237 1105 36-37 0.03663705337604739 1105 38-39 0.0026968736755392797 1105 40-41 -0.06386352944755203 1105 42-43 0.04002785364340866 1105 44-45 0.07439441059550234 1105 46-47 0.1888810232339324 1105 48-49 0.2163187429133444 1105 50-51 0.1064546934259667 1105 52-53 0.04901464828995472 1105 54-55 0.05078596711982897 1105 56-57 0.1655431345621281 1105 58-59 0.06888706049364401 1105 60-61 0.07152178050336744 1105 62-63 0.1032664011358122 1105 64-65 -0.11137416236704212 1105 66-67 -0.015055899198721079 1105 68-69 -0.016681546062528696 1105 70-71 0.03644948248729918 1105 72-73 0.021964583899027446 1105 74-75 0.023743310304318754 1105 76-77 -0.00429297905321846 1105 78-79 0.18011327457935522 1105 80-81 0.10575611068721003 1105 82-83 -0.025962665816244623 1105 84-85 -0.0314644920497571 1105 86-87 -0.07669944263023609 1105 88-89 -0.058412920502988186 1105 90-91 -0.08048989218322333 1105 92-93 -0.12459566005139067 1105 94-95 0.08217885611757225 1105 96-97 -0.03520716548544556 1105 98-99 -0.052685339247389606 1106 1 0.09552623947092798 1106 2 0.22640840356819325 1106 3 0.18051778419263798 1106 4 0.11995486525299981 1106 5 -0.016701002297921264 1106 6 0.08677268561162066 1106 7 0.05611925802864448 1106 8 -0.009903681016815824 1106 9 0.37469069002778355 1106 10-11 0.18417971353973428 1106 12-13 0.14546159761547983 1106 14-15 0.3462038458922265 1106 16-17 0.2714469891290108 1106 18-19 0.11959120958547942 1106 20-21 0.2208117653784143 1106 22-23 0.024002856717864063 1106 24-25 0.14736292753396185 1106 26-27 -0.02424335776169073 1106 28-29 0.1057275152152144 1106 30-31 0.027806006276613005 1106 32-33 0.029720756045158225 1106 34-35 0.1090105611334522 1106 36-37 0.026519063736877513 1106 38-39 -0.0528227066421465 1106 40-41 -0.10544392594166396 1106 42-43 -0.03875677159513202 1106 44-45 0.044986572335950825 1106 46-47 0.17276364121064347 1106 48-49 0.2406230927631583 1106 50-51 0.061448847235091364 1106 52-53 -0.006582218360073 1106 54-55 -0.006445226856783393 1106 56-57 0.1434562357370197 1106 58-59 0.13109292312368126 1106 60-61 0.06847845574648659 1106 62-63 0.14615610089085607 1106 64-65 -0.018492196306546305 1106 66-67 0.030448279803067635 1106 68-69 -0.027074586149694824 1106 70-71 -0.007955672629321953 1106 72-73 0.12115415823091524 1106 74-75 0.016673122388233708 1106 76-77 0.018150820941215784 1106 78-79 0.07412311956147732 1106 80-81 0.12758428411683553 1106 82-83 0.07616646244122194 1106 84-85 0.024309046821691993 1106 86-87 0.03495225256450851 1106 88-89 -0.025436480056832522 1106 90-91 -0.0896163833465593 1106 92-93 -0.12299755796113487 1106 94-95 0.09014752007804105 1106 96-97 0.0849517409561571 1106 98-99 -0.043116801735223476 1107 1 0.12030554225642476 1107 2 0.23251337079280887 1107 3 0.17287491744054506 1107 4 0.16185984099701756 1107 5 0.03298862686388304 1107 6 0.11734560955060402 1107 7 0.07794329544962153 1107 8 0.018631315703906637 1107 9 0.4230333413125891 1107 10-11 0.2170702498448165 1107 12-13 0.14818776974757242 1107 14-15 0.28883379759188443 1107 16-17 0.18266814848190904 1107 18-19 0.13784586717865466 1107 20-21 0.23774178621488318 1107 22-23 0.06748750036874895 1107 24-25 0.16501704288305774 1107 26-27 0.03623603997378666 1107 28-29 0.023736423216767832 1107 30-31 0.05281230340387566 1107 32-33 -0.01764931210587406 1107 34-35 0.0867290383954682 1107 36-37 0.037688094112581894 1107 38-39 0.010073142542516678 1107 40-41 -0.027000860631787305 1107 42-43 -0.03785815045429075 1107 44-45 -0.05432617255245731 1107 46-47 0.06296864937682756 1107 48-49 0.14250222182971584 1107 50-51 -0.02738637207367134 1107 52-53 -0.011966492094458658 1107 54-55 0.011531999278098226 1107 56-57 0.13276917166545132 1107 58-59 0.027820076935618943 1107 60-61 0.03430563838924883 1107 62-63 0.09225342451114926 1107 64-65 -0.1116132274113042 1107 66-67 -0.16538693289486872 1107 68-69 -0.17268507549644596 1107 70-71 -0.10494789584332409 1107 72-73 0.054335058985181206 1107 74-75 -0.022242846388934367 1107 76-77 0.0615723339676606 1107 78-79 0.08843680717505009 1107 80-81 -0.034492139032565206 1107 82-83 -0.09735264818131384 1107 84-85 -0.06313910625378583 1107 86-87 -0.0037632698783340857 1107 88-89 -0.07837027481708247 1107 90-91 -0.06060969168247965 1107 92-93 -0.1095061027954145 1107 94-95 0.09877677893434367 1107 96-97 -0.03353769827288744 1107 98-99 -0.057479947590060476 1108 1 0.11186143317102903 1108 2 0.2536184954772125 1108 3 0.1647443940211204 1108 4 0.13430198813951932 1108 5 -0.03230997101329791 1108 6 0.09302030203993183 1108 7 0.06910646787795116 1108 8 0.01652644250253843 1108 9 0.34711990625547173 1108 10-11 0.24160537579437857 1108 12-13 0.15991894525188854 1108 14-15 0.2912189392850877 1108 16-17 0.26935521203859736 1108 18-19 0.06408398199505427 1108 20-21 0.24331687512692213 1108 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2215 78-79 -0.2679150470364604 2215 80-81 -0.1486163511767984 2215 82-83 -0.16029373330805186 2215 84-85 -0.05851365389778351 2215 86-87 -0.21342221173409115 2215 88-89 -0.17594811359455775 2215 90-91 -0.2811187005066422 2215 92-93 -0.1315107079076725 2215 94-95 -0.16595074720394365 2215 96-97 -0.20149069402954467 2215 98-99 -0.06345177945915736 2216 1 -0.19913456970536458 2216 2 -0.003388923392897425 2216 3 -0.08198400386617521 2216 4 -0.30786020914148793 2216 5 -0.2778663518174582 2216 6 -0.2239960452653662 2216 7 -0.24496192078559886 2216 8 -0.21852933068567637 2216 9 0.07646704273305005 2216 10-11 -0.2048005845021379 2216 12-13 -0.11489317123515264 2216 14-15 -0.38464810085989853 2216 16-17 -0.5736706415399837 2216 18-19 -0.5344735392272071 2216 20-21 -0.8159203392209804 2216 22-23 -0.6742081853803441 2216 24-25 -0.5728917037294252 2216 26-27 -0.6707440401235942 2216 28-29 -0.6847345047971984 2216 30-31 -0.42030305240920995 2216 32-33 -0.5564635349849141 2216 34-35 -0.53060230289217 2216 36-37 -0.43742889640157756 2216 38-39 -0.5432776868051477 2216 40-41 -0.5415826557496359 2216 42-43 -0.5801030614281615 2216 44-45 -0.5230734825245307 2216 46-47 -0.8179821395774383 2216 48-49 -0.9377083316591097 2216 50-51 -0.6096839805177794 2216 52-53 -0.5304560604824147 2216 54-55 -0.47124519308708557 2216 56-57 -0.6113411653987271 2216 58-59 -0.45377660185371127 2216 60-61 -0.48886636839528563 2216 62-63 -0.5428434690759403 2216 64-65 -0.6352831901903286 2216 66-67 -0.5578762423300674 2216 68-69 -0.47302103995816935 2216 70-71 -0.4749628114681883 2216 72-73 -0.41220568317736905 2216 74-75 -0.42780239399549913 2216 76-77 -0.421484277500241 2216 78-79 -0.3138190659224662 2216 80-81 -0.2741914976683333 2216 82-83 -0.25015071091628016 2216 84-85 -0.34276712389942077 2216 86-87 -0.4784565252376183 2216 88-89 -0.30748945838514885 2216 90-91 -0.3108089868833659 2216 92-93 -0.3319835840848242 2216 94-95 -0.3920403617247743 2216 96-97 -0.4160572187132381 2216 98-99 -0.30672170183004965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 22.0 10 61.0 11 120.0 12 224.0 13 363.0 14 877.0 15 2055.0 16 4491.0 17 8367.0 18 13526.0 19 19133.0 20 24954.0 21 32099.0 22 39461.0 23 41346.0 24 41050.0 25 45454.0 26 56277.0 27 73855.0 28 98468.0 29 131568.0 30 175287.0 31 233244.0 32 310189.0 33 410498.0 34 547040.0 35 764780.0 36 1223003.0 37 1775751.0 38 628164.0 39 722.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.053741604343784 18.304527149506683 12.622463165698116 26.019268080451408 2 16.192393626529526 20.796173506462193 36.866586908261624 26.14484595874666 3 18.406613696026305 24.83715295365653 29.409955009022816 27.346278341294344 4 13.65402677078772 16.9055965314202 36.085792444708694 33.35458425308339 5 14.691470276489996 36.68556365094749 33.43290605737967 15.190060015182837 6 32.622583814438144 36.24618143650349 17.12235292888949 14.008881820168877 7 28.23016342519271 31.141335525564884 22.12449020545457 18.504010843787835 8 27.161122305185188 32.66816412792029 20.241956119063836 19.928757447830684 9 27.130485901602164 13.931019459340096 19.36286402334763 39.57563061571011 10-11 25.715577351880764 24.64760300516751 27.48438445315933 22.152435189792396 12-13 26.777516596530678 22.309437362876736 27.19684802061334 23.716198019979245 14-15 24.2666161167649 23.42843220106328 24.84012176140446 27.464829920767357 16-17 24.115973318989667 25.9535865010381 25.479283125029372 24.451157054942858 18-19 24.397716955951214 24.963606618768793 27.33621486455336 23.302461560726627 20-21 25.257506391314827 24.507557305494778 26.637620095920077 23.59731620727032 22-23 24.640150512013882 24.2052949255161 26.63483007345816 24.519724489011864 24-25 24.398686749855088 24.652675773280087 26.596500834395755 24.352136642469066 26-27 24.629199300853195 24.699039381838446 26.417603698943147 24.25415761836521 28-29 24.327764975669364 24.46905648751092 26.932758220681823 24.27042031613789 30-31 24.651847718485243 24.45368152415794 26.681812858124378 24.212657899232447 32-33 24.397172379374425 24.468862528730146 26.39391088787616 24.740054204019273 34-35 24.769692597712332 24.425527661133124 26.65621029906206 24.148569442092487 36-37 24.811132637220243 24.52726650160277 26.20535311315033 24.456247748026655 38-39 24.780173831827295 24.23000975015871 26.419655186047503 24.570161231966495 40-41 24.678367851779683 24.37645608959702 26.5135909155675 24.43158514305579 42-43 25.071373101348687 24.447892600547114 26.129970287006778 24.350764011097425 44-45 24.813071388340788 24.554940935556907 25.85007665136126 24.781911024741046 46-47 24.683321260642554 24.656174491287146 25.995213694086715 24.665290553983578 48-49 25.34821196113961 24.781449483808547 25.46083487319199 24.409503681859857 50-51 24.647118108215572 25.14520425724604 25.355000518466742 24.852677116071646 52-53 24.754313721101987 24.925184074480228 25.25414587203187 25.06635633238592 54-55 24.83474338880306 24.839323800010593 25.362900608806772 24.963032202379576 56-57 24.88247118680938 24.60306376865955 25.221976489313754 25.292488555217318 58-59 24.594316560126103 24.64463394383103 25.676718456147785 25.084331039895087 60-61 24.704380708262867 24.7446271552737 25.520641317248682 25.030350819214753 62-63 24.529206089410522 24.815586229224962 25.383616898584176 25.27159078278034 64-65 24.496732913536906 24.48968325785104 25.507422280343544 25.50616154826851 66-67 24.843546133469005 24.67389933986875 25.462028465689063 25.020526060973182 68-69 24.76269325595349 24.36804800585944 25.52265821450496 25.34660052368211 70-71 24.309472140390557 24.48594505437941 25.51586025696593 25.688722548264103 72-73 24.809237809131133 24.670833299141883 25.36319900693104 25.156729884795944 74-75 24.97207366554494 24.480186737546166 25.263772751924478 25.283966844984413 76-77 24.690632014687157 24.717278967184413 25.32043855572324 25.271650462405194 78-79 24.720009984443696 24.77399789464308 25.31121378205341 25.194778338859813 80-81 24.451873787048747 24.6557409824313 25.451008052709454 25.44137717781049 82-83 24.5603066339125 24.83519844594257 25.08874733213427 25.51574758801066 84-85 24.523223207018923 24.90403143325841 24.896481960714397 25.676263399008274 86-87 24.351733805001302 24.669020530536944 25.102085728289115 25.87715993617264 88-89 24.349406299632 24.443707566854233 25.286339110072355 25.920547023441408 90-91 24.616450475063022 24.643015481035118 25.020251993811492 25.720282050090365 92-93 24.231486915569995 24.741679236216957 25.027048249840412 25.99978559837264 94-95 23.883006480119686 24.758428762137868 25.408745867632188 25.949818890110254 96-97 24.381935491956618 24.641647055014555 25.08486653843965 25.891550914589185 98-99 24.02623367109514 24.657673941861603 25.073626007186917 26.24246637985634 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 75.0 1 103.5 2 437.5 3 744.0 4 531.5 5 437.5 6 950.5 7 2431.0 8 4286.5 9 4220.5 10 2547.0 11 1550.5 12 1407.0 13 1528.0 14 1734.0 15 2042.0 16 2418.5 17 2808.5 18 3345.0 19 4015.0 20 4944.0 21 6154.5 22 7701.0 23 9700.5 24 12269.5 25 15683.5 26 19639.5 27 23381.0 28 27963.5 29 33794.5 30 39163.0 31 45196.5 32 52102.5 33 60278.0 34 68787.0 35 76837.5 36 85084.5 37 94252.0 38 106337.0 39 115596.0 40 124830.0 41 136345.0 42 151711.0 43 173136.5 44 193929.0 45 214631.0 46 245533.5 47 271826.0 48 285468.0 49 299295.0 50 316494.0 51 316546.5 52 308916.5 53 312451.0 54 306899.5 55 295820.0 56 272002.0 57 251952.0 58 239123.0 59 212910.0 60 178355.0 61 138413.5 62 113932.0 63 92917.0 64 67992.0 65 50452.0 66 36138.5 67 27482.5 68 21438.5 69 18533.0 70 16820.0 71 14899.0 72 15084.5 73 12380.5 74 7667.5 75 5766.5 76 4489.0 77 2938.0 78 1535.0 79 984.0 80 671.0 81 472.0 82 309.5 83 209.5 84 141.5 85 78.5 86 41.0 87 22.5 88 14.0 89 7.5 90 3.5 91 3.5 92 1.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05377134199334423 2 0.008191028511194779 3 0.0 4 0.0 5 0.002252905838233901 6 0.0 7 0.0 8 0.0 9 0.004102974208704124 10-11 0.0 12-13 0.0 14-15 0.0024916243376494136 16-17 0.004625170926175558 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 4.475971864040863E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.459953106734771E-5 54-55 0.0 56-57 8.355147479542944E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0015889700117345063 70-71 1.4919906213469543E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.25217327083882E-4 80-81 7.83295076207151E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 4.25217327083882E-4 92-93 0.001201052450184298 94-95 4.8489695193776013E-4 96-97 2.9093817116265607E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6702455.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.05820539852595 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8565516944183 31.80130572885113 2 14.419265364904724 12.417353795558377 3 4.699754592915329 6.070889957532418 4 2.1539283631565156 3.7097715949801624 5 1.1635680389529774 2.505057580819867 6 0.7007221494129177 1.8103102942030795 7 0.5094315963303281 1.535464721790316 8 0.36339827262739816 1.2517821971408032 9 0.27113081551823126 1.0506965710008465 >10 1.5749326012830092 12.631059212785864 >50 0.14522351873480682 4.358912204702921 >100 0.12050881316226186 10.570664871458092 >500 0.014599050513985029 4.380282407703643 >1k 0.006810499867742298 5.208278240771124 >5k 1.3970256136335317E-4 0.5089279745352482 >10k+ 3.492564034083829E-5 0.1892426461660529 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12584 0.18775209979030072 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9777 0.14587192304909172 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9368 0.13976968140778268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7568 0.1129138502235375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7129 0.10636401139582437 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9839812426939084E-5 2 0.0 0.0 0.0 0.0 2.9839812426939084E-5 3 0.0 0.0 0.0 0.0 2.9839812426939084E-5 4 0.0 0.0 0.0 0.0 2.9839812426939084E-5 5 0.0 1.4919906213469542E-5 0.0 1.4919906213469542E-5 2.9839812426939084E-5 6 0.0 1.4919906213469542E-5 0.0 1.4919906213469542E-5 2.9839812426939084E-5 7 0.0 1.4919906213469542E-5 0.0 1.4919906213469542E-5 2.9839812426939084E-5 8 0.0 1.4919906213469542E-5 0.0 1.4919906213469542E-5 2.9839812426939084E-5 9 0.0 2.9839812426939084E-5 0.0 1.4919906213469542E-5 5.967962485387817E-5 10-11 7.459953106734771E-6 2.9839812426939084E-5 0.0 1.4919906213469542E-5 5.967962485387817E-5 12-13 1.4919906213469542E-5 2.9839812426939084E-5 0.0 1.4919906213469542E-5 5.967962485387817E-5 14-15 2.2379859320204312E-5 5.2219671747143393E-5 0.0 1.4919906213469542E-5 8.205948417408248E-5 16-17 2.9839812426939084E-5 5.967962485387817E-5 0.0 1.4919906213469542E-5 8.951943728081725E-5 18-19 2.9839812426939084E-5 6.713957796061294E-5 0.0 6.713957796061293E-5 8.951943728081725E-5 20-21 2.9839812426939084E-5 8.951943728081725E-5 0.0 1.8649882766836928E-4 9.697939038755203E-5 22-23 3.7299765533673856E-5 8.951943728081725E-5 0.0 6.564758733926598E-4 1.044393434942868E-4 24-25 4.4759718640408625E-5 8.951943728081725E-5 0.0 0.0016859494021220583 1.044393434942868E-4 26-27 4.4759718640408625E-5 8.951943728081725E-5 0.0 0.003215239789002686 1.044393434942868E-4 28-29 4.4759718640408625E-5 8.951943728081725E-5 0.0 0.009712858944968672 1.044393434942868E-4 30-31 4.4759718640408625E-5 8.951943728081725E-5 0.0 0.027930064431614984 1.044393434942868E-4 32-33 4.4759718640408625E-5 8.951943728081725E-5 0.0 0.060820997679208594 1.1189929660102157E-4 34-35 4.4759718640408625E-5 1.1935924970775635E-4 0.0 0.10446172335360701 1.1935924970775634E-4 36-37 5.2219671747143393E-5 1.342791559212259E-4 0.0 0.16734912804338112 1.1935924970775634E-4 38-39 5.967962485387817E-5 1.342791559212259E-4 0.0 0.26304540649657476 1.1935924970775634E-4 40-41 5.967962485387817E-5 1.342791559212259E-4 0.0 0.37896561782212634 1.1935924970775634E-4 42-43 5.967962485387817E-5 1.342791559212259E-4 0.0 0.48699319876075264 1.1935924970775634E-4 44-45 5.967962485387817E-5 1.566590152414302E-4 0.0 0.6126561088436998 1.4173910902796064E-4 46-47 5.967962485387817E-5 1.790388745616345E-4 0.0 0.7533433644836109 1.4919906213469543E-4 48-49 5.967962485387817E-5 1.790388745616345E-4 0.0 0.8983350130661079 1.4919906213469543E-4 50-51 5.967962485387817E-5 1.790388745616345E-4 0.0 1.0561279411797617 1.4919906213469543E-4 52-53 7.459953106734771E-5 1.790388745616345E-4 0.0 1.2159798163508744 2.0141873388183882E-4 54-55 7.459953106734771E-5 2.3871849941551268E-4 0.0 1.3790320114047763 2.2379859320204314E-4 56-57 7.459953106734771E-5 2.536384056289822E-4 0.0 1.5603237918046449 2.3125854630877792E-4 58-59 7.459953106734771E-5 2.61098358735717E-4 0.0 1.7579827093206892 2.4617845252224743E-4 60-61 7.459953106734771E-5 2.685583118424518E-4 0.0 1.9609083537300882 2.536384056289822E-4 62-63 7.459953106734771E-5 2.9093817116265607E-4 0.0 2.1689216861582805 3.058580773761256E-4 64-65 7.459953106734771E-5 3.282379366963299E-4 0.0 2.385827282689701 3.2077798358959514E-4 66-67 7.459953106734771E-5 3.282379366963299E-4 0.0 2.628365278095862 3.431578429097995E-4 68-69 7.459953106734771E-5 3.431578429097995E-4 0.0 2.8890757192700285 3.431578429097995E-4 70-71 7.459953106734771E-5 3.5061779601653427E-4 0.0 3.179566293246281 3.58077749123269E-4 72-73 7.459953106734771E-5 3.58077749123269E-4 0.0 3.469281032099432 3.879175615502081E-4 74-75 7.459953106734771E-5 3.58077749123269E-4 0.0 3.7581154964859893 3.879175615502081E-4 76-77 7.459953106734771E-5 3.655377022300038E-4 0.0 4.070956089970018 3.879175615502081E-4 78-79 7.459953106734771E-5 3.879175615502081E-4 0.0 4.400708397147016 3.879175615502081E-4 80-81 7.459953106734771E-5 3.879175615502081E-4 0.0 4.757764132694662 3.9537751465694286E-4 82-83 7.459953106734771E-5 3.879175615502081E-4 0.0 5.137856203435906 4.0283746776367764E-4 84-85 7.459953106734771E-5 3.879175615502081E-4 0.0 5.522110928010706 4.0283746776367764E-4 86-87 7.459953106734771E-5 3.879175615502081E-4 0.0 5.9205917831600505 4.0283746776367764E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 26850 0.0 28.627321 1 TATCACG 670 0.0 24.293371 2 CAACGCA 32945 0.0 23.022331 5 ATCAACG 33145 0.0 22.86938 3 TCAACGC 34320 0.0 22.154161 4 AACGCAG 34620 0.0 22.09552 6 TATCAAC 35860 0.0 21.332912 2 AGTACTT 18445 0.0 20.68701 12-13 ACGCAGA 37295 0.0 20.223934 7 CGCAGAG 37705 0.0 20.004917 8 GTACTAG 1615 0.0 19.014563 1 ATCACGC 960 0.0 18.892012 3 GCAGAGT 40665 0.0 18.468712 9 GGTATCA 19280 0.0 18.147873 1 TCTAACG 950 0.0 18.112263 2 TACTTTT 21710 0.0 17.83251 14-15 TCACGCA 970 0.0 17.738415 4 TAGTACT 2560 0.0 17.620436 4 TAACGCA 450 0.0 17.567955 4 AGAGTAC 38735 0.0 17.528595 10-11 >>END_MODULE