##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765260_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5335892 Sequences flagged as poor quality 0 Sequence length 99 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.990488750521937 33.0 27.0 33.0 22.0 33.0 2 29.845250053786696 33.0 27.0 33.0 22.0 33.0 3 29.914267755044516 33.0 27.0 33.0 22.0 33.0 4 32.9790411799939 37.0 33.0 37.0 22.0 37.0 5 33.5003920619083 37.0 33.0 37.0 27.0 37.0 6 33.27000509005804 37.0 33.0 37.0 22.0 37.0 7 33.21306596910132 37.0 33.0 37.0 22.0 37.0 8 33.01872676583409 37.0 33.0 37.0 22.0 37.0 9 32.6866150964075 37.0 33.0 37.0 15.0 37.0 10-11 32.934710635072825 37.0 33.0 37.0 15.0 37.0 12-13 32.800660976646455 37.0 33.0 37.0 15.0 37.0 14-15 33.880489616356556 37.0 33.0 40.0 15.0 40.0 16-17 34.042640574434415 37.0 33.0 40.0 18.5 40.0 18-19 34.11058497810675 37.0 33.0 40.0 22.0 40.0 20-21 34.17762709590074 37.0 33.0 40.0 22.0 40.0 22-23 34.349426393937506 37.0 33.0 40.0 22.0 40.0 24-25 34.551607491306044 37.0 33.0 40.0 27.0 40.0 26-27 34.53046435347642 37.0 33.0 40.0 27.0 40.0 28-29 34.564606817379364 37.0 33.0 40.0 27.0 40.0 30-31 34.43609128520592 37.0 33.0 40.0 27.0 40.0 32-33 34.2443959697835 37.0 33.0 40.0 22.0 40.0 34-35 34.07928065260691 37.0 33.0 40.0 22.0 40.0 36-37 33.84527132483191 37.0 33.0 40.0 22.0 40.0 38-39 33.48303835609866 37.0 33.0 40.0 22.0 40.0 40-41 33.46830614262807 37.0 33.0 40.0 22.0 40.0 42-43 33.327027327389686 37.0 33.0 40.0 22.0 40.0 44-45 33.17567268977707 37.0 33.0 40.0 22.0 40.0 46-47 33.037324687231305 37.0 33.0 38.5 22.0 40.0 48-49 32.84916758060321 37.0 33.0 37.0 22.0 40.0 50-51 32.63223665321562 37.0 30.0 37.0 22.0 40.0 52-53 32.44297007885467 37.0 27.0 37.0 22.0 40.0 54-55 32.213449503850526 37.0 27.0 37.0 22.0 40.0 56-57 31.924122714627657 37.0 27.0 37.0 15.0 40.0 58-59 31.620674200302403 37.0 27.0 37.0 15.0 40.0 60-61 31.342101657979583 33.0 27.0 37.0 15.0 40.0 62-63 30.994498857922913 33.0 27.0 37.0 15.0 40.0 64-65 30.77723715172646 33.0 27.0 37.0 15.0 38.5 66-67 30.557771690281587 33.0 27.0 37.0 15.0 37.0 68-69 30.292697828216916 33.0 27.0 37.0 15.0 37.0 70-71 29.968561582580758 33.0 27.0 37.0 15.0 37.0 72-73 29.561065891138725 33.0 27.0 37.0 15.0 37.0 74-75 29.271825966492575 33.0 27.0 37.0 15.0 37.0 76-77 29.029590460226707 33.0 27.0 37.0 15.0 37.0 78-79 28.75742987301842 33.0 27.0 37.0 15.0 37.0 80-81 28.587108116131287 33.0 27.0 37.0 15.0 37.0 82-83 28.408422059516948 33.0 27.0 37.0 15.0 37.0 84-85 28.210461249965327 33.0 24.5 37.0 6.0 37.0 86-87 27.864566036943778 33.0 22.0 33.0 6.0 37.0 88-89 27.668932485889897 33.0 22.0 33.0 6.0 37.0 90-91 27.46398737080885 33.0 22.0 33.0 6.0 37.0 92-93 27.106015545292145 33.0 22.0 33.0 6.0 37.0 94-95 26.955333522492584 33.0 22.0 33.0 6.0 37.0 96-97 26.735614588900972 33.0 22.0 33.0 6.0 37.0 98-99 25.680263674752037 30.0 18.5 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7094588355237406 1101 2 0.783267044438233 1101 3 0.7811725782070802 1101 4 0.9162442643137467 1101 5 0.7868603500844245 1101 6 0.8708932106831213 1101 7 0.8040655387537043 1101 8 0.8922147532868507 1101 9 0.7573242308398562 1101 10-11 0.6876234741777054 1101 12-13 0.8906634604901313 1101 14-15 1.0810059712421989 1101 16-17 0.9582191174891221 1101 18-19 0.8950659673734336 1101 20-21 0.792207747622534 1101 22-23 0.7962015372595772 1101 24-25 0.7201996925637317 1101 26-27 0.7986920917860161 1101 28-29 0.8170482459627735 1101 30-31 1.0744754164165116 1101 32-33 1.2039850505787442 1101 34-35 1.207442821839372 1101 36-37 1.2794669507558396 1101 38-39 1.3978653440310538 1101 40-41 1.1427801591191624 1101 42-43 1.0030866512460719 1101 44-45 1.033594804097632 1101 46-47 1.1560832800046725 1101 48-49 0.8930617728443409 1101 50-51 0.9014322890323214 1101 52-53 1.0512765246747904 1101 54-55 0.9534133198134995 1101 56-57 1.0830008791474697 1101 58-59 1.0318019175768605 1101 60-61 0.8344154999959201 1101 62-63 0.9321873085349317 1101 64-65 1.0790067336928821 1101 66-67 1.0384694837791706 1101 68-69 1.1616335994993037 1101 70-71 1.072105660923576 1101 72-73 0.9058404452503908 1101 74-75 0.9068285293723335 1101 76-77 0.9119595369746278 1101 78-79 0.9299274385073808 1101 80-81 0.9640356561117116 1101 82-83 0.9671083060196786 1101 84-85 1.036915049202996 1101 86-87 0.9471674541419688 1101 88-89 0.9306185037380033 1101 90-91 0.793643019956292 1101 92-93 0.8759632445002019 1101 94-95 0.8776100783851923 1101 96-97 0.7261561248619479 1101 98-99 0.824129297575471 1102 1 0.5302389408325325 1102 2 0.5935014631474438 1102 3 0.5881461514444872 1102 4 0.5055659636392349 1102 5 0.42073155460685996 1102 6 0.5925300778925617 1102 7 0.5216405283581196 1102 8 0.6146381478704441 1102 9 0.4623847626212694 1102 10-11 0.5443297177406592 1102 12-13 0.5666564096631532 1102 14-15 0.7677031752212642 1102 16-17 0.6095304627785794 1102 18-19 0.5641053077926799 1102 20-21 0.6731134102441345 1102 22-23 0.6359663130668736 1102 24-25 0.5232834013149485 1102 26-27 0.6142503627024567 1102 28-29 0.6013991340719187 1102 30-31 0.7448132961489833 1102 32-33 0.8940365028049087 1102 34-35 0.9514688293871743 1102 36-37 1.0181063177499894 1102 38-39 0.9528696161216814 1102 40-41 0.7175774619243924 1102 42-43 0.5756824859153156 1102 44-45 0.48659232914362605 1102 46-47 0.5128129461796078 1102 48-49 0.42117806733927665 1102 50-51 0.4470574780635488 1102 52-53 0.611975837567087 1102 54-55 0.4734898443787543 1102 56-57 0.5609880449490419 1102 58-59 0.36927724283169994 1102 60-61 0.34645278455482753 1102 62-63 0.3702535316325388 1102 64-65 0.550208170457406 1102 66-67 0.4963473606119031 1102 68-69 0.6207485748182009 1102 70-71 0.6497217320028312 1102 72-73 0.4690152723530616 1102 74-75 0.5180726731137071 1102 76-77 0.4381221706318925 1102 78-79 0.4311379651227938 1102 80-81 0.4908483543106108 1102 82-83 0.4043939368846452 1102 84-85 0.4532557322602635 1102 86-87 0.5738025250626713 1102 88-89 0.32831549926036274 1102 90-91 0.49047105625658816 1102 92-93 0.5393737693956417 1102 94-95 0.5494146951016248 1102 96-97 0.2830070889772358 1102 98-99 0.4516722028950646 1103 1 0.5627038813402727 1103 2 0.6780262249127347 1103 3 0.5093923542798535 1103 4 0.6237029262745466 1103 5 0.5019923769833312 1103 6 0.8043134532110514 1103 7 0.590733152488383 1103 8 0.7101426179909396 1103 9 0.6796513654076506 1103 10-11 0.618627787263172 1103 12-13 0.6356729465722495 1103 14-15 0.8937398654838162 1103 16-17 0.6665147689308881 1103 18-19 0.623002544136888 1103 20-21 0.6706607180548687 1103 22-23 0.6137159808018424 1103 24-25 0.4799273434648015 1103 26-27 0.6785831801719269 1103 28-29 0.6488388942821715 1103 30-31 0.8993568471850253 1103 32-33 0.9853998040985488 1103 34-35 1.0761066242292827 1103 36-37 1.205037687586234 1103 38-39 1.1949053155562055 1103 40-41 1.0090798701486605 1103 42-43 0.8925363316438322 1103 44-45 0.7946417364519149 1103 46-47 0.770376405770655 1103 48-49 0.8050361191393947 1103 50-51 0.7505744857988716 1103 52-53 0.8713996634490684 1103 54-55 0.7498700260909246 1103 56-57 0.8112753622902389 1103 58-59 0.7623318933366328 1103 60-61 0.5629345870102753 1103 62-63 0.847619479891911 1103 64-65 0.8491543826841088 1103 66-67 0.8251198684862935 1103 68-69 0.8971906008234498 1103 70-71 0.9343178493386475 1103 72-73 0.6659038432398425 1103 74-75 0.7563418976783254 1103 76-77 0.6220872033138676 1103 78-79 0.7036619415180319 1103 80-81 0.6088138021064715 1103 82-83 0.7366395303026003 1103 84-85 0.8908059948039053 1103 86-87 0.803529036255334 1103 88-89 0.7736062834801842 1103 90-91 0.667934342185859 1103 92-93 0.6452161165534278 1103 94-95 0.8457101035054464 1103 96-97 0.6763739444661461 1103 98-99 0.7065982320230866 1104 1 0.3277006344097195 1104 2 0.3014805791237727 1104 3 0.3128809348191659 1104 4 0.26678850752853833 1104 5 0.2037317998250998 1104 6 0.3354085710621675 1104 7 0.22613808093719712 1104 8 0.27394412072025887 1104 9 0.3050796397026474 1104 10-11 0.2961898647763732 1104 12-13 0.2530203111568383 1104 14-15 0.35186665960586083 1104 16-17 0.3057930569971461 1104 18-19 0.40013009983332637 1104 20-21 0.22950197045593512 1104 22-23 0.20854514001896973 1104 24-25 0.1795359903910807 1104 26-27 0.3695877282490514 1104 28-29 0.2764589208986976 1104 30-31 0.5196422980869286 1104 32-33 0.6000669670054251 1104 34-35 0.6416728366818205 1104 36-37 0.7844063970292794 1104 38-39 0.7557982778490739 1104 40-41 0.5330351094005508 1104 42-43 0.4651870260239761 1104 44-45 0.3872606678971593 1104 46-47 0.4155475759952907 1104 48-49 0.24791602188581408 1104 50-51 0.16039383892957204 1104 52-53 0.23812166742848717 1104 54-55 0.07217099280959616 1104 56-57 0.3146584621057471 1104 58-59 0.31301995175526187 1104 60-61 0.18725489819324892 1104 62-63 0.20593034589043313 1104 64-65 0.39023850133537863 1104 66-67 0.3687322449307935 1104 68-69 0.39842621519969157 1104 70-71 0.4449383692274367 1104 72-73 0.23966839210051205 1104 74-75 0.26289571124871713 1104 76-77 0.29056119937718705 1104 78-79 0.23579865925708177 1104 80-81 0.11555930032927009 1104 82-83 0.2837119801859025 1104 84-85 0.3317422059742974 1104 86-87 0.1429170788845049 1104 88-89 0.10540836555214028 1104 90-91 0.056119551378408516 1104 92-93 0.12958566295663587 1104 94-95 0.27482689387601056 1104 96-97 0.06381108341578212 1104 98-99 0.01188262633698045 1105 1 0.2455750974728197 1105 2 0.2673359268209623 1105 3 0.26305916783594085 1105 4 0.15908903554348086 1105 5 0.13298844182437364 1105 6 0.39026427975998246 1105 7 0.20729907484906107 1105 8 0.42487434512955247 1105 9 0.18073612493459734 1105 10-11 0.24988725026025804 1105 12-13 0.2816917552417877 1105 14-15 0.3778646378845778 1105 16-17 0.1770094038474923 1105 18-19 0.22986059932156166 1105 20-21 0.24009279685879648 1105 22-23 0.14641429702067654 1105 24-25 0.033268577852751946 1105 26-27 0.22471819199057563 1105 28-29 0.19529822880219427 1105 30-31 0.4574293613085558 1105 32-33 0.49955694320176036 1105 34-35 0.5911247694190962 1105 36-37 0.6944683692940643 1105 38-39 0.49780312250101844 1105 40-41 0.39704038042192025 1105 42-43 0.2410132639969973 1105 44-45 0.2637439652874676 1105 46-47 0.17834069760833415 1105 48-49 0.023935392248894516 1105 50-51 0.043407007502146655 1105 52-53 0.21264483595393813 1105 54-55 0.073831897501897 1105 56-57 0.2453699572372443 1105 58-59 0.1907090972379244 1105 60-61 0.03190915911955372 1105 62-63 0.16164506529342404 1105 64-65 0.24636659001079764 1105 66-67 0.2131789231361303 1105 68-69 0.2923935590713391 1105 70-71 0.2587599966394549 1105 72-73 0.12898550464907999 1105 74-75 0.18925217257100257 1105 76-77 0.12340635248738607 1105 78-79 0.13639749039240812 1105 80-81 0.07685391600199765 1105 82-83 0.18320123496989638 1105 84-85 0.19974248187770982 1105 86-87 0.10810268355760755 1105 88-89 0.03222492599942939 1105 90-91 -0.007997928707666802 1105 92-93 -0.041428408706405406 1105 94-95 0.04855465376407153 1105 96-97 -0.14404390445860216 1105 98-99 -0.19927239604741587 1106 1 0.1706085513374198 1106 2 0.13086947375961344 1106 3 0.0756434851034058 1106 4 -0.04134998465902129 1106 5 0.00972814844236325 1106 6 0.04069783112685599 1106 7 0.1745892652683878 1106 8 0.07901678515452204 1106 9 0.10519592836818958 1106 10-11 0.11525738484647974 1106 12-13 0.056554954834659554 1106 14-15 0.1610589996639078 1106 16-17 0.05970611102458889 1106 18-19 0.23166532960368613 1106 20-21 0.0892298355099328 1106 22-23 0.10978489865460972 1106 24-25 0.040340578940742944 1106 26-27 0.18143044448728318 1106 28-29 0.126159343919781 1106 30-31 0.33458703336602724 1106 32-33 0.36442443290047777 1106 34-35 0.35373428428682985 1106 36-37 0.3934656022564056 1106 38-39 0.4235659698822829 1106 40-41 0.34972714580628406 1106 42-43 0.16121766555932737 1106 44-45 0.08616149011206886 1106 46-47 0.14021808417849968 1106 48-49 -0.08774275543853349 1106 50-51 -0.05258102098355977 1106 52-53 0.09816700170173931 1106 54-55 -0.029885568753662994 1106 56-57 -0.04016022404735864 1106 58-59 0.03024545006469026 1106 60-61 -0.03315241142982828 1106 62-63 0.07011394541109794 1106 64-65 0.2301249034070736 1106 66-67 0.13921224949865163 1106 68-69 0.0455760677612389 1106 70-71 0.18018236330432558 1106 72-73 -0.019802123986380593 1106 74-75 0.14196363521998734 1106 76-77 0.1463261329688592 1106 78-79 0.07730816284802344 1106 80-81 -0.0031596661138060256 1106 82-83 -0.029494381925726287 1106 84-85 0.0358273248026002 1106 86-87 -0.04648321070391148 1106 88-89 -0.07174848118276955 1106 90-91 -0.1975938403568982 1106 92-93 -0.16129171352225313 1106 94-95 -0.0765963274188195 1106 96-97 -0.20866184648906128 1106 98-99 -0.2104026202868141 1107 1 0.07784447290021745 1107 2 0.1723467571641777 1107 3 0.08997623307869418 1107 4 0.08042944561717746 1107 5 0.0901394227175274 1107 6 0.15235468191838208 1107 7 0.005732526137350646 1107 8 0.05801943318207492 1107 9 0.013131021543550503 1107 10-11 0.12042456100653709 1107 12-13 0.15348519969426633 1107 14-15 0.2787384620742017 1107 16-17 0.04189751238790507 1107 18-19 0.29387142514175224 1107 20-21 0.20619527889683553 1107 22-23 0.07226293990969168 1107 24-25 0.16785328467120308 1107 26-27 0.09900493307322478 1107 28-29 0.20555808732135716 1107 30-31 0.39447546157690283 1107 32-33 0.4079015680157241 1107 34-35 0.36750895014333196 1107 36-37 0.5771172496736483 1107 38-39 0.44361827598155656 1107 40-41 0.36932386596276245 1107 42-43 0.1298824146616866 1107 44-45 0.23759436973676884 1107 46-47 0.07006644186438393 1107 48-49 -0.03776687519603428 1107 50-51 -0.013424183909357623 1107 52-53 0.13664122035620352 1107 54-55 0.030780681834862378 1107 56-57 0.16533064983413936 1107 58-59 0.06458632438543077 1107 60-61 -6.346271599078079E-4 1107 62-63 0.09188659662533283 1107 64-65 0.17924815704522956 1107 66-67 0.1629166135400517 1107 68-69 0.21456459987413723 1107 70-71 0.12840091992106295 1107 72-73 -0.16357491945291258 1107 74-75 0.009850058738990697 1107 76-77 0.1177975118090302 1107 78-79 0.12338601138384675 1107 80-81 0.05924552261348737 1107 82-83 0.06619305149013854 1107 84-85 0.13387083407038247 1107 86-87 -0.018028897672504485 1107 88-89 -0.07620719632578954 1107 90-91 -0.007670153347767439 1107 92-93 -0.08147887732505765 1107 94-95 -0.017106838973461436 1107 96-97 -0.20079182321110878 1107 98-99 -0.16947788231696137 1108 1 0.2159993102710267 1108 2 0.1934457893837731 1108 3 0.23945680499459954 1108 4 0.12379347880776947 1108 5 0.10506499474739428 1108 6 0.23492965638133967 1108 7 0.25125785640031495 1108 8 0.2111162535672122 1108 9 0.22100011536053898 1108 10-11 0.1404935257388118 1108 12-13 0.12938037241092815 1108 14-15 0.2440227993545605 1108 16-17 0.12866526799546563 1108 18-19 0.49848292445521025 1108 20-21 0.2899375462593028 1108 22-23 0.24092075437394556 1108 24-25 0.25006823171696624 1108 26-27 0.2551549361201708 1108 28-29 0.22758267793491171 1108 30-31 0.5217495485986205 1108 32-33 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36-37 -0.45074856035841293 2216 38-39 -8.760229997679293 2216 40-41 -2.367838794073993 2216 42-43 -1.0069069083561928 2216 44-45 -1.0762804459541826 2216 46-47 -0.8236902022334078 2216 48-49 -0.6921854359857562 2216 50-51 -0.6976151674076405 2216 52-53 -0.8535367395268452 2216 54-55 -0.8201865369253696 2216 56-57 -0.7791039079501338 2216 58-59 -0.8507359406057411 2216 60-61 -0.77038964025148 2216 62-63 -0.9184744085815169 2216 64-65 -0.9162430957676087 2216 66-67 -0.8904272546477685 2216 68-69 -0.7815463131304696 2216 70-71 -0.6364647931363763 2216 72-73 -0.7012124573360126 2216 74-75 -0.721249389116533 2216 76-77 -0.7843716042940265 2216 78-79 -0.8610637950868814 2216 80-81 -0.867654221663674 2216 82-83 -0.9264409090221015 2216 84-85 -0.9219648695415792 2216 86-87 -0.7646540586923685 2216 88-89 -0.602640599577569 2216 90-91 -0.6277183657695318 2216 92-93 -0.5812575690228847 2216 94-95 -0.491909869773977 2216 96-97 -0.5073087773528009 2216 98-99 -0.38883998784215024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 147.0 8 925.0 9 1815.0 10 3734.0 11 10919.0 12 29581.0 13 58737.0 14 78550.0 15 79234.0 16 76676.0 17 71535.0 18 66818.0 19 65799.0 20 66828.0 21 70611.0 22 76494.0 23 83301.0 24 90991.0 25 97186.0 26 102650.0 27 112788.0 28 132399.0 29 157437.0 30 190937.0 31 235941.0 32 295850.0 33 373088.0 34 475781.0 35 618082.0 36 784343.0 37 675125.0 38 151523.0 39 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04763890416802 20.649578486517058 12.679887951234386 22.622894658080533 2 14.015581218351647 23.165035009857036 38.176758687525336 24.642625084265983 3 17.985522384115445 32.02590099946641 28.084843034837853 21.903733581580294 4 12.055172780858383 20.566814320829582 35.717908083596896 31.66010481471514 5 11.753943520376762 42.14247801741814 32.14590406905481 13.957674393150295 6 27.42473048554956 38.91647357180393 18.658792194444715 15.0000037482018 7 23.81974574684545 36.127126952097534 22.08336308484682 17.969764216210198 8 26.86203169029658 35.33469193154584 19.118434181201568 18.684842196956012 9 26.936433496030283 18.17394355058161 19.176119006906436 35.71350394648167 10-11 23.615088500206575 30.36059672502711 23.931652283409434 22.09266249135688 12-13 25.651749591797053 28.43681580618032 23.9282780980671 21.983156503955527 14-15 24.93614296180085 27.046203652459532 22.75352448307613 25.26412890266349 16-17 22.18905685221595 29.35983277007178 25.33765239031486 23.11345798739741 18-19 23.326390414198787 29.540852775880772 26.538046497192973 20.594710312727468 20-21 25.135188268428223 28.20652104652793 25.141269725849025 21.517020959194827 22-23 24.52838480692495 28.910848355893293 22.783699781001918 23.777067056179835 24-25 23.44396423363396 29.768053868070904 22.96230723084284 23.825674667452297 26-27 24.076152811266375 28.126438697759077 23.395660777452708 24.40174771352184 28-29 23.072503722500585 28.38699762640806 25.095370043264403 23.445128607826952 30-31 24.318624393093515 28.813478320882922 24.468387262303455 22.399510023720108 32-33 22.161373971452214 30.116716881934973 24.19250840609495 23.52940074051786 34-35 21.481520143496187 30.668917337329415 25.436726499847918 22.41283601932648 36-37 23.34375333824348 29.24176660313462 24.54908178391983 22.865398274702066 38-39 24.054052584385794 28.803937547369564 24.13533790881268 23.006671959431966 40-41 22.32651760781153 29.470397511104444 24.93699983132485 23.26608504975917 42-43 23.70289388175667 28.991048895795597 24.317366326591426 22.988690895856315 44-45 23.482025123447027 29.325040686730542 24.764547333416793 22.428386856405638 46-47 22.04418131873023 29.292189017593724 26.395660299830254 22.267969363845797 48-49 23.059282001152198 29.14530970114429 26.037512795438282 21.757895502265228 50-51 21.414658802380576 29.73573854747096 25.112765496494283 23.736837153654186 52-53 20.805323281137024 30.082365014818546 23.52769420313268 25.58461750091175 54-55 21.39884015643495 29.514390471171453 23.390081733288454 25.696687639105136 56-57 23.286247295544374 27.44903572903428 23.596712101614912 25.66800487380644 58-59 22.099315550175636 27.589213536294732 25.995178125342317 24.316292788187315 60-61 22.726684088334753 28.29629542171456 24.687427612744205 24.289592877206477 62-63 21.24704196543745 27.99147321412675 24.85760110167168 25.90388371876412 64-65 20.059360705776566 28.38081371432084 25.520090227373203 26.03973535252939 66-67 23.141325981761003 26.745176936253472 24.780343040057573 25.33315404192795 68-69 22.6979844162776 26.28438456740702 25.1290147501117 25.888616266203684 70-71 21.53302044588242 27.068656387471187 25.23479209001833 26.163531076628065 72-73 23.18713586742524 26.070735074816376 25.89757443920749 24.844554618550895 74-75 23.39170759618194 25.894922203778663 26.143497909122885 24.569872290916507 76-77 21.796559448024396 26.53073865118608 27.09043930457311 24.582262596216413 78-79 22.41492257289061 26.59426324326994 26.759437203487817 24.231376980351634 80-81 21.590073412280457 26.86949998238345 27.099227270716874 24.441199334619217 82-83 22.078134800982255 26.250594793999344 26.30067087328222 25.370599531736183 84-85 22.567346840480358 26.926807482908355 25.246040169858077 25.259805506753214 86-87 22.38655692431556 25.636257255581636 26.209301087803127 25.767884732299677 88-89 23.335011278339216 24.31228930420631 26.60745382402792 25.745245593426553 90-91 23.247614279343644 25.231642537948183 26.214960789368313 25.30578239333986 92-93 22.61560953632495 25.2855942361652 26.5970244525189 25.50177177499095 94-95 21.278869587315484 25.932055971147843 27.221502983943452 25.567571457593218 96-97 22.628305768434608 25.807938248529577 25.832021262021126 25.73173472101469 98-99 22.805639196244734 26.412797756376253 25.12256229508135 25.659000752297658 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2301.0 1 1846.5 2 1278.5 3 1041.0 4 857.0 5 743.5 6 643.5 7 596.5 8 601.0 9 590.0 10 552.0 11 554.0 12 569.5 13 574.0 14 601.5 15 745.0 16 899.5 17 996.0 18 1254.0 19 1595.0 20 2089.5 21 2815.0 22 3985.5 23 5658.5 24 7703.5 25 10756.0 26 14771.5 27 19309.0 28 26791.0 29 37984.0 30 47757.5 31 58530.5 32 70530.5 33 79270.0 34 87744.5 35 98623.5 36 103297.0 37 113289.5 38 131519.0 39 125766.5 40 116139.5 41 122193.5 42 128766.5 43 140937.5 44 166345.5 45 207241.5 46 238003.5 47 275172.5 48 318059.5 49 365602.5 50 423440.5 51 363285.5 52 253188.5 53 211821.5 54 173129.5 55 149778.0 56 128581.0 57 93078.0 58 81924.0 59 68684.0 60 47871.5 61 38120.5 62 29613.5 63 22061.0 64 14345.0 65 9945.5 66 9773.0 67 8330.5 68 7059.5 69 5469.0 70 3970.0 71 3587.0 72 3378.0 73 2931.5 74 2577.0 75 2897.0 76 3932.0 77 3893.0 78 2883.0 79 2874.5 80 3417.0 81 3184.5 82 3302.5 83 2948.5 84 1915.5 85 1557.0 86 958.5 87 454.5 88 294.5 89 247.5 90 235.0 91 200.0 92 130.5 93 94.5 94 68.0 95 46.0 96 37.0 97 21.5 98 9.5 99 4.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.8741009000931804E-5 2 0.0020427699811015666 3 0.006465648105321472 4 0.0 5 0.005697266736283268 6 0.0 7 5.434892610270222E-4 8 0.0 9 0.0 10-11 9.370504500465902E-6 12-13 8.433454050419312E-5 14-15 0.0027174463051351117 16-17 9.370504500465901E-5 18-19 0.0 20-21 0.0 22-23 9.370504500465902E-6 24-25 7.683813690382038E-4 26-27 0.002258291584612282 28-29 0.0018178778730903848 30-31 0.0023145146116150777 32-33 5.903417835293517E-4 34-35 1.8741009000931804E-5 36-37 3.748201800186361E-5 38-39 0.052962091436633274 40-41 0.0035982737281789064 42-43 2.623741260130453E-4 44-45 0.0 46-47 7.402698555368062E-4 48-49 1.1244605400559083E-4 50-51 7.496403600372721E-4 52-53 1.8741009000931803E-4 54-55 0.0 56-57 4.0293169352003375E-4 58-59 9.370504500465902E-6 60-61 1.4992807200745443E-4 62-63 1.8741009000931804E-5 64-65 7.308993510363403E-4 66-67 0.0013493526480670899 68-69 0.0012743886120633627 70-71 1.8741009000931804E-5 72-73 0.0017803958550885214 74-75 0.0017429138370866576 76-77 7.496403600372722E-5 78-79 4.685252250232951E-5 80-81 0.0 82-83 2.0615109901024985E-4 84-85 4.77895729523761E-4 86-87 0.0 88-89 0.0 90-91 5.716007745284199E-4 92-93 0.0 94-95 0.0 96-97 0.0033921226291686564 98-99 0.004460360142221769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5335892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.303127826652354 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71683701289818 18.343485150213258 2 10.07567330949828 4.695894061416555 3 3.806428484058703 2.661050685810915 4 2.009834518563359 1.8734172278600099 5 1.1158226583487731 1.3001079019688246 6 0.7347829755293289 1.0273644962164752 7 0.47601125081172746 0.7764785717213252 8 0.3502774517267525 0.653004818590605 9 0.26837164066840835 0.5628508782789899 >10 1.7811822192606888 8.267525313997808 >50 0.2570397910276294 4.235922708982942 >100 0.30074741517088316 15.29885680645761 >500 0.05884935000627922 9.59915326313669 >1k 0.04407476001877677 18.613509357435127 >5k 0.0027391093796979914 4.199982550845049 >10k+ 0.0013280530325808443 7.891396207067903 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 78333 1.468039458069991 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 73920 1.3853353853488788 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31168 0.5841197685410424 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 24807 0.46490821028611523 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22113 0.41441993203760497 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 21909 0.4105967662014149 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 21249 0.3982277002607999 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 21180 0.39693457063973553 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 21082 0.3950979517576443 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 20876 0.39123730390345235 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14562 0.2729065730715689 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13517 0.2533222186655952 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11959 0.22412372664214344 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 11198 0.20986181879243432 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 10087 0.1890405577923991 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 10025 0.18787861523434132 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9763 0.1829684708760972 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 8666 0.162409584002075 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 8417 0.15774307276084298 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8132 0.15240188519557743 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7976 0.14947828779143205 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7262 0.13609720736476677 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 7250 0.13587231525675555 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7120 0.13343598408663446 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 7109 0.1332298329876242 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 7064 0.13238648758258226 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 6907 0.12944414916943597 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6879 0.1289194009174099 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 6630 0.12425288967617784 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6545 0.12265990391109864 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 6512 0.1220414506140679 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 6367 0.11932400430893278 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6335 0.11872429202090297 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6307 0.11819954376887688 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6301 0.11808709771487129 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 6161 0.11546335645474083 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 6121 0.11471371609470357 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6022 0.11285835620361133 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5958 0.1116589316275517 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 5909 0.11074062218650602 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 5824 0.10914763642142682 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5812 0.10892274431341564 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5538 0.10378770784716033 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 5529 0.10361903876615194 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 5462 0.1023633911630895 No Hit CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA 5388 0.10097655649702056 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5359 0.10043306723599355 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.748201800186361E-5 0.0 2 0.0 0.0 0.0 3.748201800186361E-5 0.0 3 0.0 0.0 0.0 3.748201800186361E-5 0.0 4 0.0 0.0 0.0 3.748201800186361E-5 0.0 5 0.0 0.0 0.0 1.6866908100838623E-4 0.0 6 0.0 0.0 0.0 1.6866908100838623E-4 0.0 7 0.0 0.0 0.0 1.6866908100838623E-4 0.0 8 1.8741009000931804E-5 0.0 0.0 1.6866908100838623E-4 0.0 9 1.8741009000931804E-5 0.0 0.0 1.6866908100838623E-4 0.0 10-11 3.748201800186361E-5 0.0 0.0 1.7803958550885213E-4 0.0 12-13 4.685252250232951E-5 0.0 0.0 1.9678059450978393E-4 0.0 14-15 5.6223027002795406E-5 0.0 0.0 2.9985614401490887E-4 0.0 16-17 6.559353150326132E-5 0.0 0.0 4.5915472052282915E-4 0.0 18-19 7.496403600372722E-5 0.0 0.0 5.060072430251586E-4 0.0 20-21 1.4055756750698854E-4 0.0 0.0 0.001030755495051249 0.0 22-23 1.7803958550885213E-4 0.0 0.0 0.004525953673725031 0.0 24-25 2.530036215125793E-4 0.0 0.0 0.012809479652136888 0.0 26-27 3.1859715301584067E-4 0.0 0.0 0.02233928272911071 0.0 28-29 3.6544967551817016E-4 0.0 0.0 0.05004786453698838 0.0 30-31 3.8419068451910196E-4 0.0 0.0 0.12838528216088332 0.0 32-33 4.5915472052282915E-4 0.0 0.0 0.2560396649707303 0.0 34-35 5.060072430251586E-4 0.0 0.0 0.4135016225965593 0.0 36-37 5.060072430251586E-4 0.0 0.0 0.6491510697742758 0.0 38-39 5.060072430251586E-4 0.0 0.0 1.0064952588995428 0.0 40-41 5.247482520260904E-4 0.0 0.0 1.4860308267108855 0.0 42-43 5.434892610270222E-4 0.0 0.0 1.8593048734869446 0.0 44-45 5.622302700279541E-4 0.0 0.0 2.2554710627576418 0.0 46-47 5.622302700279541E-4 0.0 0.0 2.6713059409748174 0.0 48-49 5.622302700279541E-4 0.0 0.0 3.100015892375633 0.0 50-51 5.622302700279541E-4 0.0 0.0 3.602293674609606 0.0 52-53 5.809712790288859E-4 0.0 0.0 4.124933563123092 0.0 54-55 5.809712790288859E-4 0.0 0.0 4.581848733070309 0.0 56-57 5.809712790288859E-4 0.0 0.0 5.071317035652146 0.0 58-59 5.809712790288859E-4 0.0 0.0 5.626182089142734 0.0 60-61 5.809712790288859E-4 0.0 0.0 6.153010593167927 0.0 62-63 5.809712790288859E-4 0.0 0.0 6.682500320471254 0.0 64-65 5.997122880298177E-4 0.0 0.0 7.2475511123538485 0.0 66-67 5.997122880298177E-4 0.0 0.0 7.838633165738736 0.0 68-69 5.997122880298177E-4 0.0 0.0 8.511360424836184 0.0 70-71 5.997122880298177E-4 0.0 0.0 9.338654155668817 0.0 72-73 5.997122880298177E-4 0.0 0.0 10.027854761678086 0.0 74-75 5.997122880298177E-4 0.0 0.0 10.68261876364814 0.0 76-77 6.184532970307495E-4 0.0 0.0 11.392275180981924 0.0 78-79 6.184532970307495E-4 0.0 0.0 12.056259759380438 0.0 80-81 6.184532970307495E-4 0.0 0.0 12.796538985421744 0.0 82-83 6.184532970307495E-4 1.8741009000931804E-5 0.0 13.646068923433983 0.0 84-85 6.184532970307495E-4 2.8111513501397706E-5 0.0 14.430239592555472 0.0 86-87 6.184532970307495E-4 3.748201800186361E-5 0.0 15.183094035636405 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 1490 0.0 48.37303 1 TACATGA 2025 0.0 42.25233 2 GTACAAG 3095 0.0 41.467354 1 GTAACAA 1630 0.0 37.08631 1 GTACAAA 3115 0.0 35.379215 1 TACAAGA 1755 0.0 34.444836 2 TACATAG 1345 0.0 33.189972 2 TACAAGG 2250 0.0 31.000353 2 AGGGCTA 2750 0.0 28.405733 5 TATTCGC 705 0.0 27.041088 9 TACATAA 1565 0.0 26.741518 2 TAGTCCG 70 0.009314461 26.569988 5 GGAAACA 4085 0.0 26.522945 1 TAACCAC 1430 0.0 26.337732 5 GTACTAG 4495 0.0 26.27616 1 GTGATCG 3455 0.0 25.165092 8 AGGTGAT 3575 0.0 25.100723 6 CTATTGA 3170 0.0 25.082235 9 GAACAAA 3000 0.0 24.955284 1 ATAAGGT 3670 0.0 24.83407 3 >>END_MODULE