##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765260_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5335892 Sequences flagged as poor quality 0 Sequence length 99 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2877234021978 33.0 33.0 33.0 33.0 33.0 2 32.10506678171147 33.0 33.0 33.0 27.0 33.0 3 32.161164993594326 33.0 33.0 33.0 27.0 33.0 4 36.07068527623873 37.0 37.0 37.0 33.0 37.0 5 36.10882604070697 37.0 37.0 37.0 33.0 37.0 6 36.14881916650487 37.0 37.0 37.0 33.0 37.0 7 36.052653427018384 37.0 37.0 37.0 33.0 37.0 8 36.03758959139353 37.0 37.0 37.0 33.0 37.0 9 35.73593543497507 37.0 37.0 37.0 33.0 37.0 10-11 35.91951683055054 37.0 37.0 37.0 33.0 37.0 12-13 35.842976488279746 37.0 37.0 37.0 33.0 37.0 14-15 37.131726522950615 37.0 37.0 40.0 33.0 40.0 16-17 37.28456507365591 37.0 37.0 40.0 33.0 40.0 18-19 37.40990316145829 37.0 37.0 40.0 33.0 40.0 20-21 37.45784069467673 37.0 37.0 40.0 33.0 40.0 22-23 37.39219618762898 37.0 37.0 40.0 33.0 40.0 24-25 37.326970635837455 37.0 37.0 40.0 33.0 40.0 26-27 37.13965115860666 37.0 37.0 40.0 33.0 40.0 28-29 37.03896686814501 37.0 37.0 40.0 33.0 40.0 30-31 36.80425475253247 37.0 37.0 40.0 33.0 40.0 32-33 36.47364920429424 37.0 37.0 40.0 33.0 40.0 34-35 36.32234751003206 37.0 37.0 40.0 33.0 40.0 36-37 36.09109892029299 37.0 37.0 40.0 33.0 40.0 38-39 35.857724256787805 37.0 37.0 40.0 33.0 40.0 40-41 35.5891609125522 37.0 37.0 40.0 27.0 40.0 42-43 35.28316202801706 37.0 37.0 40.0 27.0 40.0 44-45 34.84588668586245 37.0 33.0 40.0 24.5 40.0 46-47 34.32420474402406 37.0 33.0 40.0 22.0 40.0 48-49 33.84602283929285 37.0 33.0 40.0 18.5 40.0 50-51 33.45549619445072 37.0 33.0 40.0 15.0 40.0 52-53 33.27696353299505 37.0 33.0 40.0 15.0 40.0 54-55 33.171474047825555 37.0 33.0 40.0 15.0 40.0 56-57 32.98509386996589 37.0 33.0 38.5 15.0 40.0 58-59 32.76816922081632 37.0 33.0 37.0 15.0 40.0 60-61 32.55077098636929 37.0 33.0 37.0 15.0 40.0 62-63 32.18645495448558 37.0 33.0 37.0 15.0 40.0 64-65 32.07141467630905 37.0 33.0 37.0 15.0 40.0 66-67 31.910983018396923 37.0 33.0 37.0 15.0 40.0 68-69 31.669532572998104 37.0 33.0 37.0 15.0 38.5 70-71 31.45715767860369 35.0 33.0 37.0 15.0 37.0 72-73 31.231931886927246 33.0 33.0 37.0 15.0 37.0 74-75 31.012045502420214 33.0 33.0 37.0 15.0 37.0 76-77 30.82401030605567 33.0 27.0 37.0 15.0 37.0 78-79 30.6374743904112 33.0 27.0 37.0 15.0 37.0 80-81 30.560821789496487 33.0 27.0 37.0 15.0 37.0 82-83 30.407291601854013 33.0 27.0 37.0 15.0 37.0 84-85 30.245616196879546 33.0 27.0 37.0 15.0 37.0 86-87 30.151328025379826 33.0 27.0 37.0 15.0 37.0 88-89 30.050434866372857 33.0 27.0 37.0 15.0 37.0 90-91 29.968731188712216 33.0 27.0 37.0 15.0 37.0 92-93 29.825105905441866 33.0 27.0 37.0 15.0 37.0 94-95 29.78097270334557 33.0 27.0 37.0 15.0 37.0 96-97 29.74195860785788 33.0 27.0 37.0 15.0 37.0 98-99 28.91125710565356 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1589516833264284 1101 2 0.38319584858222555 1101 3 0.27415458672841453 1101 4 0.23026901567985192 1101 5 0.10149854886451948 1101 6 0.1719122494151364 1101 7 0.17996964385203285 1101 8 0.18272700039617007 1101 9 0.42142336554683624 1101 10-11 0.3821189133580276 1101 12-13 0.3746812403667761 1101 14-15 0.6431368441967251 1101 16-17 0.48309069207081023 1101 18-19 0.30107847269201216 1101 20-21 0.3428014601212084 1101 22-23 0.25949971848757514 1101 24-25 0.35681385285742095 1101 26-27 0.24418289259791237 1101 28-29 0.2866091272500171 1101 30-31 0.36061943265936236 1101 32-33 0.4569661215396721 1101 34-35 0.4272318178324426 1101 36-37 0.49821334957351837 1101 38-39 0.3422607954532282 1101 40-41 0.2649708453490476 1101 42-43 0.5173585517366774 1101 44-45 0.5793140044899374 1101 46-47 0.6383835061450114 1101 48-49 0.6774399551852852 1101 50-51 0.6697262801010453 1101 52-53 0.7142612103768542 1101 54-55 0.6224060424713755 1101 56-57 0.7379389752373342 1101 58-59 0.7941794934988735 1101 60-61 0.6222797537780664 1101 62-63 0.6691628369452047 1101 64-65 0.6265708543675217 1101 66-67 0.6274273816717049 1101 68-69 0.5480517968585481 1101 70-71 0.5740881208800488 1101 72-73 0.7057739306300483 1101 74-75 0.5183568714582236 1101 76-77 0.5408331728865079 1101 78-79 0.6855281665639623 1101 80-81 0.7421119900543403 1101 82-83 0.7389277531558704 1101 84-85 0.7589591279349719 1101 86-87 0.5809924598353398 1101 88-89 0.7281038358352525 1101 90-91 0.6253110939618267 1101 92-93 0.7564932756964318 1101 94-95 0.6943196985851046 1101 96-97 0.6311993223722787 1101 98-99 0.694479936248527 1102 1 0.1518905304142919 1102 2 0.3961743072889661 1102 3 0.25544765662705515 1102 4 0.21625517759463264 1102 5 0.08595656324960999 1102 6 0.20620255971523704 1102 7 0.11751950963910218 1102 8 0.08700885372525136 1102 9 0.42463894042403894 1102 10-11 0.31454903827095393 1102 12-13 0.32478894198807495 1102 14-15 0.5769697367751476 1102 16-17 0.4277219689825884 1102 18-19 0.3625184972998312 1102 20-21 0.3934895671029821 1102 22-23 0.25667380381868554 1102 24-25 0.3592776330507945 1102 26-27 0.31998066040330997 1102 28-29 0.3243832596262024 1102 30-31 0.326359918476129 1102 32-33 0.35098449516312513 1102 34-35 0.3360766594253306 1102 36-37 0.3296791926524918 1102 38-39 0.26111052929847034 1102 40-41 0.32575783784448475 1102 42-43 0.24338104943559813 1102 44-45 0.36168228280592274 1102 46-47 0.30148476777440436 1102 48-49 0.2904218992735821 1102 50-51 0.2860432892112357 1102 52-53 0.2312009218151161 1102 54-55 0.2963015550320094 1102 56-57 0.4118287949071444 1102 58-59 0.23901975703853395 1102 60-61 0.2740545548872362 1102 62-63 0.38523647800068517 1102 64-65 0.2524755092263291 1102 66-67 0.3038910996066413 1102 68-69 0.3336723358880995 1102 70-71 0.29858843647211586 1102 72-73 0.34768390579814223 1102 74-75 0.2604280523369944 1102 76-77 0.24825919165051857 1102 78-79 0.3677807974365592 1102 80-81 0.3742050897740441 1102 82-83 0.35354502555621536 1102 84-85 0.37437021037033347 1102 86-87 0.31904268833223526 1102 88-89 0.3260784566856927 1102 90-91 0.32352332032909104 1102 92-93 0.38320161230519645 1102 94-95 0.39310926886996 1102 96-97 0.24580919098641374 1102 98-99 0.25167542624351213 1103 1 0.11159320884040369 1103 2 0.3285584798995842 1103 3 0.26120584757008913 1103 4 0.21512377935648175 1103 5 0.13925578911798198 1103 6 0.20208095617383037 1103 7 0.2126910250782572 1103 8 0.13473411717744455 1103 9 0.4675102139792884 1103 10-11 0.33307439506079817 1103 12-13 0.3211298138183949 1103 14-15 0.5916072689476692 1103 16-17 0.4588414775242029 1103 18-19 0.3337004510361652 1103 20-21 0.4298790190123256 1103 22-23 0.30128370411098615 1103 24-25 0.40118134701873487 1103 26-27 0.3349299927607987 1103 28-29 0.20750679612785916 1103 30-31 0.27798256879779615 1103 32-33 0.3923480723197983 1103 34-35 0.46910421358414567 1103 36-37 0.47001497313077323 1103 38-39 0.3629893994018829 1103 40-41 0.3773819754276886 1103 42-43 0.3217078136306384 1103 44-45 0.47181713364977895 1103 46-47 0.6670944952325328 1103 48-49 0.5285384846266226 1103 50-51 0.5221468240841034 1103 52-53 0.6014708453926616 1103 54-55 0.4814967569103743 1103 56-57 0.5420594277933048 1103 58-59 0.572856885227651 1103 60-61 0.5275639524011808 1103 62-63 0.5427655416699935 1103 64-65 0.3368728192739425 1103 66-67 0.41632072604852155 1103 68-69 0.3905533133451762 1103 70-71 0.39176541723762526 1103 72-73 0.4424948652605494 1103 74-75 0.45775400105693365 1103 76-77 0.3923973541700647 1103 78-79 0.6645323843386315 1103 80-81 0.5400499095041482 1103 82-83 0.5208921443647831 1103 84-85 0.6142150775968567 1103 86-87 0.4978805217711759 1103 88-89 0.5433914464400225 1103 90-91 0.38194688186231573 1103 92-93 0.30339719679358623 1103 94-95 0.5636900421044331 1103 96-97 0.5212568507144084 1103 98-99 0.5425582820879278 1104 1 0.14176075520561682 1104 2 0.3084490874258137 1104 3 0.22595516316224717 1104 4 0.22452296067885413 1104 5 0.09521301629865064 1104 6 0.18843256966681565 1104 7 0.16338371348926728 1104 8 0.09000402792543838 1104 9 0.4006126116030373 1104 10-11 0.210715360521057 1104 12-13 0.21124831194384086 1104 14-15 0.40192164543015707 1104 16-17 0.20821958941331786 1104 18-19 0.16868787882609126 1104 20-21 0.305045209027206 1104 22-23 0.20793075563686614 1104 24-25 0.3249265745650476 1104 26-27 0.2399192844194502 1104 28-29 0.16760633799604818 1104 30-31 0.14073592732303553 1104 32-33 0.23870400120311075 1104 34-35 0.32116902694797034 1104 36-37 0.1934255798497233 1104 38-39 0.059543304978532774 1104 40-41 0.05814036521131527 1104 42-43 0.20737447020629673 1104 44-45 0.21241240227244162 1104 46-47 0.3284007733834784 1104 48-49 0.45204295256926486 1104 50-51 0.39124405867276124 1104 52-53 0.3995574120040075 1104 54-55 0.362917918231183 1104 56-57 0.3353901018489651 1104 58-59 0.3226918823902949 1104 60-61 0.20518754055030541 1104 62-63 0.294801269184255 1104 64-65 0.2111576635281267 1104 66-67 0.35948883098077644 1104 68-69 0.18696834882991098 1104 70-71 0.2478002264700656 1104 72-73 0.37769040527199493 1104 74-75 0.21236679420074722 1104 76-77 0.24058597353008082 1104 78-79 0.40367607615209167 1104 80-81 0.36325755338413046 1104 82-83 0.33458230468123773 1104 84-85 0.24435537823769593 1104 86-87 0.3408818727792067 1104 88-89 0.27482400466514534 1104 90-91 0.22659140529364308 1104 92-93 0.22424418266854929 1104 94-95 0.4054482104794488 1104 96-97 0.2972452056899897 1104 98-99 0.2682936940504028 1105 1 0.15228215671251633 1105 2 0.2756659168862896 1105 3 0.24353050511169272 1105 4 0.1893564645992143 1105 5 -0.019379903966736833 1105 6 0.0947776415077044 1105 7 0.1088355620127004 1105 8 0.03136305394221495 1105 9 0.2992670598020979 1105 10-11 0.21249016738867255 1105 12-13 0.21881517656212424 1105 14-15 0.41792790081129993 1105 16-17 0.32250373783463004 1105 18-19 0.24636685092244193 1105 20-21 0.3110206159332165 1105 22-23 0.13383304372125338 1105 24-25 0.3271203526927735 1105 26-27 0.221468648457666 1105 28-29 0.1446662131593257 1105 30-31 0.18643509513708523 1105 32-33 0.21352133665337902 1105 34-35 0.357881620098496 1105 36-37 0.20891801297037915 1105 38-39 0.15802521048470908 1105 40-41 0.14562070304430108 1105 42-43 0.24923392818711676 1105 44-45 0.23969119663635752 1105 46-47 0.43553074921469914 1105 48-49 0.37909006315089044 1105 50-51 0.23389634145697613 1105 52-53 0.17360862226890816 1105 54-55 0.21405707984992972 1105 56-57 0.27395297779312955 1105 58-59 0.3219355257169667 1105 60-61 0.3013029485634533 1105 62-63 0.24689443237421216 1105 64-65 0.11273437645547801 1105 66-67 0.18154544653807747 1105 68-69 0.16937325637723788 1105 70-71 0.2407521309397609 1105 72-73 0.2688328161908231 1105 74-75 0.19494080669030822 1105 76-77 0.20417376764547868 1105 78-79 0.32687611555003926 1105 80-81 0.2943233576735018 1105 82-83 0.2893700901985774 1105 84-85 0.27615334980285766 1105 86-87 0.27641210790662996 1105 88-89 0.22315448719283282 1105 90-91 0.20486917907993174 1105 92-93 0.15817749060031616 1105 94-95 0.3895543629194691 1105 96-97 0.18520213943430264 1105 98-99 0.22838337443823775 1106 1 0.08789803627085035 1106 2 0.24866301265934965 1106 3 0.17622095337307542 1106 4 0.16585544514173733 1106 5 0.032217409232295324 1106 6 0.08624470390380168 1106 7 0.0650810427436852 1106 8 0.03574555849581884 1106 9 0.2760176024266343 1106 10-11 0.20802809566999514 1106 12-13 0.23049186318232984 1106 14-15 0.3936879023066453 1106 16-17 0.32023517680183744 1106 18-19 0.1811096701945729 1106 20-21 0.3309579166876162 1106 22-23 0.15389904474641014 1106 24-25 0.21338638465594784 1106 26-27 0.05094482724518201 1106 28-29 0.15546356532789218 1106 30-31 0.11708778306000056 1106 32-33 0.16000494941194887 1106 34-35 0.18579951114119098 1106 36-37 0.09789368560556966 1106 38-39 0.0850773335061632 1106 40-41 0.08261652138565267 1106 42-43 0.0975683674631398 1106 44-45 0.10205598419580042 1106 46-47 0.1996917330293897 1106 48-49 0.2820990032992583 1106 50-51 0.0890393685375983 1106 52-53 0.16387227940238347 1106 54-55 0.1602056129609366 1106 56-57 0.21377063834594168 1106 58-59 0.1679165429680296 1106 60-61 0.21205380945665553 1106 62-63 0.29000242213319893 1106 64-65 0.03532237267860694 1106 66-67 0.17839752926834862 1106 68-69 0.09274642373817343 1106 70-71 0.07953684746200196 1106 72-73 0.19430794515941585 1106 74-75 0.16912512851235562 1106 76-77 0.09860198051088531 1106 78-79 0.30696373717498915 1106 80-81 0.17361004141528724 1106 82-83 0.09750883447868475 1106 84-85 0.10616178695777378 1106 86-87 0.14530572375304374 1106 88-89 -0.0440848534394398 1106 90-91 0.03864044528740962 1106 92-93 -0.052938263845057065 1106 94-95 0.14245019162917671 1106 96-97 0.24389022538525396 1106 98-99 0.06984896777916205 1107 1 0.08439037988254938 1107 2 0.21206799006868238 1107 3 0.19266872888124453 1107 4 0.2164214904194992 1107 5 0.08491148863070919 1107 6 0.1088173455542858 1107 7 0.07909830633786186 1107 8 0.016042425972159435 1107 9 0.3104031682358013 1107 10-11 0.2111947164842931 1107 12-13 0.15839099534584022 1107 14-15 0.31486791652320534 1107 16-17 0.22533946788693981 1107 18-19 0.08305950837699072 1107 20-21 0.20688803341455753 1107 22-23 0.07804023255008019 1107 24-25 0.17332612278941895 1107 26-27 0.05973153066311454 1107 28-29 0.12454959283255107 1107 30-31 0.06166047940922681 1107 32-33 -0.01583522569453777 1107 34-35 0.1495426029728435 1107 36-37 0.13164892232077818 1107 38-39 0.14347487981835627 1107 40-41 0.14437393229059836 1107 42-43 0.13730588826763324 1107 44-45 0.16265607176496388 1107 46-47 0.2563939310403782 1107 48-49 0.2917357618551648 1107 50-51 0.08719369819753808 1107 52-53 0.10603057637010238 1107 54-55 0.1799882846778189 1107 56-57 0.25302364598787364 1107 58-59 0.2849650579383862 1107 60-61 0.2819244241860872 1107 62-63 0.28070793584323894 1107 64-65 0.09720316848162724 1107 66-67 0.1195501042756355 1107 68-69 0.1122982320760535 1107 70-71 0.10433722619109176 1107 72-73 0.2398377401531313 1107 74-75 0.2743505059542919 1107 76-77 0.1925159194876187 1107 78-79 0.2020890542976339 1107 80-81 0.19853529049428786 1107 82-83 0.10803122083472516 1107 84-85 0.13900208485213383 1107 86-87 0.2280932447522872 1107 88-89 0.14049512318736745 1107 90-91 0.10770640000236753 1107 92-93 0.11502748137996832 1107 94-95 0.30385463217205455 1107 96-97 0.23047875605391965 1107 98-99 0.17245346803578698 1108 1 0.13865937842849974 1108 2 0.2812103272278321 1108 3 0.23509423688714293 1108 4 0.12447812586792395 1108 5 -0.0027979968816893575 1108 6 0.0952012151314463 1108 7 0.11161648189054318 1108 8 0.054200782196119235 1108 9 0.30835289393852605 1108 10-11 0.24994577689448505 1108 12-13 0.20994814562022412 1108 14-15 0.2860944388490836 1108 16-17 0.3109941572771504 1108 18-19 0.09796647894334853 1108 20-21 0.2751582218343529 1108 22-23 0.18033451916937793 1108 24-25 0.19664904766128188 1108 26-27 0.03632676921247935 1108 28-29 0.14423306847001527 1108 30-31 0.05774733298794388 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2216 7 -0.38179134036144546 2216 8 -0.32729243197528746 2216 9 0.06655016038085648 2216 10-11 -0.301131406875065 2216 12-13 -0.19349999449014632 2216 14-15 -0.6211178763130434 2216 16-17 -0.7579355864990163 2216 18-19 -0.7035569936491726 2216 20-21 -1.0822304520098456 2216 22-23 -0.93360582382806 2216 24-25 -0.7158983169530728 2216 26-27 -0.8806296807102569 2216 28-29 -0.9551210447030485 2216 30-31 -0.6913190238210944 2216 32-33 -0.7018092975825141 2216 34-35 -0.7130297290224448 2216 36-37 -0.7441486404855624 2216 38-39 -0.8017922318116533 2216 40-41 -0.7463697103357774 2216 42-43 -0.8425759325034079 2216 44-45 -0.74156326344729 2216 46-47 -0.9382513900105209 2216 48-49 -1.1243746090078517 2216 50-51 -0.7705988845420535 2216 52-53 -0.6494637536688899 2216 54-55 -0.697753069500223 2216 56-57 -0.7612577300743624 2216 58-59 -0.7164244585705859 2216 60-61 -0.8012166754144872 2216 62-63 -0.717174466775532 2216 64-65 -0.8384900435239828 2216 66-67 -0.8564553125587011 2216 68-69 -0.8593166931580392 2216 70-71 -0.8361091014105568 2216 72-73 -0.7539454077957792 2216 74-75 -0.7852442241241064 2216 76-77 -0.8676968519935997 2216 78-79 -0.8106491119351915 2216 80-81 -0.7336801748960795 2216 82-83 -0.6919046384711329 2216 84-85 -0.7581083310620897 2216 86-87 -0.9058368235651884 2216 88-89 -0.7155510382593384 2216 90-91 -0.7749226583282471 2216 92-93 -0.730984097542482 2216 94-95 -0.8380871280776638 2216 96-97 -0.8695709682556299 2216 98-99 -0.7315109995468241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 31.0 10 114.0 11 300.0 12 540.0 13 1062.0 14 2831.0 15 7857.0 16 18241.0 17 33699.0 18 52383.0 19 67826.0 20 82894.0 21 101007.0 22 109938.0 23 86866.0 24 57283.0 25 45276.0 26 49439.0 27 62613.0 28 80926.0 29 105729.0 30 138149.0 31 180447.0 32 236328.0 33 311726.0 34 411943.0 35 570397.0 36 903564.0 37 1221860.0 38 394184.0 39 435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.252051424371075 20.335953556052264 13.878973462744334 22.53302155683233 2 14.14076079683533 22.676587311256164 38.16101342615726 25.021638465751245 3 17.902517517221114 30.30066950380555 30.555341075119212 21.241471903854126 4 12.363143781770695 19.25344441004428 36.90005344935767 31.483358358827353 5 11.183046833622514 39.4036900308146 34.15298825926546 15.26027487629742 6 27.672111804361858 38.13647277718514 18.346435797426185 15.84497962102681 7 21.78177519335099 34.706455827816605 24.466612142824477 19.045156836007926 8 27.563695067291466 31.806059792814395 20.75806631768409 19.872178822210046 9 27.331801136234784 13.04450986966454 21.6970878946746 37.92660109942607 10-11 24.25501678069946 26.70635949903034 26.446384222169417 22.592239498100785 12-13 27.28212077755697 24.842800416500186 27.20543256872515 20.669646237217695 14-15 24.95438784695457 22.95237275966317 28.22260754769387 23.870631845688393 16-17 20.538676492361784 25.74531408426683 30.531976302882303 23.184033120489087 18-19 21.447220071170854 24.301222738391257 34.681970699556885 19.569586490881 20-21 22.10219022423992 23.483552515680604 34.19829336875634 20.21596389132314 22-23 21.809793001807382 23.730633978348887 32.009699596618525 22.44987342322521 24-25 21.41552902495028 24.659354049894564 32.09453077386124 21.830586151293918 26-27 22.160746506863333 22.9827365321487 32.42706186706927 22.429455093918694 28-29 20.954387757473352 23.011906912658652 34.180517521718954 21.85318780814904 30-31 22.41939117208519 23.153373419102184 33.38505539467441 21.042180014138218 32-33 20.76702451998654 24.03039641731879 32.9344184627425 22.26816059995217 34-35 20.384070742061496 24.558274417848036 33.89484832151775 21.162806518572715 36-37 22.440793404364257 23.32416960463218 32.41103830437348 21.823998686630087 38-39 22.891870750007683 22.77234059459974 32.18207939740909 22.153709257983483 40-41 21.424712119360738 23.253872079869684 32.595018789735626 22.726397011033956 42-43 23.18246883557613 23.073405533695208 31.079780100496784 22.664345530231873 44-45 23.305368761184354 23.3429634612687 30.694179765074352 22.657488012472594 46-47 22.426053600785025 23.61407427286759 31.164049047469476 22.79582307887791 48-49 23.833231632124487 24.303799627128885 29.50888061452518 22.354088126221445 50-51 22.042443887544948 25.396953311648733 28.089717707929623 24.470885092876692 52-53 21.115398746843425 26.133609875999024 26.537947637744473 26.213043739413074 54-55 21.64284809362708 26.10259915305632 26.144541531200403 26.110011222116192 56-57 23.252694633222994 24.18514431895649 26.354775609264713 26.207385438555804 58-59 21.736272023496728 24.360791035500718 28.707824296293854 25.1951126447087 60-61 22.587254389706537 25.097809325975863 27.51042374920632 24.804512535111282 62-63 21.4119307512221 24.800773703815597 27.597653775601156 26.18964176936115 64-65 20.216338711503155 25.40048599184541 28.224924717366846 26.158250579284587 66-67 23.0209025969791 24.27936135139167 27.405436616783096 25.294299434846135 68-69 22.48171804935178 23.729704408649095 27.78252090058549 26.00605664141364 70-71 21.600964414943935 24.398367466269605 27.701863470441367 26.298804648345094 72-73 23.316954316166818 23.556586227757233 27.910225694223197 25.216233761852752 74-75 23.34757712487434 23.773082363736 27.808265234753627 25.071075276636034 76-77 21.697084573675777 24.470435308660672 28.40325478851521 25.42922532914834 78-79 22.093144855160208 24.845352980521792 27.86098625882496 25.20051590549304 80-81 20.949297580137337 25.319162493627996 27.96924195628055 25.762297969954123 82-83 21.249708577310038 24.6802502749306 27.112898836783057 26.957142310976305 84-85 21.82176850655898 25.21531545241171 26.025892203215506 26.93702383781381 86-87 21.409241416430465 24.14097774092879 27.07038485786444 27.379395984776306 88-89 21.87678273848121 23.10821695791444 27.47017743237682 27.54482287122753 90-91 21.992868636506238 23.77047137240703 27.046640601544343 27.190019389542393 92-93 21.36311902049335 23.887656444762378 27.11524301097355 27.63398152377072 94-95 20.06002778369945 24.262741028277997 27.92526981345565 27.751961374566903 96-97 21.71477314298394 23.66795905149149 26.758100644093414 27.85916716143116 98-99 21.93503916496061 23.743462199010025 26.432778249634737 27.888720386394628 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 234.0 1 204.5 2 408.0 3 638.0 4 412.0 5 327.5 6 757.5 7 1962.5 8 3439.5 9 3313.0 10 1814.5 11 976.5 12 933.0 13 1026.5 14 1383.5 15 1965.0 16 2569.5 17 3331.5 18 4433.5 19 5718.5 20 7250.5 21 9259.5 22 11661.5 23 14418.5 24 17466.0 25 21261.5 26 25633.5 27 30048.5 28 36775.5 29 46663.0 30 55073.5 31 64287.5 32 74852.0 33 82398.5 34 89002.0 35 97763.5 36 99834.5 37 107344.5 38 124124.0 39 117009.0 40 105159.0 41 109038.0 42 112584.0 43 122569.5 44 148630.5 45 189943.0 46 217566.5 47 255114.0 48 299173.5 49 354686.5 50 433439.0 51 378237.5 52 267604.5 53 224767.0 54 176591.0 55 151482.0 56 132563.0 57 96594.0 58 85448.5 59 71281.5 60 47469.0 61 36759.5 62 27853.0 63 20787.0 64 13530.0 65 9438.0 66 9721.0 67 8307.0 68 6894.5 69 5348.5 70 3869.0 71 3570.0 72 3432.0 73 2797.5 74 2267.5 75 2662.0 76 3776.5 77 3921.5 78 2677.0 79 2411.0 80 3137.5 81 3072.0 82 3450.0 83 3169.5 84 2048.5 85 1534.0 86 736.5 87 253.0 88 197.0 89 187.0 90 132.0 91 67.5 92 38.5 93 26.0 94 14.5 95 4.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05644791911080659 2 0.009108130374452855 3 0.0 4 0.0 5 0.002061510990102498 6 0.0 7 0.0 8 0.0 9 0.0037294607911854292 10-11 0.0 12-13 0.0 14-15 0.0026799642871332477 16-17 0.00416050399820686 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.278238015312154E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.2181655850605671E-4 54-55 0.0 56-57 0.0010213849905507833 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.001808507368589919 70-71 2.717446305135111E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.809712790288859E-4 80-81 9.745324680484537E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 4.8726623402422684E-4 92-93 0.0011806835670587035 94-95 5.528597655274882E-4 96-97 3.7482018001863606E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5335892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.491088555536518 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59686754282204 11.97201371503233 2 12.4424168880353 4.103779974909865 3 4.948187573183494 2.44802998376323 4 2.5519637536824535 1.6833864100998692 5 1.4714974008229997 1.213329697310695 6 0.9562462060139727 0.9461724518563346 7 0.6614265880913895 0.7635351106041038 8 0.4760026150590178 0.6279841022084178 9 0.37787278870323976 0.56083802591094 >10 2.5095702182621715 8.31824352543856 >50 0.3816594745550387 4.445761257082176 >100 0.45943129715596365 16.641285928415083 >500 0.08907613748358616 10.303686817064923 >1k 0.07102803119606216 21.50288388010982 >5k 0.004657575816135223 5.315359093832283 >10k+ 0.0020959091172608507 9.153710026361418 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 82202 1.5405484218945962 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 76046 1.4251787704848597 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31156 0.5838948764330313 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27351 0.5125853371844857 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24383 0.4569620224697202 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 23781 0.4456799350511592 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 23659 0.4433935319530455 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 23133 0.43353576121855536 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 22505 0.42176640756597017 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22421 0.4201921628098919 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20668 0.3873391740312585 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 19716 0.36949773346237147 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16417 0.30767114476829743 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14303 0.2680526517403276 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13555 0.2540343770076306 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 13084 0.24520736176819172 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 11572 0.21687095615878282 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 10751 0.20148458776901781 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 9521 0.1784331466978717 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 9459 0.17727120413981393 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 9451 0.17712127606780648 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8901 0.16681372111729398 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 8484 0.15899872036390542 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8474 0.15881131027389608 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 8097 0.1517459498805448 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7892 0.1479040430353538 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 7752 0.14528030177522333 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7721 0.14469933049619446 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7543 0.14136343089402859 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 7496 0.1404826034709848 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 7383 0.13836486945387952 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7373 0.1381774593638702 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 7137 0.13375458123965028 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 6991 0.13101839392551423 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 6888 0.12908806999841824 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 6877 0.128881918899408 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 6766 0.12680166690030456 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 6765 0.12678292589130363 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6720 0.1259395804862617 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 6701 0.125583501315244 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 6576 0.12324087519012754 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 6501 0.12183529951505766 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6464 0.12114188218202318 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 6417 0.12026105475897939 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 6368 0.11934274531793373 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 6280 0.11769353652585172 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6210 0.11638166589578648 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 6113 0.11456378802269611 No Hit CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA 6041 0.11321443537462901 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5840 0.10944749256544173 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5725 0.10729227653033457 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5714 0.10708612543132431 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 5623 0.10538069361223953 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 5470 0.10251331923509698 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 5358 0.10041432622699259 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.748201800186361E-5 0.0 2 0.0 0.0 0.0 3.748201800186361E-5 0.0 3 0.0 0.0 0.0 5.6223027002795406E-5 0.0 4 0.0 0.0 0.0 5.6223027002795406E-5 0.0 5 0.0 0.0 0.0 1.6866908100838623E-4 0.0 6 1.8741009000931804E-5 0.0 0.0 1.6866908100838623E-4 0.0 7 1.8741009000931804E-5 0.0 0.0 1.6866908100838623E-4 0.0 8 1.8741009000931804E-5 0.0 0.0 1.6866908100838623E-4 0.0 9 3.748201800186361E-5 0.0 0.0 1.6866908100838623E-4 0.0 10-11 5.6223027002795406E-5 0.0 0.0 2.1552160351071573E-4 0.0 12-13 8.433454050419312E-5 0.0 0.0 2.3426261251164752E-4 0.0 14-15 1.0307554950512491E-4 0.0 0.0 3.3733816201677246E-4 0.0 16-17 1.311870630065226E-4 0.0 0.0 5.060072430251586E-4 0.0 18-19 1.6866908100838623E-4 0.0 0.0 5.622302700279541E-4 0.0 20-21 2.2489210801118162E-4 0.0 0.0 0.0011150900355554423 0.0 22-23 2.3426261251164752E-4 0.0 0.0 0.004685252250232951 0.0 24-25 2.623741260130452E-4 0.0 0.0 0.013081224282650399 0.0 26-27 2.8111513501397707E-4 0.0 0.0 0.022826548963134935 0.0 28-29 2.8111513501397707E-4 0.0 0.0 0.05121917759954662 0.0 30-31 3.3733816201677246E-4 0.0 0.0 0.13147754864603706 0.0 32-33 3.3733816201677246E-4 0.0 0.0 0.26251468358055224 0.0 34-35 3.5607917101770426E-4 0.0 0.0 0.42345309837605405 0.0 36-37 3.5607917101770426E-4 0.0 0.0 0.6637128337679998 0.0 38-39 3.5607917101770426E-4 0.0 0.0 1.0263326169270293 0.0 40-41 3.6544967551817016E-4 0.0 0.0 1.5113593003756447 0.0 42-43 3.7482018001863606E-4 0.0 0.0 1.8887188871139071 0.0 44-45 3.8419068451910196E-4 0.0 0.0 2.2895609581303367 0.0 46-47 4.0293169352003375E-4 0.0 0.0 2.710699541894776 0.0 48-49 4.1230219802049965E-4 0.0 0.0 3.1450599075093724 0.0 50-51 4.1230219802049965E-4 0.0 0.0 3.6540188594521776 0.0 52-53 4.1230219802049965E-4 0.0 0.0 4.182449719746951 0.0 54-55 4.1230219802049965E-4 0.0 0.0 4.646533325637026 0.0 56-57 4.1230219802049965E-4 0.0 0.0 5.1449785715303085 0.0 58-59 4.1230219802049965E-4 0.0 0.0 5.709485874151875 0.0 60-61 4.1230219802049965E-4 0.0 0.0 6.2458348107495425 0.0 62-63 4.1230219802049965E-4 0.0 0.0 6.786840888083942 0.0 64-65 4.3104320702143145E-4 0.0 0.0 7.364926051726684 0.0 66-67 4.4978421602236325E-4 0.0 0.0 7.9698483402587605 0.0 68-69 4.4978421602236325E-4 0.0 0.0 8.65950810098855 0.0 70-71 4.4978421602236325E-4 0.0 0.0 9.504680754408072 0.0 72-73 4.4978421602236325E-4 0.0 0.0 10.211704059977226 0.0 74-75 4.4978421602236325E-4 0.0 0.0 10.883990905363152 0.0 76-77 4.4978421602236325E-4 0.0 0.0 11.612519518760873 0.0 78-79 4.5915472052282915E-4 0.0 0.0 12.296163415601365 0.0 80-81 4.685252250232951E-4 0.0 0.0 13.055193021148103 0.0 82-83 4.77895729523761E-4 0.0 0.0 13.928702080177036 0.0 84-85 4.872662340242269E-4 0.0 0.0 14.735436924135646 0.0 86-87 4.872662340242269E-4 0.0 0.0 15.50965611747764 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 545 0.0 58.876575 2 TCACGCA 600 0.0 52.70202 4 CCGAGTA 515 0.0 35.21527 8 CGCCGAG 590 0.0 33.102654 6 TAACGCA 560 0.0 31.55478 4 TAATAAT 2650 0.0 30.182287 3 TTCTGGC 2710 0.0 29.343277 9 TATTCGC 635 0.0 28.560953 9 ATAACGC 570 0.0 27.737904 3 ATCACGC 1265 0.0 27.202623 3 GCCGAGT 655 0.0 26.97788 7 TTAAGGT 970 0.0 26.84639 4 TCAAGAC 990 0.0 26.77375 3 AGGGCTA 2740 0.0 26.136038 5 TTCAACG 730 0.0 26.118662 2 CTAACTG 1565 0.0 25.553694 4 TATTAAG 1035 0.0 25.161562 2 CGCAGCG 1045 0.0 24.47485 8 TAGGCAT 5750 0.0 24.423546 5 AACGCCG 805 0.0 24.261799 4 >>END_MODULE