FastQCFastQC Report
Fri 27 May 2016
ERR765258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5220357
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA134550.2577409935757267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT130840.2506341999215762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC2705.4569682E-1122.3885429
CTAACGC10350.020.6663463
TCTAACG10400.019.2256642
GTACTAG18950.019.139911
GTACATA13250.018.9510121
CATGGGG125850.018.7327234
TAGTACT21100.018.7319364
ACGTTAA4350.018.1721328
TACATGA20300.018.0957782
GTATAAG19950.017.9474331
CGGGCGT12700.017.9406666
TACGGGC13000.017.8843364
GTATAAT15100.017.860991
CGTTAAC4450.017.763779
GTACATG385150.017.433791
GGCGTCG13800.017.1845158
TACATGG375950.016.9571762
GTACAAG24700.016.7551211
GTATCAA265950.016.2256641
ATACGGG15800.016.1864573
Produced by FastQC (version 0.11.5)