##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765258_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5220357 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.855220246431422 33.0 33.0 33.0 27.0 33.0 2 30.635244103037397 33.0 33.0 33.0 27.0 33.0 3 30.775441986055743 33.0 33.0 33.0 27.0 33.0 4 34.25403703233323 37.0 33.0 37.0 27.0 37.0 5 34.39805534372458 37.0 33.0 37.0 27.0 37.0 6 34.32430751383478 37.0 33.0 37.0 27.0 37.0 7 34.21340532840953 37.0 33.0 37.0 27.0 37.0 8 34.12506807484622 37.0 33.0 37.0 27.0 37.0 9 34.059366246408054 37.0 37.0 37.0 27.0 37.0 10-11 34.03295742034501 37.0 33.0 37.0 27.0 37.0 12-13 33.960100429913126 37.0 33.0 37.0 27.0 37.0 14-15 35.17426787095212 37.0 33.0 40.0 27.0 40.0 16-17 35.23428675471811 37.0 33.0 40.0 27.0 40.0 18-19 35.25535447096817 37.0 33.0 40.0 27.0 40.0 20-21 35.26899328915628 37.0 33.0 40.0 27.0 40.0 22-23 35.33144466939713 37.0 33.0 40.0 27.0 40.0 24-25 35.28511268482213 37.0 33.0 40.0 27.0 40.0 26-27 35.294877342679825 37.0 33.0 40.0 27.0 40.0 28-29 35.22087301692202 37.0 33.0 40.0 27.0 40.0 30-31 35.17804433681451 37.0 33.0 40.0 27.0 40.0 32-33 35.03392612804066 37.0 33.0 40.0 27.0 40.0 34-35 34.97267476151535 37.0 33.0 40.0 27.0 40.0 36-37 34.84820003689403 37.0 33.0 40.0 27.0 40.0 38-39 34.615406570853295 37.0 33.0 40.0 27.0 40.0 40-41 34.53258273332648 37.0 33.0 40.0 27.0 40.0 42-43 34.39487088718262 37.0 33.0 40.0 22.0 40.0 44-45 34.24352980073968 37.0 33.0 40.0 22.0 40.0 46-47 34.111048918685064 37.0 33.0 40.0 22.0 40.0 48-49 33.93314748397475 37.0 33.0 40.0 22.0 40.0 50-51 33.75836642972885 37.0 33.0 40.0 22.0 40.0 52-53 33.56082218131825 37.0 33.0 37.0 22.0 40.0 54-55 33.43620321368826 37.0 33.0 37.0 22.0 40.0 56-57 33.23871681572735 37.0 33.0 37.0 22.0 40.0 58-59 32.98796748957973 37.0 33.0 37.0 22.0 40.0 60-61 32.74686606682263 37.0 33.0 37.0 22.0 40.0 62-63 32.57119886628443 37.0 33.0 37.0 22.0 40.0 64-65 32.24517786810365 37.0 33.0 37.0 22.0 40.0 66-67 32.02527106862615 33.0 27.0 37.0 22.0 40.0 68-69 31.712838125821662 33.0 27.0 37.0 22.0 37.0 70-71 31.572342562012523 33.0 27.0 37.0 22.0 37.0 72-73 31.286611049780696 33.0 27.0 37.0 22.0 37.0 74-75 30.98574676023115 33.0 27.0 37.0 15.0 37.0 76-77 30.783215592343588 33.0 27.0 37.0 15.0 37.0 78-79 30.53200317526177 33.0 27.0 37.0 15.0 37.0 80-81 30.26033267456613 33.0 27.0 37.0 15.0 37.0 82-83 30.069764768961203 33.0 27.0 37.0 15.0 37.0 84-85 29.87658353633669 33.0 27.0 37.0 15.0 37.0 86-87 29.65160562773772 33.0 27.0 37.0 15.0 37.0 88-89 29.459760893747305 33.0 27.0 37.0 15.0 37.0 90-91 29.244058212877015 33.0 27.0 37.0 15.0 37.0 92-93 29.08133294332169 33.0 27.0 35.0 15.0 37.0 94-95 28.820142089899214 33.0 27.0 33.0 15.0 37.0 96-97 28.727803098523722 33.0 27.0 33.0 15.0 37.0 98-99 27.586205215467064 33.0 24.5 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4479027002331435 1101 2 0.46614376433580773 1101 3 0.5819669388594733 1101 4 0.5859866150655293 1101 5 0.5205340033498302 1101 6 0.49547488647229443 1101 7 0.4056105130289538 1101 8 0.47631355561723154 1101 9 0.44382202879198474 1101 10-11 0.48865750306147504 1101 12-13 0.49442576925852677 1101 14-15 0.6381152284317935 1101 16-17 0.6157480918368776 1101 18-19 0.6001131060449012 1101 20-21 0.6387637535476856 1101 22-23 0.5321235281775358 1101 24-25 0.5615039735471257 1101 26-27 0.6721205221019915 1101 28-29 0.6337021563820571 1101 30-31 0.6446404545587043 1101 32-33 0.76925297275384 1101 34-35 0.6235338801570194 1101 36-37 0.6979350322843416 1101 38-39 0.7055538238575707 1101 40-41 0.4673710966104565 1101 42-43 0.5656417634406878 1101 44-45 0.5742451913699966 1101 46-47 0.5911069952795813 1101 48-49 0.7249827935610327 1101 50-51 0.7546511688746804 1101 52-53 0.6456049487636477 1101 54-55 0.7408508917795515 1101 56-57 0.7607934528436644 1101 58-59 0.7125735587041788 1101 60-61 0.8358374373853437 1101 62-63 0.7080640643475249 1101 64-65 0.551269103329382 1101 66-67 0.6750507673010446 1101 68-69 0.6478015745260599 1101 70-71 0.6673368190301723 1101 72-73 0.7313899078683015 1101 74-75 0.614906836202394 1101 76-77 0.544043943395053 1101 78-79 0.5947367241009225 1101 80-81 0.5164381035004411 1101 82-83 0.5840346504095031 1101 84-85 0.525843273605151 1101 86-87 0.5994504985072702 1101 88-89 0.5781511847509044 1101 90-91 0.5317239523053452 1101 92-93 0.6636840830438864 1101 94-95 0.714176454564182 1101 96-97 0.6905970449126997 1101 98-99 0.6477170353939918 1102 1 0.42980890042328923 1102 2 0.4183774148221211 1102 3 0.39456712266581917 1102 4 0.5035066716275622 1102 5 0.4910572509661151 1102 6 0.4421196102088132 1102 7 0.35464210372008864 1102 8 0.38506403808816714 1102 9 0.39043032833278346 1102 10-11 0.4046611895032086 1102 12-13 0.46710753036977337 1102 14-15 0.4811007894385497 1102 16-17 0.6110714046943855 1102 18-19 0.5552764407035014 1102 20-21 0.5153572200139891 1102 22-23 0.40439110335850614 1102 24-25 0.6001538672170241 1102 26-27 0.6274308428955493 1102 28-29 0.39487409267664475 1102 30-31 0.4145925330264575 1102 32-33 0.6309342767897803 1102 34-35 0.5726360628734923 1102 36-37 0.7061456031845665 1102 38-39 0.543202802732182 1102 40-41 0.5702073303872908 1102 42-43 0.5715557911030018 1102 44-45 0.4952199286345831 1102 46-47 0.5600477244100119 1102 48-49 0.5489324988842199 1102 50-51 0.5848074236090213 1102 52-53 0.5331701335687811 1102 54-55 0.5543636145070607 1102 56-57 0.5771935874151879 1102 58-59 0.5569885967119745 1102 60-61 0.7166703164523156 1102 62-63 0.6713513991251148 1102 64-65 0.5769905257388857 1102 66-67 0.5900333329587681 1102 68-69 0.5626781370250633 1102 70-71 0.611308831064985 1102 72-73 0.5642426352209107 1102 74-75 0.6453985619236917 1102 76-77 0.562952682779887 1102 78-79 0.6722879120684659 1102 80-81 0.5848028652507438 1102 82-83 0.5468785862229808 1102 84-85 0.5316400853506345 1102 86-87 0.6474435207181095 1102 88-89 0.6653234026768224 1102 90-91 0.6091011468170926 1102 92-93 0.7056965838647002 1102 94-95 0.7124525678344966 1102 96-97 0.6641530097454371 1102 98-99 0.6223708144342304 1103 1 0.23163094923865657 1103 2 0.26446034731930723 1103 3 0.28952713633407257 1103 4 0.3565983541179065 1103 5 0.3126291366805418 1103 6 0.302751298791506 1103 7 0.23587722626923835 1103 8 0.22410921811329843 1103 9 0.2869984291325096 1103 10-11 0.22080154022039267 1103 12-13 0.2272349740718198 1103 14-15 0.2923637075500167 1103 16-17 0.19560674485333607 1103 18-19 0.31323043134123907 1103 20-21 0.2730397760261596 1103 22-23 0.3512159399383563 1103 24-25 0.4600508719358558 1103 26-27 0.41211773164702237 1103 28-29 0.3238597294573822 1103 30-31 0.38789810727858054 1103 32-33 0.457023861008544 1103 34-35 0.38606671867608355 1103 36-37 0.3943827267223625 1103 38-39 0.30906642906352033 1103 40-41 0.30544979597497246 1103 42-43 0.3522509628894852 1103 44-45 0.27410542478065736 1103 46-47 0.2506123120792765 1103 48-49 0.3502212395382145 1103 50-51 0.4153186850222852 1103 52-53 0.32069178662495545 1103 54-55 0.33951402610512815 1103 56-57 0.2965488355566279 1103 58-59 0.38569234095024285 1103 60-61 0.37760013166943196 1103 62-63 0.3210877623358712 1103 64-65 0.23173337346614886 1103 66-67 0.3871558249587679 1103 68-69 0.3764757167469135 1103 70-71 0.3307734769216104 1103 72-73 0.29263738449538934 1103 74-75 0.337785016002794 1103 76-77 0.29114125300631954 1103 78-79 0.36416007376245574 1103 80-81 0.29394422467050063 1103 82-83 0.3208263343355391 1103 84-85 0.33853858640280876 1103 86-87 0.4444428281851245 1103 88-89 0.4485094587470648 1103 90-91 0.38802520653179684 1103 92-93 0.4994776802070433 1103 94-95 0.4749102573523203 1103 96-97 0.21611222751756998 1103 98-99 0.43552559707676153 1104 1 0.21949548834500732 1104 2 0.13448427979226807 1104 3 0.13237740491027594 1104 4 0.27116844244309135 1104 5 0.24742087437602578 1104 6 0.13636998706854087 1104 7 0.0018420953540925211 1104 8 0.08585080873169915 1104 9 0.12601431054305579 1104 10-11 0.0984380741741191 1104 12-13 0.0679893296877907 1104 14-15 0.22641492146345854 1104 16-17 0.18010998285650714 1104 18-19 0.15216668710993986 1104 20-21 0.2302308572256493 1104 22-23 0.21188692960971167 1104 24-25 0.18526930926044116 1104 26-27 0.2289284419675539 1104 28-29 0.1379052646075749 1104 30-31 0.22448950895400088 1104 32-33 0.24586983491568049 1104 34-35 0.232599933141465 1104 36-37 0.2519967475144398 1104 38-39 0.19293933345415581 1104 40-41 0.196233888819485 1104 42-43 0.06702749649711137 1104 44-45 0.10560780603591269 1104 46-47 0.16442624901813474 1104 48-49 0.1994561396596879 1104 50-51 0.17510465439369938 1104 52-53 0.020694875714291072 1104 54-55 0.22539023149511905 1104 56-57 0.13385934990593995 1104 58-59 0.1911970298387189 1104 60-61 0.2041864663275632 1104 62-63 0.07075977239340148 1104 64-65 0.15625799525383144 1104 66-67 0.03878927771664564 1104 68-69 0.030369320313074155 1104 70-71 0.0500327269605485 1104 72-73 0.053015320534392174 1104 74-75 -0.019177927979797005 1104 76-77 -0.012570215727389922 1104 78-79 0.06632944936566076 1104 80-81 0.13575796336116852 1104 82-83 0.116561584654999 1104 84-85 0.10122702464728306 1104 86-87 0.13882989973146564 1104 88-89 0.13008217560645008 1104 90-91 0.2129805323798699 1104 92-93 0.12413750847492722 1104 94-95 0.05154714833494012 1104 96-97 -0.18433050653445093 1104 98-99 -0.049538329615732124 1105 1 0.0704197858987925 1105 2 0.14010776776731504 1105 3 0.04077430404808524 1105 4 0.027053365483119762 1105 5 0.07087879145576892 1105 6 0.04604428927069648 1105 7 -0.014203271070861945 1105 8 -0.05710297924765939 1105 9 -0.058204284090756175 1105 10-11 -0.0318865990165591 1105 12-13 -0.020679143863091554 1105 14-15 -0.001219996157246328 1105 16-17 -0.07225835927920343 1105 18-19 0.0013050269458005914 1105 20-21 0.09324782467805903 1105 22-23 -0.027450852173508622 1105 24-25 0.10403216276692007 1105 26-27 0.09907203753331117 1105 28-29 0.03383818334334876 1105 30-31 0.10416982698190935 1105 32-33 0.09713486197102128 1105 34-35 0.01557108290641196 1105 36-37 0.10979514182892558 1105 38-39 0.02735134876757428 1105 40-41 -0.07224734585845738 1105 42-43 -0.06002626417853918 1105 44-45 -0.13947593133801206 1105 46-47 -0.17664893274987037 1105 48-49 -0.1559156907056405 1105 50-51 0.02361945470671145 1105 52-53 -0.04354237045848208 1105 54-55 0.018394731331575542 1105 56-57 0.05071087568024524 1105 58-59 -0.05794129034548945 1105 60-61 0.006236729764069082 1105 62-63 0.00856140832431862 1105 64-65 -0.18875151629938358 1105 66-67 -0.07429111923836018 1105 68-69 -0.19073315972303462 1105 70-71 -0.24524288465873312 1105 72-73 -0.10199248404565964 1105 74-75 -0.14942107702664487 1105 76-77 -0.06636007408761557 1105 78-79 -0.010231041110674965 1105 80-81 -0.020442991582388714 1105 82-83 0.008179324490097173 1105 84-85 -0.0350639521189855 1105 86-87 -0.03519014241921781 1105 88-89 -0.017401946685993153 1105 90-91 3.029596279979785E-4 1105 92-93 0.13246773256337718 1105 94-95 0.05864342219632235 1105 96-97 -0.10086681972817502 1105 98-99 -0.05794576204177204 1106 1 0.11661266270550996 1106 2 0.18296438365373646 1106 3 0.09656286586315943 1106 4 0.09409284085133862 1106 5 0.06224438042480074 1106 6 0.08173816964839631 1106 7 0.09000580959569504 1106 8 0.09265258192122161 1106 9 0.03809345440004108 1106 10-11 -0.05068792624973639 1106 12-13 0.0761537950430693 1106 14-15 0.06526302684338958 1106 16-17 0.023406682879169693 1106 18-19 0.12715394489630683 1106 20-21 0.10457595759859117 1106 22-23 0.10294026414395319 1106 24-25 0.1423577087425727 1106 26-27 0.10525084312394029 1106 28-29 -0.0031650714862436757 1106 30-31 0.036254115002790854 1106 32-33 0.14270699851578428 1106 34-35 -0.06191916558064037 1106 36-37 -0.05057133582049289 1106 38-39 -0.009765880370380842 1106 40-41 0.14516346525496004 1106 42-43 0.07225416251336725 1106 44-45 0.020301107603543755 1106 46-47 -0.0699887541849904 1106 48-49 -0.07665983062021553 1106 50-51 0.03511377552889883 1106 52-53 -0.04742249218634953 1106 54-55 -0.12833632825766728 1106 56-57 -0.04628464509396224 1106 58-59 -0.050254320310507694 1106 60-61 0.10949980298079964 1106 62-63 0.038878086649503985 1106 64-65 -0.08556762803393525 1106 66-67 -0.022337411606230262 1106 68-69 0.03156994537339486 1106 70-71 0.015755754329592975 1106 72-73 0.13849661707243044 1106 74-75 0.030726194568522658 1106 76-77 -0.02801720732754731 1106 78-79 -0.04099132068587252 1106 80-81 -0.17758041391743973 1106 82-83 -0.015250790253301005 1106 84-85 -0.09921330977503473 1106 86-87 -0.15748484131925622 1106 88-89 -0.09343608175488782 1106 90-91 -0.1349533019685758 1106 92-93 -0.060488584240982135 1106 94-95 -0.1527562195531047 1106 96-97 -0.23029131213312581 1106 98-99 0.12602125977585743 1107 1 -0.0010775288881497147 1107 2 0.049549964281432324 1107 3 0.002469633340314914 1107 4 0.04897251023707838 1107 5 0.04446249122305801 1107 6 -0.04044348962240463 1107 7 -0.1441484531588273 1107 8 -0.09472551665768947 1107 9 -0.1768617729747035 1107 10-11 -0.036307696868917105 1107 12-13 -0.08587923575553447 1107 14-15 -0.0889300363931298 1107 16-17 -0.03524062732869737 1107 18-19 -0.10185606950223303 1107 20-21 -0.05838701476317709 1107 22-23 -0.058486772553202115 1107 24-25 0.07027002807134863 1107 26-27 0.10163248181914497 1107 28-29 -0.007937732618962912 1107 30-31 -0.022971355084905554 1107 32-33 -0.07628238982758972 1107 34-35 -0.06958724205755829 1107 36-37 -0.02140441137553495 1107 38-39 -0.045199879983670144 1107 40-41 -0.10110718657802664 1107 42-43 -0.15699167344478582 1107 44-45 -0.04927770119211061 1107 46-47 -0.2057670843399535 1107 48-49 -0.15921285047356548 1107 50-51 -0.1326931170731811 1107 52-53 -0.1931467984287707 1107 54-55 -0.13848152649200784 1107 56-57 -0.12686292281529177 1107 58-59 -0.05757333508435636 1107 60-61 0.01270500292968535 1107 62-63 -0.2660855754350777 1107 64-65 -0.3408221048662199 1107 66-67 -0.13884010940370217 1107 68-69 -0.11109537480824017 1107 70-71 -0.02545990241933893 1107 72-73 -0.049614818812589334 1107 74-75 -0.18158467966172154 1107 76-77 -0.19205560738177496 1107 78-79 -0.04652707118763644 1107 80-81 -0.2948487606364303 1107 82-83 -0.16696745365745258 1107 84-85 -0.3315343568773059 1107 86-87 -0.32197047029988823 1107 88-89 -0.13535989593902187 1107 90-91 -0.11606025343015958 1107 92-93 -0.09840466428885009 1107 94-95 -0.03091247954611731 1107 96-97 -0.06863151658872013 1107 98-99 0.24698638782042082 1108 1 -0.01079097693961728 1108 2 -0.04180509803243382 1108 3 0.011295691097551241 1108 4 -0.11229036451700836 1108 5 -0.03253082979922084 1108 6 -0.2557497990496884 1108 7 -0.10624753742912674 1108 8 -0.22535053236168068 1108 9 -0.13928395451027598 1108 10-11 -0.10659669491895585 1108 12-13 -0.19335361669443074 1108 14-15 -0.05669190254619849 1108 16-17 -0.09660533834859564 1108 18-19 -0.10568241485587748 1108 20-21 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2216 6 -0.030157740662033916 2216 7 -0.07067885384704908 2216 8 -0.0902877228543204 2216 9 -0.0227048009895654 2216 10-11 -0.15660674026379695 2216 12-13 -0.129898533781585 2216 14-15 -0.18752312240226132 2216 16-17 -0.12969861166929775 2216 18-19 -0.19887255693976158 2216 20-21 -0.2665594495678292 2216 22-23 -0.463489251926525 2216 24-25 -0.3269815257640758 2216 26-27 -0.36405738444955915 2216 28-29 -0.2534691165232985 2216 30-31 -0.3165156532215647 2216 32-33 -0.3311215845321911 2216 34-35 -0.4618441860410982 2216 36-37 -0.4401704783151388 2216 38-39 -0.31424534653883285 2216 40-41 -0.3535653769799936 2216 42-43 -0.3354029213612577 2216 44-45 -0.35115249615789423 2216 46-47 -0.15953279256844155 2216 48-49 -0.13157105370695632 2216 50-51 -0.16294681274582956 2216 52-53 0.056882886101398356 2216 54-55 -0.13971465521778725 2216 56-57 0.057007497455082046 2216 58-59 -0.25477712661346885 2216 60-61 -0.12159056902650178 2216 62-63 -0.09808682634133703 2216 64-65 0.020991706049088066 2216 66-67 -0.1468845933748426 2216 68-69 -0.2776947554863902 2216 70-71 -0.1886718965813543 2216 72-73 -0.16197351507624091 2216 74-75 -0.13379528038116817 2216 76-77 -0.17376895706096107 2216 78-79 -0.16014125636348098 2216 80-81 -0.054991127864330025 2216 82-83 -0.10352564734628444 2216 84-85 -0.03457669332241764 2216 86-87 -0.1572050357664061 2216 88-89 -0.1704083345817189 2216 90-91 -0.023116527777478524 2216 92-93 0.07066514563534554 2216 94-95 0.15817771686323567 2216 96-97 0.13180504432295237 2216 98-99 0.1178994988223927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 115.0 8 875.0 9 1777.0 10 3265.0 11 6973.0 12 14476.0 13 25683.0 14 33546.0 15 36416.0 16 38686.0 17 40609.0 18 42837.0 19 46187.0 20 50097.0 21 54365.0 22 59933.0 23 67206.0 24 75508.0 25 83657.0 26 91761.0 27 102772.0 28 120365.0 29 144374.0 30 176256.0 31 219230.0 32 278621.0 33 360309.0 34 475732.0 35 649418.0 36 880022.0 37 844752.0 38 194455.0 39 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.471243018504175 19.03903431037878 12.484550483235598 25.005172187881445 2 15.603798744032257 21.099246660716883 37.95840016305399 25.33855443219688 3 17.84490777633386 26.805797769958804 28.94651548401334 26.402778969693998 4 12.801078546926965 17.58960163069307 36.64843228154703 32.960887540832935 5 14.060341083952688 38.04933264142663 33.30398668137064 14.586339593250038 6 31.2014293275345 37.34629643145095 17.70179702269404 13.750477218320508 7 27.508999863419305 32.34905199012251 22.088508506219018 18.053439640239162 8 26.716525325758372 34.04328477918273 19.840616264366595 19.399573630692306 9 26.995375986738075 15.812864905599369 19.1253395122211 38.06641959544146 10-11 25.071082303375036 26.29790453028404 26.77474931312169 21.856263853219236 12-13 25.92905044616681 24.27069642938213 26.324310770317048 23.47594235413402 14-15 24.01551273217521 24.952019564945463 23.771650099792023 27.2608176030873 16-17 23.75622969846698 27.353962573823974 24.63612162922957 24.25368609847947 18-19 24.239003194609104 26.92150172871319 25.739120906865182 23.100374169812525 20-21 25.647999353298456 26.121235085014625 24.761160055375736 23.469605506311183 22-23 25.05589177138652 25.96105017338852 24.486696982600996 24.49636107262396 24-25 24.350847604224864 26.5342559345897 24.636340465418687 24.478555995766744 26-27 24.523629140163838 26.41624946962419 24.471123132430648 24.58899825778132 28-29 24.295327715489442 26.323286152269926 25.001946892974335 24.379439239266297 30-31 24.62869899037924 26.33094884007734 24.861816593272472 24.17853557627095 32-33 24.160822871714398 26.73743073264025 24.44040172003727 24.66134467560808 34-35 24.35869291622752 26.704242662470783 24.950673843191478 23.98639057811022 36-37 24.564823823351546 26.473151165715297 24.69428814925876 24.2677368616744 38-39 24.517722113422714 26.33562477964867 24.72546654620235 24.421186560726266 40-41 24.308969673913104 26.416383017483287 25.079252242710602 24.195395065893003 42-43 24.656915226295826 26.331819835310117 24.829796123138706 24.181468815255354 44-45 24.247201867611736 26.669651136885847 24.786360396424996 24.296786599077418 46-47 23.946772222666 26.60511532065719 25.200067734831162 24.248044721845652 48-49 24.498773740519546 26.433951612795088 25.048133773482874 24.019140873202492 50-51 23.76001296463058 26.738247978059736 24.884725316678534 24.61701374063115 52-53 23.702200826495197 26.66126090610278 24.623488393609865 25.013049873792152 54-55 23.976731859526083 26.443028704741838 24.688800018849285 24.89143941688279 56-57 24.17627759940556 25.975589408923565 24.70098309368497 25.147149897985905 58-59 23.86668191466599 25.939346676865206 25.401624831405208 24.792346577063597 60-61 24.02097212891762 26.196158615205817 24.941943625694567 24.840925630182 62-63 23.643756547684383 26.159810526368215 24.810056093864848 25.386376832082558 64-65 23.415659120631023 25.93770885784248 25.031286174489598 25.615345847036895 66-67 24.0806278345768 25.79933245931887 24.934295668724506 25.185744037379827 68-69 23.859441030565534 25.47881303903162 25.10277553814806 25.558970392254782 70-71 23.368085745859908 25.59078813958509 25.209482799739558 25.831643314815445 72-73 24.010139536434004 25.608957395059377 25.301114464010794 25.079788604495825 74-75 24.10557362264688 25.53659644349994 25.260513792447526 25.097316141405656 76-77 23.62784767401923 25.550091689131605 25.622787866806814 25.19927277004236 78-79 23.774782069502145 25.54822400077236 25.59870905380609 25.078284875919405 80-81 23.499063378232563 25.48069030527989 25.86095165522205 25.159294661265502 82-83 23.72470886569635 25.326103176468585 25.708529129329662 25.240658828505406 84-85 23.940642373692068 25.437130066008894 25.234260798638868 25.38796676166017 86-87 23.62783809613021 25.141738390688605 25.637097233005328 25.59332628017586 88-89 23.880416607523202 24.66811177856227 25.763534945981664 25.687936667932863 90-91 24.01478481260956 24.912721486289154 25.621303294008435 25.45119040709285 92-93 23.79558524445742 24.963867413665387 25.60776973682068 25.63277760505651 94-95 23.23640892758867 25.096185950501088 26.01220567865378 25.655199443256464 96-97 23.78004033057509 24.91604501377971 25.620929756336587 25.682984899308607 98-99 23.51656218147533 25.219922698773285 25.360641044281074 25.902874075470315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 557.0 1 568.5 2 563.0 3 535.0 4 502.5 5 451.5 6 420.0 7 410.5 8 396.5 9 400.5 10 421.5 11 405.0 12 396.5 13 416.5 14 444.5 15 570.0 16 681.5 17 747.5 18 956.5 19 1228.5 20 1710.5 21 2526.0 22 3791.5 23 5296.5 24 7049.5 25 9669.0 26 13690.5 27 17687.5 28 22406.5 29 28924.5 30 34634.5 31 42353.5 32 51562.0 33 59810.0 34 66932.0 35 75607.0 36 84339.5 37 94548.0 38 105513.5 39 105904.0 40 107632.0 41 116550.5 42 127718.5 43 143191.5 44 161309.0 45 181312.0 46 201981.5 47 222368.0 48 236187.0 49 246637.0 50 261060.5 51 254564.5 52 237762.0 53 233210.0 54 223165.0 55 210289.5 56 190543.5 57 171466.5 58 160104.5 59 141946.5 60 119244.5 61 94135.5 62 75711.0 63 61110.5 64 45011.0 65 33067.5 66 24184.5 67 18222.5 68 13767.0 69 11254.5 70 9736.5 71 8383.0 72 7973.5 73 7104.0 74 5304.5 75 3893.5 76 2812.0 77 1834.5 78 1189.5 79 814.0 80 555.0 81 369.5 82 261.5 83 186.5 84 130.5 85 106.5 86 62.5 87 40.5 88 34.0 89 22.0 90 18.0 91 18.5 92 14.0 93 7.5 94 5.0 95 3.5 96 0.5 97 2.0 98 2.5 99 7.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002471095367615663 2 0.0 3 9.577889021766135E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.831155608706454E-4 22-23 0.0 24-25 0.005009235958383689 26-27 3.831155608706454E-5 28-29 0.009606622688831433 30-31 5.842512303277342E-4 32-33 9.577889021766135E-5 34-35 5.746733413059682E-5 36-37 0.0 38-39 9.577889021766135E-6 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.915577804353227E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.915577804353227E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5220357.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.40781922449689 #Duplication Level Percentage of deduplicated Percentage of total 1 76.9794677089516 30.335929474723606 2 11.672702428404037 9.199914943197843 3 4.048463868630385 4.7862339681568065 4 1.9048735611207817 3.0026765176868557 5 1.1181487802822414 2.203190249972713 6 0.726034173882366 1.7166854085097933 7 0.49050710913284185 1.3530870839526319 8 0.370576186052168 1.168287947907789 9 0.3009210352658667 1.0672777582745157 >10 1.969687130511917 15.208434306891643 >50 0.21988593896465486 6.0226498961828785 >100 0.1732817874827886 13.775285467960218 >500 0.01780049354576076 4.814017729519229 >1k 0.0075517234422102135 4.833476940855551 >5k 0.0 0.0 >10k+ 9.807433041830668E-5 0.5128523062080065 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13455 0.2577409935757267 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 13084 0.2506341999215762 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.9155778043532273E-5 0.0 2 0.0 0.0 0.0 1.9155778043532273E-5 0.0 3 0.0 0.0 0.0 3.8311556087064545E-5 0.0 4 0.0 0.0 0.0 5.746733413059682E-5 0.0 5 0.0 0.0 0.0 5.746733413059682E-5 0.0 6 0.0 0.0 0.0 5.746733413059682E-5 0.0 7 0.0 0.0 0.0 7.662311217412909E-5 0.0 8 0.0 1.9155778043532273E-5 0.0 7.662311217412909E-5 1.9155778043532273E-5 9 0.0 1.9155778043532273E-5 0.0 7.662311217412909E-5 1.9155778043532273E-5 10-11 9.577889021766136E-6 1.9155778043532273E-5 0.0 1.1493466826119364E-4 1.9155778043532273E-5 12-13 3.8311556087064545E-5 1.9155778043532273E-5 0.0 1.1493466826119364E-4 1.9155778043532273E-5 14-15 7.662311217412909E-5 1.9155778043532273E-5 0.0 1.1493466826119364E-4 3.8311556087064545E-5 16-17 1.053567792394275E-4 1.9155778043532273E-5 0.0 1.1493466826119364E-4 3.8311556087064545E-5 18-19 1.2451255728295976E-4 5.746733413059682E-5 0.0 1.5324622434825818E-4 3.8311556087064545E-5 20-21 2.2029144750062115E-4 7.662311217412909E-5 0.0 3.7353767184887933E-4 3.8311556087064545E-5 22-23 2.490251145659195E-4 9.577889021766136E-5 0.0 0.0014462612422866865 3.8311556087064545E-5 24-25 2.681808926094518E-4 1.1493466826119364E-4 0.0 0.003735376718488793 3.8311556087064545E-5 26-27 3.256482267400486E-4 1.1493466826119364E-4 0.0 0.006081959528821497 4.788944510883068E-5 28-29 3.6395978282711315E-4 1.1493466826119364E-4 0.0 0.016646371119829542 6.704522315236295E-5 30-31 3.6395978282711315E-4 1.1493466826119364E-4 0.0 0.0499199575814451 8.620100119589523E-5 32-33 3.8311556087064545E-4 1.340904463047259E-4 0.0 0.10907300017987276 9.577889021766136E-5 34-35 3.8311556087064545E-4 1.9155778043532273E-4 0.0 0.18867483583977113 9.577889021766136E-5 36-37 3.8311556087064545E-4 2.2029144750062115E-4 0.0 0.31108025753794233 1.1493466826119364E-4 38-39 3.8311556087064545E-4 2.394472255441534E-4 0.0 0.5016419375150014 1.2451255728295976E-4 40-41 4.022713389141777E-4 2.490251145659195E-4 0.0 0.7347869120828326 1.340904463047259E-4 42-43 4.118492279359438E-4 2.5860300358768564E-4 0.0 0.94556751578484 1.340904463047259E-4 44-45 4.2142711695771E-4 2.681808926094518E-4 0.0 1.1842676659852955 1.4366833532649206E-4 46-47 4.310050059794761E-4 2.681808926094518E-4 0.0 1.4449394936016828 1.819798914135566E-4 48-49 4.4058289500124224E-4 2.681808926094518E-4 0.0 1.7225450290085524 2.10713558478855E-4 50-51 4.4058289500124224E-4 2.8733667065298406E-4 0.0 2.0365369648091116 2.2986933652238727E-4 52-53 4.4058289500124224E-4 2.8733667065298406E-4 0.0 2.3412191924805144 2.2986933652238727E-4 54-55 4.4058289500124224E-4 2.8733667065298406E-4 0.0 2.6339961040978617 2.2986933652238727E-4 56-57 4.4058289500124224E-4 2.9691455967475024E-4 0.0 2.963178571886942 2.394472255441534E-4 58-59 4.4058289500124224E-4 3.256482267400486E-4 0.0 3.3287282842916683 2.5860300358768564E-4 60-61 4.4058289500124224E-4 3.256482267400486E-4 0.0 3.7029842978171796 2.681808926094518E-4 62-63 4.4058289500124224E-4 3.256482267400486E-4 0.0 4.0792133564811754 2.681808926094518E-4 64-65 4.5973867304477454E-4 4.2142711695771E-4 0.0 4.464665922273133 2.7775878163121794E-4 66-67 4.5973867304477454E-4 4.4058289500124224E-4 0.0 4.880422928929956 3.0649244869651636E-4 68-69 4.5973867304477454E-4 4.4058289500124224E-4 0.0 5.328409532145024 3.256482267400486E-4 70-71 4.5973867304477454E-4 4.501607840230084E-4 0.0 5.8368326150874354 3.448040047835809E-4 72-73 4.5973867304477454E-4 4.5973867304477454E-4 0.0 6.335639497451995 4.310050059794761E-4 74-75 4.5973867304477454E-4 4.788944510883068E-4 0.0 6.821056490964123 4.4058289500124224E-4 76-77 4.5973867304477454E-4 4.98050229131839E-4 0.0 7.335446598767096 4.5973867304477454E-4 78-79 4.5973867304477454E-4 4.98050229131839E-4 0.0 7.866205318908266 4.788944510883068E-4 80-81 4.5973867304477454E-4 4.98050229131839E-4 0.0 8.448713373434039 4.788944510883068E-4 82-83 4.5973867304477454E-4 5.076281181536052E-4 0.0 9.073048069317863 4.788944510883068E-4 84-85 4.5973867304477454E-4 5.459396742406698E-4 0.0 9.684653750691762 4.788944510883068E-4 86-87 4.5973867304477454E-4 5.65095452284202E-4 0.0 10.300952597686326 4.98050229131839E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCGC 270 5.4569682E-11 22.388542 9 CTAACGC 1035 0.0 20.666346 3 TCTAACG 1040 0.0 19.225664 2 GTACTAG 1895 0.0 19.13991 1 GTACATA 1325 0.0 18.951012 1 CATGGGG 12585 0.0 18.732723 4 TAGTACT 2110 0.0 18.731936 4 ACGTTAA 435 0.0 18.172132 8 TACATGA 2030 0.0 18.095778 2 GTATAAG 1995 0.0 17.947433 1 CGGGCGT 1270 0.0 17.940666 6 TACGGGC 1300 0.0 17.884336 4 GTATAAT 1510 0.0 17.86099 1 CGTTAAC 445 0.0 17.76377 9 GTACATG 38515 0.0 17.43379 1 GGCGTCG 1380 0.0 17.184515 8 TACATGG 37595 0.0 16.957176 2 GTACAAG 2470 0.0 16.755121 1 GTATCAA 26595 0.0 16.225664 1 ATACGGG 1580 0.0 16.186457 3 >>END_MODULE