FastQCFastQC Report
Fri 27 May 2016
ERR765258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5220357
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143150.2742149626931645No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT141620.27128412865250406No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA133480.2556913253250688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93930.1799302231628986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73620.1410248379564846No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52710.1009701060674586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4850.038.3516042
GTATCAA357800.027.542851
CTAACGC8650.026.8793773
TCTAACG9100.026.0611782
CAACGCA432550.022.5868175
ATCAACG435500.022.465853
ATCACGC8350.022.2760813
AACGCAG450800.022.1252356
TATCAAC447700.022.0821552
GTATAAG21500.022.07421
AGTACTT235400.021.96832312-13
TCAACGC451200.021.7047394
GTACTAG20150.020.7821621
TCACGCA8850.020.492114
ACGCAGA478950.020.3976617
CGCAGAG482150.020.2815728
ACGCCTA11550.020.1294786
TAGTACT22650.019.5038644
GAGTACT225300.019.41315812-13
GCAGAGT508550.019.2290769
Produced by FastQC (version 0.11.5)