##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765258_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5220357 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34584722845583 33.0 33.0 33.0 33.0 33.0 2 32.31039812028181 33.0 33.0 33.0 33.0 33.0 3 32.34069949622219 33.0 33.0 33.0 33.0 33.0 4 36.21094074600645 37.0 37.0 37.0 33.0 37.0 5 36.195317676549706 37.0 37.0 37.0 33.0 37.0 6 36.220811335316725 37.0 37.0 37.0 33.0 37.0 7 36.113178274972384 37.0 37.0 37.0 33.0 37.0 8 36.11998432291125 37.0 37.0 37.0 33.0 37.0 9 36.1351804100754 37.0 37.0 37.0 33.0 37.0 10-11 36.096367451498054 37.0 37.0 37.0 33.0 37.0 12-13 36.078799974790996 37.0 37.0 37.0 33.0 37.0 14-15 37.57272175063889 40.0 37.0 40.0 33.0 40.0 16-17 37.576581161020215 40.0 37.0 40.0 33.0 40.0 18-19 37.627086998073125 40.0 37.0 40.0 33.0 40.0 20-21 37.613350676974775 40.0 37.0 40.0 33.0 40.0 22-23 37.58908040197251 40.0 37.0 40.0 33.0 40.0 24-25 37.57309844901412 40.0 37.0 40.0 33.0 40.0 26-27 37.452888087921956 40.0 37.0 40.0 33.0 40.0 28-29 37.354519432291696 38.5 37.0 40.0 33.0 40.0 30-31 37.19193965087062 37.0 37.0 40.0 33.0 40.0 32-33 37.07669063629173 37.0 37.0 40.0 33.0 40.0 34-35 36.98337948918053 37.0 37.0 40.0 33.0 40.0 36-37 36.84371739327406 37.0 37.0 40.0 33.0 40.0 38-39 36.72542538757406 37.0 37.0 40.0 33.0 40.0 40-41 36.559489513839765 37.0 37.0 40.0 33.0 40.0 42-43 36.39937029210837 37.0 37.0 40.0 33.0 40.0 44-45 36.11100706331004 37.0 37.0 40.0 33.0 40.0 46-47 35.86283026237477 37.0 37.0 40.0 30.0 40.0 48-49 35.58428178379371 37.0 37.0 40.0 27.0 40.0 50-51 35.38702056200371 37.0 37.0 40.0 27.0 40.0 52-53 35.23639829613185 37.0 35.0 40.0 27.0 40.0 54-55 35.0566203614044 37.0 33.0 40.0 27.0 40.0 56-57 34.865612064462255 37.0 33.0 40.0 27.0 40.0 58-59 34.65428772400048 37.0 33.0 40.0 27.0 40.0 60-61 34.48673855063936 37.0 33.0 37.0 27.0 40.0 62-63 34.268553185155724 37.0 33.0 37.0 27.0 40.0 64-65 34.044932559210025 37.0 33.0 37.0 27.0 40.0 66-67 33.816775461908065 37.0 33.0 37.0 27.0 40.0 68-69 33.60243121303773 37.0 33.0 37.0 27.0 40.0 70-71 33.41414456904001 37.0 33.0 37.0 27.0 38.5 72-73 33.19911847025021 37.0 33.0 37.0 27.0 37.0 74-75 32.96219904117669 37.0 33.0 37.0 27.0 37.0 76-77 32.78064095999565 37.0 33.0 37.0 24.5 37.0 78-79 32.60807057831485 35.0 33.0 37.0 22.0 37.0 80-81 32.44272633078542 33.0 33.0 37.0 22.0 37.0 82-83 32.33765506841773 33.0 33.0 37.0 22.0 37.0 84-85 32.204193314748395 33.0 33.0 37.0 22.0 37.0 86-87 32.096396759838456 33.0 33.0 37.0 22.0 37.0 88-89 31.987195320166798 33.0 33.0 37.0 22.0 37.0 90-91 31.87693667310492 33.0 33.0 37.0 22.0 37.0 92-93 31.799209804999926 33.0 33.0 37.0 22.0 37.0 94-95 31.701177907947674 33.0 33.0 37.0 22.0 37.0 96-97 31.64400097541222 33.0 33.0 37.0 22.0 37.0 98-99 30.856437117231636 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07236700206625812 1101 2 0.13182665818126793 1101 3 0.15954079229018703 1101 4 0.17769037805754806 1101 5 0.14815064352060148 1101 6 0.12753623926170832 1101 7 0.169662526373763 1101 8 0.17435275889719293 1101 9 0.1409199933335401 1101 10-11 0.12357814295069147 1101 12-13 0.14145074386263445 1101 14-15 0.22437860834725853 1101 16-17 0.23198890777339187 1101 18-19 0.23240388921846034 1101 20-21 0.27934312797385275 1101 22-23 0.23199263990589003 1101 24-25 0.3511879156456956 1101 26-27 0.3329890261137791 1101 28-29 0.25423414378582265 1101 30-31 0.23831931634672543 1101 32-33 0.305827643353652 1101 34-35 0.34017398946314614 1101 36-37 0.30864205321211813 1101 38-39 0.2239511299207635 1101 40-41 0.2248534383447165 1101 42-43 0.25093481049161426 1101 44-45 0.28755349635210337 1101 46-47 0.3519982553419112 1101 48-49 0.3394073243071034 1101 50-51 0.3518465960861832 1101 52-53 0.29994392711405027 1101 54-55 0.2469650446372924 1101 56-57 0.39529645650935663 1101 58-59 0.36241221217313324 1101 60-61 0.2922538010369138 1101 62-63 0.3130340275647967 1101 64-65 0.22142909440565717 1101 66-67 0.412301506845246 1101 68-69 0.3796833550912453 1101 70-71 0.2508631258738063 1101 72-73 0.20379574420704216 1101 74-75 0.2791115468374201 1101 76-77 0.36101155232753257 1101 78-79 0.26473170587831163 1101 80-81 0.30835519469621886 1101 82-83 0.26550929304600857 1101 84-85 0.3169114943871634 1101 86-87 0.2555015624679058 1101 88-89 0.21755503331661785 1101 90-91 0.23029662412448815 1101 92-93 0.24826750364198347 1101 94-95 0.26013957676091337 1101 96-97 0.32507750345312303 1101 98-99 0.3953116125533853 1102 1 -0.015815469247733915 1102 2 0.1419435591278102 1102 3 0.11220131040526127 1102 4 0.0988609586905369 1102 5 0.07995188584814628 1102 6 0.08394366051430069 1102 7 0.058575314324066596 1102 8 0.19388598213827635 1102 9 0.10680231030287501 1102 10-11 0.08056100663293364 1102 12-13 0.13864964953428682 1102 14-15 0.15240059861504562 1102 16-17 0.16858672396096352 1102 18-19 0.1343529014228153 1102 20-21 0.1860163031055322 1102 22-23 0.18216146213895712 1102 24-25 0.21361955350869266 1102 26-27 0.20325697741716198 1102 28-29 0.10542067462476723 1102 30-31 0.17708635225619673 1102 32-33 0.1850832994878715 1102 34-35 0.27685669566945137 1102 36-37 0.26370472631338515 1102 38-39 0.24518309752635048 1102 40-41 0.2561215381998778 1102 42-43 0.2920695158244726 1102 44-45 0.3618844814990396 1102 46-47 0.3961277571261448 1102 48-49 0.3859619591731942 1102 50-51 0.3598291588889211 1102 52-53 0.32494994332286353 1102 54-55 0.28520913777014556 1102 56-57 0.2614956271428568 1102 58-59 0.3127269844546774 1102 60-61 0.20937501035336226 1102 62-63 0.31129010982987637 1102 64-65 0.2420915598107385 1102 66-67 0.36254501940127426 1102 68-69 0.39311729840783016 1102 70-71 0.2667960176777342 1102 72-73 0.14260180604049566 1102 74-75 0.3987876394965184 1102 76-77 0.4050720047040741 1102 78-79 0.31253321325981176 1102 80-81 0.3463960306863072 1102 82-83 0.3606160684345241 1102 84-85 0.3852673227696144 1102 86-87 0.3071547328418731 1102 88-89 0.35240795094074784 1102 90-91 0.31399493618765817 1102 92-93 0.35000847889130426 1102 94-95 0.41976486158108983 1102 96-97 0.44186662172695534 1102 98-99 0.29475902849384994 1103 1 0.011470968840484375 1103 2 0.10851683416434099 1103 3 0.10304601216310516 1103 4 0.07407010182841134 1103 5 0.040163008085997376 1103 6 0.05265673479529909 1103 7 0.05100837301547756 1103 8 0.15075695515623977 1103 9 0.08409075485189987 1103 10-11 0.10944455712004242 1103 12-13 0.06983534018545612 1103 14-15 0.18016206629855702 1103 16-17 0.14063287924765433 1103 18-19 0.22374062382836257 1103 20-21 0.12226681764111902 1103 22-23 0.1215087817201308 1103 24-25 0.17161688065969827 1103 26-27 0.16271256075915375 1103 28-29 0.11875312025120621 1103 30-31 0.23316896858380431 1103 32-33 0.15600544151246254 1103 34-35 0.2331937677080873 1103 36-37 0.23929611543484697 1103 38-39 0.07587077548573973 1103 40-41 0.13507628764460833 1103 42-43 0.12077864102580804 1103 44-45 0.1364402328365273 1103 46-47 0.20865097582976233 1103 48-49 0.21177839111434338 1103 50-51 0.23922339925197633 1103 52-53 0.19848312927253176 1103 54-55 0.15622567465527482 1103 56-57 0.17843740694654997 1103 58-59 0.25624382078888885 1103 60-61 0.17782894718146736 1103 62-63 0.23035616920773805 1103 64-65 0.23024815130808207 1103 66-67 0.29161048900127184 1103 68-69 0.2778597752438472 1103 70-71 0.06988618012201187 1103 72-73 0.14828513666815013 1103 74-75 0.23341453202986884 1103 76-77 0.2335640825279981 1103 78-79 0.17685122635288053 1103 80-81 0.2581079552044656 1103 82-83 0.14893086632616104 1103 84-85 0.20214720629761018 1103 86-87 0.19917719669061995 1103 88-89 0.2205041449278049 1103 90-91 0.19605672058797197 1103 92-93 0.20124734683395928 1103 94-95 0.10050890284851377 1103 96-97 0.1764100333304981 1103 98-99 0.2344474920130466 1104 1 0.0217994718602057 1104 2 0.07917959334804436 1104 3 -0.03125975940944414 1104 4 0.018127559193665377 1104 5 -0.04027695413451937 1104 6 0.046213243574840135 1104 7 0.060698170762854886 1104 8 0.09868925385463712 1104 9 0.05717536007539081 1104 10-11 0.014643532422809358 1104 12-13 -0.04711568461803495 1104 14-15 0.13807097747881159 1104 16-17 0.10363385439801931 1104 18-19 0.10657299086621208 1104 20-21 0.1211696520437755 1104 22-23 0.15436212724183207 1104 24-25 0.10134837124624596 1104 26-27 0.11772468031161765 1104 28-29 0.06353003942575697 1104 30-31 0.028194669503484704 1104 32-33 0.10924161552276246 1104 34-35 0.03988289845504056 1104 36-37 0.14926345305880062 1104 38-39 0.11227082060010929 1104 40-41 0.09680145371920901 1104 42-43 0.18721967767257297 1104 44-45 0.1582340768810866 1104 46-47 0.09114053744016815 1104 48-49 0.1087973667753559 1104 50-51 0.010119661711932793 1104 52-53 0.09206384224346209 1104 54-55 0.014199752717694025 1104 56-57 0.13850719304191728 1104 58-59 0.06486058123130789 1104 60-61 0.07702109339572871 1104 62-63 0.07085813787602291 1104 64-65 0.07477272148444314 1104 66-67 0.12612108161763302 1104 68-69 0.15101331193994838 1104 70-71 0.08900442499708561 1104 72-73 0.14562247827831953 1104 74-75 0.1776929769261315 1104 76-77 0.1683852993954318 1104 78-79 0.192733077110546 1104 80-81 0.2359220700505844 1104 82-83 0.21412976269396466 1104 84-85 0.1775844908013653 1104 86-87 0.1993139666902337 1104 88-89 0.23556119365284545 1104 90-91 0.22929277164189088 1104 92-93 0.25233132118495405 1104 94-95 0.16489224168199357 1104 96-97 0.1533063015587821 1104 98-99 0.246742234549977 1105 1 -0.0583847010518852 1105 2 0.0996888790432564 1105 3 0.04527290885537383 1105 4 0.039498267721469915 1105 5 0.024753538737208203 1105 6 0.058003225045140994 1105 7 0.019889297670808048 1105 8 0.08981659112070162 1105 9 0.009419883166103205 1105 10-11 -0.025076056872066488 1105 12-13 -0.007308645448866002 1105 14-15 0.019154325568528918 1105 16-17 0.05000620535086142 1105 18-19 0.08014333356481274 1105 20-21 0.03374899069657289 1105 22-23 0.006124787164374368 1105 24-25 0.022034456854122197 1105 26-27 -0.07082253193302535 1105 28-29 -0.01288041064829315 1105 30-31 -0.030558683484933624 1105 32-33 -0.01601865178028561 1105 34-35 0.056198482724056475 1105 36-37 0.04920602033495669 1105 38-39 0.013576971015524464 1105 40-41 -0.06370323681390033 1105 42-43 0.0019247542186491273 1105 44-45 0.014745763827406222 1105 46-47 -0.039166683567295024 1105 48-49 0.04963991524942202 1105 50-51 0.0214301956562295 1105 52-53 -0.05812437074700938 1105 54-55 -0.14831249064261698 1105 56-57 0.0026563302540481004 1105 58-59 0.06686518630993987 1105 60-61 -0.059758861950264475 1105 62-63 -0.06121102803599143 1105 64-65 -0.00772765620528304 1105 66-67 0.031040899681990197 1105 68-69 -0.043844417771822464 1105 70-71 -0.07662432931974195 1105 72-73 -0.05836734627501272 1105 74-75 -0.04193191012596742 1105 76-77 0.005417817839038719 1105 78-79 0.012314001893948046 1105 80-81 0.01184245465709921 1105 82-83 -0.046264482502657245 1105 84-85 -0.07527829458642543 1105 86-87 0.025886081877608547 1105 88-89 0.058983631933106295 1105 90-91 -0.035794916622119644 1105 92-93 0.012957365235926943 1105 94-95 0.00914539874338871 1105 96-97 -0.04643128230504345 1105 98-99 0.09829694214828422 1106 1 -0.009996400041714537 1106 2 0.11752000588797529 1106 3 0.0380731080503125 1106 4 0.04134180807088228 1106 5 0.027322216977509584 1106 6 0.051721957851825096 1106 7 0.009357610478026857 1106 8 0.04746594313123609 1106 9 0.0015983962542023278 1106 10-11 0.007583966928933705 1106 12-13 0.0034426994391054677 1106 14-15 0.08161004451024922 1106 16-17 0.04893704594153547 1106 18-19 0.03840008055715316 1106 20-21 -0.00405017782301087 1106 22-23 0.030542896003105113 1106 24-25 0.10810775995076938 1106 26-27 0.0747814500266415 1106 28-29 0.06338594411813858 1106 30-31 0.04614611472113239 1106 32-33 -0.021325831753813418 1106 34-35 0.0981412351078319 1106 36-37 0.11965970015334904 1106 38-39 0.0587944748445679 1106 40-41 0.0420384376968741 1106 42-43 -0.004140032727129039 1106 44-45 0.14998136462482137 1106 46-47 0.18657156757615923 1106 48-49 0.08628222680445674 1106 50-51 -0.022460110456414384 1106 52-53 0.050691181402058305 1106 54-55 0.05848359105308276 1106 56-57 0.14498529474957422 1106 58-59 0.20040704494124384 1106 60-61 0.14932000159110004 1106 62-63 0.09674085541605848 1106 64-65 0.1611011330429193 1106 66-67 0.20477996874480198 1106 68-69 0.17944444981716146 1106 70-71 0.059164590734084754 1106 72-73 0.13259691775451188 1106 74-75 0.15029168724962716 1106 76-77 0.24177960658068542 1106 78-79 0.1281361216943111 1106 80-81 0.09082387193825525 1106 82-83 0.11032856176588979 1106 84-85 0.1271919950957141 1106 86-87 0.13805325014749315 1106 88-89 0.22449389485256077 1106 90-91 0.05281585183369586 1106 92-93 0.23745118009021837 1106 94-95 0.113821549436107 1106 96-97 0.19155407873547503 1106 98-99 0.2021632870542831 1107 1 -0.02082066723600917 1107 2 0.050862113163006484 1107 3 0.07237035280218151 1107 4 -0.04295695782813169 1107 5 -0.005166729474815668 1107 6 -0.012066844270904653 1107 7 0.040797782127590665 1107 8 0.022254584621997253 1107 9 0.05041285384331928 1107 10-11 -0.03515786855808045 1107 12-13 -0.037836348163523326 1107 14-15 0.1051198110770244 1107 16-17 0.07113764152533264 1107 18-19 0.015847462648345356 1107 20-21 0.06391415132033273 1107 22-23 0.08830017470674534 1107 24-25 0.08270797900178906 1107 26-27 0.04452234047730741 1107 28-29 0.044120936455669835 1107 30-31 0.058019280685108754 1107 32-33 0.06786472020441181 1107 34-35 -0.0013869919722822033 1107 36-37 0.08841340548838872 1107 38-39 0.012988235212766597 1107 40-41 -0.04095863898793084 1107 42-43 0.040535730603608044 1107 44-45 0.00145087005832778 1107 46-47 0.07469091109280157 1107 48-49 0.0018542873655391645 1107 50-51 -0.0038455208089729354 1107 52-53 -0.04401705834717973 1107 54-55 -0.12449166891067165 1107 56-57 0.004453000591624345 1107 58-59 0.05297214092358615 1107 60-61 0.025370639543872642 1107 62-63 -0.0775677452898833 1107 64-65 -0.047097502930007806 1107 66-67 -0.06831736714172365 1107 68-69 -0.07920125173750137 1107 70-71 -0.057255865944334516 1107 72-73 -0.10645478978492662 1107 74-75 0.07833786898508066 1107 76-77 0.03489862967928303 1107 78-79 -0.015488505628290739 1107 80-81 0.041974944250739554 1107 82-83 -0.008609488226149153 1107 84-85 -0.021916163482899265 1107 86-87 -0.028865587129054404 1107 88-89 -0.0356190282272415 1107 90-91 -0.07257359027341437 1107 92-93 0.15407322595466155 1107 94-95 -0.056890573774072806 1107 96-97 -0.020883511572609592 1107 98-99 0.029005704698754187 1108 1 -0.019867571888916302 1108 2 0.0024247509148906943 1108 3 0.004296547423791708 1108 4 -0.02851589771189822 1108 5 0.06622537805699835 1108 6 0.039525936385317095 1108 7 -0.015362615944347624 1108 8 -0.07138098144869787 1108 9 -0.002083513555874106 1108 10-11 -0.05442660852777692 1108 12-13 0.026023250688083976 1108 14-15 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-0.42508417613884575 2215 84-85 -0.42577041810318406 2215 86-87 -0.4762772490050473 2215 88-89 -0.3463795902088549 2215 90-91 -0.3684086979973422 2215 92-93 -0.2810341562858518 2215 94-95 -0.27302625540720626 2215 96-97 -0.3030261412334525 2215 98-99 -0.37164536941776305 2216 1 -0.16084916185660347 2216 2 -0.25454194093632054 2216 3 -0.2228599270936229 2216 4 -0.290585403272118 2216 5 -0.22737432934663104 2216 6 -0.28625266484708334 2216 7 -0.1986079615485039 2216 8 -0.30615223866394814 2216 9 -0.2567167113482398 2216 10-11 -0.29297787364008343 2216 12-13 -0.23361604075283537 2216 14-15 -0.5688384096652683 2216 16-17 -0.4599437444965062 2216 18-19 -0.4339814068648735 2216 20-21 -0.48672471792109206 2216 22-23 -0.547715172124235 2216 24-25 -0.5622976323778559 2216 26-27 -0.598532727842155 2216 28-29 -0.4795597809412797 2216 30-31 -0.541049871865404 2216 32-33 -0.43553494466142695 2216 34-35 -0.5065632013028107 2216 36-37 -0.5250855496667199 2216 38-39 -0.49155770130137455 2216 40-41 -0.47474078787927 2216 42-43 -0.4822940592327569 2216 44-45 -0.5690521646061271 2216 46-47 -0.5759972611744928 2216 48-49 -0.4605501011094404 2216 50-51 -0.44646021987404794 2216 52-53 -0.5321803578700184 2216 54-55 -0.481826829300374 2216 56-57 -0.6391509036391412 2216 58-59 -0.6445743224376272 2216 60-61 -0.5885963072019038 2216 62-63 -0.5200205818889074 2216 64-65 -0.618900580955831 2216 66-67 -0.5994315823418503 2216 68-69 -0.44073416972444335 2216 70-71 -0.5201105395867458 2216 72-73 -0.5652131009792996 2216 74-75 -0.6167282571181545 2216 76-77 -0.5876524874451192 2216 78-79 -0.42044767893031576 2216 80-81 -0.5857783216006567 2216 82-83 -0.6433874634267269 2216 84-85 -0.658420155447768 2216 86-87 -0.7201376083571631 2216 88-89 -0.7006948979989858 2216 90-91 -0.6985512202896054 2216 92-93 -0.5292686880014479 2216 94-95 -0.6412849019034077 2216 96-97 -0.716000434014525 2216 98-99 -0.6986588473105684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 17.0 10 54.0 11 106.0 12 171.0 13 379.0 14 870.0 15 2361.0 16 5431.0 17 10501.0 18 17516.0 19 25039.0 20 32904.0 21 42201.0 22 50884.0 23 48408.0 24 38984.0 25 35725.0 26 40812.0 27 52514.0 28 69253.0 29 92271.0 30 122614.0 31 163552.0 32 217780.0 33 290721.0 34 392027.0 35 556803.0 36 924803.0 37 1432534.0 38 552478.0 39 642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6706001568975 19.269431878099113 13.119053693023314 24.940914271980073 2 15.984998727098548 21.338540640036687 37.4818044053309 25.194656227533862 3 17.91249142539485 26.656778454040598 29.85094697546547 25.579783145099082 4 13.146150732603154 17.56094458673995 36.845200433610195 32.4477042470467 5 13.810539390773306 37.411287500050285 33.62033971425413 15.157833394922275 6 31.023452993732036 37.49835499756051 17.393523086639476 14.084668922067973 7 26.639863902028154 32.22658910109021 22.767408435859846 18.366138561021785 8 27.101939579994244 32.7321867067712 20.237926256767498 19.927947456467056 9 27.404945677086832 13.987242634938568 20.04895450636805 38.55885718160654 10-11 25.294975037147843 25.200853121730947 27.52284949094478 21.981322350176434 12-13 26.75962582635632 23.169239191879022 27.44934877059174 22.621786211172914 14-15 24.15033788411007 23.544814924713823 25.909802556605673 26.395044634570436 16-17 23.029560389574126 26.155394603236847 26.59548107267078 24.219563934518245 18-19 23.419708036887414 24.913017734363642 29.20339895067675 22.463875278072194 20-21 24.542841692324284 24.2265600351854 28.533687511577295 22.69691076091302 22-23 23.973992583265858 23.986721597775784 28.305631204915677 23.733654614042678 24-25 23.505547609100297 24.596076475229566 28.401869833806387 23.49650608186375 26-27 23.70632889666358 24.448414160181 28.24488823273964 23.600368710415783 28-29 23.40523837737534 24.33837379320993 28.76038937566914 23.495998453745596 30-31 23.906609620628522 24.240926605425503 28.488222521691576 23.3642412522544 32-33 23.56874252086591 24.483986440007836 28.087753385448543 23.85951765367771 34-35 23.75279123630817 24.45194840123003 28.492026503168272 23.30323385929353 36-37 24.064446167187416 24.32303001499706 28.00047966068221 23.612044157133315 38-39 24.001337456423 24.069225533809277 28.123718358725274 23.805718651042447 40-41 23.786380893107502 24.219272743224266 28.247790333113233 23.746556030555 42-43 24.325481954586632 24.224416069628955 27.63594520451379 23.81415677127062 44-45 24.083190096003012 24.56462268768209 27.250751241725425 24.101435974589478 46-47 23.94776832312426 24.596766083239135 27.196798992865812 24.25866660077079 48-49 24.72548333380265 24.75623793545154 26.458065990506014 24.060212740239795 50-51 23.950210684824814 25.252113983775438 26.087957202926926 24.709718128472822 52-53 23.81505709283867 25.35058426080822 25.8227358780252 25.01162276832791 54-55 24.008310159630845 25.244020667552046 25.84980299240071 24.897866180416397 56-57 24.174065107041528 24.804204003672545 25.86155506223042 25.160175827055507 58-59 23.756155350693003 24.797319876525677 26.573520409956675 24.873004362824645 60-61 23.96543952836942 25.039935008276252 26.18732780152775 24.807297661826578 62-63 23.706348052441626 24.95910720282157 26.035336280641342 25.299208464095464 64-65 23.415362206071347 24.802614074094933 26.208130976483023 25.573892743350697 66-67 24.04678453983128 24.772855572904305 26.14747420530818 25.032885681956234 68-69 23.879334306063743 24.392709157630406 26.2584148938473 25.469541642458555 70-71 23.42795713013497 24.583108013494094 26.238435417347894 25.750499439023038 72-73 24.056649765523698 24.644435236900463 26.19572761019984 25.103187387375996 74-75 24.179717748945116 24.646793487604523 26.004891432139882 25.168597331310483 76-77 23.74712650944156 24.73452208515924 26.179808459184585 25.338542946214616 78-79 23.77128519852968 24.906419163608284 26.07189590435743 25.250399733504615 80-81 23.491264148581454 24.881428094312735 26.20313884753634 25.424168909569474 82-83 23.590122628598255 24.76930454845373 26.024506180756045 25.616066642191964 84-85 23.751559048650364 24.886352991793046 25.552996543705753 25.809091415850833 86-87 23.392690229678692 24.655947452974335 25.93776677631204 26.013595541034935 88-89 23.49501689640581 24.339367868880597 26.042407957641085 26.123207277072513 90-91 23.607272867981983 24.51982465014267 25.92819023174214 25.944712250133207 92-93 23.418024863809936 24.655573898554827 25.761013110796014 26.165388126839222 94-95 22.839379234850416 24.66092223252708 26.292236681056323 26.207461851566176 96-97 23.41600128652559 24.41315439849134 25.931335049020056 26.23950926596302 98-99 23.16825253612205 24.5650532127086 25.864254555154396 26.40243969601495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 52.5 2 223.0 3 371.5 4 281.5 5 270.0 6 529.0 7 1448.0 8 2619.0 9 2575.5 10 1650.0 11 1139.5 12 1129.0 13 1289.0 14 1549.5 15 2001.5 16 2478.5 17 2922.0 18 3591.5 19 4298.0 20 5297.5 21 6655.5 22 8275.0 23 10074.0 24 12226.5 25 15026.0 26 18877.5 27 22710.0 28 27021.0 29 33065.5 30 38109.5 31 44707.0 32 53233.0 33 60539.0 34 66439.5 35 73817.0 36 81351.0 37 90859.0 38 101628.5 39 101719.0 40 102260.0 41 110772.0 42 121747.5 43 137077.5 44 155578.0 45 175934.0 46 197775.0 47 219933.0 48 233452.5 49 245852.0 50 264270.0 51 256613.0 52 237150.0 53 232649.0 54 223596.0 55 211088.5 56 190934.0 57 172411.0 58 161426.0 59 142078.0 60 117528.0 61 91378.5 62 74374.5 63 59613.5 64 42850.5 65 31140.5 66 22239.5 67 16664.0 68 12531.0 69 10547.0 70 9159.5 71 7871.5 72 8180.0 73 6955.0 74 4548.0 75 3511.5 76 2606.5 77 1641.0 78 873.0 79 533.5 80 346.5 81 215.5 82 134.5 83 103.0 84 64.5 85 42.0 86 22.0 87 9.0 88 5.0 89 4.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05627967589189782 2 0.0 3 0.0 4 0.0 5 0.00505712540349252 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014558391313084526 16-17 0.0047218992877307055 18-19 7.662311217412908E-4 20-21 1.915577804353227E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.2986933652238727E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.577889021766135E-6 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 8.620100119589523E-5 76-77 1.4366833532649203E-4 78-79 7.662311217412908E-5 80-81 2.873366706529841E-5 82-83 9.577889021766135E-6 84-85 4.98050229131839E-4 86-87 8.141205668501215E-4 88-89 3.064924486965163E-4 90-91 9.673667911983798E-4 92-93 0.0015133064654390496 94-95 0.0011014572375031056 96-97 0.0018293768031573318 98-99 6.608743425018634E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5220357.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.95219157728652 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45641249117426 24.600539979032447 2 13.79442357173512 9.367018236115756 3 4.921215716738044 5.012581764235304 4 2.342769228248755 3.1816859863549367 5 1.2948497741473117 2.1981493797827856 6 0.8644635192973512 1.7610258611255394 7 0.6044230866133632 1.4365041901302227 8 0.4611231166976577 1.2524912319067458 9 0.3417508731942495 1.0442872006556503 >10 2.385068443179541 15.922978687019029 >50 0.2764479208764827 6.523381616705248 >100 0.22321660789185882 15.285797508737586 >500 0.022425297819928795 5.178128814263581 >1k 0.011071431028335375 6.010860705924538 >5k 1.6946067900513152E-4 0.4224264808072752 >10k+ 1.6946067900513152E-4 0.8021423572034997 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14315 0.2742149626931645 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 14162 0.27128412865250406 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13348 0.2556913253250688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9393 0.1799302231628986 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7362 0.1410248379564846 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5271 0.1009701060674586 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.9155778043532273E-5 0.0 2 0.0 1.9155778043532273E-5 0.0 1.9155778043532273E-5 1.9155778043532273E-5 3 0.0 1.9155778043532273E-5 0.0 1.9155778043532273E-5 1.9155778043532273E-5 4 0.0 1.9155778043532273E-5 0.0 3.8311556087064545E-5 1.9155778043532273E-5 5 0.0 5.746733413059682E-5 0.0 3.8311556087064545E-5 1.9155778043532273E-5 6 0.0 5.746733413059682E-5 0.0 3.8311556087064545E-5 1.9155778043532273E-5 7 0.0 5.746733413059682E-5 0.0 5.746733413059682E-5 1.9155778043532273E-5 8 1.9155778043532273E-5 5.746733413059682E-5 0.0 5.746733413059682E-5 1.9155778043532273E-5 9 1.9155778043532273E-5 5.746733413059682E-5 0.0 5.746733413059682E-5 1.9155778043532273E-5 10-11 1.9155778043532273E-5 5.746733413059682E-5 0.0 9.577889021766136E-5 1.9155778043532273E-5 12-13 1.9155778043532273E-5 6.704522315236295E-5 0.0 9.577889021766136E-5 3.8311556087064545E-5 14-15 1.9155778043532273E-5 9.577889021766136E-5 0.0 9.577889021766136E-5 5.746733413059682E-5 16-17 1.9155778043532273E-5 9.577889021766136E-5 0.0 9.577889021766136E-5 7.662311217412909E-5 18-19 1.9155778043532273E-5 9.577889021766136E-5 0.0 1.340904463047259E-4 7.662311217412909E-5 20-21 1.9155778043532273E-5 9.577889021766136E-5 0.0 3.639597828271132E-4 9.577889021766136E-5 22-23 1.9155778043532273E-5 1.1493466826119364E-4 0.0 0.0014462612422866865 1.340904463047259E-4 24-25 3.8311556087064545E-5 1.1493466826119364E-4 0.0 0.003811999830662922 1.340904463047259E-4 26-27 3.8311556087064545E-5 1.1493466826119364E-4 0.0 0.0062256278641479885 1.340904463047259E-4 28-29 4.788944510883068E-5 1.1493466826119364E-4 0.0 0.016962441457547828 1.340904463047259E-4 30-31 5.746733413059682E-5 1.2451255728295976E-4 0.0 0.05082985703851288 1.340904463047259E-4 32-33 5.746733413059682E-5 1.5324622434825818E-4 0.0 0.11060546242335534 1.340904463047259E-4 34-35 5.746733413059682E-5 1.5324622434825818E-4 0.0 0.19109804176227796 1.9155778043532273E-4 36-37 5.746733413059682E-5 1.5324622434825818E-4 0.0 0.3154477749318677 1.9155778043532273E-4 38-39 5.746733413059682E-5 1.628241133700243E-4 0.0 0.5081932136058894 1.9155778043532273E-4 40-41 5.746733413059682E-5 1.9155778043532273E-4 0.0 0.7439433739876411 2.10713558478855E-4 42-43 7.662311217412909E-5 1.9155778043532273E-4 0.0 0.9569747816097636 2.10713558478855E-4 44-45 7.662311217412909E-5 2.10713558478855E-4 0.0 1.1985004090716402 2.10713558478855E-4 46-47 7.662311217412909E-5 2.2986933652238727E-4 0.0 1.4628405681833638 2.2986933652238727E-4 48-49 7.662311217412909E-5 2.2986933652238727E-4 0.0 1.7440952793075262 3.0649244869651636E-4 50-51 7.662311217412909E-5 2.490251145659195E-4 0.0 2.062119506386249 3.0649244869651636E-4 52-53 7.662311217412909E-5 2.490251145659195E-4 0.0 2.3709106484479894 3.160703377182825E-4 54-55 7.662311217412909E-5 2.5860300358768564E-4 0.0 2.668897931693177 3.256482267400486E-4 56-57 7.662311217412909E-5 2.681808926094518E-4 0.0 3.003750509783143 3.256482267400486E-4 58-59 9.577889021766136E-5 2.681808926094518E-4 0.0 3.374692573707124 3.4480400478358085E-4 60-61 1.053567792394275E-4 2.681808926094518E-4 0.0 3.754791099535913 3.6395978282711315E-4 62-63 1.340904463047259E-4 2.681808926094518E-4 0.0 4.137245786064057 3.8311556087064545E-4 64-65 1.340904463047259E-4 2.681808926094518E-4 0.0 4.529479497283424 3.8311556087064545E-4 66-67 1.340904463047259E-4 2.681808926094518E-4 0.0 4.952860503601574 3.8311556087064545E-4 68-69 1.340904463047259E-4 2.7775878163121794E-4 0.0 5.4094767848252525 3.8311556087064545E-4 70-71 1.628241133700243E-4 2.8733667065298406E-4 0.0 5.927918339684432 4.022713389141777E-4 72-73 1.819798914135566E-4 2.8733667065298406E-4 0.0 6.436561714074344 4.4058289500124224E-4 74-75 1.9155778043532273E-4 3.0649244869651636E-4 0.0 6.931163903158348 4.4058289500124224E-4 76-77 2.2986933652238727E-4 3.0649244869651636E-4 0.0 7.457171990344722 4.4058289500124224E-4 78-79 2.2986933652238727E-4 3.6395978282711315E-4 0.0 8.000678880773863 4.4058289500124224E-4 80-81 2.2986933652238727E-4 3.6395978282711315E-4 0.0 8.59699825126902 4.4058289500124224E-4 82-83 2.2986933652238727E-4 3.6395978282711315E-4 0.0 9.23414816266397 4.788944510883068E-4 84-85 2.2986933652238727E-4 3.6395978282711315E-4 0.0 9.860858175025195 4.788944510883068E-4 86-87 2.2986933652238727E-4 3.6395978282711315E-4 0.0 10.492213463561974 4.98050229131839E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 485 0.0 38.351604 2 GTATCAA 35780 0.0 27.54285 1 CTAACGC 865 0.0 26.879377 3 TCTAACG 910 0.0 26.061178 2 CAACGCA 43255 0.0 22.586817 5 ATCAACG 43550 0.0 22.46585 3 ATCACGC 835 0.0 22.276081 3 AACGCAG 45080 0.0 22.125235 6 TATCAAC 44770 0.0 22.082155 2 GTATAAG 2150 0.0 22.0742 1 AGTACTT 23540 0.0 21.968323 12-13 TCAACGC 45120 0.0 21.704739 4 GTACTAG 2015 0.0 20.782162 1 TCACGCA 885 0.0 20.49211 4 ACGCAGA 47895 0.0 20.397661 7 CGCAGAG 48215 0.0 20.281572 8 ACGCCTA 1155 0.0 20.129478 6 TAGTACT 2265 0.0 19.503864 4 GAGTACT 22530 0.0 19.413158 12-13 GCAGAGT 50855 0.0 19.229076 9 >>END_MODULE