FastQCFastQC Report
Fri 27 May 2016
ERR765255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12516784
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA310660.2481947439533989No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT309310.24711619214648106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172370.13771109256179542No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG128240.10245443238454863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG42950.018.8270661
GTATAAG48950.017.9599971
CATGGGG284400.017.6014674
GTACATG859850.017.5017051
TACATGG862500.016.8899272
TAGTACT50300.016.8202864
GTACAAG70400.015.7600181
TAGGCAT61300.015.71651655
GTATCAA616950.015.6976511
GTATAAA152800.015.691491
ACATGGG850450.015.602823
GTACATA35550.015.3403671
GTATTAG41500.014.9535131
CTAGGCA66250.014.8282284
GTATATA38150.014.5413531
GTCCTAC43200.014.3650641
GTATTAC22000.014.3175761
GTCCTAT48500.014.1523481
TAGGACT56200.014.13445954
TACACTA46900.014.0287225
Produced by FastQC (version 0.11.5)