##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765255_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12516784
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643716069559083	33.0	33.0	33.0	27.0	33.0
2	31.49587330100128	33.0	33.0	33.0	27.0	33.0
3	31.473994038724324	33.0	33.0	33.0	27.0	33.0
4	35.07663342277058	37.0	37.0	37.0	33.0	37.0
5	35.28331854252658	37.0	37.0	37.0	33.0	37.0
6	35.310370059913154	37.0	37.0	37.0	33.0	37.0
7	35.15817050130449	37.0	37.0	37.0	33.0	37.0
8	35.13625408890974	37.0	37.0	37.0	33.0	37.0
9	34.83806782956389	37.0	37.0	37.0	33.0	37.0
10-11	34.84124184774619	37.0	37.0	37.0	30.0	37.0
12-13	34.82688664276702	37.0	37.0	37.0	33.0	37.0
14-15	36.08910895162847	37.0	37.0	40.0	30.0	40.0
16-17	36.331405734891646	37.0	37.0	40.0	33.0	40.0
18-19	36.14988498643102	37.0	37.0	40.0	27.0	40.0
20-21	36.14181434304531	37.0	37.0	40.0	27.0	40.0
22-23	36.31897894059688	37.0	37.0	40.0	33.0	40.0
24-25	36.33729027360383	37.0	37.0	40.0	33.0	40.0
26-27	36.015256634611575	37.0	37.0	40.0	27.0	40.0
28-29	35.89888041528879	37.0	37.0	40.0	27.0	40.0
30-31	35.70488957067566	37.0	37.0	40.0	27.0	40.0
32-33	35.66880314464163	37.0	37.0	40.0	27.0	40.0
34-35	35.62147740985225	37.0	35.0	40.0	27.0	40.0
36-37	35.396665429394645	37.0	33.0	40.0	27.0	40.0
38-39	35.268198444584485	37.0	33.0	40.0	27.0	40.0
40-41	35.41331467412077	37.0	33.0	40.0	27.0	40.0
42-43	35.340251896972894	37.0	33.0	40.0	27.0	40.0
44-45	35.29182947472769	37.0	33.0	40.0	27.0	40.0
46-47	35.25609569518816	37.0	33.0	40.0	27.0	40.0
48-49	35.090724382557056	37.0	33.0	40.0	27.0	40.0
50-51	34.940479319607974	37.0	33.0	40.0	27.0	40.0
52-53	34.82454263011968	37.0	33.0	40.0	27.0	40.0
54-55	34.701578376682065	37.0	33.0	40.0	27.0	40.0
56-57	34.500557411552364	37.0	33.0	40.0	27.0	40.0
58-59	34.304019866444925	37.0	33.0	38.5	27.0	40.0
60-61	34.027948273294484	37.0	33.0	37.0	27.0	40.0
62-63	33.79620767602924	37.0	33.0	37.0	27.0	40.0
64-65	33.58950306244799	37.0	33.0	37.0	27.0	40.0
66-67	33.36060704570759	37.0	33.0	37.0	24.5	40.0
68-69	33.15088500368785	37.0	33.0	37.0	22.0	40.0
70-71	32.906225592772074	37.0	33.0	37.0	22.0	37.0
72-73	32.66241280507836	37.0	33.0	37.0	22.0	37.0
74-75	32.436650860157044	33.0	33.0	37.0	22.0	37.0
76-77	32.229239994874085	33.0	33.0	37.0	22.0	37.0
78-79	32.028362517081064	33.0	33.0	37.0	22.0	37.0
80-81	31.874178862557667	33.0	33.0	37.0	22.0	37.0
82-83	31.72594781534937	33.0	33.0	37.0	22.0	37.0
84-85	31.55643294635427	33.0	33.0	37.0	22.0	37.0
86-87	31.34747192250022	33.0	33.0	37.0	22.0	37.0
88-89	31.198158928044137	33.0	27.0	37.0	22.0	37.0
90-91	31.094883637841797	33.0	27.0	37.0	22.0	37.0
92-93	30.99207348309278	33.0	27.0	37.0	18.5	37.0
94-95	30.851658820668312	33.0	27.0	37.0	15.0	37.0
96-97	30.7417264690355	33.0	27.0	37.0	15.0	37.0
98-99	30.6007076578137	33.0	27.0	37.0	15.0	37.0
100	30.505765937959783	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	225.0
8	1251.0
9	2587.0
10	4199.0
11	7701.0
12	14034.0
13	23671.0
14	33047.0
15	38489.0
16	44621.0
17	52829.0
18	61801.0
19	73588.0
20	86411.0
21	101789.0
22	116648.0
23	125447.0
24	130687.0
25	143714.0
26	166776.0
27	200191.0
28	247088.0
29	305805.0
30	382425.0
31	474835.0
32	605050.0
33	792611.0
34	1039173.0
35	1394940.0
36	2036834.0
37	2797017.0
38	1010478.0
39	816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39242935043299	18.347173933391346	12.796394306070416	25.46400241010524
2	15.8322304788361	20.627799572933398	37.549696377717325	25.990273570513178
3	18.161258100862227	25.472475048145693	29.31929583429891	27.04697101669317
4	12.899483929544767	17.131979259371253	36.32413293634828	33.64440387473571
5	14.430981684801386	36.98313811461774	33.28171999157481	15.304160209006067
6	32.185955173937906	36.08111143688617	17.17762073981518	14.555312649360738
7	27.863957345090796	31.562834340543066	21.893461043344324	18.679747271021814
8	27.164534685695035	32.91041269557696	20.016025911193058	19.909026707534952
9	27.03865685331424	14.487667262157766	19.59608605751869	38.8775898270093
10-11	25.31559728534443	25.08920421475484	27.02977788128555	22.565420618615182
12-13	26.48695272087774	22.797216675261247	26.56716611086537	24.14866449299565
14-15	24.271334072197796	23.583865750372468	24.45730053695505	27.687499640474687
16-17	23.969466734494933	26.20863439316461	25.235649819833505	24.586249052506954
18-19	24.307888484922085	25.435690913063958	27.052233693174944	23.204186908839016
20-21	25.229002923482373	24.942655137543177	26.309200078669036	23.51914186030541
22-23	24.835015276688818	24.743485392775032	25.96712275656095	24.454376573975196
24-25	24.400742232154524	25.170852044634618	26.00367360165196	24.424732121558897
26-27	24.49560177437828	25.056132598239238	26.10921018940916	24.339055437973318
28-29	24.212799702728606	24.960138481534816	26.557452680220283	24.269609135516294
30-31	24.616198209342592	24.937112547050443	26.353792171859375	24.092897071747586
32-33	24.190000423203223	25.165361963237746	26.030147175214406	24.614490438344625
34-35	24.436745632729913	25.168593239101178	26.35618152829255	24.03847959987636
36-37	24.708338388854546	24.918943809300465	25.975176072014282	24.397541729830706
38-39	24.695836605411657	24.762032242015273	26.115517580972746	24.426613571600324
40-41	24.486938411986255	24.95279449703178	26.238250182211402	24.32201690877056
42-43	24.81063886883978	24.940239719005373	25.89197560298469	24.357145809170152
44-45	24.619778318282055	25.14414331846611	25.571701980528978	24.664376382722857
46-47	24.350487414738094	25.194070936529723	25.870302376129363	24.585139272602824
48-49	24.921582139690756	25.276812888381862	25.406551316970766	24.395053654956616
50-51	24.20404843493395	25.584843331956847	25.296640434047795	24.914467799061406
52-53	24.248951255108405	25.520028266129042	24.971199586793613	25.259820891968943
54-55	24.37265035491545	25.359597161699043	25.053699896075543	25.214052587309965
56-57	24.560458181590413	25.052980861537595	24.992617912077094	25.39394304479489
58-59	24.24870477911898	25.020524441422015	25.533495664701096	25.19727511475791
60-61	24.41972714396925	25.180317883571373	25.281446096697042	25.118508875762334
62-63	24.023100957715688	25.20085049502423	25.214432338272903	25.561616208987175
64-65	23.891159865912602	25.00256555978957	25.411473247293237	25.69480132700459
66-67	24.478984764444068	24.90013274622095	25.250104050651206	25.37077843868378
68-69	24.359625645645337	24.608526239482654	25.33764343451981	25.694204680352208
70-71	23.862288990208903	24.746322475825846	25.41815277207543	25.973235761889825
72-73	24.348330523832633	24.761145170567563	25.35210502434117	25.53841928125863
74-75	24.458568600937543	24.657825198583502	25.30470508577489	25.578901114704067
76-77	24.14188324943256	24.789746076663548	25.472126594960205	25.59624407894369
78-79	24.202465642023522	24.85621602136428	25.3880700392565	25.553248297355697
80-81	23.831945715738165	24.77603060276619	25.653512881151435	25.738510800344212
82-83	23.960860154008724	24.760967466137497	25.353217448431238	25.92495493142254
84-85	23.976315384118816	24.885424596105924	25.04748475790004	26.090775261875226
86-87	23.914951601054145	24.577064999718367	25.35475182931677	26.153231569910716
88-89	23.907683760189368	24.26320315718258	25.5140064983147	26.315106584313348
90-91	24.082121941012346	24.455306889871892	25.333688496469485	26.128882672646274
92-93	23.872969307815044	24.40389173199699	25.391297824174412	26.331841136013555
94-95	23.400859956572337	24.45264414515111	25.73874404922931	26.407751849047244
96-97	24.005088795055556	24.262552551552073	25.385748226004374	26.346610427388
98-99	23.75620447152989	24.276475058414942	25.34301438155464	26.624306088500525
100	23.675878108025557	24.30851072754229	25.395266556502104	26.62034460793004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	840.0
1	857.0
2	1239.0
3	1663.5
4	1438.0
5	1223.5
6	1607.5
7	2970.5
8	4931.5
9	5036.0
10	3423.5
11	2435.5
12	2269.5
13	2429.5
14	2752.0
15	3276.5
16	3813.5
17	4337.0
18	5152.5
19	6113.0
20	7536.5
21	9430.5
22	11849.5
23	15091.0
24	18889.0
25	23848.0
26	31206.0
27	39416.0
28	47096.5
29	57542.0
30	69618.0
31	81677.5
32	95741.0
33	111560.5
34	128497.0
35	142733.5
36	157576.5
37	179989.5
38	200246.0
39	218566.0
40	233491.0
41	247867.0
42	268425.0
43	300308.5
44	340492.0
45	384542.5
46	435263.0
47	479858.0
48	524105.0
49	567499.0
50	622486.0
51	635651.5
52	598342.0
53	582563.5
54	569941.5
55	540900.0
56	508159.5
57	467378.5
58	429527.5
59	391446.5
60	336795.0
61	278119.5
62	226638.0
63	180967.0
64	139735.0
65	109115.0
66	85011.5
67	65337.0
68	50789.0
69	40561.0
70	34265.0
71	28910.5
72	23873.0
73	21819.0
74	17611.5
75	13184.5
76	9797.0
77	6667.5
78	4672.5
79	3001.5
80	1967.5
81	1396.0
82	997.5
83	656.5
84	420.0
85	250.0
86	162.5
87	113.0
88	66.5
89	42.5
90	27.0
91	21.0
92	15.5
93	9.5
94	6.0
95	5.0
96	4.5
97	2.5
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04895027348878115
2	0.02432733520047961
3	0.021443207776054934
4	0.022330017039520696
5	0.015435274747890512
6	0.024263421019328928
7	0.04912603748694554
8	0.006415385933000042
9	0.004026593412493177
10-11	0.0022290070676301518
12-13	0.0013621709857739815
14-15	0.002236996340273987
16-17	0.006878763746342511
18-19	0.005025252492972636
20-21	0.008464634366143892
22-23	0.01831141289967135
24-25	0.025397897734753594
26-27	0.02840985352147964
28-29	0.045199309982500296
30-31	0.04270665691762357
32-33	0.040461671304705744
34-35	0.04834308876784963
36-37	0.045618746796301665
38-39	0.05509402415189077
40-41	0.05264930672287706
42-43	0.05769053776113737
44-45	0.058101985302294906
46-47	0.06253203698330177
48-49	0.06514452913783604
50-51	0.03391446237308241
52-53	2.9160845150000194E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.872115393219217E-4
64-65	1.7975863448630255E-4
66-67	3.475333600068516E-4
68-69	0.0011224928064589115
70-71	5.592490850684969E-5
72-73	2.2769427034931657E-4
74-75	7.190345379452102E-5
76-77	1.1184981701369938E-4
78-79	2.7962454253424844E-5
80-81	5.592490850684969E-5
82-83	2.0372645241780954E-4
84-85	0.001086541079561651
86-87	0.003986647049273999
88-89	0.002460695974301386
90-91	0.0037549581426027647
92-93	0.0036311244166233117
94-95	0.002700374153616456
96-97	0.0021171572506164522
98-99	0.004062545139390437
100	0.002852170333849334
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2516784E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48690525463687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62824856982712	29.809799239114433
2	12.942110449000452	10.479719990874543
3	4.944173124582592	6.005228065724921
4	2.425472800923089	3.927995499546872
5	1.4318893821376977	2.898638487486475
6	0.8895313823372494	2.160862367862863
7	0.6237376067490747	1.767724377174262
8	0.4340458643549927	1.4058539029046044
9	0.34416430084721955	1.2540732696386745
>10	2.0244899405703185	15.350533514203747
>50	0.1744867373523943	4.90664923344432
>100	0.11610324386017568	9.331915765943727
>500	0.013585048634424807	3.797284694918728
>1k	0.0076561201765393445	5.4546488166725995
>5k	2.036190978498294E-4	0.6069576619073649
>10k+	1.018095489249147E-4	0.842115112581831
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	31066	0.2481947439533989	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	30931	0.24711619214648106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17237	0.13771109256179542	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	12824	0.10245443238454863	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.994636321917835E-5	0.0
3	0.0	7.989272643835669E-6	0.0	4.793563586301401E-5	0.0
4	0.0	7.989272643835669E-6	0.0	7.98927264383567E-5	0.0
5	1.5978545287671338E-5	1.5978545287671338E-5	0.0	9.587127172602802E-5	0.0
6	2.3967817931507005E-5	1.5978545287671338E-5	0.0	9.587127172602802E-5	0.0
7	2.3967817931507005E-5	1.5978545287671338E-5	0.0	1.0386054436986369E-4	1.5978545287671338E-5
8	3.1957090575342676E-5	1.5978545287671338E-5	0.0	1.0386054436986369E-4	1.5978545287671338E-5
9	3.1957090575342676E-5	1.5978545287671338E-5	0.0	1.4380690758904205E-4	2.3967817931507005E-5
10-11	3.1957090575342676E-5	1.5978545287671338E-5	0.0	1.4780154391095987E-4	2.3967817931507005E-5
12-13	3.1957090575342676E-5	1.997318160958917E-5	0.0	2.1571036138356306E-4	2.3967817931507005E-5
14-15	3.595172689726051E-5	2.796245425342484E-5	0.0	2.1571036138356306E-4	3.595172689726051E-5
16-17	3.994636321917835E-5	3.1957090575342676E-5	0.0	2.995977241438376E-4	3.994636321917835E-5
18-19	3.994636321917835E-5	3.1957090575342676E-5	0.0	3.834850869041121E-4	3.994636321917835E-5
20-21	3.994636321917835E-5	3.1957090575342676E-5	0.0	4.513939043767153E-4	3.994636321917835E-5
22-23	4.3940999541096175E-5	3.1957090575342676E-5	0.0	0.0012583104414041178	3.994636321917835E-5
24-25	5.1930272184931846E-5	3.1957090575342676E-5	0.0	0.002999971877760294	3.994636321917835E-5
26-27	7.98927264383567E-5	3.1957090575342676E-5	0.0	0.004865467040095922	3.994636321917835E-5
28-29	9.18766354041102E-5	3.1957090575342676E-5	0.0	0.014400663940513793	4.3940999541096175E-5
30-31	1.1184981701369936E-4	3.1957090575342676E-5	0.0	0.043553519817870145	4.793563586301401E-5
32-33	1.3182299862328853E-4	3.1957090575342676E-5	0.0	0.09711360362214447	4.793563586301401E-5
34-35	1.3581763494520637E-4	3.595172689726051E-5	0.0	0.16944847813943262	4.793563586301401E-5
36-37	1.4380690758904205E-4	4.793563586301401E-5	0.0	0.2813182683347416	5.1930272184931846E-5
38-39	1.5179618023287772E-4	4.793563586301401E-5	0.0	0.4482421363187221	5.592490850684968E-5
40-41	1.5179618023287772E-4	4.793563586301401E-5	0.0	0.642521273835196	5.991954482876752E-5
42-43	1.5579081655479557E-4	4.793563586301401E-5	0.0	0.8238897467592314	6.391418115068535E-5
44-45	1.7975863448630255E-4	5.592490850684968E-5	0.0	1.0382419317933425	7.190345379452102E-5
46-47	1.837532708082204E-4	5.592490850684968E-5	0.0	1.2820625489742414	9.986590804794586E-5
48-49	1.837532708082204E-4	5.592490850684968E-5	0.0	1.534347800521284	1.1184981701369936E-4
50-51	1.837532708082204E-4	5.592490850684968E-5	0.0	1.8068858582204501	1.1184981701369936E-4
52-53	1.837532708082204E-4	5.592490850684968E-5	0.0	2.0729925514413288	1.1983908965753503E-4
54-55	1.957371797739739E-4	9.18766354041102E-5	0.0	2.3462696168600496	1.278283623013707E-4
56-57	1.9973181609589171E-4	9.587127172602802E-5	0.0	2.6567367464358256	1.278283623013707E-4
58-59	1.9973181609589171E-4	1.1184981701369936E-4	0.0	2.9921423905693345	1.3182299862328853E-4
60-61	1.9973181609589171E-4	1.1184981701369936E-4	0.0	3.3299488111323163	1.3581763494520637E-4
62-63	2.0772108873972738E-4	1.158444533356172E-4	0.0	3.6764355764228256	1.4380690758904205E-4
64-65	2.0772108873972738E-4	1.1983908965753503E-4	0.0	4.042156515603369	1.4380690758904205E-4
66-67	2.0772108873972738E-4	1.1983908965753503E-4	0.0	4.440533606715591	1.4380690758904205E-4
68-69	2.0772108873972738E-4	1.2383372597945288E-4	0.0	4.866721355901005	1.4380690758904205E-4
70-71	2.0772108873972738E-4	1.3981227126712422E-4	0.0	5.338499889428467	1.4380690758904205E-4
72-73	2.0772108873972738E-4	1.5179618023287772E-4	7.989272643835669E-6	5.802013520405881	1.4380690758904205E-4
74-75	2.1171572506164523E-4	1.5179618023287772E-4	7.989272643835669E-6	6.2677921101778225	1.4380690758904205E-4
76-77	2.1571036138356306E-4	1.5179618023287772E-4	7.989272643835669E-6	6.7694984590290925	1.4380690758904205E-4
78-79	2.1571036138356306E-4	1.597854528767134E-4	7.989272643835669E-6	7.294996861813706	1.4380690758904205E-4
80-81	2.1571036138356306E-4	1.637800891986312E-4	7.989272643835669E-6	7.849572222385559	1.5179618023287772E-4
82-83	2.1571036138356306E-4	1.6777472552054906E-4	7.989272643835669E-6	8.432928937656829	1.597854528767134E-4
84-85	2.1571036138356306E-4	1.717693618424669E-4	7.989272643835669E-6	9.014571953945998	1.597854528767134E-4
86-87	2.1571036138356306E-4	1.837532708082204E-4	7.989272643835669E-6	9.608586358924146	1.6777472552054906E-4
88	2.1571036138356306E-4	1.837532708082204E-4	7.989272643835669E-6	10.082238376886586	1.6777472552054906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4295	0.0	18.827066	1
GTATAAG	4895	0.0	17.959997	1
CATGGGG	28440	0.0	17.601467	4
GTACATG	85985	0.0	17.501705	1
TACATGG	86250	0.0	16.889927	2
TAGTACT	5030	0.0	16.820286	4
GTACAAG	7040	0.0	15.760018	1
TAGGCAT	6130	0.0	15.7165165	5
GTATCAA	61695	0.0	15.697651	1
GTATAAA	15280	0.0	15.69149	1
ACATGGG	85045	0.0	15.60282	3
GTACATA	3555	0.0	15.340367	1
GTATTAG	4150	0.0	14.953513	1
CTAGGCA	6625	0.0	14.828228	4
GTATATA	3815	0.0	14.541353	1
GTCCTAC	4320	0.0	14.365064	1
GTATTAC	2200	0.0	14.317576	1
GTCCTAT	4850	0.0	14.152348	1
TAGGACT	5620	0.0	14.1344595	4
TACACTA	4690	0.0	14.028722	5
>>END_MODULE