##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765253_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7230796 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19898998671792 33.0 31.0 34.0 31.0 34.0 2 32.24914158828433 34.0 31.0 34.0 31.0 34.0 3 32.271013869012485 34.0 31.0 34.0 30.0 34.0 4 35.739501294186695 37.0 35.0 37.0 35.0 37.0 5 35.75297076559759 37.0 35.0 37.0 35.0 37.0 6 35.852288323443226 37.0 37.0 37.0 35.0 37.0 7 35.80264579446025 37.0 36.0 37.0 35.0 37.0 8 35.836509147817196 37.0 37.0 37.0 35.0 37.0 9 37.531539404513694 39.0 38.0 39.0 35.0 39.0 10-11 37.45778141161775 39.0 38.0 39.0 35.0 39.0 12-13 37.44258910084035 39.0 38.0 39.0 35.0 39.0 14-15 38.81168062271429 41.0 38.5 41.0 35.0 41.0 16-17 38.75122068165109 40.0 38.0 41.0 35.0 41.0 18-19 38.740589486966584 40.0 38.5 41.0 35.0 41.0 20-21 38.71821421873885 40.5 39.0 41.0 35.0 41.0 22-23 38.61572273647327 40.0 38.5 41.0 34.5 41.0 24-25 38.53318265374932 40.0 38.0 41.0 34.0 41.0 26-27 38.3909243878544 40.0 38.0 41.0 34.0 41.0 28-29 38.23186292352875 40.0 38.0 41.0 34.0 41.0 30-31 37.994739445007156 40.0 38.0 41.0 34.0 41.0 32-33 37.713234061644116 40.0 37.0 41.0 33.0 41.0 34-35 37.52735480298435 40.0 37.0 41.0 33.0 41.0 36-37 37.35764464935811 40.0 37.0 41.0 33.0 41.0 38-39 37.13522570682398 40.0 37.0 41.0 32.0 41.0 40-41 36.85285112455116 40.0 36.0 41.0 31.0 41.0 42-43 36.55046747273744 40.0 36.0 41.0 30.5 41.0 44-45 36.16035419890147 39.5 35.0 41.0 30.0 41.0 46-47 35.68515001667866 39.0 35.0 41.0 28.0 41.0 48-49 35.263990506716 39.0 35.0 41.0 26.5 41.0 50-51 33.98556936193471 38.0 34.0 40.0 24.5 40.5 52-53 33.918114893574646 38.0 33.5 39.5 23.0 40.0 54-55 34.710555241774216 39.0 35.0 40.5 24.0 41.0 56-57 34.707117169396014 39.0 35.0 41.0 24.0 41.0 58-59 34.487706540192804 38.0 35.0 41.0 23.0 41.0 60-61 34.13836892922993 37.5 35.0 40.0 22.5 41.0 62-63 33.739318534225 37.0 34.0 40.0 22.0 41.0 64-65 33.409539627449035 36.5 34.0 39.5 20.0 41.0 66-67 33.06327553702248 36.0 34.0 39.0 19.0 41.0 68-69 32.79553081016253 35.5 34.0 39.0 17.5 41.0 70-71 32.41879925806232 35.0 34.0 38.5 14.0 40.5 72-73 32.0411365498349 35.0 33.5 37.0 9.5 39.5 74-75 31.679859105415225 35.0 33.0 37.0 6.5 39.0 76-77 31.310232096162025 35.0 33.0 36.0 2.0 39.0 78-79 30.975033039239385 35.0 33.0 36.0 2.0 37.0 80-81 30.683325736198338 35.0 32.5 35.0 2.0 37.0 82-83 30.458285505496214 35.0 32.0 35.0 2.0 36.5 84-85 30.22201138021319 35.0 32.0 35.0 2.0 36.0 86-87 29.978670882154606 35.0 32.0 35.0 2.0 36.0 88-89 29.756592220275607 35.0 32.0 35.0 2.0 35.5 90-91 29.50986661772784 35.0 31.0 35.0 2.0 35.0 92-93 29.2200405045309 35.0 31.0 35.0 2.0 35.0 94-95 28.913868611422586 35.0 31.0 35.0 2.0 35.0 96-97 28.629351111551202 35.0 31.0 35.0 2.0 35.0 98-99 28.32083072181818 35.0 30.0 35.0 2.0 35.0 100 27.765465932104846 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4484377638159387 1101 2 0.5503476657252335 1101 3 0.552005974565823 1101 4 0.49621965890585074 1101 5 0.4837867664616766 1101 6 0.4302152525739018 1101 7 0.4340990190519207 1101 8 0.36099353521795763 1101 9 0.4890707993897436 1101 10-11 0.3955412969924126 1101 12-13 0.5026675181812976 1101 14-15 0.6604085518494927 1101 16-17 0.6092713283779219 1101 18-19 0.5659653443751083 1101 20-21 0.5614302287769917 1101 22-23 0.6795853721160157 1101 24-25 0.6191206006281718 1101 26-27 0.7028583379349413 1101 28-29 0.7187462095003738 1101 30-31 0.6604939885204502 1101 32-33 0.6883686203768349 1101 34-35 0.7442093965720815 1101 36-37 0.7651892120502524 1101 38-39 0.7571769987207162 1101 40-41 0.8082658195909289 1101 42-43 0.8276303090534967 1101 44-45 0.8142608030145126 1101 46-47 0.8849116989339478 1101 48-49 0.9472038591303544 1101 50-51 0.9174640384386734 1101 52-53 0.9782149058419307 1101 54-55 1.0501187425763092 1101 56-57 1.109508244791492 1101 58-59 1.084663407144113 1101 60-61 1.1027673113760343 1101 62-63 1.109815351959682 1101 64-65 1.083657467930685 1101 66-67 1.055300590981517 1101 68-69 0.9853850110709956 1101 70-71 0.9709364361139734 1101 72-73 0.967677902736277 1101 74-75 0.8587380964529672 1101 76-77 0.9431154491248037 1101 78-79 0.8825284831279667 1101 80-81 0.8985718619086889 1101 82-83 0.8941004415275948 1101 84-85 0.9907022092484361 1101 86-87 0.9312738749113159 1101 88-89 0.9225144753908197 1101 90-91 0.9047706276116152 1101 92-93 0.8928322192356752 1101 94-95 0.785645283312622 1101 96-97 0.7631672617810885 1101 98-99 0.8047566876389567 1101 100 0.9027063913087758 1102 1 0.5495523083266178 1102 2 0.6008198677623255 1102 3 0.6392094698985815 1102 4 0.5339703495842514 1102 5 0.48286651228508504 1102 6 0.5007345432982078 1102 7 0.586218949379969 1102 8 0.487501241795691 1102 9 0.5220988916027522 1102 10-11 0.5945712110702317 1102 12-13 0.5998230005982634 1102 14-15 0.7890369619566755 1102 16-17 0.7971447412917456 1102 18-19 0.7763406158627362 1102 20-21 0.7816569711682675 1102 22-23 0.8350619358760767 1102 24-25 0.7817470371207307 1102 26-27 0.8350029355551598 1102 28-29 0.7303812825801899 1102 30-31 0.7008665906567373 1102 32-33 0.7677560793971594 1102 34-35 0.7546439299717775 1102 36-37 0.7255268893301619 1102 38-39 0.836747785958238 1102 40-41 0.7980074424235823 1102 42-43 0.7636410473032953 1102 44-45 0.7036429088798002 1102 46-47 0.6383334583240838 1102 48-49 0.6498450211349791 1102 50-51 0.6489545907848395 1102 52-53 0.6364491971356543 1102 54-55 0.6818530014967408 1102 56-57 0.6601102109775923 1102 58-59 0.717005785343936 1102 60-61 0.6584642592105254 1102 62-63 0.6653184213524099 1102 64-65 0.6456891933230793 1102 66-67 0.5401985226172172 1102 68-69 0.5313614986671169 1102 70-71 0.4880918599988746 1102 72-73 0.5221823319517753 1102 74-75 0.4894453163040424 1102 76-77 0.42748390039808726 1102 78-79 0.38705089830849815 1102 80-81 0.41545818280547664 1102 82-83 0.47626931189047994 1102 84-85 0.4677725968781381 1102 86-87 0.4068207667573489 1102 88-89 0.30926966566773473 1102 90-91 0.33926323551729 1102 92-93 0.44438807747187425 1102 94-95 0.4049783132908651 1102 96-97 0.5167896199615036 1102 98-99 0.4838983625579232 1102 100 0.4414502483321314 1103 1 0.5420720907202821 1103 2 0.6035207613807998 1103 3 0.673371404139175 1103 4 0.52100414166447 1103 5 0.5346951046654524 1103 6 0.5048061350987467 1103 7 0.5176302142593983 1103 8 0.4980996378869591 1103 9 0.5977121884409797 1103 10-11 0.6372067460475463 1103 12-13 0.6089513069013464 1103 14-15 0.7649618101779581 1103 16-17 0.8013069313391554 1103 18-19 0.7658256234516685 1103 20-21 0.7068358760592872 1103 22-23 0.748374335685078 1103 24-25 0.7363202767435055 1103 26-27 0.6988782702357383 1103 28-29 0.7284155670885255 1103 30-31 0.7606958076320822 1103 32-33 0.8310072454498965 1103 34-35 0.7601616296716358 1103 36-37 0.8111875317463344 1103 38-39 0.7823587272420482 1103 40-41 0.8391353159868018 1103 42-43 0.8323576858312123 1103 44-45 0.8327243599013272 1103 46-47 0.8133404930114807 1103 48-49 0.8450432074646557 1103 50-51 0.7943437262430777 1103 52-53 0.8494448761322033 1103 54-55 0.9416599952550584 1103 56-57 0.8987731727088928 1103 58-59 0.9290435211125541 1103 60-61 0.8941630594765613 1103 62-63 0.9187355740520289 1103 64-65 0.8430406868085285 1103 66-67 0.8189210979949593 1103 68-69 0.8032516339036491 1103 70-71 0.7836190778439587 1103 72-73 0.747512429328907 1103 74-75 0.7610673873160181 1103 76-77 0.7737578609697309 1103 78-79 0.8260404400732249 1103 80-81 0.7538450330561624 1103 82-83 0.7011440198443673 1103 84-85 0.7152784804348791 1103 86-87 0.7451969583907605 1103 88-89 0.7553288018081226 1103 90-91 0.7270282620359296 1103 92-93 0.7149117779732599 1103 94-95 0.6353494037100482 1103 96-97 0.6809373888997321 1103 98-99 0.6179507262128894 1103 100 0.6005956617159462 1104 1 0.4489960166839424 1104 2 0.5352933970955434 1104 3 0.5514815642272382 1104 4 0.5058050343281764 1104 5 0.556299893839892 1104 6 0.49601783312135694 1104 7 0.5017969797368238 1104 8 0.5147524527492706 1104 9 0.5476061681576923 1104 10-11 0.6140076270003476 1104 12-13 0.6056521712392993 1104 14-15 0.7145702451033884 1104 16-17 0.7091320480090388 1104 18-19 0.7016263986403644 1104 20-21 0.7051129549644344 1104 22-23 0.6984414172105389 1104 24-25 0.721731646055666 1104 26-27 0.7577800490489608 1104 28-29 0.7944050049504696 1104 30-31 0.79644796426836 1104 32-33 0.8137612458217092 1104 34-35 0.8101495802486838 1104 36-37 0.7117510641357754 1104 38-39 0.7714887662247207 1104 40-41 0.7854044962527524 1104 42-43 0.8380081058135289 1104 44-45 0.8610141085413971 1104 46-47 0.8174180836099652 1104 48-49 0.7508356587619289 1104 50-51 0.7280810949345593 1104 52-53 0.7436228090550756 1104 54-55 0.7878092668367955 1104 56-57 0.8218732431207059 1104 58-59 0.7830439478286451 1104 60-61 0.8363376317357663 1104 62-63 0.7940660411414555 1104 64-65 0.7965569499613423 1104 66-67 0.7443912084726989 1104 68-69 0.7664714336764575 1104 70-71 0.7090703868303194 1104 72-73 0.7137110074787003 1104 74-75 0.7125540500595307 1104 76-77 0.685939617136988 1104 78-79 0.7337860506797895 1104 80-81 0.7949591371573241 1104 82-83 0.7546691210704992 1104 84-85 0.7336689922599611 1104 86-87 0.728750502476462 1104 88-89 0.7530527234533864 1104 90-91 0.7685257152801448 1104 92-93 0.7754894512800057 1104 94-95 0.7409142425972775 1104 96-97 0.7466909618235356 1104 98-99 0.7192727409569173 1104 100 0.6891563586026024 1105 1 0.35081884431121324 1105 2 0.35337808844154495 1105 3 0.43564213363767834 1105 4 0.38540767739461756 1105 5 0.34439213542340497 1105 6 0.3396050359345395 1105 7 0.31989068935457254 1105 8 0.2977702964259521 1105 9 0.36330369057113643 1105 10-11 0.43026467377393374 1105 12-13 0.47064027292576327 1105 14-15 0.5497209762003692 1105 16-17 0.5652102322129622 1105 18-19 0.48961922322814644 1105 20-21 0.5023375062318394 1105 22-23 0.5369793598743655 1105 24-25 0.5349359808418797 1105 26-27 0.5373913080750299 1105 28-29 0.6040172864973457 1105 30-31 0.6013695294698849 1105 32-33 0.5952576476527867 1105 34-35 0.6066461895810136 1105 36-37 0.6641220879318368 1105 38-39 0.6513682486331618 1105 40-41 0.6975592392116283 1105 42-43 0.6523685479367316 1105 44-45 0.6630885054491955 1105 46-47 0.6425023848752147 1105 48-49 0.6395551940476878 1105 50-51 0.6497337790948947 1105 52-53 0.5879532362638358 1105 54-55 0.6341127109984726 1105 56-57 0.6624914499691315 1105 58-59 0.6754185832535313 1105 60-61 0.6692150968324242 1105 62-63 0.6621571978322791 1105 64-65 0.751846991216155 1105 66-67 0.6863712121561463 1105 68-69 0.5921645357867007 1105 70-71 0.5870909307233703 1105 72-73 0.6258190660308287 1105 74-75 0.6556525894478114 1105 76-77 0.5959358610538033 1105 78-79 0.622396894190338 1105 80-81 0.6481247705973558 1105 82-83 0.5935306279842898 1105 84-85 0.5341919089170126 1105 86-87 0.6025919144397278 1105 88-89 0.6724688222186899 1105 90-91 0.7062472316425961 1105 92-93 0.6903054079292268 1105 94-95 0.6269784773708658 1105 96-97 0.5731383451625369 1105 98-99 0.6407292777067397 1105 100 0.6965883055494579 1106 1 0.30559088961001635 1106 2 0.2968729545120894 1106 3 0.34544971254364043 1106 4 0.30302223793427885 1106 5 0.2783859910542006 1106 6 0.2909205816604725 1106 7 0.27902551845249235 1106 8 0.29493483000891985 1106 9 0.3902359752795803 1106 10-11 0.3069513991751833 1106 12-13 0.39596696412287713 1106 14-15 0.47870887896944936 1106 16-17 0.49850335820142533 1106 18-19 0.45465887466766475 1106 20-21 0.4642498078377244 1106 22-23 0.4790517983352842 1106 24-25 0.47527112978499986 1106 26-27 0.4191752984580077 1106 28-29 0.45709106599218075 1106 30-31 0.5222597106966376 1106 32-33 0.5234141238379095 1106 34-35 0.4931933721037822 1106 36-37 0.44520388016751156 1106 38-39 0.5272656165065399 1106 40-41 0.563511795186642 1106 42-43 0.5166936393060269 1106 44-45 0.5448385035533789 1106 46-47 0.5728245568084986 1106 48-49 0.42993880967301834 1106 50-51 0.4209910083221331 1106 52-53 0.5276257403982143 1106 54-55 0.5452771283047326 1106 56-57 0.4799284658514935 1106 58-59 0.4108899595038835 1106 60-61 0.4826237508284592 1106 62-63 0.4793448086096177 1106 64-65 0.48026340481648333 1106 66-67 0.46490459458409816 1106 68-69 0.5096339189628623 1106 70-71 0.48957068190566844 1106 72-73 0.4140233721447828 1106 74-75 0.46648631628831794 1106 76-77 0.47734307158168576 1106 78-79 0.4899643602573427 1106 80-81 0.5220749610756918 1106 82-83 0.5063555753317637 1106 84-85 0.49322230564566283 1106 86-87 0.45709852144818086 1106 88-89 0.41501064060579296 1106 90-91 0.47358579148940905 1106 92-93 0.48309002916749577 1106 94-95 0.3227688634540584 1106 96-97 0.3469444656745573 1106 98-99 0.43003078572893116 1106 100 0.49328561381597424 1107 1 0.24916689862742203 1107 2 0.3023217825152855 1107 3 0.30994986878634734 1107 4 0.27980681039785793 1107 5 0.3435634784706849 1107 6 0.2786083914090298 1107 7 0.2834732018523738 1107 8 0.29251831286573093 1107 9 0.3706608650024563 1107 10-11 0.3716362560302784 1107 12-13 0.3800680633590048 1107 14-15 0.3915719612713602 1107 16-17 0.42674494770544413 1107 18-19 0.4387672317719833 1107 20-21 0.4300637154353524 1107 22-23 0.44873371950402685 1107 24-25 0.43741821456517016 1107 26-27 0.4165341844877446 1107 28-29 0.490277690380843 1107 30-31 0.4825643779139952 1107 32-33 0.47140778103468506 1107 34-35 0.5041739972356609 1107 36-37 0.5887098915685414 1107 38-39 0.5268754233645154 1107 40-41 0.5130327593403763 1107 42-43 0.5445429619914037 1107 44-45 0.5050733716978826 1107 46-47 0.4883274294572928 1107 48-49 0.5545731173512536 1107 50-51 0.5950032185272036 1107 52-53 0.5685551313781403 1107 54-55 0.5411805690968023 1107 56-57 0.5619861884052568 1107 58-59 0.4799656060139341 1107 60-61 0.503842823403069 1107 62-63 0.5123988500564494 1107 64-65 0.5366839984990719 1107 66-67 0.5500094967004898 1107 68-69 0.526315720895191 1107 70-71 0.47691445987755543 1107 72-73 0.5086305554709227 1107 74-75 0.49308694370785844 1107 76-77 0.5017035877639202 1107 78-79 0.459375181297613 1107 80-81 0.5168752610528706 1107 82-83 0.48529837086625705 1107 84-85 0.47828174129898215 1107 86-87 0.5357716066587521 1107 88-89 0.5541668284954575 1107 90-91 0.527129831693145 1107 92-93 0.456621366998867 1107 94-95 0.3686076948799233 1107 96-97 0.3625848407320653 1107 98-99 0.4751667069592216 1107 100 0.49492289181537785 1108 1 0.19258037827252394 1108 2 0.23559305017653287 1108 3 0.30465026525446604 1108 4 0.2852963137042437 1108 5 0.2584261263570298 1108 6 0.2340186435789704 1108 7 0.2991555940279582 1108 8 0.2267164810800324 1108 9 0.32158675673860415 1108 10-11 0.3164403943839602 1108 12-13 0.3581177676340346 1108 14-15 0.35506650053421396 1108 16-17 0.3992044517119453 1108 18-19 0.39854889382890946 1108 20-21 0.4017380766484919 1108 22-23 0.3484497975843581 1108 24-25 0.42408334854169993 1108 26-27 0.43143478975120075 1108 28-29 0.41287714284470667 1108 30-31 0.48491415158290607 1108 32-33 0.44062254813539425 1108 34-35 0.42396747117351197 1108 36-37 0.47165677482412605 1108 38-39 0.42854613292732324 1108 40-41 0.3938087876834828 1108 42-43 0.3959812403467424 1108 44-45 0.38629144246354485 1108 46-47 0.4733223505824853 1108 48-49 0.37018174992570607 1108 50-51 0.3907276698493476 1108 52-53 0.4056677479993809 1108 54-55 0.3710012149994597 1108 56-57 0.417798360204948 1108 58-59 0.4106004133330927 1108 60-61 0.37471603621927585 1108 62-63 0.38179304288768634 1108 64-65 0.3844834445508454 1108 66-67 0.40485247563354676 1108 68-69 0.3832708216879084 1108 70-71 0.39019066879168207 1108 72-73 0.4028501721792068 1108 74-75 0.4324503467906382 1108 76-77 0.41997938237553 1108 78-79 0.38893573716865504 1108 80-81 0.40405055465707207 1108 82-83 0.4458619019363894 1108 84-85 0.4119392534432187 1108 86-87 0.4388248982373959 1108 88-89 0.4354345704083613 1108 90-91 0.4479066301442778 1108 92-93 0.45397615263020086 1108 94-95 0.439049242848796 1108 96-97 0.3962220184938303 1108 98-99 0.42520441905938 1108 100 0.4777691253169394 1109 1 0.21707209072028633 1109 2 0.283072133653846 1109 3 0.3083593820889732 1109 4 0.3063439844981559 1109 5 0.32357676077617015 1109 6 0.322191779011483 1109 7 0.3487311115148515 1109 8 0.23906682648654254 1109 9 0.3161009404935271 1109 10-11 0.2368862557824798 1109 12-13 0.2967711374197535 1109 14-15 0.3345218324627339 1109 16-17 0.39229068691034996 1109 18-19 0.3168980049904917 1109 20-21 0.3479708602253666 1109 22-23 0.39162292822553724 1109 24-25 0.42130788816738374 1109 26-27 0.3960514024196513 1109 28-29 0.29752219680938197 1109 30-31 0.3386555044418387 1109 32-33 0.38619556646796127 1109 34-35 0.29299098986398775 1109 36-37 0.3274793157013036 1109 38-39 0.33796195560235276 1109 40-41 0.25374723834195123 1109 42-43 0.2001064836261932 1109 44-45 0.16516622889029975 1109 46-47 0.15240805106918742 1109 48-49 0.1823765114759155 1109 50-51 0.08618038763176372 1109 52-53 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-0.705661969229535 2316 94-95 -0.5641505860114933 2316 96-97 -0.788156379638199 2316 98-99 -0.6089540189107154 2316 100 -0.6568934247640641 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21138.0 3 3108.0 4 3086.0 5 3503.0 6 4566.0 7 5943.0 8 7862.0 9 9697.0 10 12738.0 11 18617.0 12 28507.0 13 39641.0 14 41587.0 15 50971.0 16 65737.0 17 86643.0 18 84722.0 19 48837.0 20 41207.0 21 38706.0 22 39977.0 23 45587.0 24 50891.0 25 54280.0 26 58811.0 27 67008.0 28 77649.0 29 91820.0 30 110436.0 31 135875.0 32 174119.0 33 234652.0 34 339884.0 35 548279.0 36 995408.0 37 1756015.0 38 1543045.0 39 289812.0 40 432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.389064297292094 18.01344331232585 13.341047919484083 24.25644447089797 2 15.634710947825955 21.83708283578053 36.15749158494319 26.370714631450333 3 19.138948296953405 26.400134812386096 29.532497829409625 24.92841906125087 4 13.530640142091974 18.398288904162126 33.843217514874645 34.22785343887125 5 13.968979221250589 35.821518417616176 33.307698198945666 16.90180416218757 6 31.26782588361246 33.66859524694823 17.884428150363874 17.179150719075434 7 25.51573931872862 32.07098530568314 21.844252323473384 20.56902305211486 8 27.30718983579909 30.530069703804543 21.323859799005277 20.838880661391094 9 27.418366883113283 14.272865753947093 19.777467785226715 38.53129957771291 10-11 24.673224900830864 25.35094883291043 26.376835519642405 23.598990746616302 12-13 26.86055202031706 23.748168127843638 26.542609301300978 22.848670550538323 14-15 25.321387820472633 23.565482489190792 25.109612476888373 26.003517213448195 16-17 22.622861305509026 26.58168057591187 27.232678685285094 23.562779433294008 18-19 23.82753992895823 25.223659384093146 29.058935520566827 21.8898651663818 20-21 24.280398579288143 24.644688645703937 28.273280191183687 22.801632583824237 22-23 23.088900993736573 25.37015066471684 26.851903160972245 24.689045180574343 24-25 22.838524163151774 26.399800237135263 26.350857226711938 24.410818373001028 26-27 23.739940220363362 25.071641406983815 26.69034471085458 24.49807366179824 28-29 22.92041387239731 24.501096223357198 28.982849570849435 23.595640333396055 30-31 24.128517206904018 24.88734323743634 28.029593202997503 22.954546352662135 32-33 21.886504977645764 25.62787406777649 28.104786749897375 24.380834204680372 34-35 21.71365731604558 26.128893011242198 28.449808243855557 23.70764142885666 36-37 23.1371204472699 25.89607611854004 27.19514714321754 23.77165629097252 38-39 23.819824285341443 24.670747891486403 27.10085351273554 24.408574310436613 40-41 22.849857918076 25.839562576927438 27.284865691377334 24.025713813619223 42-43 24.32499064318789 25.311544111467015 26.983744555359994 23.379720689985103 44-45 24.014356002806554 25.11622602644541 26.9207666053428 23.948651365405237 46-47 22.822985911323926 25.501222217749252 28.156461609807565 23.519330261119258 48-49 23.736089942351683 26.014423581956432 27.17098620226497 23.078500273426922 50-51 22.60304297025585 26.337933779109722 26.451268521013844 24.607754729620588 52-53 22.380588047555726 26.36784759654066 25.401926855762525 25.849637500141085 54-55 23.064418266771558 26.24756829249269 25.19840359427288 25.489609846462873 56-57 23.537848590130967 24.888757589650616 25.52250873426873 26.050885085949695 58-59 23.256618999478633 24.654018924455563 26.920769639491084 25.16859243657472 60-61 23.49350370985148 25.124180954018776 26.39998327361404 24.982332062515695 62-63 21.716622921359267 25.37577292917027 26.95252904751195 25.955075101958514 64-65 21.640638277913755 25.397746802397126 27.067488969217653 25.89412595047147 66-67 23.080198271435414 25.187910557281697 26.113525260832365 25.618365910450525 68-69 23.4848980937847 24.25671392344709 26.144273954297848 26.114114028470357 70-71 22.261577021388614 24.75170299036543 26.673603512588347 26.313116475657605 72-73 23.34498480064231 24.298181527556746 26.691253615097953 25.665580056702993 74-75 23.587270502276954 23.73550865689068 26.803274957458434 25.873945883373928 76-77 22.550371720765895 24.496338207918413 27.81507323099277 25.138216840322926 78-79 22.99301259799889 24.303967195579425 27.583044296180702 25.11997591024098 80-81 22.131640593472333 24.2465963361164 27.57662370995937 26.04513936045189 82-83 22.124541186010575 24.83467461730732 26.292526746332346 26.748257450349755 84-85 22.422580613431116 24.653382784799536 26.114822680306965 26.809213921462387 86-87 22.961068457803087 23.448253979382635 26.553689303499013 27.03698825931527 88-89 22.905096463965734 22.991263657829244 27.45795903304062 26.64568084516441 90-91 23.559522396115533 23.49467317920342 26.7723155215721 26.173488903108954 92-93 22.491850648309892 23.640019061482974 27.076354698390386 26.791775591816748 94-95 22.014149511847325 23.83789737596032 27.460034163087972 26.687918949104382 96-97 23.5111124158455 23.38495570461452 26.57952845372388 26.5244034258161 98-99 23.575652120781214 22.904950896527208 26.667740573688537 26.851656409003045 100 23.044626306360772 23.339599345652726 27.150188702729857 26.465585645256652 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3438.0 1 3864.5 2 2743.0 3 1066.5 4 845.0 5 852.5 6 1112.5 7 1667.0 8 2384.0 9 2477.0 10 1976.5 11 1776.5 12 1952.0 13 2189.0 14 2472.5 15 2781.5 16 3233.5 17 3775.5 18 4447.5 19 5272.5 20 6282.0 21 7670.0 22 9489.0 23 12073.0 24 15532.5 25 19712.0 26 24423.5 27 29807.0 28 36064.0 29 43680.5 30 52207.0 31 60640.5 32 68494.5 33 76315.5 34 84652.5 35 91197.5 36 96752.5 37 103129.0 38 109153.5 39 116464.0 40 122266.0 41 127795.0 42 138838.0 43 157879.5 44 180381.0 45 199923.0 46 226803.5 47 263117.0 48 334861.0 49 445949.5 50 607071.0 51 589798.5 52 408763.0 53 328107.0 54 285975.0 55 250712.5 56 227750.0 57 204368.0 58 187148.0 59 168266.5 60 142388.5 61 116593.0 62 92754.0 63 70834.0 64 51918.5 65 42290.5 66 33353.5 67 23361.0 68 17369.5 69 13404.0 70 10660.5 71 9252.5 72 7804.0 73 7755.0 74 6361.0 75 4950.0 76 3754.0 77 2415.5 78 1708.5 79 1085.5 80 702.0 81 537.0 82 395.5 83 270.5 84 184.5 85 114.5 86 81.5 87 61.5 88 49.0 89 43.5 90 35.5 91 35.5 92 34.0 93 30.0 94 28.5 95 20.0 96 9.5 97 10.5 98 10.5 99 4.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08831669431691891 2 0.28359256712538977 3 0.28732659585473025 4 0.29569358615565977 5 0.32140306544397046 6 0.170935537387585 7 0.18786313429392834 8 0.15356538892813462 9 0.20539923958579387 10-11 0.22617150310975442 12-13 0.2426703781990254 14-15 0.17007117888542284 16-17 0.15900047518973015 18-19 0.17686849414642591 20-21 0.20907103450297865 22-23 0.17126745105241525 24-25 0.1969285262646049 26-27 0.19533810662062653 28-29 0.22478161463827773 30-31 0.2590931897401061 32-33 0.22669011821105173 34-35 0.15322656039528704 36-37 0.1470999873319618 38-39 0.11466234146282096 40-41 0.10613630919749362 42-43 0.12149423106391052 44-45 0.13614683639256314 46-47 0.20205935833343935 48-49 0.20708646738201436 50-51 0.213593358186291 52-53 0.14068298981191008 54-55 0.08562681065819033 56-57 0.12384528618979156 58-59 0.14314468282606785 60-61 0.11995913036407058 62-63 0.14122926438527653 64-65 0.11607988940636688 66-67 0.11999370470415706 68-69 0.10559003462412714 70-71 0.13075323933907138 72-73 0.15554995604909888 74-75 0.1940381114333747 76-77 0.17437914166019897 78-79 0.19986043030393888 80-81 0.10340493633066124 82-83 0.14401595619624727 84-85 0.1752919042384822 86-87 0.1884647278114332 88-89 0.1697116057485234 90-91 0.1730514870008779 92-93 0.12311922504797536 94-95 0.36284386947163216 96-97 0.3632311020806008 98-99 0.4104112465626191 100 0.44493026770496635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7230796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.5661382969054 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23593261858352 31.68833318417604 2 14.084759812309311 11.708981484742884 3 4.245173493551508 5.293664055819571 4 1.7491841599637945 2.908273227992455 5 0.9043491442537139 1.8795150799368963 6 0.5685674663950568 1.4179892363578828 7 0.3721340959639464 1.0827724108481884 8 0.2540656172957686 0.8448421268003646 9 0.20749077009001188 0.7762131040403553 >10 1.139935370572793 8.968360461535887 >50 0.11520030914128404 3.333176611352516 >100 0.09900526209641815 8.463363361179665 >500 0.013456680496772188 3.9071953500002774 >1k 0.009339285361037673 7.186561193169562 >5k 6.360086804554502E-4 1.8771392116444023 >10k+ 7.699052447618607E-4 8.663619900403168 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 89054 1.231593312824757 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 88223 1.2201008021800088 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 39079 0.540452254495909 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 37539 0.5191544610026337 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 33011 0.456533416237991 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 30892 0.4272282055806857 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 30583 0.42295481714599614 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 29632 0.40980273817709695 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 28158 0.389417707262105 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 23792 0.3290370797350665 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22301 0.3084169433074865 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 20793 0.28756170136731835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17973 0.24856184574976256 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 17001 0.23511934232413692 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 15400 0.21297793493275152 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 14879 0.20577264245872792 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 14667 0.20284073841939396 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 12885 0.17819614880574697 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 12861 0.1778642351409167 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 11578 0.16012068380853228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11081 0.15324730499933895 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10764 0.14886327867637256 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 10513 0.1453920149316894 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 9642 0.1333463148455578 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 9350 0.1293080319234563 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8837 0.12221337733770944 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 8831 0.12213039892150186 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 8685 0.12011125746045109 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 8192 0.11329319759539613 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7526 0.10408259339635637 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 7499 0.10370919052342233 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7294 0.1008740946363305 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.521270963805368E-4 0.0 0.0 0.0 0.0 2 2.6276498465729084E-4 0.0 0.0 0.0 0.0 3 3.042541927610736E-4 0.0 0.0 0.0 0.0 4 4.7021102517620466E-4 0.0 0.0 8.297841620756553E-5 0.0 5 0.0021436090853621096 0.0 0.0 2.0744604051891382E-4 0.0 6 0.003941474769859362 0.0 0.0 2.2127577655350807E-4 0.0 7 0.0043840263229663785 0.0 0.0 2.3510551258810233E-4 0.0 8 0.004840407612107989 0.0 0.0 2.489352486226966E-4 0.0 9 0.005144661804869063 0.0 0.0 2.9042445672647935E-4 0.0 10-11 0.008055821240151154 0.0 0.0 3.80317740951342E-4 0.0 12-13 0.016651002185651482 0.0 0.0 4.563812891416104E-4 0.0 14-15 0.026387136354005837 1.3829736034594255E-5 0.0 5.324448373318788E-4 0.0 16-17 0.030079675875242504 1.3829736034594255E-5 0.0 7.60635481902684E-4 0.0 18-19 0.03227168903672569 1.3829736034594255E-5 0.0 0.001023400466559975 1.3829736034594255E-5 20-21 0.04267165053474058 1.3829736034594255E-5 0.0 0.0025239268263134514 2.765947206918851E-5 22-23 0.04686897542123993 1.3829736034594255E-5 0.0 0.009300497483264636 4.1489208103782764E-5 24-25 0.06916942477702318 1.3829736034594255E-5 0.0 0.024202038060539947 5.531894413837702E-5 26-27 0.13982554617776521 1.3829736034594255E-5 0.0 0.04421366610259783 6.914868017297127E-5 28-29 0.15726484331738858 1.3829736034594255E-5 0.0 0.10702832717172495 8.297841620756553E-5 30-31 0.1598164296157712 1.3829736034594255E-5 0.0 0.2735590936322917 8.297841620756553E-5 32-33 0.16130312623949009 1.3829736034594255E-5 0.0 0.5231443398486142 8.297841620756553E-5 34-35 0.16236801591415384 2.0744604051891382E-5 0.0 0.8520362073553174 8.297841620756553E-5 36-37 0.16449779526348135 2.765947206918851E-5 0.0 1.2964962640351076 8.297841620756553E-5 38-39 0.16671055302901644 2.765947206918851E-5 0.0 1.812310843785387 8.297841620756553E-5 40-41 0.16888182158644774 2.765947206918851E-5 0.0 2.4162761610201695 8.297841620756553E-5 42-43 0.1733073371175179 3.4574340086485636E-5 0.0 2.888845156190273 8.297841620756553E-5 44-45 0.17905359243989183 7.60635481902684E-5 0.0 3.3957603008022907 8.989328422486265E-5 46-47 0.18237964395621176 8.297841620756553E-5 0.0 3.9860549239668774 9.680815224215978E-5 48-49 0.18403229741234572 8.297841620756553E-5 0.0 4.497167393465395 9.680815224215978E-5 50-51 0.18647324582245164 8.297841620756553E-5 0.0 4.995390548979669 9.680815224215978E-5 52-53 0.18764877338539215 8.297841620756553E-5 0.0 5.549873070682674 9.680815224215978E-5 54-55 0.19078812346524504 8.297841620756553E-5 0.0 6.080146639457122 9.680815224215978E-5 56-57 0.19653437878761895 8.297841620756553E-5 0.0 6.636724366169368 9.680815224215978E-5 58-59 0.19873330681711943 8.297841620756553E-5 0.0 7.19607495495655 9.680815224215978E-5 60-61 0.1997359626796275 8.297841620756553E-5 0.0 7.685032187327646 9.680815224215978E-5 62-63 0.20046893868946103 8.297841620756553E-5 0.0 8.181671838065961 9.680815224215978E-5 64-65 0.20111893628308694 1.1063788827675404E-4 0.0 8.725201208829567 9.680815224215978E-5 66-67 0.20192106097309342 1.1063788827675404E-4 0.0 9.284026267647434 9.680815224215978E-5 68-69 0.20272318566309988 1.1063788827675404E-4 0.0 9.894705921727013 1.0372302025945691E-4 70-71 0.2038710537539712 1.1063788827675404E-4 0.0 10.59910278204502 1.244676243113483E-4 72-73 0.20624285348390414 1.1063788827675404E-4 0.0 11.188374004743046 1.3829736034594255E-4 74-75 0.20870454649806192 1.1063788827675404E-4 0.0 11.75941072047946 1.3829736034594255E-4 76-77 0.20970720236057 1.244676243113483E-4 0.0 12.394416880243892 1.3829736034594255E-4 78-79 0.2105715608627321 1.244676243113483E-4 0.0 13.02016403173316 1.3829736034594255E-4 80-81 0.21197527907024344 1.244676243113483E-4 0.0 13.691507822928486 1.3829736034594255E-4 82-83 0.21264602126792126 1.3138249232864542E-4 0.0 14.424193408305253 1.3829736034594255E-4 84-85 0.2149140979775947 1.4521222836323967E-4 0.0 15.114601490624269 1.3829736034594255E-4 86-87 0.2196162082293568 1.5904196439783393E-4 0.0 15.802368093360677 1.3829736034594255E-4 88 0.22070875737608972 2.0744604051891382E-4 0.0 16.34421715119608 1.3829736034594255E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTACA 8725 0.0 24.639585 2 TCTACAC 10315 0.0 24.03055 3 TACACTC 10350 0.0 23.543228 5 CTACACT 12845 0.0 19.739414 4 GAGTACT 31260 0.0 19.049744 12-13 AGTACTT 32195 0.0 18.824976 12-13 CACTCTT 12540 0.0 18.781511 7 AGTACTC 3000 0.0 17.561974 5 ACACTCT 13990 0.0 17.372536 6 ACTCTTT 15640 0.0 17.222979 8 GTATAAG 1820 0.0 17.074347 1 GTACTTT 35970 0.0 16.661482 14-15 TACTTTT 37455 0.0 16.333332 14-15 GTACCGT 360 4.028516E-8 15.670005 6 TAGTACT 1630 0.0 15.584245 4 ACTTTTT 39865 0.0 15.225218 16-17 CGTCGTG 2610 0.0 14.769429 7 TATCACG 415 1.4280886E-8 14.73594 2 CATGGGT 8880 0.0 14.567981 4 TATTCGC 355 5.865131E-7 14.566483 9 >>END_MODULE