##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765251_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7750898 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20583150494304 33.0 31.0 34.0 31.0 34.0 2 32.19333205004117 34.0 31.0 34.0 30.0 34.0 3 32.208542287616226 34.0 31.0 34.0 30.0 34.0 4 35.68227449258138 37.0 35.0 37.0 35.0 37.0 5 35.71213064602321 37.0 35.0 37.0 35.0 37.0 6 35.82657093410338 37.0 37.0 37.0 35.0 37.0 7 35.784857186870475 37.0 36.0 37.0 35.0 37.0 8 35.825804442272364 37.0 37.0 37.0 35.0 37.0 9 37.50708769487097 39.0 38.0 39.0 35.0 39.0 10-11 37.44013745246035 39.0 38.0 39.0 35.0 39.0 12-13 37.41873386799826 39.0 38.0 39.0 35.0 39.0 14-15 38.7607344981188 41.0 38.5 41.0 35.0 41.0 16-17 38.71122687977574 40.0 38.0 41.0 35.0 41.0 18-19 38.7103184043965 40.0 38.5 41.0 35.0 41.0 20-21 38.7021245151207 40.0 39.0 41.0 35.0 41.0 22-23 38.610244903235724 40.0 38.5 41.0 34.5 41.0 24-25 38.53202848237714 40.0 38.0 41.0 34.0 41.0 26-27 38.39210108299709 40.0 38.0 41.0 34.0 41.0 28-29 38.24531596209884 40.0 38.0 41.0 34.0 41.0 30-31 37.99879246249918 40.0 38.0 41.0 33.5 41.0 32-33 37.68352783122678 40.0 37.0 41.0 33.0 41.0 34-35 37.54365855414431 40.0 37.0 41.0 33.0 41.0 36-37 37.394071241809655 40.0 37.0 41.0 32.5 41.0 38-39 37.19299344411448 40.0 37.0 41.0 32.0 41.0 40-41 36.91204070805731 40.0 36.0 41.0 31.0 41.0 42-43 36.622113398989384 40.0 36.0 41.0 30.5 41.0 44-45 36.214787112409425 39.5 35.0 41.0 30.0 41.0 46-47 35.731102383233534 39.0 35.0 41.0 28.5 41.0 48-49 35.296522802906196 39.0 35.0 41.0 26.5 41.0 50-51 34.034810611622035 38.0 34.0 40.0 24.5 40.5 52-53 33.96729985351375 38.0 33.5 39.5 23.0 40.0 54-55 34.74877330342884 39.0 35.0 40.5 24.5 41.0 56-57 34.74649898631101 38.5 35.0 41.0 24.5 41.0 58-59 34.54070967002791 38.0 35.0 41.0 24.0 41.0 60-61 34.17991321779748 37.5 35.0 40.0 23.0 41.0 62-63 33.70559101667962 37.0 34.0 40.0 22.0 41.0 64-65 33.44355434686407 36.0 34.0 39.5 21.0 41.0 66-67 33.150721568004116 36.0 34.0 39.0 20.0 41.0 68-69 32.930301688913985 35.5 34.0 39.0 20.0 41.0 70-71 32.55249694938574 35.0 34.0 38.5 18.5 40.5 72-73 32.175060038204606 35.0 33.5 37.0 17.5 39.0 74-75 31.795221082770023 35.0 33.0 37.0 12.0 39.0 76-77 31.40639858762172 35.0 33.0 36.0 7.5 38.5 78-79 31.09495590059371 35.0 33.0 36.0 4.5 37.0 80-81 30.796826767685502 35.0 33.0 35.0 2.0 37.0 82-83 30.529802159698143 35.0 32.5 35.0 2.0 36.5 84-85 30.261475573539997 35.0 32.0 35.0 2.0 36.0 86-87 29.989433611434443 35.0 32.0 35.0 2.0 36.0 88-89 29.75648105548544 35.0 32.0 35.0 2.0 35.5 90-91 29.50234521212897 35.0 31.0 35.0 2.0 35.0 92-93 29.179553517540807 35.0 31.0 35.0 2.0 35.0 94-95 28.882643985251775 35.0 31.0 35.0 2.0 35.0 96-97 28.609160706282033 35.0 31.0 35.0 2.0 35.0 98-99 28.278425674031574 35.0 30.0 35.0 2.0 35.0 100 27.721807331227943 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41772192285829846 1101 2 0.5652742122574068 1101 3 0.5909949102540466 1101 4 0.4501569315619136 1101 5 0.5056773319580259 1101 6 0.5047297303939757 1101 7 0.5531278018553962 1101 8 0.517082809908807 1101 9 0.6084958032835601 1101 10-11 0.6033893268656598 1101 12-13 0.6088284806873574 1101 14-15 0.7763513041340886 1101 16-17 0.7578425726262807 1101 18-19 0.8321024021741366 1101 20-21 0.8032960876099224 1101 22-23 0.7662552967311527 1101 24-25 0.8152344312643081 1101 26-27 0.8398537667961605 1101 28-29 0.8839522145550731 1101 30-31 0.8906812045449897 1101 32-33 0.9297349675575646 1101 34-35 1.0517678921451434 1101 36-37 1.0622846309041876 1101 38-39 1.046948751897233 1101 40-41 1.1321262640476348 1101 42-43 1.1370533143464598 1101 44-45 1.2657021879849069 1101 46-47 1.2330200668843574 1101 48-49 1.3206604214797224 1101 50-51 1.2596613466447053 1101 52-53 1.2913008291071435 1101 54-55 1.36813633646058 1101 56-57 1.4186884188759308 1101 58-59 1.452916614026016 1101 60-61 1.4415190993256388 1101 62-63 1.4757241903604665 1101 64-65 1.4264185204683528 1101 66-67 1.4057558745121312 1101 68-69 1.3536033892355022 1101 70-71 1.293865261387701 1101 72-73 1.2723428399019596 1101 74-75 1.2712840213148375 1101 76-77 1.1773681394909765 1101 78-79 1.21928827321916 1101 80-81 1.3149331170548635 1101 82-83 1.2746634180238914 1101 84-85 1.265052757652061 1101 86-87 1.276572403206 1101 88-89 1.296147826281981 1101 90-91 1.2901400863720625 1101 92-93 1.373417935115807 1101 94-95 1.2910393021448279 1101 96-97 1.371928734935068 1101 98-99 1.323936300427956 1101 100 1.308800224482269 1102 1 0.4576718872763905 1102 2 0.6058678660540977 1102 3 0.61964318031049 1102 4 0.5140918943784243 1102 5 0.517226018819855 1102 6 0.4623217739317198 1102 7 0.4815657559981261 1102 8 0.4643126576087653 1102 9 0.60995182136012 1102 10-11 0.5716921121560503 1102 12-13 0.6399916462308113 1102 14-15 0.8037121214687133 1102 16-17 0.8047229720430806 1102 18-19 0.8170644100037308 1102 20-21 0.7858972189332079 1102 22-23 0.8015072261227658 1102 24-25 0.7543076585987905 1102 26-27 0.7853710624841952 1102 28-29 0.7910183401032 1102 30-31 0.8651822244677021 1102 32-33 0.879936984641617 1102 34-35 0.9083957231160795 1102 36-37 0.9090346602927966 1102 38-39 0.8977653310949876 1102 40-41 0.9900000289372031 1102 42-43 1.006757078178822 1102 44-45 0.976220320952379 1102 46-47 1.0252850250933605 1102 48-49 1.10825656068468 1102 50-51 1.120416107204484 1102 52-53 1.2066128256546662 1102 54-55 1.2665804069837705 1102 56-57 1.2794379279481305 1102 58-59 1.295645458568579 1102 60-61 1.2593836541415087 1102 62-63 1.28313187463295 1102 64-65 1.2768789353960344 1102 66-67 1.2531424433666771 1102 68-69 1.1697497460739328 1102 70-71 1.1262677089655213 1102 72-73 1.1823970372261385 1102 74-75 1.1467567538161951 1102 76-77 1.1356274917739775 1102 78-79 1.147819176226502 1102 80-81 1.125741965471196 1102 82-83 1.1028968008379074 1102 84-85 1.1414861918665515 1102 86-87 1.1529241558320429 1102 88-89 1.088025555083128 1102 90-91 1.1163746494640776 1102 92-93 1.062842198507152 1102 94-95 1.0356941014028926 1102 96-97 1.155288008480273 1102 98-99 1.1796916694699568 1102 100 1.2094902551568723 1103 1 0.38576994528268926 1103 2 0.46129388812243377 1103 3 0.5547774043883109 1103 4 0.429118597614746 1103 5 0.4631557533975368 1103 6 0.4241794299202226 1103 7 0.49667726202442 1103 8 0.4676973447025361 1103 9 0.5507658384894327 1103 10-11 0.5509323672246822 1103 12-13 0.5696861596009697 1103 14-15 0.6990248843469615 1103 16-17 0.770319730177313 1103 18-19 0.7983908459251907 1103 20-21 0.7512457931410665 1103 22-23 0.7890130098822254 1103 24-25 0.7404808370933722 1103 26-27 0.7316955215728171 1103 28-29 0.7050542338027768 1103 30-31 0.7945079057045632 1103 32-33 0.8666807176706186 1103 34-35 0.8340096538036121 1103 36-37 0.8090131875969249 1103 38-39 0.8745123533198012 1103 40-41 0.8572069894433625 1103 42-43 0.86658605353783 1103 44-45 0.875800528230549 1103 46-47 0.806663493253609 1103 48-49 0.7920721529764378 1103 50-51 0.7317635105819065 1103 52-53 0.7416843897116436 1103 54-55 0.8678791980642231 1103 56-57 0.8240610243505699 1103 58-59 0.8406217909742182 1103 60-61 0.8590063165139199 1103 62-63 0.948902160727144 1103 64-65 0.9140889833756134 1103 66-67 0.7362565273305464 1103 68-69 0.7462788123807869 1103 70-71 0.7465889458189565 1103 72-73 0.7108712981715328 1103 74-75 0.7426553031379548 1103 76-77 0.7820001144206152 1103 78-79 0.7616996666304665 1103 80-81 0.726550937672684 1103 82-83 0.7535656903733674 1103 84-85 0.7343527064102453 1103 86-87 0.7374982687122547 1103 88-89 0.8083753039037163 1103 90-91 0.8344122719993443 1103 92-93 0.7786897321402755 1103 94-95 0.7237064418641737 1103 96-97 0.7295547720670825 1103 98-99 0.742980228368392 1103 100 0.7704126383071888 1104 1 0.252133918635991 1104 2 0.30457451657020584 1104 3 0.34121982398236383 1104 4 0.2968264591525127 1104 5 0.38417006545984833 1104 6 0.3296021164933336 1104 7 0.38321431505862336 1104 8 0.3625514953115996 1104 9 0.4213764606674104 1104 10-11 0.48865986176431164 1104 12-13 0.46587729291555746 1104 14-15 0.552101328818658 1104 16-17 0.5346603603631266 1104 18-19 0.5550757279209577 1104 20-21 0.48238377697076373 1104 22-23 0.4938347810473971 1104 24-25 0.5497568061401736 1104 26-27 0.5452552524521153 1104 28-29 0.5932553496020532 1104 30-31 0.5623018114018308 1104 32-33 0.5719307231256252 1104 34-35 0.6581852499797733 1104 36-37 0.6952867603479405 1104 38-39 0.6036394869078379 1104 40-41 0.6075932863497044 1104 42-43 0.6705422181881602 1104 44-45 0.7722317451827792 1104 46-47 0.794921688039075 1104 48-49 0.8351153906080313 1104 50-51 0.69957710477712 1104 52-53 0.8355470333661046 1104 54-55 0.91314168004849 1104 56-57 0.8902577652924393 1104 58-59 0.8765358941316066 1104 60-61 0.9237419022568147 1104 62-63 0.9497397732501867 1104 64-65 0.9923489653873148 1104 66-67 0.9547566333406863 1104 68-69 0.9100815971996354 1104 70-71 0.8419129338635898 1104 72-73 0.8753193195910001 1104 74-75 0.7871632590695192 1104 76-77 0.7712313510991926 1104 78-79 0.7648622498572557 1104 80-81 0.8327844788262482 1104 82-83 0.7992718257305924 1104 84-85 0.8153315867439055 1104 86-87 0.6923074271304976 1104 88-89 0.6582606603405736 1104 90-91 0.6078630262226952 1104 92-93 0.5989225836288377 1104 94-95 0.5820205502180116 1104 96-97 0.5577577759945669 1104 98-99 0.5892209108592645 1104 100 0.6574550841105307 1105 1 0.1946491849209906 1105 2 0.26017395022760326 1105 3 0.33992596023705346 1105 4 0.25775654948080273 1105 5 0.3575712235939008 1105 6 0.3084705072739595 1105 7 0.30288591765283 1105 8 0.3426668266392454 1105 9 0.41509396578589275 1105 10-11 0.41765703443382307 1105 12-13 0.42623502978779726 1105 14-15 0.4798367321526342 1105 16-17 0.49027513284379154 1105 18-19 0.5452068385907936 1105 20-21 0.5016995490893521 1105 22-23 0.46868016873123963 1105 24-25 0.49809272138282523 1105 26-27 0.4633241746965169 1105 28-29 0.4613633820677663 1105 30-31 0.5068957715928235 1105 32-33 0.5453544172401763 1105 34-35 0.5131962697531094 1105 36-37 0.5133392254706308 1105 38-39 0.46235711821270087 1105 40-41 0.4729210626464848 1105 42-43 0.5044992295869406 1105 44-45 0.4200695340286984 1105 46-47 0.4294550500552461 1105 48-49 0.46364876033140234 1105 50-51 0.4567664792493247 1105 52-53 0.49558060347646204 1105 54-55 0.512970841070775 1105 56-57 0.4578361436569267 1105 58-59 0.46699660437806045 1105 60-61 0.4816619411977854 1105 62-63 0.5478163037020636 1105 64-65 0.4762305634237052 1105 66-67 0.41170790469490015 1105 68-69 0.3922202364359464 1105 70-71 0.38969491735024775 1105 72-73 0.30007661104798444 1105 74-75 0.3168884440270112 1105 76-77 0.3502721751884259 1105 78-79 0.4003475067545885 1105 80-81 0.3798946142975268 1105 82-83 0.373321166266976 1105 84-85 0.4050488929866134 1105 86-87 0.3258587077635653 1105 88-89 0.3634193861815511 1105 90-91 0.3848043469875577 1105 92-93 0.3928930395440027 1105 94-95 0.3928401745724912 1105 96-97 0.35595375658816053 1105 98-99 0.356981416970914 1105 100 0.3566422473742463 1106 1 0.25588568884053586 1106 2 0.33508933617106607 1106 3 0.34994281205746347 1106 4 0.30675145318590324 1106 5 0.37427513445431515 1106 6 0.3115600313452731 1106 7 0.30608182159134856 1106 8 0.3339408023967039 1106 9 0.30773037764443956 1106 10-11 0.42273364710575834 1106 12-13 0.4039780153187067 1106 14-15 0.46064348980502956 1106 16-17 0.435826850000133 1106 18-19 0.43620110664335954 1106 20-21 0.43174174182226466 1106 22-23 0.4796089341880929 1106 24-25 0.5484311811585201 1106 26-27 0.5301030448507547 1106 28-29 0.5650972763617474 1106 30-31 0.5463521424299884 1106 32-33 0.5150197739353786 1106 34-35 0.5386401900638305 1106 36-37 0.5988211457915824 1106 38-39 0.5860578959606286 1106 40-41 0.6123917062045692 1106 42-43 0.6006504436165585 1106 44-45 0.6105197562018247 1106 46-47 0.4789184759682925 1106 48-49 0.5631809160865728 1106 50-51 0.5290915136515153 1106 52-53 0.5614901900311295 1106 54-55 0.585009728893219 1106 56-57 0.6454397371312197 1106 58-59 0.6527667690599159 1106 60-61 0.7362914354188419 1106 62-63 0.7737298891742554 1106 64-65 0.7271373124720029 1106 66-67 0.7352476821753342 1106 68-69 0.6080953178625137 1106 70-71 0.6275460667497512 1106 72-73 0.6300067233442306 1106 74-75 0.6780940420572144 1106 76-77 0.6941512011534918 1106 78-79 0.6321804379007965 1106 80-81 0.5820409248435325 1106 82-83 0.5687514534848077 1106 84-85 0.6182056085272016 1106 86-87 0.6435652870300181 1106 88-89 0.6384065510263994 1106 90-91 0.6087398951991858 1106 92-93 0.6232024526820901 1106 94-95 0.588851646560336 1106 96-97 0.5850225504640854 1106 98-99 0.5862969042325936 1106 100 0.6379145207698649 1107 1 0.22226055075100248 1107 2 0.3082733989113393 1107 3 0.30078832068043226 1107 4 0.2751463807740251 1107 5 0.28838634702828614 1107 6 0.20187481708444466 1107 7 0.31097868274611074 1107 8 0.255582594073104 1107 9 0.32370949207101773 1107 10-11 0.3114341678367367 1107 12-13 0.39888708147069707 1107 14-15 0.4255275890509367 1107 16-17 0.38083876029126174 1107 18-19 0.4022346697594372 1107 20-21 0.3717236146032761 1107 22-23 0.3961687042949791 1107 24-25 0.4570715158255041 1107 26-27 0.4120204922008597 1107 28-29 0.40893001010947927 1107 30-31 0.4746395484985442 1107 32-33 0.4593850404450137 1107 34-35 0.4318751392905895 1107 36-37 0.48810978380279124 1107 38-39 0.5326204919284336 1107 40-41 0.4767732341946598 1107 42-43 0.4978332275252768 1107 44-45 0.48545436986987056 1107 46-47 0.40790899563995 1107 48-49 0.41026662302289907 1107 50-51 0.295442548547328 1107 52-53 0.35370165251791974 1107 54-55 0.4298931043233125 1107 56-57 0.4250889749020459 1107 58-59 0.3347779756111251 1107 60-61 0.40553215093211037 1107 62-63 0.4489528425587963 1107 64-65 0.4719138201189068 1107 66-67 0.4471520419333288 1107 68-69 0.40098859927746844 1107 70-71 0.39638822313722955 1107 72-73 0.40215474259583317 1107 74-75 0.3969567923250814 1107 76-77 0.3477227006991086 1107 78-79 0.35125144602693226 1107 80-81 0.3658429458469925 1107 82-83 0.33122351438040454 1107 84-85 0.4125075546946029 1107 86-87 0.418213289269584 1107 88-89 0.4147478523260446 1107 90-91 0.41612281485891245 1107 92-93 0.48936717271416086 1107 94-95 0.42154255103591254 1107 96-97 0.46955221795806423 1107 98-99 0.4097502024205717 1107 100 0.41577947468111276 1108 1 0.13344595972611017 1108 2 0.15308410084347912 1108 3 0.18079653962589504 1108 4 0.26425320908969496 1108 5 0.2991951956057619 1108 6 0.2617407155778366 1108 7 0.35362250004292406 1108 8 0.2776403204737008 1108 9 0.2984441263769 1108 10-11 0.3447503442675597 1108 12-13 0.34035800380912207 1108 14-15 0.38702020741785503 1108 16-17 0.38621388339641527 1108 18-19 0.39176559799901156 1108 20-21 0.35928624862330594 1108 22-23 0.38346886985762296 1108 24-25 0.41886847192209586 1108 26-27 0.43806646773078484 1108 28-29 0.3891285479146589 1108 30-31 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-1.053017948094741 2316 72-73 -1.0077559355441643 2316 74-75 -0.9321353753045543 2316 76-77 -0.9212484155354481 2316 78-79 -0.883845786647921 2316 80-81 -0.8390057664555215 2316 82-83 -0.8610504798776191 2316 84-85 -0.7980794052031399 2316 86-87 -0.9047538370472843 2316 88-89 -0.8806636892342716 2316 90-91 -0.8277434698056538 2316 92-93 -0.7711614515606264 2316 94-95 -0.6846410382706836 2316 96-97 -0.6834712729146339 2316 98-99 -0.7792703364970528 2316 100 -0.9287456308738449 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21901.0 3 4315.0 4 4135.0 5 4738.0 6 5608.0 7 6726.0 8 8234.0 9 10022.0 10 12996.0 11 18550.0 12 27528.0 13 38988.0 14 41340.0 15 50349.0 16 66197.0 17 91349.0 18 89852.0 19 52457.0 20 44420.0 21 40617.0 22 41712.0 23 47316.0 24 52871.0 25 56618.0 26 62978.0 27 73540.0 28 86966.0 29 103821.0 30 126830.0 31 156506.0 32 197426.0 33 259983.0 34 372604.0 35 588888.0 36 1060440.0 37 1896557.0 38 1610264.0 39 314876.0 40 380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.26227223903361 17.512736841180722 13.185233272385247 24.03975764740042 2 14.543612890660611 21.97271695079603 36.850802558407445 26.632867600135917 3 19.357237120718615 27.70917864510957 29.059471528237353 23.87411270593446 4 13.372356322828969 18.613510135560833 33.968222065062 34.0459114765482 5 13.366046104923726 36.5506859238759 33.47587260550393 16.60739536569644 6 30.978834379289243 33.354857080253346 18.485591233814556 17.180717306642855 7 24.93597874826517 32.379706655457966 22.395983474835408 20.28833112144146 8 27.83839960108874 30.594672312742293 21.511096011274926 20.055832074894038 9 28.20236517357414 14.387423871104865 19.514453177723638 37.89575777759735 10-11 24.664608240148755 25.931047990771667 25.652989108740964 23.751354660338617 12-13 27.594624697561983 23.76482062671261 26.091375848610827 22.549178827114584 14-15 26.309901889072414 22.81525812214824 25.10432789731269 25.770512091466657 16-17 22.8349841104107 26.167059764195745 27.833178335794756 23.164777789598805 18-19 24.379251115034386 25.00278136746109 29.485318450099296 21.13264906740523 20-21 24.088789415326058 24.606003414152518 28.683965797484756 22.62124137303667 22-23 22.76792201605247 25.638309550902616 26.289330209740225 25.304438223304686 24-25 22.79226586743075 26.667293493025422 25.743170500646862 24.797270138896966 26-27 24.05131040992602 24.5746899281509 26.358517393127112 25.015482268795964 28-29 23.196146402432014 23.97948273746252 29.094167925343882 23.730202934761586 30-31 24.902390652414393 24.391142845081117 27.81659366343641 22.889872839068072 32-33 21.553018257542693 25.621955468111963 28.237320321264274 24.58770595308107 34-35 21.260724145459527 26.305067622995825 28.868740722750243 23.565467508794413 36-37 23.652440302753032 25.897654815233885 27.09871834945192 23.351186532561165 38-39 24.477896309476847 24.429028078088454 26.6710914292392 24.421984183195498 40-41 22.90301547660883 25.86898511814623 26.847670974164505 24.380328431080432 42-43 25.322301217133347 24.95583004651725 26.42970689536743 23.29216184098197 44-45 25.11305435765353 24.57646183542912 26.741292809527184 23.569190997390162 46-47 23.329560847945498 25.111322126818692 28.806075236825794 22.753041788410016 48-49 24.30779736214845 25.85786034608451 27.559402864185117 22.274939427581923 50-51 22.661848745360818 26.55034163383238 26.391515667722405 24.396293953084395 52-53 22.160054846956402 26.945982645143786 24.55012230907443 26.343840198825387 54-55 23.200438868466552 26.56027544560234 24.296628919322547 25.942656766608557 56-57 24.20261583773618 24.58173987766831 24.96511176983665 26.250532514758866 58-59 23.724193585468406 24.321472863951783 27.229688939726117 24.724644610853698 60-61 24.025293833562753 25.217175995392672 26.146499693963726 24.611030477080853 62-63 21.509451286602328 25.678789012094793 26.98051371111963 25.831245990183245 64-65 21.303088370504756 25.88852305897295 27.17093033663054 25.637458233891756 66-67 23.800787581517234 25.330477705132903 25.810513308575285 25.058221404774578 68-69 24.372710516871944 24.25343495914749 25.61749459214836 25.756359931832208 70-71 22.788260758330896 25.303801472084718 26.029386793272387 25.878550976312003 72-73 24.68820393436705 24.62041368397856 25.919999235074275 24.77138314658012 74-75 25.097959881449555 24.003905145426593 26.0970036314233 24.801131341700547 76-77 23.402488087163583 25.137917185185128 27.69379793104509 23.765796796606196 78-79 23.80764586433181 25.181196944548716 27.25106296651163 23.760094224607844 80-81 22.633746078236847 25.381328936873615 26.874644917844993 25.11028006704455 82-83 22.658177048382335 25.913107756956578 25.051517950751144 26.37719724390994 84-85 23.222277271479676 26.01978749435939 24.663889392860963 26.094045841299966 86-87 23.81737532603518 24.317720262270438 25.451770832067876 26.413133579626507 88-89 23.968536873516562 23.701885972011024 26.662370014146646 25.667207140325765 90-91 24.692737256870625 24.650213790282663 25.704492022617053 24.952556930229655 92-93 23.115275950069716 25.037251020314727 26.21542612939443 25.632046900221123 94-95 22.604194621510672 25.331234559702224 26.69422051441185 25.370350304375254 96-97 24.75492491764914 24.767378929559552 25.2751453070545 25.202550845736805 98-99 25.21597873535783 24.066744960280325 24.93814562767553 25.779130676686314 100 24.591543994847438 24.757256342301744 25.630137552257022 25.02106211059379 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1720.0 1 2159.5 2 1814.0 3 979.0 4 804.0 5 793.5 6 1046.5 7 1711.5 8 2497.0 9 2663.0 10 2333.5 11 2200.5 12 2426.5 13 2756.5 14 3162.5 15 3791.5 16 4467.0 17 5147.5 18 6036.0 19 7073.5 20 8288.5 21 9858.0 22 11864.0 23 14302.5 24 17589.0 25 21634.0 26 26764.0 27 32809.5 28 38883.5 29 46754.0 30 55669.5 31 63826.0 32 72795.5 33 81085.0 34 88823.5 35 94960.5 36 99555.5 37 107833.0 38 114239.5 39 118093.0 40 121063.0 41 123725.5 42 130039.5 43 146260.5 44 169056.0 45 190328.5 46 221170.0 47 265384.5 48 380806.0 49 539053.5 50 772436.5 51 729735.5 52 430794.0 53 321778.0 54 281888.5 55 246993.0 56 223201.0 57 199018.5 58 183950.0 59 169809.5 60 144946.5 61 119211.5 62 97034.0 63 76505.5 64 57274.5 65 46148.0 66 36727.0 67 26776.5 68 20514.0 69 16788.5 70 14282.0 71 12249.0 72 10308.0 73 10332.0 74 8671.0 75 6777.5 76 5285.0 77 3621.0 78 2739.5 79 2010.5 80 1452.5 81 1107.5 82 876.0 83 673.5 84 462.5 85 312.0 86 224.5 87 146.0 88 102.5 89 88.5 90 73.0 91 63.5 92 58.0 93 43.5 94 36.0 95 35.0 96 33.5 97 24.0 98 17.5 99 16.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1134062143509049 2 0.36891209250850676 3 0.3791560668196124 4 0.3757629116006945 5 0.3023778664098018 6 0.1368873645350513 7 0.15057610098855642 8 0.1267982109943906 9 0.2062986766178577 10-11 0.20675668806375727 12-13 0.22038736672834552 14-15 0.1496213729041461 16-17 0.13485534192296172 18-19 0.15351769562700993 20-21 0.1539821579383447 22-23 0.1624005373312873 24-25 0.1742765805974998 26-27 0.16790312554751718 28-29 0.17557320454997602 30-31 0.21777476622708752 32-33 0.2147170560108003 34-35 0.13144283410773824 36-37 0.09153132966012455 38-39 0.08519012893731796 40-41 0.08429345864182447 42-43 0.0750106632805644 44-45 0.09675007979720543 46-47 0.14300923583306088 48-49 0.22165173635364574 50-51 0.17821160851297488 52-53 0.11130323221902805 54-55 0.05952213537063705 56-57 0.07492680202990673 58-59 0.11018078163330236 60-61 0.08667382798741513 62-63 0.09549861190277566 64-65 0.10420728024030249 66-67 0.10097539665726474 68-69 0.09115072859944744 70-71 0.1251403385775429 72-73 0.14946010126826595 74-75 0.19288732737806638 76-77 0.19242931593216683 78-79 0.15408537178530798 80-81 0.09149262446751331 82-83 0.13094611746922744 84-85 0.15964601779045473 86-87 0.17076730980074825 88-89 0.15889126653453575 90-91 0.1805403709350839 92-93 0.10091088800291269 94-95 0.3404379724775117 96-97 0.34163138258302456 98-99 0.3810655229884331 100 0.383013684349865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7750898.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.63906450105635 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33831655852293 26.35227683616324 2 12.106076772591623 8.144741948158995 3 3.6040264632296495 3.637082359802885 4 1.5974105003795744 2.149415794277328 5 0.8648850925828032 1.4546962707697506 6 0.6010875997882945 1.2132014724038152 7 0.41577667711766436 0.9790436921717194 8 0.31609412777131335 0.8506488602003485 9 0.2384046557326422 0.7217738632388236 >10 1.5477598811696855 10.108016172233816 >50 0.1808385207488597 4.26517842199877 >100 0.1547935938626706 10.790556878230321 >500 0.020934040575521926 4.835017428179392 >1k 0.011432592938713754 7.027243699536324 >5k 6.179779966830961E-4 1.3982404309656533 >10k+ 0.0015449449917077404 16.072865871668927 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 154806 1.9972653491247079 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 147542 1.9035471760820488 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 64628 0.8338130626928647 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 62095 0.8011329783981159 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 52457 0.6767860962691032 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 50896 0.6566464943803931 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 49195 0.6347006501698255 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 43039 0.5552775949315808 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 42325 0.546065759090108 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 40239 0.5191527484944325 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 38210 0.49297513655837044 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 36012 0.46461713210520894 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 30239 0.3901354397903314 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29145 0.3760209462181027 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 23892 0.30824815395583843 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 22215 0.2866119512861606 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 21332 0.2752197229275885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19993 0.2579443052921094 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 19737 0.2546414621892844 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 19081 0.2461779267382954 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 17968 0.23181830027952888 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 17458 0.22523841753561974 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 16864 0.21757478939859612 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 15072 0.19445488767882121 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 14910 0.19236480727781477 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 14690 0.18952642648632456 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 14537 0.18755246166315181 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 14508 0.1871783114679099 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 14311 0.18463667048643911 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13897 0.17929535390608933 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 12856 0.1658646520699924 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 12479 0.1610006995318478 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 12460 0.16075556664531002 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12148 0.15673022661374206 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 12114 0.15629156776414813 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 11557 0.14910530366932967 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 10736 0.13851298262472297 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 10505 0.13553268279365824 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 10504 0.13551978106278784 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 10424 0.13448764259315502 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 8962 0.11562531206061542 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8867 0.11439964762792647 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 8479 0.10939377605020734 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 8375 0.1080519960396847 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5482077044492136E-4 0.0 0.0 0.0 0.0 2 3.225432717602528E-4 0.0 0.0 0.0 0.0 3 3.225432717602528E-4 0.0 0.0 0.0 0.0 4 3.4834673350107306E-4 0.0 0.0 0.0 0.0 5 0.0015224042427083933 0.0 0.0 1.2901730870410112E-5 0.0 6 0.002993201561935146 0.0 0.0 1.2901730870410112E-5 1.2901730870410112E-5 7 0.0033802534880474494 0.0 0.0 1.2901730870410112E-5 1.2901730870410112E-5 8 0.0038318140685118032 0.0 0.0 2.5803461740820224E-5 1.2901730870410112E-5 9 0.004154357340272056 0.0 0.0 3.870519261123034E-5 1.2901730870410112E-5 10-11 0.00681211389957654 0.0 0.0 1.032138469632809E-4 1.2901730870410112E-5 12-13 0.014578955883563427 0.0 0.0 1.8062423218574158E-4 1.2901730870410112E-5 14-15 0.02420364711288937 0.0 0.0 2.709363482786124E-4 1.2901730870410112E-5 16-17 0.027880640410956253 0.0 0.0 4.451097150291489E-4 1.2901730870410112E-5 18-19 0.02990621215761064 0.0 0.0 6.192830817796854E-4 1.2901730870410112E-5 20-21 0.03739566692788371 0.0 0.0 0.001032138469632809 1.2901730870410112E-5 22-23 0.039963111371095324 0.0 0.0 0.0028319299260550195 1.2901730870410112E-5 24-25 0.05712886429417598 0.0 0.0 0.006721801783483668 1.2901730870410112E-5 26-27 0.11139354433512091 0.0 0.0 0.013746794242421975 1.2901730870410112E-5 28-29 0.12263095192324812 0.0 0.0 0.04149841734467413 1.2901730870410112E-5 30-31 0.1246436219390321 0.0 0.0 0.12061183104202894 1.2901730870410112E-5 32-33 0.12615312445087007 0.0 0.0 0.2466165855878893 2.5803461740820224E-5 34-35 0.1274884535959575 0.0 0.0 0.4120941857317694 2.5803461740820224E-5 36-37 0.12958498486239917 0.0 0.0 0.6560336621640486 2.5803461740820224E-5 38-39 0.13170731959058163 6.450865435205056E-6 0.0 1.0026064076704402 2.5803461740820224E-5 40-41 0.13381675258789366 1.2901730870410112E-5 0.0 1.4925238340125233 2.5803461740820224E-5 42-43 0.13838396531601888 1.2901730870410112E-5 0.0 1.8477665426638308 2.5803461740820224E-5 44-45 0.1436930275691926 1.2901730870410112E-5 0.0 2.208769874148776 3.225432717602528E-5 46-47 0.14601533912586645 1.2901730870410112E-5 0.0 2.626502116270915 3.870519261123034E-5 48-49 0.1478473849094647 1.2901730870410112E-5 0.0 3.00355520095865 3.870519261123034E-5 50-51 0.15028581204397218 1.2901730870410112E-5 0.0 3.4200488769172295 3.870519261123034E-5 52-53 0.15120828580120654 1.2901730870410112E-5 0.0 3.915688220900339 3.870519261123034E-5 54-55 0.15395635447660388 2.5803461740820224E-5 0.0 4.349302493723953 3.870519261123034E-5 56-57 0.16016208702527113 2.5803461740820224E-5 0.0 4.80660434442564 3.870519261123034E-5 58-59 0.16251020204368577 2.5803461740820224E-5 0.0 5.2832007336440245 3.870519261123034E-5 60-61 0.16385843291964364 2.5803461740820224E-5 0.0 5.709099771407132 3.870519261123034E-5 62-63 0.16476800494600755 2.5803461740820224E-5 0.0 6.154738457401969 3.870519261123034E-5 64-65 0.1654582475475745 2.5803461740820224E-5 0.0 6.648971512720204 3.870519261123034E-5 66-67 0.1666258541913466 2.5803461740820224E-5 0.0 7.1421401752416305 3.870519261123034E-5 68-69 0.16781926429685956 2.5803461740820224E-5 0.0 7.727627173006276 3.870519261123034E-5 70-71 0.1691739460382526 2.5803461740820224E-5 0.0 8.448466229332395 5.160692348164045E-5 72-73 0.17285093933631948 3.225432717602528E-5 0.0 9.029095725424332 6.450865435205056E-5 74-75 0.17661824475047921 3.870519261123034E-5 0.0 9.575406617400976 6.450865435205056E-5 76-77 0.1779471230301315 6.450865435205056E-5 0.0 10.185516568531801 6.450865435205056E-5 78-79 0.17892765457628262 6.450865435205056E-5 0.0 10.7752095305602 6.450865435205056E-5 80-81 0.18044360795355585 6.450865435205056E-5 0.0 11.437629549505102 6.450865435205056E-5 82-83 0.18112094882425236 6.450865435205056E-5 0.0 12.215236221661026 6.450865435205056E-5 84-85 0.1837077458637696 6.450865435205056E-5 0.0 12.928708389660141 6.450865435205056E-5 86-87 0.18859750186365504 6.450865435205056E-5 0.0 13.625672793010565 7.095951978725562E-5 88 0.19002959399027056 6.450865435205056E-5 0.0 14.194768141704355 7.741038522246068E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8790 0.0 26.900393 1 ATCTACA 9700 0.0 24.950724 2 TCTACAC 11340 0.0 23.295527 3 TACACTC 11795 0.0 21.619158 5 GTATAAG 2145 0.0 21.080828 1 AGTACTT 33495 0.0 21.016026 12-13 CTACACT 13645 0.0 20.114052 4 GAGTACT 32585 0.0 20.059357 12-13 AGTACTC 2845 0.0 19.348219 5 CACTCTT 13800 0.0 18.538618 7 TACTTTT 39205 0.0 18.100838 14-15 GTGATCG 1500 0.0 17.868603 8 CATGGGG 16035 0.0 17.761963 4 GTACTTT 37410 0.0 17.61305 14-15 GTACTAG 2000 0.0 17.427916 1 ACTCTTT 16795 0.0 17.106777 8 ACACTCT 15230 0.0 17.104975 6 GTATTAG 2515 0.0 16.855757 1 ACTTTTT 40990 0.0 16.276436 16-17 TAGTACT 1915 0.0 16.224813 4 >>END_MODULE