##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765251_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7750898
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.505691985625404	34.0	31.0	34.0	31.0	34.0
2	32.63204895742403	34.0	31.0	34.0	31.0	34.0
3	32.66824050580978	34.0	31.0	34.0	31.0	34.0
4	36.17106043712612	37.0	35.0	37.0	35.0	37.0
5	36.112759321565065	37.0	35.0	37.0	35.0	37.0
6	36.17959880261616	37.0	37.0	37.0	35.0	37.0
7	36.130857095526224	37.0	36.0	37.0	35.0	37.0
8	36.14998791107818	37.0	37.0	37.0	35.0	37.0
9	37.875501393515954	39.0	38.0	39.0	35.0	39.0
10-11	37.830192191924084	39.0	38.0	39.0	35.0	39.0
12-13	37.798728740850414	39.0	38.0	39.0	35.0	39.0
14-15	39.14403789083536	40.5	38.5	41.0	36.0	41.0
16-17	39.112912852162424	40.0	38.5	41.0	36.0	41.0
18-19	39.154985073987554	40.0	39.0	41.0	36.0	41.0
20-21	39.14859768506823	40.0	39.0	41.0	36.0	41.0
22-23	39.091836197560596	40.0	39.0	41.0	35.5	41.0
24-25	39.03232412554003	40.0	39.0	41.0	35.0	41.0
26-27	38.87858722950554	40.0	38.0	41.0	35.0	41.0
28-29	38.73869408422095	40.0	38.0	41.0	35.0	41.0
30-31	38.53451025932737	40.0	38.0	41.0	34.5	41.0
32-33	38.22309937506596	40.0	37.5	41.0	34.0	41.0
34-35	38.13835893595813	40.0	38.0	41.0	34.0	41.0
36-37	38.027194203819995	40.0	37.5	41.0	33.5	41.0
38-39	37.84380371151833	40.0	37.0	41.0	33.0	41.0
40-41	37.58551331729562	40.0	37.0	41.0	33.0	41.0
42-43	37.29293773444058	40.0	36.5	41.0	33.0	41.0
44-45	36.909414044669404	40.0	36.0	41.0	31.5	41.0
46-47	36.493788719707055	39.0	35.5	41.0	31.0	41.0
48-49	36.138448409461716	39.0	35.0	41.0	30.0	41.0
50-51	35.841352898722185	39.0	35.0	41.0	29.5	41.0
52-53	35.69190750542711	39.0	35.0	41.0	29.0	41.0
54-55	35.66092948197745	39.0	35.0	41.0	29.0	41.0
56-57	35.469910583264024	39.0	35.0	41.0	28.0	41.0
58-59	35.33288091263748	38.5	35.0	41.0	28.0	41.0
60-61	34.98663096843747	38.0	35.0	40.0	28.0	41.0
62-63	34.605917985245064	37.0	35.0	40.0	28.0	41.0
64-65	34.39814373766755	37.0	35.0	39.5	28.0	41.0
66-67	34.025552458566736	36.0	34.5	39.0	27.0	41.0
68-69	33.68869368426729	35.5	34.0	39.0	26.0	41.0
70-71	33.28802836780977	35.0	34.0	38.0	26.0	40.5
72-73	32.89509996132061	35.0	34.0	37.0	26.0	39.0
74-75	32.549176882472196	35.0	34.0	37.0	25.5	39.0
76-77	31.126037460433615	34.0	31.5	35.0	23.0	37.0
78-79	31.860709430055717	35.0	33.0	36.0	24.5	37.0
80-81	31.828124883078065	35.0	33.0	35.0	24.5	37.0
82-83	31.678843470782354	35.0	33.5	35.0	24.0	36.5
84-85	31.499808409296573	35.0	33.0	35.0	24.0	36.0
86-87	31.3231464921871	35.0	33.0	35.0	23.0	36.0
88-89	31.17842971227334	35.0	33.0	35.0	21.5	35.5
90-91	31.043763251690322	35.0	33.0	35.0	20.0	35.0
92-93	30.867059145920898	35.0	33.0	35.0	19.0	35.0
94-95	30.786866824979505	35.0	33.0	35.0	17.0	35.0
96-97	30.685793310658973	35.0	33.0	35.0	6.0	35.0
98-99	30.5498765433373	35.0	33.0	35.0	2.0	35.0
100	30.22398282108731	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4657258201232679
1101	2	0.3615787480634083
1101	3	0.3897572268670828
1101	4	0.29473723296925414
1101	5	0.3273260615842233
1101	6	0.32237073795123194
1101	7	0.29947958570668476
1101	8	0.299033452557282
1101	9	0.42127105728017256
1101	10-11	0.4431045130670128
1101	12-13	0.4299266191608879
1101	14-15	0.5859890762647595
1101	16-17	0.5174734939516199
1101	18-19	0.5408540622829392
1101	20-21	0.5145883728166041
1101	22-23	0.537245121402961
1101	24-25	0.553314633514475
1101	26-27	0.5595214700672315
1101	28-29	0.5706902988553182
1101	30-31	0.6477265192356825
1101	32-33	0.7187983467159782
1101	34-35	0.6533199826828238
1101	36-37	0.7060027322742997
1101	38-39	0.7115807159698377
1101	40-41	0.7426604859298251
1101	42-43	0.7971017479238469
1101	44-45	0.9212319528222679
1101	46-47	0.9041708960995933
1101	48-49	0.9753364016411794
1101	50-51	1.0274180310385148
1101	52-53	1.0020497734207936
1101	54-55	1.14479317429862
1101	56-57	1.124330434991279
1101	58-59	1.1668631370894005
1101	60-61	1.218391512010065
1101	62-63	1.2545273022530523
1101	64-65	1.1787930955905566
1101	66-67	1.223483467629066
1101	68-69	1.2615299619227756
1101	70-71	1.2379994672686436
1101	72-73	1.225929988622731
1101	74-75	1.1982203081402858
1101	76-77	1.2225914832306088
1101	78-79	1.174989948270678
1101	80-81	1.1967347440234164
1101	82-83	1.1764032437803316
1101	84-85	1.2158721390702745
1101	86-87	1.1875241510517789
1101	88-89	1.1882978075005752
1101	90-91	1.1945372038975997
1101	92-93	1.2343889408780875
1101	94-95	1.2893185273547658
1101	96-97	1.3568153835188248
1101	98-99	1.3665221957941327
1101	100	1.4056395199464262
1102	1	0.3566452239408662
1102	2	0.3393914842570709
1102	3	0.3929664035285043
1102	4	0.2652531627774266
1102	5	0.2400097995467334
1102	6	0.2720612897311909
1102	7	0.2789781726867062
1102	8	0.30758031022490684
1102	9	0.36595982601984645
1102	10-11	0.36588654112544816
1102	12-13	0.38080373592951133
1102	14-15	0.44655904856451656
1102	16-17	0.4852200653466028
1102	18-19	0.4537844968871738
1102	20-21	0.4655602136005754
1102	22-23	0.5108473479942504
1102	24-25	0.5010108034492617
1102	26-27	0.5683655668626457
1102	28-29	0.5927835289827001
1102	30-31	0.5989058061063517
1102	32-33	0.6677744003271116
1102	34-35	0.6500294202914034
1102	36-37	0.6909065052980026
1102	38-39	0.702766920011193
1102	40-41	0.7710835583922631
1102	42-43	0.7666229769514317
1102	44-45	0.7714818891140851
1102	46-47	0.917580088855722
1102	48-49	0.8720284723584584
1102	50-51	0.8998767055295147
1102	52-53	0.9775833640063425
1102	54-55	1.0177944038863629
1102	56-57	1.090375425792054
1102	58-59	1.088372949136442
1102	60-61	1.1042347983505536
1102	62-63	0.9383930016466593
1102	64-65	1.0259858031924622
1102	66-67	1.0746052987877732
1102	68-69	1.05474839796765
1102	70-71	1.0193108825529293
1102	72-73	1.0457935357447
1102	74-75	1.0557674452955297
1102	76-77	1.1123175419699507
1102	78-79	1.092666149732139
1102	80-81	1.0744108577135307
1102	82-83	1.0725297317511213
1102	84-85	1.1152806095372902
1102	86-87	1.1698609575003864
1102	88-89	1.1403233235171655
1102	90-91	1.1454775604109848
1102	92-93	1.1897601229233494
1102	94-95	1.2745528975470677
1102	96-97	1.282870863488025
1102	98-99	1.3066391624481213
1102	100	1.3345524255289192
1103	1	0.3612030295261377
1103	2	0.29747184158962625
1103	3	0.33190683046742464
1103	4	0.2435819664622585
1103	5	0.31145627850790447
1103	6	0.2790942338476867
1103	7	0.2748082157735823
1103	8	0.2602849290001572
1103	9	0.33865359381093185
1103	10-11	0.3329652584829006
1103	12-13	0.35466057111329263
1103	14-15	0.46070711999797
1103	16-17	0.4649723077455761
1103	18-19	0.4401410233002778
1103	20-21	0.4319109675777355
1103	22-23	0.44560858408375026
1103	24-25	0.4717347309806783
1103	26-27	0.5244712976980068
1103	28-29	0.49702947733924674
1103	30-31	0.4945399356501454
1103	32-33	0.5088057546656231
1103	34-35	0.502456771159359
1103	36-37	0.555984150444857
1103	38-39	0.5861587645146003
1103	40-41	0.5626834103504379
1103	42-43	0.5216982448558198
1103	44-45	0.5033645325311227
1103	46-47	0.6208664339441938
1103	48-49	0.6020426153401317
1103	50-51	0.6813524510490581
1103	52-53	0.61904500343325
1103	54-55	0.6790013977327547
1103	56-57	0.6936990755162284
1103	58-59	0.7141464226313516
1103	60-61	0.7966348228374116
1103	62-63	0.7359744130954979
1103	64-65	0.7808941481446823
1103	66-67	0.7145982053502848
1103	68-69	0.6550212531974111
1103	70-71	0.6139762285915964
1103	72-73	0.6796712533995546
1103	74-75	0.6963411676187619
1103	76-77	0.6861331618117603
1103	78-79	0.7224091185040749
1103	80-81	0.6888665021094411
1103	82-83	0.7556324781689305
1103	84-85	0.691179585184404
1103	86-87	0.7345790955054987
1103	88-89	0.7613663600108538
1103	90-91	0.7989791904633954
1103	92-93	0.7816791725642851
1103	94-95	0.8350890546764873
1103	96-97	0.9362165439133285
1103	98-99	0.8788314306059313
1103	100	0.9620727122885171
1104	1	0.2754953781167373
1104	2	0.1390272354497668
1104	3	0.2039119810087584
1104	4	0.1591887524732556
1104	5	0.23065706675413367
1104	6	0.2212019146700186
1104	7	0.17590649593724805
1104	8	0.1732740024513717
1104	9	0.23882944072850876
1104	10-11	0.24516720193061303
1104	12-13	0.29598758596493013
1104	14-15	0.36977006605565066
1104	16-17	0.3710390957720904
1104	18-19	0.30548828312425513
1104	20-21	0.2967962540138487
1104	22-23	0.33296511881729884
1104	24-25	0.3174029524206574
1104	26-27	0.34625450803813607
1104	28-29	0.3763088089443798
1104	30-31	0.38564046971168864
1104	32-33	0.4218548647921878
1104	34-35	0.39511056500426633
1104	36-37	0.4295471328903204
1104	38-39	0.5214613323311355
1104	40-41	0.48678409311626325
1104	42-43	0.4544565378315184
1104	44-45	0.507416598202667
1104	46-47	0.5653534148776274
1104	48-49	0.6096027273766467
1104	50-51	0.6005391445506021
1104	52-53	0.6847318425489561
1104	54-55	0.691085134516328
1104	56-57	0.6918293934444861
1104	58-59	0.7189644288915602
1104	60-61	0.7376774202459657
1104	62-63	0.733134148616287
1104	64-65	0.7401609351117244
1104	66-67	0.7521872497669548
1104	68-69	0.7130593441976245
1104	70-71	0.7321062703006902
1104	72-73	0.6962928988660835
1104	74-75	0.6818193868552882
1104	76-77	0.6702339114251465
1104	78-79	0.7764891718610301
1104	80-81	0.7508813922139481
1104	82-83	0.7555079903568149
1104	84-85	0.7336611456637208
1104	86-87	0.7505178868537108
1104	88-89	0.7379519304986388
1104	90-91	0.7499268933479435
1104	92-93	0.7996999185149356
1104	94-95	0.8446343184828109
1104	96-97	0.8595451373763687
1104	98-99	0.8035434216831696
1104	100	0.8423925009190611
1105	1	0.21923314106043534
1105	2	0.1485812066484442
1105	3	0.17905073313276887
1105	4	0.1397723712749368
1105	5	0.17515451703367546
1105	6	0.14535124468835647
1105	7	0.1661431460228755
1105	8	0.1464120582079147
1105	9	0.1954204197474212
1105	10-11	0.19334982339142925
1105	12-13	0.21667948476919463
1105	14-15	0.2189145526327465
1105	16-17	0.2768204682748845
1105	18-19	0.23850840222001324
1105	20-21	0.2938657831618485
1105	22-23	0.3378972494357626
1105	24-25	0.3185567643978118
1105	26-27	0.3332638092587743
1105	28-29	0.3803671744514574
1105	30-31	0.3765330433939553
1105	32-33	0.3948273481522122
1105	34-35	0.4423803413566674
1105	36-37	0.37658385735790034
1105	38-39	0.422814318550671
1105	40-41	0.43408031193764884
1105	42-43	0.5003302168036967
1105	44-45	0.49718718985508303
1105	46-47	0.49886719095437826
1105	48-49	0.47632569432518324
1105	50-51	0.4129519852764858
1105	52-53	0.40177643741965596
1105	54-55	0.4658519692904548
1105	56-57	0.43955197488229913
1105	58-59	0.4133176148936144
1105	60-61	0.523652287848499
1105	62-63	0.4781131019372964
1105	64-65	0.5043849924012491
1105	66-67	0.541421579534429
1105	68-69	0.5017166924158047
1105	70-71	0.45812556910943414
1105	72-73	0.47706626193406265
1105	74-75	0.4885373683084637
1105	76-77	0.4173271099642264
1105	78-79	0.5133880033298368
1105	80-81	0.48477137748867705
1105	82-83	0.5082589478620783
1105	84-85	0.5170649091309052
1105	86-87	0.4860931970001836
1105	88-89	0.5085209713733541
1105	90-91	0.5782426369045801
1105	92-93	0.5784997115088224
1105	94-95	0.5881957529635216
1105	96-97	0.6064592381525884
1105	98-99	0.6129446393590534
1105	100	0.6372775625070801
1106	1	0.2062954395527612
1106	2	0.13786647876225544
1106	3	0.1578770220861898
1106	4	0.11041507747085433
1106	5	0.19271748880371575
1106	6	0.1698679883186216
1106	7	0.1643294704490259
1106	8	0.1732093135860424
1106	9	0.19640208194544329
1106	10-11	0.1905688612772849
1106	12-13	0.21541708904774026
1106	14-15	0.2999741656812134
1106	16-17	0.24874014107497544
1106	18-19	0.23011287612347076
1106	20-21	0.27663834989187563
1106	22-23	0.300822516886889
1106	24-25	0.24323783066981264
1106	26-27	0.2989524863234294
1106	28-29	0.3367064916552849
1106	30-31	0.29734075729380294
1106	32-33	0.3194000290640062
1106	34-35	0.32767599219841514
1106	36-37	0.31955567638334514
1106	38-39	0.27859990316643035
1106	40-41	0.32845106703664584
1106	42-43	0.32198419195347583
1106	44-45	0.36441702261755893
1106	46-47	0.27971623433777637
1106	48-49	0.34752885239806375
1106	50-51	0.39143393285794303
1106	52-53	0.41263273891291163
1106	54-55	0.43518503531937114
1106	56-57	0.45993145746084707
1106	58-59	0.450240994838623
1106	60-61	0.4375185645574149
1106	62-63	0.36353786748274786
1106	64-65	0.41316769932979724
1106	66-67	0.33827589456024754
1106	68-69	0.37285177021332316
1106	70-71	0.35080095497337993
1106	72-73	0.3851096696052778
1106	74-75	0.37413879471446876
1106	76-77	0.28927941728213824
1106	78-79	0.34454934634140244
1106	80-81	0.35353047852235875
1106	82-83	0.36329873755294884
1106	84-85	0.38704865647371633
1106	86-87	0.3794103406215932
1106	88-89	0.37453790682104326
1106	90-91	0.41462360376091567
1106	92-93	0.39732949108491056
1106	94-95	0.4431811813708961
1106	96-97	0.47320803656057464
1106	98-99	0.4597044583947252
1106	100	0.4968757667780075
1107	1	0.12083439071248847
1107	2	0.046305622762361054
1107	3	0.0904780217306822
1107	4	0.08315386483425158
1107	5	0.1793093932183183
1107	6	0.12634522751039867
1107	7	0.08270782780082442
1107	8	0.08281897068388844
1107	9	0.0744225445368798
1107	10-11	0.12314502841928743
1107	12-13	0.17207154123781976
1107	14-15	0.14646360479200382
1107	16-17	0.21450466848672534
1107	18-19	0.19187141475490677
1107	20-21	0.22997079047389235
1107	22-23	0.17697909449882587
1107	24-25	0.1844706894298156
1107	26-27	0.21966098554955948
1107	28-29	0.26416891527163955
1107	30-31	0.33048786547863784
1107	32-33	0.28803659535217463
1107	34-35	0.27127575915545066
1107	36-37	0.319935535805314
1107	38-39	0.2816035441495899
1107	40-41	0.2714719590763295
1107	42-43	0.24720286270465408
1107	44-45	0.2782278583976563
1107	46-47	0.32904581432384106
1107	48-49	0.24609677196689717
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2316	96-97	-0.8247569449651344
2316	98-99	-0.8296658569558808
2316	100	-0.9459317292031919
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37.0
3	53.0
4	61.0
5	169.0
6	397.0
7	968.0
8	1986.0
9	3675.0
10	6254.0
11	11734.0
12	19221.0
13	27376.0
14	32071.0
15	43022.0
16	58241.0
17	78874.0
18	79247.0
19	48634.0
20	32150.0
21	27096.0
22	28780.0
23	31645.0
24	35346.0
25	39109.0
26	45968.0
27	57307.0
28	71212.0
29	89814.0
30	112027.0
31	140084.0
32	178985.0
33	233473.0
34	334034.0
35	542187.0
36	1054562.0
37	2090852.0
38	1864903.0
39	329039.0
40	305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.374213155688544	18.350170522176914	13.613506460799767	23.662109861334777
2	14.945739524414309	22.83912764966976	35.79333692565368	26.421795900262246
3	19.90639148840837	27.619995591614156	29.319714828023912	23.153898091953558
4	13.982298943858918	19.011791027155425	33.447177140232384	33.55873288875327
5	13.558416069983117	37.42761161351885	32.83560175866074	16.178370557837297
6	31.456329318228676	33.47817246466151	17.923484478830712	17.1420137382791
7	25.110432365385275	32.8777388116835	22.144040600198842	19.867788222732386
8	28.420861685962066	30.778975545801273	21.22817252917017	19.57199023906649
9	28.60356309681794	14.35322720025473	19.453513644483518	37.58969605844381
10-11	25.131804218612313	26.160873138439584	25.181843633440803	23.525479009507293
12-13	27.85658384256557	24.110107956718227	25.761203496272167	22.272104704444036
14-15	26.526580968379758	23.2836049661235	24.84651248062396	25.34330158487278
16-17	23.214497672003873	26.421633591678457	27.621188944934193	22.742679791383473
18-19	24.877104562593907	25.18888779080824	29.08727479061136	20.846732855986495
20-21	24.614221543057056	25.05216953531375	28.112373708050843	22.22123521357835
22-23	23.143990062599045	26.026958845006153	25.711025102517347	25.118025989877452
24-25	23.4068216837833	26.969982490936793	25.095173518545018	24.528022306734893
26-27	24.534685235713656	25.003775389697303	25.8949608852855	24.566578489303538
28-29	23.714676472485646	24.243687504031996	28.61074769369718	23.430888329785173
30-31	25.45772944725143	24.72258967992968	27.25812097302658	22.561559899792314
32-33	21.981074838476193	25.968989622674066	27.947909842767693	24.10202569608205
34-35	21.680929993601918	26.741122132104927	28.303265110802638	23.27468276349051
36-37	24.280299765643587	26.31162306653379	26.506911475619653	22.901165692202966
38-39	24.937251239476314	24.731898110734445	26.28296837973401	24.047882270055236
40-41	23.303253501339665	26.19212240667728	26.483872220471156	24.0207518715119
42-43	25.896731625842012	25.097047830008908	26.19407940339752	22.81214114075156
44-45	25.629569526471958	24.810397127600424	26.469558939275412	23.090474406652206
46-47	23.793092770736138	25.500476084802443	28.379982762891704	22.326448381569712
48-49	25.01168897420091	26.127927952569085	27.161969961142546	21.698413112087458
50-51	23.13044091945588	27.026788863747264	25.9365600112918	23.90621020550506
52-53	22.646162905793744	27.35563378714292	24.046564087649973	25.95163921941337
54-55	23.748372859555456	26.784422085134672	23.786960991985335	25.680244063324537
56-57	24.69564180512842	24.80343620624619	24.50521105209426	25.995710936531125
58-59	24.01076729737661	24.667601603028274	26.727756717978473	24.59387438161664
60-61	24.543767932580113	25.538768635575	25.589542997832172	24.327920434012718
62-63	21.774925520379956	26.187401491392336	26.48986174618718	25.547811242040524
64-65	21.429456639594633	26.39692174755752	26.475440564365204	25.698181048482642
66-67	23.976277532357614	25.944428245566954	25.115722343785478	24.96357187828995
68-69	24.45552892167355	24.757092158091464	25.030045367052782	25.757333553182203
70-71	22.833355492126145	25.748620461938902	25.45077016158243	25.96725388435252
72-73	24.61190577930811	24.99172991155521	25.478903920408978	24.9174603887277
74-75	24.81885336525218	24.400363641571516	25.687056648941763	25.09372634423454
76-77	23.001531452841704	25.48647173650238	27.22314183936905	24.28885497128687
78-79	23.43597616398735	25.441706416362813	26.771912241753842	24.35040517789599
80-81	22.18568093658309	25.66507777550421	26.451444722921135	25.697796564991567
82-83	22.044399270499273	26.174054342011104	24.600470881609922	27.181075505879697
84-85	22.578927150049395	25.94726610692504	24.302385626954816	27.171421116070753
86-87	23.078022212806758	24.335652994693223	25.149970764474165	27.43635402802585
88-89	22.99088485733935	23.798327250916298	26.389454325491634	26.821333566252715
90-91	23.65714846021677	24.60025177891837	25.563120678093586	26.179479082771273
92-93	21.695837430477567	25.202354252459948	26.13536454923414	26.96644376782834
94-95	20.927132640275133	25.543288097385965	26.672511678842664	26.857067583496242
96-97	22.859436235954746	24.931296550735595	25.454133654036514	26.755133559273137
98-99	23.174024480776293	24.225625211427115	25.29516579885324	27.305184508943352
100	22.509200621610447	25.218586126527015	25.952249015751207	26.31996423611134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	701.0
1	474.0
2	288.5
3	331.0
4	291.5
5	341.5
6	617.5
7	1265.5
8	2021.5
9	2161.0
10	1803.5
11	1640.5
12	1829.0
13	2184.5
14	2601.5
15	3031.0
16	3617.0
17	4308.0
18	5033.5
19	5925.0
20	7038.0
21	8248.0
22	9884.0
23	11832.5
24	14174.0
25	17553.5
26	22026.5
27	27140.5
28	32848.5
29	40963.0
30	50392.0
31	59158.0
32	68754.0
33	79186.0
34	89432.5
35	97551.0
36	104051.5
37	113382.0
38	120350.0
39	124485.5
40	125944.5
41	126326.0
42	131639.0
43	146513.5
44	168309.0
45	188840.5
46	218434.0
47	263206.5
48	376324.0
49	531647.5
50	788190.0
51	769583.0
52	452212.0
53	320060.5
54	278687.5
55	245198.5
56	221004.0
57	197802.0
58	184933.5
59	171234.5
60	145418.5
61	118533.5
62	95668.5
63	75213.0
64	55994.0
65	44834.5
66	35412.5
67	25696.5
68	19425.0
69	15780.0
70	13407.0
71	11285.0
72	9711.0
73	10465.0
74	8420.0
75	6212.5
76	4748.0
77	3094.5
78	2284.5
79	1434.5
80	929.0
81	680.5
82	534.0
83	396.5
84	261.5
85	163.0
86	108.5
87	56.5
88	25.5
89	16.5
90	11.5
91	7.5
92	3.5
93	3.0
94	2.5
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.06120581124922557
3	0.026190513666932525
4	0.006618587936520388
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.032138469632809E-4
12-13	1.8062423218574158E-4
14-15	3.0319067545463765E-4
16-17	3.354450026306629E-4
18-19	0.0
20-21	0.002657756559304483
22-23	0.004909108596191048
24-25	7.095951978725562E-4
26-27	5.483235619924298E-4
28-29	0.0051348888864232245
30-31	0.002515837519729972
32-33	0.0013611326068282668
34-35	0.0017417336675053653
36-37	0.003999536569827135
38-39	0.004470449746597104
40-41	0.0010192367387623989
42-43	0.0027029126173509187
44-45	3.41895868065868E-4
46-47	0.002296508094933
48-49	5.160692348164045E-5
50-51	0.0018062423218574157
52-53	0.007063697651549537
54-55	0.014269314342673584
56-57	0.02434556615246388
58-59	0.026629172516526474
60-61	0.0246229533661777
62-63	0.008044229197700705
64-65	0.0067927613032709245
66-67	2.3223115566738204E-4
68-69	0.0015353059735788033
70-71	3.1609240632504774E-4
72-73	9.547280844103484E-4
74-75	0.005276807925997736
76-77	0.00113535231659609
78-79	0.0013546817413930617
80-81	0.0
82-83	0.0015159533772731882
84-85	6.450865435205056E-6
86-87	6.96693467002146E-4
88-89	5.805778891684551E-5
90-91	6.515374089557107E-4
92-93	9.353754881047332E-4
94-95	0.0016772250131533147
96-97	1.741733667505365E-4
98-99	0.0
100	8.128090448358371E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7750898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.341898319981176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53699739797307	25.0769368588189
2	12.399793650871684	8.020657308904804
3	3.7316946727394065	3.6207026900085997
4	1.717675872863465	2.222115937073404
5	0.9707635744693333	1.5698168409114328
6	0.6309310581445643	1.224330487765778
7	0.4289993188488168	0.9712256644684731
8	0.31525029665489973	0.8156634431805334
9	0.25147092634416857	0.7319742417229116
>10	1.6309041684085743	10.314504209746215
>50	0.18557635264506867	4.2179367090919095
>100	0.1627435369743119	10.837123248921339
>500	0.022808556908464096	5.05723939015981
>1k	0.012065669811867111	7.056140807374067
>5k	6.413645082688791E-4	1.3619558867990869
>10k+	0.0016835818342058075	16.901676275052864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	156156	2.0146826857997615	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	145668	1.8793693324309004	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	68732	0.8867617661850279	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	64841	0.8365611313682622	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	57865	0.7465586568162812	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	52241	0.6739993224010946	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	51234	0.6610072794145917	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	46486	0.5997498612418846	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	43626	0.5628509109525116	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	41912	0.5407373442406286	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	40231	0.5190495346474693	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	38274	0.49380084733407664	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	31583	0.40747536608016255	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25669	0.3311745297125572	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	23933	0.3087771249215252	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	23562	0.3039905827686031	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	21435	0.27654860120724073	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	21157	0.2729619200252667	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	20741	0.26759479998317615	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	20061	0.2588216229912973	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	18965	0.24468132595732778	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18892	0.24373949960378782	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	17930	0.23132803450645328	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	17013	0.21949714729828726	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	15498	0.19995102502961593	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15061	0.19431296863924669	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	14885	0.19204226400605454	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	14785	0.19075209091901352	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	14437	0.1862622885761108	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	14367	0.18535916741518207	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	14196	0.18315297143634196	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	14101	0.18192730700365298	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	14075	0.18159186200102234	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	13691	0.17663759734678486	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	13227	0.17065119422291455	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	12534	0.16171029472972037	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	12244	0.15796879277730141	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	11004	0.14197064649799288	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	10731	0.1384484739703709	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	10472	0.13510692567493468	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	10176	0.1312880133372933	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	10014	0.12919793293628687	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	9041	0.11664454879937783	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	8747	0.11285143992347726	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8419	0.10861967219798273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0037027967598077024	0.0	0.0	5.160692348164045E-5	0.0
2	0.006463767166075466	0.0	0.0	6.450865435205056E-5	0.0
3	0.006825015630446949	0.0	0.0	6.450865435205056E-5	0.0
4	0.0077152350605052475	0.0	0.0	6.450865435205056E-5	0.0
5	0.030409379661556636	0.0	0.0	3.4834673350107306E-4	0.0
6	0.06241857395104412	0.0	0.0	6.063813509092753E-4	0.0
7	0.06956613285325132	0.0	0.0	7.353986596133765E-4	0.0
8	0.07876506696385374	0.0	0.0	8.12809044835837E-4	0.0
9	0.08619646394520997	0.0	0.0	9.418263535399382E-4	0.0
10-11	0.15367251639745483	0.0	0.0	0.0016643232822829046	0.0
12-13	0.27209750405694927	0.0	0.0	0.003560877720233191	0.0
14-15	0.4161646818213838	0.0	0.0	0.005973501392999882	0.0
16-17	0.48356977475384144	0.0	0.0	0.007250772749170483	1.2901730870410112E-5
18-19	0.5158241019298667	0.0	0.0	0.008031327466830294	1.2901730870410112E-5
20-21	0.6311591250458979	0.0	0.0	0.010173014791318373	1.2901730870410112E-5
22-23	0.6632199262588669	0.0	0.0	0.012585638464085065	1.2901730870410112E-5
24-25	0.8089191729784084	0.0	0.0	0.02381014432134186	1.2901730870410112E-5
26-27	1.1185736155991215	0.0	0.0	0.06383131348135404	1.2901730870410112E-5
28-29	1.1895524879826826	0.0	0.0	0.09731775595550349	1.2901730870410112E-5
30-31	1.2095179165046424	0.0	0.0	0.17699884581115632	1.2901730870410112E-5
32-33	1.2285544204039325	0.0	0.0	0.3035003169955275	1.2901730870410112E-5
34-35	1.2420883360869928	0.0	0.0	0.4692101482950749	1.2901730870410112E-5
36-37	1.2651437291524155	0.0	0.0	0.7127238676086306	1.2901730870410112E-5
38-39	1.2886119776056917	0.0	0.0	1.0584321971467048	2.5803461740820224E-5
40-41	1.309983694792526	0.0	0.0	1.5483689760850936	2.5803461740820224E-5
42-43	1.354056007445847	0.0	0.0	1.9047728405147377	2.5803461740820224E-5
44-45	1.404992040922226	0.0	0.0	2.2682662060576724	2.5803461740820224E-5
46-47	1.4254864404098724	0.0	0.0	2.6881594881006046	2.5803461740820224E-5
48-49	1.439594483116666	0.0	0.0	3.065367393558785	2.5803461740820224E-5
50-51	1.4635207430158415	0.0	0.0	3.4801902953696464	2.5803461740820224E-5
52-53	1.47101664865155	0.0	0.0	3.9753329227142453	3.225432717602528E-5
54-55	1.495968596154923	0.0	0.0	4.4102631720866405	3.870519261123034E-5
56-57	1.5441952661485159	0.0	0.0	4.87112590050856	3.870519261123034E-5
58-59	1.5603611349291397	0.0	0.0	5.3502574798429805	3.870519261123034E-5
60-61	1.5714050165542108	0.0	0.0	5.779556123690442	3.870519261123034E-5
62-63	1.5799072571978112	1.935259630561517E-5	0.0	6.228117051727425	3.870519261123034E-5
64-65	1.58663550984673	3.225432717602528E-5	0.0	6.725078823124752	3.870519261123034E-5
66-67	1.5972278308913368	3.870519261123034E-5	0.0	7.220369820374362	4.5156058046435394E-5
68-69	1.6082652616509727	3.870519261123034E-5	0.0	7.808043661521542	5.160692348164045E-5
70-71	1.6222055818564507	3.870519261123034E-5	0.0	8.531424358829131	5.160692348164045E-5
72-73	1.657233781169614	3.870519261123034E-5	0.0	9.11602113716372	6.450865435205056E-5
74-75	1.6918878302875355	3.870519261123034E-5	0.0	9.666925045330231	6.450865435205056E-5
76-77	1.702654324698893	3.870519261123034E-5	0.0	10.28161511092005	6.450865435205056E-5
78-79	1.7106017909150655	3.870519261123034E-5	0.0	10.8761913780829	6.450865435205056E-5
80-81	1.726316099115225	3.870519261123034E-5	0.0	11.543236667544845	6.450865435205056E-5
82-83	1.7320831728142982	3.870519261123034E-5	0.0	12.32597822858719	6.450865435205056E-5
84-85	1.7527969017267417	3.870519261123034E-5	0.0	13.046217354427835	6.450865435205056E-5
86-87	1.7885282453723428	3.870519261123034E-5	0.0	13.751019559282035	6.450865435205056E-5
88	1.797288520633351	3.870519261123034E-5	0.0	14.324920802725051	6.450865435205056E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	2195	0.0	34.47674	3
CGATCTC	2225	0.0	29.145145	5
GATCTAC	3415	0.0	26.444082	1
TCTCGCG	1140	0.0	24.733046	8
TCTACAC	4455	0.0	22.684309	3
ATCTACA	4290	0.0	21.598667	2
CCGATCT	3515	0.0	21.52485	4
AGTACTC	2745	0.0	20.371443	5
AGTACTT	31415	0.0	20.239264	12-13
TACACTC	4765	0.0	20.2166	5
CGTTAAC	760	0.0	19.804066	1
CTCGCGC	1445	0.0	19.512575	9
GTACTAG	1845	0.0	18.609919	1
ATGTAAG	2550	0.0	18.444963	2
GAGTACT	30405	0.0	18.353657	12-13
TAGTACT	1950	0.0	18.074497	4
TACTTTT	35700	0.0	17.869797	14-15
GTATAAG	2215	0.0	17.624725	1
GATGTAA	2525	0.0	17.323656	1
CTACACT	6615	0.0	17.191948	4
>>END_MODULE