##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6693193 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3351370564094 34.0 31.0 34.0 31.0 34.0 2 32.382153629814646 34.0 31.0 34.0 31.0 34.0 3 32.4165644110367 34.0 31.0 34.0 31.0 34.0 4 35.860270128173504 37.0 35.0 37.0 35.0 37.0 5 35.87183605791735 37.0 37.0 37.0 35.0 37.0 6 35.96106208202871 37.0 37.0 37.0 35.0 37.0 7 35.9169099113084 37.0 37.0 37.0 35.0 37.0 8 35.94061309751564 37.0 37.0 37.0 35.0 37.0 9 37.6654378560427 39.0 38.0 39.0 35.0 39.0 10-11 37.593199314587224 39.0 38.0 39.0 35.0 39.0 12-13 37.572943511415254 39.0 38.0 39.0 35.0 39.0 14-15 38.99911551930446 41.0 39.0 41.0 36.0 41.0 16-17 38.954157156382614 41.0 39.0 41.0 36.0 41.0 18-19 38.932620350257345 41.0 39.0 41.0 36.0 41.0 20-21 38.9014317232448 41.0 39.0 41.0 35.0 41.0 22-23 38.8132629673162 41.0 39.0 41.0 35.0 41.0 24-25 38.740524365575595 41.0 39.0 41.0 35.0 41.0 26-27 38.624047446413094 40.5 38.0 41.0 35.0 41.0 28-29 38.488591319569004 40.0 38.0 41.0 34.0 41.0 30-31 38.28892473293389 40.0 38.0 41.0 34.0 41.0 32-33 38.079786881985925 40.0 38.0 41.0 34.0 41.0 34-35 37.93568129590764 40.0 38.0 41.0 33.5 41.0 36-37 37.7933463146812 40.0 38.0 41.0 33.0 41.0 38-39 37.6142063287283 40.0 37.5 41.0 33.0 41.0 40-41 37.398716800785515 40.0 37.0 41.0 33.0 41.0 42-43 37.16822397023364 40.0 37.0 41.0 32.0 41.0 44-45 36.85772664855175 40.0 36.0 41.0 31.0 41.0 46-47 36.49386152468635 40.0 36.0 41.0 30.5 41.0 48-49 36.1587681693924 39.0 35.0 41.0 30.0 41.0 50-51 34.89360743071356 38.0 34.0 40.0 27.5 40.5 52-53 34.83807847764139 38.0 34.5 39.5 27.5 40.5 54-55 35.695503102928605 39.0 35.0 40.5 28.5 41.0 56-57 35.72328005482585 39.0 35.0 41.0 29.0 41.0 58-59 35.51762678888835 39.0 35.0 41.0 29.0 41.0 60-61 35.18784218533665 38.0 35.0 40.5 28.0 41.0 62-63 34.84598479380469 37.0 35.0 40.0 28.0 41.0 64-65 34.53660016975456 37.0 35.0 40.0 28.0 41.0 66-67 34.22895940696765 36.0 35.0 39.0 27.0 41.0 68-69 33.99001082144203 36.0 35.0 39.0 27.0 41.0 70-71 33.66337561161018 35.0 34.0 39.0 26.5 41.0 72-73 33.30568907844133 35.0 34.0 37.5 26.0 39.5 74-75 32.951241208792275 35.0 34.0 37.0 26.0 39.0 76-77 32.60967523273271 35.0 34.0 36.5 26.0 39.0 78-79 32.28855420723711 35.0 34.0 36.0 25.0 38.0 80-81 31.985411671230757 35.0 34.0 35.5 24.0 37.0 82-83 31.737902522757075 35.0 33.5 35.0 24.0 37.0 84-85 31.500116312199573 35.0 33.0 35.0 23.0 36.0 86-87 31.27545761791121 35.0 33.0 35.0 20.0 36.0 88-89 31.064327294909916 35.0 33.0 35.0 19.5 36.0 90-91 30.846818924839013 35.0 33.0 35.0 17.5 35.5 92-93 30.612476063367666 35.0 33.0 35.0 9.0 35.0 94-95 30.325145786174105 35.0 33.0 35.0 2.0 35.0 96-97 30.047801848833583 35.0 32.0 35.0 2.0 35.0 98-99 29.76378852365381 35.0 32.0 35.0 2.0 35.0 100 29.22276139355312 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3452425244520967 1101 2 0.4407238526132957 1101 3 0.49789735274775637 1101 4 0.39116236338511357 1101 5 0.43876571449497703 1101 6 0.4380814336544958 1101 7 0.4095120947404354 1101 8 0.3927529800731975 1101 9 0.4831547995356047 1101 10-11 0.4567657428222631 1101 12-13 0.5073194037037538 1101 14-15 0.6901500423462892 1101 16-17 0.6630694855712633 1101 18-19 0.6625446490884954 1101 20-21 0.6356897182749819 1101 22-23 0.7121449685791532 1101 24-25 0.6177039008226899 1101 26-27 0.6701843117103223 1101 28-29 0.7522693110787912 1101 30-31 0.662938033726796 1101 32-33 0.7138379554980716 1101 34-35 0.8289877532021208 1101 36-37 0.8115172193505842 1101 38-39 0.8262837325189025 1101 40-41 0.8062418758085386 1101 42-43 0.699906003003214 1101 44-45 0.7205366330864607 1101 46-47 0.7640779303485346 1101 48-49 0.725700643788052 1101 50-51 0.6929342390519295 1101 52-53 0.689268351980175 1101 54-55 0.891162259914033 1101 56-57 0.9329173124946237 1101 58-59 0.9365927142182287 1101 60-61 0.9607936474707088 1101 62-63 0.954441956536833 1101 64-65 0.9267931812723447 1101 66-67 0.9001577410350379 1101 68-69 0.8840187725749971 1101 70-71 0.8260292084200884 1101 72-73 0.7899911258256154 1101 74-75 0.7384865794219948 1101 76-77 0.7579334721785003 1101 78-79 0.7682569086176798 1101 80-81 0.7115913606713349 1101 82-83 0.6211515234685976 1101 84-85 0.6272243646243538 1101 86-87 0.6257293609537591 1101 88-89 0.6191814547656733 1101 90-91 0.6482979293373106 1101 92-93 0.655348094477695 1101 94-95 0.5791056920001232 1101 96-97 0.5769259934103168 1101 98-99 0.548984649655349 1101 100 0.5413561713672586 1102 1 0.4976428804584714 1102 2 0.5936443664602677 1102 3 0.6012175834679354 1102 4 0.5021660582300598 1102 5 0.5051498530719201 1102 6 0.46830720227391254 1102 7 0.5187300819709932 1102 8 0.4891898844960565 1102 9 0.4567644765405774 1102 10-11 0.5367019329620817 1102 12-13 0.5807924889840663 1102 14-15 0.7251568446314138 1102 16-17 0.7097614828710448 1102 18-19 0.7121939305091161 1102 20-21 0.7198177052670118 1102 22-23 0.7422992514179967 1102 24-25 0.7347346731881146 1102 26-27 0.7512588141190051 1102 28-29 0.7171126760344606 1102 30-31 0.6710814854999754 1102 32-33 0.6980419688750388 1102 34-35 0.7492599135785696 1102 36-37 0.7452472424875438 1102 38-39 0.8188628259671233 1102 40-41 0.7983140308971883 1102 42-43 0.7472637879699562 1102 44-45 0.6952774019372256 1102 46-47 0.6382766070784882 1102 48-49 0.6626895563794122 1102 50-51 0.6567163455072702 1102 52-53 0.6602075501266853 1102 54-55 0.7956520482959988 1102 56-57 0.79735103159733 1102 58-59 0.7685395227624952 1102 60-61 0.7793394226538908 1102 62-63 0.8135929123771604 1102 64-65 0.7903064580856949 1102 66-67 0.7156236285953028 1102 68-69 0.7647344019961579 1102 70-71 0.7339663716286609 1102 72-73 0.6939395261678314 1102 74-75 0.6424540186906924 1102 76-77 0.6513733909304236 1102 78-79 0.6335008597711251 1102 80-81 0.5711243882514836 1102 82-83 0.5435136191356875 1102 84-85 0.5118702391548027 1102 86-87 0.5136763338793955 1102 88-89 0.5214756956987614 1102 90-91 0.5656211036910577 1102 92-93 0.5464110571415723 1102 94-95 0.4345216415338946 1102 96-97 0.5479695464274776 1102 98-99 0.5531970301016678 1102 100 0.6164179652565807 1103 1 0.4042521434227879 1103 2 0.5298934905685826 1103 3 0.5360306268496018 1103 4 0.3563970324860364 1103 5 0.42554566138586125 1103 6 0.41131164739440607 1103 7 0.4630531861979108 1103 8 0.43897646746421515 1103 9 0.5064126614667472 1103 10-11 0.4976688873969053 1103 12-13 0.4808677464972462 1103 14-15 0.6398132197667081 1103 16-17 0.6492748326120577 1103 18-19 0.659320739998094 1103 20-21 0.6334896435715294 1103 22-23 0.6858805822118015 1103 24-25 0.6075665132973498 1103 26-27 0.6195041916393862 1103 28-29 0.6012244199820032 1103 30-31 0.6254757766693402 1103 32-33 0.5724031864016226 1103 34-35 0.5852170033706017 1103 36-37 0.5288167070872092 1103 38-39 0.5363581124056864 1103 40-41 0.5439936069908313 1103 42-43 0.6027472140752295 1103 44-45 0.5504208130577766 1103 46-47 0.5467921483717078 1103 48-49 0.535817744669437 1103 50-51 0.46785493109672416 1103 52-53 0.41639658271564883 1103 54-55 0.5605498908049142 1103 56-57 0.5839094999995922 1103 58-59 0.5299078676383147 1103 60-61 0.5192476886316513 1103 62-63 0.5182163051900304 1103 64-65 0.5226990588743945 1103 66-67 0.4985327925111207 1103 68-69 0.4649126572839819 1103 70-71 0.3933863592554374 1103 72-73 0.40833273171535467 1103 74-75 0.3771960570891366 1103 76-77 0.40188093446876394 1103 78-79 0.4100634464487882 1103 80-81 0.3848426205238482 1103 82-83 0.2834525523967244 1103 84-85 0.34186087268499676 1103 86-87 0.41279870778881644 1103 88-89 0.41407150949192584 1103 90-91 0.40385270069732826 1103 92-93 0.4525889166169925 1103 94-95 0.3347125487798088 1103 96-97 0.4173523223727109 1103 98-99 0.42598227297768787 1103 100 0.3991306541108237 1104 1 0.3278544822835485 1104 2 0.43835266931689176 1104 3 0.4788733475890794 1104 4 0.38209404437487393 1104 5 0.4027941907641832 1104 6 0.3997961745959202 1104 7 0.40196210079621864 1104 8 0.38831407117262984 1104 9 0.46414885136356787 1104 10-11 0.5006391376648267 1104 12-13 0.494576721983087 1104 14-15 0.5977527593476282 1104 16-17 0.5726902045857614 1104 18-19 0.5247815947398422 1104 20-21 0.5483998539491566 1104 22-23 0.5181777710348499 1104 24-25 0.5561958187495435 1104 26-27 0.48169876388710264 1104 28-29 0.5471973175386466 1104 30-31 0.5880575158042731 1104 32-33 0.6129709091576245 1104 34-35 0.568682146059686 1104 36-37 0.5380971571957573 1104 38-39 0.6221974665358729 1104 40-41 0.5576066328909093 1104 42-43 0.6212469603084045 1104 44-45 0.580838655582653 1104 46-47 0.5886326701369882 1104 48-49 0.5808581137527611 1104 50-51 0.5451273035506219 1104 52-53 0.556205564694352 1104 54-55 0.5322035601882718 1104 56-57 0.4859562331255489 1104 58-59 0.46602896305714125 1104 60-61 0.47225725919841466 1104 62-63 0.4966552120904808 1104 64-65 0.5000749870994099 1104 66-67 0.483932913226802 1104 68-69 0.42377505742623356 1104 70-71 0.41483689622896947 1104 72-73 0.41522652706957786 1104 74-75 0.4324391976215267 1104 76-77 0.4038932113847835 1104 78-79 0.39758121417104775 1104 80-81 0.4659970814992178 1104 82-83 0.43498455960845206 1104 84-85 0.4309299057317091 1104 86-87 0.4397405426424221 1104 88-89 0.45527337178298666 1104 90-91 0.47437942078555295 1104 92-93 0.49037663889530947 1104 94-95 0.31708228024752927 1104 96-97 0.29570653354293697 1104 98-99 0.24920984702991333 1104 100 0.2631754515609295 1105 1 0.2990720762232897 1105 2 0.3304305877877667 1105 3 0.37166345828476466 1105 4 0.34906266894114424 1105 5 0.3521795429668799 1105 6 0.34175707508193653 1105 7 0.31149320941636205 1105 8 0.3129744392636482 1105 9 0.3171927995922843 1105 10-11 0.38635392765644383 1105 12-13 0.3113284935526295 1105 14-15 0.4379835520222244 1105 16-17 0.5044631602550069 1105 18-19 0.4937290687824927 1105 20-21 0.4604514670332449 1105 22-23 0.5143965978690588 1105 24-25 0.4920097817095126 1105 26-27 0.4171976027546975 1105 28-29 0.48138541079102026 1105 30-31 0.466366437252681 1105 32-33 0.4466862459023915 1105 34-35 0.42170887541282553 1105 36-37 0.413638909120273 1105 38-39 0.4387553067494778 1105 40-41 0.4860152155827109 1105 42-43 0.5039735645166488 1105 44-45 0.4431587955234235 1105 46-47 0.3603076083264014 1105 48-49 0.40988480381412984 1105 50-51 0.4110188020015144 1105 52-53 0.45637553610699655 1105 54-55 0.4946136202854561 1105 56-57 0.4837351705086661 1105 58-59 0.5334658204064198 1105 60-61 0.5309110251790443 1105 62-63 0.4799642623573348 1105 64-65 0.4925670547124099 1105 66-67 0.3887158630531218 1105 68-69 0.34639841386732684 1105 70-71 0.3943679183957087 1105 72-73 0.3431210135913645 1105 74-75 0.43832233737822435 1105 76-77 0.44516415626563344 1105 78-79 0.45182406143721465 1105 80-81 0.39768549378188567 1105 82-83 0.4055768599189733 1105 84-85 0.3541449940737351 1105 86-87 0.3447602340566185 1105 88-89 0.35966850198052214 1105 90-91 0.4142810213022976 1105 92-93 0.4066654022247498 1105 94-95 0.43079866419863677 1105 96-97 0.40770453402793905 1105 98-99 0.42515317932264907 1105 100 0.5588794383237357 1106 1 0.28842037598291625 1106 2 0.2698631564671885 1106 3 0.29857621649413346 1106 4 0.30914142659912613 1106 5 0.2610982452281547 1106 6 0.2838776314125795 1106 7 0.2839776922599242 1106 8 0.29671729942846525 1106 9 0.3657937380328562 1106 10-11 0.38032610056315974 1106 12-13 0.40666915126156056 1106 14-15 0.4630101301179508 1106 16-17 0.42821429602536654 1106 18-19 0.45164918289206923 1106 20-21 0.4970655826542014 1106 22-23 0.5154361046446567 1106 24-25 0.5543200565085158 1106 26-27 0.5235438468669997 1106 28-29 0.532502588496925 1106 30-31 0.49906276229134505 1106 32-33 0.5424456079583919 1106 34-35 0.4842462514787371 1106 36-37 0.5409218755298824 1106 38-39 0.5575012166692588 1106 40-41 0.5322123762875037 1106 42-43 0.5578502014926201 1106 44-45 0.5503792930451041 1106 46-47 0.5756572460600751 1106 48-49 0.624604807476679 1106 50-51 0.4756621221879769 1106 52-53 0.5389450577678048 1106 54-55 0.6053005045226101 1106 56-57 0.6293692341303938 1106 58-59 0.632163888433773 1106 60-61 0.632510069978153 1106 62-63 0.5440601659167399 1106 64-65 0.5682700864661427 1106 66-67 0.5535080300805291 1106 68-69 0.53167892241364 1106 70-71 0.5357838495806035 1106 72-73 0.43795045252674925 1106 74-75 0.5196320496451605 1106 76-77 0.4866468831832691 1106 78-79 0.46981472466162444 1106 80-81 0.5074448179238757 1106 82-83 0.45480067338581165 1106 84-85 0.4219546099450717 1106 86-87 0.4794759848014216 1106 88-89 0.5598503150066492 1106 90-91 0.5309471651990876 1106 92-93 0.5841941047991597 1106 94-95 0.4691727189290553 1106 96-97 0.5578206628673748 1106 98-99 0.6436907100017173 1106 100 0.7535744646752995 1107 1 0.19851428111573455 1107 2 0.21225844412154515 1107 3 0.2544275126212625 1107 4 0.2744908334419023 1107 5 0.31459315755733996 1107 6 0.21979597550917163 1107 7 0.2814913476401131 1107 8 0.23107231610606505 1107 9 0.3152809624199193 1107 10-11 0.2989001602987216 1107 12-13 0.2872381096129075 1107 14-15 0.29969726842706734 1107 16-17 0.3042019244302523 1107 18-19 0.3244405345901029 1107 20-21 0.3349974755884233 1107 22-23 0.2733297477460752 1107 24-25 0.33114240892597735 1107 26-27 0.28655153269937017 1107 28-29 0.37884501416503014 1107 30-31 0.42577519654614804 1107 32-33 0.400045151407717 1107 34-35 0.32448199534621835 1107 36-37 0.3874272796637328 1107 38-39 0.40977837921943916 1107 40-41 0.43337727673878135 1107 42-43 0.3926522749584649 1107 44-45 0.46468743099767806 1107 46-47 0.4803797185225207 1107 48-49 0.5438787779696668 1107 50-51 0.5087926378750467 1107 52-53 0.5088831786946599 1107 54-55 0.5440564672137214 1107 56-57 0.5195170012726749 1107 58-59 0.5057142239900827 1107 60-61 0.5290398417661066 1107 62-63 0.5235897541561414 1107 64-65 0.510579401661218 1107 66-67 0.48407287441235525 1107 68-69 0.46496904096068903 1107 70-71 0.46700799191302167 1107 72-73 0.4405955561051016 1107 74-75 0.4220427595983409 1107 76-77 0.4592670283765088 1107 78-79 0.34250217099477 1107 80-81 0.399553601039095 1107 82-83 0.38977332043035773 1107 84-85 0.3847311722703566 1107 86-87 0.35210435803371 1107 88-89 0.3093339528125867 1107 90-91 0.3571820567261099 1107 92-93 0.25791279869289596 1107 94-95 0.2633936818627909 1107 96-97 0.23053405916981973 1107 98-99 0.24339239084240916 1107 100 0.26254830162088183 1108 1 0.24317379036390463 1108 2 0.22097353680199916 1108 3 0.29746840529475804 1108 4 0.29210086508888367 1108 5 0.2803955353040948 1108 6 0.24838656686561933 1108 7 0.2842089144490316 1108 8 0.27492897563519136 1108 9 0.3492351463301375 1108 10-11 0.35344575033861503 1108 12-13 0.37667089827365174 1108 14-15 0.46667063428508015 1108 16-17 0.433903389531622 1108 18-19 0.41272369809621523 1108 20-21 0.35152097732478893 1108 22-23 0.3763672620915486 1108 24-25 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44939.0 27 53368.0 28 64050.0 29 77743.0 30 95519.0 31 119133.0 32 153055.0 33 206769.0 34 304367.0 35 494349.0 36 922141.0 37 1691151.0 38 1592958.0 39 348718.0 40 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54692774162426 17.961048106006285 13.29542210645519 24.196602045914265 2 15.576798974298582 21.529780866748865 37.04523793363177 25.84818222532078 3 18.826967599269445 26.260119562512145 29.518592844199983 25.394319994018428 4 13.59879315646468 17.710401907548857 35.11097098336709 33.579833952619374 5 14.37315464650604 36.16284288088141 33.21369482001461 16.250307652597936 6 31.500359560684938 34.499731732558686 18.03728505866339 15.96262364809298 7 26.533625222191038 31.86773692581158 22.051843951726074 19.546793900271307 8 27.251861278889944 31.331569130490404 21.188330523926346 20.228239066693302 9 27.481795787647517 14.827701123647316 19.758445919139433 37.93205716956574 10-11 25.166251239785502 25.239333726710434 26.696356380184966 22.898058653319097 12-13 26.80581140208826 23.311402275469252 26.750299184120912 23.132487138321576 14-15 25.044529059780775 23.303639810547118 25.160926072743827 26.490905056928277 16-17 23.53443231829861 26.255026165239816 26.465837040761507 23.744704475700072 18-19 24.275186294072533 25.39036892422694 28.08895388551932 22.245490896181202 20-21 24.695584982881194 24.800597496348367 27.432400627380147 23.07141689339029 22-23 23.774757444874993 25.37057687763117 26.16768963839467 24.686976039099164 24-25 23.64552229226277 25.99355436013323 25.858083198709224 24.502840148894776 26-27 24.15818443473797 25.119721528857824 26.12229039310936 24.599803643294848 28-29 23.69652715208742 24.553841698463007 27.806779363056904 23.94285178639267 30-31 24.750019229722117 24.58888651632856 27.108028796574406 23.553065457374913 32-33 22.948319039218664 25.338554771378146 27.234831363339357 24.478294826063834 34-35 23.06782416695465 25.589624185494326 27.406699526834238 23.935852120716785 36-37 23.994689757021597 25.631820160968143 26.5074042652518 23.86608581675846 38-39 24.299939540742987 24.714097417483284 26.480152401602634 24.505810640171095 40-41 23.803325112073587 25.357215742671702 26.471546541824964 24.367912603429748 42-43 24.99061895734264 24.956295971690157 26.150756814728204 23.902328256239 44-45 24.67582062095554 24.799592523686357 26.58239864202489 23.94218821333321 46-47 24.003607336294436 24.90031395023834 27.292913880684537 23.803164832782684 48-49 24.231369413234376 25.611886973324914 26.741655174829877 23.415088438610834 50-51 23.459671857814435 26.049194377426097 25.811933461237974 24.67920030352149 52-53 23.313890597016933 26.135600929421148 25.102914091383106 25.447594382178814 54-55 23.907236055972746 25.542854852859882 25.062254494189055 25.48765459697832 56-57 24.250674819928967 24.793761910649344 25.365129021890503 25.590434247531185 58-59 24.08448980440698 24.697922070110774 26.56886841163842 24.648719713843825 60-61 23.93767775301491 25.09938013719582 26.07502196272985 24.88792014705942 62-63 22.719178112422593 25.411347380316386 26.466550558957554 25.402923948303467 64-65 22.95807610079422 25.27988131790614 26.339268285641758 25.42277429565788 66-67 23.94653482541278 25.22292675407502 25.652539190420526 25.177999230091675 68-69 24.108491862721433 24.662570732120255 25.709071090622963 25.51986631453535 70-71 23.465596170893647 25.03559978903053 25.943380384073045 25.555423656002773 72-73 24.348304474208675 24.783295633201725 25.864065992301555 25.004333900288046 74-75 24.549700239265196 24.52410985855383 26.05156892086809 24.874620981312884 76-77 23.914956942213152 25.2712570227641 26.360932283626724 24.452853751396024 78-79 23.991045571787936 25.170036304102943 26.309939177285145 24.52897894682397 80-81 23.56241368469762 25.23617152953782 26.016512713718708 25.18490207204585 82-83 23.66423195483533 25.622871403899953 25.208203583653425 25.504693057611288 84-85 23.85404722155724 25.4866662870116 25.138615881889457 25.5206706095417 86-87 23.97074031105484 24.73304567912073 25.651198049034736 25.645015960789692 88-89 24.143781979608182 24.324414525014753 26.123324309313794 25.40847918606327 90-91 24.48230105846127 24.8692151833412 25.640648792068234 25.0078349661293 92-93 23.87961630290078 25.022234784515003 25.889456826522007 25.20869208606221 94-95 23.64108270281184 25.12810800666888 26.06343684614721 25.167372444372067 96-97 24.563848490362574 24.91348796074057 25.33864997658149 25.184013572315365 98-99 24.652284919448377 24.541741299424988 25.299119244063768 25.506854537062868 100 24.67037921978756 24.75682236788493 25.510678655250768 25.062119757076744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2235.0 1 3051.5 2 2461.0 3 948.5 4 768.0 5 776.0 6 981.5 7 1577.5 8 2394.0 9 2660.5 10 2276.5 11 2036.5 12 2260.0 13 2505.0 14 2777.5 15 3098.5 16 3383.5 17 3855.5 18 4467.0 19 5094.5 20 5930.5 21 7090.5 22 8741.5 23 10736.5 24 12993.0 25 16083.5 26 20547.0 27 25816.0 28 30987.5 29 37741.5 30 45202.0 31 52227.5 32 59537.5 33 67064.0 34 75167.5 35 82161.5 36 89708.5 37 99080.0 38 106898.5 39 114805.0 40 122650.5 41 130278.5 42 140185.0 43 156279.5 44 175162.0 45 191922.5 46 216352.0 47 246790.0 48 329706.0 49 410477.0 50 447580.0 51 406404.0 52 315356.5 53 284017.5 54 265611.5 55 243728.0 56 225008.5 57 205625.0 58 193902.0 59 177883.0 60 151980.5 61 127236.0 62 105009.5 63 83465.5 64 63976.0 65 51216.5 66 40377.0 67 30740.5 68 24233.5 69 19848.5 70 17008.0 71 14402.0 72 12010.0 73 11494.0 74 9540.0 75 7551.0 76 5873.0 77 4038.0 78 2974.5 79 2188.5 80 1531.0 81 1166.5 82 923.0 83 679.5 84 464.5 85 308.5 86 229.0 87 159.5 88 119.0 89 98.5 90 80.0 91 66.5 92 59.5 93 48.5 94 37.0 95 33.5 96 33.0 97 23.0 98 18.0 99 15.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08689425211554484 2 0.28575300308836155 3 0.289278973428676 4 0.2975709799493306 5 0.324314568547478 6 0.17074063156403826 7 0.18777286117403158 8 0.15370840195404495 9 0.20461086360426184 10-11 0.22593850199747714 12-13 0.24329942375783875 14-15 0.16913452219292047 16-17 0.15931110906259538 18-19 0.1766122686137991 20-21 0.20900338597736537 22-23 0.17205540016551144 24-25 0.19681936558530433 26-27 0.1942794716960948 28-29 0.2243174520740699 30-31 0.2589123009003326 32-33 0.22726821115123977 34-35 0.1528194390928216 36-37 0.1476574782768105 38-39 0.11502731207661276 40-41 0.10566705606726116 42-43 0.12204190137651791 44-45 0.13631012881295967 46-47 0.20127912044371052 48-49 0.20638878932670848 50-51 0.21303733509552167 52-53 0.141382446315234 54-55 0.08551972130491381 56-57 0.12343137273943842 58-59 0.14298108541020707 60-61 0.11958418052490044 62-63 0.14128533272535246 64-65 0.11608809128916499 66-67 0.11924801809838742 68-69 0.10534583419303761 70-71 0.13167855760322464 72-73 0.15558344126637316 74-75 0.1934577413201741 76-77 0.1749090456527998 78-79 0.19974771383404005 80-81 0.10385177896409083 82-83 0.14418379986950922 84-85 0.1746475859877341 86-87 0.18828831022801823 88-89 0.17026253389077528 90-91 0.173414990423853 92-93 0.12377500544209619 94-95 0.3631824153285286 96-97 0.36340652361287057 98-99 0.4108427771319309 100 0.4460352480497724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6693193.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.39975879076647 #Duplication Level Percentage of deduplicated Percentage of total 1 78.82826499911945 34.99955951855523 2 11.693694135923746 10.383943980160293 3 3.52623684566693 4.696921961601747 4 1.6893867625330243 3.000334590431206 5 0.9478652332576144 2.1042493861395837 6 0.609357239064075 1.623318865911141 7 0.4172418206633448 1.2967805336410925 8 0.3202219790576697 1.1374222903729925 9 0.2447242674261903 0.9779128599572889 >10 1.4846623549551998 12.46893720501988 >50 0.12965962867373076 3.9891933792667937 >100 0.09135018387905597 8.163508622326749 >500 0.010170982806909062 3.072377416936737 >1k 0.006082273845203889 4.989861433484289 >5k 5.068561537246244E-4 1.575255359107294 >10k+ 5.74436974221241E-4 5.520422597087552 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 49840 0.7446371261070762 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 42676 0.6376030095053288 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 30556 0.4565235157569788 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 30224 0.4515632523968754 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 27333 0.40837011572802395 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 20749 0.3100015194541679 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19823 0.2961665680341206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18092 0.27030447202105184 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17839 0.2665245122918165 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 17409 0.2601000748073453 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 16451 0.24578702571403516 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14622 0.21846075557659847 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 14009 0.2093021970231547 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 13331 0.19917250257089555 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 12949 0.19346521159631883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12035 0.17980954680374522 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 10020 0.1497043339404676 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9440 0.14103881361257625 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 8821 0.13179061174539564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8746 0.13067007032368558 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7912 0.11820964971426941 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 7888 0.11785107645932219 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 7762 0.11596856687084924 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7368 0.11008198926879892 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6895 0.10301510803588064 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1952441831574258E-4 0.0 0.0 0.0 0.0 2 1.7928662747361386E-4 0.0 0.0 0.0 0.0 3 2.091677320525495E-4 0.0 0.0 0.0 0.0 4 2.689299412104208E-4 0.0 0.0 1.4940552289467822E-5 0.0 5 0.0011952441831574257 0.0 0.0 4.4821656868403464E-5 0.0 6 0.00233072615715698 0.0 0.0 4.4821656868403464E-5 0.0 7 0.002599656098367401 0.0 0.0 4.4821656868403464E-5 0.0 8 0.0028685860395778217 0.0 0.0 5.976220915787129E-5 0.0 9 0.0031673970853671784 0.0 0.0 1.1952441831574258E-4 2.9881104578935644E-5 10-11 0.005229193301313738 0.0 0.0 1.494055228946782E-4 2.9881104578935644E-5 12-13 0.010069932243101312 2.9881104578935644E-5 0.0 2.2410828434201731E-4 2.9881104578935644E-5 14-15 0.015485882448033398 2.9881104578935644E-5 0.0 2.764002173551547E-4 5.2291933013137376E-5 16-17 0.017293689275059004 2.9881104578935644E-5 0.0 3.884543595261634E-4 5.976220915787129E-5 18-19 0.018107949374835 2.9881104578935644E-5 0.0 4.7809767326297036E-4 5.976220915787129E-5 20-21 0.02267228809926742 2.9881104578935644E-5 0.0 0.0010234278318285459 5.976220915787129E-5 22-23 0.024412862440990418 2.9881104578935644E-5 0.0 0.0024128991947490533 8.217303759207302E-5 24-25 0.03464714075927588 2.9881104578935644E-5 0.0 0.005737172079155643 1.1205414217100866E-4 26-27 0.06726983668332887 2.9881104578935644E-5 0.0 0.0116312199573507 1.344649706052104E-4 28-29 0.07428442598323401 2.9881104578935644E-5 0.0 0.034505205512525935 1.344649706052104E-4 30-31 0.07571871900302293 2.9881104578935644E-5 0.0 0.0968596004926199 1.344649706052104E-4 32-33 0.07680937932015408 3.735138072366955E-5 0.0 0.1983507721949748 1.344649706052104E-4 34-35 0.07772075300981161 4.4821656868403464E-5 0.0 0.3336524137283954 1.344649706052104E-4 36-37 0.07892346746911377 5.976220915787128E-5 0.0 0.5321824725508438 1.344649706052104E-4 38-39 0.08028305772745534 7.47027614473391E-5 0.0 0.8122879468737865 1.494055228946782E-4 40-41 0.08167252909037584 7.47027614473391E-5 0.0 1.1754404810977364 1.494055228946782E-4 42-43 0.08486233700417722 7.47027614473391E-5 0.0 1.4552770254794685 1.7928662747361386E-4 44-45 0.08865723728570205 7.47027614473391E-5 0.0 1.756105045827903 1.867569036183478E-4 46-47 0.09046504411272766 7.47027614473391E-5 0.0 2.1027034481151223 2.016974559078156E-4 48-49 0.09164534774359562 7.47027614473391E-5 0.0 2.4209745632615105 2.2410828434201734E-4 50-51 0.09348303567520017 7.47027614473391E-5 0.0 2.75957229979772 2.2410828434201734E-4 52-53 0.09425247411810775 7.47027614473391E-5 0.0 3.1425061252529245 2.3157856048675125E-4 54-55 0.09591834569838342 7.47027614473391E-5 0.0 3.497030490529707 2.3904883663148515E-4 56-57 0.10037810055678956 7.47027614473391E-5 0.0 3.879426456102491 2.3904883663148515E-4 58-59 0.10192444771874948 7.47027614473391E-5 0.0 4.266902209453694 2.3904883663148515E-4 60-61 0.10271629699009127 7.47027614473391E-5 0.0 4.623712479230765 2.3904883663148515E-4 62-63 0.10341850294769626 7.47027614473391E-5 0.0 4.990428335175752 2.53989388920953E-4 64-65 0.10398624393469605 7.47027614473391E-5 0.0 5.389871470910819 2.53989388920953E-4 66-67 0.10476315265374836 8.964331373680693E-5 0.0 5.808797086831353 2.53989388920953E-4 68-69 0.10553259109665596 8.964331373680693E-5 0.0 6.292400353612992 2.53989388920953E-4 70-71 0.10648131616703717 8.964331373680693E-5 0.0 6.831560064083017 2.53989388920953E-4 72-73 0.10884939370491782 8.964331373680693E-5 0.0 7.302269634238845 2.689299412104208E-4 74-75 0.11114276848135113 8.964331373680693E-5 0.0 7.765411814660059 2.838704934998886E-4 76-77 0.11194955830498239 8.964331373680693E-5 0.0 8.273315889740516 2.988110457893564E-4 78-79 0.11250982901583743 8.964331373680693E-5 0.0 8.783177177170895 2.988110457893564E-4 80-81 0.11370507319899487 8.964331373680693E-5 0.0 9.345703911421648 2.988110457893564E-4 82-83 0.11420558170069203 8.964331373680693E-5 0.0 9.965094387686115 2.988110457893564E-4 84-85 0.11580422079566509 8.964331373680693E-5 0.0 10.560243817860922 2.988110457893564E-4 86-87 0.11932272085983477 1.0458386602627475E-4 0.0 11.155258783065122 2.988110457893564E-4 88 0.12015192151190023 1.0458386602627475E-4 0.0 11.622121161006413 2.988110457893564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 5295 0.0 25.428993 1 ATCTACA 6390 0.0 20.762875 2 TACACTC 6880 0.0 20.44618 5 TCTACAC 7050 0.0 19.95046 3 AGTACTT 22295 0.0 18.22437 12-13 CTACACT 8495 0.0 18.221525 4 GAGTACT 21410 0.0 18.18741 12-13 GTATATA 1530 0.0 18.154726 1 CATGGGG 12105 0.0 16.246645 4 TATCACG 380 4.449248E-9 16.095295 2 GTACTTT 24550 0.0 15.923136 14-15 GTACTAG 1670 0.0 15.505127 1 TACTTTT 26790 0.0 15.363429 14-15 CATGGGT 5845 0.0 15.294009 4 GTATTAA 2005 0.0 15.262584 1 CACTCTT 8750 0.0 15.258699 7 GTATAAG 2000 0.0 14.829948 1 GTCTTAG 2040 0.0 14.769945 1 AGTACTC 2540 0.0 14.632646 5 ACTCTTT 10965 0.0 14.620188 8 >>END_MODULE