##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765248_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6899359
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64338542174715	34.0	31.0	34.0	31.0	34.0
2	32.77255524752372	34.0	31.0	34.0	31.0	34.0
3	32.82724003780641	34.0	31.0	34.0	31.0	34.0
4	36.28672272308196	37.0	37.0	37.0	35.0	37.0
5	36.21945719884992	37.0	37.0	37.0	35.0	37.0
6	36.28552551041336	37.0	37.0	37.0	35.0	37.0
7	36.22825062444207	37.0	37.0	37.0	35.0	37.0
8	36.233107742327945	37.0	37.0	37.0	35.0	37.0
9	38.00575720150234	39.0	38.0	39.0	35.0	39.0
10-11	37.950187981231295	39.0	38.0	39.0	35.0	39.0
12-13	37.938101496095506	39.0	38.0	39.0	35.0	39.0
14-15	39.32573779680112	41.0	39.0	41.0	36.0	41.0
16-17	39.30052617931608	41.0	39.0	41.0	36.0	41.0
18-19	39.31884360561612	41.0	39.0	41.0	36.0	41.0
20-21	39.310184250449936	41.0	39.0	41.0	36.0	41.0
22-23	39.249647612191225	41.0	39.0	41.0	36.0	41.0
24-25	39.20061711530013	41.0	39.0	41.0	36.0	41.0
26-27	39.06094218317963	40.5	39.0	41.0	35.0	41.0
28-29	38.92922009131573	40.0	38.5	41.0	35.0	41.0
30-31	38.75456285141851	40.0	38.0	41.0	35.0	41.0
32-33	38.49478081949352	40.0	38.0	41.0	34.5	41.0
34-35	38.42209399452906	40.0	38.0	41.0	34.5	41.0
36-37	38.31326171025454	40.0	38.0	41.0	34.0	41.0
38-39	38.122659655773816	40.0	38.0	41.0	34.0	41.0
40-41	37.90866100749359	40.0	37.5	41.0	33.0	41.0
42-43	37.66889750192735	40.0	37.0	41.0	33.0	41.0
44-45	37.34993294304587	40.0	37.0	41.0	33.0	41.0
46-47	37.03399728873363	40.0	36.0	41.0	32.0	41.0
48-49	36.76923711318689	40.0	36.0	41.0	31.5	41.0
50-51	36.497276920942944	39.0	35.5	41.0	31.0	41.0
52-53	36.363206857332685	39.0	35.0	41.0	31.0	41.0
54-55	36.33785921271817	39.0	35.0	41.0	31.0	41.0
56-57	36.1580000402936	39.0	35.0	41.0	31.0	41.0
58-59	36.03079691896015	39.0	35.0	41.0	31.0	41.0
60-61	35.71929044422822	38.5	35.0	41.0	31.0	41.0
62-63	35.380707541091866	37.5	35.0	40.0	30.0	41.0
64-65	35.14676406895191	37.0	35.0	40.0	30.0	41.0
66-67	34.766116895786986	36.5	35.0	39.0	30.0	41.0
68-69	34.422806162137675	36.0	35.0	39.0	29.5	41.0
70-71	34.04429157839156	35.0	35.0	38.5	29.0	40.5
72-73	33.64672667417365	35.0	34.0	37.0	29.0	39.5
74-75	33.291894507881096	35.0	34.0	37.0	29.0	39.0
76-77	31.862907988988546	34.5	32.0	35.5	25.5	37.0
78-79	32.63509457907611	35.0	34.0	36.0	27.5	37.0
80-81	32.57115892940199	35.0	34.0	35.5	28.5	37.0
82-83	32.40288141260659	35.0	34.0	35.0	28.0	36.5
84-85	32.21941951418965	35.0	34.0	35.0	27.0	36.0
86-87	32.061615941422964	35.0	34.0	35.0	27.0	36.0
88-89	31.91874897363654	35.0	34.0	35.0	27.0	36.0
90-91	31.804082669129116	35.0	34.0	35.0	26.0	35.0
92-93	31.679386519820174	35.0	34.0	35.0	25.0	35.0
94-95	31.58986943569685	35.0	34.0	35.0	25.0	35.0
96-97	31.49074500979004	35.0	34.0	35.0	25.0	35.0
98-99	31.370291645934067	35.0	33.5	35.0	24.5	35.0
100	31.049638814272456	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3611048472055174
1101	2	0.29455005789837685
1101	3	0.2914775170840045
1101	4	0.17357601760279806
1101	5	0.22627425921767497
1101	6	0.1730473091701228
1101	7	0.20569841443971626
1101	8	0.24112305058094563
1101	9	0.27433364843445673
1101	10-11	0.29028407210950036
1101	12-13	0.2606468222073417
1101	14-15	0.44405995665886877
1101	16-17	0.39611639533476506
1101	18-19	0.38382035031141015
1101	20-21	0.4137256636911175
1101	22-23	0.42115362148618374
1101	24-25	0.42375657737683525
1101	26-27	0.43937695547072764
1101	28-29	0.47961239540547496
1101	30-31	0.4707451487164036
1101	32-33	0.4812067176764856
1101	34-35	0.5383409037863203
1101	36-37	0.51109421117296
1101	38-39	0.5404993403553817
1101	40-41	0.5587225868682779
1101	42-43	0.5205719452673705
1101	44-45	0.5516164091575462
1101	46-47	0.48136371148553536
1101	48-49	0.4415404096414832
1101	50-51	0.5096750078286831
1101	52-53	0.5896319794336264
1101	54-55	0.60164088923446
1101	56-57	0.5964263494977331
1101	58-59	0.5849197693843706
1101	60-61	0.5925904953230798
1101	62-63	0.5985856385609409
1101	64-65	0.5976761147310654
1101	66-67	0.6244160901421623
1101	68-69	0.6045892428822199
1101	70-71	0.5506170298000157
1101	72-73	0.5693535859382166
1101	74-75	0.5742930337345413
1101	76-77	0.5242801584246664
1101	78-79	0.580810500518048
1101	80-81	0.5197303261787667
1101	82-83	0.588336290001024
1101	84-85	0.5271538904726256
1101	86-87	0.5942168433391899
1101	88-89	0.6472732584417109
1101	90-91	0.6848944236974468
1101	92-93	0.6822018101921721
1101	94-95	0.7282646761586804
1101	96-97	0.7456356141729081
1101	98-99	0.7688948060172542
1101	100	0.7538740734531508
1102	1	0.331642453146749
1102	2	0.2831573668940237
1102	3	0.33485498802356517
1102	4	0.23685092446775968
1102	5	0.24823534147219561
1102	6	0.26800026076035266
1102	7	0.23727850838793785
1102	8	0.2666912025776469
1102	9	0.29046144495640647
1102	10-11	0.3055108850747814
1102	12-13	0.32645444170081106
1102	14-15	0.4006004510914849
1102	16-17	0.46510257548212053
1102	18-19	0.4333015111119636
1102	20-21	0.40446583892826027
1102	22-23	0.3851453276387602
1102	24-25	0.3893986212039806
1102	26-27	0.4431325191362063
1102	28-29	0.4412448647316083
1102	30-31	0.4935038447618467
1102	32-33	0.47086885497743936
1102	34-35	0.5135250710145414
1102	36-37	0.5579628741087248
1102	38-39	0.5692498360735598
1102	40-41	0.5944094222156835
1102	42-43	0.6331659204152729
1102	44-45	0.6694724268320371
1102	46-47	0.6859176523717991
1102	48-49	0.6824799968467801
1102	50-51	0.7463243237341857
1102	52-53	0.7062430270543345
1102	54-55	0.7756934898241781
1102	56-57	0.7737288437185015
1102	58-59	0.8215524534251557
1102	60-61	0.7896810914077435
1102	62-63	0.7583541598123062
1102	64-65	0.7572073251749956
1102	66-67	0.691275436507155
1102	68-69	0.6253609764010335
1102	70-71	0.6528246780857003
1102	72-73	0.6406504024220894
1102	74-75	0.6084688232743787
1102	76-77	0.5871262835478177
1102	78-79	0.6502268256748138
1102	80-81	0.6436994402606828
1102	82-83	0.666846768825593
1102	84-85	0.6486570579567328
1102	86-87	0.6943787041013039
1102	88-89	0.6994014418589991
1102	90-91	0.724597649883858
1102	92-93	0.7721452986603587
1102	94-95	0.7213086032309519
1102	96-97	0.7893215925702997
1102	98-99	0.8317453617601025
1102	100	0.7953347219840872
1103	1	0.32148133672072987
1103	2	0.2882162583446046
1103	3	0.31146382797255967
1103	4	0.18915560849687552
1103	5	0.2675926232640933
1103	6	0.23196246723523473
1103	7	0.2685638656649658
1103	8	0.2500043854466014
1103	9	0.29699663592857206
1103	10-11	0.331059713657325
1103	12-13	0.30456292079446
1103	14-15	0.4301393579375272
1103	16-17	0.4312287076307726
1103	18-19	0.41247417085551774
1103	20-21	0.43023625720583425
1103	22-23	0.44142343209977497
1103	24-25	0.4554091554648423
1103	26-27	0.4545772987461518
1103	28-29	0.44250470573650347
1103	30-31	0.4315144010272576
1103	32-33	0.4242367545445447
1103	34-35	0.4739157843474118
1103	36-37	0.47266938775840117
1103	38-39	0.5580431817427396
1103	40-41	0.47481716140949715
1103	42-43	0.5393971429566378
1103	44-45	0.5378537617220758
1103	46-47	0.5908393145595952
1103	48-49	0.4697182609089978
1103	50-51	0.40106533305723246
1103	52-53	0.45361903253205327
1103	54-55	0.5334674823615515
1103	56-57	0.5242482026088169
1103	58-59	0.5508797562674204
1103	60-61	0.6066751229626561
1103	62-63	0.6061201734477919
1103	64-65	0.6248322857743034
1103	66-67	0.6509571947145787
1103	68-69	0.5782843936770163
1103	70-71	0.5403989683793924
1103	72-73	0.5634861019631856
1103	74-75	0.5699459148311306
1103	76-77	0.5135687510713254
1103	78-79	0.5443630566258406
1103	80-81	0.5047133360063327
1103	82-83	0.5622484223453981
1103	84-85	0.5681708744666949
1103	86-87	0.5675913570530469
1103	88-89	0.5604623385547747
1103	90-91	0.5463491523539936
1103	92-93	0.5999564010375629
1103	94-95	0.6031580909398997
1103	96-97	0.6420068781330848
1103	98-99	0.68567316456895
1103	100	0.6520623144500135
1104	1	0.26528993689052527
1104	2	0.2560873122509548
1104	3	0.18124998997665642
1104	4	0.16767347486742068
1104	5	0.1878794864777973
1104	6	0.18419307976567723
1104	7	0.22478329942770614
1104	8	0.22016391872543295
1104	9	0.19134839316477326
1104	10-11	0.2650088360591454
1104	12-13	0.20466416330496173
1104	14-15	0.3741744937193445
1104	16-17	0.34526422368170273
1104	18-19	0.3505603230954506
1104	20-21	0.331329405945759
1104	22-23	0.33328949993441626
1104	24-25	0.32557674575188145
1104	26-27	0.3923298950613443
1104	28-29	0.3537054706344165
1104	30-31	0.36422713698040354
1104	32-33	0.35490541607344994
1104	34-35	0.3792050356089831
1104	36-37	0.38721811656513694
1104	38-39	0.30225551148839
1104	40-41	0.28352275577027086
1104	42-43	0.21650790222096106
1104	44-45	0.26251130199032957
1104	46-47	0.2699934708091334
1104	48-49	0.28555320857953603
1104	50-51	0.2735184671476176
1104	52-53	0.26623576844955466
1104	54-55	0.33201692582130704
1104	56-57	0.3208954574858538
1104	58-59	0.23156658068947422
1104	60-61	0.24579275290396652
1104	62-63	0.22074607911979882
1104	64-65	0.22069460559471565
1104	66-67	0.18703583617292452
1104	68-69	0.24363589526277707
1104	70-71	0.24884409797062546
1104	72-73	0.24112193669347448
1104	74-75	0.23144327006399124
1104	76-77	0.16256362273742297
1104	78-79	0.2790733254363573
1104	80-81	0.2776862344406723
1104	82-83	0.29018037169788613
1104	84-85	0.31622545774670385
1104	86-87	0.2949833423350938
1104	88-89	0.315163246061136
1104	90-91	0.29205455772432387
1104	92-93	0.32735216492399033
1104	94-95	0.3518859281832718
1104	96-97	0.4133470732313107
1104	98-99	0.41891744026182565
1104	100	0.45915802764292124
1105	1	0.2182930080928287
1105	2	0.2084747776886502
1105	3	0.20696004348112496
1105	4	0.1518625245939873
1105	5	0.1667049236818272
1105	6	0.17924385322250913
1105	7	0.1735280041006959
1105	8	0.17859163478416207
1105	9	0.2538461532131038
1105	10-11	0.21406390355920024
1105	12-13	0.2222674812534322
1105	14-15	0.32074003525371353
1105	16-17	0.28616850832219853
1105	18-19	0.2782268082904835
1105	20-21	0.3191770230800941
1105	22-23	0.25622581671677835
1105	24-25	0.1701098374264305
1105	26-27	0.23486899293518348
1105	28-29	0.23044734035143222
1105	30-31	0.2292666052335477
1105	32-33	0.2728993734660605
1105	34-35	0.2292469252448015
1105	36-37	0.2658452433814702
1105	38-39	0.30389919919690556
1105	40-41	0.3320808038993377
1105	42-43	0.3001785184354233
1105	44-45	0.26782615366022355
1105	46-47	0.2662340903385143
1105	48-49	0.22222163795103
1105	50-51	0.2552254675725649
1105	52-53	0.26030128824471177
1105	54-55	0.2587160363339507
1105	56-57	0.2364091128407324
1105	58-59	0.2089033084282903
1105	60-61	0.25521131793221485
1105	62-63	0.26813630482809714
1105	64-65	0.2651808380898757
1105	66-67	0.20858252554264567
1105	68-69	0.2190354762282638
1105	70-71	0.20716389000884305
1105	72-73	0.2448447871586481
1105	74-75	0.2517460700828167
1105	76-77	0.1436437211335715
1105	78-79	0.25922314803218427
1105	80-81	0.29475415822712847
1105	82-83	0.27062275940956937
1105	84-85	0.2544218897038135
1105	86-87	0.22502285569203906
1105	88-89	0.2591687209296687
1105	90-91	0.31479840729450004
1105	92-93	0.2979646898153909
1105	94-95	0.3002309970348165
1105	96-97	0.24753806932857003
1105	98-99	0.31764830091607266
1105	100	0.3578719845349134
1106	1	0.17352285309107884
1106	2	0.1325230658182761
1106	3	0.1455718417478522
1106	4	0.10394113786689019
1106	5	0.1466166052840947
1106	6	0.13238531915541785
1106	7	0.13364019786396852
1106	8	0.15874026345115766
1106	9	0.1577843492964277
1106	10-11	0.16955556734987454
1106	12-13	0.16251404505441513
1106	14-15	0.217853859751294
1106	16-17	0.21623192239396616
1106	18-19	0.21790162096431942
1106	20-21	0.22522338114374207
1106	22-23	0.21680106755356832
1106	24-25	0.22150423307840583
1106	26-27	0.24590798391594149
1106	28-29	0.255578983208558
1106	30-31	0.23323551833605904
1106	32-33	0.2538786854889352
1106	34-35	0.19113852377923735
1106	36-37	0.28267291435929565
1106	38-39	0.23062890592626673
1106	40-41	0.27609717011826973
1106	42-43	0.24371206955003544
1106	44-45	0.21982750158593234
1106	46-47	0.20034537498354155
1106	48-49	0.3192548700879101
1106	50-51	0.359068165677499
1106	52-53	0.3486453913223144
1106	54-55	0.2523282201808854
1106	56-57	0.16546078633723482
1106	58-59	0.2994779040648865
1106	60-61	0.3213084755354316
1106	62-63	0.30886173710407405
1106	64-65	0.35396779229511566
1106	66-67	0.2866292729937783
1106	68-69	0.30527615775988437
1106	70-71	0.31428828754131644
1106	72-73	0.328451193150201
1106	74-75	0.3447758498895155
1106	76-77	0.2819283373590835
1106	78-79	0.35353652796909074
1106	80-81	0.3862841815627647
1106	82-83	0.34826199125416224
1106	84-85	0.3570006212884991
1106	86-87	0.4051386571622473
1106	88-89	0.381850776066468
1106	90-91	0.3396744648437213
1106	92-93	0.3849970363246662
1106	94-95	0.41299832798191005
1106	96-97	0.3997945994386285
1106	98-99	0.3881544854907659
1106	100	0.44400873733141566
1107	1	0.11729784591351944
1107	2	0.1144716945049069
1107	3	0.1460130401341857
1107	4	0.0833486089455917
1107	5	0.062109860731752065
1107	6	0.08922921554407282
1107	7	0.107132671413396
1107	8	0.062006924299979005
1107	9	0.07801808073375582
1107	10-11	0.11695587898838511
1107	12-13	0.1079594832377282
1107	14-15	0.2088911542690255
1107	16-17	0.1811934598422198
1107	18-19	0.15241905133506606
1107	20-21	0.1580496362937467
1107	22-23	0.13458653626825168
1107	24-25	0.13482487597053705
1107	26-27	0.16809662368764577
1107	28-29	0.2069403839118138
1107	30-31	0.20267401956184727
1107	32-33	0.23421755858956317
1107	34-35	0.2154649155277255
1107	36-37	0.12863666182221323
1107	38-39	0.19109762119604312
1107	40-41	0.2529052121040678
1107	42-43	0.2353402057668177
1107	44-45	0.22724200161019326
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2312	64-65	-0.16302335708734716
2312	66-67	-0.14717973160942677
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2312	98-99	-0.20989468418992985
2312	100	-0.22649786380287296
2313	1	-0.2656741482662497
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2313	98-99	-0.5724707990623266
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2314	1	-0.3835131137035148
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2314	4	-0.23514585058126158
2314	5	-0.23989180741317995
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2314	82-83	-0.5663169310649643
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2314	86-87	-0.4809939467309192
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2314	90-91	-0.49392757782296925
2314	92-93	-0.5015629224531075
2314	94-95	-0.5147360982673987
2314	96-97	-0.5507733681032612
2314	98-99	-0.6535493768788179
2314	100	-0.7323404314628768
2315	1	-0.33620020929254224
2315	2	-0.3198186790548405
2315	3	-0.31638577217294284
2315	4	-0.2625940152065951
2315	5	-0.2635733490720611
2315	6	-0.2733638358114945
2315	7	-0.2549108898522192
2315	8	-0.2746356902016984
2315	9	-0.36474041125750034
2315	10-11	-0.3350447475519758
2315	12-13	-0.36399507465198866
2315	14-15	-0.5780041922878993
2315	16-17	-0.5170226271181733
2315	18-19	-0.43107315011300784
2315	20-21	-0.4501311465051998
2315	22-23	-0.44739049467541747
2315	24-25	-0.48251294173050496
2315	26-27	-0.4424880821167463
2315	28-29	-0.4987592717898863
2315	30-31	-0.4078703257444687
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2315	66-67	-0.47835778769927373
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2315	96-97	-0.6239826186837654
2315	98-99	-0.609416787645852
2315	100	-0.6446788579277367
2316	1	-0.45232923427316507
2316	2	-0.42969315221101567
2316	3	-0.45543072508876037
2316	4	-0.33360004803281385
2316	5	-0.3731971666593523
2316	6	-0.3500812272312359
2316	7	-0.3939267270163427
2316	8	-0.3600581074726179
2316	9	-0.42608034628451463
2316	10-11	-0.40985620514436505
2316	12-13	-0.43091155199107334
2316	14-15	-0.9407045190106942
2316	16-17	-0.7715071429526787
2316	18-19	-0.681665079714989
2316	20-21	-0.6743102189946626
2316	22-23	-0.6099372404527088
2316	24-25	-0.589116159326295
2316	26-27	-0.6199152927374953
2316	28-29	-0.6565044001433762
2316	30-31	-0.7215186196728922
2316	32-33	-0.7709884445264592
2316	34-35	-0.7968017310684488
2316	36-37	-0.7699586933913523
2316	38-39	-0.6696034510440896
2316	40-41	-0.6780366549272685
2316	42-43	-0.6897628340611917
2316	44-45	-0.7875284889940914
2316	46-47	-0.7748582330636964
2316	48-49	-0.7551596563713403
2316	50-51	-0.7406010672373355
2316	52-53	-0.8066588070620284
2316	54-55	-0.979039750146903
2316	56-57	-0.995273480755003
2316	58-59	-1.0049654154515437
2316	60-61	-0.9147751214253219
2316	62-63	-0.9508230751320141
2316	64-65	-0.8010022142014179
2316	66-67	-0.7759460373568885
2316	68-69	-0.7740888502901626
2316	70-71	-0.8183628362882516
2316	72-73	-0.8080886528396078
2316	74-75	-0.8080063344284198
2316	76-77	-0.8148362127560169
2316	78-79	-0.8895051822431768
2316	80-81	-0.913529528593017
2316	82-83	-0.89875348744145
2316	84-85	-0.8909402249555107
2316	86-87	-0.8431251974001555
2316	88-89	-0.8325225733198884
2316	90-91	-0.9312716457103214
2316	92-93	-0.8821849778123543
2316	94-95	-0.8907670571495103
2316	96-97	-0.8733062945407788
2316	98-99	-0.8836150959065527
2316	100	-0.9850579333367371
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10.0
3	33.0
4	40.0
5	85.0
6	220.0
7	624.0
8	1404.0
9	2564.0
10	4775.0
11	8711.0
12	14836.0
13	21309.0
14	23324.0
15	28564.0
16	37104.0
17	49096.0
18	49653.0
19	32653.0
20	22846.0
21	20250.0
22	21603.0
23	24019.0
24	26527.0
25	28591.0
26	34217.0
27	42768.0
28	53950.0
29	68523.0
30	86743.0
31	109313.0
32	140488.0
33	186499.0
34	273866.0
35	464167.0
36	929190.0
37	1893894.0
38	1845973.0
39	350619.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33105313740754	18.377828319946424	13.61949574764479	23.671622795001255
2	15.367929555662698	22.38385493512755	35.924973445616985	26.32324206359276
3	19.503842872560647	27.017642166572504	29.61220802328827	23.866306937578578
4	14.042029121835812	18.51103848922777	34.09638489604614	33.35054749289028
5	14.120624355910119	36.85665295023966	32.67283350267492	16.349889191175297
6	31.684508662326454	33.6303995776999	17.897880078424677	16.787211681548968
7	26.0334909373465	32.48113339224702	21.903440594988606	19.581935075417874
8	27.990527815699977	31.114296270131764	21.174807688656294	19.720368225511965
9	28.200657661589386	14.773107911201915	19.630852030780545	37.395382396428154
10-11	25.24254789466674	25.77806141121226	25.841270181766163	23.138120512354842
12-13	27.250270641084196	23.832235719289287	26.178772549739765	22.738721089886756
14-15	25.95992613226823	23.5059735259464	24.81405881328976	25.720041528495617
16-17	23.68160578395761	26.43297007736516	27.007008911987327	22.8784152266899
18-19	25.02517987540582	25.463452474353055	28.10015394183721	21.411213708403924
20-21	24.874572720355705	25.2770546183814	27.37165340943556	22.476719251827337
22-23	23.606133405090084	26.235345385056853	25.261389506328563	24.8971317035245
24-25	23.75406465235971	26.973869398302448	24.818056347525204	24.454009601812636
26-27	24.49960181515468	25.306444332541023	25.418565183598435	24.77538866870586
28-29	24.014354840953995	24.568924282692787	27.79789382322656	23.618827053126655
30-31	25.597201725997532	24.787044205313634	26.755520065540246	22.860234003148587
32-33	22.407595969668883	25.85452484605066	27.467200836040778	24.270678348239684
34-35	22.38749623400844	26.38201653057398	27.505838419339508	23.724648816078066
36-37	24.26023924831278	26.269512863441374	26.10369311120062	23.366554777045227
38-39	24.75776552269316	24.775723714023556	26.14490173253064	24.32160903075264
40-41	23.615476755414182	25.917925532793813	26.23647705139061	24.230120660401397
42-43	25.55825715898739	25.081832010836045	26.109922969273953	23.249987860902614
44-45	25.10516837158066	24.68403830892824	26.736189471929617	23.474603847561486
46-47	23.678627506428633	25.13654101506419	28.066188457256473	23.118643021250698
48-49	24.377573273524245	25.87504707008812	27.250754643306568	22.496625013081065
50-51	23.12283454158376	26.575059368926862	26.085381677264944	24.216724412224433
52-53	22.760589830869133	26.890526389943613	24.731372245758322	25.61751153342893
54-55	23.56549096116606	26.092820533633276	24.71076021289672	25.630928292303945
56-57	24.020078834740932	24.655944364774584	25.098905891408602	26.225070909075882
58-59	23.711439516019137	24.583908610514975	26.72509946182469	24.979552411641194
60-61	24.020556091909913	25.240672659161152	25.930969055611293	24.807802193317638
62-63	21.8323127410564	25.877595320110135	26.53906436414168	25.751027574691786
64-65	21.850216228846012	25.763135054467405	26.08614483013324	26.300503886553344
66-67	23.493777784799494	25.787301512298978	24.98225526873937	25.736665434162155
68-69	23.94547434703345	24.82095435546322	25.041119767955223	26.192451529548112
70-71	22.84760801691867	25.378466318392768	25.36158069177151	26.41234497291705
72-73	23.968682625534786	24.84131613736051	25.418312787756385	25.771688449348318
74-75	24.11965848263479	24.34061669001576	25.7511720264257	25.788552800923746
76-77	22.731710590643377	25.372243907975108	26.647292257033932	25.248753244347583
78-79	22.925396922717457	25.22846691449469	26.468730373456744	25.377405789331103
80-81	22.288499229335628	25.464717718265284	25.98713742756088	26.259645624838207
82-83	22.198383116801704	25.95385027803338	24.78716019098566	27.060606414179254
84-85	22.374269880619675	25.422178119487004	24.86212218129384	27.341429818599476
86-87	22.641566863819218	24.246727263543217	25.448289361668824	27.66341651096874
88-89	22.585271393671384	23.911508349766173	26.410299892960076	27.092920363602367
90-91	22.964181066170074	24.271376624219922	25.907046963523317	26.857395346086683
92-93	21.65472755178017	24.730911535949023	26.259209071746444	27.355151840524368
94-95	21.11499659785386	24.901919703263577	26.63285802814088	27.35022567074168
96-97	22.189148274668305	24.57942261120225	25.813458345994544	27.417970768134904
98-99	22.38726090351292	24.097680668595444	25.786221589570857	27.728836838320774
100	22.185196775433063	24.77704891277328	25.973977407971027	27.06377690382262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	928.0
1	641.5
2	404.5
3	483.5
4	431.5
5	505.0
6	861.0
7	1574.5
8	2528.0
9	2738.5
10	2127.0
11	1824.0
12	2030.0
13	2217.0
14	2461.5
15	2823.5
16	3134.5
17	3541.0
18	4048.5
19	4516.0
20	5172.0
21	6072.5
22	7355.5
23	8970.5
24	10703.0
25	13025.5
26	16558.5
27	21045.0
28	25693.5
29	32123.5
30	40461.5
31	48481.0
32	56914.5
33	66327.5
34	75951.5
35	84117.5
36	92326.5
37	102622.5
38	110172.0
39	115013.0
40	119305.5
41	123770.0
42	131384.5
43	146414.0
44	165771.5
45	182615.0
46	209455.5
47	249949.5
48	352632.0
49	464778.5
50	580779.5
51	573070.5
52	400836.5
53	298820.0
54	258402.0
55	228010.5
56	205820.5
57	186014.5
58	176487.0
59	162995.0
60	137782.0
61	114535.5
62	95779.0
63	74825.5
64	55244.5
65	45268.5
66	35762.5
67	26121.0
68	20337.0
69	16690.5
70	14347.0
71	12707.5
72	11114.5
73	11543.0
74	9257.5
75	6850.0
76	5237.5
77	3385.0
78	2372.5
79	1426.5
80	915.5
81	648.0
82	485.5
83	360.0
84	232.5
85	147.0
86	97.0
87	44.5
88	26.0
89	17.5
90	8.5
91	4.0
92	3.0
93	2.5
94	1.5
95	1.5
96	2.5
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012015608986284088
2	0.045424509726193406
3	0.0013479513096796383
4	0.0
5	0.00128997490926331
6	0.0
7	0.0
8	0.0
9	9.566106068694207E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.159992544234906E-4
22-23	0.0018045154629582254
24-25	0.0035510545255001227
26-27	0.00801523735755742
28-29	0.003232184323210316
30-31	0.005681687240800196
32-33	0.002521973418110291
34-35	0.0064571215963685905
36-37	0.0
38-39	7.247050052041066E-6
40-41	0.0023335501167572235
42-43	0.0023770324170694695
44-45	0.0026669144191511125
46-47	0.00217411501561232
48-49	0.0010580693075979957
50-51	0.0018407507132184309
52-53	0.007247050052041067
54-55	0.0034640899248756297
56-57	0.03110433882336026
58-59	0.017900213628541436
60-61	0.0210526804011793
62-63	0.015610145812096457
64-65	0.002710396719463359
66-67	0.0018552448133225129
68-69	1.4494100104082132E-5
70-71	0.0
72-73	0.0026886555693072358
74-75	0.001311716059419433
76-77	7.536932054122709E-4
78-79	0.003188702022898069
80-81	0.0016958097121776096
82-83	0.0014131747601480078
84-85	0.004580135632889954
86-87	7.029638550479835E-4
88-89	9.276224066612564E-4
90-91	6.812227048918603E-4
92-93	6.957168049959424E-4
94-95	5.4352875390308E-4
96-97	6.522345046836959E-5
98-99	0.0
100	0.0028988200208164266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6899359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.849749878610226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7041995382038	31.36342665993651
2	11.832138918632104	9.43015552872918
3	3.5717799896548614	4.270036176275138
4	1.6941657647186348	2.7004832790776803
5	0.9457488440346696	1.884392744138317
6	0.6098096105609041	1.458045627265484
7	0.41805094385888186	1.1661457884505355
8	0.3078224508016428	0.9813318137173013
9	0.23613784742396293	0.8469030741046512
>10	1.4358238498523632	10.68484365477061
>50	0.11995570990791006	3.3427471411343954
>100	0.09923161269894325	8.144901256081987
>500	0.014300287299924518	3.897627619521719
>1k	0.008974139927527226	6.6362564453675565
>5k	7.660851157557807E-4	2.118838484682417
>10k+	0.001094407308222544	11.073864706746662
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	91760	1.3299786255505766	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	76020	1.1018414899123237	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	51054	0.7399817867138092	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	40634	0.5889532636292734	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	40389	0.5854022091037733	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	37779	0.5475726078321189	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	37724	0.5467754323263944	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	37361	0.5415140739886126	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	33743	0.4890744198120434	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	29706	0.4305617376918638	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	27076	0.39244225441812786	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	26172	0.3793395879240376	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	17572	0.25469032702893124	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	16679	0.2417470956359859	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	16323	0.23658719599893266	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16319	0.2365292195985163	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	14759	0.21391842343614823	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	14183	0.2055698217761969	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	13753	0.19933735873144154	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	12926	0.18735073794536566	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12026	0.17430604785169174	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	11580	0.1678416792052711	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	11422	0.16555161138882613	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	11274	0.16340648457342197	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	10835	0.15704357462772991	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	10772	0.15613044632117273	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	10756	0.15589854071950743	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	10610	0.15378240210431143	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	10446	0.15140536968724197	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	10103	0.14643389335154178	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9492	0.1375779981879476	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8435	0.1222577343779328	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	8242	0.11946037305784493	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	8104	0.11746018724348159	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	8025	0.11631515333525912	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	8018	0.11621369463453053	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	7791	0.1129235339109039	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	7591	0.11002471389008747	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7189	0.10419808564824645	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	7068	0.10244429953565252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003927901128206258	0.0	0.0	0.0	0.0
2	0.005855616442049182	0.0	0.0	0.0	0.0
3	0.006159992544234907	0.0	0.0	0.0	0.0
4	0.007493449753810463	0.0	0.0	2.8988200208164267E-5	0.0
5	0.02527771058151924	1.4494100104082134E-5	0.0	2.6089380187347837E-4	0.0
6	0.0591359284246551	1.4494100104082134E-5	0.0	5.507758039551211E-4	0.0
7	0.06812227048918602	1.4494100104082134E-5	0.0	6.52234504683696E-4	0.0
8	0.07948564497078642	1.4494100104082134E-5	0.0	7.391991053081888E-4	1.4494100104082134E-5
9	0.08763132922928057	1.4494100104082134E-5	0.0	8.406578060367637E-4	1.4494100104082134E-5
10-11	0.1643703422303434	1.4494100104082134E-5	0.0	0.0016088451115531168	1.4494100104082134E-5
12-13	0.2794027677063913	1.4494100104082134E-5	0.0	0.002840843620400098	1.4494100104082134E-5
14-15	0.40672619007070077	2.17411501561232E-5	0.0	0.00428300658075627	5.7976400416328535E-5
16-17	0.45796283393863113	2.8988200208164267E-5	0.0	0.005000464535908336	5.7976400416328535E-5
18-19	0.48185635796021054	2.8988200208164267E-5	0.0	0.005500510989499169	7.971755057245173E-5
20-21	0.5592476054659571	2.8988200208164267E-5	0.0	0.007652884854955366	8.69646006244928E-5
22-23	0.582162777730511	2.8988200208164267E-5	0.0	0.011617021233421829	8.69646006244928E-5
24-25	0.6801573885342103	2.8988200208164267E-5	0.0	0.0233644893677804	8.69646006244928E-5
26-27	0.8949599520767074	2.8988200208164267E-5	0.0	0.054048499288122276	9.421165067653386E-5
28-29	0.9495664742188368	2.8988200208164267E-5	0.0	0.11371346236657637	1.1595280083265707E-4
30-31	0.9686262158557049	2.8988200208164267E-5	0.0	0.24720412432517283	1.1595280083265707E-4
32-33	0.985243701625035	2.8988200208164267E-5	0.0	0.45747003453509233	1.1595280083265707E-4
34-35	0.9978463216655344	2.8988200208164267E-5	0.0	0.7424530307815552	1.1595280083265707E-4
36-37	1.0189062491167657	3.6235250260205335E-5	0.0	1.1263872484385868	1.2319985088469812E-4
38-39	1.0430606669402187	5.0729350364287463E-5	0.0	1.6650677258568511	1.3044690093673918E-4
40-41	1.0656569690024826	5.7976400416328535E-5	0.0	2.402592762603019	1.3044690093673918E-4
42-43	1.1123497124877832	5.7976400416328535E-5	0.0	2.9361568226845423	1.3044690093673918E-4
44-45	1.1639197206581073	5.7976400416328535E-5	0.0	3.4984699303225124	1.3769395098878028E-4
46-47	1.1820590869383663	5.7976400416328535E-5	0.0	4.119360943531131	1.5943510114490347E-4
48-49	1.192951403166584	5.7976400416328535E-5	0.0	4.664136190043163	1.739292012489856E-4
50-51	1.214815753173592	5.7976400416328535E-5	0.0	5.239950551928085	1.739292012489856E-4
52-53	1.222236732426882	5.7976400416328535E-5	0.0	5.933681665209768	1.956703514051088E-4
54-55	1.246572326501636	5.7976400416328535E-5	0.0	6.546680640911713	2.1741150156123198E-4
56-57	1.292127283128766	5.7976400416328535E-5	0.0	7.175398468176537	2.1741150156123198E-4
58-59	1.3073678293882085	5.7976400416328535E-5	0.0	7.8191901595496045	2.2465855161327305E-4
60-61	1.3195718616758456	5.7976400416328535E-5	0.0	8.383242559200065	2.3190560166531414E-4
62-63	1.3296959905985468	5.7976400416328535E-5	0.0	8.944323668329188	2.3190560166531414E-4
64-65	1.33734162840345	5.7976400416328535E-5	0.0	9.558467677939355	2.3190560166531414E-4
66-67	1.3484513561332292	5.7976400416328535E-5	0.0	10.197229916576308	2.3190560166531414E-4
68-69	1.3617424459286724	6.52234504683696E-5	0.0	10.945306658198247	2.3190560166531414E-4
70-71	1.378077296745973	7.247050052041067E-5	0.0	11.811343633517257	2.3190560166531414E-4
72-73	1.4158271804670548	7.247050052041067E-5	0.0	12.513916147862432	2.4639970176939624E-4
74-75	1.452200124678249	7.247050052041067E-5	0.0	13.194914194202678	2.4639970176939624E-4
76-77	1.463483781609277	7.247050052041067E-5	0.0	13.913915771015828	2.6089380187347837E-4
78-79	1.4718758655695405	7.247050052041067E-5	0.0	14.604842275927373	2.6089380187347837E-4
80-81	1.4877034228831982	7.247050052041067E-5	0.0	15.363593922275967	2.6089380187347837E-4
82-83	1.4943199795807118	7.247050052041067E-5	0.0	16.23087739020393	2.6089380187347837E-4
84-85	1.5152204719307982	7.247050052041067E-5	0.0	17.029792187940938	2.6089380187347837E-4
86-87	1.551165840188922	8.69646006244928E-5	0.0	17.811712653305907	2.6089380187347837E-4
88	1.5603333585047539	8.69646006244928E-5	0.0	18.428596047835747	2.6089380187347837E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	2430	0.0	29.009735	3
CGATCTC	2635	0.0	24.4337	5
CCGATCT	3205	0.0	22.874199	4
GATCTAC	2825	0.0	21.97278	1
AGTACTC	3020	0.0	20.696362	5
TCTACAC	3800	0.0	19.416672	3
TCTCGCG	1120	0.0	18.881681	8
ATCTACA	3475	0.0	18.536963	2
TACACTC	3620	0.0	18.434404	5
TTAACGC	405	3.274181E-11	17.405462	4
CTAACGC	600	0.0	17.231781	3
TCTAACG	620	0.0	16.68414	2
AGTACTT	23590	0.0	16.504816	12-13
CTCGCGC	1385	0.0	15.268941	9
GAGTACT	22925	0.0	15.128407	12-13
ATCTCGC	2870	0.0	15.064409	7
AAAGTAC	4185	0.0	15.047629	3
GTAATAC	1095	0.0	15.021478	3
AAGTACT	4270	0.0	14.857824	4
CTACACT	5330	0.0	14.636263	4
>>END_MODULE