FastQCFastQC Report
Fri 27 May 2016
ERR765247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8817010
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT749960.8505831341917498No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA743770.8435626136297905No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG284710.322909920710082No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC274780.3116475993562443No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG269400.3055457575754139No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA267460.30334546518604383No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC245790.2787679723625129No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG234550.2660198865601831No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC213530.24217960510422468No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT184500.20925461125710418No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT174120.19748191280263944No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC149590.1696606899617898No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG123690.1402856523923643No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA106200.12044899574799167No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC97680.1107858559761189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.10673686431114404No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC91250.10349313429382523No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC90090.10217749554554208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACA80650.030.2198752
TACACTC90800.028.6529625
TCTACAC96500.027.9825733
CTACACT121400.024.3769594
TAGTACT24600.022.3768164
CACTCTT115350.022.0477397
GTATAAG23900.021.0804331
CTAGTAC28350.020.9083863
ACACTCT127750.020.2388676
CATGGGG156600.020.219524
ACTCTTT146350.020.0436258
CTGTGCG35150.018.7235039
GTACTAG22000.018.4064241
CGTATCA55100.018.28995794
CCTAGTA31200.018.2472762
CATGGGT78150.018.1813224
AGTACTT179350.018.09512312-13
CGCCGTA100450.017.0179594
GAGTACT171150.016.82009312-13
TGGTCGC278450.016.69246594
Produced by FastQC (version 0.11.5)