##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765247_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8817010 Sequences flagged as poor quality 0 Sequence length 100 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24876811980479 33.0 31.0 34.0 31.0 34.0 2 32.294563236289854 34.0 31.0 34.0 31.0 34.0 3 32.33435291555754 34.0 31.0 34.0 30.0 34.0 4 35.790573675202815 37.0 35.0 37.0 35.0 37.0 5 35.79812328669243 37.0 35.0 37.0 35.0 37.0 6 35.88421244843774 37.0 37.0 37.0 35.0 37.0 7 35.82648891177394 37.0 37.0 37.0 35.0 37.0 8 35.85086713069396 37.0 37.0 37.0 35.0 37.0 9 37.56937782763091 39.0 38.0 39.0 35.0 39.0 10-11 37.48460759373076 39.0 38.0 39.0 35.0 39.0 12-13 37.4631784471153 39.0 38.0 39.0 35.0 39.0 14-15 38.88110464885489 41.0 39.0 41.0 35.5 41.0 16-17 38.82529888250099 41.0 39.0 41.0 35.0 41.0 18-19 38.77442647791031 41.0 38.5 41.0 35.0 41.0 20-21 38.741998421233504 40.5 39.0 41.0 35.0 41.0 22-23 38.655046608771 40.0 38.5 41.0 34.5 41.0 24-25 38.58270575852812 40.0 38.0 41.0 34.0 41.0 26-27 38.47218303030165 40.0 38.0 41.0 34.0 41.0 28-29 38.342676825817364 40.0 38.0 41.0 34.0 41.0 30-31 38.167920814425756 40.0 38.0 41.0 34.0 41.0 32-33 37.971270702880005 40.0 38.0 41.0 33.0 41.0 34-35 37.821858600591355 40.0 38.0 41.0 33.0 41.0 36-37 37.68616038770513 40.0 37.5 41.0 33.0 41.0 38-39 37.51898682206326 40.0 37.0 41.0 33.0 41.0 40-41 37.30773164598883 40.0 37.0 41.0 32.0 41.0 42-43 37.096165196591585 40.0 36.5 41.0 31.0 41.0 44-45 36.83211213325152 40.0 36.0 41.0 31.0 41.0 46-47 36.502878073179005 39.0 35.5 41.0 30.5 41.0 48-49 36.1975617584646 39.0 35.0 41.0 30.0 41.0 50-51 34.91964747686574 38.0 34.0 40.0 27.5 40.5 52-53 34.88850562719108 38.0 34.0 39.5 28.0 40.0 54-55 35.794546507262666 39.0 35.0 40.5 29.5 41.0 56-57 35.83658904776109 39.0 35.0 41.0 30.0 41.0 58-59 35.62485604530334 38.0 35.0 41.0 29.5 41.0 60-61 35.29246581324054 37.5 35.0 40.0 29.0 41.0 62-63 34.927147695193725 37.0 35.0 40.0 28.0 41.0 64-65 34.58518789249417 36.5 34.5 39.5 28.0 41.0 66-67 34.25112566504971 36.0 34.0 39.0 28.0 41.0 68-69 33.97994802092773 35.0 34.0 39.0 28.0 41.0 70-71 33.60401904954173 35.0 34.0 37.5 27.0 40.5 72-73 33.2192895323925 35.0 34.0 37.0 26.0 39.0 74-75 32.86692183631413 35.0 34.0 36.5 26.0 39.0 76-77 32.526624728791276 35.0 34.0 36.0 26.0 38.5 78-79 32.1972840566133 35.0 33.5 35.5 25.0 37.0 80-81 31.90064727158073 35.0 33.0 35.0 24.5 37.0 82-83 31.664124175882755 35.0 33.0 35.0 24.0 36.0 84-85 31.423074999347854 35.0 33.0 35.0 23.5 36.0 86-87 31.184825751586988 35.0 33.0 35.0 21.5 36.0 88-89 30.958281719086173 35.0 33.0 35.0 19.5 35.5 90-91 30.687252027614804 35.0 32.5 35.0 17.5 35.0 92-93 30.37434090468311 35.0 32.0 35.0 9.5 35.0 94-95 30.000292729621492 35.0 32.0 35.0 2.0 35.0 96-97 29.63263322827126 35.0 31.0 35.0 2.0 35.0 98-99 29.271518746150907 35.0 31.0 35.0 2.0 35.0 100 28.655820056912717 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46781500701448664 1101 2 0.5339081225864106 1101 3 0.5756040935840332 1101 4 0.4847404641350934 1101 5 0.5447992700310706 1101 6 0.4695886279243666 1101 7 0.48152785313847346 1101 8 0.45135828361211594 1101 9 0.5291005296250546 1101 10-11 0.5360920409385557 1101 12-13 0.5959229591757449 1101 14-15 0.7181257117737658 1101 16-17 0.7672933805297504 1101 18-19 0.6816120723491892 1101 20-21 0.6851811355556521 1101 22-23 0.7255657966292901 1101 24-25 0.666690108422948 1101 26-27 0.7663994466342032 1101 28-29 0.7681481772073937 1101 30-31 0.7233018923964281 1101 32-33 0.6761195713407275 1101 34-35 0.7957976155052862 1101 36-37 0.7295741579361632 1101 38-39 0.7622144740848142 1101 40-41 0.7663330567271913 1101 42-43 0.7641733950180551 1101 44-45 0.7886810379500773 1101 46-47 0.8704271338534255 1101 48-49 0.7817691113308101 1101 50-51 0.7397006186210504 1101 52-53 0.7827576995397436 1101 54-55 0.8701288610084958 1101 56-57 0.9193092608237947 1101 58-59 0.9409473872882259 1101 60-61 0.9486494578269955 1101 62-63 0.883957251413257 1101 64-65 0.8353935443696088 1101 66-67 0.8284628222860135 1101 68-69 0.8258094656423651 1101 70-71 0.7522859489590346 1101 72-73 0.7659794264127129 1101 74-75 0.7330432557027748 1101 76-77 0.7066195543408966 1101 78-79 0.6916348621410862 1101 80-81 0.671101551916685 1101 82-83 0.7154338548956609 1101 84-85 0.684944662789448 1101 86-87 0.6788884349209319 1101 88-89 0.6317651464589318 1101 90-91 0.6235460489968823 1101 92-93 0.60610909138315 1101 94-95 0.5857873101388442 1101 96-97 0.3430432927938938 1101 98-99 0.24590678382291387 1101 100 0.33923230495123846 1102 1 0.4441876204911779 1102 2 0.5570219018900033 1102 3 0.6084688735424422 1102 4 0.4950855937748031 1102 5 0.5040341017105021 1102 6 0.4450275723620294 1102 7 0.5060756206262553 1102 8 0.4776176344334857 1102 9 0.4560414396730579 1102 10-11 0.5412913141271503 1102 12-13 0.500658538061451 1102 14-15 0.6865865329437213 1102 16-17 0.6905489654050143 1102 18-19 0.6893828854570501 1102 20-21 0.6455555683746894 1102 22-23 0.7103193507760466 1102 24-25 0.7247103704227911 1102 26-27 0.7212699391424664 1102 28-29 0.6868583481045931 1102 30-31 0.7290433983583782 1102 32-33 0.750267159538673 1102 34-35 0.7623638613498755 1102 36-37 0.7423589714519281 1102 38-39 0.7872967394965826 1102 40-41 0.79887389854985 1102 42-43 0.8361011806133121 1102 44-45 0.837927699982437 1102 46-47 0.7810277449065239 1102 48-49 0.777718562437471 1102 50-51 0.6826857548188556 1102 52-53 0.7157172809852241 1102 54-55 0.7987227810863473 1102 56-57 0.8292570043137104 1102 58-59 0.8111669611509171 1102 60-61 0.7656973302641248 1102 62-63 0.7532289426248511 1102 64-65 0.7936519339541377 1102 66-67 0.7409703381465818 1102 68-69 0.6964960062442174 1102 70-71 0.6357516176318043 1102 72-73 0.6099369058963617 1102 74-75 0.6013166555227585 1102 76-77 0.5729498872798828 1102 78-79 0.5625624391598549 1102 80-81 0.5468861846220676 1102 82-83 0.5514616924201938 1102 84-85 0.49652105483921716 1102 86-87 0.5717794803122693 1102 88-89 0.56045720656995 1102 90-91 0.559738830531348 1102 92-93 0.5528444395455843 1102 94-95 0.509957623588658 1102 96-97 0.5030108527034436 1102 98-99 0.4863624590041944 1102 100 0.5428017726580769 1103 1 0.5223263497018067 1103 2 0.5824538342757748 1103 3 0.5773442302188414 1103 4 0.4793378204122547 1103 5 0.47045578973076374 1103 6 0.4814270671840788 1103 7 0.48409052023307453 1103 8 0.4935070153748171 1103 9 0.5361256401063201 1103 10-11 0.5581749367916373 1103 12-13 0.5361021817267471 1103 14-15 0.665479578243044 1103 16-17 0.6784962994636317 1103 18-19 0.6838637639663148 1103 20-21 0.6709096720520193 1103 22-23 0.7275031654857926 1103 24-25 0.682992857823379 1103 26-27 0.673909475448518 1103 28-29 0.6455752712886422 1103 30-31 0.6661134956504284 1103 32-33 0.681813975848442 1103 34-35 0.6731659068337592 1103 36-37 0.6392119001499239 1103 38-39 0.6440740147461526 1103 40-41 0.6991096338817115 1103 42-43 0.6749019653090969 1103 44-45 0.6872768011779371 1103 46-47 0.6337340487546044 1103 48-49 0.555406063843229 1103 50-51 0.553545651127358 1103 52-53 0.6095888710109492 1103 54-55 0.6232914803270688 1103 56-57 0.6159004456120343 1103 58-59 0.5960590716430332 1103 60-61 0.6290441372689486 1103 62-63 0.6261460926398357 1103 64-65 0.6010639630188166 1103 66-67 0.5786977275356833 1103 68-69 0.5495507214284174 1103 70-71 0.5668818993772717 1103 72-73 0.5268705040814652 1103 74-75 0.5888714260127941 1103 76-77 0.5661095880306632 1103 78-79 0.5884479718012372 1103 80-81 0.5464711289881024 1103 82-83 0.49103102790907016 1103 84-85 0.532923261033531 1103 86-87 0.5687457936686435 1103 88-89 0.5268542765624851 1103 90-91 0.5627039317810762 1103 92-93 0.5203068631611423 1103 94-95 0.408892054288291 1103 96-97 0.4908996765810194 1103 98-99 0.5319053173266788 1103 100 0.5577450079229251 1104 1 0.4556734380044887 1104 2 0.558695547165577 1104 3 0.5495889875316422 1104 4 0.48089391123692593 1104 5 0.4609473747726014 1104 6 0.46800749744044623 1104 7 0.47683066121403783 1104 8 0.4375455917174662 1104 9 0.5390199548519234 1104 10-11 0.5641189759623515 1104 12-13 0.5688773938531497 1104 14-15 0.686463412875419 1104 16-17 0.6617705232416071 1104 18-19 0.6756915597261468 1104 20-21 0.6007174153903492 1104 22-23 0.6425908387477861 1104 24-25 0.6671581923249121 1104 26-27 0.5787822816708683 1104 28-29 0.6559410135078636 1104 30-31 0.6664698709157406 1104 32-33 0.6858345338331304 1104 34-35 0.7076496475933496 1104 36-37 0.6269342766282264 1104 38-39 0.6833342424305897 1104 40-41 0.6655093249144954 1104 42-43 0.7453992318013363 1104 44-45 0.7369138916311115 1104 46-47 0.6847176843674845 1104 48-49 0.6928019965904397 1104 50-51 0.7130370324713979 1104 52-53 0.7165415996256783 1104 54-55 0.7824934589565942 1104 56-57 0.7559636325743995 1104 58-59 0.7575750353069068 1104 60-61 0.743552426066401 1104 62-63 0.7006554329044192 1104 64-65 0.6956140769699388 1104 66-67 0.6472918078447663 1104 68-69 0.6224418204178761 1104 70-71 0.612558780000235 1104 72-73 0.5935564035467848 1104 74-75 0.5228621161188229 1104 76-77 0.5036269974426588 1104 78-79 0.5258471486811729 1104 80-81 0.5871777758709271 1104 82-83 0.5758466729730323 1104 84-85 0.5714577865818029 1104 86-87 0.5659686856802821 1104 88-89 0.5633937851139592 1104 90-91 0.5322389467102191 1104 92-93 0.5313048469671671 1104 94-95 0.39722779617926207 1104 96-97 0.38604780191712607 1104 98-99 0.3154240724018642 1104 100 0.32077890550527144 1105 1 0.3534769589496918 1105 2 0.3517708400961297 1105 3 0.3755639595276534 1105 4 0.37539696278203394 1105 5 0.3991658102834279 1105 6 0.3619651936461281 1105 7 0.32155885589060773 1105 8 0.31564464358262256 1105 9 0.29605401080655014 1105 10-11 0.3795871758841187 1105 12-13 0.4098725576097735 1105 14-15 0.4803982724998903 1105 16-17 0.4838811956483511 1105 18-19 0.5046986296205347 1105 20-21 0.4754697412766973 1105 22-23 0.4965578473890204 1105 24-25 0.5187138858013185 1105 26-27 0.4050250695340267 1105 28-29 0.44742177410492445 1105 30-31 0.540549045509465 1105 32-33 0.5275727199307099 1105 34-35 0.46002720795748786 1105 36-37 0.49237152770393067 1105 38-39 0.5010330373703127 1105 40-41 0.5162717111205382 1105 42-43 0.5105562485770534 1105 44-45 0.5213000321044206 1105 46-47 0.3841586881991361 1105 48-49 0.49746157595284046 1105 50-51 0.37496672913936635 1105 52-53 0.4179921948214371 1105 54-55 0.46382799875780734 1105 56-57 0.45987013260995724 1105 58-59 0.5102943109372191 1105 60-61 0.4647762501213748 1105 62-63 0.4826228960547283 1105 64-65 0.4912127843416485 1105 66-67 0.38634608445299534 1105 68-69 0.3719899505963937 1105 70-71 0.3930556173282227 1105 72-73 0.34792646951172657 1105 74-75 0.377889414445697 1105 76-77 0.38859772105669066 1105 78-79 0.39967517644511474 1105 80-81 0.3560711573740747 1105 82-83 0.3471356729641393 1105 84-85 0.2882773731452275 1105 86-87 0.29169801162886344 1105 88-89 0.29459180549908126 1105 90-91 0.2997596329856016 1105 92-93 0.21967941579473305 1105 94-95 0.22023804096963318 1105 96-97 0.19617399208639696 1105 98-99 0.19831144416140134 1105 100 0.19887379011108308 1106 1 0.24536775549862 1106 2 0.2654177157044373 1106 3 0.35496675289984125 1106 4 0.3738071328522068 1106 5 0.3071943565769928 1106 6 0.322639224468368 1106 7 0.31932428293988835 1106 8 0.3090563776923787 1106 9 0.31074364427193046 1106 10-11 0.3503815273808826 1106 12-13 0.47072084652287316 1106 14-15 0.460962054836763 1106 16-17 0.47897009411546776 1106 18-19 0.47841616419747623 1106 20-21 0.4509614401397499 1106 22-23 0.4509297832761874 1106 24-25 0.5003306747189384 1106 26-27 0.4437748870418403 1106 28-29 0.5133984776287477 1106 30-31 0.5595230985694144 1106 32-33 0.5597378696545761 1106 34-35 0.5527799916218399 1106 36-37 0.5494561033638163 1106 38-39 0.5570132217288162 1106 40-41 0.5504122212006308 1106 42-43 0.5283792838949992 1106 44-45 0.5238349411041625 1106 46-47 0.51463734984533 1106 48-49 0.5421922848979932 1106 50-51 0.4762620599481622 1106 52-53 0.5236640300933288 1106 54-55 0.5121867747005524 1106 56-57 0.5854661013010727 1106 58-59 0.5541115006729029 1106 60-61 0.5390584961148335 1106 62-63 0.5091906779807047 1106 64-65 0.5030037237988907 1106 66-67 0.5533237247073401 1106 68-69 0.5491126122506103 1106 70-71 0.5474407948736726 1106 72-73 0.46395591233736155 1106 74-75 0.5625665158401105 1106 76-77 0.5148379859450429 1106 78-79 0.5053194105573837 1106 80-81 0.6041046237448455 1106 82-83 0.5418035475284135 1106 84-85 0.4942074301605537 1106 86-87 0.46878959160233435 1106 88-89 0.45226197570220705 1106 90-91 0.4445799230189955 1106 92-93 0.45243271244400063 1106 94-95 0.3404347101673615 1106 96-97 0.3371238485635004 1106 98-99 0.3473225795086492 1106 100 0.37092861703574087 1107 1 0.26792389285694185 1107 2 0.2944106467238683 1107 3 0.34599531878973977 1107 4 0.2927752887518551 1107 5 0.35535814216025585 1107 6 0.3416211748035778 1107 7 0.3232318935465486 1107 8 0.3371914382214172 1107 9 0.38055624634225893 1107 10-11 0.3754472679393075 1107 12-13 0.3340339340836067 1107 14-15 0.4046787329799173 1107 16-17 0.43642230134562965 1107 18-19 0.4383515875445525 1107 20-21 0.4365124002594598 1107 22-23 0.3724179491628732 1107 24-25 0.4296925473809097 1107 26-27 0.42054580649061535 1107 28-29 0.4555294633418967 1107 30-31 0.43267966399067603 1107 32-33 0.37618555981669743 1107 34-35 0.3913630845032472 1107 36-37 0.4649547206835578 1107 38-39 0.4966822040434593 1107 40-41 0.4775414679498482 1107 42-43 0.49221301415217766 1107 44-45 0.4556197663135393 1107 46-47 0.5486606995981589 1107 48-49 0.5205158113223618 1107 50-51 0.5650555382920217 1107 52-53 0.6004731425952841 1107 54-55 0.5936428223689205 1107 56-57 0.5529175035655385 1107 58-59 0.47679699134477715 1107 60-61 0.5416623482283782 1107 62-63 0.5007439375213565 1107 64-65 0.5507086237815457 1107 66-67 0.5842777036893452 1107 68-69 0.5776869494972061 1107 70-71 0.49927536191200517 1107 72-73 0.5422356692963604 1107 74-75 0.5177456529290723 1107 76-77 0.5435529672158737 1107 78-79 0.512754885648107 1107 80-81 0.5570647784074083 1107 82-83 0.59209388817521 1107 84-85 0.5442168891606123 1107 86-87 0.5788945584903153 1107 88-89 0.6045410054343563 1107 90-91 0.6269630151050087 1107 92-93 0.5768726364433938 1107 94-95 0.5862619587234938 1107 96-97 0.570651203884271 1107 98-99 0.5007913647939333 1107 100 0.565335336272792 1108 1 0.20870583751131022 1108 2 0.2883164726138503 1108 3 0.3142670457457868 1108 4 0.27589475707034694 1108 5 0.2495597943317378 1108 6 0.23665605006218016 1108 7 0.2991389852056372 1108 8 0.2653328852932404 1108 9 0.33454501926158287 1108 10-11 0.37766083406642537 1108 12-13 0.36902559427133497 1108 14-15 0.4458360490620237 1108 16-17 0.4135909381928684 1108 18-19 0.4542300474998271 1108 20-21 0.4448906881440067 1108 22-23 0.3804667980814074 1108 24-25 0.41733516098672396 1108 26-27 0.4090987162514139 1108 28-29 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2316 58-59 -1.211232165048763 2316 60-61 -1.1028522886932564 2316 62-63 -1.171117720944224 2316 64-65 -1.012745667850929 2316 66-67 -1.0708562635851564 2316 68-69 -0.9698815060219701 2316 70-71 -0.8598208277777744 2316 72-73 -0.8838918855652409 2316 74-75 -0.7649317114863265 2316 76-77 -0.733869599821837 2316 78-79 -0.6343677122176068 2316 80-81 -0.591074720955973 2316 82-83 -0.49002491727216224 2316 84-85 -0.492701186431713 2316 86-87 -0.5504955616104503 2316 88-89 -0.5070080802730814 2316 90-91 -0.3902541413670768 2316 92-93 -0.33732577372897765 2316 94-95 -0.15699464296476506 2316 96-97 -0.26237176922291283 2316 98-99 -0.015569260467852786 2316 100 -0.047597056232362434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24654.0 3 3732.0 4 3607.0 5 4225.0 6 5125.0 7 6092.0 8 7566.0 9 8869.0 10 10921.0 11 14781.0 12 20719.0 13 27708.0 14 27523.0 15 32688.0 16 40674.0 17 53043.0 18 51589.0 19 33325.0 20 32796.0 21 35348.0 22 39629.0 23 46470.0 24 54368.0 25 60993.0 26 69521.0 27 80751.0 28 96062.0 29 115435.0 30 140598.0 31 176484.0 32 226600.0 33 307855.0 34 449758.0 35 732017.0 36 1343942.0 37 2273034.0 38 1838760.0 39 319414.0 40 334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.26477016518919 17.063382422485763 11.752609197388157 25.919238214936886 2 16.447786776217505 20.73518029768211 36.08345517338251 26.733577752717874 3 19.328421377811942 24.97733704797221 28.481399090661135 27.212842483554716 4 13.681465895071101 16.512170102287147 33.58319870906302 36.22316529357874 5 14.430937438736905 35.90238500970026 33.59275416355812 16.073923388004715 6 33.741932126937414 33.30939233800292 17.335560353348185 15.613115181711473 7 28.11847459947811 30.24199765814216 21.687353456342418 19.952174286037312 8 27.895030081221854 31.4850917203233 20.43759048078272 20.18228771767213 9 27.746723356359542 13.674282165930544 18.535470303825964 40.04352417388395 10-11 26.015629617841636 24.20971537045036 26.41568531611613 23.35896969559188 12-13 27.49670329695316 22.021834241350156 26.28849957406335 24.19296288763333 14-15 25.461932165747488 22.679759542214764 23.796991091015084 28.061317201022668 16-17 24.458440387377934 25.259428802254092 25.798120135141982 24.484010675225992 18-19 25.237538283146076 24.726477135944343 26.768830041833457 23.267154539076117 20-21 25.291612216115322 24.437100585068418 25.886590977914192 24.384696220902068 22-23 24.492078998812445 24.74275193428119 24.66798006431869 26.09718900258768 24-25 24.36849956099105 25.343739774426734 24.489324323948093 25.79843634063413 26-27 25.1153649958813 24.439396403205276 24.877288507545302 25.56795009336812 28-29 24.361262515657092 24.13888192828658 26.454634058955524 25.045221497100805 30-31 25.257629212012617 24.28631969525565 25.84266973554779 24.613381357183947 32-33 23.544440116724534 24.885608283956877 25.719290825070235 25.85066077424835 34-35 23.87840427015801 25.183642374266736 26.311627393456188 24.62632596211907 36-37 24.730336798527127 24.778552042279035 25.553903137562973 24.93720802163087 38-39 24.99330657781163 24.006023966425477 25.44665961330982 25.55400984245308 40-41 24.289345939632184 24.507837988562958 25.9712902276509 25.231525844153957 42-43 25.389564994532744 24.031900677242426 25.725015262836752 24.853519065388078 44-45 24.767005907012464 24.10729440090174 25.93795436509908 25.187745326986715 46-47 23.942750832032697 24.10888462957243 27.161324679238696 24.78703985915618 48-49 24.345006174799238 25.045528951549034 26.34942220892284 24.260042664728886 50-51 23.155403340016868 25.41137037344539 25.83439189583529 25.598834390702446 52-53 23.0911290011626 25.268831095745558 25.02532274467717 26.614717158414674 54-55 23.70840591956286 24.838083566893847 25.10756686115444 26.345943652388854 56-57 24.160922470455276 23.84092619503932 25.352028549845123 26.646122784660275 58-59 23.986326212155646 23.86159003782462 26.211968244265517 25.940115505754218 60-61 23.915616142688748 24.24728644998085 25.69929928984242 26.137798117487982 62-63 22.958670358059543 24.286592783157698 25.986618786000577 26.768118072782176 64-65 23.15137178830945 24.20692459354126 25.945981421235093 26.695722196914197 66-67 24.059029767477195 24.09369110801206 25.347091737525595 26.500187386985154 68-69 24.28025483636813 23.610256057427364 25.249126454449865 26.86036265175464 70-71 23.289832182266483 23.763415107230006 25.812305013636553 27.134447696866953 72-73 24.055647847607364 23.419338166597093 25.95841462133281 26.566599364462736 74-75 24.07546556188868 23.131367646835734 26.177190642098513 26.615976149177072 76-77 23.390834571091354 23.571705257809747 26.742486552713213 26.294973618385686 78-79 23.60530843308062 23.53495941592986 26.656265929171536 26.20346622181799 80-81 23.18132516794119 23.488702432321002 26.774314936356365 26.555657463381444 82-83 23.28360858529199 23.793735741423813 25.901749086085356 27.02090658719884 84-85 23.472533552681657 23.719078873370652 25.831491860215433 26.976895713732258 86-87 23.688823525000323 22.88905837139683 26.352497305311452 27.0696207982914 88-89 23.786320079879495 22.28164951747046 26.85914490814233 27.072885494507716 90-91 24.321034508477876 22.59031916662449 26.272780706204664 26.815865618692975 92-93 24.186586132414696 22.606369614948022 26.386434616858857 26.820609635778432 94-95 23.819062170366568 22.64048338781243 26.823435257446874 26.71701918437413 96-97 24.720440688441943 22.27038323480751 26.14372571595083 26.86545036079972 98-99 24.690459292974246 21.9798670711048 26.025886333085683 27.303787302835275 100 24.331044815429294 22.2201843724924 26.267031170898836 27.18173964117947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1939.0 1 3396.5 2 2912.5 3 826.0 4 608.5 5 578.5 6 636.0 7 792.0 8 984.5 9 1014.0 10 945.0 11 932.0 12 1062.0 13 1225.0 14 1382.0 15 1536.5 16 1741.5 17 2037.5 18 2365.0 19 2790.0 20 3327.5 21 3995.5 22 4886.5 23 6032.0 24 7625.0 25 9803.0 26 12760.5 27 16341.5 28 20234.0 29 25424.0 30 31787.5 31 38882.5 32 47450.5 33 57401.0 34 68586.0 35 78740.0 36 87899.5 37 98623.5 38 108333.0 39 119459.5 40 129926.0 41 140942.5 42 156077.5 43 181377.0 44 214108.0 45 243510.0 46 284151.5 47 335106.0 48 422838.5 49 525658.0 50 622596.5 51 612233.5 52 507041.5 53 452062.5 54 413641.5 55 374768.0 56 344808.5 57 311353.0 58 284837.5 59 259191.5 60 222676.0 61 186156.5 62 152367.5 63 119417.0 64 90650.0 65 72174.0 66 55799.0 67 41786.5 68 32581.5 69 26474.5 70 22732.0 71 19477.5 72 16050.0 73 15479.5 74 13235.0 75 10625.5 76 8070.0 77 5153.0 78 3585.0 79 2556.5 80 1853.5 81 1486.0 82 1177.0 83 838.0 84 599.0 85 397.0 86 268.0 87 177.5 88 122.5 89 98.0 90 72.0 91 66.5 92 60.0 93 46.5 94 39.0 95 32.0 96 20.5 97 18.0 98 15.0 99 9.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08615165458585167 2 0.28065069677815946 3 0.28499457299016334 4 0.29347817457392017 5 0.3183959187978691 6 0.16801614152643585 7 0.1849266361272132 8 0.15135516461929838 9 0.20145151247418341 10-11 0.22241099873993564 12-13 0.239321493340713 14-15 0.16691032447507714 16-17 0.15678784531264 18-19 0.17379474447686913 20-21 0.20556288356256827 22-23 0.16902555401434272 24-25 0.19366542626128358 26-27 0.19164093042879615 28-29 0.22093090514811708 30-31 0.25508647489341624 32-33 0.22361889121141976 34-35 0.1498694001708062 36-37 0.14449909890087456 38-39 0.11178392675067853 40-41 0.10364057656733973 42-43 0.11895756044282585 44-45 0.13380386321440035 46-47 0.19820778245686466 48-49 0.2034533248799763 50-51 0.21006554376143388 52-53 0.13812505599970964 54-55 0.08362245250941078 56-57 0.12087430999851423 58-59 0.1409434717665059 60-61 0.11764759255121635 62-63 0.13840859883339135 64-65 0.11303151521887805 66-67 0.11759655484115364 68-69 0.10302245318991358 70-71 0.12777574257032712 72-73 0.15237591882055257 74-75 0.19065987222425743 76-77 0.17173055264766626 78-79 0.1963647540379335 80-81 0.10141192989460146 82-83 0.141096584896694 84-85 0.17216720861153612 86-87 0.18559579721470204 88-89 0.16730161358555792 90-91 0.17093663271335746 92-93 0.12082894314512517 94-95 0.3593395039815085 96-97 0.35913535314125766 98-99 0.405738453285184 100 0.4388562562592081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8817010.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.873338386964846 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41610933486936 31.64254833446384 2 12.207623907413009 9.979326857369879 3 4.021643688332969 4.931340100351045 4 1.7830485544976724 2.9151658771348754 5 1.0250526151685744 2.0948661202114196 6 0.6894707006432467 1.6908581553173498 7 0.46003488566254597 1.3162213086045749 8 0.33436256345955767 1.0933211360172408 9 0.26252511519205346 0.9657250081489556 >10 1.53356524685121 11.683936018637578 >50 0.1344998461062199 3.820387671595671 >100 0.10574643964821659 8.963208126243993 >500 0.014775917825838486 4.170146566478579 >1k 0.01042609406502187 8.060237410488071 >5k 7.248086717820695E-4 2.0898405627794903 >10k+ 3.902815924980374E-4 4.582870746157379 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 74996 0.8505831341917498 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 74377 0.8435626136297905 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 28471 0.322909920710082 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 27478 0.3116475993562443 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 26940 0.3055457575754139 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 26746 0.30334546518604383 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24579 0.2787679723625129 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 23455 0.2660198865601831 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 21353 0.24217960510422468 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 18450 0.20925461125710418 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17412 0.19748191280263944 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14959 0.1696606899617898 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 12369 0.1402856523923643 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 10620 0.12044899574799167 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9768 0.1107858559761189 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9411 0.10673686431114404 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 9125 0.10349313429382523 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9009 0.10217749554554208 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2683426694537037E-5 0.0 0.0 0.0 0.0 2 1.0207542012541666E-4 0.0 0.0 0.0 0.0 3 1.0207542012541666E-4 0.0 0.0 0.0 0.0 4 1.247588468199537E-4 0.0 0.0 4.536685338907407E-5 0.0 5 8.166033610033333E-4 0.0 0.0 1.0207542012541666E-4 0.0 6 0.0016445484353539352 0.0 0.0 1.1341713347268519E-4 0.0 7 0.001769307282173889 0.0 0.0 1.3610056016722223E-4 0.0 8 0.0019167495556883796 0.0 0.0 1.5878398686175926E-4 1.1341713347268518E-5 9 0.002086875255897407 0.0 0.0 1.814674135562963E-4 1.1341713347268518E-5 10-11 0.0033628180074651154 0.0 0.0 2.495176936399074E-4 1.1341713347268518E-5 12-13 0.008103654186623357 1.701257002090278E-5 0.0 3.345805437444213E-4 1.1341713347268518E-5 14-15 0.01420549596745382 2.2683426694537037E-5 5.670856673634259E-6 3.629348271125926E-4 2.2683426694537037E-5 16-17 0.016876469460735555 2.2683426694537037E-5 1.1341713347268518E-5 4.7635196058527774E-4 2.2683426694537037E-5 18-19 0.01833387962585956 2.8354283368171298E-5 1.1341713347268518E-5 5.89769094057963E-4 2.2683426694537037E-5 20-21 0.02430529170319644 3.402514004180556E-5 1.1341713347268518E-5 0.0010264250579278009 2.2683426694537037E-5 22-23 0.02626740811227389 3.402514004180556E-5 1.1341713347268518E-5 0.002642619209913565 2.2683426694537037E-5 24-25 0.03949184587518898 3.402514004180556E-5 1.1341713347268518E-5 0.007258696542251851 2.2683426694537037E-5 26-27 0.07622198455031808 3.402514004180556E-5 1.1341713347268518E-5 0.017477580268140787 2.8354283368171298E-5 28-29 0.08346366852254902 3.9695996715439816E-5 1.1341713347268518E-5 0.07638076853717984 3.402514004180556E-5 30-31 0.08510254610122933 4.536685338907407E-5 1.1341713347268518E-5 0.24066548637236435 3.402514004180556E-5 32-33 0.08640117227949157 4.536685338907407E-5 1.1341713347268518E-5 0.530848893218903 3.402514004180556E-5 34-35 0.08729149677725215 4.536685338907407E-5 1.1341713347268518E-5 0.9054430016524876 3.402514004180556E-5 36-37 0.08883396979248068 4.536685338907407E-5 1.1341713347268518E-5 1.4557939709720187 3.402514004180556E-5 38-39 0.09038211366438283 4.536685338907407E-5 1.1341713347268518E-5 2.2957612614707257 3.402514004180556E-5 40-41 0.09168641069931871 4.536685338907407E-5 1.1341713347268518E-5 3.3751010830202075 3.402514004180556E-5 42-43 0.09517398755360378 4.536685338907407E-5 1.1341713347268518E-5 4.14771560880616 3.402514004180556E-5 44-45 0.09874095640131972 4.536685338907407E-5 1.1341713347268518E-5 4.939321833592113 4.536685338907407E-5 46-47 0.10021537913646464 4.536685338907407E-5 1.1341713347268518E-5 5.82045387268473 4.536685338907407E-5 48-49 0.1011680830576352 4.536685338907407E-5 1.1341713347268518E-5 6.630354281099828 5.6708566736342595E-5 50-51 0.10265951836280099 4.536685338907407E-5 1.1341713347268518E-5 7.472657964548072 5.6708566736342595E-5 52-53 0.10312452861003901 4.536685338907407E-5 1.1341713347268518E-5 8.367621223067683 7.372113675724537E-5 54-55 0.10465565991192025 6.805028008361112E-5 1.1341713347268518E-5 9.182052645965015 9.073370677814815E-5 56-57 0.10803549048940628 6.805028008361112E-5 1.1341713347268518E-5 10.03170575966229 1.0774627679905092E-4 58-59 0.10912429497074405 6.805028008361112E-5 1.1341713347268518E-5 10.896568110958249 1.1341713347268519E-4 60-61 0.1097254057781493 6.805028008361112E-5 1.1341713347268518E-5 11.678624613105804 1.1341713347268519E-4 62-63 0.11006565717856734 6.805028008361112E-5 1.1341713347268518E-5 12.441042938592561 1.247588468199537E-4 64-65 0.11045127543237447 6.805028008361112E-5 1.1341713347268518E-5 13.22866255113695 1.247588468199537E-4 66-67 0.11088226053957068 9.073370677814815E-5 1.1341713347268518E-5 14.039651764033387 1.247588468199537E-4 68-69 0.1112678787933778 1.0207542012541666E-4 1.1341713347268518E-5 14.94780543517587 1.247588468199537E-4 70-71 0.11180661017737306 1.0207542012541666E-4 1.1341713347268518E-5 15.966319648043951 1.247588468199537E-4 72-73 0.11375738487310325 1.0207542012541666E-4 1.1341713347268518E-5 16.802941133105215 1.247588468199537E-4 74-75 0.11583858927232701 1.0774627679905092E-4 1.1341713347268518E-5 17.578289011807858 1.3042970349358798E-4 76-77 0.11646238350642678 1.1908799014631943E-4 1.1341713347268518E-5 18.407254840359712 1.3610056016722223E-4 78-79 0.11681964747686574 1.247588468199537E-4 1.1341713347268518E-5 19.228638733538922 1.3610056016722223E-4 80-81 0.11763625083786908 1.3042970349358798E-4 1.1341713347268518E-5 20.08405910847328 1.4744227351449075E-4 82-83 0.1178404016781199 1.3610056016722223E-4 1.1341713347268518E-5 20.989003074738488 1.4744227351449075E-4 84-85 0.11870804274918595 1.417714168408565E-4 1.1341713347268518E-5 21.821762706405003 1.4744227351449075E-4 86-87 0.12028454090445628 1.4744227351449075E-4 1.1341713347268518E-5 22.65028620813632 1.4744227351449075E-4 88 0.12069851344163157 1.5878398686175926E-4 1.1341713347268518E-5 23.289732006655317 1.4744227351449075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTACA 8065 0.0 30.219875 2 TACACTC 9080 0.0 28.652962 5 TCTACAC 9650 0.0 27.982573 3 CTACACT 12140 0.0 24.376959 4 TAGTACT 2460 0.0 22.376816 4 CACTCTT 11535 0.0 22.047739 7 GTATAAG 2390 0.0 21.080433 1 CTAGTAC 2835 0.0 20.908386 3 ACACTCT 12775 0.0 20.238867 6 CATGGGG 15660 0.0 20.21952 4 ACTCTTT 14635 0.0 20.043625 8 CTGTGCG 3515 0.0 18.723503 9 GTACTAG 2200 0.0 18.406424 1 CGTATCA 5510 0.0 18.289957 94 CCTAGTA 3120 0.0 18.247276 2 CATGGGT 7815 0.0 18.181322 4 AGTACTT 17935 0.0 18.095123 12-13 CGCCGTA 10045 0.0 17.01795 94 GAGTACT 17115 0.0 16.820093 12-13 TGGTCGC 27845 0.0 16.692465 94 >>END_MODULE