FastQCFastQC Report
Fri 27 May 2016
ERR765247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8817010
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT749760.8503562999248044No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA746360.8465001173867331No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG298980.33909454565663416No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG278830.3162409932618881No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA277240.3144376608396724No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC273540.31024122690118305No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC255860.2901890777032123No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG255470.2897467508826689No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC215490.2444025809202893No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT197550.22405554717528958No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT177960.20183713072799056No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC156260.17722561276441787No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG122740.13920818962437378No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA103610.11751149199104913No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC100690.1141997116936467No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC100250.1137006763063669No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC92790.10523975814930459No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT92260.10463864734189936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.10442315478830125No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA89890.1019506612785967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC28100.024.7520333
GATCTAC37300.024.4555531
TACACTC41050.024.2703325
TCTACAC50050.023.3803373
TAGTACT23350.021.7365154
TCTCGCG12750.021.37778
ATCTACA48050.020.8414962
CTACACT68800.020.6969914
GTACTAG23700.020.2365471
CTAGTAC26200.019.1927853
CCGATCT38600.018.5058614
CGATCTC29750.018.3241585
GACGTTA7150.017.7460177
GATGTAA29150.017.5821741
CATGGGG170550.017.497464
GTCCTAC30500.017.1122741
ATGTAAG30650.016.873492
AGTACTT168850.016.79614312-13
GAGTACT158050.016.6207512-13
TACACTA20950.016.5997185
Produced by FastQC (version 0.11.5)