FastQCFastQC Report
Fri 27 May 2016
ERR765245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139820
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT717970.6445077209506078No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA675710.6065717399383473No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG509890.45771834733415795No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG362990.3258490711699112No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT250720.22506647324642587No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC241600.21687962642125275No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG236100.2119423832701067No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC220430.19787572869220507No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC202250.18155589587623497No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA191400.1718160616598832No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT190710.1711966620645576No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC182950.16423066081857696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175110.15719284512676146No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG127130.11412213123730905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC130650.024.7827873
TACACTC118150.024.589365
CTACACT145300.023.6142224
ATCTACA121500.023.2823832
GTATAAG42900.020.593641
ACACTCT152550.019.2609566
TAGTACT46000.019.2095174
CACTCTT150800.019.111067
GTGATCG32000.019.0993678
GTACAAA58350.018.9260311
GTACTAG40650.018.7278121
CTATTGA59250.018.7261839
ACTCTTT170400.018.2647618
GTATATA23000.017.9799041
GTATTAG25100.017.7861711
CGTTAAC15950.017.3829651
TGATCGC35400.017.2649679
TTTAGGG70050.017.1056392
GGGCTAT71900.016.7386766
GAACAAA73900.016.7241421
Produced by FastQC (version 0.11.5)