##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765245_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11139820
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73754360483383	33.0	33.0	33.0	27.0	33.0
2	31.718984956668958	33.0	33.0	33.0	27.0	33.0
3	31.680413507579118	33.0	33.0	33.0	27.0	33.0
4	35.37733338599726	37.0	37.0	37.0	33.0	37.0
5	35.44724878858007	37.0	37.0	37.0	33.0	37.0
6	35.392504726288216	37.0	37.0	37.0	33.0	37.0
7	35.33329174080012	37.0	37.0	37.0	33.0	37.0
8	35.32200636994135	37.0	37.0	37.0	33.0	37.0
9	35.294155201789614	37.0	37.0	37.0	33.0	37.0
10-11	35.27178262305854	37.0	37.0	37.0	33.0	37.0
12-13	35.24771971180863	37.0	37.0	37.0	33.0	37.0
14-15	36.71364945753163	37.0	37.0	40.0	33.0	40.0
16-17	36.75315593070624	37.0	37.0	40.0	33.0	40.0
18-19	36.80106666894079	37.0	37.0	40.0	33.0	40.0
20-21	36.811976854204104	37.0	37.0	40.0	33.0	40.0
22-23	36.79574315383911	37.0	37.0	40.0	33.0	40.0
24-25	36.76337319633531	37.0	37.0	40.0	33.0	40.0
26-27	36.743383959525374	37.0	37.0	40.0	33.0	40.0
28-29	36.70708795115181	37.0	37.0	40.0	33.0	40.0
30-31	36.5978483045507	37.0	37.0	40.0	33.0	40.0
32-33	36.468598280762166	37.0	37.0	40.0	33.0	40.0
34-35	36.36112769326614	37.0	37.0	40.0	33.0	40.0
36-37	36.2922408530838	37.0	37.0	40.0	33.0	40.0
38-39	36.18336117639244	37.0	37.0	40.0	33.0	40.0
40-41	36.017527617142825	37.0	37.0	40.0	27.0	40.0
42-43	35.89869818363313	37.0	37.0	40.0	27.0	40.0
44-45	35.722539412665554	37.0	37.0	40.0	27.0	40.0
46-47	35.49572852164577	37.0	35.0	40.0	27.0	40.0
48-49	35.251818925260906	37.0	33.0	40.0	27.0	40.0
50-51	35.03876359761648	37.0	33.0	40.0	27.0	40.0
52-53	34.85941868899138	37.0	33.0	40.0	27.0	40.0
54-55	34.72909014687849	37.0	33.0	40.0	27.0	40.0
56-57	34.56846542403737	37.0	33.0	40.0	27.0	40.0
58-59	34.389590361424155	37.0	33.0	40.0	27.0	40.0
60-61	34.102286931027606	37.0	33.0	37.0	27.0	40.0
62-63	33.86800949207438	37.0	33.0	37.0	27.0	40.0
64-65	33.653766577915974	37.0	33.0	37.0	27.0	40.0
66-67	33.427154971983384	37.0	33.0	37.0	24.5	40.0
68-69	33.181318279828574	37.0	33.0	37.0	22.0	40.0
70-71	32.93256704327359	37.0	33.0	37.0	22.0	38.5
72-73	32.655888694790406	37.0	33.0	37.0	22.0	37.0
74-75	32.45607164209116	35.0	33.0	37.0	22.0	37.0
76-77	32.240045440590606	33.0	33.0	37.0	22.0	37.0
78-79	32.03417757198949	33.0	33.0	37.0	22.0	37.0
80-81	31.84937871527547	33.0	33.0	37.0	22.0	37.0
82-83	31.66346305416066	33.0	33.0	37.0	22.0	37.0
84-85	31.492609530495105	33.0	33.0	37.0	22.0	37.0
86-87	31.346448551233323	33.0	33.0	37.0	22.0	37.0
88-89	31.22005032397292	33.0	33.0	37.0	22.0	37.0
90-91	31.08190370221422	33.0	27.0	37.0	18.5	37.0
92-93	30.901464206782514	33.0	27.0	37.0	15.0	37.0
94-95	30.80902770421784	33.0	27.0	37.0	15.0	37.0
96-97	30.62455053133713	33.0	27.0	37.0	15.0	37.0
98-99	30.49524866649551	33.0	27.0	37.0	15.0	37.0
100	30.401136194301166	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	9.0
7	355.0
8	2341.0
9	3638.0
10	4098.0
11	7852.0
12	17411.0
13	30677.0
14	37036.0
15	37745.0
16	39940.0
17	44973.0
18	52998.0
19	62647.0
20	74213.0
21	92936.0
22	107500.0
23	103696.0
24	98860.0
25	100679.0
26	110871.0
27	131307.0
28	160397.0
29	203784.0
30	262865.0
31	346496.0
32	468809.0
33	644374.0
34	914995.0
35	1376347.0
36	2160135.0
37	2644433.0
38	795006.0
39	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99848356737072	19.271839381869412	12.908999765922205	24.820677284837657
2	15.312363097076293	21.472931823241716	37.74799627983137	25.46670879985062
3	18.27376163762529	26.961458826262845	29.389394495600634	25.375385040511233
4	12.97324598951989	17.91463293764795	36.06083447289191	33.05128659994024
5	13.449575068812342	37.65888740147238	33.58098803380345	15.310549495911838
6	30.793085648366347	37.3162314898354	17.301050798310463	14.58963206348779
7	25.81563426489853	33.00240348704925	22.090498667003747	19.09146358104847
8	26.642649721217776	33.37896660700137	20.306770871768794	19.671612800012067
9	27.03909621480699	15.005884766310587	19.603812933558252	38.35120608532417
10-11	24.568793980822996	26.47546771602863	26.716464308188296	22.239273994960072
12-13	26.311625573971163	24.256082370249473	26.594642205485986	22.837649850293378
14-15	24.321613824004412	24.10845416308651	24.966277589497093	26.60365442341198
16-17	22.92241065766938	27.06969167030579	26.143570116717225	23.864327555307604
18-19	23.716763581298267	26.47661529199137	27.95775767592342	21.848863450786943
20-21	24.72579112201811	25.576792781632257	26.85951405510278	22.837902041246856
22-23	24.088253198898382	25.863902634259116	25.829849095740983	24.217995071101516
24-25	23.811464363198034	26.28383896576419	25.80290333461781	24.101793336419966
26-27	24.09550910057726	25.69033023073749	26.01816367578139	24.19599699290386
28-29	23.643274389731562	25.310933304415705	27.366695548385195	23.679096757467537
30-31	24.630074774992824	25.261866917654125	26.783532252476395	23.32452605487666
32-33	23.114013637350066	26.144801244013355	26.69275630143602	24.048428817200556
34-35	23.09191962991192	26.172716148800113	27.088891522286175	23.64647269900179
36-37	23.997242446466018	26.00379446404699	26.355661742374153	23.643301347112843
38-39	24.348359498063427	25.045998852783608	26.510510935923982	24.095130713228983
40-41	23.56514079607002	25.47138819802111	26.84212553485017	24.1213454710587
42-43	24.531384600910712	25.330196961922226	26.4589773679257	23.679441069241356
44-45	24.087802203735993	25.388796696124118	26.779594038333208	23.743807061806688
46-47	23.424576689385294	25.487482968211623	27.176460886736187	23.911479455666896
48-49	24.153134468085373	25.607424300994335	26.88180157310476	23.35763965781553
50-51	23.405626203547016	25.99010229869063	26.01157649509155	24.59269500267081
52-53	22.85263643879639	26.363750474974157	25.417842579377247	25.365770506852197
54-55	23.350646637055856	26.00999846320466	25.298174437090903	25.341180462648577
56-57	23.69427533316278	25.060850209209978	25.634699071749534	25.610175385877703
58-59	23.295628157668745	24.88798357324333	26.750847375534025	25.065540893553905
60-61	23.589184240357874	25.480020077463962	25.92232308934391	25.00847259283426
62-63	22.60485903911414	25.536286124011642	26.016179139948147	25.842675696926072
64-65	22.187600016339275	25.50325191975261	26.114619117580386	26.19452894632773
66-67	23.370087475330813	25.325818200819672	25.559641809848426	25.744452514001086
68-69	23.294660436235233	24.751327440897555	25.859562671329122	26.094449451538086
70-71	22.730837924080202	25.006901269429598	26.024909206226088	26.23735160026411
72-73	23.565202480381302	24.778904190020118	26.07371117221987	25.582182157378703
74-75	23.37430641327277	24.722494614532103	26.26928295546596	25.633916016729163
76-77	22.63092595916847	25.03478085335401	26.593301544084497	25.74099164339302
78-79	22.858890427800667	25.049096482437278	26.510390392498508	25.581622697263544
80-81	22.651828749900126	25.046718610148282	26.571240942882817	25.73021169706878
82-83	22.747775497479694	24.865463385640744	26.325593261640616	26.06116785523894
84-85	22.945199776224666	24.88128824424028	25.893205946161142	26.28030603337391
86-87	22.631221682594056	24.513758992188176	26.39686185879469	26.45815746642308
88-89	22.885504790083736	23.914035232609912	26.75853345877081	26.441926518535542
90-91	23.036429961855763	24.111266825590892	26.493985627338567	26.35831758521478
92-93	22.734625558459157	24.39020241201752	26.473529199753354	26.401642829769973
94-95	22.03931713111906	24.392589774113063	26.87238578189424	26.695707312873633
96-97	22.55470170520214	24.395414253570664	26.322753638214913	26.72713040301228
98-99	22.546104644654292	24.401130450184432	26.227258514666886	26.82550639049439
100	22.440442957899283	24.396978299810208	26.313043320570102	26.849535421720404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	526.0
1	474.5
2	424.5
3	416.0
4	333.0
5	296.5
6	425.0
7	802.0
8	1227.0
9	1211.5
10	915.0
11	874.5
12	1049.0
13	1345.5
14	1722.5
15	2137.0
16	2780.0
17	3569.5
18	4428.0
19	5464.5
20	6741.5
21	8374.0
22	10570.0
23	13246.5
24	16290.0
25	20621.0
26	27269.0
27	34990.0
28	43438.5
29	57283.0
30	73989.5
31	88905.0
32	109232.5
33	131196.5
34	153635.0
35	172012.0
36	188643.5
37	217041.5
38	232921.5
39	234626.5
40	236861.0
41	240297.0
42	250935.5
43	273224.0
44	304203.0
45	338802.0
46	378567.5
47	429068.5
48	505789.5
49	575900.5
50	653452.0
51	673030.5
52	589857.5
53	521924.0
54	469798.0
55	410670.5
56	369710.5
57	327372.5
58	289093.0
59	262425.5
60	224891.5
61	185780.0
62	152071.0
63	121535.0
64	94047.5
65	72267.0
66	57489.0
67	46096.0
68	37483.0
69	31283.0
70	26151.5
71	21994.0
72	18920.5
73	17078.5
74	14006.5
75	11212.5
76	8975.0
77	6773.0
78	5263.5
79	3931.0
80	2864.0
81	2034.0
82	1589.0
83	1275.0
84	916.5
85	629.5
86	420.0
87	255.0
88	151.0
89	92.0
90	50.0
91	41.0
92	35.0
93	15.0
94	8.5
95	8.0
96	5.5
97	3.5
98	3.5
99	4.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022415083906203153
2	0.004847475093852503
3	0.009488483655929808
4	0.009012712952273915
5	0.010709329235122291
6	0.006014459838668847
7	0.009012712952273915
8	0.009138388232484905
9	0.007989357099127275
10-11	0.028258984436014226
12-13	0.03026978891939008
14-15	0.013065740739078369
16-17	0.004142795844098019
18-19	0.011876313979938635
20-21	0.0016382670456075593
22-23	7.630284869953016E-5
24-25	0.003478512220125639
26-27	0.0015215685711259248
28-29	0.0011849383562750565
30-31	0.027006720036768996
32-33	0.020817212486377697
34-35	0.007630284869953016
36-37	0.008626710305911585
38-39	0.00613564671601516
40-41	0.014847636676355632
42-43	0.003662536737577447
44-45	0.0037567931977356903
46-47	0.013074717544807725
48-49	0.017141210540206214
50-51	0.007809820984540146
52-53	0.02129298319003359
54-55	0.027006720036768996
56-57	0.014439192015669913
58-59	0.010444513466106274
60-61	0.008644663917370298
62-63	0.015372779811522987
64-65	0.009111457815296837
66-67	0.017388072697763518
68-69	0.005597038372253771
70-71	0.005071895237086416
72-73	0.010861934932521352
74-75	0.0031553472138688057
76-77	0.010309861380165927
78-79	0.002464133172708356
80-81	0.007091676526191627
82-83	0.0017459887143598369
84-85	0.008703013154611116
86-87	0.0032271616597036577
88-89	0.0012567528021099083
90-91	0.0011624963419516652
92-93	1.5260569739906031E-4
94-95	0.0033707905513733617
96-97	7.630284869953015E-4
98-99	0.00485645189958186
100	0.007118606943379696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.113982E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.98913039695051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23839441362162	28.582411762362646
2	13.193400529188635	10.024116261650857
3	4.631990650980734	5.278958905126884
4	2.102562503975228	3.1949808452501482
5	1.1643346858986519	2.211603110414815
6	0.7318320337900818	1.6680997536190147
7	0.4879418295247138	1.2975540051558665
8	0.3410904303619205	1.0366183068936772
9	0.2718728037643555	0.9295390254229775
>10	1.5267761883905036	10.867956790340056
>50	0.14850499002637796	3.9107236481537107
>100	0.12569330373802545	10.06163569515717
>500	0.02068556295290402	5.570498640057383
>1k	0.013730275479437691	9.26070803640804
>5k	7.85266882643628E-4	1.9460693374256897
>10k+	4.0453142439217203E-4	4.158525876561093
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	71797	0.6445077209506078	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	67571	0.6065717399383473	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	50989	0.45771834733415795	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	36299	0.3258490711699112	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	25072	0.22506647324642587	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	24160	0.21687962642125275	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	23610	0.2119423832701067	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	22043	0.19787572869220507	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	20225	0.18155589587623497	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	19140	0.1718160616598832	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	19071	0.1711966620645576	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	18295	0.16423066081857696	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17511	0.15719284512676146	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	12713	0.11412213123730905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.976805729356489E-4	0.0	0.0	1.7953611458712977E-5	0.0
2	0.0014811729453438206	0.0	0.0	1.7953611458712977E-5	0.0
3	0.0015888946140960986	0.0	0.0	1.7953611458712977E-5	0.0
4	0.0017594539229538718	0.0	0.0	2.6930417188069465E-5	0.0
5	0.0058079933068936485	0.0	0.0	1.3465208594034734E-4	0.0
6	0.012971484278920127	0.0	0.0	3.141882005274771E-4	0.0
7	0.014793775841979493	0.0	0.0	3.5907222917425953E-4	0.0
8	0.018097240350382682	0.0	0.0	4.3986348073846794E-4	0.0
9	0.020377349005639228	0.0	0.0	4.757707036558939E-4	0.0
10-11	0.04178254226728978	0.0	0.0	8.393313356948318E-4	0.0
12-13	0.07880288909515594	0.0	0.0	0.001638267045607559	0.0
14-15	0.12345352079297511	0.0	0.0	0.0025224824099491735	8.976805729356488E-6
16-17	0.14414056959627713	0.0	0.0	0.0030431371422518496	8.976805729356488E-6
18-19	0.15587774308741076	0.0	0.0	0.0032855108969444746	8.976805729356488E-6
20-21	0.19651574262420757	8.976805729356488E-6	0.0	0.004156261052692054	8.976805729356488E-6
22-23	0.2086209651502448	1.3465208594034732E-5	0.0	0.0054085254519372844	8.976805729356488E-6
24-25	0.2667547590535574	1.7953611458712977E-5	0.0	0.011059424658567194	8.976805729356488E-6
26-27	0.41626794687885443	1.7953611458712977E-5	0.0	0.03024734690506669	8.976805729356488E-6
28-29	0.4462415012091757	1.7953611458712977E-5	0.0	0.0566301789436454	8.976805729356488E-6
30-31	0.4569553188471627	1.7953611458712977E-5	0.0	0.13391149946767542	8.976805729356488E-6
32-33	0.46595007818797796	1.7953611458712977E-5	0.0	0.26964080209554553	8.976805729356488E-6
34-35	0.47286221859958244	1.7953611458712977E-5	0.0	0.4491589630712166	8.976805729356488E-6
36-37	0.486538382128257	2.244201432339122E-5	0.0	0.7362057914759843	8.976805729356488E-6
38-39	0.49915528258086755	2.6930417188069465E-5	0.0	1.1873935126420356	1.3465208594034732E-5
40-41	0.5107622923889255	3.590722291742595E-5	0.0	1.826618383420917	1.7953611458712977E-5
42-43	0.5398471429520405	3.590722291742595E-5	0.0	2.2999473959184256	1.7953611458712977E-5
44-45	0.5732588138767054	4.488402864678244E-5	0.0	2.7766157801472557	2.6930417188069465E-5
46-47	0.5845381702756418	4.488402864678244E-5	0.0	3.3317683768678488	2.6930417188069465E-5
48-49	0.5932905558617644	4.488402864678244E-5	0.0	3.882948737053202	2.6930417188069465E-5
50-51	0.6104407432076999	4.488402864678244E-5	0.0	4.515149257348861	2.6930417188069465E-5
52-53	0.6155979180992153	7.630284869953016E-5	0.0	5.2178670750514815	2.6930417188069465E-5
54-55	0.6328333850995798	8.527965442888663E-5	0.0	5.821656005213729	2.6930417188069465E-5
56-57	0.67070204006887	9.425646015824313E-5	0.0	6.4543637150330975	2.6930417188069465E-5
58-59	0.6812408099951346	1.1669847448163435E-4	0.0	7.131156517789336	2.6930417188069465E-5
60-61	0.6881753924210625	1.1669847448163435E-4	0.0	7.770206340856495	2.6930417188069465E-5
62-63	0.6939968509365502	1.1669847448163435E-4	0.0	8.433933402873656	2.6930417188069465E-5
64-65	0.698224926435077	1.2118687734631259E-4	0.0	9.116763107482885	2.6930417188069465E-5
66-67	0.7047151569774017	1.3465208594034734E-4	0.0	9.80335858209558	2.6930417188069465E-5
68-69	0.7100518679835042	1.436288916697038E-4	0.0	10.575538922531962	2.6930417188069465E-5
70-71	0.7161201886565491	1.436288916697038E-4	0.0	11.496536748349614	2.6930417188069465E-5
72-73	0.7351061327741382	1.436288916697038E-4	0.0	12.302016549639042	2.6930417188069465E-5
74-75	0.7535669337565598	1.436288916697038E-4	0.0	13.06538615525206	2.6930417188069465E-5
76-77	0.7591639721288135	1.436288916697038E-4	0.0	13.901423003244219	2.6930417188069465E-5
78-79	0.7627367408090975	1.436288916697038E-4	0.0	14.72630617011765	2.6930417188069465E-5
80-81	0.7707620051311421	1.436288916697038E-4	0.0	15.636926808512166	2.6930417188069465E-5
82-83	0.7736121409502128	1.436288916697038E-4	0.0	16.652881285334953	2.6930417188069465E-5
84-85	0.7844157266454934	1.436288916697038E-4	0.0	17.586276079864845	2.6930417188069465E-5
86-87	0.8046808655795157	1.4811729453438206E-4	0.0	18.496263853455442	2.6930417188069465E-5
88	0.8087653121863728	1.5260569739906031E-4	0.0	19.200489774520594	2.6930417188069465E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACAC	13065	0.0	24.782787	3
TACACTC	11815	0.0	24.58936	5
CTACACT	14530	0.0	23.614222	4
ATCTACA	12150	0.0	23.282383	2
GTATAAG	4290	0.0	20.59364	1
ACACTCT	15255	0.0	19.260956	6
TAGTACT	4600	0.0	19.209517	4
CACTCTT	15080	0.0	19.11106	7
GTGATCG	3200	0.0	19.099367	8
GTACAAA	5835	0.0	18.926031	1
GTACTAG	4065	0.0	18.727812	1
CTATTGA	5925	0.0	18.726183	9
ACTCTTT	17040	0.0	18.264761	8
GTATATA	2300	0.0	17.979904	1
GTATTAG	2510	0.0	17.786171	1
CGTTAAC	1595	0.0	17.382965	1
TGATCGC	3540	0.0	17.264967	9
TTTAGGG	7005	0.0	17.105639	2
GGGCTAT	7190	0.0	16.738676	6
GAACAAA	7390	0.0	16.724142	1
>>END_MODULE