FastQCFastQC Report
Fri 27 May 2016
ERR765244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12495644
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA918400.7349761244798587No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT773060.6186635918885014No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG434460.34768916271942446No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT373120.2986000561475663No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG352590.2821703307168482No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG334030.2673171546820636No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA312350.24996710853798332No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC292620.23417760621221284No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC263160.21060139037251702No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC224370.17955857257136967No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT221230.1770456968844503No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC220830.1767255853319765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177910.1423776157515371No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG135020.10805365453753324No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC132910.10636506609823392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC76000.020.9942461
CATGGGG247300.018.8020524
TACACTC95850.018.6089215
TCTACAC108800.018.5141873
TACGTAC24200.018.478697
GTATAAG42300.018.0255621
CTACACT127600.017.2610654
GTACTAG37650.017.2515561
ATACGTA27000.017.0655636
TATACGT27950.016.6696875
TCTAACG18100.016.6413172
ATCTACA101450.016.5616072
CTAACGC17050.015.734043
GTATTAG36550.014.8089631
GTATAAA80750.014.8048961
TAATACT45600.014.7585464
TTATACG32250.014.7388644
ATTGCGT19750.014.521826
CGTTAAC9200.014.3246611
TATACAG56700.014.2764275
Produced by FastQC (version 0.11.5)