##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765244_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12495644
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80764712887147	33.0	33.0	33.0	27.0	33.0
2	31.78077264365086	33.0	33.0	33.0	27.0	33.0
3	31.821058362418135	33.0	33.0	33.0	27.0	33.0
4	35.60126280806335	37.0	37.0	37.0	33.0	37.0
5	35.5544024781756	37.0	37.0	37.0	33.0	37.0
6	35.51195424581558	37.0	37.0	37.0	33.0	37.0
7	35.45082662406195	37.0	37.0	37.0	33.0	37.0
8	35.44297748879529	37.0	37.0	37.0	33.0	37.0
9	35.28971471978555	37.0	37.0	37.0	33.0	37.0
10-11	35.36639248045159	37.0	37.0	37.0	33.0	37.0
12-13	35.35263368578683	37.0	37.0	37.0	33.0	37.0
14-15	36.820590599412085	40.0	37.0	40.0	33.0	40.0
16-17	36.96751840081232	40.0	37.0	40.0	33.0	40.0
18-19	37.03047053837321	40.0	37.0	40.0	33.0	40.0
20-21	37.0260790880406	40.0	37.0	40.0	33.0	40.0
22-23	36.96392931008598	40.0	37.0	40.0	33.0	40.0
24-25	36.91127103973193	40.0	37.0	40.0	33.0	40.0
26-27	36.891364302632184	40.0	37.0	40.0	33.0	40.0
28-29	36.810466551383826	40.0	37.0	40.0	33.0	40.0
30-31	36.746263297834034	37.0	37.0	40.0	33.0	40.0
32-33	36.60437653313427	37.0	37.0	40.0	33.0	40.0
34-35	36.54361195789509	37.0	37.0	40.0	33.0	40.0
36-37	36.4661027074715	37.0	37.0	40.0	33.0	40.0
38-39	36.36771706204178	37.0	37.0	40.0	33.0	40.0
40-41	36.26991393960968	37.0	37.0	40.0	33.0	40.0
42-43	36.135331880453705	37.0	37.0	40.0	33.0	40.0
44-45	35.926268225951375	37.0	37.0	40.0	33.0	40.0
46-47	35.71833624581494	37.0	37.0	40.0	33.0	40.0
48-49	35.48690503666718	37.0	37.0	40.0	27.0	40.0
50-51	35.32423302872586	37.0	37.0	40.0	27.0	40.0
52-53	35.13527105925873	37.0	35.0	40.0	27.0	40.0
54-55	34.944320796911306	37.0	33.0	40.0	27.0	40.0
56-57	34.779481393676065	37.0	33.0	40.0	27.0	40.0
58-59	34.56437159221245	37.0	33.0	40.0	27.0	40.0
60-61	34.32708446239346	37.0	33.0	38.5	27.0	40.0
62-63	34.048707493587365	37.0	33.0	37.0	27.0	40.0
64-65	33.79483850532233	37.0	33.0	37.0	27.0	40.0
66-67	33.56015896419584	37.0	33.0	37.0	27.0	40.0
68-69	33.29561225495861	37.0	33.0	37.0	27.0	40.0
70-71	33.01283663330997	37.0	33.0	37.0	27.0	38.5
72-73	32.720994852286125	37.0	33.0	37.0	27.0	37.0
74-75	32.44324898340574	37.0	33.0	37.0	24.5	37.0
76-77	32.187154939753405	37.0	33.0	37.0	22.0	37.0
78-79	31.995506914249475	33.0	33.0	37.0	22.0	37.0
80-81	31.7684110958987	33.0	33.0	37.0	22.0	37.0
82-83	31.59447192157523	33.0	33.0	37.0	22.0	37.0
84-85	31.39217218416274	33.0	33.0	37.0	22.0	37.0
86-87	31.188803034081317	33.0	33.0	37.0	22.0	37.0
88-89	30.996052184265174	33.0	33.0	37.0	22.0	37.0
90-91	30.806493446836352	33.0	33.0	37.0	15.0	37.0
92-93	30.6019310009152	33.0	33.0	37.0	15.0	37.0
94-95	30.330143728486505	33.0	33.0	37.0	15.0	37.0
96-97	30.086241053282247	33.0	33.0	37.0	4.0	37.0
98-99	29.775029962441312	33.0	33.0	37.0	2.0	37.0
100	29.469176218528634	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87000.0
3	21664.0
4	12723.0
5	9894.0
6	11837.0
7	18663.0
8	22167.0
9	20773.0
10	21329.0
11	24057.0
12	28907.0
13	31530.0
14	34552.0
15	40185.0
16	46702.0
17	53097.0
18	49859.0
19	38800.0
20	34455.0
21	36847.0
22	43371.0
23	54035.0
24	67589.0
25	84034.0
26	102195.0
27	124983.0
28	157332.0
29	202177.0
30	265196.0
31	353598.0
32	483375.0
33	673547.0
34	956488.0
35	1427437.0
36	2354572.0
37	3376060.0
38	1123953.0
39	661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.811903732507844	18.25718818414544	12.852118058662453	25.07879002468426
2	15.151267425062882	21.456661674141575	37.37280550818626	26.01926539260928
3	18.34179541001332	26.3698683880985	29.51039949884094	25.777936703047246
4	13.186014818695918	17.795661935298533	35.79810121296937	33.22022203303617
5	13.81709306859134	37.53590102481908	33.63997707460269	15.007028831986888
6	31.867553538482476	35.841275558597175	17.730336539038767	14.560834363881586
7	26.729935865558037	32.091849594340275	22.27884054866816	18.89937399143353
8	26.952376553751524	33.199504461233666	20.557246582084833	19.290872402929978
9	27.310553696704243	14.936038165413926	19.269119422022136	38.4842887158597
10-11	24.841174485767368	25.972672578532492	27.334349153871756	21.851803781828387
12-13	26.278563721481913	23.574990141124026	26.92091572346217	23.225530413931892
14-15	24.424194223269875	24.026424149563276	24.772466137640496	26.776915489526353
16-17	23.639290815833263	26.640585433565683	26.090356890638095	23.629766859962952
18-19	24.3841052903429	26.146967370151135	27.136865393220173	22.332061946285783
20-21	24.847170522729193	25.633149548188516	26.45615292063752	23.06352700844477
22-23	24.084290701374805	25.921576125966627	25.45362661173673	24.54050656092184
24-25	23.97415851475922	26.371694007927882	25.315706017232888	24.33844146008001
26-27	24.35602758849404	25.585748121505382	25.3625383373598	24.695685952640776
28-29	24.034996403141676	25.350600654132844	26.583523980029174	24.03087896269631
30-31	24.844741895655797	25.27682846918494	26.224642763510225	23.653786871649032
32-33	23.423922768606403	25.84564669095887	26.253044661003464	24.477385879431264
34-35	23.558481659688766	25.93002809619096	26.485769761046328	24.025720483073943
36-37	24.298600436000026	25.74842195976893	25.882224614078435	24.070752990152606
38-39	24.64490825762962	25.253524348164845	25.766739193274073	24.33482820093146
40-41	24.030774244208622	25.698983581798586	26.03515273002336	24.235089443969436
42-43	25.03990190501586	25.383437620341937	25.620864358811758	23.955796115830445
44-45	24.759164139076045	25.282636589828794	25.907244676734294	24.050954594360867
46-47	24.16386029339089	25.308723735943556	26.688907970190805	23.838508000474746
48-49	24.557515395605517	25.609961933951197	26.242156540423288	23.590366130019998
50-51	23.71358307341	26.010307781318115	25.92057956170902	24.355529583562866
52-53	23.66655171842552	26.205942165162295	25.004031458097764	25.12347465831442
54-55	24.07710198751438	25.883017591323405	24.99437168248398	25.04550873867824
56-57	24.41363240385004	25.081030833693813	25.18320823597722	25.32212852647893
58-59	24.11859022780185	25.09548588356883	26.14412476525974	24.641799123369573
60-61	24.366881975489026	25.387155510119356	25.7117694450726	24.534193069319016
62-63	23.26941686393927	25.591522688913415	26.00985971520158	25.12920073194574
64-65	23.274995126189282	25.583756877355075	25.849303533248673	25.291944463206974
66-67	24.255183065391865	25.521963869577196	25.335095894482578	24.88775717054836
68-69	24.39586402998785	24.905011514659147	25.551587445606327	25.147537009746674
70-71	23.764990585080675	25.348492864644356	25.54497813749215	25.34153841278281
72-73	24.560128038997505	25.161041098731967	25.39892144158633	24.8799094206842
74-75	24.681982062622726	24.937999371700826	25.644637875476484	24.73538069019997
76-77	24.025068255785776	25.340770751791585	26.07774357208	24.55641742034264
78-79	24.203299485591526	25.43838880432811	25.83896134119695	24.51935036888342
80-81	23.83161742142129	25.366336792926848	25.82860605093481	24.973439734717058
82-83	23.77197891184867	25.768778996358133	25.100005862043506	25.359236229749694
84-85	23.85377693344458	25.67777592957069	24.878226072254993	25.59022106472974
86-87	24.024659201570596	24.82783266567936	25.407067652788246	25.740440479961794
88-89	24.093413461753922	24.57256181531943	25.762363864167316	25.571660858759333
90-91	24.358725398041514	24.937576649943747	25.553577202781685	25.150120749233057
92-93	23.625741028124267	25.09152549887414	25.726178851586624	25.556554621414975
94-95	23.211106092896845	25.25547510095861	25.965585542709203	25.567833263435347
96-97	24.05521745690998	25.071966254397992	25.459518254145745	25.413298034546283
98-99	24.082664115907747	24.825812069061758	25.30317505063271	25.788348764397785
100	23.644780540529638	25.117591081620276	25.591572671828626	25.646055706021453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2452.0
1	2204.0
2	2151.0
3	2301.0
4	1881.5
5	1689.0
6	2243.0
7	4038.0
8	6510.5
9	6243.0
10	3722.0
11	2338.0
12	2290.0
13	2519.0
14	2844.0
15	3304.0
16	3834.0
17	4446.0
18	5403.5
19	6536.5
20	7959.0
21	10033.0
22	12658.0
23	15579.5
24	18888.0
25	23853.5
26	30892.5
27	38838.5
28	46542.0
29	57934.0
30	72034.5
31	86448.0
32	102609.0
33	117249.0
34	134456.5
35	151851.0
36	167107.0
37	187115.0
38	206087.5
39	222295.5
40	239190.5
41	258213.5
42	281322.0
43	314368.0
44	350237.5
45	388744.5
46	437545.5
47	494518.0
48	588371.5
49	680281.0
50	793996.5
51	784361.5
52	637065.5
53	565829.5
54	524023.0
55	472701.5
56	429909.0
57	399041.5
58	375514.5
59	339264.5
60	284248.5
61	229991.5
62	183260.5
63	141008.0
64	104761.0
65	81673.5
66	64852.0
67	49738.0
68	39032.5
69	32257.0
70	27557.0
71	23370.0
72	19887.0
73	19297.0
74	15805.0
75	11938.0
76	9176.5
77	6620.5
78	5062.0
79	3608.0
80	2670.0
81	2098.0
82	1576.5
83	1174.0
84	806.0
85	513.0
86	368.5
87	228.5
88	116.5
89	70.0
90	54.5
91	42.0
92	27.0
93	18.5
94	17.0
95	14.5
96	11.0
97	9.0
98	6.5
99	9.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008338905941942648
2	0.05046558624749553
3	0.04958527947819256
4	0.04706440100246134
5	0.07945168732399867
6	0.018358397534372777
7	0.016365703120223337
8	0.020607181190501266
9	0.006170150173932612
10-11	0.018370401717590544
12-13	0.034908164797268555
14-15	0.009923458126687988
16-17	0.006690331446702546
18-19	0.002937023493947171
20-21	0.009307243388175911
22-23	0.013348651738157713
24-25	0.0
26-27	0.0
28-29	1.3604740980136757E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	2.000697202961288E-5
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.881352573744899E-4
46-47	0.004585597989187272
48-49	0.004161450182159479
50-51	0.0010603695175694825
52-53	0.00132046015395445
54-55	0.006330205950169515
56-57	0.005673977267598212
58-59	0.01425696826830214
60-61	0.007646664709718043
62-63	0.0036452703037954666
64-65	0.002304803177811404
66-67	0.001528532663062424
68-69	4.041408349981801E-4
70-71	4.081422294041027E-4
72-73	6.082119497002315E-4
74-75	3.6012549653303185E-5
76-77	0.0
78-79	2.3608226994943198E-4
80-81	3.6012549653303185E-5
82-83	1.2004183217767727E-5
84-85	4.041408349981801E-4
86-87	1.9606832589020624E-4
88-89	2.881003972264255E-4
90-91	9.563332630154956E-4
92-93	6.60230076977225E-4
94-95	4.2014641262187047E-4
96-97	2.4408505876127713E-4
98-99	1.32046015395445E-4
100	8.803067693029667E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2495644E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.205129542744736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.03508082736522	23.587290609520053
2	14.09935086539875	9.36341543908351
3	5.357833363269605	5.3372265268742085
4	2.6589011586618043	3.5315662965887715
5	1.5744797806873914	2.6140402540078576
6	1.0120554848660823	2.0163260087654162
7	0.6813997208686949	1.5838176201284586
8	0.49667049940987396	1.319360661837167
9	0.375414922097517	1.1219131008474725
>10	2.2576909366604467	14.286912628317333
>50	0.23219177692605408	5.397658886951622
>100	0.1846178155937689	12.323527288735155
>500	0.021858560952315975	5.005604422315871
>1k	0.011479393297630808	6.701833110410697
>5k	5.118193210786954E-4	1.2629328173644225
>10k+	4.630746238331054E-4	4.546574328251787
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	91840	0.7349761244798587	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	77306	0.6186635918885014	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	43446	0.34768916271942446	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	37312	0.2986000561475663	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	35259	0.2821703307168482	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	33403	0.2673171546820636	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	31235	0.24996710853798332	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	29262	0.23417760621221284	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	26316	0.21060139037251702	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	22437	0.17955857257136967	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22123	0.1770456968844503	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	22083	0.1767255853319765	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17791	0.1423776157515371	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	13502	0.10805365453753324	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	13291	0.10636506609823392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8817011752255425E-4	0.0	0.0	3.201115524738061E-5	8.002788811845152E-6
2	7.122482042542185E-4	8.002788811845152E-6	0.0	4.8016732871070914E-5	8.002788811845152E-6
3	7.442593595015991E-4	8.002788811845152E-6	0.0	4.8016732871070914E-5	8.002788811845152E-6
4	8.562984028674313E-4	6.402231049476121E-5	0.0	5.6019521682916064E-5	8.002788811845152E-6
5	0.0034011852450341897	7.202509930660637E-5	0.0	3.1210876366196094E-4	8.002788811845152E-6
6	0.00810682506639914	7.202509930660637E-5	0.0	6.962426266305282E-4	8.002788811845152E-6
7	0.009299240599364066	7.202509930660637E-5	0.0	7.362565706897539E-4	8.002788811845152E-6
8	0.010875789995297562	8.002788811845151E-5	0.0	8.242872476200506E-4	8.002788811845152E-6
9	0.01211622226113356	2.5608924197904485E-4	0.0	9.123179245503473E-4	1.6005577623690303E-5
10-11	0.023340133569746385	2.5608924197904485E-4	0.0	0.0016405717064282561	2.000697202961288E-5
12-13	0.04016599704665082	2.5608924197904485E-4	0.0	0.0029490276771649386	3.6012549653303185E-5
14-15	0.06001691469443272	4.161450182159479E-4	0.0	0.004297497591960847	4.8016732871070914E-5
16-17	0.06883198657068015	4.321505958396382E-4	0.0	0.004825681653542626	4.8016732871070914E-5
18-19	0.07373769611234123	4.561589622751736E-4	0.0	0.005197811333293426	5.6019521682916064E-5
20-21	0.08979129046890261	4.921715119284768E-4	0.0	0.006050108341754935	5.6019521682916064E-5
22-23	0.094576958178386	5.641966112350833E-4	0.0	0.0070384527600178114	5.6019521682916064E-5
24-25	0.11626851725289229	6.202161329179993E-4	0.0	0.010123527846984117	5.6019521682916064E-5
26-27	0.17370053116109901	6.642314713831476E-4	0.0	0.016149627822303517	5.6019521682916064E-5
28-29	0.18639295421668545	6.682328657890702E-4	0.0	0.02928220426254141	5.6019521682916064E-5
30-31	0.191634780888444	8.242872476200507E-4	0.0	0.05978483381888921	5.6019521682916064E-5
32-33	0.19638843744268003	8.402928252437409E-4	0.0	0.10961019696143712	5.6019521682916064E-5
34-35	0.1997376045604372	9.443290797977279E-4	0.0	0.17359649490654505	5.6019521682916064E-5
36-37	0.2083686122940122	0.0010523667287576375	0.0	0.2741235265665379	5.6019521682916064E-5
38-39	0.21754380966679268	0.0010723737007872504	0.0	0.4474479266534802	5.6019521682916064E-5
40-41	0.22455825406037494	0.001160404377717547	0.0	0.6640314016628515	5.6019521682916064E-5
42-43	0.24011567551060192	0.0011804113497471598	0.0	0.8640971205645743	5.6019521682916064E-5
44-45	0.2577818318127501	0.0012764448154893018	0.0	1.0763110728826781	6.002091608883864E-5
46-47	0.2640240070859893	0.0013444685203899856	0.0	1.295963617401392	6.402231049476121E-5
48-49	0.26815344611290143	0.001348469914795908	0.0	1.5324420253970104	7.202509930660637E-5
50-51	0.27517189190088964	0.0015525410294979594	0.0	1.8022960641324288	7.202509930660637E-5
52-53	0.2773486504577115	0.0015605438183098046	0.0	2.130406404023674	8.40292825243741E-5
54-55	0.2841150083981266	0.00156854660712165	0.0	2.410267930168305	9.603346574214183E-5
56-57	0.2970835276677216	0.001576549395933495	0.0	2.734076771073184	9.603346574214183E-5
58-59	0.3014170378093358	0.0015965563679631077	0.0	3.087587962653225	9.603346574214183E-5
60-61	0.30505430532431943	0.0016365703120223337	0.0	3.4259898889565035	1.0403625455398697E-4
62-63	0.30822741108821605	0.0016765842560815592	0.0	3.7919054031949053	1.0403625455398697E-4
64-65	0.3106202449429577	0.0018406414267243848	0.0	4.142603614507584	1.0403625455398697E-4
66-67	0.31429352500759467	0.0018486442155362301	0.0	4.556351797474384	1.1203904336583213E-4
68-69	0.3180948496932211	0.0019326734980606042	0.0	5.025715361289102	1.1203904336583213E-4
70-71	0.3224323612292411	0.001976688836525752	0.0	5.5165944228244665	1.160404377717547E-4
72-73	0.3353568651603711	0.0019926944141494428	0.0	5.98823878145056	1.3204601539544499E-4
74-75	0.3487415294481821	0.0020607181190501265	0.0	6.4527886677949535	1.3604740980136757E-4
76-77	0.35266689736039214	0.0021247404295448875	0.0	6.897535653224436	1.3604740980136757E-4
78-79	0.3552958134850833	0.0021527501903863457	0.0	7.348752893408294	1.3604740980136757E-4
80-81	0.3606176680449603	0.0021567515847922686	0.0	7.896247684393058	1.3604740980136757E-4
82-83	0.3625343359653972	0.0021687557680100363	0.0	8.480823397337504	1.4405019861321274E-4
84-85	0.369264681356159	0.002188762740039649	0.0	9.049749656760387	1.4405019861321274E-4
86-87	0.3822251978369422	0.0022247752896929524	0.0	9.606683737148721	1.4405019861321274E-4
88	0.3850221725266821	0.00237682827711801	0.0	10.03723377522599	1.4405019861321274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	7600	0.0	20.994246	1
CATGGGG	24730	0.0	18.802052	4
TACACTC	9585	0.0	18.608921	5
TCTACAC	10880	0.0	18.514187	3
TACGTAC	2420	0.0	18.47869	7
GTATAAG	4230	0.0	18.025562	1
CTACACT	12760	0.0	17.261065	4
GTACTAG	3765	0.0	17.251556	1
ATACGTA	2700	0.0	17.065563	6
TATACGT	2795	0.0	16.669687	5
TCTAACG	1810	0.0	16.641317	2
ATCTACA	10145	0.0	16.561607	2
CTAACGC	1705	0.0	15.73404	3
GTATTAG	3655	0.0	14.808963	1
GTATAAA	8075	0.0	14.804896	1
TAATACT	4560	0.0	14.758546	4
TTATACG	3225	0.0	14.738864	4
ATTGCGT	1975	0.0	14.52182	6
CGTTAAC	920	0.0	14.324661	1
TATACAG	5670	0.0	14.276427	5
>>END_MODULE