##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6334689 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.105567139917994 33.0 31.0 34.0 30.0 34.0 2 32.12728233382886 34.0 31.0 34.0 30.0 34.0 3 32.14866744050102 34.0 31.0 34.0 30.0 34.0 4 35.62984828458035 37.0 35.0 37.0 35.0 37.0 5 35.654396608894295 37.0 35.0 37.0 35.0 37.0 6 35.759662707987715 37.0 36.0 37.0 35.0 37.0 7 35.71277469185938 37.0 36.0 37.0 35.0 37.0 8 35.74647942464105 37.0 36.0 37.0 35.0 37.0 9 37.41593075840029 39.0 38.0 39.0 35.0 39.0 10-11 37.34282874818322 39.0 37.5 39.0 34.5 39.0 12-13 37.33076761937326 39.0 37.5 39.0 35.0 39.0 14-15 38.68549568889649 40.5 38.0 41.0 34.5 41.0 16-17 38.632664129209815 40.0 38.0 41.0 34.0 41.0 18-19 38.599065084331684 40.0 38.0 41.0 34.0 41.0 20-21 38.5886940937432 40.0 38.0 41.0 34.0 41.0 22-23 38.492993736551234 40.0 38.0 41.0 34.0 41.0 24-25 38.41817956651069 40.0 38.0 41.0 34.0 41.0 26-27 38.2942012622877 40.0 38.0 41.0 34.0 41.0 28-29 38.15061220842885 40.0 38.0 41.0 33.5 41.0 30-31 37.94265180184852 40.0 38.0 41.0 33.0 41.0 32-33 37.68545969660073 40.0 37.0 41.0 33.0 41.0 34-35 37.55279406771193 40.0 37.0 41.0 33.0 41.0 36-37 37.41452990036291 40.0 37.0 41.0 32.0 41.0 38-39 37.23015391915846 40.0 37.0 41.0 31.5 41.0 40-41 36.993824558711566 40.0 36.0 41.0 31.0 41.0 42-43 36.76031341080833 40.0 36.0 41.0 31.0 41.0 44-45 36.44836068511019 39.0 35.5 41.0 30.0 41.0 46-47 36.079252667336945 39.0 35.0 41.0 29.5 41.0 48-49 35.73913052400836 39.0 35.0 41.0 28.5 41.0 50-51 34.49375217946769 38.0 34.0 39.5 26.0 40.5 52-53 34.458543584381175 38.0 34.0 39.5 26.0 40.0 54-55 35.30060931168049 39.0 35.0 40.0 28.0 41.0 56-57 35.33330547087631 39.0 35.0 41.0 28.0 41.0 58-59 35.13655911126813 38.0 35.0 41.0 27.5 41.0 60-61 34.78850958586917 37.5 35.0 40.0 27.0 41.0 62-63 34.36230728927656 37.0 34.0 40.0 26.0 41.0 64-65 34.07901982244117 36.5 34.0 39.5 26.0 41.0 66-67 33.7894938488693 36.0 34.0 39.0 26.0 41.0 68-69 33.55341556625747 35.5 34.0 39.0 26.0 41.0 70-71 33.18290329643649 35.0 34.0 38.5 25.0 40.5 72-73 32.80511639955805 35.0 34.0 37.0 24.5 39.5 74-75 32.43248745123873 35.0 33.5 37.0 23.5 39.0 76-77 32.07981615198473 35.0 33.0 36.0 23.0 39.0 78-79 31.78632944727042 35.0 33.0 36.0 22.0 37.0 80-81 31.492033073762578 35.0 33.0 35.5 20.0 37.0 82-83 31.256997541631485 35.0 33.0 35.0 20.0 36.5 84-85 31.02093725516754 35.0 33.0 35.0 18.5 36.0 86-87 30.773855038503072 35.0 33.0 35.0 16.5 36.0 88-89 30.578799527490617 35.0 32.5 35.0 10.5 36.0 90-91 30.34461368190293 35.0 32.0 35.0 4.5 35.0 92-93 30.084405958998147 35.0 32.0 35.0 2.0 35.0 94-95 29.805363846591362 35.0 32.0 35.0 2.0 35.0 96-97 29.53470509759832 35.0 31.0 35.0 2.0 35.0 98-99 29.215861899455522 35.0 31.0 35.0 2.0 35.0 100 28.66551096667887 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4695573619929547 1101 2 0.5542897148107784 1101 3 0.6725208801254468 1101 4 0.47699172934098044 1101 5 0.571576716278031 1101 6 0.5401426908033571 1101 7 0.6157753651782301 1101 8 0.5169528564323542 1101 9 0.6572184150777858 1101 10-11 0.6600966449193422 1101 12-13 0.6710847877510204 1101 14-15 0.8133463563951793 1101 16-17 0.8069379798360217 1101 18-19 0.7899681162439336 1101 20-21 0.834725280779459 1101 22-23 0.7274236770046159 1101 24-25 0.79419790924468 1101 26-27 0.8460716073001322 1101 28-29 0.7932425622615611 1101 30-31 0.78960248955336 1101 32-33 0.8240964685248215 1101 34-35 0.9548595547438907 1101 36-37 0.9775041625682519 1101 38-39 0.9018821765145759 1101 40-41 0.9045049762861339 1101 42-43 0.8445449843322734 1101 44-45 0.9771097869932888 1101 46-47 0.9256776221481928 1101 48-49 1.0517090331876062 1101 50-51 1.0545095720473583 1101 52-53 1.0345304868387686 1101 54-55 1.177887726847075 1101 56-57 1.226492105596833 1101 58-59 1.1534617374880156 1101 60-61 1.069863043669983 1101 62-63 1.1356985926765901 1101 64-65 1.1231712886670167 1101 66-67 1.1342743159155333 1101 68-69 1.0781725156911364 1101 70-71 1.026367370623987 1101 72-73 1.0398055556502115 1101 74-75 0.970299114292942 1101 76-77 0.8198293175218367 1101 78-79 0.8697515109942486 1101 80-81 0.9919540016817159 1101 82-83 0.9897253491722502 1101 84-85 0.938406642523848 1101 86-87 0.9613489753281748 1101 88-89 0.9588513583791034 1101 90-91 0.9372649859513338 1101 92-93 0.9637381037402513 1101 94-95 0.9399743993023613 1101 96-97 0.9557846148246263 1101 98-99 0.8644827884850272 1101 100 0.8712798022351969 1102 1 0.4065481382623588 1102 2 0.5606716425322489 1102 3 0.6681420338415549 1102 4 0.45294593118102 1102 5 0.4491502165029644 1102 6 0.3956288797099887 1102 7 0.47382476324919764 1102 8 0.4731287424191635 1102 9 0.6090301562958373 1102 10-11 0.56107998026188 1102 12-13 0.6356855607566843 1102 14-15 0.8153102950486968 1102 16-17 0.8257568380575506 1102 18-19 0.777189069235348 1102 20-21 0.7677469542431226 1102 22-23 0.8142246689648474 1102 24-25 0.8423034720039837 1102 26-27 0.8825053921660029 1102 28-29 0.9418179731934018 1102 30-31 0.9249190327763088 1102 32-33 0.8963453494944957 1102 34-35 0.9412956305958673 1102 36-37 0.887854016974245 1102 38-39 0.9026521071136173 1102 40-41 1.0043998727041554 1102 42-43 1.0052457761558813 1102 44-45 1.04791994093776 1102 46-47 1.076954742768244 1102 48-49 1.1001994081056452 1102 50-51 1.0409827079687517 1102 52-53 1.0689297045221409 1102 54-55 1.1387567040038533 1102 56-57 1.1355216414307918 1102 58-59 1.1592046393514792 1102 60-61 1.17458714278564 1102 62-63 1.1753305083918235 1102 64-65 1.1587903033126707 1102 66-67 1.062061698683209 1102 68-69 1.0854832116314626 1102 70-71 0.997818743402469 1102 72-73 0.9441696881677117 1102 74-75 0.9709593960915868 1102 76-77 0.9462801120407178 1102 78-79 0.9222565021586391 1102 80-81 0.975444200181574 1102 82-83 0.85236590790824 1102 84-85 0.945835991942598 1102 86-87 0.9196906395200877 1102 88-89 0.8850332744373723 1102 90-91 0.8634774076535159 1102 92-93 0.9211622096186041 1102 94-95 0.8107899192998538 1102 96-97 0.8890088016593225 1102 98-99 1.1013035539831222 1102 100 1.1049379655455098 1103 1 0.40693185116781194 1103 2 0.49983520360885336 1103 3 0.5700785417780594 1103 4 0.4352896164812279 1103 5 0.46051460573691827 1103 6 0.4337533333561794 1103 7 0.4784488718298263 1103 8 0.4770576589133029 1103 9 0.5847975821067379 1103 10-11 0.5822375602020671 1103 12-13 0.6034015717987842 1103 14-15 0.7566867615773347 1103 16-17 0.8074017655937809 1103 18-19 0.7491251170844393 1103 20-21 0.7726656337922364 1103 22-23 0.8025346528617945 1103 24-25 0.8093372884290986 1103 26-27 0.813651469920778 1103 28-29 0.7994348353972285 1103 30-31 0.8446358489713859 1103 32-33 0.860737804060868 1103 34-35 0.8847385797562097 1103 36-37 0.8603266417729571 1103 38-39 0.9064210282086265 1103 40-41 0.9101273859316592 1103 42-43 0.9566136160454661 1103 44-45 0.9606992139996393 1103 46-47 0.9439016026716232 1103 48-49 0.8777943011353173 1103 50-51 0.8140179046561187 1103 52-53 0.9163736888791689 1103 54-55 1.0253805825407767 1103 56-57 1.0509358351275964 1103 58-59 0.9843231118612579 1103 60-61 0.9518237427396272 1103 62-63 1.0205832114054019 1103 64-65 1.0286615938578691 1103 66-67 0.9410656094262464 1103 68-69 0.9381533289537529 1103 70-71 0.9448396773799246 1103 72-73 0.8654138795640804 1103 74-75 0.8686139854635684 1103 76-77 0.8624330911067375 1103 78-79 0.8317499459129465 1103 80-81 0.8361633168137352 1103 82-83 0.8911834832475485 1103 84-85 0.8925497025476119 1103 86-87 0.9224541545879497 1103 88-89 0.8875997570690721 1103 90-91 0.9598334002921156 1103 92-93 0.9524146825884685 1103 94-95 0.8322034228655291 1103 96-97 0.8419323121263105 1103 98-99 0.8172774440222277 1103 100 0.8568779241376419 1104 1 0.3626490249564043 1104 2 0.39006517293374543 1104 3 0.4549763740134125 1104 4 0.4204419068608658 1104 5 0.49680980452700396 1104 6 0.39592017518712197 1104 7 0.4110997993459975 1104 8 0.4217616654755503 1104 9 0.5045817113694255 1104 10-11 0.5591422964730342 1104 12-13 0.5236293195280552 1104 14-15 0.6733785151852913 1104 16-17 0.6307078245685958 1104 18-19 0.6557050218976102 1104 20-21 0.6235126940231126 1104 22-23 0.5511474885577101 1104 24-25 0.6372670689183622 1104 26-27 0.716016717329623 1104 28-29 0.728380756633122 1104 30-31 0.6636870909287822 1104 32-33 0.6981503629117825 1104 34-35 0.6731634171952194 1104 36-37 0.6706172093906346 1104 38-39 0.6978205138237996 1104 40-41 0.7359424124626486 1104 42-43 0.7897056157378515 1104 44-45 0.8928111311512126 1104 46-47 0.8227169624419517 1104 48-49 0.7894933945534177 1104 50-51 0.6719727037298853 1104 52-53 0.7355435298641879 1104 54-55 0.8695398709466176 1104 56-57 0.7979806686360931 1104 58-59 0.8031707018572405 1104 60-61 0.7536472455123544 1104 62-63 0.7813437319423358 1104 64-65 0.8526945418298126 1104 66-67 0.7763606116905635 1104 68-69 0.7505985538048634 1104 70-71 0.6722366508831144 1104 72-73 0.6748470821650514 1104 74-75 0.6467565777238775 1104 76-77 0.6114949967231027 1104 78-79 0.5675966558152865 1104 80-81 0.6544599169179754 1104 82-83 0.5874690608196893 1104 84-85 0.5640117858916263 1104 86-87 0.508817556363514 1104 88-89 0.5333521004252191 1104 90-91 0.5743228348960301 1104 92-93 0.580178749133804 1104 94-95 0.49853379122317065 1104 96-97 0.5990624036389605 1104 98-99 0.6769921056988508 1104 100 0.7680396306648589 1105 1 0.2715620447036642 1105 2 0.31969358490119504 1105 3 0.3292134826488464 1105 4 0.3064072678252927 1105 5 0.3820694975559604 1105 6 0.30953837985821053 1105 7 0.3714080520342904 1105 8 0.325954188866433 1105 9 0.3735854211587295 1105 10-11 0.3925734918210182 1105 12-13 0.42825278959340096 1105 14-15 0.4955032546202389 1105 16-17 0.483430662415131 1105 18-19 0.4645827795987074 1105 20-21 0.4434091320074316 1105 22-23 0.4573206464025006 1105 24-25 0.44061486789535564 1105 26-27 0.39428049787056096 1105 28-29 0.3540958683978843 1105 30-31 0.3770359363903353 1105 32-33 0.44184141980598923 1105 34-35 0.4082243620344457 1105 36-37 0.45006589045559764 1105 38-39 0.40906525734342125 1105 40-41 0.5413837905842911 1105 42-43 0.44757284481600834 1105 44-45 0.5228292739787577 1105 46-47 0.44874572981763805 1105 48-49 0.5075303444562707 1105 50-51 0.4430402207727795 1105 52-53 0.43568910132665195 1105 54-55 0.41449347440838835 1105 56-57 0.45437397407517466 1105 58-59 0.47401083192633564 1105 60-61 0.4489624717651495 1105 62-63 0.5194176739002359 1105 64-65 0.4146535804536313 1105 66-67 0.3429757136005094 1105 68-69 0.3144218508449157 1105 70-71 0.3311986778898657 1105 72-73 0.293661663736394 1105 74-75 0.302450803413592 1105 76-77 0.2899126083598418 1105 78-79 0.29279110567109257 1105 80-81 0.2277235509022688 1105 82-83 0.27981489093556533 1105 84-85 0.2562332944953525 1105 86-87 0.26067508171453824 1105 88-89 0.27587196998279495 1105 90-91 0.3519096976136957 1105 92-93 0.3891108288695193 1105 94-95 0.43293662507042896 1105 96-97 0.4407604610299316 1105 98-99 0.4346083004851593 1105 100 0.5046794831090118 1106 1 0.2154770071612404 1106 2 0.3412670591096756 1106 3 0.34184208857608667 1106 4 0.31483641451078626 1106 5 0.36827815253494833 1106 6 0.3376219704661949 1106 7 0.3272633217477292 1106 8 0.4044993666308727 1106 9 0.3749585016469723 1106 10-11 0.43341325806742503 1106 12-13 0.4579532959367114 1106 14-15 0.48152748407323287 1106 16-17 0.4896579232292524 1106 18-19 0.4901234750483141 1106 20-21 0.473621298816866 1106 22-23 0.5164722554890488 1106 24-25 0.5227822802189195 1106 26-27 0.45252831721141007 1106 28-29 0.4717566744776889 1106 30-31 0.48839939576941305 1106 32-33 0.48987080898697144 1106 34-35 0.4133953942061268 1106 36-37 0.523508907782805 1106 38-39 0.5443323582578898 1106 40-41 0.5622291921714009 1106 42-43 0.4568402170306811 1106 44-45 0.48901120086334515 1106 46-47 0.49883427919051115 1106 48-49 0.512917453844679 1106 50-51 0.4770753759204851 1106 52-53 0.46225546227818626 1106 54-55 0.4520012721959503 1106 56-57 0.5700721241259572 1106 58-59 0.536490599545985 1106 60-61 0.5805265342010415 1106 62-63 0.5973122754447999 1106 64-65 0.4487831045311097 1106 66-67 0.4448258721520233 1106 68-69 0.4736968429110604 1106 70-71 0.41528302713361853 1106 72-73 0.4559032063292676 1106 74-75 0.49295881304978195 1106 76-77 0.46378941294582177 1106 78-79 0.3791292562577766 1106 80-81 0.3935327749418107 1106 82-83 0.3747138609158611 1106 84-85 0.41710799483004024 1106 86-87 0.46796154375050847 1106 88-89 0.38420402636299755 1106 90-91 0.43678906531674855 1106 92-93 0.4491733032781262 1106 94-95 0.3808339647374517 1106 96-97 0.4482344467732702 1106 98-99 0.4624794144655766 1106 100 0.5211209454841104 1107 1 0.12856401668799577 1107 2 0.26247819406371065 1107 3 0.2899936486942849 1107 4 0.26949470366727724 1107 5 0.31653636225587434 1107 6 0.27301915749098526 1107 7 0.3269091715152115 1107 8 0.29135425145849325 1107 9 0.3433288037779363 1107 10-11 0.37199770524552633 1107 12-13 0.37894543144791015 1107 14-15 0.3969270431322869 1107 16-17 0.41575765531808884 1107 18-19 0.4739801269495274 1107 20-21 0.43326628435481496 1107 22-23 0.435203879118923 1107 24-25 0.4399641747939498 1107 26-27 0.43539689731905895 1107 28-29 0.4713992144299155 1107 30-31 0.5100058697680581 1107 32-33 0.5132608137126482 1107 34-35 0.42208641743551567 1107 36-37 0.5378702881228747 1107 38-39 0.543593533941042 1107 40-41 0.46998500871201543 1107 42-43 0.46364161156618167 1107 44-45 0.5074498139036479 1107 46-47 0.4454063219165434 1107 48-49 0.47085034550156024 1107 50-51 0.3578340407410181 1107 52-53 0.44952913067515254 1107 54-55 0.5146331821248467 1107 56-57 0.5414023204899436 1107 58-59 0.494603600316438 1107 60-61 0.548166194613998 1107 62-63 0.6062668886836988 1107 64-65 0.5812594715307782 1107 66-67 0.564660767534285 1107 68-69 0.5713097305961625 1107 70-71 0.5402617431827252 1107 72-73 0.4594274240636267 1107 74-75 0.46747507435601676 1107 76-77 0.5272889541686467 1107 78-79 0.5130099043196026 1107 80-81 0.5239330603214363 1107 82-83 0.5038849176847116 1107 84-85 0.5254992039607167 1107 86-87 0.4647134197721847 1107 88-89 0.48965777445296155 1107 90-91 0.5464767106957851 1107 92-93 0.5647269526252643 1107 94-95 0.534886833519824 1107 96-97 0.6047833759560959 1107 98-99 0.5925787824747424 1107 100 0.6343486221592904 1108 1 0.1281828164187786 1108 2 0.1414623282704568 1108 3 0.1613335011709509 1108 4 0.27254288945863436 1108 5 0.3444508769835366 1108 6 0.26740939335707026 1108 7 0.338074132558539 1108 8 0.23590219803369905 1108 9 0.35274219667204676 1108 10-11 0.3405738669521696 1108 12-13 0.36996678726055165 1108 14-15 0.4005174099597113 1108 16-17 0.41725078702901186 1108 18-19 0.4033440848206524 1108 20-21 0.4217218624346728 1108 22-23 0.4582687317682854 1108 24-25 0.4828874592896142 1108 26-27 0.41386058544023996 1108 28-29 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-0.698312877759335 2316 92-93 -0.6108773261690565 2316 94-95 -0.5532485475778195 2316 96-97 -0.5615202740805856 2316 98-99 -0.6366208865743772 2316 100 -0.7788961590588563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17864.0 3 3262.0 4 3224.0 5 3532.0 6 4393.0 7 5069.0 8 6310.0 9 7691.0 10 9844.0 11 13591.0 12 19755.0 13 26410.0 14 26311.0 15 30760.0 16 38551.0 17 49941.0 18 48211.0 19 31413.0 20 28659.0 21 29428.0 22 31467.0 23 36835.0 24 41964.0 25 46229.0 26 51702.0 27 60085.0 28 71615.0 29 85151.0 30 103082.0 31 128836.0 32 165654.0 33 221743.0 34 320286.0 35 510398.0 36 910716.0 37 1579114.0 38 1325993.0 39 239395.0 40 205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57768602799843 17.77054041863313 13.074801884010284 24.576971669358155 2 15.108353315433682 21.898279991925826 36.41694613087702 26.576420561763463 3 19.206396919114557 26.658995299095395 29.14939565796581 24.985212123824237 4 13.61631154688204 17.966814782232408 34.358189737608384 34.058683933277166 5 14.037418845717086 36.027260745678895 33.29463113474445 16.640689273859564 6 31.503428137936133 33.574063602933215 18.243450986392887 16.67905727273776 7 25.99548251367269 31.627890284558635 22.193451937243395 20.183175264525275 8 27.37599158366984 30.968532332882546 21.544417447952725 20.111058635494885 9 27.729140625173017 14.757969527537307 19.646936666138323 37.865953181151355 10-11 25.092831923361658 25.38304933529907 26.184399934194293 23.339718807144983 12-13 27.040682537712502 23.328573712384895 26.326589099953857 23.304154649948742 14-15 25.754456830842987 22.914574691564184 24.743147023542498 26.587821454050324 16-17 23.71505997971446 25.88710518139658 26.87685721265778 23.520977626231183 18-19 24.833360316103146 25.12143036927607 28.060960717303207 21.984248597317578 20-21 24.71952782618828 24.735077334098136 27.303315325838472 23.24207951387511 22-23 23.496667007668755 25.645154073122338 25.36005630171063 25.498122617498275 24-25 23.593290137879716 26.434940798055074 24.88311301395939 25.08865605010582 26-27 24.414034596703196 24.849499715970072 25.342039631365914 25.39442605596081 28-29 23.81121737527069 24.20056505305319 27.704646712169893 24.283570859506227 30-31 25.300797969808 24.406822456364942 26.738328567325453 23.554051006501602 32-33 22.46699941395746 25.43683265879249 27.048595969745364 25.047571957504676 34-35 22.539604083250097 25.80786070376805 27.472804176636085 24.17973103634577 36-37 24.10819337033728 25.855856830923923 26.084109019729844 23.95184077900895 38-39 24.742671332964786 24.396885813203458 25.945171049760663 24.915271804071093 40-41 23.712264871972412 25.59802098066996 25.946632361137063 24.743081786220568 42-43 25.61747293915286 24.859731304642626 25.626762047483776 23.896033708720736 44-45 25.196008820584563 24.471220397605762 26.301434020496302 24.031336761313373 46-47 23.95586585607997 24.77110558578885 27.68552952246292 23.587499035668262 48-49 24.45786489256021 25.601701266457226 26.967134826616597 22.973299014365967 50-51 23.3153695932255 26.059452399541456 25.857087530455942 24.7680904767771 52-53 22.957759922829197 26.353657569113476 24.672152106377876 26.016430401679454 54-55 23.866846250404954 25.72063977259606 24.525706068469802 25.886807908529182 56-57 24.365189573459716 24.319486571879935 25.007725118483414 26.307598736176935 58-59 24.15539801096842 24.186317115118786 26.81228382109873 24.846001052814064 60-61 24.199517575230157 24.79979150857199 25.977366767167876 25.023324149029975 62-63 22.22642527253068 25.26715836034899 26.657143439520397 25.84927292759993 64-65 22.344517326542967 25.12353865984554 26.55645437448771 25.97548963912379 66-67 23.882542051555745 24.958178557982933 25.52619800367945 25.63308138678187 68-69 24.327637024917127 23.932371739444548 25.582787785921102 26.15720344971722 70-71 23.11193627219125 24.48339381766158 26.092426312607124 26.312243597540036 72-73 24.332918356749644 23.93297410152458 26.149896055598692 25.584211486127085 74-75 24.472630464974703 23.33650029995766 26.54847507156517 25.64239416350247 76-77 23.294831912381344 24.411345968627842 27.326729964134106 24.967092154856708 78-79 23.47067725614235 24.158508855779473 27.27878258763369 25.09203130044449 80-81 22.735850800588338 24.189335084838394 26.967350304540993 26.10746381003228 82-83 22.79949665477115 24.959815682450554 25.550460738550534 26.690226924227765 84-85 23.03672785577961 24.596285773780263 25.656192245366544 26.710794125073576 86-87 23.144881228232926 23.397137959407896 26.32651026512139 27.13147054723779 88-89 23.287484855851424 22.909320889932427 27.20993890954225 26.593255344673906 90-91 23.764052171351114 23.40857634842767 26.66241839479055 26.16495308543067 92-93 22.64810919830065 23.782003182946244 27.015206891901105 26.554680726852 94-95 22.265278502891725 23.95442616594099 27.301889189353577 26.478406141813704 96-97 23.531412752965796 23.57628800120132 26.31900836631128 26.5732908795216 98-99 23.61202107036457 23.037778127631743 26.29566158807062 27.054539213933072 100 23.44453563269282 23.28274207904875 26.636369067417835 26.63635322084059 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1361.0 1 1714.5 2 1431.5 3 750.0 4 622.0 5 604.0 6 755.5 7 1129.0 8 1555.5 9 1652.5 10 1430.0 11 1271.0 12 1348.5 13 1492.5 14 1705.5 15 1967.5 16 2267.5 17 2629.0 18 3009.5 19 3455.0 20 4046.0 21 4802.0 22 5767.0 23 6997.0 24 8425.5 25 10568.5 26 13668.5 27 17426.5 28 21694.0 29 28027.0 30 35280.0 31 40999.5 32 47821.5 33 55804.0 34 64039.5 35 70935.0 36 77656.5 37 86183.5 38 91281.5 39 95610.5 40 101007.0 41 106123.5 42 114073.0 43 131638.0 44 154390.0 45 173227.0 46 201755.0 47 242371.0 48 344332.0 49 455481.0 50 536313.0 51 486107.0 52 336344.5 53 275111.0 54 245975.5 55 219748.0 56 201512.5 57 183339.5 58 173620.0 59 158618.5 60 135129.0 61 112025.5 62 91754.0 63 72817.0 64 53796.5 65 43100.5 66 33722.0 67 24142.5 68 18518.0 69 15487.0 70 13474.0 71 11594.0 72 9790.5 73 9756.0 74 8005.0 75 6193.0 76 4743.5 77 3143.0 78 2215.0 79 1565.5 80 1088.5 81 810.0 82 639.5 83 472.5 84 319.0 85 198.0 86 137.5 87 98.0 88 75.0 89 48.0 90 33.5 91 40.5 92 36.5 93 24.5 94 22.0 95 25.5 96 24.0 97 13.5 98 9.0 99 5.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11579100410454247 2 0.366347898057821 3 0.3753301859017862 4 0.37296227170741925 5 0.2990044183700258 6 0.13670757948811693 7 0.1498100380302806 8 0.1253573774497848 9 0.20596117662603483 10-11 0.20599274881529303 12-13 0.21796650159147513 14-15 0.1488076210213319 16-17 0.13431598615180634 18-19 0.1525726045903753 20-21 0.15359870074126766 22-23 0.16089187645991776 24-25 0.17289720142535805 26-27 0.16625125558650156 28-29 0.17419955423225986 30-31 0.21643525041245118 32-33 0.21272551817460966 34-35 0.1303615694472136 36-37 0.09036749870435629 38-39 0.0841714565624295 40-41 0.08353211972995044 42-43 0.07411571428368464 44-45 0.09603470667620778 46-47 0.14310094781290764 48-49 0.22078431948277175 50-51 0.1769621207923546 52-53 0.10903455560328218 54-55 0.05922942704843126 56-57 0.07402099771590996 58-59 0.10870304761607082 60-61 0.08543434413275854 62-63 0.09395883523247944 64-65 0.10273590384626617 66-67 0.09939714483220882 68-69 0.0891677555125437 70-71 0.12381823322344633 72-73 0.1489023375891066 74-75 0.1914853278511384 76-77 0.19114592681661247 78-79 0.15260417677963353 80-81 0.08928615122226205 82-83 0.12969855347279086 84-85 0.15754522439854585 86-87 0.17008727658137598 88-89 0.1586739301645274 90-91 0.17891170347905003 92-93 0.09906563684499745 94-95 0.33947996499907096 96-97 0.3414847990169683 98-99 0.38075270940688644 100 0.38167619594268953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6334689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.13387832040093 #Duplication Level Percentage of deduplicated Percentage of total 1 77.51354359410534 28.783784960066004 2 12.077389689908507 8.969606383462544 3 3.813159635946274 4.247922178124798 4 1.84733771550851 2.7439525577752177 5 1.0749515459018433 1.9958559952922963 6 0.6945290637492546 1.5474334645948078 7 0.4574805259349762 1.1891618328815698 8 0.3459263864127848 1.0276470672694673 9 0.27380719322964236 0.915077069697604 >10 1.5955718889681887 11.167080163171143 >50 0.15171642162462157 3.9152123864592854 >100 0.1248572182487144 9.506462324137882 >500 0.017324166858568472 4.444183977019199 >1k 0.010437271307308284 7.3691450503592435 >5k 0.001026616849898662 2.720373075105096 >10k+ 9.410654457404403E-4 9.457101514584068 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 81049 1.2794471835949641 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 71296 1.125485402677227 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 43249 0.6827328066145 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 37601 0.5935729441492708 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 36242 0.5721196415483065 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 32676 0.5158264281008902 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 32571 0.5141688881648333 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 31392 0.4955570825971093 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 27184 0.42912919639780267 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 26806 0.423162052627998 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24445 0.38589108320866267 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 21561 0.34036398629830134 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 16830 0.2656799726079686 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 15028 0.2372334300863073 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 14693 0.23194508838555455 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 13407 0.21164417069251545 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13043 0.2058980322475184 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 12064 0.19044344560561693 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 11604 0.18318184207622507 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 11300 0.17838286930897476 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 11192 0.17667797108903058 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 10142 0.16010257172846212 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 9879 0.15595082884100545 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 9814 0.15492473269011314 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9659 0.15247788802260062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9291 0.14666860519908712 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 8755 0.13820725847788265 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8589 0.13558676676944992 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 7976 0.12590989076180378 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7926 0.12512058603034812 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 7469 0.11790634078484358 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 7431 0.11730646918893729 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 7125 0.11247592423242878 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6986 0.11028165707898209 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 6964 0.10993436299714163 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 6520 0.10292533698181552 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6398 0.10099943343706377 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.262887570329025E-4 0.0 0.0 0.0 0.0 2 2.3679141943669217E-4 0.0 0.0 1.578609462911281E-5 0.0 3 2.3679141943669217E-4 0.0 0.0 3.157218925822562E-5 0.0 4 2.683636086949178E-4 1.578609462911281E-5 0.0 3.157218925822562E-5 0.0 5 0.0011050266240378967 1.578609462911281E-5 0.0 7.893047314556405E-5 0.0 6 0.0023837002889960344 1.578609462911281E-5 0.0 7.893047314556405E-5 0.0 7 0.0027783526547238546 1.578609462911281E-5 0.0 9.471656777467686E-5 0.0 8 0.0029677857902732085 1.578609462911281E-5 0.0 1.1050266240378968E-4 0.0 9 0.00331507987211369 1.578609462911281E-5 0.0 1.578609462911281E-4 0.0 10-11 0.005675101019166056 1.578609462911281E-5 0.0 2.0521923017846654E-4 0.0 12-13 0.011871143161092834 1.578609462911281E-5 0.0 3.31507987211369E-4 1.578609462911281E-5 14-15 0.018501302905320212 1.578609462911281E-5 0.0 4.025454130423767E-4 1.578609462911281E-5 16-17 0.020679783964137784 1.578609462911281E-5 0.0 5.367272173898356E-4 1.578609462911281E-5 18-19 0.021990029818354143 1.578609462911281E-5 0.0 6.551229271081816E-4 1.578609462911281E-5 20-21 0.026054949185350694 1.578609462911281E-5 0.0 0.0013023528069018068 3.157218925822562E-5 22-23 0.027696703026778426 1.578609462911281E-5 0.0 0.0029677857902732085 3.157218925822562E-5 24-25 0.037886627109870744 1.578609462911281E-5 0.0 0.006235507378499559 6.314437851645125E-5 26-27 0.06930884846911979 1.578609462911281E-5 0.0 0.012463121709684563 6.314437851645125E-5 28-29 0.07649152152536612 1.578609462911281E-5 0.0 0.035605536435963946 7.893047314556405E-5 30-31 0.07809381013022107 1.578609462911281E-5 0.0 0.09699765844858366 7.893047314556405E-5 32-33 0.07943562817369565 1.578609462911281E-5 0.0 0.2089605346055663 7.893047314556405E-5 34-35 0.08028018423635319 1.578609462911281E-5 0.0 0.36374319244401737 7.893047314556405E-5 36-37 0.08186668674657904 1.578609462911281E-5 0.0 0.5874558324804896 7.893047314556405E-5 38-39 0.08395834428493648 1.578609462911281E-5 0.0 0.9175904294591257 7.893047314556405E-5 40-41 0.0858921408770028 1.578609462911281E-5 0.0 1.38841859481973 7.893047314556405E-5 42-43 0.0905806109818493 1.578609462911281E-5 0.0 1.7371176390822027 7.893047314556405E-5 44-45 0.09571898478362553 1.578609462911281E-5 0.0 2.1027235906924555 9.471656777467686E-5 46-47 0.09766856747032096 1.578609462911281E-5 0.0 2.5261697930237776 1.1050266240378968E-4 48-49 0.09911299512888477 1.578609462911281E-5 0.0 2.913465838654431 1.1050266240378968E-4 50-51 0.10112572219409666 1.578609462911281E-5 0.0 3.3396035701200173 1.1050266240378968E-4 52-53 0.10189134778360864 1.578609462911281E-5 0.0 3.853867174852625 1.1050266240378968E-4 54-55 0.1043776576876939 1.578609462911281E-5 0.0 4.314047619385892 1.262887570329025E-4 56-57 0.11065263030276624 1.578609462911281E-5 0.0 4.798483398316792 1.262887570329025E-4 58-59 0.11288636269278571 1.578609462911281E-5 0.0 5.303291132366562 1.262887570329025E-4 60-61 0.11436236254060775 1.578609462911281E-5 0.0 5.7560836846134045 1.420748516620153E-4 62-63 0.11544371002270198 1.578609462911281E-5 0.0 6.222728534897293 1.420748516620153E-4 64-65 0.1161461912336975 1.578609462911281E-5 0.0 6.7398652088524 1.420748516620153E-4 66-67 0.1171959665265335 1.578609462911281E-5 0.0 7.28496694944298 1.420748516620153E-4 68-69 0.11852989152269354 1.578609462911281E-5 0.0 7.9290948616419845 1.420748516620153E-4 70-71 0.12035318545235607 1.578609462911281E-5 0.0 8.697664557802284 1.420748516620153E-4 72-73 0.1251284790776627 1.578609462911281E-5 0.0 9.334073069727655 1.578609462911281E-4 74-75 0.1296354090942744 1.578609462911281E-5 0.0 9.955871551073777 1.736470409202409E-4 76-77 0.13112719503672554 1.578609462911281E-5 0.0 10.632368850309778 1.736470409202409E-4 78-79 0.13197964414669766 3.157218925822562E-5 0.0 11.293427348998506 1.736470409202409E-4 80-81 0.13375557979247282 3.157218925822562E-5 0.0 12.038207400552734 1.736470409202409E-4 82-83 0.1346869593755905 3.157218925822562E-5 0.0 12.904682455602792 1.736470409202409E-4 84-85 0.13754424250345992 3.157218925822562E-5 0.0 13.702685325199074 1.736470409202409E-4 86-87 0.14317198523873864 5.525133120189484E-5 0.0 14.496473307529383 1.736470409202409E-4 88 0.14474270165433537 6.314437851645125E-5 0.0 15.124467831017434 1.8943313554935372E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACAC 7285 0.0 27.863008 3 ATCTACA 6615 0.0 27.834656 2 TACACTC 7200 0.0 27.054655 5 CTACACT 9095 0.0 23.037628 4 CACTCTT 8845 0.0 21.641039 7 AGTACTC 2790 0.0 19.225374 5 ACACTCT 10265 0.0 18.874485 6 ACTCTTT 12430 0.0 17.894234 8 CATGGGG 11290 0.0 17.265804 4 CATGGGT 6420 0.0 17.015057 4 AGTACTT 20280 0.0 16.944677 12-13 ATGGGTA 4180 0.0 16.546844 5 GAGTACT 19710 0.0 16.337584 12-13 GTATTAG 3090 0.0 16.158844 1 TAGTACT 1445 0.0 15.314769 4 AAAGTAC 3615 0.0 14.851722 3 CTAGTAC 1625 0.0 14.780771 3 GTATAAG 1340 0.0 14.412588 1 GTACTTT 22605 0.0 14.26814 14-15 TACTTTT 24470 0.0 14.179073 14-15 >>END_MODULE