##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6334689 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5012909078883 34.0 31.0 34.0 31.0 34.0 2 32.63614756778115 34.0 31.0 34.0 31.0 34.0 3 32.68831413823157 34.0 31.0 34.0 31.0 34.0 4 36.18946312912915 37.0 35.0 37.0 35.0 37.0 5 36.128328951902766 37.0 35.0 37.0 35.0 37.0 6 36.18434433008471 37.0 37.0 37.0 35.0 37.0 7 36.135714476275 37.0 36.0 37.0 35.0 37.0 8 36.1457806373762 37.0 36.0 37.0 35.0 37.0 9 37.881064405845336 39.0 38.0 39.0 35.0 39.0 10-11 37.8320822064035 39.0 38.0 39.0 35.0 39.0 12-13 37.800245994712604 39.0 38.0 39.0 35.0 39.0 14-15 39.17108266877821 40.5 39.0 41.0 36.0 41.0 16-17 39.1414423817807 40.0 38.5 41.0 36.0 41.0 18-19 39.16100072158238 40.0 39.0 41.0 36.0 41.0 20-21 39.14236681548218 40.0 39.0 41.0 36.0 41.0 22-23 39.07689083078901 40.0 39.0 41.0 35.5 41.0 24-25 39.01265539634227 40.0 39.0 41.0 35.0 41.0 26-27 38.88413085472705 40.0 38.0 41.0 35.0 41.0 28-29 38.74839609332045 40.0 38.0 41.0 35.0 41.0 30-31 38.58609475540157 40.0 38.0 41.0 34.5 41.0 32-33 38.34219517327528 40.0 38.0 41.0 34.0 41.0 34-35 38.2689156484241 40.0 38.0 41.0 34.0 41.0 36-37 38.16580640343985 40.0 38.0 41.0 34.0 41.0 38-39 38.00900667736017 40.0 38.0 41.0 33.0 41.0 40-41 37.80395872630842 40.0 37.0 41.0 33.0 41.0 42-43 37.572925442748655 40.0 37.0 41.0 33.0 41.0 44-45 37.27017269513941 40.0 36.5 41.0 32.5 41.0 46-47 36.97002678426676 40.0 36.0 41.0 32.0 41.0 48-49 36.71792916747768 39.0 35.5 41.0 31.0 41.0 50-51 36.466873038281754 39.0 35.0 41.0 31.0 41.0 52-53 36.33899343756261 39.0 35.0 41.0 31.0 41.0 54-55 36.32075244735772 39.0 35.0 41.0 31.0 41.0 56-57 36.14462454273604 39.0 35.0 41.0 31.0 41.0 58-59 36.01078340231068 39.0 35.0 41.0 31.0 41.0 60-61 35.68641522890863 38.0 35.0 40.5 30.0 41.0 62-63 35.34910893020952 37.0 35.0 40.0 30.0 41.0 64-65 35.12561942662063 37.0 35.0 40.0 30.0 41.0 66-67 34.77567920066794 36.0 35.0 39.0 30.0 41.0 68-69 34.42851606448241 36.0 35.0 39.0 29.0 41.0 70-71 34.04768702930799 35.0 34.0 38.5 29.0 40.5 72-73 33.66849588038181 35.0 34.0 37.0 29.0 39.5 74-75 33.323358257998144 35.0 34.0 37.0 29.0 39.0 76-77 31.889512886899418 34.5 32.0 35.5 25.5 37.0 78-79 32.66593577048534 35.0 33.5 36.0 27.5 37.0 80-81 32.62113175563946 35.0 34.0 35.5 29.0 37.0 82-83 32.465414245277074 35.0 34.0 35.0 28.0 36.5 84-85 32.28534376352178 35.0 34.0 35.0 27.5 36.0 86-87 32.12058263633779 35.0 34.0 35.0 27.0 36.0 88-89 31.981894533417503 35.0 34.0 35.0 27.0 36.0 90-91 31.85839778401118 35.0 33.0 35.0 27.0 35.0 92-93 31.71961551703643 35.0 33.0 35.0 26.0 35.0 94-95 31.627815982757795 35.0 33.0 35.0 25.0 35.0 96-97 31.520896684904343 35.0 33.0 35.0 25.0 35.0 98-99 31.390900326756373 35.0 33.0 35.0 25.0 35.0 100 31.06420551979742 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4439288385079152 1101 2 0.3472630786653639 1101 3 0.3900014966336869 1101 4 0.27670852893960074 1101 5 0.33494338666820767 1101 6 0.3229623863954103 1101 7 0.24990471888526855 1101 8 0.30749533115183425 1101 9 0.40284734305151204 1101 10-11 0.4183464867441913 1101 12-13 0.4563992524135827 1101 14-15 0.5459894809263872 1101 16-17 0.5125662062550518 1101 18-19 0.5212374722339206 1101 20-21 0.5329919147939393 1101 22-23 0.5363096762435191 1101 24-25 0.5475257292048994 1101 26-27 0.5612319553883012 1101 28-29 0.5385296599380283 1101 30-31 0.6446009380773603 1101 32-33 0.667874520423787 1101 34-35 0.6231849894951651 1101 36-37 0.6391197618786819 1101 38-39 0.664205708804694 1101 40-41 0.6227720461934041 1101 42-43 0.7212688019454703 1101 44-45 0.7228696518890487 1101 46-47 0.8329027008656524 1101 48-49 0.7901541047570504 1101 50-51 0.834176008184528 1101 52-53 0.705451624317007 1101 54-55 0.7811758771971284 1101 56-57 0.8906610676250253 1101 58-59 0.9194770370262617 1101 60-61 0.9430987083708402 1101 62-63 0.9195954806370068 1101 64-65 0.8350458462617993 1101 66-67 0.8865639753755019 1101 68-69 0.8971965497390642 1101 70-71 0.931696325612883 1101 72-73 0.9061487897074585 1101 74-75 0.8914326824301426 1101 76-77 0.943508403505458 1101 78-79 0.9538057894610219 1101 80-81 0.9506297804957526 1101 82-83 0.9477811464178316 1101 84-85 0.9133539230406171 1101 86-87 1.0132295190382763 1101 88-89 0.9742900619118728 1101 90-91 0.8770207401661736 1101 92-93 1.0099409898599703 1101 94-95 1.0253121634512858 1101 96-97 1.0807760526582548 1101 98-99 1.08725272353038 1101 100 1.1523520313373616 1102 1 0.3441529631061968 1102 2 0.32672530704262215 1102 3 0.3703159228384507 1102 4 0.23213451514173755 1102 5 0.2399899757696744 1102 6 0.24844542917779933 1102 7 0.29706852708734033 1102 8 0.2766423639450366 1102 9 0.30772339457737985 1102 10-11 0.35714138394629913 1102 12-13 0.3618379431750469 1102 14-15 0.4405345939957428 1102 16-17 0.47033579773914624 1102 18-19 0.4360926672103034 1102 20-21 0.4219374657724231 1102 22-23 0.4768451103253497 1102 24-25 0.4267612969725221 1102 26-27 0.42707830201278796 1102 28-29 0.5398007896906662 1102 30-31 0.5570299542617718 1102 32-33 0.5695325940548557 1102 34-35 0.5904273184895956 1102 36-37 0.5705123236961 1102 38-39 0.5576233994318969 1102 40-41 0.5733080634353342 1102 42-43 0.5438822804905783 1102 44-45 0.5350385615168776 1102 46-47 0.604658247992333 1102 48-49 0.6412980786550051 1102 50-51 0.7022368317830114 1102 52-53 0.6225231952130983 1102 54-55 0.7645766414798985 1102 56-57 0.8470454701015413 1102 58-59 0.7883806249800429 1102 60-61 0.7547109206254348 1102 62-63 0.708249868108183 1102 64-65 0.7604594856414764 1102 66-67 0.7865354789386529 1102 68-69 0.7405611556453451 1102 70-71 0.7184365145567568 1102 72-73 0.7232577759595813 1102 74-75 0.7282856197438647 1102 76-77 0.7145261183411513 1102 78-79 0.7367678727833322 1102 80-81 0.6967580752278053 1102 82-83 0.6733886987821833 1102 84-85 0.7191194652373909 1102 86-87 0.7358702443914353 1102 88-89 0.7256220191833904 1102 90-91 0.7940324023880834 1102 92-93 0.8078003634970159 1102 94-95 0.8387703347004098 1102 96-97 0.8266904403450503 1102 98-99 0.8378648578669434 1102 100 0.8584884682585496 1103 1 0.3583689741482914 1103 2 0.28264203122021314 1103 3 0.31928532702524137 1103 4 0.259602654088134 1103 5 0.2930099895722975 1103 6 0.2714836164333789 1103 7 0.29902044795230864 1103 8 0.255030907768365 1103 9 0.30203257332134115 1103 10-11 0.34120855670911965 1103 12-13 0.35168829974279703 1103 14-15 0.4526812468597896 1103 16-17 0.4532376840975516 1103 18-19 0.4054582066628001 1103 20-21 0.43927206434614874 1103 22-23 0.44628863459497126 1103 24-25 0.48029534344134817 1103 26-27 0.5118859854726452 1103 28-29 0.5105692506982535 1103 30-31 0.5580788385497826 1103 32-33 0.5595198266290424 1103 34-35 0.5465814477741517 1103 36-37 0.6111773800568088 1103 38-39 0.5296841309708853 1103 40-41 0.5617483670930312 1103 42-43 0.5622443692874484 1103 44-45 0.5842396197179269 1103 46-47 0.6083858762417051 1103 48-49 0.6164648603435268 1103 50-51 0.6863342552934739 1103 52-53 0.643836054656397 1103 54-55 0.6815823925726008 1103 56-57 0.6252177728390649 1103 58-59 0.5536864680902269 1103 60-61 0.6763085741796075 1103 62-63 0.7344781864886798 1103 64-65 0.6865210223334444 1103 66-67 0.7238497186441961 1103 68-69 0.6839640772004429 1103 70-71 0.6172377567927825 1103 72-73 0.6787645622489791 1103 74-75 0.6255636275653487 1103 76-77 0.5796184808900371 1103 78-79 0.6543237734044496 1103 80-81 0.6327747533966601 1103 82-83 0.66960620510838 1103 84-85 0.6926451151108708 1103 86-87 0.6724964233654305 1103 88-89 0.6719242966160976 1103 90-91 0.7374660347782367 1103 92-93 0.7683716770465878 1103 94-95 0.7789142270399587 1103 96-97 0.8610425222406128 1103 98-99 0.7609142022423789 1103 100 0.8389041572394582 1104 1 0.2818417724188649 1104 2 0.2023639899839793 1104 3 0.25080993974131616 1104 4 0.16930542168360319 1104 5 0.23423261908705229 1104 6 0.22381237840649248 1104 7 0.2549128885459879 1104 8 0.21655440968662276 1104 9 0.25514436230393756 1104 10-11 0.2622384371701898 1104 12-13 0.3199345379832934 1104 14-15 0.39935059653039673 1104 16-17 0.36393921272622975 1104 18-19 0.28723558569264895 1104 20-21 0.35517848390302476 1104 22-23 0.3479911971200593 1104 24-25 0.38574637007486245 1104 26-27 0.41129832216400786 1104 28-29 0.4305610307944647 1104 30-31 0.4762898730443297 1104 32-33 0.4658640372363081 1104 34-35 0.48920508384261296 1104 36-37 0.4996879369082947 1104 38-39 0.5292943092966524 1104 40-41 0.5574333448343509 1104 42-43 0.5013001911496531 1104 44-45 0.551481098617046 1104 46-47 0.548484344195181 1104 48-49 0.6071666282209591 1104 50-51 0.5647559312310335 1104 52-53 0.6089881228764753 1104 54-55 0.6128668508023338 1104 56-57 0.5592790205213376 1104 58-59 0.6606912616100047 1104 60-61 0.7050582430764152 1104 62-63 0.7167748207713132 1104 64-65 0.6574956363312126 1104 66-67 0.664532517512896 1104 68-69 0.6397693628890977 1104 70-71 0.6055094750261034 1104 72-73 0.6126363590926047 1104 74-75 0.6199248761020897 1104 76-77 0.49562280445689666 1104 78-79 0.6152348668567242 1104 80-81 0.5902010519620191 1104 82-83 0.5867156528709856 1104 84-85 0.6038434608765968 1104 86-87 0.5597885033770496 1104 88-89 0.6196679757493371 1104 90-91 0.6279893572599278 1104 92-93 0.6461328726068203 1104 94-95 0.6259671779170581 1104 96-97 0.6155017431441188 1104 98-99 0.5937881608694333 1104 100 0.6490842944657089 1105 1 0.1879589306816456 1105 2 0.13873440391635938 1105 3 0.185190477458697 1105 4 0.1672131540759949 1105 5 0.14777204003246425 1105 6 0.17403683272244308 1105 7 0.14471919251897702 1105 8 0.16554449413100514 1105 9 0.19323298127646638 1105 10-11 0.20340787192731113 1105 12-13 0.23802262868090196 1105 14-15 0.23586956185942398 1105 16-17 0.2528594514174358 1105 18-19 0.181839644704624 1105 20-21 0.27473822592551755 1105 22-23 0.31141070545288585 1105 24-25 0.3180372632587378 1105 26-27 0.3041462025630537 1105 28-29 0.32757189754995863 1105 30-31 0.3052497183244327 1105 32-33 0.28087370341688 1105 34-35 0.31980948983238733 1105 36-37 0.33202660664188244 1105 38-39 0.30342313682309197 1105 40-41 0.235105012147784 1105 42-43 0.2994081098474197 1105 44-45 0.22758252057026596 1105 46-47 0.17290368785385368 1105 48-49 0.19719626560323178 1105 50-51 0.19774408021272905 1105 52-53 0.24184955602839153 1105 54-55 0.3253792406336018 1105 56-57 0.3608101576767311 1105 58-59 0.3076978811205109 1105 60-61 0.3444958450083888 1105 62-63 0.2922027196471717 1105 64-65 0.35444564776750553 1105 66-67 0.32757498029399557 1105 68-69 0.29150930436831146 1105 70-71 0.27342454196070065 1105 72-73 0.3019749262517948 1105 74-75 0.31311516832443687 1105 76-77 0.284148191193335 1105 78-79 0.29631537147152187 1105 80-81 0.27616864863961155 1105 82-83 0.285824606798478 1105 84-85 0.30114981387972506 1105 86-87 0.30745618636293415 1105 88-89 0.31154941499593747 1105 90-91 0.3649328762828148 1105 92-93 0.3357348299569196 1105 94-95 0.35956394414250425 1105 96-97 0.3551121660083716 1105 98-99 0.3554448353013093 1105 100 0.3523322915732514 1106 1 0.1820405669233338 1106 2 0.07190311496405855 1106 3 0.1271014189792652 1106 4 0.09673237494199327 1106 5 0.16474069564783633 1106 6 0.13128000395390416 1106 7 0.17583489787020312 1106 8 0.15049888806392886 1106 9 0.1814020314494229 1106 10-11 0.18877834324442944 1106 12-13 0.19064724883967443 1106 14-15 0.244293726334341 1106 16-17 0.2655299665277724 1106 18-19 0.2249918684033645 1106 20-21 0.26086155531494626 1106 22-23 0.24791096628626264 1106 24-25 0.27816069647911235 1106 26-27 0.26632276708183866 1106 28-29 0.27518994035342104 1106 30-31 0.19472251671070495 1106 32-33 0.24077762630063404 1106 34-35 0.2666241407133967 1106 36-37 0.2272496296463018 1106 38-39 0.1698220620053732 1106 40-41 0.23851355592718448 1106 42-43 0.2641491311922124 1106 44-45 0.20493912710708884 1106 46-47 0.17589326540426242 1106 48-49 0.2818178981103543 1106 50-51 0.24807809765800926 1106 52-53 0.2700528034248677 1106 54-55 0.21840012656274865 1106 56-57 0.21022598301968998 1106 58-59 0.2005928720311161 1106 60-61 0.17422318830112005 1106 62-63 0.15222202885320968 1106 64-65 0.2198642078835249 1106 66-67 0.16430948875914453 1106 68-69 0.17596736127269708 1106 70-71 0.2155825843789927 1106 72-73 0.1818253175855915 1106 74-75 0.20009564726978368 1106 76-77 0.08582866151401092 1106 78-79 0.160126729069475 1106 80-81 0.16866309494017173 1106 82-83 0.1973730359786643 1106 84-85 0.1719521758662097 1106 86-87 0.18258180924392775 1106 88-89 0.20423628347981548 1106 90-91 0.24833302985212669 1106 92-93 0.30047019921407525 1106 94-95 0.3021556063503006 1106 96-97 0.3082477767562146 1106 98-99 0.27901600848211316 1106 100 0.35528510962040727 1107 1 0.16117769808579396 1107 2 0.11400874587920384 1107 3 0.14384246454990546 1107 4 0.06279169050897337 1107 5 0.13515940566572482 1107 6 0.14063372574921829 1107 7 0.047879830451108774 1107 8 0.08104167937823803 1107 9 0.10411288047220069 1107 10-11 0.16187671647023194 1107 12-13 0.1965313648523761 1107 14-15 0.21466141003367767 1107 16-17 0.22748777741595205 1107 18-19 0.20950193299992748 1107 20-21 0.22789873741179179 1107 22-23 0.19304893961282943 1107 24-25 0.2309832333789572 1107 26-27 0.27187553381157414 1107 28-29 0.26816632849914157 1107 30-31 0.3577254017663378 1107 32-33 0.3324927909547526 1107 34-35 0.35122881086272884 1107 36-37 0.3066445586415725 1107 38-39 0.2622281505944173 1107 40-41 0.2508647751344171 1107 42-43 0.2870254709778379 1107 44-45 0.2488080621004869 1107 46-47 0.3313904088498205 1107 48-49 0.22792969263701934 1107 50-51 0.24909669330340023 1107 52-53 0.2841027853128253 1107 54-55 0.31856175587447666 1107 56-57 0.32644371002244554 1107 58-59 0.29718734794945334 1107 60-61 0.24947340114062655 1107 62-63 0.24872134758290088 1107 64-65 0.24800729119709075 1107 66-67 0.2759816442027798 1107 68-69 0.238150607355621 1107 70-71 0.30130238645203633 1107 72-73 0.23744520001359604 1107 74-75 0.2297752203771708 1107 76-77 0.20492077918897778 1107 78-79 0.2866774234871059 1107 80-81 0.24024833095758424 1107 82-83 0.1868765085265025 1107 84-85 0.18697220944244464 1107 86-87 0.2657249201392844 1107 88-89 0.2527927443311313 1107 90-91 0.3054848051083212 1107 92-93 0.29190743132591024 1107 94-95 0.31927939527970395 1107 96-97 0.34080394707930495 1107 98-99 0.3744777920680029 1107 100 0.4260577859921888 1108 1 0.12780899545037983 1108 2 0.03470877776972259 1108 3 0.0537245938783073 1108 4 0.06862692959516892 1108 5 0.10216136755126115 1108 6 0.08723793232562116 1108 7 0.11910476872628095 1108 8 0.07757917031662487 1108 9 0.1105370778258461 1108 10-11 0.1512045625672016 1108 12-13 0.15116071797383057 1108 14-15 0.12292284806690645 1108 16-17 0.14395272426087047 1108 18-19 0.1574575853518354 1108 20-21 0.16763828278133275 1108 22-23 0.1810423735556057 1108 24-25 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-0.6367339819232924 2316 62-63 -0.5232055847209551 2316 64-65 -0.6570600532002189 2316 66-67 -0.5675956152364776 2316 68-69 -0.5145413278351327 2316 70-71 -0.5051053603391225 2316 72-73 -0.5304027201812289 2316 74-75 -0.4953716488440634 2316 76-77 -0.518613388294419 2316 78-79 -0.5877625703284508 2316 80-81 -0.6154365219180704 2316 82-83 -0.5812565480388514 2316 84-85 -0.6048010397878691 2316 86-87 -0.5899260588458404 2316 88-89 -0.5587956678064501 2316 90-91 -0.57835518853868 2316 92-93 -0.588572630561508 2316 94-95 -0.6179388107268728 2316 96-97 -0.611985187118993 2316 98-99 -0.5833984688030505 2316 100 -0.6531029582504182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35.0 3 49.0 4 62.0 5 137.0 6 264.0 7 662.0 8 1332.0 9 2470.0 10 4194.0 11 7228.0 12 12338.0 13 16989.0 14 18838.0 15 23204.0 16 30780.0 17 40186.0 18 40016.0 19 25937.0 20 18628.0 21 17451.0 22 18983.0 23 22043.0 24 25284.0 25 27800.0 26 34356.0 27 43545.0 28 55548.0 29 71781.0 30 91151.0 31 115488.0 32 148454.0 33 196623.0 34 282454.0 35 459005.0 36 888885.0 37 1734136.0 38 1582026.0 39 276145.0 40 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08170630002515 18.52187218662195 13.466722675730411 23.929698837622492 2 15.586277007372138 22.664363031649394 35.70983360249091 26.039526358487564 3 19.695074166902334 26.89799798865671 29.39202893504757 24.014898909393388 4 14.21260639334112 18.484142944152435 34.26245264259005 33.040798019916394 5 14.265783213666843 36.82954285522146 32.69765887480822 16.207015056303476 6 31.77911338662403 33.76552503208919 17.857056597411493 16.598304983875295 7 26.134021733347918 32.21975064600645 22.043592037430727 19.602635583214898 8 27.733453055075003 31.195438323807213 21.417578668818628 19.65352995229916 9 28.108641166125125 14.88734490359353 19.551078198156215 37.452935732125134 10-11 25.52589047851479 25.768538730582296 25.649661451147367 23.055909339755544 12-13 27.398089910503725 23.710798514273105 25.989864992327156 22.901246582896018 14-15 26.024649281118545 23.360121111329047 24.57919062050218 26.03603898705023 16-17 23.968462119877472 26.23222716644345 26.712457543157747 23.08685317052133 18-19 25.25588075436695 25.325244854167266 27.750730935646565 21.66814345581922 20-21 25.309418488757647 25.090679956211247 26.825693600137722 22.774207954893384 22-23 23.8698283907911 26.091462290303475 24.85320611670386 25.18550320220157 24-25 24.067568111551633 26.784721164327287 24.459934218886772 24.68777650523431 26-27 24.78343146413204 25.24478237379053 24.998746972507984 24.973039189569445 28-29 24.262664135484048 24.46420889360745 27.366735997916113 23.906390972992387 30-31 25.85175141478397 24.68707258116459 26.27345554591528 23.18772045813616 32-33 22.823279226863093 25.714870483718883 26.89915825901804 24.562692030399983 34-35 22.875288150402966 26.17401383595525 27.04980162465324 23.900896388988542 36-37 24.557089578068954 26.25250505747513 25.636175472936774 23.554229891519142 38-39 25.07996582799274 24.689496259683892 25.633623070256817 24.596914842066553 40-41 24.003908678062505 25.877848285355597 25.69950300905855 24.418740027523345 42-43 26.068421033106087 25.035024471941313 25.484041793672883 23.412512701279713 44-45 25.62406565772171 24.58665565846366 26.166595892130136 23.62268279168449 46-47 24.18733894816805 25.06179228163609 27.50920392029147 23.24166484990439 48-49 24.930922006807755 25.800996497459426 26.745778698633515 22.522302797099307 50-51 23.66239165998771 26.422602256727405 25.543671386744265 24.371334696540618 52-53 23.244154588769476 26.694137368930104 24.33046083416012 25.7312472081403 54-55 24.175821920333288 25.94651872480906 24.26836583922367 25.609293515633986 56-57 24.67266753164768 24.533241056060024 24.726345935244535 26.067745477047765 58-59 24.307744246456384 24.496117616861717 26.494593355036393 24.701544781645506 60-61 24.581319757999893 25.13489934952404 25.621520735253366 24.662260157222697 62-63 22.337355303597516 25.81224762370114 26.3045538344226 25.54584323827875 64-65 22.383844979396862 25.676068863313912 25.997686537698588 25.94239961959064 66-67 24.00959038637645 25.649737153133458 24.85346135248885 25.487211108001244 68-69 24.482243441523988 24.545088876326467 24.93784521774025 26.03482246440929 70-71 23.310673668864755 25.11171247470767 25.304697831894362 26.272916024533217 72-73 24.47804950203473 24.577739641716953 25.40961952228138 25.534591333966937 74-75 24.574561782256776 23.963575747539092 25.774566328894625 25.687296141309506 76-77 23.299370791582643 25.071653796135717 26.491666595628534 25.13730881665311 78-79 23.547419318657468 24.945085294698725 26.353162352171918 25.154333034471886 80-81 22.719102705752405 24.966237490111983 25.901768816117098 26.41289098801851 82-83 22.62348563911582 25.64436161192738 24.56966127159034 27.16249147736646 84-85 22.916628023619474 25.121290494394476 24.639767211915785 27.322314270070265 86-87 23.01355971160513 23.839248842240327 25.30262928579457 27.844562160359978 88-89 22.98975765799841 23.439535421182807 26.243274329074662 27.327432591744117 90-91 23.537952555408527 23.913371727967714 25.700091678272802 26.84858403835096 92-93 22.058189411064447 24.44803300203066 25.96837973678222 27.52539785012267 94-95 21.484400837747845 24.69429418070872 26.324396335492533 27.496908646050905 96-97 22.73352833394452 24.277733953769733 25.403433890356908 27.585303821928843 98-99 22.750651215868686 23.641721006350906 25.467824860857412 28.139802916922996 100 22.630443005163354 24.15894247569398 25.63544218112336 27.57517233801931 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 571.0 1 369.0 2 191.5 3 234.5 4 226.5 5 270.0 6 435.0 7 812.0 8 1209.5 9 1276.0 10 1046.0 11 908.5 12 1002.5 13 1151.5 14 1324.5 15 1556.5 16 1817.5 17 2138.0 18 2551.5 19 2970.5 20 3395.5 21 3941.5 22 4762.0 23 5708.0 24 6815.5 25 8517.0 26 11153.0 27 14384.5 28 18047.5 29 23881.5 30 30838.0 31 36836.5 32 43996.5 33 52463.5 34 61766.0 35 69693.0 36 77749.5 37 87369.0 38 93544.5 39 98712.5 40 103368.5 41 107808.0 42 115322.5 43 132118.5 44 154286.5 45 172247.5 46 199737.0 47 238470.0 48 335307.5 49 438322.0 50 523310.5 51 497838.5 52 369881.0 53 302632.0 54 259446.5 55 229724.0 56 208632.0 57 188625.0 58 180307.5 59 164251.0 60 137097.5 61 112053.0 62 89297.5 63 69785.0 64 51141.0 65 39230.0 66 30587.5 67 22262.0 68 16682.5 69 13563.5 70 11234.0 71 9522.0 72 7967.0 73 7578.5 74 5605.5 75 4430.5 76 3492.5 77 2208.5 78 1504.0 79 775.0 80 479.5 81 387.0 82 297.0 83 204.0 84 136.0 85 79.5 86 41.0 87 16.5 88 12.5 89 9.5 90 6.0 91 6.0 92 2.5 93 2.0 94 1.5 95 1.5 96 1.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0603818119563565 3 0.025384040163613397 4 0.006488084892565365 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.893047314556405E-5 12-13 1.8943313554935372E-4 14-15 3.394010345259254E-4 16-17 3.31507987211369E-4 18-19 0.0 20-21 0.0029283205537004265 22-23 0.004688470104846505 24-25 6.945881636809636E-4 26-27 4.972619808170535E-4 28-29 0.005746138444997063 30-31 0.00241527247825426 32-33 0.0012628875703290248 34-35 0.0016812190780005143 36-37 0.004001774988480098 38-39 0.004175422029400338 40-41 0.001049775292836002 42-43 0.0029914649322168776 44-45 2.9993579795314343E-4 46-47 0.0025257751406580495 48-49 2.3679141943669216E-5 50-51 0.0018627591662353116 52-53 0.006716983264687501 54-55 0.014831035904051484 56-57 0.025289323595838725 58-59 0.02821764414953915 60-61 0.02579447862397033 62-63 0.00823244834908233 64-65 0.0072616035293918925 66-67 1.8943313554935372E-4 68-69 0.001578609462911281 70-71 1.8154008823479732E-4 72-73 9.55058725061325E-4 74-75 0.00533569998464013 76-77 9.94523961634107E-4 78-79 0.001460213753192935 80-81 0.0 82-83 0.0013576041381037015 84-85 7.893047314556406E-6 86-87 6.472298797936252E-4 88-89 7.893047314556405E-5 90-91 5.761924539626176E-4 92-93 7.656255895119713E-4 94-95 0.0013576041381037015 96-97 1.262887570329025E-4 98-99 0.0 100 7.103742583100765E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6334689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.87904059138211 #Duplication Level Percentage of deduplicated Percentage of total 1 76.98704837416713 27.622214336274403 2 12.147312907821984 8.716678657919296 3 4.0218023185964835 4.328952259183136 4 1.865036130032802 2.676628280553644 5 1.070938743278493 1.9212127320486394 6 0.7228388676413743 1.5560859043880129 7 0.5064130901242576 1.2718731071602838 8 0.36775316992511914 1.0555704729082234 9 0.2860457817664434 0.9236743393492665 >10 1.7012701554876684 11.415268069199108 >50 0.16143250364603565 4.018397969187446 >100 0.13116706800203667 9.662183001616059 >500 0.017989919398662776 4.515340694375716 >1k 0.01074090695247361 7.210099112751214 >5k 0.001149232842651112 2.919735057896456 >10k+ 0.0010608303162933342 10.186086005189251 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 83284 1.3147291050910312 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 69914 1.1036690198997932 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 46836 0.7393575280491276 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 38780 0.6121847497169948 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 37569 0.5930677891211391 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 36263 0.5724511495355179 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 35807 0.5652526903846423 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 32014 0.5053760334564175 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 30594 0.4829597790830773 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 27373 0.43211276828270495 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 25343 0.4000669961856059 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 24000 0.37886627109870746 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 17782 0.280708334694884 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 14981 0.236491483638739 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 14886 0.2349918046489733 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 14064 0.22201563486384254 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 13354 0.2108075076771725 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 13093 0.20668733697897404 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 13072 0.20635582899176264 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 11450 0.18075078350334167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10889 0.17189478441640937 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 10407 0.164285886805177 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 10360 0.16354394035760872 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 10176 0.16063929894585197 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9627 0.15197273299446903 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 9527 0.15039412353155776 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 9333 0.14733162117350984 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 9259 0.1461634501709555 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9253 0.14606873360318082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8767 0.13839669161343202 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 7826 0.12354197656743685 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 7429 0.11727489699967908 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 7283 0.1149701271838286 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 7223 0.11402296150608182 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 7188 0.11347044819406288 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 7105 0.11216020233984653 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6973 0.11007643784880362 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6758 0.10668242750354437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6754 0.10661928312502791 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6619 0.1044881603500977 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 6394 0.10093628905854732 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 6339 0.1000680538539461 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002730994370836516 0.0 0.0 4.735828388733843E-5 0.0 2 0.004530609158555377 0.0 0.0 4.735828388733843E-5 0.0 3 0.00472004229410473 0.0 0.0 4.735828388733843E-5 0.0 4 0.005540919214818596 0.0 0.0 6.314437851645125E-5 0.0 5 0.021816382777433904 0.0 0.0 2.841497033240306E-4 0.0 6 0.04669526791291569 0.0 0.0 5.209411227607227E-4 0.0 7 0.05326228327862662 0.0 0.0 6.156576905353996E-4 0.0 8 0.061818346567605764 0.0 0.0 6.63015974422738E-4 0.0 9 0.06857479506886605 0.0 0.0 8.050908260847533E-4 0.0 10-11 0.13343196485257602 0.0 0.0 0.001460213753192935 0.0 12-13 0.2309347783292913 0.0 0.0 0.0029283205537004265 0.0 14-15 0.33510721678680677 0.0 0.0 0.004712149246790174 0.0 16-17 0.3765536082355424 7.893047314556406E-6 0.0 0.005556705309447709 0.0 18-19 0.3954022052227031 1.578609462911281E-5 0.0 0.006140790810724883 0.0 20-21 0.4609460701227795 1.578609462911281E-5 0.0 0.008050908260847534 0.0 22-23 0.4803629665165883 1.578609462911281E-5 0.0 0.01005574227874486 0.0 24-25 0.5720091388859028 2.3679141943669216E-5 0.0 0.017404169328596873 0.0 26-27 0.7703377387587614 3.157218925822562E-5 0.0 0.04058604929144904 7.893047314556406E-6 28-29 0.8204112309223073 3.157218925822562E-5 0.0 0.06735926578242436 1.578609462911281E-5 30-31 0.8394571540923319 3.157218925822562E-5 0.0 0.12936704548557948 1.578609462911281E-5 32-33 0.8559062646958675 3.157218925822562E-5 0.0 0.24158249915662788 1.578609462911281E-5 34-35 0.8688587553390545 6.314437851645125E-5 0.0 0.3964204083262809 1.578609462911281E-5 36-37 0.8890649564643189 6.314437851645125E-5 0.0 0.620330374545617 1.578609462911281E-5 38-39 0.9094684837724473 6.314437851645125E-5 0.0 0.9505675811393424 3.157218925822562E-5 40-41 0.9290116689232889 6.314437851645125E-5 0.0 1.421672003155956 3.157218925822562E-5 42-43 0.9701423384794423 6.314437851645125E-5 0.0 1.7713182130961758 3.157218925822562E-5 44-45 1.0169875742913346 6.314437851645125E-5 0.0 2.1382186244660155 3.9465236572782024E-5 46-47 1.0346364280866827 6.314437851645125E-5 0.0 2.5618779390748307 4.735828388733843E-5 48-49 1.0458840205099256 7.103742583100764E-5 0.0 2.947990027608301 4.735828388733843E-5 50-51 1.066074435540561 7.893047314556405E-5 0.0 3.372304465144224 4.735828388733843E-5 52-53 1.07284667013645 9.471656777467686E-5 0.0 3.8864891394036865 4.735828388733843E-5 54-55 1.0952076731785887 1.0260961508923327E-4 0.0 4.347111594586569 4.735828388733843E-5 56-57 1.1416187913881801 1.1050266240378968E-4 0.0 4.83447569407117 5.525133120189484E-5 58-59 1.1574206721119222 1.1050266240378968E-4 0.0 5.340712069684873 6.314437851645125E-5 60-61 1.1698127563957756 1.1050266240378968E-4 0.0 5.795193734057031 6.314437851645125E-5 62-63 1.1796869585862857 1.1050266240378968E-4 0.0 6.2639065627373345 7.893047314556405E-5 64-65 1.1871143161092834 1.1050266240378968E-4 0.0 6.782306124262769 7.893047314556405E-5 66-67 1.1985197694788172 1.1839570971834608E-4 0.0 7.327936699023425 7.893047314556405E-5 68-69 1.2109907842358165 1.262887570329025E-4 0.0 7.972767092433425 7.893047314556405E-5 70-71 1.22605071851199 1.262887570329025E-4 0.0 8.742031376757406 7.893047314556405E-5 72-73 1.2642688536090723 1.262887570329025E-4 0.0 9.380657834978166 8.682352046012045E-5 74-75 1.3015635021703513 1.262887570329025E-4 0.0 10.00477687223477 9.471656777467686E-5 76-77 1.312724271073134 1.262887570329025E-4 0.0 10.684123561551324 9.471656777467686E-5 78-79 1.3206488905769487 1.262887570329025E-4 0.0 11.346784348844906 9.471656777467686E-5 80-81 1.3363086964490285 1.262887570329025E-4 0.0 12.092969362821126 9.471656777467686E-5 82-83 1.3430493588556596 1.341818043474589E-4 0.0 12.962159626147393 1.0260961508923327E-4 84-85 1.364573698882455 1.578609462911281E-4 0.0 13.765166687741104 1.1050266240378968E-4 86-87 1.4018052030652175 1.657539936056845E-4 0.0 14.564787632036868 1.1050266240378968E-4 88 1.4112137154641688 1.736470409202409E-4 0.0 15.196326133769157 1.1050266240378968E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 1785 0.0 31.599487 3 GATCTAC 2335 0.0 26.790213 1 CGATCTC 1980 0.0 24.919996 5 AGTACTC 2780 0.0 22.98889 5 TACACTC 3150 0.0 22.07878 5 TCTACAC 3495 0.0 21.518393 3 CCGATCT 2520 0.0 21.446285 4 TCTCGCG 760 0.0 21.0234 8 ATCTACA 3640 0.0 19.252907 2 ATCTCGC 2060 0.0 17.56511 7 CTCGCGC 885 0.0 17.522993 9 AAGTACT 3905 0.0 17.329905 4 AAAGTAC 3835 0.0 17.159306 3 TATCACG 360 2.1791493E-9 16.984482 2 CGTTAAC 610 0.0 16.963064 1 CTACACT 5040 0.0 16.131336 4 AGTACTT 18735 0.0 16.053537 12-13 GTATCAT 2180 0.0 15.534147 1 GTATTAG 3275 0.0 15.510431 1 CACTCTT 4545 0.0 14.992064 7 >>END_MODULE