FastQCFastQC Report
Fri 27 May 2016
ERR765239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6849913
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA230780.3369093884842041No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT202300.29533221808802534No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG92030.13435207133287677No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG71660.10461446736622787No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17250.023.1824721
GTACATA18950.020.8573991
CTAGTAC27250.019.6234533
GTATAAG26500.019.4772451
GTACAAG33200.017.9276071
CGTTAAC6750.017.9110071
TAGTACT30150.017.7359564
TAGTACC14300.017.2339154
TATTCGC3802.8194336E-1017.131239
CATGGGG158500.016.6340474
GTGATCG18200.016.3513158
GTACATG561250.016.3132251
CCTAGTA32800.016.1612552
ATTGCGT12150.016.0737466
TACATGG550950.015.9343342
ATAGTAC16950.015.91118053
GTACAAA34950.015.6994791
TGATCGC19050.015.621739
CATGGGT50050.015.6080734
TCTAACG10200.015.4996822
Produced by FastQC (version 0.11.5)