##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765239_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6849913 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.559765649578324 33.0 33.0 33.0 27.0 33.0 2 31.455288702206875 33.0 33.0 33.0 27.0 33.0 3 31.510893787994096 33.0 33.0 33.0 27.0 33.0 4 35.10753056279693 37.0 37.0 37.0 33.0 37.0 5 35.272477037299595 37.0 37.0 37.0 33.0 37.0 6 35.26286465244157 37.0 37.0 37.0 33.0 37.0 7 35.15956859598071 37.0 37.0 37.0 33.0 37.0 8 35.06807268939036 37.0 37.0 37.0 33.0 37.0 9 35.01886476514373 37.0 37.0 37.0 33.0 37.0 10-11 35.01888162667176 37.0 37.0 37.0 33.0 37.0 12-13 34.96765316581393 37.0 37.0 37.0 33.0 37.0 14-15 36.50380917246686 40.0 37.0 40.0 33.0 40.0 16-17 36.553915064322716 40.0 37.0 40.0 33.0 40.0 18-19 36.565132798037 38.5 37.0 40.0 33.0 40.0 20-21 36.57186967192138 38.5 37.0 40.0 33.0 40.0 22-23 36.62045028893068 37.0 37.0 40.0 33.0 40.0 24-25 36.5937875415352 37.0 37.0 40.0 33.0 40.0 26-27 36.608658752308244 37.0 37.0 40.0 33.0 40.0 28-29 36.54149811829727 37.0 37.0 40.0 33.0 40.0 30-31 36.508063679056946 37.0 37.0 40.0 33.0 40.0 32-33 36.38857135849754 37.0 37.0 40.0 33.0 40.0 34-35 36.341543096970724 37.0 37.0 40.0 33.0 40.0 36-37 36.24069334895202 37.0 37.0 40.0 30.0 40.0 38-39 36.042265865274494 37.0 37.0 40.0 27.0 40.0 40-41 35.96443633663668 37.0 37.0 40.0 27.0 40.0 42-43 35.83404453458022 37.0 37.0 40.0 27.0 40.0 44-45 35.70203548570617 37.0 37.0 40.0 27.0 40.0 46-47 35.58431837017492 37.0 35.0 40.0 27.0 40.0 48-49 35.423685089139084 37.0 33.0 40.0 27.0 40.0 50-51 35.26458350638906 37.0 33.0 40.0 27.0 40.0 52-53 35.0900575671545 37.0 33.0 40.0 27.0 40.0 54-55 34.95486782094896 37.0 33.0 40.0 27.0 40.0 56-57 34.76008469012672 37.0 33.0 40.0 27.0 40.0 58-59 34.53547636298447 37.0 33.0 38.5 27.0 40.0 60-61 34.30376955152569 37.0 33.0 37.0 27.0 40.0 62-63 34.116688343341 37.0 33.0 37.0 27.0 40.0 64-65 33.8135990194328 37.0 33.0 37.0 27.0 40.0 66-67 33.58294973965363 37.0 33.0 37.0 27.0 40.0 68-69 33.28533267502813 37.0 33.0 37.0 27.0 40.0 70-71 33.1220600174046 37.0 33.0 37.0 24.5 37.0 72-73 32.83822152193758 37.0 33.0 37.0 22.0 37.0 74-75 32.568421087975864 35.0 33.0 37.0 22.0 37.0 76-77 32.37289378711817 33.0 33.0 37.0 22.0 37.0 78-79 32.13730451759022 33.0 33.0 37.0 22.0 37.0 80-81 31.88572818370102 33.0 33.0 37.0 22.0 37.0 82-83 31.715555146466826 33.0 33.0 37.0 22.0 37.0 84-85 31.53660834816442 33.0 33.0 37.0 22.0 37.0 86-87 31.334261544635677 33.0 30.0 37.0 22.0 37.0 88-89 31.16829629806977 33.0 27.0 37.0 22.0 37.0 90-91 30.975700421304623 33.0 27.0 37.0 18.5 37.0 92-93 30.838007723601745 33.0 27.0 37.0 15.0 37.0 94-95 30.61903625637289 33.0 27.0 37.0 15.0 37.0 96-97 30.54052723005387 33.0 27.0 37.0 15.0 37.0 98-99 29.392888420626655 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2566004928960446 1101 2 0.267734643994622 1101 3 0.2935888245123195 1101 4 0.24491796953812894 1101 5 0.23604510985503424 1101 6 0.21215037891949606 1101 7 0.2363936525996806 1101 8 0.22918951607461224 1101 9 0.21838706822089904 1101 10-11 0.24249043129979952 1101 12-13 0.18249270989381472 1101 14-15 0.24845180711367476 1101 16-17 0.2863459707896965 1101 18-19 0.25520686868865994 1101 20-21 0.30112569993359983 1101 22-23 0.19504746527985617 1101 24-25 0.26115510179533175 1101 26-27 0.3269158319749579 1101 28-29 0.21308128890476752 1101 30-31 0.2724540919630414 1101 32-33 0.339282532407438 1101 34-35 0.2887880860631711 1101 36-37 0.24508810010348014 1101 38-39 0.2327268953140731 1101 40-41 0.17643674400396492 1101 42-43 0.2093588186339872 1101 44-45 0.25638320491930955 1101 46-47 0.2656222876292347 1101 48-49 0.22367778251894066 1101 50-51 0.1289231502907171 1101 52-53 0.11109377405615106 1101 54-55 0.26801413435338617 1101 56-57 0.324401030790213 1101 58-59 0.17465396342657158 1101 60-61 0.23602703866185948 1101 62-63 0.1955282759886856 1101 64-65 0.06584614656642884 1101 66-67 0.18554279204980872 1101 68-69 0.1997620468648975 1101 70-71 0.1868491315039904 1101 72-73 0.2399648484028276 1101 74-75 0.26082344501868704 1101 76-77 0.16929888308766294 1101 78-79 0.22661015058160672 1101 80-81 0.2194669846861963 1101 82-83 0.24344059464215562 1101 84-85 0.18741987992189024 1101 86-87 0.13626050383917843 1101 88-89 0.13835769662321695 1101 90-91 0.18631593389697798 1101 92-93 0.22332659322833237 1101 94-95 0.22375674486001174 1101 96-97 0.14200146292034788 1101 98-99 0.13198754657776135 1102 1 0.16520915561874006 1102 2 0.20379365800500793 1102 3 0.19671377046093852 1102 4 0.2730582789657561 1102 5 0.1968946603212629 1102 6 0.1497144220518578 1102 7 0.11934075217529738 1102 8 0.16245441964198193 1102 9 0.11169214038925901 1102 10-11 0.13462098721394256 1102 12-13 0.15628250015898715 1102 14-15 0.18884117924021382 1102 16-17 0.2549778321270537 1102 18-19 0.2592727162828723 1102 20-21 0.2574182392067357 1102 22-23 0.18485578480753873 1102 24-25 0.26098199418935764 1102 26-27 0.2801847393060015 1102 28-29 0.1732359223448512 1102 30-31 0.1727853713342995 1102 32-33 0.28272465501768096 1102 34-35 0.24289052059661742 1102 36-37 0.3021796376491537 1102 38-39 0.33226488557514244 1102 40-41 0.27674277935124536 1102 42-43 0.23455910036364003 1102 44-45 0.21654378997738633 1102 46-47 0.28278023912260153 1102 48-49 0.22190791355534145 1102 50-51 0.2527886392185792 1102 52-53 0.1978982084134202 1102 54-55 0.23066052936052728 1102 56-57 0.24803366302199947 1102 58-59 0.23803327322368517 1102 60-61 0.2406378339633477 1102 62-63 0.19117077524901305 1102 64-65 0.16201512013178387 1102 66-67 0.24203817983701015 1102 68-69 0.15533523740980115 1102 70-71 0.2304313314335289 1102 72-73 0.2184602064462453 1102 74-75 0.22132985869605193 1102 76-77 0.14101750074529917 1102 78-79 0.15937345732081099 1102 80-81 0.2161823931474096 1102 82-83 0.12815199815605283 1102 84-85 -0.03436541303918261 1102 86-87 0.18778996084505195 1102 88-89 0.24867565162474747 1102 90-91 0.17700657244001405 1102 92-93 0.2078405118434894 1102 94-95 0.2138534959275873 1102 96-97 0.10091027046352252 1102 98-99 0.12837888538234665 1103 1 0.0726367471204199 1103 2 0.11474853917809469 1103 3 0.09985872533392026 1103 4 0.12332928338593518 1103 5 0.19542483008248723 1103 6 0.08690153937559586 1103 7 0.039672935435952184 1103 8 0.05977443562396445 1103 9 0.09658991304687703 1103 10-11 0.05077054748208809 1103 12-13 0.05904788841804276 1103 14-15 0.03783288697108844 1103 16-17 0.002190029146177608 1103 18-19 0.0798610982434198 1103 20-21 0.0845242091794276 1103 22-23 0.14045222647605726 1103 24-25 0.1488415643865082 1103 26-27 0.1965634390602986 1103 28-29 0.11170608055879683 1103 30-31 0.182719451522118 1103 32-33 0.2111260119272984 1103 34-35 0.14478283692982075 1103 36-37 0.14066903011764254 1103 38-39 0.2038341528151193 1103 40-41 0.18203073532550462 1103 42-43 0.09697636263871345 1103 44-45 0.043669154069377214 1103 46-47 0.024016677472666004 1103 48-49 0.11540152449018137 1103 50-51 0.0989526899649178 1103 52-53 0.0758568773715993 1103 54-55 0.0753332522651462 1103 56-57 0.041951526977541675 1103 58-59 0.08176514995909656 1103 60-61 0.053993022772317545 1103 62-63 0.05387645317963319 1103 64-65 0.057038538636007274 1103 66-67 0.14676864252655974 1103 68-69 0.12167042590353105 1103 70-71 0.1038232672605588 1103 72-73 0.09183583326333888 1103 74-75 0.07635964430507158 1103 76-77 0.019751892405764693 1103 78-79 0.005498803967896038 1103 80-81 0.048182734865342525 1103 82-83 0.03969042776471099 1103 84-85 0.018927481241579613 1103 86-87 0.13777579458379563 1103 88-89 0.03649565669666899 1103 90-91 0.04924187272128222 1103 92-93 0.09573917879017202 1103 94-95 0.06247200358839322 1103 96-97 -0.07287889002157044 1103 98-99 0.04764404150094137 1104 1 0.08267687093090714 1104 2 0.1096097566553631 1104 3 0.011064678316071053 1104 4 0.08711789640434375 1104 5 0.15310400103395239 1104 6 0.09281569402001111 1104 7 0.04399363109558152 1104 8 0.058852128157369066 1104 9 0.04615179666656388 1104 10-11 0.049369991971687455 1104 12-13 0.04695149714898861 1104 14-15 0.12048502815758866 1104 16-17 0.10355536392626163 1104 18-19 0.03823183852775003 1104 20-21 0.10730657788040077 1104 22-23 0.0908660770689238 1104 24-25 0.06572031829849578 1104 26-27 0.09841951938173565 1104 28-29 0.0025882866520703374 1104 30-31 0.005875962653846045 1104 32-33 0.13086525119206271 1104 34-35 0.09098256836824703 1104 36-37 0.15643733257099512 1104 38-39 0.08214351456825142 1104 40-41 0.044001736531562585 1104 42-43 -0.020320014113501372 1104 44-45 -0.07231920643003065 1104 46-47 -0.06633132510693684 1104 48-49 -0.04724443133250844 1104 50-51 0.0793251961658541 1104 52-53 0.011375245018918179 1104 54-55 0.1271553346814258 1104 56-57 0.0040779391653131825 1104 58-59 0.09622338876888392 1104 60-61 0.0743595545187361 1104 62-63 0.008337490464079167 1104 64-65 0.027924431715433684 1104 66-67 0.03556182290914478 1104 68-69 -0.029294338277161103 1104 70-71 -0.03915794907914716 1104 72-73 0.03776305138264746 1104 74-75 -0.003691988085179787 1104 76-77 -0.11425000095389493 1104 78-79 -0.06797871279279377 1104 80-81 -0.04156107709241752 1104 82-83 -0.0031714313031194763 1104 84-85 -0.020334924642249064 1104 86-87 -0.021842042262047556 1104 88-89 0.02355670803799015 1104 90-91 0.08160896102143056 1104 92-93 0.13851339270571117 1104 94-95 0.02150472420218108 1104 96-97 -0.24053064613032404 1104 98-99 -0.10785764243588858 1105 1 -0.009876011270733898 1105 2 0.06367582817572526 1105 3 0.06515370253573138 1105 4 -0.013190999107735024 1105 5 0.010254581604534962 1105 6 0.037940068084388656 1105 7 -0.014788853357451615 1105 8 -0.11404136112664531 1105 9 -0.1143078419160517 1105 10-11 -0.011740072017012437 1105 12-13 -0.06908590494366962 1105 14-15 -0.05880072348536203 1105 16-17 -0.033529884605165705 1105 18-19 -8.349045963740309E-5 1105 20-21 0.011626803368969263 1105 22-23 0.03363530764001155 1105 24-25 0.045848664708536546 1105 26-27 0.14008219634402508 1105 28-29 0.05756021145232637 1105 30-31 0.07789858206583489 1105 32-33 0.12433788484380415 1105 34-35 0.08194405408599437 1105 36-37 0.06758080648414477 1105 38-39 -0.004546406055389696 1105 40-41 0.04976683136109017 1105 42-43 0.06326324270150963 1105 44-45 0.01661849964170159 1105 46-47 -0.022798988678935928 1105 48-49 -0.015241052654857867 1105 50-51 0.08000891950469224 1105 52-53 -0.048516447246257144 1105 54-55 0.07490472424266414 1105 56-57 0.1026720737343112 1105 58-59 -0.007868586432117297 1105 60-61 0.05001733218004745 1105 62-63 0.016092691834678874 1105 64-65 -0.028183732894007107 1105 66-67 0.05843031844510449 1105 68-69 -0.06872644851551968 1105 70-71 -0.06189422441208592 1105 72-73 -0.060622526639569685 1105 74-75 0.038576465991440045 1105 76-77 -0.08774650675680817 1105 78-79 -0.013247402163990785 1105 80-81 -0.06324612831686238 1105 82-83 0.016866333978622805 1105 84-85 -0.07343692399650692 1105 86-87 -0.09083844990422563 1105 88-89 0.0054334968954457 1105 90-91 0.020379989843277713 1105 92-93 0.03174790960094853 1105 94-95 0.024109875170914563 1105 96-97 -0.10150722720957361 1105 98-99 -0.002082949917500798 1106 1 0.012966205658255348 1106 2 0.1632453443674713 1106 3 0.0529775183921295 1106 4 0.05643791894840433 1106 5 0.03164192118737219 1106 6 0.02170056333953596 1106 7 0.0532676688126017 1106 8 0.001326066742123544 1106 9 0.061867588540522434 1106 10-11 0.03477254625030923 1106 12-13 0.05183871219530545 1106 14-15 0.09718803873619208 1106 16-17 0.074918407781837 1106 18-19 0.13221303310452726 1106 20-21 0.12039337059687227 1106 22-23 0.09270310461938891 1106 24-25 0.09813975889436932 1106 26-27 0.11943253337935289 1106 28-29 0.05381048237667585 1106 30-31 0.03117978901065044 1106 32-33 0.11338962785395523 1106 34-35 -0.01847935029213943 1106 36-37 -3.424862568195408E-4 1106 38-39 0.048224918549550466 1106 40-41 0.09707623948806088 1106 42-43 0.10789722527303525 1106 44-45 0.10374815014657912 1106 46-47 0.00971430158967479 1106 48-49 -0.02614656077823696 1106 50-51 -0.029913963718485093 1106 52-53 0.018040624396491012 1106 54-55 -0.01363702751331175 1106 56-57 0.025364914687841633 1106 58-59 0.07966633421836633 1106 60-61 0.10790095832950186 1106 62-63 0.10815795764549563 1106 64-65 -0.05258328830579728 1106 66-67 -0.008958313950586216 1106 68-69 -0.017729283554999142 1106 70-71 -0.03217744518247656 1106 72-73 0.09920094504762034 1106 74-75 0.08330738229841472 1106 76-77 0.05413798852884355 1106 78-79 0.0600090975697114 1106 80-81 0.04280343531795339 1106 82-83 0.049457389064212265 1106 84-85 -0.06406324117893547 1106 86-87 -0.08487413894515683 1106 88-89 0.10659939734994239 1106 90-91 0.02240879114327754 1106 92-93 -0.05983260584691408 1106 94-95 -0.08765597431168715 1106 96-97 -0.22898129743533957 1106 98-99 0.2686472824076098 1107 1 0.049874556362905054 1107 2 0.08633583557491065 1107 3 0.07347923770725373 1107 4 -0.02721789732540003 1107 5 0.09521827481396627 1107 6 -0.0029127243829449867 1107 7 -0.04004811503474315 1107 8 -0.03600765855942001 1107 9 -0.08736486642767005 1107 10-11 0.0037729382185318627 1107 12-13 -0.029931425171731973 1107 14-15 0.026803108132376963 1107 16-17 -0.026860370702678438 1107 18-19 -0.039678258074410167 1107 20-21 -0.02785978741976436 1107 22-23 -0.053212185704339277 1107 24-25 0.07936483773342928 1107 26-27 0.06397433144445586 1107 28-29 -0.013924921261505574 1107 30-31 -0.04622212985192675 1107 32-33 -0.09091249055608586 1107 34-35 -0.09727578516306323 1107 36-37 -0.012364062325382008 1107 38-39 -0.012807071401809367 1107 40-41 -0.1755728420591467 1107 42-43 -0.14369857532204122 1107 44-45 -0.014182480387752605 1107 46-47 -0.16948447974550618 1107 48-49 -0.04142735255403096 1107 50-51 -0.05678877441565078 1107 52-53 -0.09352328423928213 1107 54-55 -0.012114854584062584 1107 56-57 -0.006216053473650618 1107 58-59 0.019230714997668485 1107 60-61 0.08539396526020937 1107 62-63 -0.06186716395373537 1107 64-65 -0.13834740361571107 1107 66-67 0.0761166945123648 1107 68-69 0.009939485486640365 1107 70-71 -0.03806721303039495 1107 72-73 -0.008897029189377292 1107 74-75 -0.057637127474066574 1107 76-77 -0.11672848393539681 1107 78-79 0.06265497210425508 1107 80-81 -0.16335532071746073 1107 82-83 -0.04823850595957069 1107 84-85 -0.1753559790769188 1107 86-87 -0.1835531169771194 1107 88-89 -0.060027048032846864 1107 90-91 0.05350438684587999 1107 92-93 0.02646378448216069 1107 94-95 0.06562404243604192 1107 96-97 -0.05106407399564006 1107 98-99 0.28788534964797563 1108 1 0.01998289327845626 1108 2 0.028245293694940443 1108 3 -0.010681084585744571 1108 4 -0.0033065065842166064 1108 5 -0.027282018276558517 1108 6 -0.16369603989400616 1108 7 -0.03832255789630068 1108 8 -0.06592680110088622 1108 9 0.012652292690283673 1108 10-11 -0.056773933173346336 1108 12-13 -0.06587387107433074 1108 14-15 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2215 66-67 -0.31838700784157936 2215 68-69 -0.1642950300599324 2215 70-71 -0.240081144483014 2215 72-73 -0.20120135108710713 2215 74-75 -0.09886507486253038 2215 76-77 -0.1226195750944683 2215 78-79 -0.09813825929700926 2215 80-81 0.07584776209268185 2215 82-83 -0.07597008944061301 2215 84-85 -0.03418982041407048 2215 86-87 -0.013168552562788705 2215 88-89 -0.14486905608360345 2215 90-91 0.05428457304032719 2215 92-93 0.08321735017202414 2215 94-95 0.04197126028685716 2215 96-97 0.11428520624024685 2215 98-99 -0.007526464753933482 2216 1 -0.006117001418562751 2216 2 -0.0207879836302034 2216 3 0.04549315930991327 2216 4 0.05251117068342381 2216 5 -0.06084964723497421 2216 6 0.06070559064445291 2216 7 0.07843495094100916 2216 8 0.05101680032040434 2216 9 0.06465717507680324 2216 10-11 -0.015654608703350448 2216 12-13 0.015801907335145415 2216 14-15 -0.014411003141269418 2216 16-17 -0.020466877781245785 2216 18-19 0.0037830825151772274 2216 20-21 0.005981483995704195 2216 22-23 -0.12898295653715763 2216 24-25 -0.04652568615519925 2216 26-27 -0.1272667738539326 2216 28-29 -0.015370790648859156 2216 30-31 -0.03160847602008232 2216 32-33 -0.0073601574789492474 2216 34-35 -0.1698192359353783 2216 36-37 -0.10334781012495142 2216 38-39 -0.05903035630498721 2216 40-41 -0.06028495744853046 2216 42-43 -0.0659115894791853 2216 44-45 -0.06675602438398442 2216 46-47 0.005261014018905996 2216 48-49 0.04775653532934143 2216 50-51 0.0314890625912696 2216 52-53 0.18739996719254748 2216 54-55 0.11064157315501433 2216 56-57 0.1893793336783034 2216 58-59 0.040306478020504244 2216 60-61 0.08012858083554875 2216 62-63 0.05469759071365843 2216 64-65 0.1861404227299559 2216 66-67 0.051628186342867366 2216 68-69 0.009416557611203302 2216 70-71 0.005910423148414168 2216 72-73 -0.050937882832805315 2216 74-75 0.041070847385235254 2216 76-77 0.06997415410293684 2216 78-79 0.044826642944507 2216 80-81 0.1903268997967551 2216 82-83 0.13609250090825498 2216 84-85 0.16923182743387954 2216 86-87 0.08508720236435963 2216 88-89 0.08351177253591757 2216 90-91 0.1963141518026177 2216 92-93 0.3082307618951994 2216 94-95 0.27594725791716 2216 96-97 0.29491155390211077 2216 98-99 0.3422321314395589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 210.0 8 1188.0 9 2179.0 10 2907.0 11 5062.0 12 9646.0 13 17891.0 14 28072.0 15 32132.0 16 33555.0 17 35291.0 18 36575.0 19 37592.0 20 39653.0 21 43246.0 22 47403.0 23 53126.0 24 59950.0 25 68936.0 26 76832.0 27 87394.0 28 105877.0 29 132089.0 30 167314.0 31 215887.0 32 290182.0 33 392324.0 34 544723.0 35 795162.0 36 1258960.0 37 1670137.0 38 557810.0 39 606.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15401359312738 18.383336119308854 12.435324612378345 25.027325675185423 2 15.961971482261378 20.797814628859467 37.70664499047579 25.533568898403363 3 18.310679974493073 26.1188040007568 28.611495869139187 26.95902015561094 4 13.104239426106579 17.360176691295205 35.992092162338416 33.5434917202598 5 14.579659624874067 37.404548057763655 33.07663907556198 14.939153241800298 6 32.12617444922293 36.22162792432546 17.58863214759078 14.063565478860825 7 28.098838627585486 31.6091897809505 21.95467300095636 18.337298590507643 8 26.915743309440572 33.51800234543125 20.0414370226308 19.52481732249738 9 27.073964297064794 15.667381468932525 19.007380093732575 38.251274140270105 10-11 25.488017147079095 25.740618895451668 26.694704005729708 22.076659951739533 12-13 26.370386018041398 23.51907973137761 26.245121653369903 23.86541259721109 14-15 24.385397303586192 24.508858725650967 23.52784626607666 27.577897704686176 16-17 24.265935932324982 26.888173616219653 24.55142860938526 24.29446184207011 18-19 24.775051887520323 26.34426889801374 25.59770467157758 23.282974542888354 20-21 25.969014796969148 25.748091299376302 24.55838476061451 23.724509143040045 22-23 25.19298420286506 25.586646136965534 24.37183508754053 24.84853457262888 24-25 24.66856079270065 26.119587765528397 24.371514119735803 24.84033732203515 26-27 24.903376474772916 25.92590078366717 24.271134637452562 24.899588104107345 28-29 24.512612414394823 25.893551815266996 24.993886257079716 24.599949513258466 30-31 24.98837752428895 25.878897489289766 24.66178759917426 24.470937387247023 32-33 24.376796329001525 26.159689017698412 24.392672455795935 25.070842197504128 34-35 24.691032436922526 26.240162279504403 24.77771240790025 24.291092875672817 36-37 24.824986828299863 26.127820893491638 24.40044858963902 24.646743688569476 38-39 24.92549173266631 25.834386753346138 24.465011297227075 24.775110216760478 40-41 24.71383213188255 26.112915594694414 24.743124474719604 24.43012779870343 42-43 25.16610794910826 25.95795742223295 24.542355501449435 24.333579127209354 44-45 24.654335025860913 26.18920853447336 24.45382153028805 24.70263490937768 46-47 24.298899854640492 26.192172075762134 25.082734627432497 24.426193442164887 48-49 24.875795550457667 26.19400544139688 24.738829957332925 24.19136905081253 50-51 24.03924692182222 26.465248536733238 24.7557523723294 24.73975216911514 52-53 24.182606406825897 26.23055212526057 24.461091695617156 25.12574977229638 54-55 24.445719237601995 26.057805405703693 24.528340724911395 24.968134631782917 56-57 24.54733366686555 25.665165382392445 24.44684333947015 25.34065761127185 58-59 24.38727323982071 25.655084962392955 25.021076910027908 24.93656488775843 60-61 24.53614374372346 25.91940948739057 24.713518259283003 24.830928509602966 62-63 24.006772056812984 26.09229489484027 24.6643059554187 25.236627092928043 64-65 24.021575164531285 25.84045957955962 24.8262277199725 25.31173753593659 66-67 24.72304926072381 25.864254118977776 24.565550829540157 24.84714579075826 68-69 24.709605800832797 25.513951783037243 24.653269318895 25.123173097234957 70-71 24.08953223201521 25.786035530670244 24.711547431332338 25.412884805982205 72-73 24.766117467477326 25.795079441154943 24.711218960007226 24.7275841313605 74-75 25.06949358334916 25.520302228656043 24.6795543242666 24.730649863728196 76-77 24.62417405885301 25.79364876605002 24.91860115595629 24.66357601914068 78-79 24.84497978295491 25.70823162279579 24.859578508515366 24.587210085733936 80-81 24.450536817036948 25.560149450073304 25.179429286182177 24.80988444670757 82-83 24.657627038474793 25.752487659332317 24.694488820514945 24.895396481677942 84-85 24.73823390165685 25.75676508592153 24.457179237166955 25.047821775254665 86-87 24.599575206283642 25.282116721774422 24.747854461801193 25.370453610140743 88-89 24.72528483208473 24.967769663643903 25.012003802092085 25.294941702179287 90-91 25.01082130532169 25.270058174461486 24.701926871188 25.01719364902883 92-93 24.64934956108202 25.320518669361203 24.775183276050367 25.25494849350641 94-95 24.185131986347855 25.49081280302392 25.1551662043007 25.16888900632753 96-97 24.84792872551812 25.36464331736768 24.67219656658413 25.11523139053007 98-99 24.686430324005578 25.49498073917143 24.506530228924074 25.312058707898917 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 808.0 1 750.0 2 711.0 3 715.5 4 657.5 5 592.5 6 559.0 7 529.5 8 516.0 9 501.5 10 483.0 11 488.5 12 505.5 13 542.0 14 601.0 15 733.5 16 890.5 17 1079.0 18 1554.5 19 2201.5 20 3032.0 21 4238.5 22 5740.5 23 7784.5 24 10526.0 25 14185.0 26 18686.5 27 23880.0 28 30400.5 29 37833.5 30 45153.5 31 52942.0 32 60938.0 33 69556.0 34 77795.0 35 85874.5 36 93559.0 37 102198.0 38 114824.0 39 123401.5 40 132350.5 41 143964.0 42 157377.5 43 177361.5 44 198698.0 45 221166.5 46 252508.5 47 282886.5 48 304636.5 49 330444.5 50 357881.5 51 345215.0 52 318377.0 53 313556.0 54 299925.5 55 285055.0 56 262277.5 57 241885.0 58 228726.5 59 201212.0 60 167331.0 61 130634.5 62 106600.0 63 86216.5 64 63088.0 65 48273.5 66 36080.5 67 27758.5 68 21988.5 69 18491.5 70 16606.0 71 14724.5 72 14225.5 73 12659.5 74 9182.0 75 6800.5 76 5131.5 77 3604.0 78 2286.5 79 1524.5 80 1081.0 81 729.5 82 498.5 83 341.5 84 258.5 85 203.0 86 139.0 87 93.5 88 67.5 89 57.0 90 39.0 91 24.0 92 20.5 93 21.0 94 16.0 95 9.5 96 6.0 97 6.5 98 7.5 99 10.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001868636871738371 2 1.4598725560456024E-5 3 1.313885300441042E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.452611295939087E-4 22-23 0.0 24-25 0.004613197277104104 26-27 3.649681390114006E-5 28-29 0.008613248080669053 30-31 5.620509340775569E-4 32-33 8.029299058250813E-5 34-35 4.379617668136807E-5 36-37 0.0 38-39 2.9197451120912048E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.554776973079804E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.109553946159608E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6849913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.33388222390668 #Duplication Level Percentage of deduplicated Percentage of total 1 72.88151296185265 30.124758730653454 2 14.454320105416574 11.9490632972787 3 5.1368135749387775 6.369733419380533 4 2.325103776473986 3.844222626205455 5 1.2859373261333884 2.6576390992861474 6 0.8121598931010773 2.014183282105234 7 0.5467159950698354 1.5818526185099762 8 0.3846930546318015 1.2720685930004623 9 0.28453355516526757 1.0584788812155561 >10 1.6026145427544942 12.159012689061784 >50 0.13936183322440093 4.0310311206604466 >100 0.12025889274143754 10.291588451370203 >500 0.016819603585056693 4.75749195340155 >1k 0.008871012511131308 6.648140500326264 >5k 2.129043002286975E-4 0.6055390970311041 >10k+ 7.09681000762325E-5 0.6351956405130809 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 23078 0.3369093884842041 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 20230 0.29533221808802534 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9203 0.13435207133287677 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7166 0.10461446736622787 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4598725560456024E-5 0.0 2 0.0 0.0 0.0 2.9197451120912048E-5 0.0 3 0.0 0.0 0.0 2.9197451120912048E-5 0.0 4 0.0 0.0 0.0 2.9197451120912048E-5 0.0 5 0.0 0.0 0.0 2.9197451120912048E-5 0.0 6 0.0 0.0 0.0 2.9197451120912048E-5 0.0 7 0.0 0.0 0.0 2.9197451120912048E-5 0.0 8 1.4598725560456024E-5 0.0 0.0 4.379617668136807E-5 0.0 9 1.4598725560456024E-5 0.0 0.0 4.379617668136807E-5 0.0 10-11 2.9197451120912048E-5 0.0 0.0 8.759235336273614E-5 0.0 12-13 2.9197451120912048E-5 0.0 0.0 8.759235336273614E-5 0.0 14-15 4.3796176681368075E-5 0.0 0.0 1.0219107892319216E-4 2.9197451120912048E-5 16-17 5.8394902241824096E-5 0.0 0.0 1.5328661838478824E-4 2.9197451120912048E-5 18-19 6.569426502205211E-5 0.0 0.0 1.970827950661563E-4 2.9197451120912048E-5 20-21 1.0219107892319216E-4 0.0 0.0 3.4307005067071654E-4 2.9197451120912048E-5 22-23 1.0949044170342017E-4 0.0 0.0 0.001204394858737622 4.3796176681368075E-5 24-25 1.3138853004410423E-4 0.0 0.0 0.0033212100650037452 5.8394902241824096E-5 26-27 2.0438215784638432E-4 7.299362780228012E-6 0.0 0.0056205093407755684 5.8394902241824096E-5 28-29 2.1898088340684035E-4 1.4598725560456024E-5 0.0 0.01487610134610469 5.8394902241824096E-5 30-31 2.3357960896729638E-4 1.4598725560456024E-5 0.0 0.04430713207598403 5.8394902241824096E-5 32-33 2.3357960896729638E-4 1.4598725560456024E-5 0.0 0.09635888806178998 7.299362780228012E-5 34-35 2.481783345277524E-4 1.4598725560456024E-5 0.0 0.1611918282757752 7.299362780228012E-5 36-37 2.481783345277524E-4 1.4598725560456024E-5 0.0 0.2608354295886678 7.299362780228012E-5 38-39 2.481783345277524E-4 1.4598725560456024E-5 0.0 0.4134651053232355 8.029299058250812E-5 40-41 2.481783345277524E-4 1.4598725560456024E-5 0.0 0.5974017480222011 8.759235336273614E-5 42-43 2.554776973079804E-4 1.4598725560456024E-5 0.0 0.76383451877418 8.759235336273614E-5 44-45 2.627770600882084E-4 1.4598725560456024E-5 0.0 0.9540632121897022 8.759235336273614E-5 46-47 2.627770600882084E-4 1.4598725560456024E-5 0.0 1.164058580014082 8.759235336273614E-5 48-49 2.627770600882084E-4 1.4598725560456024E-5 0.0 1.3831708519509665 8.759235336273614E-5 50-51 2.627770600882084E-4 1.4598725560456024E-5 0.0 1.6260206516491524 8.759235336273614E-5 52-53 2.627770600882084E-4 1.4598725560456024E-5 0.0 1.8664397635415222 1.0219107892319216E-4 54-55 2.627770600882084E-4 1.4598725560456024E-5 0.0 2.105858862733001 1.240891672638762E-4 56-57 2.627770600882084E-4 1.4598725560456024E-5 0.0 2.379241896940881 1.313885300441042E-4 58-59 2.627770600882084E-4 1.4598725560456024E-5 0.0 2.6727054781571677 1.4598725560456023E-4 60-61 2.627770600882084E-4 2.1898088340684038E-5 0.0 2.9666946134936314 1.4598725560456023E-4 62-63 2.627770600882084E-4 2.9197451120912048E-5 0.0 3.2671582836161566 1.4598725560456023E-4 64-65 2.627770600882084E-4 2.9197451120912048E-5 0.0 3.5806002207619283 1.6058598116501627E-4 66-67 2.627770600882084E-4 2.9197451120912048E-5 0.0 3.9225169721133684 1.6058598116501627E-4 68-69 2.627770600882084E-4 2.9197451120912048E-5 0.0 4.294266219147601 1.6058598116501627E-4 70-71 2.627770600882084E-4 2.9197451120912048E-5 0.0 4.717512470596342 1.6058598116501627E-4 72-73 2.627770600882084E-4 2.9197451120912048E-5 0.0 5.12497020035145 1.897834322859283E-4 74-75 2.700764228684364E-4 2.9197451120912048E-5 0.0 5.5247854972756585 1.897834322859283E-4 76-77 2.7737578564866446E-4 2.9197451120912048E-5 0.0 5.959250869317611 1.897834322859283E-4 78-79 2.7737578564866446E-4 2.9197451120912048E-5 0.0 6.410176304428976 1.897834322859283E-4 80-81 2.7737578564866446E-4 2.9197451120912048E-5 0.0 6.900802973702001 1.897834322859283E-4 82-83 2.7737578564866446E-4 4.379617668136807E-5 0.0 7.422860699106689 1.897834322859283E-4 84-85 2.7737578564866446E-4 4.379617668136807E-5 0.0 7.933122654258528 1.897834322859283E-4 86-87 2.7737578564866446E-4 4.379617668136807E-5 0.0 8.45497599750537 1.897834322859283E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1725 0.0 23.182472 1 GTACATA 1895 0.0 20.857399 1 CTAGTAC 2725 0.0 19.623453 3 GTATAAG 2650 0.0 19.477245 1 GTACAAG 3320 0.0 17.927607 1 CGTTAAC 675 0.0 17.911007 1 TAGTACT 3015 0.0 17.735956 4 TAGTACC 1430 0.0 17.233915 4 TATTCGC 380 2.8194336E-10 17.13123 9 CATGGGG 15850 0.0 16.634047 4 GTGATCG 1820 0.0 16.351315 8 GTACATG 56125 0.0 16.313225 1 CCTAGTA 3280 0.0 16.161255 2 ATTGCGT 1215 0.0 16.073746 6 TACATGG 55095 0.0 15.934334 2 ATAGTAC 1695 0.0 15.9111805 3 GTACAAA 3495 0.0 15.699479 1 TGATCGC 1905 0.0 15.62173 9 CATGGGT 5005 0.0 15.608073 4 TCTAACG 1020 0.0 15.499682 2 >>END_MODULE