FastQCFastQC Report
Fri 27 May 2016
ERR765239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6849913
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT227940.3327633504250346No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA221670.3236099494986287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165270.2412731373376567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106340.15524284760988935No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG90940.1327608102467871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85630.1250088869741849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG8250.023.6725982
GTATCAA472250.023.566071
CTAACGC10250.021.3218353
AGTACTT269350.021.07310112-13
TCTAACG10800.020.6665552
CAACGCA549750.019.7925265
ATCAACG551400.019.7501663
ATCACGC9750.019.553743
AACGCAG566900.019.3985356
GTACTAG20250.019.2997741
TCAACGC569750.019.1140694
TATCAAC579700.019.0346572
TAACGCA4200.018.8213274
GAGTACT255950.018.76950612-13
TACTTTT311100.018.32728414-15
ACGCAGA603250.018.0523537
CGCAGAG606900.017.9437838
GTATAAG27100.017.511721
CATGGGG125600.016.9190964
GCAGAGT647650.016.7937169
Produced by FastQC (version 0.11.5)