##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765239_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6849913 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39603758471093 33.0 33.0 33.0 33.0 33.0 2 32.355364659375965 33.0 33.0 33.0 33.0 33.0 3 32.387746968465144 33.0 33.0 33.0 33.0 33.0 4 36.27366157789157 37.0 37.0 37.0 33.0 37.0 5 36.19117892446225 37.0 37.0 37.0 33.0 37.0 6 36.19411224638912 37.0 37.0 37.0 33.0 37.0 7 36.097002691859004 37.0 37.0 37.0 33.0 37.0 8 36.10727771287022 37.0 37.0 37.0 33.0 37.0 9 36.13899432007385 37.0 37.0 37.0 33.0 37.0 10-11 36.09049640484485 37.0 37.0 37.0 33.0 37.0 12-13 36.064862867601384 37.0 37.0 37.0 33.0 37.0 14-15 37.581110373226636 40.0 37.0 40.0 33.0 40.0 16-17 37.57513161116061 40.0 37.0 40.0 33.0 40.0 18-19 37.61431853222078 40.0 37.0 40.0 33.0 40.0 20-21 37.5915787543579 40.0 37.0 40.0 33.0 40.0 22-23 37.56358599007024 40.0 37.0 40.0 33.0 40.0 24-25 37.543308360266764 40.0 37.0 40.0 33.0 40.0 26-27 37.42084446911953 40.0 37.0 40.0 33.0 40.0 28-29 37.32213693808958 38.5 37.0 40.0 33.0 40.0 30-31 37.18167865781653 37.0 37.0 40.0 33.0 40.0 32-33 37.066911360772025 37.0 37.0 40.0 33.0 40.0 34-35 36.97115299128617 37.0 37.0 40.0 33.0 40.0 36-37 36.83444848131648 37.0 37.0 40.0 33.0 40.0 38-39 36.73072650703739 37.0 37.0 40.0 33.0 40.0 40-41 36.57478832212905 37.0 37.0 40.0 33.0 40.0 42-43 36.426673229280425 37.0 37.0 40.0 33.0 40.0 44-45 36.16569597307294 37.0 37.0 40.0 33.0 40.0 46-47 35.95427642657651 37.0 37.0 40.0 33.0 40.0 48-49 35.708426953743796 37.0 37.0 40.0 27.0 40.0 50-51 35.52155837599689 37.0 37.0 40.0 27.0 40.0 52-53 35.3771539142176 37.0 35.0 40.0 27.0 40.0 54-55 35.181108577583394 37.0 33.0 40.0 27.0 40.0 56-57 34.97875206590216 37.0 33.0 40.0 27.0 40.0 58-59 34.773106753326644 37.0 33.0 40.0 27.0 40.0 60-61 34.60559637764743 37.0 33.0 37.0 27.0 40.0 62-63 34.385514385365184 37.0 33.0 37.0 27.0 40.0 64-65 34.16256323255493 37.0 33.0 37.0 27.0 40.0 66-67 33.926514103171826 37.0 33.0 37.0 27.0 40.0 68-69 33.71402527302172 37.0 33.0 37.0 27.0 40.0 70-71 33.51636152167188 37.0 33.0 37.0 27.0 38.5 72-73 33.29772640907994 37.0 33.0 37.0 27.0 37.0 74-75 33.06372913057436 37.0 33.0 37.0 27.0 37.0 76-77 32.87695449562644 37.0 33.0 37.0 27.0 37.0 78-79 32.70665328158182 35.0 33.0 37.0 27.0 37.0 80-81 32.538454357011545 33.0 33.0 37.0 22.0 37.0 82-83 32.44150020591502 33.0 33.0 37.0 22.0 37.0 84-85 32.312037393759596 33.0 33.0 37.0 22.0 37.0 86-87 32.19836142444437 33.0 33.0 37.0 22.0 37.0 88-89 32.08462136672393 33.0 33.0 37.0 22.0 37.0 90-91 31.979605215423902 33.0 33.0 37.0 22.0 37.0 92-93 31.9033438818858 33.0 33.0 37.0 22.0 37.0 94-95 31.80328407090718 33.0 33.0 37.0 22.0 37.0 96-97 31.742703958429836 33.0 33.0 37.0 22.0 37.0 98-99 30.942369706009405 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.038512081655170505 1101 2 0.03738621478539983 1101 3 0.09523760101442491 1101 4 0.10676031866307767 1101 5 0.06001980215441449 1101 6 0.07775408903748371 1101 7 0.053730560326208376 1101 8 0.09700544622102569 1101 9 0.07502088631881065 1101 10-11 0.06690844320478817 1101 12-13 0.03301811224297779 1101 14-15 0.10771855421319287 1101 16-17 0.1600736729325547 1101 18-19 0.07144444897398472 1101 20-21 0.0903385435809696 1101 22-23 0.13031667989947948 1101 24-25 0.1584220580541782 1101 26-27 0.1742117375139003 1101 28-29 0.11593995856897976 1101 30-31 0.09598525766674726 1101 32-33 0.1431969802443689 1101 34-35 0.23965500865801204 1101 36-37 0.19249035067137754 1101 38-39 0.1734375009809881 1101 40-41 0.10561874554079509 1101 42-43 0.16984210412297784 1101 44-45 0.2189611545977499 1101 46-47 0.14834935439883168 1101 48-49 0.15500187157064005 1101 50-51 0.17363159389191196 1101 52-53 0.2406107626381626 1101 54-55 0.10907638437889489 1101 56-57 0.15997968631651105 1101 58-59 0.258625756520793 1101 60-61 0.11944982401966797 1101 62-63 0.21811607793350163 1101 64-65 0.12090063504400916 1101 66-67 0.17502162582573533 1101 68-69 0.22193608678232124 1101 70-71 0.05184994607908777 1101 72-73 0.0646658431498679 1101 74-75 0.16123281669137413 1101 76-77 0.23930553934113874 1101 78-79 0.18624042569972943 1101 80-81 0.16522182217556747 1101 82-83 0.160476536220024 1101 84-85 0.1207086547859717 1101 86-87 0.1393384915972291 1101 88-89 0.10642304857657336 1101 90-91 0.1728046932263787 1101 92-93 0.1263819844616627 1101 94-95 0.13468252128235392 1101 96-97 0.1041670572499882 1101 98-99 0.2522080119742185 1102 1 -0.013415831535326106 1102 2 0.07649770914188991 1102 3 0.07020827438096688 1102 4 0.05101042949585377 1102 5 0.04645658497489791 1102 6 0.09842638266066928 1102 7 0.08789384034564307 1102 8 0.14768204639108262 1102 9 0.06513840361725443 1102 10-11 0.05859992243892265 1102 12-13 0.08235866246915435 1102 14-15 0.09947358737291978 1102 16-17 0.05972100782760492 1102 18-19 0.14165788669077983 1102 20-21 0.15661895453527563 1102 22-23 0.19917396485364236 1102 24-25 0.14526125997875994 1102 26-27 0.13289792972579306 1102 28-29 0.10997128276971324 1102 30-31 0.13766573189625575 1102 32-33 0.12543450281803814 1102 34-35 0.14056965636594043 1102 36-37 0.13894434517018794 1102 38-39 0.12140089643985874 1102 40-41 0.15917153789105498 1102 42-43 0.16032138147686226 1102 44-45 0.1634049674484075 1102 46-47 0.18494912277922992 1102 48-49 0.21675834170963526 1102 50-51 0.20653027201989715 1102 52-53 0.17745051724708105 1102 54-55 0.10647736482372494 1102 56-57 0.13869154102700776 1102 58-59 0.24640491795032204 1102 60-61 0.16193305553881032 1102 62-63 0.27596627734086354 1102 64-65 0.13417385522195957 1102 66-67 0.1943953117208963 1102 68-69 0.23433579366680846 1102 70-71 0.1422737325895156 1102 72-73 0.10154619189806624 1102 74-75 0.28895243884085886 1102 76-77 0.2606170329187165 1102 78-79 0.21208068534185998 1102 80-81 0.23440203132659576 1102 82-83 0.2277959841756214 1102 84-85 0.10948947781943019 1102 86-87 0.16250051359639173 1102 88-89 0.22701050372275944 1102 90-91 0.10576236062281552 1102 92-93 0.15903121486352845 1102 94-95 0.229496750046021 1102 96-97 0.25317762151988177 1102 98-99 0.16899412145718884 1103 1 0.003205026866623939 1103 2 0.09317541769542714 1103 3 0.12526872597702976 1103 4 0.0825772684742958 1103 5 0.08386008625990371 1103 6 0.05665470307832976 1103 7 0.061118148157888186 1103 8 0.1216589526086409 1103 9 0.06281119344070163 1103 10-11 0.053076328616334933 1103 12-13 0.06765163763588333 1103 14-15 0.08204552412997401 1103 16-17 0.10781194495986313 1103 18-19 0.1483051334354144 1103 20-21 0.09729642435743813 1103 22-23 0.15499442753141324 1103 24-25 0.12794173649241003 1103 26-27 0.12709155758386004 1103 28-29 0.15447125076871515 1103 30-31 0.20303213154389965 1103 32-33 0.14333439146803073 1103 34-35 0.15032273879686642 1103 36-37 0.14274163800354955 1103 38-39 0.043485576072846754 1103 40-41 0.07100188896134796 1103 42-43 0.09631486998894445 1103 44-45 0.10353088983300296 1103 46-47 0.10811755926095401 1103 48-49 0.08820002565432361 1103 50-51 0.10912905035024778 1103 52-53 0.011943388933630672 1103 54-55 -0.012239031674305068 1103 56-57 0.024404029364596624 1103 58-59 0.0847343755449046 1103 60-61 0.0872767044681737 1103 62-63 0.09163678463212221 1103 64-65 0.13325447860924555 1103 66-67 0.10874170970706842 1103 68-69 0.10006987103876241 1103 70-71 -0.02418801269947579 1103 72-73 0.07468104556208033 1103 74-75 0.10567139250189683 1103 76-77 0.15350350835459636 1103 78-79 0.03404324512776924 1103 80-81 0.09095521589146927 1103 82-83 -0.023991864745333658 1103 84-85 -0.054328917722884285 1103 86-87 -0.05881910378946742 1103 88-89 0.043830508738579965 1103 90-91 -0.026197190340077725 1103 92-93 0.0668006785520383 1103 94-95 0.025042752420208814 1103 96-97 0.018484405636101542 1103 98-99 0.11346335896231352 1104 1 0.017655146229508034 1104 2 0.04057189110130821 1104 3 -0.02552917585692427 1104 4 -0.03627959866221886 1104 5 -0.04918376845747474 1104 6 0.04997507670863399 1104 7 0.030315434675188158 1104 8 0.0683539562852502 1104 9 0.05970098250841005 1104 10-11 0.03437426946290856 1104 12-13 -0.02138927422372916 1104 14-15 0.03708888982946945 1104 16-17 0.07467391587693584 1104 18-19 0.044124145550860305 1104 20-21 0.08025231699765811 1104 22-23 0.09295005962012226 1104 24-25 0.024327188709698078 1104 26-27 0.04405435267948121 1104 28-29 0.0016112478148571086 1104 30-31 -0.0500223931644328 1104 32-33 0.01454995091980038 1104 34-35 0.04900736876520284 1104 36-37 0.12840934177179975 1104 38-39 0.10110766408720195 1104 40-41 0.05285053585201638 1104 42-43 0.10551023476358523 1104 44-45 0.0795133274833617 1104 46-47 0.04746004616985289 1104 48-49 0.07873523266992066 1104 50-51 -0.05747594217565677 1104 52-53 0.09156839276897699 1104 54-55 0.009089268892076063 1104 56-57 0.15422702990942838 1104 58-59 0.13556891445483643 1104 60-61 0.08328464307158612 1104 62-63 0.04487588021492428 1104 64-65 0.05887528254500296 1104 66-67 0.1481286838929492 1104 68-69 0.10937220496887079 1104 70-71 -0.029490516366692532 1104 72-73 0.14878378778116996 1104 74-75 0.10194399141877852 1104 76-77 0.09181423653071619 1104 78-79 0.15183211751973147 1104 80-81 0.08744601617949144 1104 82-83 0.06496464132680302 1104 84-85 0.13691932537744833 1104 86-87 0.16444244970669786 1104 88-89 0.1003477209283119 1104 90-91 0.030075400723799106 1104 92-93 0.14373846799488987 1104 94-95 0.06637411678065419 1104 96-97 0.03670029362397287 1104 98-99 0.08325764218735543 1105 1 -0.003735044842891 1105 2 0.08701261002979521 1105 3 -6.728696097937359E-4 1105 4 0.04947541523426935 1105 5 0.04455594580466027 1105 6 0.03160717769647903 1105 7 0.04700362614695308 1105 8 0.007746959021318389 1105 9 0.025737231086537804 1105 10-11 -0.030032704237974883 1105 12-13 -0.020331120628675592 1105 14-15 -0.009649811019755816 1105 16-17 -0.008004811960049096 1105 18-19 0.006314851068346172 1105 20-21 -0.0200632000237988 1105 22-23 -0.0017756155862542755 1105 24-25 0.02458877332733067 1105 26-27 0.004543204297526415 1105 28-29 -0.0043740506984590866 1105 30-31 -0.012413017868702525 1105 32-33 -0.015254792667924733 1105 34-35 -0.004501062474076889 1105 36-37 0.04261983443113593 1105 38-39 -0.019774904997959197 1105 40-41 -0.06656077024783968 1105 42-43 -0.07476892527176915 1105 44-45 0.02001573401705059 1105 46-47 -0.07010212294366625 1105 48-49 -0.08902833672063792 1105 50-51 -0.019370303651044196 1105 52-53 -0.1777576792463833 1105 54-55 -0.12875575934270955 1105 56-57 -0.15059756980702588 1105 58-59 -0.08007050552669881 1105 60-61 -0.0966522703228847 1105 62-63 -0.14979706929820935 1105 64-65 -0.06667629058564017 1105 66-67 -0.012496072455100204 1105 68-69 -0.08542187756878405 1105 70-71 -0.10839414392350477 1105 72-73 -0.07957363384731764 1105 74-75 -0.006124697123674139 1105 76-77 -0.04537713813718369 1105 78-79 -0.09580782535105925 1105 80-81 -0.1005432545675049 1105 82-83 -0.14074397048789677 1105 84-85 -0.22688686603899555 1105 86-87 -0.12318501224888223 1105 88-89 -0.061369164497342865 1105 90-91 0.0073581779791318525 1105 92-93 -0.007782020942958212 1105 94-95 -0.13215981664901477 1105 96-97 -0.20435141540859547 1105 98-99 -0.004339369224489076 1106 1 -0.02594049426145517 1106 2 0.016026946420353738 1106 3 0.04006015636736038 1106 4 0.03126102177261458 1106 5 0.051304548706355035 1106 6 0.062030484051739165 1106 7 -0.012561881998202296 1106 8 0.011939942108014634 1106 9 -0.004056768388501553 1106 10-11 -0.027114318086645994 1106 12-13 -0.01378270222782163 1106 14-15 0.008345773594136574 1106 16-17 0.040277908893664005 1106 18-19 -0.04692856261107181 1106 20-21 -0.026242262772598224 1106 22-23 -0.036576487554214054 1106 24-25 0.022680050053544676 1106 26-27 -0.0065963334483569724 1106 28-29 -0.003297133234809735 1106 30-31 0.017032591958852095 1106 32-33 -0.020773371815408836 1106 34-35 0.02695943485044694 1106 36-37 0.011275276389064004 1106 38-39 -0.011472231042752412 1106 40-41 0.012066893115452615 1106 42-43 -0.024852816518659893 1106 44-45 0.02393324558646981 1106 46-47 0.02543838065432169 1106 48-49 -0.010149108531003037 1106 50-51 0.009102374649842204 1106 52-53 0.027709928004256312 1106 54-55 -0.004422260459627125 1106 56-57 0.1058058019621626 1106 58-59 0.056770277000829594 1106 60-61 -0.028518065021593486 1106 62-63 0.03534639263025241 1106 64-65 0.08936997839131777 1106 66-67 0.002724457238748812 1106 68-69 0.06802362661188965 1106 70-71 -0.02847633610716116 1106 72-73 0.0576545024219115 1106 74-75 0.06833108530038601 1106 76-77 0.14576840078715492 1106 78-79 0.0382296389931156 1106 80-81 0.040848103953351256 1106 82-83 -0.02003916965404784 1106 84-85 0.06004129299331851 1106 86-87 0.16326024796374128 1106 88-89 0.09479331604783425 1106 90-91 -0.05788614949134541 1106 92-93 0.036502101637918116 1106 94-95 0.029017950019504468 1106 96-97 0.048951764998154346 1106 98-99 0.016561695195058235 1107 1 -0.008554744087739152 1107 2 0.06227803769549922 1107 3 0.05823821683736696 1107 4 -0.03855386232950764 1107 5 0.004662290969434935 1107 6 -9.512359760535105E-4 1107 7 0.025094237160132593 1107 8 0.00286418534211208 1107 9 0.04307562778446794 1107 10-11 -0.0800557722887234 1107 12-13 -0.09005664346821618 1107 14-15 0.0036355182802978447 1107 16-17 -0.022999969845621138 1107 18-19 -0.04213572672104249 1107 20-21 -0.019705880995559255 1107 22-23 0.1082512245985825 1107 24-25 -0.007675794727042273 1107 26-27 0.03385509812646603 1107 28-29 -0.04284570165999213 1107 30-31 -0.04226213250530719 1107 32-33 0.010588895898877126 1107 34-35 -0.04044969979063495 1107 36-37 0.011369571469863615 1107 38-39 -0.07791734658428595 1107 40-41 -0.10179270208828228 1107 42-43 -0.028583151295151765 1107 44-45 -0.12322917178840953 1107 46-47 -0.02572563131852945 1107 48-49 -0.029725292898596933 1107 50-51 -0.02446716407483507 1107 52-53 -0.0990476040213295 1107 54-55 -0.13516548564536635 1107 56-57 -0.05673517653303861 1107 58-59 -0.04402252325572675 1107 60-61 -0.09123885893539097 1107 62-63 -0.13454692965670034 1107 64-65 -0.10706030471664008 1107 66-67 -0.10467331923246093 1107 68-69 -0.20201721825778662 1107 70-71 -0.0464953821990548 1107 72-73 -0.09589681868914113 1107 74-75 -0.03561830625803708 1107 76-77 -0.04774915760719978 1107 78-79 0.002142328073247768 1107 80-81 -0.015690059497387665 1107 82-83 -0.027757201973606982 1107 84-85 -0.011874407115357144 1107 86-87 -0.03175863777752852 1107 88-89 -0.06538565307575794 1107 90-91 -0.07178654502290627 1107 92-93 0.04882322159800978 1107 94-95 -0.027387033321847554 1107 96-97 -0.004917852120165378 1107 98-99 0.00989632024182896 1108 1 -0.06528104727478734 1108 2 -0.008258779803249183 1108 3 0.016166333277787714 1108 4 -0.04619340006358641 1108 5 -0.008355155910123813 1108 6 -0.04466693106243724 1108 7 -0.09585169918415914 1108 8 -0.06910408627642539 1108 9 -0.05427980753489692 1108 10-11 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-0.10878437221251858 2215 62-63 -0.17409498571382187 2215 64-65 -0.03011027407075062 2215 66-67 -0.11135056010910205 2215 68-69 -0.08435885358834838 2215 70-71 -0.17813841564448296 2215 72-73 -0.13681611234296298 2215 74-75 -0.24260693415076418 2215 76-77 -0.12094709687865901 2215 78-79 -0.10694504582308895 2215 80-81 -0.11625133469742366 2215 82-83 -0.12845808159326566 2215 84-85 -0.09711530860126771 2215 86-87 -0.09530121898911403 2215 88-89 -0.0363538144884501 2215 90-91 -0.11961006615200276 2215 92-93 -0.02870336577588617 2215 94-95 -0.01487369822154605 2215 96-97 -0.02997144100150706 2215 98-99 -0.06388061914558563 2216 1 -0.03608933115044266 2216 2 -0.0532827933857547 2216 3 -0.05594607394189666 2216 4 -0.12895502266967895 2216 5 -0.07097682588276655 2216 6 -0.11326094514720353 2216 7 -0.06865901699899268 2216 8 -0.07994567259060403 2216 9 -0.0033587435739761418 2216 10-11 -0.03165235573155911 2216 12-13 0.026838268886429262 2216 14-15 -0.14681953713338203 2216 16-17 -0.1083372866829535 2216 18-19 0.009216639827442918 2216 20-21 -0.018176922078538382 2216 22-23 -0.05836060690415934 2216 24-25 -0.1257621322174387 2216 26-27 -0.13241526361206013 2216 28-29 -0.034002725684977975 2216 30-31 -0.05569399971082589 2216 32-33 -0.01767793895099601 2216 34-35 -0.10837571570812798 2216 36-37 -0.08885406674202301 2216 38-39 -0.1001853095426597 2216 40-41 -0.04251565227795595 2216 42-43 -0.0812333694787668 2216 44-45 -0.1250294346005063 2216 46-47 -0.10041496759403401 2216 48-49 -0.032602961309081024 2216 50-51 -0.043087648541643375 2216 52-53 -0.05692316497638217 2216 54-55 0.014395047912763914 2216 56-57 -0.10187253304011534 2216 58-59 -0.13630003174753824 2216 60-61 -0.1013900998547328 2216 62-63 -0.008465836011303907 2216 64-65 -0.04901986636591005 2216 66-67 -0.04494685455094327 2216 68-69 0.04990053519473037 2216 70-71 -0.054782144899576224 2216 72-73 -0.055103299734440725 2216 74-75 -0.008768053363390038 2216 76-77 -0.003162156888180334 2216 78-79 0.11645371701034435 2216 80-81 -0.03668958222071694 2216 82-83 -0.011600208940606649 2216 84-85 -0.05992408107960756 2216 86-87 -0.11953787562246987 2216 88-89 -0.0972546251333739 2216 90-91 -0.08728734461628918 2216 92-93 0.10066718311835743 2216 94-95 0.004699471525299259 2216 96-97 -0.0033762174785572086 2216 98-99 -0.04002189189215599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 21.0 10 61.0 11 126.0 12 242.0 13 517.0 14 1203.0 15 3166.0 16 6986.0 17 13126.0 18 21105.0 19 28864.0 20 36762.0 21 46333.0 22 55316.0 23 54041.0 24 47357.0 25 47797.0 26 56968.0 27 73754.0 28 97338.0 29 127746.0 30 167604.0 31 219518.0 32 289229.0 33 381411.0 34 509586.0 35 730421.0 36 1230599.0 37 1900827.0 38 701068.0 39 817.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95269613854107 18.542513646004764 12.898006950775057 25.606783264679105 2 15.952611368932715 20.928426390232985 37.14280750719024 25.976154733644062 3 18.195982927082433 25.76959736568917 29.33400759980455 26.700412107423848 4 13.33662193957792 17.198218429927504 36.107947648386194 33.35721198210839 5 14.315303536976085 36.83091965174075 33.471737697450685 15.38203911383248 6 32.12507954480589 36.30253406138151 17.238978071692298 14.333408322120295 7 27.390012106723105 31.444632946433043 22.51165817726444 18.65369676957941 8 27.141089237191775 32.47988697082722 20.34948473068198 20.029539061299026 9 27.218155909425416 14.155946214207393 19.68614783866598 38.93975003770121 10-11 25.56352175567777 24.825702165852327 27.317916300542795 22.292859777927106 12-13 26.851392127170083 22.623849383196546 27.159724510369692 23.36503397926368 14-15 24.307431009531193 23.329887502202613 25.233381942861882 27.12929954540431 16-17 23.639146152961764 25.843132889389324 26.16867804083093 24.349042916817982 18-19 24.075153983152788 24.679254229632402 28.330921968949273 22.914669818265537 20-21 24.867764454302822 24.26102549264237 27.629228931584095 23.241981121470715 22-23 24.160759414024675 23.96884456780692 27.486093618999245 24.384302399169158 24-25 23.908573729330577 24.538151068488023 27.405698437337815 24.147576764843585 26-27 24.221088647403256 24.316980376247113 27.28315673498335 24.178774241366277 28-29 23.830996101702315 24.199073769258092 28.00592503875597 23.964005090283628 30-31 24.332460375658425 24.129917065857818 27.615329173754976 23.922293384728782 32-33 23.8555146612811 24.274330199522243 27.292835690029932 24.577319449166726 34-35 24.187737858860398 24.33998796773039 27.636000632416792 23.83627354099242 36-37 24.42476276705996 24.2576584549322 27.10008871645523 24.217490061552606 38-39 24.476121083581646 23.92087315561526 27.207615629570768 24.395390131232322 40-41 24.28541063222263 24.19547518340744 27.297412390493136 24.2217017938768 42-43 24.885206571236747 24.13313132590151 26.847355579552616 24.134306523309128 44-45 24.51117992301508 24.332068159113845 26.485307185653305 24.67144473221777 46-47 24.307410911642236 24.42366786264293 26.714762654649775 24.554158571065063 48-49 25.04472684543585 24.700839266133745 25.923460633733598 24.33097325469681 50-51 24.20147525961279 25.099282282855274 25.76189653795603 24.93734591957591 52-53 24.281790148283637 24.9914341977774 25.447615174090533 25.27916047984843 54-55 24.453427364697916 24.928177919923947 25.4971340511916 25.12126066418654 56-57 24.509698152370692 24.501960827823652 25.39633715055943 25.59200386924622 58-59 24.18905880674946 24.513938940228567 26.041491633978893 25.255510619043083 60-61 24.377784068206417 24.686028859052662 25.769495174610245 25.16669189813068 62-63 23.858587693011575 24.80218361897443 25.714377686256746 25.62485100175725 64-65 23.863960024017825 24.55826811231033 25.871189896864383 25.706581966807462 66-67 24.496624993631304 24.645313013464552 25.660939051342694 25.197122941561446 68-69 24.426346728783273 24.19461385859937 25.724545698609603 25.654493714007753 70-71 23.815346267901504 24.406528958834954 25.79529112267557 25.98283365058797 72-73 24.43875564550966 24.392141914795122 25.767407556855105 25.40169488284012 74-75 24.62581810375479 24.205944004669842 25.6763208803969 25.491917011178465 76-77 24.157760401547087 24.4588594019495 25.883083221646896 25.500296974856518 78-79 24.269862776319286 24.50373442808235 25.737640970809693 25.488761824788675 80-81 23.767934812004505 24.481498775568124 25.951656455707493 25.79890995671988 82-83 23.905955003074858 24.60748951172734 25.444376114293433 26.04217937090437 84-85 23.9193931522545 24.646149401388154 25.191734844773773 26.242722601583573 86-87 23.73474093346698 24.22597710727603 25.51595200020088 26.523329959056106 88-89 23.735185545828568 24.013437864330243 25.76486540383521 26.486511186005977 90-91 24.051863928357527 24.19160426564241 25.410156941450158 26.346374864549905 92-93 23.61483789628095 24.340981913080242 25.39402541266826 26.65015477797055 94-95 23.1230341270902 24.331980043654962 25.930715432298058 26.61427039695678 96-97 23.79124549729933 24.137380802706307 25.512220980008404 26.559152719985963 98-99 23.521350214912758 24.10661658300939 25.506321468027327 26.865711734050524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 111.0 2 348.5 3 584.5 4 406.5 5 395.5 6 822.0 7 2041.0 8 3689.5 9 3688.0 10 2286.0 11 1451.0 12 1393.0 13 1586.5 14 1877.0 15 2276.0 16 2674.5 17 3136.5 18 3882.0 19 4851.0 20 6090.0 21 7570.5 22 9418.5 23 11653.0 24 14601.5 25 18238.0 26 22470.0 27 27399.5 28 33000.5 29 39347.0 30 45623.5 31 52511.0 32 59764.5 33 67406.5 34 74647.5 35 82046.0 36 89558.5 37 98310.5 38 111081.0 39 119863.0 40 128280.5 41 138991.0 42 152055.0 43 171439.5 44 191770.0 45 214010.5 46 244904.5 47 275131.5 48 297785.5 49 325229.0 50 358069.5 51 349889.5 52 324107.5 53 317970.0 54 301637.5 55 286935.0 56 265708.0 57 246273.0 58 233595.0 59 205294.0 60 169312.0 61 131737.5 62 107618.0 63 85851.0 64 61929.0 65 47423.5 66 35228.5 67 26786.0 68 21395.5 69 18379.5 70 16538.5 71 14661.0 72 14889.5 73 12754.0 74 8369.0 75 6444.0 76 4894.5 77 3291.0 78 1854.5 79 1142.0 80 772.5 81 486.5 82 328.5 83 223.5 84 147.0 85 104.0 86 56.0 87 30.5 88 15.0 89 10.0 90 5.5 91 2.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05503719536291921 2 0.0 3 0.0 4 0.0 5 0.006102267284270618 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013138853004410421 16-17 0.004751885169928436 18-19 8.248279941657653E-4 20-21 2.1898088340684038E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.9197451120912047E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.9197451120912048E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.649681390114006E-5 76-77 9.489171614296415E-5 78-79 2.9197451120912048E-5 80-81 2.1898088340684034E-5 82-83 7.299362780228012E-6 84-85 3.4307005067071654E-4 86-87 7.445350035832571E-4 88-89 2.1898088340684038E-4 90-91 9.562165242098695E-4 92-93 0.0014744712816060583 94-95 8.978216219680455E-4 96-97 0.001970827950661563 98-99 5.912483851984689E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6849913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.803401528200155 #Duplication Level Percentage of deduplicated Percentage of total 1 70.91991234033094 27.519338348866185 2 15.350431466211617 11.912979116290552 3 5.557099605673247 6.469031019934253 4 2.5349709980108175 3.9346198999262407 5 1.446468387304863 2.8063946815219367 6 0.8427145164385983 1.9620113853005976 7 0.5862986157393714 1.5925266421373934 8 0.4018765352692281 1.2475341250251 9 0.30440269072125786 1.0630673850889347 >10 1.7403056919026585 12.350910075245565 >50 0.1509552313871028 4.09785862385033 >100 0.1347162329714535 10.907984763718275 >500 0.019055782641095392 5.0571456375569985 >1k 0.010376832113294229 7.2918874665562825 >5k 2.6413754467473214E-4 0.7306968777923957 >10k+ 1.5093573981413263E-4 1.0560139511891464 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 22794 0.3327633504250346 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 22167 0.3236099494986287 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16527 0.2412731373376567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10634 0.15524284760988935 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9094 0.1327608102467871 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8563 0.1250088869741849 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4598725560456024E-5 0.0 2 0.0 0.0 0.0 2.9197451120912048E-5 0.0 3 0.0 0.0 0.0 2.9197451120912048E-5 0.0 4 0.0 0.0 0.0 2.9197451120912048E-5 0.0 5 0.0 1.4598725560456024E-5 0.0 2.9197451120912048E-5 0.0 6 0.0 1.4598725560456024E-5 0.0 2.9197451120912048E-5 0.0 7 1.4598725560456024E-5 1.4598725560456024E-5 0.0 2.9197451120912048E-5 0.0 8 1.4598725560456024E-5 1.4598725560456024E-5 0.0 4.379617668136807E-5 4.379617668136807E-5 9 1.4598725560456024E-5 1.4598725560456024E-5 0.0 4.379617668136807E-5 4.379617668136807E-5 10-11 1.4598725560456024E-5 1.4598725560456024E-5 0.0 8.759235336273614E-5 4.379617668136807E-5 12-13 2.1898088340684038E-5 1.4598725560456024E-5 0.0 1.0219107892319216E-4 4.379617668136807E-5 14-15 2.9197451120912048E-5 1.4598725560456024E-5 0.0 1.1678980448364819E-4 7.299362780228012E-5 16-17 2.9197451120912048E-5 1.4598725560456024E-5 0.0 1.4598725560456023E-4 1.0219107892319216E-4 18-19 2.9197451120912048E-5 1.4598725560456024E-5 0.0 1.970827950661563E-4 1.0219107892319216E-4 20-21 2.9197451120912048E-5 1.4598725560456024E-5 0.0 3.4307005067071654E-4 1.0219107892319216E-4 22-23 2.9197451120912048E-5 1.4598725560456024E-5 0.0 0.001182496770396938 1.240891672638762E-4 24-25 2.9197451120912048E-5 1.4598725560456024E-5 0.0 0.0032774138883223773 1.313885300441042E-4 26-27 6.569426502205211E-5 1.4598725560456024E-5 0.0 0.0055548150757535174 1.313885300441042E-4 28-29 7.299362780228012E-5 1.4598725560456024E-5 0.0 0.014781209629961725 1.313885300441042E-4 30-31 8.759235336273614E-5 1.4598725560456024E-5 0.0 0.04414654609481901 1.313885300441042E-4 32-33 8.759235336273614E-5 1.4598725560456024E-5 0.0 0.09606691355058086 1.4598725560456023E-4 34-35 8.759235336273614E-5 1.4598725560456024E-5 0.0 0.16089985376456606 1.4598725560456023E-4 36-37 8.759235336273614E-5 1.4598725560456024E-5 0.0 0.26028797738015064 1.4598725560456023E-4 38-39 9.489171614296415E-5 1.4598725560456024E-5 0.0 0.41253078688736633 1.6058598116501627E-4 40-41 1.0219107892319216E-4 1.4598725560456024E-5 0.0 0.5962922448796064 1.6058598116501627E-4 42-43 1.0219107892319216E-4 1.4598725560456024E-5 0.0 0.7623162513158926 1.6058598116501627E-4 44-45 1.0219107892319216E-4 1.4598725560456024E-5 0.0 0.9522383714946452 1.6058598116501627E-4 46-47 1.0219107892319216E-4 1.4598725560456024E-5 0.0 1.161949064170596 1.7518470672547227E-4 48-49 1.0219107892319216E-4 1.4598725560456024E-5 0.0 1.3808642533124145 1.897834322859283E-4 50-51 1.0219107892319216E-4 1.4598725560456024E-5 0.0 1.6234731740388528 1.897834322859283E-4 52-53 1.0219107892319216E-4 1.4598725560456024E-5 0.0 1.863709801861717 2.0438215784638432E-4 54-55 1.0219107892319216E-4 1.4598725560456024E-5 0.0 2.1033332832110423 2.1898088340684035E-4 56-57 1.0219107892319216E-4 1.4598725560456024E-5 0.0 2.377256470264659 2.1898088340684035E-4 58-59 1.0219107892319216E-4 1.4598725560456024E-5 0.0 2.671464586484529 2.1898088340684035E-4 60-61 1.0219107892319216E-4 1.4598725560456024E-5 0.0 2.9657602950577617 2.1898088340684035E-4 62-63 1.0949044170342017E-4 1.4598725560456024E-5 0.0 3.2668882071932885 2.1898088340684035E-4 64-65 1.1678980448364819E-4 1.4598725560456024E-5 0.0 3.5813228576771703 2.1898088340684035E-4 66-67 1.1678980448364819E-4 1.4598725560456024E-5 0.0 3.9246264879568544 2.1898088340684035E-4 68-69 1.1678980448364819E-4 1.4598725560456024E-5 0.0 4.296908588474043 2.1898088340684035E-4 70-71 1.1678980448364819E-4 1.4598725560456024E-5 0.0 4.720570903601258 2.1898088340684035E-4 72-73 1.1678980448364819E-4 1.4598725560456024E-5 0.0 5.12818191997475 2.627770600882084E-4 74-75 1.1678980448364819E-4 1.4598725560456024E-5 0.0 5.529041025776532 2.627770600882084E-4 76-77 1.1678980448364819E-4 1.4598725560456024E-5 0.0 5.964148741743143 2.627770600882084E-4 78-79 1.1678980448364819E-4 1.4598725560456024E-5 0.0 6.4163004698015875 2.627770600882084E-4 80-81 1.1678980448364819E-4 1.4598725560456024E-5 0.0 6.908255623100614 2.7737578564866446E-4 82-83 1.1678980448364819E-4 1.4598725560456024E-5 0.0 7.431583437629062 3.0657323676957647E-4 84-85 1.1678980448364819E-4 1.4598725560456024E-5 0.0 7.943553443671474 3.0657323676957647E-4 86-87 1.1678980448364819E-4 1.4598725560456024E-5 0.0 8.468107551147 3.0657323676957647E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 825 0.0 23.672598 2 GTATCAA 47225 0.0 23.56607 1 CTAACGC 1025 0.0 21.321835 3 AGTACTT 26935 0.0 21.073101 12-13 TCTAACG 1080 0.0 20.666555 2 CAACGCA 54975 0.0 19.792526 5 ATCAACG 55140 0.0 19.750166 3 ATCACGC 975 0.0 19.55374 3 AACGCAG 56690 0.0 19.398535 6 GTACTAG 2025 0.0 19.299774 1 TCAACGC 56975 0.0 19.114069 4 TATCAAC 57970 0.0 19.034657 2 TAACGCA 420 0.0 18.821327 4 GAGTACT 25595 0.0 18.769506 12-13 TACTTTT 31110 0.0 18.327284 14-15 ACGCAGA 60325 0.0 18.052353 7 CGCAGAG 60690 0.0 17.943783 8 GTATAAG 2710 0.0 17.51172 1 CATGGGG 12560 0.0 16.919096 4 GCAGAGT 64765 0.0 16.793716 9 >>END_MODULE