FastQCFastQC Report
Fri 27 May 2016
ERR765238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6506491
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA173250.2662725576658755No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155570.2390996929066681No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG69660.1070623166926689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22300.025.020431
GTATAAG26450.019.5126251
TAGGCAT32650.019.082565
TAGTACT31350.018.9839884
CTAGTAC32700.017.9160063
TACTAGG32600.017.8283372
GTACAAG33200.017.7862361
GTGATCG17150.017.6220978
GTATATA19000.017.619651
CTAACGC10500.017.2700563
CATGGGG146750.017.1726064
CTAGGCA38400.017.072734
GTATAAT20450.016.59771
GTACATG455400.016.4074551
TACATGG446500.016.3283772
ATAAGGT23250.016.198713
CCTAGTA35150.016.0058382
TCTAACG10850.015.8558822
GTATCAA315600.015.734491
ACATGGG432650.015.5399633
Produced by FastQC (version 0.11.5)