##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6506491 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94500368939264 33.0 33.0 33.0 27.0 33.0 2 30.93655674003084 33.0 33.0 33.0 27.0 33.0 3 30.88212110029815 33.0 33.0 33.0 27.0 33.0 4 34.40680176150247 37.0 37.0 37.0 27.0 37.0 5 34.515592967084714 37.0 37.0 37.0 27.0 37.0 6 34.45353939627366 37.0 37.0 37.0 27.0 37.0 7 34.34671023136742 37.0 37.0 37.0 27.0 37.0 8 34.32806208446304 37.0 37.0 37.0 27.0 37.0 9 34.22201951866221 37.0 37.0 37.0 27.0 37.0 10-11 34.205482186942234 37.0 35.0 37.0 27.0 37.0 12-13 34.110836240302184 37.0 33.0 37.0 27.0 37.0 14-15 35.389059479218524 37.0 37.0 40.0 27.0 40.0 16-17 35.425708573177154 37.0 37.0 40.0 27.0 40.0 18-19 35.41208686832887 37.0 33.0 40.0 27.0 40.0 20-21 35.40538609828247 37.0 33.0 40.0 27.0 40.0 22-23 35.385467911966686 37.0 33.0 40.0 27.0 40.0 24-25 35.450974803469336 37.0 33.0 40.0 27.0 40.0 26-27 35.392012299717315 37.0 33.0 40.0 27.0 40.0 28-29 35.3939552056554 37.0 33.0 40.0 27.0 40.0 30-31 35.30196399257295 37.0 33.0 40.0 27.0 40.0 32-33 35.21329215701674 37.0 33.0 40.0 27.0 40.0 34-35 35.07290696321566 37.0 33.0 40.0 27.0 40.0 36-37 34.871985068449334 37.0 33.0 40.0 27.0 40.0 38-39 34.580825901396004 37.0 33.0 40.0 24.5 40.0 40-41 34.688625404999414 37.0 33.0 40.0 27.0 40.0 42-43 34.60759624504207 37.0 33.0 40.0 27.0 40.0 44-45 34.506183209966785 37.0 33.0 40.0 24.5 40.0 46-47 34.40036226900183 37.0 33.0 40.0 22.0 40.0 48-49 34.25541778202721 37.0 33.0 40.0 22.0 40.0 50-51 34.065012539016806 37.0 33.0 40.0 22.0 40.0 52-53 33.92285603714814 37.0 33.0 40.0 22.0 40.0 54-55 33.72203842286111 37.0 33.0 37.0 22.0 40.0 56-57 33.50847937851601 37.0 33.0 37.0 22.0 40.0 58-59 33.28531446520098 37.0 33.0 37.0 22.0 40.0 60-61 33.06498110886498 37.0 33.0 37.0 22.0 40.0 62-63 32.829786439418726 37.0 33.0 37.0 22.0 40.0 64-65 32.6113727045807 37.0 33.0 37.0 22.0 40.0 66-67 32.39784493669475 37.0 33.0 37.0 22.0 40.0 68-69 32.20756871868416 33.0 33.0 37.0 22.0 38.5 70-71 31.953487448149858 33.0 30.0 37.0 22.0 37.0 72-73 31.60861691808995 33.0 27.0 37.0 22.0 37.0 74-75 31.367314501779838 33.0 27.0 37.0 22.0 37.0 76-77 31.182302104160293 33.0 27.0 37.0 22.0 37.0 78-79 30.91848517119289 33.0 27.0 37.0 22.0 37.0 80-81 30.71077874387285 33.0 27.0 37.0 15.0 37.0 82-83 30.58775605775832 33.0 27.0 37.0 15.0 37.0 84-85 30.44510528025014 33.0 27.0 37.0 15.0 37.0 86-87 30.168245833276337 33.0 27.0 37.0 15.0 37.0 88-89 30.02372307899911 33.0 27.0 37.0 15.0 37.0 90-91 29.872132229184672 33.0 27.0 37.0 15.0 37.0 92-93 29.613459697400643 33.0 27.0 37.0 15.0 37.0 94-95 29.479702961242857 33.0 27.0 37.0 15.0 37.0 96-97 29.288254144976147 33.0 27.0 37.0 15.0 37.0 98-99 28.27428862961618 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3364582587704845 1101 2 0.36668512800182285 1101 3 0.37319106730874907 1101 4 0.3404643309841404 1101 5 0.36435960739929385 1101 6 0.5079449166718817 1101 7 0.4460163531887247 1101 8 0.535278102006572 1101 9 0.4389635077038747 1101 10-11 0.3955397821659048 1101 12-13 0.5610690373612144 1101 14-15 0.7026724532711413 1101 16-17 0.5834241400423181 1101 18-19 0.5263706422075174 1101 20-21 0.5491106725611274 1101 22-23 0.5238394926597678 1101 24-25 0.42212060203609525 1101 26-27 0.47188861542954896 1101 28-29 0.44573446331946087 1101 30-31 0.6615917701659697 1101 32-33 0.7061192842293167 1101 34-35 0.8374507669554418 1101 36-37 0.9653227336261381 1101 38-39 0.9761376401374235 1101 40-41 0.6570848859049718 1101 42-43 0.5698907171787368 1101 44-45 0.5317873567886906 1101 46-47 0.5436821542242996 1101 48-49 0.3942818302880795 1101 50-51 0.3547780511294292 1101 52-53 0.5741434564909937 1101 54-55 0.34888488647078475 1101 56-57 0.46019165399290074 1101 58-59 0.4019455779506771 1101 60-61 0.21975000432358627 1101 62-63 0.406741910137157 1101 64-65 0.4769041344729459 1101 66-67 0.4100609568873068 1101 68-69 0.5547154185002725 1101 70-71 0.5477512328493646 1101 72-73 0.3670721764146023 1101 74-75 0.3846370476715286 1101 76-77 0.3382597586245133 1101 78-79 0.3851973504710422 1101 80-81 0.44842221377738056 1101 82-83 0.4735139285964678 1101 84-85 0.4163369731712123 1101 86-87 0.413328325613012 1101 88-89 0.31534214850439923 1101 90-91 0.3398592594614698 1101 92-93 0.40691431816117074 1101 94-95 0.41934904697738773 1101 96-97 0.31667799217674286 1101 98-99 0.25986384029272 1102 1 0.28423455702719025 1102 2 0.3853554652878195 1102 3 0.295004974379669 1102 4 0.34233448246943254 1102 5 0.29873925694501935 1102 6 0.5319923896578516 1102 7 0.45376505689082336 1102 8 0.5602716539664669 1102 9 0.37617379346166757 1102 10-11 0.34430374344474757 1102 12-13 0.5046307525486711 1102 14-15 0.6046635052466414 1102 16-17 0.490120183137968 1102 18-19 0.505519262423249 1102 20-21 0.5153661492074946 1102 22-23 0.5017856879266489 1102 24-25 0.4063700470272451 1102 26-27 0.5484011336309464 1102 28-29 0.44634110150712303 1102 30-31 0.693884359046848 1102 32-33 0.7745147365090261 1102 34-35 0.8343939101709452 1102 36-37 0.9349190729149512 1102 38-39 0.9158677234407833 1102 40-41 0.6799438319340325 1102 42-43 0.5654893461447159 1102 44-45 0.4740736393837466 1102 46-47 0.4261409997313592 1102 48-49 0.4041823043336663 1102 50-51 0.470184877127906 1102 52-53 0.5169759013788351 1102 54-55 0.37850656920593906 1102 56-57 0.461170292480908 1102 58-59 0.38502517844565176 1102 60-61 0.3646300427199378 1102 62-63 0.37964099859220823 1102 64-65 0.42716995830256366 1102 66-67 0.3786193543544982 1102 68-69 0.4924136834320407 1102 70-71 0.5765599798968708 1102 72-73 0.38567153186604486 1102 74-75 0.3618173300988268 1102 76-77 0.42701960609774403 1102 78-79 0.37146594598425864 1102 80-81 0.281464081878557 1102 82-83 0.26671867111269165 1102 84-85 0.49903659994914307 1102 86-87 0.43268381558051416 1102 88-89 0.3409995219528952 1102 90-91 0.33071980909370424 1102 92-93 0.4293563034933534 1102 94-95 0.5261755720639769 1102 96-97 0.3115810567452115 1102 98-99 0.34252716478285095 1103 1 0.21214399717984378 1103 2 0.2541845500028863 1103 3 0.1415751449297744 1103 4 0.1787057617776 1103 5 0.23952356288453558 1103 6 0.35553825203447786 1103 7 0.2641120270711852 1103 8 0.2864384260036985 1103 9 0.22781446851540466 1103 10-11 0.2520656950992688 1103 12-13 0.32314113021096347 1103 14-15 0.5185431267540892 1103 16-17 0.3525037075884825 1103 18-19 0.31210395477543784 1103 20-21 0.32457276675268076 1103 22-23 0.272054646132311 1103 24-25 0.21938576165977963 1103 26-27 0.34022608435117263 1103 28-29 0.2844403263042494 1103 30-31 0.48325420573858935 1103 32-33 0.5398108587021966 1103 34-35 0.5787538569259283 1103 36-37 0.7712612814412836 1103 38-39 0.6803446875354098 1103 40-41 0.5736082230197681 1103 42-43 0.43921321921391154 1103 44-45 0.3370170344170518 1103 46-47 0.29973591885783435 1103 48-49 0.3072003452511396 1103 50-51 0.19615769699976227 1103 52-53 0.24428568158027986 1103 54-55 0.1432485623605686 1103 56-57 0.29535037651927354 1103 58-59 0.25826341126741426 1103 60-61 0.18396272154834747 1103 62-63 0.2130365242083343 1103 64-65 0.31649872105548127 1103 66-67 0.33580660388157924 1103 68-69 0.33606671901915064 1103 70-71 0.4446027012597966 1103 72-73 0.2597340626872935 1103 74-75 0.34137539944456563 1103 76-77 0.22201143080262042 1103 78-79 0.21450204304699838 1103 80-81 0.10164493253496332 1103 82-83 0.2577331439949475 1103 84-85 0.3918101025326237 1103 86-87 0.2681751811900632 1103 88-89 0.20805140941373068 1103 90-91 0.20462358388260427 1103 92-93 0.2880419933742786 1103 94-95 0.30762135638879684 1103 96-97 0.16754258875145212 1103 98-99 0.1683379807858465 1104 1 0.12884029802003027 1104 2 0.12172670651102635 1104 3 0.11059128715881528 1104 4 0.101924242094519 1104 5 0.10185459903742355 1104 6 0.17232603437343386 1104 7 0.11420326677457382 1104 8 0.147084288098128 1104 9 0.0857885174603652 1104 10-11 0.14573891736184663 1104 12-13 0.1878186326868274 1104 14-15 0.2977551498604427 1104 16-17 0.12498771965803712 1104 18-19 0.27891758436827985 1104 20-21 0.10718567046852456 1104 22-23 0.0871653315178591 1104 24-25 0.0020008503360884333 1104 26-27 0.1777410074499315 1104 28-29 0.014499955117990737 1104 30-31 0.30795567671300716 1104 32-33 0.36749774678447267 1104 34-35 0.434411247172072 1104 36-37 0.5361229709067743 1104 38-39 0.43968684067395003 1104 40-41 0.25378304015332986 1104 42-43 0.20366118486194296 1104 44-45 0.1216065455319395 1104 46-47 0.06278606899206807 1104 48-49 -0.0892617719405493 1104 50-51 -0.18279945927025665 1104 52-53 -0.06555784076735449 1104 54-55 -0.2191098686402384 1104 56-57 0.02412984631593673 1104 58-59 -0.021598865393251288 1104 60-61 -0.20361531532432053 1104 62-63 -0.11763861832383782 1104 64-65 0.032886011531886084 1104 66-67 -0.016215193366868164 1104 68-69 -0.02818609859350829 1104 70-71 0.06264783100725424 1104 72-73 -0.1500381916346889 1104 74-75 -0.05652813927287781 1104 76-77 -0.012546741662479377 1104 78-79 0.01206809649881535 1104 80-81 -0.10136804406950262 1104 82-83 0.04036307694057939 1104 84-85 0.08080794535532476 1104 86-87 0.03891813184744919 1104 88-89 -0.05621984367341781 1104 90-91 -0.22968884162616732 1104 92-93 -0.18776142306096943 1104 94-95 -0.11101296296334695 1104 96-97 -0.2778166396149473 1104 98-99 -0.2590738580527514 1105 1 0.07728058451725417 1105 2 0.07868264934690217 1105 3 0.10598790312784345 1105 4 -0.0762545093614051 1105 5 -0.1743399851598042 1105 6 0.13105687777138542 1105 7 0.061487270196295185 1105 8 0.03750502048323767 1105 9 8.258761570161255E-4 1105 10-11 0.06269676921261436 1105 12-13 0.13854278969967737 1105 14-15 0.16036799922225953 1105 16-17 0.04974836785317649 1105 18-19 0.16051153931553586 1105 20-21 0.06937690543201569 1105 22-23 0.06759521025916371 1105 24-25 -0.09755850552845402 1105 26-27 0.03175060327040313 1105 28-29 0.03663425794091779 1105 30-31 0.2239744261390939 1105 32-33 0.33486809084211444 1105 34-35 0.30837246517191375 1105 36-37 0.42887952801048357 1105 38-39 0.3377415182437531 1105 40-41 0.1686554744614739 1105 42-43 0.045177909282351436 1105 44-45 -0.037313205764498036 1105 46-47 -0.028630673793550443 1105 48-49 -0.1740546559062821 1105 50-51 -0.1253378087214756 1105 52-53 -0.11442499749506396 1105 54-55 -0.1913696469045334 1105 56-57 0.07134202003145873 1105 58-59 -0.1402737432404635 1105 60-61 -0.255343577927448 1105 62-63 -0.0973589597233584 1105 64-65 0.018127091116056704 1105 66-67 -0.07688713036453976 1105 68-69 0.030346352243320496 1105 70-71 0.008402508093759309 1105 72-73 -0.18124937701211863 1105 74-75 -0.12644883057791745 1105 76-77 -0.09413175565386922 1105 78-79 -0.17872676655957065 1105 80-81 -0.2984593336453152 1105 82-83 -0.1834006546760918 1105 84-85 -0.13104830582270566 1105 86-87 -0.19573906707190147 1105 88-89 -0.27816668848258885 1105 90-91 -0.2555967715290741 1105 92-93 -0.25726557456426136 1105 94-95 -0.2219557263469909 1105 96-97 -0.32362937250268686 1105 98-99 -0.253898977789067 1106 1 0.12246832295068799 1106 2 0.15984639912734622 1106 3 0.0879069088659854 1106 4 -0.013517607717140834 1106 5 0.0603630316350916 1106 6 0.1645159799725704 1106 7 0.13926168204347533 1106 8 0.1350893055079183 1106 9 0.19575107200371633 1106 10-11 0.15242138603115762 1106 12-13 0.16876103235471618 1106 14-15 0.25544779460411604 1106 16-17 0.03836231167220916 1106 18-19 0.270959335834398 1106 20-21 0.12963426156220947 1106 22-23 0.0399167634761497 1106 24-25 0.07302692731660443 1106 26-27 0.16838299210878915 1106 28-29 0.1661408405478184 1106 30-31 0.3729273223732008 1106 32-33 0.3029250690590075 1106 34-35 0.3920351781416045 1106 36-37 0.5093287283686934 1106 38-39 0.548754685980505 1106 40-41 0.3021416743062062 1106 42-43 0.21936986367902023 1106 44-45 0.16163036758270977 1106 46-47 0.1253669134289055 1106 48-49 -0.03515235135110828 1106 50-51 -0.09829940936023718 1106 52-53 0.0317889252753929 1106 54-55 -0.12146633160906362 1106 56-57 -0.039392686529474474 1106 58-59 -0.08525936965556014 1106 60-61 -0.14963123127752453 1106 62-63 0.08621069557731431 1106 64-65 0.05479013975774194 1106 66-67 0.024335828758196953 1106 68-69 0.03099784302819586 1106 70-71 0.0373448007454229 1106 72-73 -0.11237787162298574 1106 74-75 0.01785492157169344 1106 76-77 0.0758712070058074 1106 78-79 -0.01852774755313291 1106 80-81 0.037330207949775485 1106 82-83 -0.0024141959290204795 1106 84-85 -0.017701989528390527 1106 86-87 -0.0552231882492471 1106 88-89 -0.1418173802143663 1106 90-91 -0.20560490803055487 1106 92-93 -0.18428099108706064 1106 94-95 -0.09326468609783234 1106 96-97 -0.2032071682694685 1106 98-99 -0.2114982839548354 1107 1 0.10002088599325631 1107 2 0.20321322977334688 1107 3 0.09392840580600748 1107 4 0.04446987455823859 1107 5 0.0650448885217969 1107 6 0.10356840262245015 1107 7 0.07823427616523304 1107 8 0.06200092830592041 1107 9 -0.01970692418228026 1107 10-11 0.0989684994429254 1107 12-13 0.1304012895846256 1107 14-15 0.16949774473913948 1107 16-17 0.014618275008714932 1107 18-19 0.2983021549663931 1107 20-21 0.13560796493069915 1107 22-23 -0.02402080396823436 1107 24-25 0.08524793536285813 1107 26-27 0.11938669704989024 1107 28-29 0.10283580201470244 1107 30-31 0.23584614861357522 1107 32-33 0.3090628671412645 1107 34-35 0.3562700696262411 1107 36-37 0.48149783593929385 1107 38-39 0.4610190114810706 1107 40-41 0.28644383555093356 1107 42-43 0.10545765054436629 1107 44-45 0.1930334362583963 1107 46-47 0.10473993178677432 1107 48-49 -0.012023936123718215 1107 50-51 -0.009285689493914617 1107 52-53 0.045805882905952444 1107 54-55 0.03862458455430584 1107 56-57 -0.01015570752144157 1107 58-59 -0.0073145038818367425 1107 60-61 -0.036651784170004476 1107 62-63 0.032989568118630075 1107 64-65 0.15426456726811466 1107 66-67 0.11436831645447398 1107 68-69 0.1264860213994652 1107 70-71 0.18649770468292814 1107 72-73 -0.22651857974354428 1107 74-75 0.015961249837982194 1107 76-77 0.044694606785764535 1107 78-79 0.034267522566633346 1107 80-81 -0.037138724507901344 1107 82-83 0.04010843940328357 1107 84-85 0.029669062769691834 1107 86-87 -0.08676153151844446 1107 88-89 -0.19061686516208454 1107 90-91 -0.072191034171599 1107 92-93 -0.2136260714323761 1107 94-95 -0.1867250965864784 1107 96-97 -0.1889167424593694 1107 98-99 -0.09260209327851143 1108 1 -0.00787516781894837 1108 2 -0.01300418889313093 1108 3 -0.07753592220741012 1108 4 -0.10363789934044831 1108 5 -0.028326601967705756 1108 6 0.06017149543023237 1108 7 -0.0052664133318387485 1108 8 0.0810719356052303 1108 9 0.031456272561968035 1108 10-11 -0.006026846932400076 1108 12-13 -0.011415205154897023 1108 14-15 -0.09133830446629077 1108 16-17 -0.13271369485550366 1108 18-19 0.10254336057143831 1108 20-21 0.044563085221106746 1108 22-23 -0.06491784736086004 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-0.2701835587592072 2216 10-11 -0.44454189193942995 2216 12-13 -0.5398367813927436 2216 14-15 -0.4691519444964527 2216 16-17 -0.500520823347621 2216 18-19 -0.4540018128929759 2216 20-21 -0.16705088775358945 2216 22-23 -0.2006134819728942 2216 24-25 -0.3313758091710355 2216 26-27 -0.39315185762437466 2216 28-29 -0.4685291040865067 2216 30-31 -0.31727413431543994 2216 32-33 -0.18366941182590324 2216 34-35 -0.36385600036665267 2216 36-37 -0.20470787587113648 2216 38-39 -8.675825815278468 2216 40-41 -2.0709505491242552 2216 42-43 -0.7157641571686213 2216 44-45 -0.6826783413772333 2216 46-47 -0.37827974760651983 2216 48-49 -0.23877188328995658 2216 50-51 -0.2183376254374636 2216 52-53 -0.23812310050372076 2216 54-55 -0.2430432967995131 2216 56-57 -0.16674682389639628 2216 58-59 -0.2881080151021749 2216 60-61 -0.30251138929381227 2216 62-63 -0.3326270855696265 2216 64-65 -0.3985009555215413 2216 66-67 -0.2375943171156294 2216 68-69 -0.2750624975885074 2216 70-71 -0.09678904298453972 2216 72-73 -0.061351959673352496 2216 74-75 -0.13660434488931728 2216 76-77 -0.2578414328937342 2216 78-79 -0.19557001889257464 2216 80-81 -0.1543073139341793 2216 82-83 -0.28791860178242246 2216 84-85 -0.21646834948146676 2216 86-87 -0.07331821174801334 2216 88-89 -0.0263294016898179 2216 90-91 -0.04076660710613922 2216 92-93 -0.028999952264044282 2216 94-95 0.10436162422015727 2216 96-97 0.1895005842057884 2216 98-99 0.17203356538653836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 7.0 7 241.0 8 1690.0 9 3488.0 10 5791.0 11 9848.0 12 16197.0 13 24592.0 14 30980.0 15 35192.0 16 38574.0 17 42224.0 18 46677.0 19 51865.0 20 56752.0 21 63713.0 22 71107.0 23 80096.0 24 90067.0 25 100632.0 26 111748.0 27 124843.0 28 145323.0 29 175540.0 30 213744.0 31 267321.0 32 340896.0 33 440717.0 34 577889.0 35 791775.0 36 1102759.0 37 1146133.0 38 297860.0 39 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86439633897903 18.515986574022772 12.2864690045679 25.333148082430302 2 15.902041170140135 20.665391402819946 37.87939381215259 25.553173614887324 3 18.212671787099197 26.037447063067514 28.685903716993817 27.063977432839476 4 13.12215755005271 17.357266766372227 36.218508563217874 33.302067120357194 5 14.420455429237 37.47518876824704 33.34578826170904 14.758567540806924 6 31.90556937679619 36.64465224035505 17.49582071196287 13.953957670885888 7 27.89568917805884 31.82064720809647 21.958739642375807 18.32492397146888 8 26.828777600706736 33.71729861764198 19.988592929737393 19.46533085191388 9 26.96284372021724 15.67600723646586 19.11354369044697 38.247605352869925 10-11 25.295754745762267 25.907551676487888 26.929961765861744 21.8667318118881 12-13 26.125429523555933 23.715555985895605 26.445771582417997 23.713242908130468 14-15 24.12565411389939 24.597034176457015 23.761585080109022 27.51572662953457 16-17 24.10054229048105 27.01708267270947 24.646329287927905 24.236045748881576 18-19 24.50902491066229 26.523766804564858 25.695739838877824 23.27146844589503 20-21 25.604062158850294 25.956394929309823 24.79229587807007 23.647247033769816 22-23 25.12654867521859 25.714231263146015 24.55381270557844 24.605407356056958 24-25 24.625783446566253 26.151029293883514 24.53933814514004 24.68384911441019 26-27 24.757834076929182 26.070193593643243 24.435440569494236 24.73653175993334 28-29 24.4706335122454 26.013192933316652 24.969867953858085 24.546305600579863 30-31 24.90874449064483 25.95700226525918 24.801640927716505 24.332612316379485 32-33 24.39965803098854 26.14638194864701 24.56379547179236 24.890164548572088 34-35 24.590921941713432 26.13413483044354 25.012485609561715 24.262457618281314 36-37 24.812139335765725 25.970337745367715 24.621806138168775 24.595716780697778 38-39 24.851320222902594 25.81342984213027 24.757017763141658 24.57823217182548 40-41 24.627511603603075 25.894810956773817 25.000305474260127 24.477371965362984 42-43 24.975966226842854 25.822393067576282 24.73911733076727 24.46252337481359 44-45 24.697686463823043 26.02327061134344 24.66737057922167 24.611672345611844 46-47 24.386194253366533 26.02721566977802 25.094031901184604 24.492558175670844 48-49 24.977589657699074 25.97489503737234 24.88809429672638 24.1594210082022 50-51 24.241353275121753 26.294281624929155 24.835242019922575 24.629123080026513 52-53 24.34877277071553 26.094497849869487 24.53889903819516 25.01783034121982 54-55 24.471762121856464 25.89850658365623 24.626000404826502 25.0037308896608 56-57 24.645832375950462 25.56674888760435 24.607854694408765 25.17956404203642 58-59 24.405266986460138 25.645636027161185 25.168020673508963 24.78107631286972 60-61 24.538052691486854 25.788483416066267 24.865564749469684 24.8078991429772 62-63 24.189710654174007 25.87570482537621 24.740975833735178 25.193608686714608 64-65 24.06858591971413 25.65449218562385 24.935058547784404 25.341863346877613 66-67 24.694677360554138 25.589658237832435 24.798844091260353 24.916820310353074 68-69 24.559871412644238 25.257946260247998 24.927217749186415 25.254964577921353 70-71 24.06021283529905 25.469629422884378 24.922613763972308 25.54754397784426 72-73 24.63425096682115 25.535367318263596 24.856655949996114 24.973725764919138 74-75 24.818155242843403 25.35389911492945 24.864010276691747 24.9639353655354 76-77 24.4147011208128 25.47974647134662 25.07049314733527 25.03505926050531 78-79 24.570989361071007 25.53446701304945 24.95511399247704 24.939429633402504 80-81 24.26993290238932 25.444306308884467 25.224495046561962 25.061265742164245 82-83 24.32998833085055 25.556551572299735 24.976913373567626 25.136546723282088 84-85 24.460518397538937 25.643874094562563 24.66168018234816 25.233927325550344 86-87 24.26656703282922 25.355018549937284 24.9288671881664 25.4495472290671 88-89 24.367145391205906 24.96443359135005 25.132619497408008 25.535801520036035 90-91 24.612104083194996 25.270719865782393 24.925677585423323 25.19149846559928 92-93 24.35949730814966 25.322589395728052 24.938588249795473 25.379325046326812 94-95 23.852910885452697 25.37587464579602 25.392703993596548 25.378510475154737 96-97 24.50346388731861 25.165839926200462 24.96765429798991 25.36304188849102 98-99 24.36854277493971 25.20115102853733 24.727401527870192 25.702904668652778 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1531.0 1 1345.0 2 1152.5 3 1082.0 4 953.0 5 804.0 6 677.0 7 678.0 8 696.0 9 663.5 10 649.5 11 621.5 12 618.5 13 648.5 14 662.5 15 770.0 16 926.0 17 1083.0 18 1389.5 19 1785.0 20 2321.0 21 3344.5 22 5324.5 23 7538.0 24 9724.5 25 12963.5 26 18140.5 27 23647.5 28 29283.5 29 36073.0 30 42202.5 31 50126.5 32 59236.0 33 68315.5 34 76767.0 35 85492.0 36 94530.5 37 104831.0 38 118729.0 39 125965.5 40 131979.0 41 140578.5 42 152101.0 43 169515.5 44 188591.5 45 210537.5 46 237010.0 47 261196.5 48 277285.5 49 294884.0 50 317017.0 51 313218.0 52 298985.0 53 297420.5 54 286051.5 55 272616.0 56 250458.5 57 229931.0 58 217424.0 59 193244.0 60 163847.5 61 130584.0 62 104690.0 63 84327.5 64 63089.0 65 47262.5 66 35315.0 67 27564.5 68 22009.5 69 18705.5 70 15936.0 71 13254.5 72 11993.0 73 10325.5 74 7571.0 75 5597.0 76 4028.5 77 2651.5 78 1749.0 79 1095.5 80 698.0 81 487.5 82 327.5 83 217.0 84 150.0 85 113.0 86 86.5 87 54.0 88 37.0 89 30.5 90 18.5 91 17.5 92 17.5 93 12.0 94 10.0 95 12.0 96 8.5 97 8.0 98 7.0 99 7.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0019211584247177164 3 0.006147706959096692 4 0.0 5 0.005609782600175733 6 0.0 7 7.9920190468257E-4 8 0.0 9 0.0 10-11 3.073853479548346E-5 12-13 1.3063877288080472E-4 14-15 0.0026127754576160942 16-17 2.30539010966126E-4 18-19 0.0 20-21 0.0 22-23 3.8423168494354334E-5 24-25 9.913177471543417E-4 26-27 0.002405290347746581 28-29 0.0019826354943086833 30-31 0.002635829358712707 32-33 8.222558057791827E-4 34-35 7.684633698870865E-6 36-37 9.990023808532125E-5 38-39 0.06162307763124547 40-41 0.0032967078568156014 42-43 3.381238827503181E-4 44-45 1.536926739774173E-5 46-47 8.529943405746662E-4 48-49 1.3063877288080472E-4 50-51 8.222558057791827E-4 52-53 3.919163186424141E-4 54-55 0.0 56-57 5.840321611141859E-4 58-59 0.0 60-61 1.998004761706425E-4 62-63 5.379243589209606E-5 64-65 8.222558057791827E-4 66-67 0.0014600804027854644 68-69 0.0014908189375809479 70-71 6.91617032898378E-5 72-73 0.0017982042855357828 74-75 0.0017982042855357828 76-77 1.15269505483063E-4 78-79 1.383234065796756E-4 80-81 0.0 82-83 2.8433144685822205E-4 84-85 5.609782600175732E-4 86-87 0.0 88-89 7.684633698870865E-6 90-91 6.455092307051527E-4 92-93 0.0 94-95 0.0 96-97 0.003335131025309956 98-99 0.004764472893299937 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6506491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.8402491660676 #Duplication Level Percentage of deduplicated Percentage of total 1 74.88406584131944 32.08052039210336 2 13.341627444403374 11.431172879981725 3 4.698313797074836 6.038308011811775 4 2.14113085343944 3.6690631703400234 5 1.14281271381651 2.44791907050246 6 0.7601525254344335 1.9539074156296001 7 0.49642500402254763 1.4886879605214438 8 0.3622424802760876 1.2414846490849536 9 0.2694610080876672 1.0389399054313877 >10 1.5922299344974984 12.80120359965062 >50 0.1587864679976081 4.756982816252338 >100 0.1314908451854898 11.484428681532199 >500 0.014861094255570525 4.3427417613662875 >1k 0.006255357643106561 4.528105741000127 >5k 7.231627332595066E-5 0.18718400989328948 >10k+ 7.231627332595066E-5 0.5093499348983072 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 17325 0.2662725576658755 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 15557 0.2390996929066681 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6966 0.1070623166926689 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.536926739774173E-5 0.0 2 0.0 0.0 0.0 1.536926739774173E-5 0.0 3 0.0 0.0 0.0 1.536926739774173E-5 0.0 4 0.0 0.0 0.0 1.536926739774173E-5 0.0 5 0.0 0.0 0.0 6.147706959096692E-5 0.0 6 1.536926739774173E-5 0.0 0.0 6.147706959096692E-5 0.0 7 1.536926739774173E-5 0.0 0.0 6.147706959096692E-5 0.0 8 3.073853479548346E-5 0.0 0.0 6.147706959096692E-5 0.0 9 3.073853479548346E-5 0.0 0.0 6.147706959096692E-5 0.0 10-11 3.073853479548346E-5 0.0 0.0 7.684633698870866E-5 0.0 12-13 3.073853479548346E-5 0.0 0.0 7.684633698870866E-5 0.0 14-15 3.073853479548346E-5 0.0 0.0 7.684633698870866E-5 0.0 16-17 3.073853479548346E-5 0.0 0.0 7.684633698870866E-5 0.0 18-19 3.073853479548346E-5 0.0 0.0 7.684633698870866E-5 7.684633698870865E-6 20-21 6.916170328983778E-5 0.0 0.0 1.8443120877290078E-4 1.536926739774173E-5 22-23 8.453097068757953E-5 0.0 0.0 7.530941024893449E-4 3.073853479548346E-5 24-25 9.221560438645039E-5 0.0 0.0 0.0020748510986951336 3.073853479548346E-5 26-27 9.221560438645039E-5 0.0 0.0 0.003734731977651241 3.073853479548346E-5 28-29 1.1526950548306298E-4 1.536926739774173E-5 0.0 0.009782538698662612 3.073853479548346E-5 30-31 1.383234065796756E-4 1.536926739774173E-5 0.0 0.028118074704168498 3.073853479548346E-5 32-33 1.6906194137515906E-4 1.536926739774173E-5 0.0 0.05990940431639727 3.073853479548346E-5 34-35 1.6906194137515906E-4 1.536926739774173E-5 0.0 0.10096840216946432 3.073853479548346E-5 36-37 1.6906194137515906E-4 1.536926739774173E-5 0.0 0.16322930439771607 3.073853479548346E-5 38-39 1.8443120877290078E-4 1.536926739774173E-5 0.0 0.2597636729229319 3.073853479548346E-5 40-41 1.8443120877290078E-4 1.536926739774173E-5 0.0 0.3759553344498594 3.842316849435433E-5 42-43 1.9980047617064253E-4 1.536926739774173E-5 0.0 0.4807660534687591 4.6107802193225195E-5 44-45 2.1516974356838425E-4 1.536926739774173E-5 0.0 0.6024829666251748 4.6107802193225195E-5 46-47 2.1516974356838425E-4 1.536926739774173E-5 0.0 0.7315156510629155 4.6107802193225195E-5 48-49 2.1516974356838425E-4 1.536926739774173E-5 0.0 0.8684250850419988 9.221560438645039E-5 50-51 2.1516974356838425E-4 3.073853479548346E-5 0.0 1.016584822756229 9.221560438645039E-5 52-53 2.1516974356838425E-4 3.073853479548346E-5 0.0 1.1621702081813376 9.221560438645039E-5 54-55 2.2285437726725512E-4 3.073853479548346E-5 0.0 1.3070024995039569 9.221560438645039E-5 56-57 2.30539010966126E-4 3.073853479548346E-5 0.0 1.4709234209345714 9.221560438645039E-5 58-59 2.30539010966126E-4 3.073853479548346E-5 0.0 1.6506208953489678 9.221560438645039E-5 60-61 2.30539010966126E-4 3.073853479548346E-5 0.0 1.830203100257881 9.221560438645039E-5 62-63 2.30539010966126E-4 3.073853479548346E-5 0.0 2.013381713737866 1.0758487178419212E-4 64-65 2.30539010966126E-4 3.073853479548346E-5 0.0 2.20193957080706 1.0758487178419212E-4 66-67 2.30539010966126E-4 3.073853479548346E-5 0.0 2.4057898489370073 1.0758487178419212E-4 68-69 2.30539010966126E-4 3.073853479548346E-5 0.0 2.6262389358565166 1.0758487178419212E-4 70-71 2.30539010966126E-4 3.073853479548346E-5 0.0 2.8741759575168855 1.0758487178419212E-4 72-73 2.30539010966126E-4 3.073853479548346E-5 0.0 3.1171717597088815 1.0758487178419212E-4 74-75 2.3822364466499684E-4 3.073853479548346E-5 0.0 3.361497003530782 1.0758487178419212E-4 76-77 2.5359291206273856E-4 4.6107802193225195E-5 0.0 3.6226900183217037 1.0758487178419212E-4 78-79 2.6127754576160943E-4 4.6107802193225195E-5 0.0 3.8965473094483647 1.0758487178419212E-4 80-81 2.6127754576160943E-4 4.6107802193225195E-5 0.0 4.191399019840341 1.0758487178419212E-4 82-83 2.6127754576160943E-4 4.6107802193225195E-5 0.0 4.5086130142960315 1.2295413918193384E-4 84-85 2.6127754576160943E-4 4.6107802193225195E-5 0.0 4.824336189814141 1.2295413918193384E-4 86-87 2.766468131593512E-4 4.6107802193225195E-5 0.0 5.15108681469013 1.2295413918193384E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2230 0.0 25.02043 1 GTATAAG 2645 0.0 19.512625 1 TAGGCAT 3265 0.0 19.08256 5 TAGTACT 3135 0.0 18.983988 4 CTAGTAC 3270 0.0 17.916006 3 TACTAGG 3260 0.0 17.828337 2 GTACAAG 3320 0.0 17.786236 1 GTGATCG 1715 0.0 17.622097 8 GTATATA 1900 0.0 17.61965 1 CTAACGC 1050 0.0 17.270056 3 CATGGGG 14675 0.0 17.172606 4 CTAGGCA 3840 0.0 17.07273 4 GTATAAT 2045 0.0 16.5977 1 GTACATG 45540 0.0 16.407455 1 TACATGG 44650 0.0 16.328377 2 ATAAGGT 2325 0.0 16.19871 3 CCTAGTA 3515 0.0 16.005838 2 TCTAACG 1085 0.0 15.855882 2 GTATCAA 31560 0.0 15.73449 1 ACATGGG 43265 0.0 15.539963 3 >>END_MODULE