FastQCFastQC Report
Fri 27 May 2016
ERR765238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6506491
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT172730.26547335576119296No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA170900.2626607798274062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150330.23104619679025143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94850.14577750126758032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82190.1263200087420393No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG69030.10609405284661118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5200.024.145562
GTATCAA383450.024.023721
AGTACTT221150.021.0389312-13
CAACGCA450650.020.2563465
ATCAACG453150.020.124073
AACGCAG472150.019.6782116
TAGTACT34050.019.666434
TCAACGC471600.019.45514
GTCCTAC21050.019.4497661
TATCAAC477600.019.3176442
GAGTACT209450.018.88371712-13
CTAGTAC35600.018.2876633
TACTTTT257900.018.28416414-15
GTATAAG26550.018.2243751
ACGCAGA498000.018.1712027
CGCAGAG502400.018.0124748
TAACGCA4153.6379788E-1217.9288354
CATGGGG129600.017.5103574
ATACGGG16800.017.4385913
ATTGCGT11950.017.1220176
Produced by FastQC (version 0.11.5)