FastQCFastQC Report
Fri 27 May 2016
ERR765237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12953354
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT534170.41237968174111506No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA525830.4059411948442079No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG333570.2575163158514775No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG249030.1922513659396632No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG194430.15010012078724938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192790.14883403943102305No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC181240.1399174298795509No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT176170.13600338568682674No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC170550.1316647410392706No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC152030.11736728572383646No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA149060.11507444326774362No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC143600.11085931875250225No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT139870.10797975566791426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC90900.020.7315561
TCTACAC114850.020.5415953
GTACTAG39600.020.2932851
GTATAAG43150.019.6039281
TACACTC108300.019.2731865
CTACACT136400.019.1932814
CTAACGC22400.019.092153
TCTAACG24050.018.7581122
CATGGGG271550.018.2604334
ATCTACA118500.016.5764262
CGTTAAC15950.016.4998021
GTATTAC24700.015.601571
TAGTACT44200.015.3125894
ATGGGGG162150.015.278925
GTAGGAC43150.015.2478533
GTATATA30100.014.9884311
GTGATCG32000.014.9858978
TAACGCC44650.014.9477324
ACGCCTA28550.014.8203076
TAGGCAT61300.014.7244725
Produced by FastQC (version 0.11.5)