##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765237_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12953354
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08897965731501	33.0	33.0	33.0	33.0	33.0
2	32.07810123926205	33.0	33.0	33.0	33.0	33.0
3	32.07292088211285	33.0	33.0	33.0	33.0	33.0
4	35.85296734729862	37.0	37.0	37.0	33.0	37.0
5	35.852064183531155	37.0	37.0	37.0	33.0	37.0
6	35.79180658538321	37.0	37.0	37.0	33.0	37.0
7	35.732130689858394	37.0	37.0	37.0	33.0	37.0
8	35.72187118486841	37.0	37.0	37.0	33.0	37.0
9	35.735026001759856	37.0	37.0	37.0	33.0	37.0
10-11	35.69471860338257	37.0	37.0	37.0	33.0	37.0
12-13	35.67428103948985	37.0	37.0	37.0	33.0	37.0
14-15	37.300993279424006	40.0	37.0	40.0	33.0	40.0
16-17	37.3368972236843	40.0	37.0	40.0	33.0	40.0
18-19	37.377457799732795	40.0	37.0	40.0	33.0	40.0
20-21	37.38533969657588	40.0	37.0	40.0	33.0	40.0
22-23	37.36265124847202	40.0	37.0	40.0	33.0	40.0
24-25	37.33194827378299	40.0	37.0	40.0	33.0	40.0
26-27	37.306929849983256	40.0	37.0	40.0	33.0	40.0
28-29	37.277798939178226	40.0	37.0	40.0	33.0	40.0
30-31	37.19314036349196	40.0	37.0	40.0	33.0	40.0
32-33	37.08463915986547	38.5	37.0	40.0	33.0	40.0
34-35	36.979546069689746	37.0	37.0	40.0	33.0	40.0
36-37	36.91707410296978	37.0	37.0	40.0	33.0	40.0
38-39	36.823631740474326	37.0	37.0	40.0	33.0	40.0
40-41	36.68371280519315	37.0	37.0	40.0	33.0	40.0
42-43	36.57518655013983	37.0	37.0	40.0	33.0	40.0
44-45	36.43433553966023	37.0	37.0	40.0	33.0	40.0
46-47	36.228891760388855	37.0	37.0	40.0	33.0	40.0
48-49	36.013886635075366	37.0	37.0	40.0	33.0	40.0
50-51	35.807662826168425	37.0	37.0	40.0	27.0	40.0
52-53	35.63933545705614	37.0	37.0	40.0	27.0	40.0
54-55	35.51556948107803	37.0	35.0	40.0	27.0	40.0
56-57	35.36219727338572	37.0	33.0	40.0	27.0	40.0
58-59	35.195678393410695	37.0	33.0	40.0	27.0	40.0
60-61	34.924110581707254	37.0	33.0	38.5	27.0	40.0
62-63	34.70921558231173	37.0	33.0	37.0	27.0	40.0
64-65	34.483635049269864	37.0	33.0	37.0	27.0	40.0
66-67	34.25217221732688	37.0	33.0	37.0	27.0	40.0
68-69	34.01876656810275	37.0	33.0	37.0	27.0	40.0
70-71	33.79309146495957	37.0	33.0	37.0	27.0	38.5
72-73	33.536300366684955	37.0	33.0	37.0	27.0	37.0
74-75	33.348681932108086	37.0	33.0	37.0	27.0	37.0
76-77	33.137285138659834	37.0	33.0	37.0	27.0	37.0
78-79	32.938073606264446	37.0	33.0	37.0	27.0	37.0
80-81	32.7585749605855	33.0	33.0	37.0	27.0	37.0
82-83	32.59501052777527	33.0	33.0	37.0	27.0	37.0
84-85	32.4431754123295	33.0	33.0	37.0	27.0	37.0
86-87	32.30365455927476	33.0	33.0	37.0	22.0	37.0
88-89	32.182359989544025	33.0	33.0	37.0	22.0	37.0
90-91	32.063553810078844	33.0	33.0	37.0	22.0	37.0
92-93	31.906558602505577	33.0	33.0	37.0	22.0	37.0
94-95	31.81906168857888	33.0	33.0	37.0	22.0	37.0
96-97	31.649149363168796	33.0	33.0	37.0	22.0	37.0
98-99	31.530507040879144	33.0	33.0	37.0	22.0	37.0
100	31.44031406846443	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	7.0
7	332.0
8	1752.0
9	2411.0
10	2612.0
11	4820.0
12	10904.0
13	20099.0
14	25323.0
15	26251.0
16	28153.0
17	32957.0
18	40723.0
19	48519.0
20	59381.0
21	75990.0
22	88476.0
23	87778.0
24	86641.0
25	91660.0
26	100537.0
27	117482.0
28	147161.0
29	190578.0
30	250493.0
31	336677.0
32	460902.0
33	646883.0
34	940748.0
35	1504370.0
36	2675554.0
37	3724491.0
38	1122256.0
39	430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.344557598254404	18.7575257630701	12.956891131888664	24.941025506786836
2	15.816475148171708	20.97029277813622	37.48917697448464	25.724055099207433
3	18.37074311592418	25.676996338365143	29.501700800284862	26.45055974542581
4	13.206204215146187	17.30008227888024	36.223784657092715	33.269928848880866
5	14.116565691348292	37.08455289951135	33.52947534784177	15.269406061298593
6	31.82253895970944	36.68295630255889	17.115814169373714	14.37869056835795
7	27.087997409567844	32.2089803655403	21.800302479586797	18.902719745305056
8	26.762937758121296	33.08014425917239	20.15890837139881	19.998009611307506
9	26.963401534670993	14.791966914760668	19.59934631838544	38.6452852321829
10-11	25.071667727231013	25.753373596554063	26.992552394265996	22.18240628194893
12-13	26.311265858228982	23.60400942547507	26.722657388429248	23.362067327866697
14-15	24.237313110804344	24.015954353307183	24.727398465953698	27.019334069934775
16-17	23.460231994171792	26.81397560702481	25.735531091047513	23.990261307755883
18-19	23.992814163671763	26.222361369895115	27.339312265200366	22.445512201232756
20-21	24.960710307915228	25.443010240834983	26.410092129763406	23.186187321486376
22-23	24.428678372833946	25.449542253300944	25.82660707741623	24.29517229644888
24-25	24.071954926803382	25.896853184037393	25.796454713009336	24.23473717614989
26-27	24.264623373411	25.505048331325646	25.895347472271496	24.33498082299186
28-29	24.010345249662986	25.294063629029186	26.787768867618457	23.90782225368937
30-31	24.672800807328475	25.18865534688896	26.345964127906598	23.792579717875963
32-33	23.692274465468046	25.749556652003648	26.1618887240446	24.39628015848371
34-35	23.951574066728778	25.725745293858665	26.492026644570306	23.83065399484225
36-37	24.329364659631093	25.564835353185195	26.060810190028466	24.044989797155246
38-39	24.50652241517587	24.94850054398496	26.212582311888212	24.332394728950963
40-41	24.13451296439731	25.277029781336076	26.346550913351365	24.24190634091525
42-43	24.798082090115994	25.170324477998108	26.071585555627312	23.960007876258587
44-45	24.431355095073634	25.28099403567785	26.096173391889277	24.19147747735924
46-47	23.97319202230879	25.27399754796043	26.498990438836945	24.253819990893835
48-49	24.538846170818243	25.35322308013545	26.154651310887694	23.95327943815861
50-51	23.864784543249044	25.659177223912224	25.722115750528417	24.753922482310315
52-53	23.58694640382967	25.894410060907397	25.269885016182503	25.24875851908043
54-55	23.909396708439846	25.574238831878425	25.238342180369894	25.278022279311834
56-57	24.179702886416	24.981215512953835	25.410798577447892	25.428283023182274
58-59	23.81593760160111	24.91403836581519	26.169091736341237	25.100932296242455
60-61	23.991615192605167	25.27626601600936	25.683122423152362	25.048996368233112
62-63	23.402848421230626	25.31496052352702	25.683909481495608	25.598281573746746
64-65	23.11255742326839	25.313091225485636	25.783147394814097	25.791203956431875
66-67	24.016924005091006	25.191228386006514	25.4053226406788	25.38652496822367
68-69	23.952904329033842	24.825925085540653	25.522271066077558	25.69889951934795
70-71	23.52930322459808	25.14463253203803	25.531039857240057	25.79502438612383
72-73	24.247633234843743	25.053912685499903	25.50687884251659	25.19157523713977
74-75	24.21207914602106	25.10586223846532	25.499920133832383	25.182138481681232
76-77	23.781067312486257	25.266079203490882	25.696969603384183	25.255883880638674
78-79	23.777224461555697	25.38190541179962	25.641844973342494	25.199025153302195
80-81	23.56965587525116	25.432886119780658	25.690023296795772	25.307434708172412
82-83	23.702626342042464	25.24806588934504	25.508254349090347	25.541053419522154
84-85	23.89510993379324	25.350712840706358	25.138702975993898	25.615474249506505
86-87	23.682212502844433	25.065991813050832	25.460466191729168	25.79132949237557
88-89	23.778694480463034	24.642836960291163	25.701248217813248	25.87722034143255
90-91	23.872802136614066	24.888716730724173	25.506610140115903	25.731870992545858
92-93	23.69637514544036	25.082693964453245	25.44117697579546	25.779753914310938
94-95	23.138316947035502	25.070858118781313	25.802681032042234	25.988143902140948
96-97	23.596077293494787	25.066163894537514	25.33781771610145	25.999941095866248
98-99	23.546913625997277	25.111242872164503	25.266657865440052	26.075185636398167
100	23.483168222656836	25.123188345375773	25.297602227951867	26.096041204015524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	861.0
1	829.0
2	832.5
3	832.0
4	655.5
5	567.5
6	759.5
7	1420.0
8	2260.5
9	2277.0
10	1664.5
11	1417.5
12	1616.5
13	1963.5
14	2378.5
15	2988.5
16	3705.0
17	4511.5
18	5398.5
19	6491.0
20	7962.5
21	9715.0
22	12233.0
23	15425.5
24	18980.0
25	24086.0
26	31635.0
27	40461.5
28	50042.0
29	65251.5
30	82999.0
31	98129.0
32	118044.5
33	139630.5
34	161159.0
35	180086.5
36	200055.0
37	230248.5
38	249124.5
39	256510.0
40	264658.5
41	274304.0
42	291469.0
43	322478.0
44	359450.0
45	394756.0
46	440141.0
47	492323.5
48	559324.5
49	621880.5
50	685644.0
51	688695.0
52	622423.0
53	588440.0
54	555224.5
55	510174.5
56	477993.5
57	432351.5
58	388841.0
59	354682.0
60	305714.0
61	252655.5
62	204849.5
63	164737.5
64	129999.5
65	102113.0
66	82066.5
67	64302.0
68	51033.0
69	42520.5
70	35477.0
71	30279.5
72	25726.0
73	23829.5
74	19495.0
75	15101.0
76	11931.0
77	8914.5
78	6586.5
79	4379.0
80	3091.0
81	2311.5
82	1809.5
83	1435.0
84	1010.0
85	676.5
86	475.0
87	287.0
88	166.0
89	110.5
90	76.5
91	53.0
92	33.5
93	26.0
94	20.5
95	9.5
96	5.0
97	5.5
98	3.0
99	1.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02192482348587092
2	0.004979405333938994
3	0.009387530055922197
4	0.008206368790662248
5	0.010375691114440321
6	0.005728246136097261
7	0.008299008889898324
8	0.008569209179336873
9	0.006724127202885059
10-11	0.026923528840484098
12-13	0.029088991160127333
14-15	0.011800032640194963
16-17	0.0037480640149261733
18-19	0.011475792292868705
20-21	0.0016289217449009731
22-23	8.492009096640144E-5
24-25	0.003609103866072061
26-27	0.0016405017573054824
28-29	0.0012545013438218396
30-31	0.026884928799135732
32-33	0.02121844272919585
34-35	0.007692988240729004
36-37	0.008368488964325379
38-39	0.006098806533041558
40-41	0.014695035741322287
42-43	0.0038870241637802842
44-45	0.0038522841265667563
46-47	0.013023653950938113
48-49	0.017180878404156944
50-51	0.007974768542572062
52-53	0.021558123093061456
54-55	0.027309529253967738
56-57	0.014224115236872241
58-59	0.010460611205406723
60-61	0.008634829249629092
62-63	0.015200696282985858
64-65	0.009098029745809463
66-67	0.017620918875528302
68-69	0.005720526127827588
70-71	0.005411725797040674
72-73	0.010753971519654291
74-75	0.003358203597307693
76-77	0.010317791052417775
78-79	0.0026479628364977905
80-81	0.007171887682526086
82-83	0.0018759620095305048
84-85	0.008696589315786476
86-87	0.00325398348566711
88-89	0.0012815213727656945
90-91	0.001150281232181256
92-93	1.582601695282936E-4
94-95	0.0027560429522732106
96-97	7.990208559111409E-4
98-99	0.004740085077579135
100	0.006407606863828473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2953354E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.284121746844406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61830738901956	28.1841224287784
2	13.842373679156692	10.598862383962986
3	4.911801862640662	5.641320615171367
4	2.264883907382316	3.46836365010773
5	1.281122448179069	2.452332388935142
6	0.8168564256038008	1.876357850850484
7	0.5410584432583755	1.4499763119703226
8	0.4073439383131426	1.2475843941775542
9	0.291428541425483	1.0041377184394633
>10	1.6998412060312285	12.198489840160853
>50	0.16171406397704682	4.325196761947173
>100	0.13029713980184268	10.186035643284876
>500	0.02012971664358019	5.412444793239303
>1k	0.0121536784515865	8.40386721560849
>5k	3.842247707322023E-4	0.9534832661340701
>10k+	3.0333534531489653E-4	2.59742473723169
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	53417	0.41237968174111506	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	52583	0.4059411948442079	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	33357	0.2575163158514775	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	24903	0.1922513659396632	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	19443	0.15010012078724938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19279	0.14883403943102305	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18124	0.1399174298795509	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	17617	0.13600338568682674	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	17055	0.1316647410392706	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15203	0.11736728572383646	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	14906	0.11507444326774362	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	14360	0.11085931875250225	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	13987	0.10797975566791426	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.172405540680815E-4	0.0	0.0	1.5440016539345717E-5	7.720008269672858E-6
2	8.800809427427058E-4	7.720008269672858E-6	0.0	2.3160024809018577E-5	7.720008269672858E-6
3	8.878009510123788E-4	7.720008269672858E-6	0.0	2.3160024809018577E-5	7.720008269672858E-6
4	0.0010036010750574715	7.720008269672858E-6	0.0	2.3160024809018577E-5	7.720008269672858E-6
5	0.0034662837130831135	1.5440016539345717E-5	0.0	1.3896014885411145E-4	7.720008269672858E-6
6	0.00726452778176216	1.5440016539345717E-5	0.0	2.3160024809018576E-4	1.5440016539345717E-5
7	0.008430249030482761	1.5440016539345717E-5	0.0	2.3160024809018576E-4	1.5440016539345717E-5
8	0.010406571147519013	1.5440016539345717E-5	0.0	2.4704026462953147E-4	3.0880033078691434E-5
9	0.011572292396239615	2.3160024809018577E-5	0.0	2.624802811688772E-4	3.0880033078691434E-5
10-11	0.022932284565063226	2.3160024809018577E-5	0.0	4.554804879106987E-4	3.0880033078691434E-5
12-13	0.043872806996550856	2.3160024809018577E-5	0.0	8.955209592820516E-4	5.018005375287358E-5
14-15	0.06994713492737094	3.474003721352786E-5	0.0	0.0014513615546984974	6.176006615738287E-5
16-17	0.0817896276130491	3.860004134836429E-5	0.0	0.0017524418772157388	9.650010337091074E-5
18-19	0.08855621486141736	4.6320049618037154E-5	0.0	0.0019300020674182146	1.0036010750574716E-4
20-21	0.11295916100185327	5.404005788771001E-5	0.0	0.002555322737261716	1.1580012404509288E-4
22-23	0.11969872822127767	5.404005788771001E-5	0.0	0.003300303535285147	1.428201529889479E-4
24-25	0.15293336382221934	5.790006202254644E-5	0.0	0.006774307256637933	1.5440016539345716E-4
26-27	0.23872195571895896	6.176006615738287E-5	0.0	0.017582318834179933	1.6598017779796643E-4
28-29	0.25557087376751997	6.56200702922193E-5	0.0	0.03485197733343812	1.6984018193280288E-4
30-31	0.2612373598374599	8.878009510123787E-5	0.0	0.08596615208694211	1.8142019433731217E-4
32-33	0.26618974514245497	9.264009923607431E-5	0.0	0.18111139400652526	2.0072021501149433E-4
34-35	0.26940898859090856	1.0808011577542002E-4	0.0	0.31142513359860313	2.1616023155084004E-4
36-37	0.27564289526866936	1.2352013231476573E-4	0.0	0.5165187332948671	2.1616023155084004E-4
38-39	0.28202734210768887	1.312401405844386E-4	0.0	0.8343398937448943	2.393202563598586E-4
40-41	0.2880373685456292	1.5440016539345716E-4	0.0	1.2473448961558526	2.393202563598586E-4
42-43	0.3029099644771539	1.6212017366313002E-4	0.0	1.5834779162215438	2.509002687643679E-4
44-45	0.32041122322450233	1.6598017779796643E-4	0.0	1.9497035285224198	2.740602935733865E-4
46-47	0.3268226900924656	1.6984018193280288E-4	0.0	2.383629753344192	2.933603142475686E-4
48-49	0.33157435518244927	1.6984018193280288E-4	0.0	2.812800453071845	3.088003307869143E-4
50-51	0.3409117051846186	1.7756019020247573E-4	0.0	3.27662626992206	3.203803431914236E-4
52-53	0.34349018794668934	1.7756019020247573E-4	0.0	3.7725943412030585	3.7828040521397006E-4
54-55	0.35261523772144265	1.8914020260698503E-4	0.0	4.237639919359882	3.821404093488065E-4
56-57	0.37279919934250233	1.9300020674182147E-4	0.0	4.7522788306410835	3.9372042175331583E-4
58-59	0.37840392534628486	1.9300020674182147E-4	0.0	5.300241157618328	4.091604382926615E-4
60-61	0.3822253294397729	1.9300020674182147E-4	0.0	5.837391613013896	4.1688044656233437E-4
62-63	0.385668453128047	1.9300020674182147E-4	0.0	6.385338499974601	4.246004548320072E-4
64-65	0.3882353558777132	1.9686021087665791E-4	0.0	6.956931772265314	4.246004548320072E-4
66-67	0.39149705937165	2.0844022328116719E-4	0.0	7.557787735902222	4.2846045896684367E-4
68-69	0.3945348826257663	2.1616023155084004E-4	0.0	8.226463972188206	4.323204631016801E-4
70-71	0.3981555665042429	2.2002023568567646E-4	0.0	8.993821214181285	4.4776047964102574E-4
72-73	0.40868951778821144	2.238802398205129E-4	0.0	9.70094309165024	4.8250051685455364E-4
74-75	0.41923118908044976	2.2774024395534934E-4	0.0	10.391605911488252	4.940805292590629E-4
76-77	0.42243113250822917	2.393202563598586E-4	0.0	11.139682432827822	5.018005375287358E-4
78-79	0.4246622148981646	2.393202563598586E-4	0.0	11.915778724182168	5.018005375287358E-4
80-81	0.42959916018662037	2.393202563598586E-4	0.0	12.76282961154308	5.249605623377544E-4
82-83	0.43101578170410537	2.393202563598586E-4	0.0	13.673732687302454	5.597005995512822E-4
84-85	0.4370489681668547	2.393202563598586E-4	0.0	14.545236700857554	5.635606036861186E-4
86-87	0.4485286204638582	2.393202563598586E-4	0.0	15.417493415218946	5.712806119557916E-4
88	0.450894802998513	2.393202563598586E-4	0.0	16.09266603846386	5.867206284951372E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	9090	0.0	20.731556	1
TCTACAC	11485	0.0	20.541595	3
GTACTAG	3960	0.0	20.293285	1
GTATAAG	4315	0.0	19.603928	1
TACACTC	10830	0.0	19.273186	5
CTACACT	13640	0.0	19.193281	4
CTAACGC	2240	0.0	19.09215	3
TCTAACG	2405	0.0	18.758112	2
CATGGGG	27155	0.0	18.260433	4
ATCTACA	11850	0.0	16.576426	2
CGTTAAC	1595	0.0	16.499802	1
GTATTAC	2470	0.0	15.60157	1
TAGTACT	4420	0.0	15.312589	4
ATGGGGG	16215	0.0	15.27892	5
GTAGGAC	4315	0.0	15.247853	3
GTATATA	3010	0.0	14.988431	1
GTGATCG	3200	0.0	14.985897	8
TAACGCC	4465	0.0	14.947732	4
ACGCCTA	2855	0.0	14.820307	6
TAGGCAT	6130	0.0	14.724472	5
>>END_MODULE