FastQCFastQC Report
Thu 26 May 2016
ERR580299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG21.1627906976744187No Hit
TCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGAT21.1627906976744187No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC21.1627906976744187No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG10.5813953488372093No Hit
GCTCTGGACGATGCTGGATCTTTTCTTGTTTGAATGTTATGAGACTTCCA10.5813953488372093No Hit
CATGGACGCCCCGCCCCACCAAGCGCGGCGCACAAAACATTTGCTGCCGG10.5813953488372093No Hit
GAATTGGGGACGTTCAAATGCGCATCCTAGTCACGGCGCTTAAAATGAAC10.5813953488372093No Hit
TATCAACGCAGAGTACATGGGGGAATTCGATATGCGTTACGTGAGTCTGA10.5813953488372093No Hit
ACACAGGGCAGGGTGACACTGAGACCCCTGTTCATTCTTAAAAACAAAAA10.5813953488372093No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA10.5813953488372093No Hit
ACGCTCGCTCACCCACACAGTATTCCTCCCGACCTTTCCCCCACTGCCCG10.5813953488372093No Hit
CGGGCATGGGGCAAGTATTGAGGCGGGAGCGGGCGCGTCGGGGGGCCAGA10.5813953488372093No Hit
TCACGGCTCAGCACCCGGCCCCGGGCACGGCACCGCAACGGCCGGGGCGT10.5813953488372093No Hit
GGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGG10.5813953488372093No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10.5813953488372093No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTCACTTCA10.5813953488372093No Hit
GAATTGTAGCTGACGGCAGGCCCCTAAACCCAAAAGTCGGGTTCTACAGG10.5813953488372093No Hit
GCCTAAAACACCCCTCCGACCCGCACCTGCAGCCCGACCTCCGCCCGCTC10.5813953488372093No Hit
CGAGCGCCACCGCGTCTCCACGGCAACGCCCGCCCCACTCCTGCACTCCC10.5813953488372093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC10.5813953488372093No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT10.5813953488372093No Hit
GTACAATGCGGTTCTCCTCCTCGGGGGAAATAGGCCTGAATCGCTTCGTT10.5813953488372093No Hit
CACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCC10.5813953488372093No Hit
GGGTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACC10.5813953488372093No Hit
ATAATATAGAGAGTGAGATACAGGATGAGCTATTTCAGGGAGTTATTCAG10.5813953488372093No Hit
CAACAGTCCGACGGCCTCAACCCCCCCCTTTACCACAAAGCCCCTTGGCC10.5813953488372093No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA10.5813953488372093No Hit
GCTCCATTAGTAGCCAGAATAAGTGGGTTTTGCTTTCAATTTTCACGGAT10.5813953488372093No Hit
AATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTT10.5813953488372093No Hit
TGGAAGACGCATGGTGTAGAGGGGGGTGGGCGCCGGGACGGGTCCGGGCA10.5813953488372093No Hit
GAGGGGACGTCCCAGGGAGGGGTCGACGGGGACACGTGGCGGTCTTGAAT10.5813953488372093No Hit
GGGTCATCCTTTGTTGGTGCAAGAAATCTCCATGCCTCTAACACTCGACT10.5813953488372093No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT10.5813953488372093No Hit
TACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTT10.5813953488372093No Hit
CATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTG10.5813953488372093No Hit
AGTTGTAGGTAGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGC10.5813953488372093No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG10.5813953488372093No Hit
ATGGTGTCACTGGGCTCCACCTCTAGGGTGATGGTCTTGCCCTGTCTCTT10.5813953488372093No Hit
AATCAGGACGGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGG10.5813953488372093No Hit
GAATAAGATTGTTTTTTTTGTTGTTGTTTTTGCTTTTTTTTACAAGGTTT10.5813953488372093No Hit
GTGGAGCTATATAAGCCGCTGTTGCGTAAATCAACGGCATGATCCCTATG10.5813953488372093No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG10.5813953488372093No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT10.5813953488372093No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA10.5813953488372093No Hit
TTCTTGGAGAACTCGGCGAAGTTCACCGACGAGTCGGGATGCTTCTTCTT10.5813953488372093No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA10.5813953488372093No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC10.5813953488372093No Hit
GCCCAGAATGGTGTGTGTCAGCTGATCCGAAGGGAGTTTCAGACCAGTGT10.5813953488372093No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10.5813953488372093No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTGACAAGACGGCGCAGGT10.5813953488372093No Hit
ATCTTATGTTATTAACCATTTCCTTAAATTCTTCTGGGTCTGCTGACAAA10.5813953488372093No Hit
CAATGGCACTCTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAA10.5813953488372093No Hit
CCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAA10.5813953488372093No Hit
GGTTGTTGTCTCTGTTAATGTCCCAGAGGTTCCAATAACCAATTTAAATA10.5813953488372093No Hit
CCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCT10.5813953488372093No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA10.5813953488372093No Hit
GGCCCACATGGAATATCGGTTTGAGGCCCCGAGCTGGAGGGTTCTTATCT10.5813953488372093No Hit
CCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGAC10.5813953488372093No Hit
ATGATGCCTTATGCGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT10.5813953488372093No Hit
CTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCAT10.5813953488372093No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA10.5813953488372093No Hit
CTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTGAC10.5813953488372093No Hit
CCTTCGTAGGGCTACTGTGGAGTGTGTGAAAGATCAGTTTGGCTTTATTA10.5813953488372093No Hit
CTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGC10.5813953488372093No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG10.5813953488372093No Hit
CTATGAGCACCATGGCTGCTGCAGAAGCAGCGGCTGCTGTTGTTGCAGCT10.5813953488372093No Hit
CCTTTAGACATCACCAAAGCTGGTAAGGCCGAGGCAGCCAGGGCAGAACA10.5813953488372093No Hit
GGTTGATCCCTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCC10.5813953488372093No Hit
GCGCTGAACTATGTTGGGTAGGGTATGGAAAAAGAAAGAGGGGAGGGGTG10.5813953488372093No Hit
ATGTTACAACGATTATCTTACACATTAAAAAGCTTGCATTGAAAATCTAC10.5813953488372093No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA10.5813953488372093No Hit
TGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAG10.5813953488372093No Hit
GCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAA10.5813953488372093No Hit
CATCCAGAGTTTCCAACTGTTCTGACAGCTTTAGAAATAGACAATGCTGT10.5813953488372093No Hit
GCCTAGACACTTGACGAACTCCATCCTTGCGACTGCTGGGCGCGGGGCTC10.5813953488372093No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC10.5813953488372093No Hit
CTTCAATACAGAGCAGAGTTATTGTAGGCCATTCTACATTTCTTCAGTGT10.5813953488372093No Hit
GCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAGGA10.5813953488372093No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG10.5813953488372093No Hit
GTACATGGGGGGGAATTCGAGCACGCATTTTGAAAATTCTATGGAAGAGC10.5813953488372093No Hit
CCGCTGAACAGAGCCCCAGCGGGTTCCAGGAAGCTTAAGTGGGTTCCGAG10.5813953488372093No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT10.5813953488372093No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA10.5813953488372093No Hit
GCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGA10.5813953488372093No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA10.5813953488372093No Hit
GAATGAGTCACACAGCTATCGACAGAGTCGTGACCCTCATCGACAGTTCG10.5813953488372093No Hit
GATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCAT10.5813953488372093No Hit
TGTCCGCACCAAGACCCTGGTGAAGAACTGCATCGTGCTTATTGACAGCA10.5813953488372093No Hit
GCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGC10.5813953488372093No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGCA10.5813953488372093No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT10.5813953488372093No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA10.5813953488372093No Hit
GCGCGGCCAGCAAACCCCGGGGGGCGACTGCCGCTAGAGTGGCCGCGGCC10.5813953488372093No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA10.5813953488372093No Hit
GAAGTACCGTGCCCTGAGATTGGATGTGGGGAACTTTTCCTGGGGCTCTG10.5813953488372093No Hit
GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT10.5813953488372093No Hit
ACCCTGTAGAGAAGGCCCTTCGAGATGCCAAGCTGGACAAGTCACAGATC10.5813953488372093No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA10.5813953488372093No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT10.5813953488372093No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC10.5813953488372093No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10.5813953488372093No Hit
GCTTAAGCATGGTTGTTTATAAATTTAATGAGGAAAAATACATTTGCTCG10.5813953488372093No Hit
GAGGTGTGGATGCTCTCTATTTAGCGGAGAAATATGGTACACCTCTTTAC10.5813953488372093No Hit
CCTAAACCCAAAAGTCGGGTTCTACAGGGTATATGATGTTGCTCTGCAAG10.5813953488372093No Hit
AAGCAGCCTCAATATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCG10.5813953488372093No Hit
CGCTTGAAACGATGTGACATCGCTTCTAGAGTATGAGCCGCAATGCACGA10.5813953488372093No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG10.5813953488372093No Hit
ATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAA10.5813953488372093No Hit
CCGAGGGGCCCGTTAGGCCCTGTAATCTGTCGTCCATGAGCCGGGGCACT10.5813953488372093No Hit
GAATGCAGGACGCCCAACCGCCGGACGACGCCGAATCACAAGTACTCAAG10.5813953488372093No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT10.5813953488372093No Hit
CTCTTATTCCTGAAGGCAGTTAGCTTTTCATCATCCTTTCTGTCTAGATA10.5813953488372093No Hit
GAACATAGAAGTGTACTTTCTGTCTCTCCATGCTGAAACCCTTTCGGAAG10.5813953488372093No Hit
CGCGAAGAACGCTCCACTTGTTAGGGTTACAACACTTACTGGAAAAACCC10.5813953488372093No Hit
GTCTTATAGTAGATGCAGCGGCAGTCGAGTACATGCACCAGCTACAGTGC10.5813953488372093No Hit
GTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAA10.5813953488372093No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG10.5813953488372093No Hit
CCCCGGACCTTGGAGTTTCATGCTAATCAAAGAAGAGCTAATCCGACGTA10.5813953488372093No Hit
GTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTATT10.5813953488372093No Hit
ATATGTAGCGGCGTCAGCCCTGTTCCGTTTTTGTGTGACGCTGGTCAGTA10.5813953488372093No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA10.5813953488372093No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA10.5813953488372093No Hit
TTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCACTTTTTCTAAT10.5813953488372093No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC10.5813953488372093No Hit
TTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACTAGCCTATCGCAGACG10.5813953488372093No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT10.5813953488372093No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG10.5813953488372093No Hit
TGTCAGTACTATGTGTGAGTGCTCCGCCCTAGTGCTGATGTATGCCCCAT10.5813953488372093No Hit
ACTTGAGCGTGGATATGGTACAACTCTGGGTAACTCCTTACGTCGTATCC10.5813953488372093No Hit
GCCAGACCGTCACCTGAGAGGAAAGCCACATCAAGGATTTGAAATGGGCC10.5813953488372093No Hit
GGATTCAGTCGTGTCAAGCGCGTGTCATACGATTACAAGGCGCGGTCAGA10.5813953488372093No Hit
ATATGATACGCAGTCGCATCCTATCTTACGTGTCAGTACTATGTGTGAGT10.5813953488372093No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG10.5813953488372093No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA10.5813953488372093No Hit
GCTGCCAGGCTGGGCTGGTCCCAGCCCTTAGGTAACCTCCAAGCTCCCTT10.5813953488372093No Hit
ACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATG10.5813953488372093No Hit
TGGGCCACGCCAGGAAAAAACCTGACGGGAATATCATACCCTCCTAGGGT10.5813953488372093No Hit
GGGGAGGCCACCGCGCTTAAGTCCCCTTGGGACATACTGTTTGACCGCTG10.5813953488372093No Hit
CTGCGTGTGGAGTGTGACTGATCCTGTTTTTATTCTGGAAATTCCATTGG10.5813953488372093No Hit
GCATATGGCCCTACTTAACTGATCTGAAGTATTACGGTAACCGCGACGAT10.5813953488372093No Hit
TCCCTACACGCTATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC10.5813953488372093No Hit
GTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGT10.5813953488372093No Hit
AACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATT10.5813953488372093No Hit
CCCCAACACTGCCGAGCTCAAGATCTGCCGAGTAAACCGGAACTCTGGGA10.5813953488372093No Hit
ATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAA10.5813953488372093No Hit
TCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTT10.5813953488372093No Hit
CATTCTGATACTACAAAGTTACTTTTCTGGCTCATTGAACCATAACCCAA10.5813953488372093No Hit
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG10.5813953488372093No Hit
AGTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCATTTCAAGA10.5813953488372093No Hit
GGGAAGCTGATGTCCTCTACCATGCCCAAACCGCTGTCCTTGCCAACCAG10.5813953488372093No Hit
GGACAGGAGAAGACGGGCGGACAAAGCGAGCTCTCAGAACCGTGGGGAGA10.5813953488372093No Hit
TACGTGCGAACCCCGCGCCCCTGTCGATGTTACCCCCCATCGCGGAACAG10.5813953488372093No Hit
CCATGAACCTGGTGTCCGTATTCTGTGCCAGGGCCTGAAGGACTCTGCCT10.5813953488372093No Hit
AGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.5813953488372093No Hit
ATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTTG10.5813953488372093No Hit
GCCCAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10.5813953488372093No Hit
GTGGTGGGAGGCCCTCCACCTCCGCCTTGAGGCAGTTACCGCGAGGGCCC10.5813953488372093No Hit
CTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACG10.5813953488372093No Hit
CTTTTTAGGGTGGCATATCTGTATTATAAATCCTGGTCTTCCATCTGGTG10.5813953488372093No Hit
GAGTAGTTGAGAGAACGGAGCGGACGCCATGGCGGCCGACATCGAGCAGG10.5813953488372093No Hit
CCTATCATAACGGTGATAAATACCTGGCAATTGCTCCTCCTATCATTAAG10.5813953488372093No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG10.5813953488372093No Hit
GTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTT10.5813953488372093No Hit
CTGCACCGGAGGACCCACCAAAGAGGACAGCAAAACGACTACGACACACG10.5813953488372093No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT10.5813953488372093No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA10.5813953488372093No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA10.5813953488372093No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA10.5813953488372093No Hit
CAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGT10.5813953488372093No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)