FastQCFastQC Report
Thu 26 May 2016
ERR580299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG21.1627906976744187No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA21.1627906976744187No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA21.1627906976744187No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG21.1627906976744187No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA10.5813953488372093No Hit
TCACTGAATAAATCCCTGAAAAAACCCACCACTGAGGCATCTTTATCACT10.5813953488372093No Hit
CTATCGGAGTAGCCGCGCTGGTGCCACACGACTACAACCCTCGTAATAGG10.5813953488372093No Hit
CTAATGTGTCAGCGGCCTAACTGTAATTGATCCACACTGACGAATCATGT10.5813953488372093No Hit
TGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCG10.5813953488372093No Hit
GATCAATTACAGTTAGGCCGCTGACACATTAGTAACGTCGGCAAGCACTT10.5813953488372093No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG10.5813953488372093No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10.5813953488372093No Hit
GAGTACATGGGGCCTCAAACCGATATTCCATGTGGGCCAACTGCCATGTT10.5813953488372093No Hit
GATATTAATAGCCCCTAAGATAAACTATAATTGTTAAAATCTTAATGGAG10.5813953488372093No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10.5813953488372093No Hit
ACGCAGAGTACATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCC10.5813953488372093No Hit
CATCTGAGACCTCCATGGTCCTCACGGCCTGGCAGTGCCTCAGTCACTAG10.5813953488372093No Hit
GTGTGTCACTGTTCCAAGAGACAAAACAAATTTAAACAGCTAAACCATCT10.5813953488372093No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA10.5813953488372093No Hit
GTATCAACGCAGAGTACATGGGAGCGCTCGCAGCAGACAGACGCCGCCCC10.5813953488372093No Hit
ATCCACATCTCTGCTTAGAAACTTGGGTCTTGTACTTTCAGATGAATAAT10.5813953488372093No Hit
GACCTTCATCTTCCAGCTGAAAATAAGTACATAGCCACGGACTTTACGTT10.5813953488372093No Hit
GAGTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG10.5813953488372093No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT10.5813953488372093No Hit
GAGTACATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC10.5813953488372093No Hit
CACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTC10.5813953488372093No Hit
CTGATGGTAACAGCATGGGGGAAAACTCATCAAAGTTGATGGTGCTGAGG10.5813953488372093No Hit
ACGGTAAACAACACGATCTTCCCCTACGACCAGCAGACAGGGACATCCAT10.5813953488372093No Hit
CGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCT10.5813953488372093No Hit
GTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGT10.5813953488372093No Hit
GTCATATGTCGCGGGTTCTGCACATATCACGGACGTATCGCTATCAGACG10.5813953488372093No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT10.5813953488372093No Hit
GAGTACATGGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATG10.5813953488372093No Hit
GAGTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG10.5813953488372093No Hit
GCCATTTCTGAAGCCAGCTGTTCCTGGGTCTTCTTCTGGTCCCGAGAAGC10.5813953488372093No Hit
GGGTGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGTTCTTTT10.5813953488372093No Hit
CAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGC10.5813953488372093No Hit
GGCTGGCACTGGACCAAAGGATGGAGGCACTCATCACCGCCCTGGAGGAA10.5813953488372093No Hit
CCAGTCTCCCATCTTCAGGCTCCTTTTTCTGAGCTGTGTCCACAAAAGCA10.5813953488372093No Hit
TGGCGACAGTCCATCATTAAAGATGAGGAGATCAGCTTCAAGCTCTTCCA10.5813953488372093No Hit
GCTCTATATTTCTCTTTCTTTACCTTCTCTTTTGAAGGATATTCATTTAA10.5813953488372093No Hit
CTCTTATCTACAGCAGAAACATTGAGGATGCCATTGGCATCGATGTCAAA10.5813953488372093No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG10.5813953488372093No Hit
TAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATT10.5813953488372093No Hit
AGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGT10.5813953488372093No Hit
GGTCATAGGGGCAGGGGTTGGAATGTGGCTCCGAGTCTTCTGGGCGGCCA10.5813953488372093No Hit
CCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACG10.5813953488372093No Hit
CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA10.5813953488372093No Hit
GGCTGAAGCAGTCAAGATGACCTTATTTCAGCTTCTACGTGAGCACTGGG10.5813953488372093No Hit
CCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGCCGAGCTTAGAGCG10.5813953488372093No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG10.5813953488372093No Hit
GCTGTGTCACCTGCCTGGTTCCCTTCAATGGGTGGATTTGTTTCACCATT10.5813953488372093No Hit
GATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAA10.5813953488372093No Hit
CATTACAGGCACTGCACTTCCCAGTGCGCTGATTAAGGATCACTGAGAAT10.5813953488372093No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC10.5813953488372093No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT10.5813953488372093No Hit
GAACAGCAGACACCTTTATTACACTACACATGAGGCTCATTTGCCTTTCC10.5813953488372093No Hit
TTCCAGGGCAATCGCTTCAATTCCAAGATAATCTTAAAACATAAGACAAT10.5813953488372093No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG10.5813953488372093No Hit
GCCGTATGCGGCGGTTGTTCTGTGATCTACTAGCGCCAATCTATATTACT10.5813953488372093No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT10.5813953488372093No Hit
TAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGA10.5813953488372093No Hit
GGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGA10.5813953488372093No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT10.5813953488372093No Hit
TTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGA10.5813953488372093No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA10.5813953488372093No Hit
TTTTTTCATGTGCCTGATTTAAAATCGCCTCTGGTGATTTAACCTTTTAT10.5813953488372093No Hit
GGTCTGTTTTAGGCTCTTCTCTGCTTTGTCCACAAAGTCTTTTTCTTCAA10.5813953488372093No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG10.5813953488372093No Hit
GCAGAGTACATGGGGGCCCTGCCCACTCCCCGGCGTCTAAACACAGGTGG10.5813953488372093No Hit
GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT10.5813953488372093No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT10.5813953488372093No Hit
ACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGA10.5813953488372093No Hit
TTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCT10.5813953488372093No Hit
GGCAAGACCATCACCCTAGAGGTGGAGCCCAGTGACACCATCTGTCTCTT10.5813953488372093No Hit
TAATTCTACCACGCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACA10.5813953488372093No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA10.5813953488372093No Hit
GAAGTAGGGACAGGAGGCAGCTGTGAGGCTACAGGTCTTTATCCACAGTG10.5813953488372093No Hit
CATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCT10.5813953488372093No Hit
ACTAATGGCAGCTGCACTCGGATATATTCCACTCAGTGACCCAGTTTCGG10.5813953488372093No Hit
GCCTATGGCTGAGTATGTAACGCTATGGCGTGCTAGTCGTCTCATATACG10.5813953488372093No Hit
GCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGC10.5813953488372093No Hit
ATTCCATGTGGGCCAACTGCCATGTTGTGTCCAGTCGCTATCGGAGTAGC10.5813953488372093No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA10.5813953488372093No Hit
AGTCTATGTAAAGGTTCCTCTGGTGAACTTTGTAGAAAGTTAAAGTCTGT10.5813953488372093No Hit
TTTGTATACTCTACACTGAAGAAATGTAGAATGGCCTACAATAACTCTGC10.5813953488372093No Hit
ATTTTAGGATATTGTGAGTAATATAGATTGGCGCTAGTAGATCACAGAAC10.5813953488372093No Hit
ATCTACAGCAGTAGCAACAATGGAAGTAACCTCAGGTTCAGGAGCAACTG10.5813953488372093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATT10.5813953488372093No Hit
GGGCGTCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGG10.5813953488372093No Hit
GCATAGTGGGACTCGTACCACTGTCGGTACGGCGTGCTGTCAATAAGCAC10.5813953488372093No Hit
TTGTTGCTGAGACATCCACTGGCTGCTTGTTTGCTGGATCATCACTTGGC10.5813953488372093No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTATCTCGTATGCCGTCTTCTGC10.5813953488372093TruSeq Adapter, Index 2 (96% over 25bp)
GTGTAATATCAGCTGCCGTTACAGTTCCTTCACCCTGTACATCAATTTCT10.5813953488372093No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT10.5813953488372093No Hit
ACGCAGAGTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAA10.5813953488372093No Hit
TCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTC10.5813953488372093No Hit
GTGCTGAGTCTTGGGGTACTGGCAGAGCTGTGGCTCGAATTCCAAGAGTT10.5813953488372093No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC10.5813953488372093No Hit
AGTTTCGGCCACGCTCCTATGTTTTGCTGGTAGATACCGGCGCGCTAGCA10.5813953488372093No Hit
GTACACGGGGGAATTCAGATAAAACGAATAGCTCGTAACCCAACATGCAC10.5813953488372093No Hit
CAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTG10.5813953488372093No Hit
CTTATACACATCTCCGAGCCCACGGACTAGGCATGATCTCGTATGCCGTC10.5813953488372093No Hit
GACTGATGGAAGCACTGGACCGAAAGAAGCAATTGCGCTTGGTTCAAAGA10.5813953488372093No Hit
AAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAG10.5813953488372093No Hit
CTGTGGATTGCCGGCATTGAGCCCACGGTAAACCCCAAATACATAAGTGT10.5813953488372093No Hit
CCATCTATCTGGATTGATGTTACAGCGGCACCAGGGAGTGAGGATAAGAG10.5813953488372093No Hit
GCGCAATACATATCATGCGAGCGTATTATCAGACATCGCACGCACGATCA10.5813953488372093No Hit
TCCTTATACAGTACGCAGCGACCGTAACGCTGTATGAGATCGCCGCATCC10.5813953488372093No Hit
GACTACACCCCTGGGACACAGGATAATTACTGAGACGGGAAGAGGGGACA10.5813953488372093No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT10.5813953488372093No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCTTTTTTAA10.5813953488372093No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC10.5813953488372093No Hit
AAGATGAGGAGATCAGCTTCAAGCTCTTCCACGAGTGCCTTCAGCTCTTC10.5813953488372093No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCTTTGGCTTCCTTCCGCAG10.5813953488372093No Hit
GTTTGTGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG10.5813953488372093No Hit
CCTTCAGGACCTTAACTCACTTCATAATTGTTTAAGTCTGTCTGATCACC10.5813953488372093No Hit
CACCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT10.5813953488372093No Hit
TAATATGACAGGCCGCTAAAGACGTTCTACTCCGCCATATGAGATAATAT10.5813953488372093No Hit
CGCTGGTGCCACACGACTACAACCCTCGTAATAGGGCTGCGTGCGTCCTA10.5813953488372093No Hit
GGCTTCAGATGAGCTCTCTCGTACATATCCTGAGCCTTGAGACTATAACA10.5813953488372093No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC10.5813953488372093No Hit
GGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGG10.5813953488372093No Hit
GAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTG10.5813953488372093No Hit
TTATATAGTAGATTATAAAGTCTATGCAATTAAGAATGTCCACTGATTTC10.5813953488372093No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGTCAAGTCAGC10.5813953488372093No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA10.5813953488372093No Hit
TATGAGAACAGCTGCTGCTTCAGTGAAGGGTTTCTGGCATAACCAATGAT10.5813953488372093No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT10.5813953488372093No Hit
GAAGACGCTAGAAATTGATGTACAGGGTGAAGGAACTGTAGCGGCAGCTG10.5813953488372093No Hit
CACATGGTTACGCTTTGGGGAAATTATGAGGGGATCTCTCAGTGCTTTGC10.5813953488372093No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA10.5813953488372093No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC10.5813953488372093No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA10.5813953488372093No Hit
CCATTTTTCTGGTCATCGGGTCCCGCGTGACAAACAGGAGGTCTTCTTCA10.5813953488372093No Hit
GAGTACATGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGG10.5813953488372093No Hit
TTGTTAGGGGTCAGCCATTTTTAATGATAACGGGTGACCAAACCAGCCTT10.5813953488372093No Hit
GATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCG10.5813953488372093No Hit
GCTTAACACACGCCCAGTCTCCTCCAGGTCAACAGACGTGTCAGCTCCCA10.5813953488372093No Hit
GCCTTATGTGATTGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA10.5813953488372093No Hit
ATACTAAAATTATCTGTGGATTGCCGGCATTGAGCCCACGGTAAACCCCA10.5813953488372093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5813953488372093No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA10.5813953488372093No Hit
TTCGAGCCCCGCGCGTACTAGGCAGATAAGAACCCTCCAGCTCGGGGCCT10.5813953488372093No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT10.5813953488372093No Hit
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG10.5813953488372093No Hit
GTCGTCTACCAGCTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCAC10.5813953488372093No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC10.5813953488372093No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT10.5813953488372093No Hit
ATGGGTGAGCATGATCGAGCTAGTGGATCAGATATCCAGGTAGTGAACTG10.5813953488372093No Hit
ATATAATTTTTGAGGTTCAGAGACGTTCATCATATCCAAATAGCTGATTA10.5813953488372093No Hit
CCCACTGGGCGAACAGCAACGTTATAACGGCCACTCAGTGGTTCGTCACG10.5813953488372093No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT10.5813953488372093No Hit
ATCTCATAGTCACCACATAGATCGCGACATATCAGCATACATGGCATACT10.5813953488372093No Hit
CTCATAATCCTTCTCTAGGGCAGCCATGTCCTCACGGGCCTCAGAGAACT10.5813953488372093No Hit
CAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCT10.5813953488372093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5813953488372093No Hit
CTGTTTACAGTAGTAAAGGACAACAGATAATGTACAAACCGGTGAGTAAA10.5813953488372093No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG10.5813953488372093No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT10.5813953488372093No Hit
CTCTACGGAGTGTGCTCGGCTATAACAGCGAGTGCTACGCCTAAACTGGC10.5813953488372093No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA10.5813953488372093No Hit
CACTCACAGTGATGCAATGTGAAGAGGCAAAGCGAGCAAATGTATTTTTC10.5813953488372093No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT10.5813953488372093No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT10.5813953488372093No Hit
GTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAG10.5813953488372093No Hit
TCCCTCCATATCTCCCAGAAGGTACCGCGAGCCATTAGGATCCACCCGGT10.5813953488372093No Hit
CCTATTGGGATACCTCCCGTCCATTAAGTTAGGGCTTTCAGCCCTAATTA10.5813953488372093No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)