FastQCFastQC Report
Thu 26 May 2016
ERR580297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13616299
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281960.2070753587300044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219950.16153434938524777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200500.047.4676781
GTATCAA355850.040.6595341
CAACGCA403700.034.5312165
ATCAACG410650.033.8922963
TATCAAC442550.032.3962942
AACGCAG447650.031.7033586
TCAACGC457550.030.6748244
GTACTAG76100.029.0412331
ACGCAGA493850.028.099837
CGCAGAG511600.027.0048988
GTGGTAT84500.026.432531
GTACATG537150.025.1414721
TACATGG535100.024.6928732
GCAGAGT562050.023.9824629
ACATGGG543800.023.3715723
TAGTACT112900.023.030724
CTGTGCG112200.022.9968879
CTAACGC35300.022.9105153
TAGGCAT107100.022.827585
GCATCGT86200.022.569348
Produced by FastQC (version 0.11.5)