FastQCFastQC Report
Thu 26 May 2016
ERR580297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13616299
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262440.19273959832991328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216030.1586554466819508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196400.047.2112241
GTATCAA346300.041.034571
CAACGCA409050.033.5138785
ATCAACG412750.033.1690033
TATCAAC442900.031.9710222
AACGCAG453450.030.801126
TCAACGC459600.030.0531884
GTACTAG74850.029.4258041
ACGCAGA504300.026.987137
GTGGTAT81850.026.7939971
CGCAGAG524350.025.8477468
GTACATG548700.024.4884191
TACATGG551100.023.8179042
GCAGAGT578000.023.0665049
ACATGGG550850.022.9764023
TAGGCAT105350.022.7955995
CTGTGCG111800.022.1523139
TAGTACT113250.021.7452354
CTAGTAC118250.021.5813033
CTAGGCA114200.021.3997294
Produced by FastQC (version 0.11.5)